Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 390
Filtrar
1.
Dev Comp Immunol ; 126: 104207, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34273355

RESUMO

Infectious bacterial and viral diseases that cause hemolysis are considered life-threatening to grass carp (Ctenopharyngodon idellus), which is a species used in aquaculture worldwide. After heme and hemeproteins (Hb) are released as a result of hemolysis, the effect of excess Hb and heme on tissues remains to be characterized. To decipher the mechanisms, after incubation with Hb, we showed that lipopolysaccharide (LPS), Hb, and heme increased the cytotoxicity and secretion of inflammatory cytokines such as interleukin (IL)-6, chemokine (C-C motif) ligand 1 (CCL1), tumor necrosis factor (TNF)-α, IL-6, and IL-1ß in vitro, which was due to stimulation of the expression of innate immune receptors, such as nucleotide-binding oligomerization domain (NOD2), toll-like receptor 2 (TLR2), TLR 4, and TLR3. The formation of reactive oxygen species (ROS) and the activation of mitogen-activated protein kinases (MAPKs) and nuclear factor (NF)-κB were important for increasing the cytokine production to induce heme, Hb, and LPS. Moreover, we confirmed that after LPS, Hb, and heme challenge, superoxide dismutase (SOD) and glutathione (GSH) synthetase (GSS) also caused remarkable destruction. However, catalase (CAT) and heme oxygenase-1 (HO-1) were strongly activated. In summary, our research findings present a framework through which heme and Hb concentrations amplify the secretions of inflammatory cytokines, which are induced by pattern recognition receptor (PRR) activation and present possible paths for immune intervention during infection with viral diseases and hemolytic bacterial.

2.
Molecules ; 26(22)2021 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-34833975

RESUMO

The activation of hepatic stellate cells (HSC) plays a key role in the progression of hepatic fibrosis, it is essential to remove activated HSC through apoptosis to reverse hepatic fibrosis. Schisandrin B (Sch B) is the main chemical component of schisandrin lignan, and it has been reported to have good hepatoprotective effects. However, Schisandrin B on HSC apoptosis remains unclear. In our study, we stimulated the HSC-T6 and LX-2 cell lines with TGF-ß1 to induce cell activation, and the proliferation and apoptosis of the activated HSC-T6 and LX-2 cells were detected after treatment with different doses of Schisandrin B. Flow cytometry results showed that Sch B significantly reduced the activity of activated HSC-T6 and LX-2 cells and significantly induced apoptosis. In addition, the cleaved-Caspase-3 levels were increased, the Bax activity was increased, and the Bcl-2 expression was decreased in HSC-T6 and LX-2 cells treated with Sch B. Our study showed that Sch B inhibited the TGF-ß1-induced activity of hepatic stellate cells by promoting apoptosis.

3.
Chemosphere ; : 133004, 2021 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-34826440

RESUMO

Molybdenum disulfide (MoS2) is a transition metal dichalcogenides (TMDCs) material that is seeing rapidly increasing use. The wide range of applications will result in significant environmental release. Here, the impact of MoS2 nanosheets on rice and associated soil microbial communities was evaluated. Rice plants were grown for 4 weeks in a natural paddy soil amended with either 1T or 2H phase MoS2 nanosheets at 10 and 100 mg kg-1. The 1T MoS2 nanosheets have a significantly greater dissolution rate (58.9%) compared to 2H MoS2 (4.4%), indicating the instability of 1T MoS2 in environment. High dissolution rate resulted in a high Mo bioaccumulation in rice leaves (272 and 189 mg kg-1 under 1T and 2H exposure at 100 mg kg-1). However, this did not induce overt phytotoxicity, as indicated by a range of phenotypic or biochemical based determine endpoints, e.g., biomass, photosynthetic pigments, and malondialdehyde (MDA) content. Additionally, rice P uptake was significantly increased upon exposure to 1T and 2H MoS2 (10 mg kg-1). Gas chromatography-mass spectrometry (GC-MS) reveals that both phases of MoS2 in soil systematically enhanced the carbon and nitrogen related metabolic pathways in exposed plants. Soil 16S rRNA gene sequencing data show that soil microbial community structure was unchanged upon MoS2 exposure. However, both phases of MoS2 remarkably increased the relative abundance of N2-fixation cyanobacteria, and 2H MoS2 exposure increased a plant growth-promoting rhizobacteria-Bacillus. Overall, our results suggest that MoS2 nanosheets at tested doses did not exert negative impacts on rice plant and the associated soil microbial community.

4.
Sci Total Environ ; : 151896, 2021 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-34826474

RESUMO

In this study, the performance of AgNPs-priming (20, 40, and 80 mg/L) on the seed germination, yield, and nutritional quality of Chinese cabbage were evaluated. We found that AgNPs-priming at 20 and 40 mg/L for 15 h significantly accelerated seed germination speed and seedling development. Cabbage seeds primed with different concentrations of AgNPs (0, 20, 40, and 80 mg/L) were then planted in a real soil and allowed to grow for 1 month in greenhouse. Results showed that AgNPs-priming at 40 mg/L significantly increased cabbage yield by 44.3%. Gas chromatography-mass spectrometry (GC-MS) combining with sparse partial least squares-discriminant analysis (sPLS-DA) reveals that AgNPs priming altered the metabolite profile of cabbage leaves in a dose-dependent manner, decreasing carbohydrates and increasing nitrogen related compounds. This indicates that the metabolic stimulation during germination stage can influence the entire life cycle of cabbage. The nutritional quality of cabbage edible leaves was evaluated by liquid chromatography with tandem mass spectrometry (LC-MS/MS) and inductively coupled plasma-mass spectrometry (ICP-MS). Results showed that AgNPs-priming at all tested concentrations significantly increased the content of essential amino acids for several folds in cabbage leaves, including alanine, aspartic acid, glutamine, glutamic acid, histidine, isoleucine, leucine, lysine, phenylalanine, proline, serine, threonine, tyrosine, and valine. Meanwhile, AgNPs-priming (40 mg/L) significantly increased iron (Fe) content by 23.8% in cabbage leaves. Ag did not bioaccumulate in edible tissues, indicating the bio-safety of AgNPs-priming. These results suggest that AgNPs-priming is a low-cost and eco-friendly approach to increase crop yield and nutritional quality.

5.
Sci Rep ; 11(1): 21374, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34725402

RESUMO

The high-precision 3D simulation model for geomechanics of a complex coal seam is the necessary premise for the research on intelligent shearer and unmanned mining. However, at present, a simulation model for geomechanics of a complex coal seam generally has the problems of simplifying complex geological structures and low accuracy for structures. In order to meet the needs of a coal seam simulation model in the mining process of an intelligent shearer, it is necessary to optimize the simplified model of a coal seam. Therefore, based on a 3D simplified simulation model constructed with discrete element technology, the complex coal seam application plug-in was compiled with the help of an Application Program Interface. Moreover, according to the geological characteristics, new attributes were added to the structures to complete the construction of the model of a complex coal seam. Finally, the model was verified with laboratory experiments. The results showed that the high-precision 3D simulation model for geomechanics of a complex coal seam effectively improved the accuracy of the modeling. The real-time transmission and the real-time sharing of multi-source data were realized by considering the 3D simulation model for geomechanics of a complex coal seam as the core. Additionally, the purpose of the real-time sensing of the coal cutting state was achieved in order to lay the foundation for the realization of unmanned mining.

6.
Front Med (Lausanne) ; 8: 741312, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34790676

RESUMO

Background: Catheter malfunction is a common complication of peritoneal dialysis (PD). This study aimed to retrospectively analyze the risk factors and management of catheter malfunction in urgent-start PD. Methods: Patients who underwent urgent-start PD were divided into catheter-malfunction and control groups. Baseline demographic and laboratory data of the two groups were compared, and the risk factors for catheter malfunction were analyzed. Primary outcome measure was catheter survival, and the secondary outcomes were surgical complications and malfunction treatment. Results: Total of 700 patients was analyzed, among whom 143 (20.4%) experienced catheter malfunctions, specifically catheter migration (96, 67.1%), omental wrapping (36, 25.2%), and migration plus omental wrapping (11, 7.7%). Catheter survival time in the malfunction group (202.5 ± 479.4 days) was significantly shorter than that in the control group (1295.3 ± 637.0 days) (P < 0.001). Multivariate analysis revealed higher body mass index [hazard ratio (HR), 1.061; 95% confidence intervals (CI), 1.010-1.115; P = 0.018], lower surgeon count (HR, 1.083; 95% CI, 1.032-1.136; P = 0.001), and higher serum potassium (HR, 1.231; 95% CI, 1.041-1.494; P = 0.036) as independent risk factors for catheter malfunction, while older age (HR, 0.976, 95% CI, 0.962-0.991; P = 0.002) and colonic dialysis (HR, 0.384; 95% CI, 0.254-0.581; P < 0.001) as protective factors. Further subgroup analysis revealed a shorter catheter survival time in patients with younger age ( ≤ 40 years), higher serum potassium levels (≥5 mmol/L), while a longer catheter survival time in patients with colonic dialysis. PD tube and subcutaneous tunnel preservation was successful in 41 out of 44 patients with omental wrapping. All patients had good post-incision prognoses. Conclusions: Urgent-start PD is safe and effective for unplanned PD patients. Adequate pre-operative colonic dialysis and serum potassium level control are conducive in preventing catheter malfunction. Conservative treatment is effective in managing catheter migration alone, while preservation of the PD tube and the subcutaneous tunnel is effective for omental wrapping.

7.
Front Med (Lausanne) ; 8: 731955, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34671619

RESUMO

Introduction/aim: The supraphysiologic chloride concentration of normal saline may contribute to acute kidney injury (AKI). Balanced crystalloids can decrease chloride concentration and AKI in critically ill patients. We aim to test the hypothesis that, in patients with predicted severe acute pancreatitis (pSAP), compared with saline, fluid therapy with balanced crystalloids will decrease plasma chloride concentration. Methods/Design: This is a multicenter, stepped-wedge, cluster-randomized, controlled trial. All eligible patients presenting to the 11 participating sites across China during the study period will be recruited. All sites will use saline for the first month and sequentially change to balanced crystalloids at the pre-determined and randomly allocated time point. The primary endpoint is the plasma chloride concentration on day 3 of enrollment. Secondary endpoints will include major adverse kidney events on hospital discharge or day 30 (MAKE 30) and free and alive days to day 30 for intensive care admission, invasive ventilation, vasopressors, and renal replacement therapy. Additional endpoints include daily serum chloride and sequential organ failure assessment (SOFA) score over the first seven days of enrollment. Discussion: This study will provide data to define the impact of normal saline vs. balanced crystalloids on plasma chloride concentration and clinical outcomes in pSAP patients. It will also provide the necessary data to power future large-scale randomized trials relating to fluid therapy. Ethics and Dissemination: This study was approved by the ethics committee of Jinling Hospital, Nanjing University (2020NZKY-015-01) and all the participating sites. The results of this trial will be disseminated in peer-reviewed journals and at scientific conferences. Trial registration: The trial has been registered at the Chinese Clinical Trials Registry (ChiCTR2100044432).

8.
Blood Purif ; : 1-11, 2021 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-34673634

RESUMO

BACKGROUND: Anticoagulation-free continuous renal replacement therapy (CRRT) was recommended by the current clinical guideline for patients with increased bleeding risk and contraindications of citrate. Nevertheless, anticoagulation-free CRRT yielded heterogeneous filter lifespan. Furthermore, the specific cutoff values for traditional coagulation parameters to predict sufficient filter lifespan of anticoagulation-free CRRT have not yet been determined. The purpose of our present study was to develop and validate a model for predicting sufficient filter lifespan in anticoagulation-free CRRT patients. METHODS: Patients who underwent anticoagulation-free CRRT in our center between June 2013 and June 2019 were retrospectively included. The primary outcome was sufficient filter lifespan (≥24 h). Thirty-seven predictors were included for modeling based on their clinical significance and previous reports. The final model was developed by using multivariable logistic regression analysis and was validated in a separate external cohort. RESULTS: The development cohort included 170 patients. Sufficient filter lifespan was observed in 80 patients. Thirteen variables were independent predictors for sufficient filter lifespan by logistic regression: body temperature, mean arterial pressure, activated partial thromboplastin time, direct bilirubin, alkaline phosphatase, blood urea nitrogen, vasopressor use, body mass index, white blood cell, platelet count, D-dimer, uric acid, and pH. The area under the curve (AUC) of the stepwise model and internal validation model was 0.82 (95% confidence interval [CI] [0.76-0.88]) and 0.8 (95% CI [0.74-0.87]), respectively. The positive predictive value and the negative predictive value of the stepwise model were 0.77 and 0.79, respectively. The validation cohort included 44 eligible patients and the AUC of the external validation model was 0.82 (95% CI [0.69-0.96]). CONCLUSIONS: The use of a prediction model instead of an assessment based only on coagulation parameters could facilitate the identification of the patients with filter lifespan of ≥24 h when they accepted anticoagulation-free CRRT.

9.
Ren Fail ; 43(1): 1378-1393, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34602015

RESUMO

OBJECTIVE: The aim of this study was to determine the efficacy and safety of lanthanum carbonate (LC) versus calcium salts, non-LC phosphate binders (PBs), sevelamer, or placebo in patients with chronic kidney disease (CKD). MATERIALS AND METHODS: A literature search on PubMed, Embase, and Cochrane Library databases was conducted up to 18 June 2021. Data acquisition and quality assessment were performed by two reviewers. Meta-analysis was performed to evaluate the serum biochemical parameters, adverse events, and patient-level outcomes of LC, non-LC PBs, and sevelamer for hyperphosphatemia in patients with CKD. Heterogeneity across studies was assessed utilizing the I2 statistic and Q-test, and a random effect model was selected to calculate the pooled effect size. RESULTS: A total of 26 randomized, controlled trials and 3 observational studies were included. Compared to the other groups, better control effect of serum phosphorus (RR = 2.68, p < 0.001), reduction in serum phosphorus (95%CI = -1.93, -0.99; p < 0.001), Ca × P (95%CI = -13.89, -2.99; p = 0.002), serum intact parathyroid hormone levels (95%CI = -181.17, -3.96, p = 0.041) were found in LC group. Besides, reduced risk of various adverse effects, such as hypotension, abdominal pain, diarrhea, dyspepsia, and a score of coronary artery calcification were identified with LC in comparison to calcium salt, non-LC PBs, or placebo group. Significantly lower risk in mortality with LC treatment vs. non-LC PBs was observed, while no significant difference was identified between LC and calcium salt groups. CONCLUSION: LC might be an alternative treatment for hyperphosphatemia in patients with CKD considering its comprehensive curative effect.

10.
Inorg Chem ; 60(21): 16645-16651, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34661398

RESUMO

The structures and chemical bonding of Ge3C- and Ge3O- as well as their neutrals are explored with anion photoelectron spectroscopy and theoretical calculations. The vertical detachment energies of Ge3C- and Ge3O- are measured to be 1.51 ± 0.04 and 2.00 ± 0.04 eV, respectively. It is found that Ge3C-/0 have a C2v symmetric planar structure with the C atom interacting with three Ge atoms. Ge3O-/0 have the O atom interacting with two Ge atoms of the triangular Ge3 unit. Ge3O- has a Cs symmetric nonplanar structure, while Ge3O has a C2v symmetric planar structure. Theoretical results show that the multiconfigurational effects in Ge3C-/0 and Ge3O-/0 are insignificant. Chemical bonding analyses reveal that there exist the C-Ge3 π⊥ orbital interaction and two π aromatic Ge2C units in Ge3C. There are O-Ge3 π⊥ orbital interaction and one doubly aromatic Ge3 unit in Ge3O, but the π⊥ orbital interaction is relatively weak.

11.
Phytomedicine ; 93: 153790, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34710756

RESUMO

BACKGROUND: Sijunzi decoction (SJZD), reported in "Tai Ping Hui Min He Ji Ju Fang" of the Song dynasty, is the basic prescription for the treatment of spleen deficiency syndrome (SDS) in traditional Chinese medicine (TCM). It is composed of Ginseng Radix et Rhizoma, Atractylodisa Macricephalae Rhizoma, Poria and Glycyrrhizae Radix et Rhizoma Praeparata Cum Melle. PURPOSE: This study sought to explore the effects of different components in SJZD (including nonpolysaccharide NPS and active polysaccharide S-3) on SDS rats and their underlying mechanisms. STUDY DESIGN AND METHODS: First, SDS model rats were established by reserpine injection and then treated with SJZD, NPS and S-3. To clarify their effect on GI motility and immune function, the gastrointestinal (GI) hormone levels in rat serum and their related receptor expressions in rat intestine were detected by enzyme-linked immunosorbent assay (ELISA) and western blot, and the intestinal T lymphocyte expression were quantified by flow cytometry. The levels of SCFAs in feces and serum were measured by gas chromatography-mass spectrometry (GC-MS), and the gut microbiota composition was determined by 16S RNA sequencing. Furthermore, pseudo-germ-free (pGF) and gut microbiota dysbiosis (GMD) model rats were established to verify the key role of the gut microbiota in the treatment of SDS with SJZD, NPS and S-3. RESULTS: SJZD has a stronger therapeutic effect on intestinal immune and GI hormone secretion in SDS rats, while the efficacy of NPS and S-3 showed slight differences. NPS mainly regulated the secretion of GI hormones in SDS rats and directly improved intestinal immunity by increasing the expression of T lymphocyte cells, while S-3 mainly enhanced intestinal immune function by increasing the expression of T lymphocyte cells and repairing the intestinal barrier in both direct and indirect ways. Additionally, experiments in pGF and GMD rats have proven that the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats and the regulation of GI hormones of S-3 are related to modulation of the gut microbiota composition, while the regulation of GI hormones by SJZD and NPS is not completely dependent on this modulation. In particular, Lactobacillus, SMB53, Blautia, Dorea, Collinsella and Adlercreutzia were significantly modulated by SJZD, and 3 genera (including Lactobacillus, Dorea and SMB53) were also remarkably regulated by NPS. S-3 significantly increased the abundance of Butyricimonas and Collinsella, which were different from altered genera in the SJZD group. CONCLUSION: This study uncovered that NPS and S-3 are inseparable effective substances for SJZD in the treatment of SDS rats, in which NPS mainly improves intestinal motility dysfunction and S-3 mainly enhances intestinal immunity. The mediation effect of the gut microbiota is extremely important, but the regulating effect of NPS on gastrointestinal hormones has nothing to do with the gut microbiota.


Assuntos
Medicamentos de Ervas Chinesas , Baço , Animais , Medicamentos de Ervas Chinesas/farmacologia , Masculino , Polissacarídeos/farmacologia , Ratos
12.
Eur Spine J ; 2021 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-34545441

RESUMO

PURPOSE: To quantify the degree of available space for the cord and cord swelling in patients following traumatic cervical spinal cord injury (TCSCI), and to assess the relationship among the available space for the cord, cord swelling, and the severity of neurological impairment. METHODS: This study included 91 patients. The following indexes were measured by two blinded observers: maximum cord available area (CAAmax) and maximum cord swelling area (CSAmax). The American Spinal Injury Association (ASIA) impairment scale (AIS) grades were used to evaluate the extent of neurological injury. Relationship among CAAmax, CSAmax, and initial AIS grades was assessed via univariate and multivariate analyses. RESULTS: Patients who were AIS grade A (complete injury) demonstrated significantly greater median CAAmax and CSAmax than AIS grade C or D (incomplete injury) (P < 0.01). Multivariate analysis identified only CAAmax (OR 20.88 [95% CI 1.50-291.21]; P = 0.024) and CSAmax (OR 17.84 [95% CI 1.15-276.56]; P = 0.039) were identified as independently influencing the likelihood of complete injury at the initial assessment. The classification accuracy was best for CAAmax and CSAmax; areas under the curve were 0.8998 (95% CI 0.7881-1.0000) and 0.9167 (95% CI 0.8293-1.0000), respectively. CONCLUSION: The present study provides a novel radiologic method for identifying the severity of TCSCI with T2-weighted MRI findings. Greater available space for the cord (CAAmax > 38%) and cord swelling (CSAmax > 29%) can be used to identify patients at risk for TCSCI and both imaging characteristics are associated with an increased likelihood of severe neurological deficits. LEVEL OF EVIDENCE: Diagnostic: individual cross-sectional studies with consistently applied reference standard and blinding.

13.
Biomed Pharmacother ; 143: 112160, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34560546

RESUMO

Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease. Bu-Shen-Tong-Du prescription (BSP) has traditionally been used in to treat RA but its underlying mechanisms remain unclear. In this study, we explored the potential mechanisms of BSP in collagen-induced arthritis (CIA) rats, a classic animal model of RA. We employed an integrated pharmacology approach in combination with network pharmacology, 1H-nuclear magnetic resonance (NMR) metabolomics, and biochemical analyses to determine the mechanisms of BSP for treating RA. We found that BSP can regulate immunity and inflammation by decreasing the spleen index; inhibiting hyperplasia of the white pulp; reducing the levels of IL-1ß, IL-6, IL-17A, and IFN-γ; and increasing the levels of IL-10 in the serum. Network pharmacology was utilized to predict related signal transduction pathways of BSP in RA treatment. 1H NMR metabolomics of the serum confirmed that BSP regulated energy metabolism and amino acid metabolism. Finally, we validated the Toll-like receptor 4 (TLR4)/nuclear factor (NF)-κB signaling pathway using immunohistochemical methods, which demonstrated that BSP controlled RA-induced inflammation by inhibiting the TLR4/NF-κB signaling pathway. These results confirm the therapeutic effect of BSP in a CIA rat model, which is exerted via the inhibition of the inflammation and the improvement of the immune function, balancing energy metabolism and amino acid metabolism, and inhibiting the TLR4/NF-κB signaling pathway. This study provides an experimental basis for using BSP as a combinatorial drug to inhibit inflammation and regulate immunity in the treatment of RA.

14.
ACS Nano ; 15(10): 16344-16356, 2021 10 26.
Artigo em Inglês | MEDLINE | ID: mdl-34569785

RESUMO

Fully understanding the environmental implications of engineered nanomaterials is crucial for their safe and sustainable use. Cyanobacteria, as the pioneers of the planet earth, play important roles in global carbon and nitrogen cycling. Here, we evaluated the biological effects of molybdenum disulfide (MoS2) nanosheets on a N2-fixation cyanobacteria (Nostoc sphaeroides) by monitoring growth and metabolome changes. MoS2 nanosheets did not exert overt toxicity to Nostoc at the tested doses (0.1 and 1 mg/L). On the contrary, the intrinsic enzyme-like activities and semiconducting properties of MoS2 nanosheets promoted the metabolic processes of Nostoc, including enhancing CO2-fixation-related Calvin cycle metabolic pathway. Meanwhile, MoS2 boosted the production of a range of biochemicals, including sugars, fatty acids, amino acids, and other valuable end products. The altered carbon metabolism subsequently drove proportional changes in nitrogen metabolism in Nostoc. These intracellular metabolic changes could potentially alter global C and N cycles. The findings of this study shed light on the nature and underlying mechanisms of bio-nanoparticle interactions, and offer the prospect of utilization bio-nanomaterials for efficient CO2 sequestration and sustainable biochemical production.


Assuntos
Molibdênio , Nostoc , Carbono , Nitrogênio
15.
Carbohydr Polym ; 272: 118445, 2021 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-34420710

RESUMO

Gas pollution is a serious problem. More attention has been paid to the detection and monitoring of toxic and harmful gases, and it is urgently needed for a sensor that could simultaneously identify and distinguish between acid and base gases. Based on the electrostatic interaction resulting from amidogen of chitosan (CS) and carboxylic groups of itaconic acid (IA), we successfully prepared a series of biomass aerogels (CS-IA aerogels) that could respond to acidic and alkaline gases with different concentrations. The acidic and alkaline gases could be easily detected and distinguished by changing the content of IA in CS-IA aerogels. Moreover, the electrostatic interactions could also endowed CS-IA aerogels with self-healing ability in the breaks at room temperature. After self-healing, CS-IA aerogels still sensitively answered to acidic and alkaline gases. CS-IA aerogels with sensitivity to acid-base gas and self-healing performance has been suggested to be promising candidates as application of multi-functional aerogels.

17.
Medicine (Baltimore) ; 100(33): e26850, 2021 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-34414935

RESUMO

BACKGROUND: Gastric cancer (GC) is a strong cause of global cancer mortality. Nucleotide excision repair (NER) can modulate platinum-based chemotherapeutic efficacy by removing drug-produced DNA damage. Some studies have found a link between excision repair cross complementation group 1 (ERCC1) rs2298881, one gene in NER pathway, and response to chemotherapy. However, the results have been disputed. METHODS: We conducted a meta-analysis to reevaluate the association between polymorphisms of NER gene (ERCC1 rs2298881) and the clinical outcomes in gastric cancer patients receiving platinum-based chemotherapy. Searching PubMed, Web of Science, EMBASE, Google Scholar, and China National Knowledge Infrastructure, 2 independent searchers found all pertinent literatures up to May 1, 2021. We enrolled studies according to consistent selection criteria, extracted and vitrified data. Crude odds ratios (ORs) and hazard ratios (HRs) with 95% confidence interval (CI) were applied to evaluate the effect of ERCC1 rs2298881 on patients treated by platinum-based chemotherapy. RESULTS: By the data gathered from 6 independent studies, 1940 cases diagnosed with gastric cancer and treated with chemotherapy were included, containing 1208 Good-Responders and 732 Poor-Responders. With a comprehensive meta-analysis, we found that the patients with ERCC1 rs2298881A allele had a worse response to chemotherapy than those who with rs2298881C allele under allelic model (A vs C), with the pooled OR of 0.780 (95% CI: 0.611-0.996, P = .046). And our analysis indicated that AA genotype was associated with unfavorable overall survival (HR = 1.540, 95% CI = 1.106-2.144, P = .011) compared with CC genotype. CONCLUSIONS: ERCC1 rs2298881 is suggested as a marker of clinical outcome in gastric cancer patients treated by platinum-based chemotherapy.


Assuntos
Antineoplásicos/uso terapêutico , Cisplatino/uso terapêutico , Proteínas de Ligação a DNA/genética , Endonucleases/genética , Oxaliplatina/uso terapêutico , Neoplasias Gástricas/tratamento farmacológico , Neoplasias Gástricas/genética , Humanos , Polimorfismo de Nucleotídeo Único , Prognóstico , Neoplasias Gástricas/mortalidade
18.
Fish Shellfish Immunol ; 118: 94-101, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34450271

RESUMO

Superoxide dismutases (SODs) are the main antioxidant enzymes involved in alleviating oxidative stress. Although mitochondrial manganese SOD (mMnSOD) has been reported to be correlated with the immune response in crustaceans, its biological properties and role in the immune response remain unclear. Here, we cloned the Macrobrachium rosenbergii mMnSOD (MrmMnSOD), analyzed its activity and expression pattern under Staphylococcus aureus and Vibrio parahaemolyticus infection, and further explored its possible mechanism during antibacterial immune response. The results showed that both enzyme activity and the expression of MrmMnSOD were significantly up-regulated by bacterial infection. MrmMnSOD knockdown made the prawn susceptible to Vibrio infection, which increased the mortality rate and the number of bacteria in haemocytes. The bacterial agglutination assay confirmed that MrmMnSOD decreases bacterial abundance via agglutination. Overall, this work identified antibacterial function of MrmMnSOD in the immune response. In addition to contributing to immunological theory, these findings aid disease prevention and control in crustacean aquaculture.

19.
Am J Transl Res ; 13(7): 7654-7666, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34377242

RESUMO

BACKGROUND: Traditional Chinese Medicine (TCM) massage utilizes mechanical force stimulation, and the amount of mechanical force influences therapeutic outcome. This amount is determined by pressure, frequency, and duration; however, there are no standard definitions for these measures. METHODS: An orthogonal design was used to evaluate massage efficacy using muscle tension as an index. Pressure (2, 4, 6 kg), duration (5, 10, 15 min), frequency (60, 120, 180 repetitions/min), pain (mild, medium, severe), weight (<60, 60-75, >75 kg), and sex (male, female) were evaluated. Additionally, a porcine model of muscle tension was used to construct pressure-time curves for muscle tissues under static and dynamic pressure. RESULTS: We identified an interaction among the six massage measures (P<0.05). Of these measures, only two were individually significant: manipulation frequency and patient pain level (P<0.05). Specifically, 120 repetitions/min improved muscle tension significantly more than 60 or 180 repetitions/min (P<0.05), and patients with severe pain had significantly improved muscle tension compared to those with medium or mild pain (P<0.05). In the porcine muscle model, both static and dynamic pressure were attenuated by approximately 12.5% per cm. This attenuation dropped to 10% per cm when the pressure sensor was placed below tissues with different thicknesses instead of being inserted into tissues at different levels. CONCLUSION: Manipulation frequency and patient pain level were primarily responsible for the therapeutic effects of TCM massage. Mechanistically, pressure was attenuated by nearly 75% at a depth of 2 cm from the muscle surface during TCM massage.

20.
Stem Cell Res Ther ; 12(1): 420, 2021 07 22.
Artigo em Inglês | MEDLINE | ID: mdl-34294152

RESUMO

BACKGROUND: Unresponsive thin endometrium caused by Asherman syndrome (AS) is the major cause of uterine infertility. However, current therapies are ineffective. This study is to evaluate the effect of transplantation with collagen scaffold/umbilical cord mesenchymal stem cells (CS/UC-MSCs) on this refractory disease. METHODS: Eighteen infertile women with unresponsive thin endometrium, whose frozen-thawed embryo transfers (FETs) were cancelled due to reduced endometrial thickness (ET ≤ 5.5 mm), were enrolled in this before and after self-control prospective study. Hysteroscopic examination was performed to confirm no intrauterine adhesions, then twenty million UC-MSCs loaded onto a CS were transplanted into the uterine cavity in two consecutive menstrual cycles. Then uterine cavity was assessed through hysteroscopy after two transplants. FETs were performed in the following cycle. Pregnancy outcomes were followed up. Endometrial thickness, uterine receptivity and endometrial angiogenesis, proliferation and hormone response were compared before and after treatment. RESULTS: Sixteen patients completed the study. No treatment-related serious adverse events occurred. Three months after transplantation, the average ET increased from 4.08 ± 0.26 mm to 5.87 ± 0.77 mm (P < 0.001). Three of 15 patients after FET got pregnant, of whom 2 gave birth successfully and 1 had a miscarriage at 25 weeks' gestation. One of 2 patients without FET had a natural pregnancy and gave birth normally after transplantation. Immunohistochemical analysis showed increased micro-vessel density, upregulated expression of Ki67, estrogen receptor alpha, and progesterone receptor, indicating an improvement in endometrial angiogenesis, proliferation, and response to hormones. CONCLUSION: CS/UC-MSCs is a promising and potential approach for treating women with unresponsive thin endometrium caused by AS. TRIAL REGISTRATION: ClinicalTrials.gov NCT03724617 . Registered on 26 October 2018-prospectively registered, https://register.clinicaltrials.gov/.


Assuntos
Ginatresia , Infertilidade Feminina , Células-Tronco Mesenquimais , Colágeno , Endométrio , Feminino , Ginatresia/terapia , Humanos , Infertilidade Feminina/terapia , Projetos Piloto , Gravidez , Estudos Prospectivos , Cordão Umbilical
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...