Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 7 de 7
Filtrar
Filtros adicionais











País/Região como assunto
Intervalo de ano
1.
Stem Cells Dev ; 28(10): 649-658, 2019 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-30887911

RESUMO

Epigenetic modifications play an important role in neural development. Trimethylated histone H3 at lysine 27 (H3K27me3) is a repressive epigenetic marker that mediates tissue development. In this study, we demonstrate that H3K27me3 and histone methyl transferase Ezh2 regulated the development of dopaminergic (DA) neurons in vitro and in vivo. We found that H3K27me3 increased during differentiation of ventral midbrain-derived neural stem cells (VM-NSCs). However, histone demethylase selective inhibitor GSK-J1 increased H3K27me3 level and decreased the expression of tyrosine hydroxylase. Treated with Ezh2-selective inhibitor EPZ005687 repressed the trimethylation of H3K27 and enhanced differentiation of DA neurons in VM-NSCs cultures. Furthermore, Ezh2 inhibition promoted the expression of DA neurons developmental-related factors by modifying H3K27 trimethylation on the relevant promoter regions. Moreover, the effect of Ezh2 inhibition-mediated DA neurons differentiation was blocked by the expression of shRNA specific for Nurr1. In vivo, Ezh2 decreased and resulted in a reduction of H3K27me3 in developing midbrain. Deletion of Ezh2 by RNA interference approach promoted differentiation of DA neurons during midbrain development. Overexpression of Ezh2 enhanced cell self-renewal and did not affect differentiation of DA neurons.

2.
Sci Rep ; 8(1): 14040, 2018 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-30232349

RESUMO

Oligodendrocytes are a type of glial cells that ensheath multiple neuronal axons and form myelin. Under pathological conditions, such as multiple sclerosis (MS), inflammatory damage to myelin and oligodendrocytes leads to demyelination. Although the demyelinated regions can partially resolve functional deficits through remyelination, however, as the disease progresses, remyelination typically becomes incomplete and ultimately fails. One possible explanation for this failure is the activation of the Notch pathway in MS lesions, which impedes oligodendrocyte precursor cells (OPCs) at maturation. This leads to a potential target for remyelination. Here, we have identified a compound Yhhu4952 that promoted the maturation of cultured OPCs in a dose-dependent and time-dependent manner. Neonatal rats showed a significant increase in the expression of myelin basic protein (MBP) and the prevalence of mature oligodendrocytes in the corpus callosum after Yhhu4952 treatment. The compound was also effective in promoting remyelination in cuprizone-induced demyelination model and improving severity scores in experimental autoimmune encephalomyelitis (EAE) model. Mechanism studies revealed that Yhhu4952 promotes OPC differentiation through the inhibition of the Jagged1-Notch1 pathway. These findings suggest Yhhu4952 is potentially useful for proceeding oligodendrocyte differentiation and remyelination.

3.
BMC Psychiatry ; 17(1): 402, 2017 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-29246120

RESUMO

BACKGROUND: In China, children under 18 years old who are left at rural residences for at least 6 months by either one or both of their parents migrating to work in cities are called "left-behind children (LBC)". Due to restricted family support, they are at a greater risk of developing depressive symptoms than non-left-behind children (NLBC). The objective of this study is to explore how depressive symptoms and stress induced by negative life events such as interpersonal conflicts, punishment and loss, as well as their relationships vary for LBC with different left-behind-related characteristics. METHODS: Using data from a large school-based survey conducted in Chongqing between December 2012 and June 2013, we first identified the differences in depressive symptoms and negative-event-induced stress between LBC and NLBC, and then analyzed the variances among LBC with different left-behind-related characteristics. The data was analyzed with Chi-square test, MANCOVA, ANCOVA, ANOVA, T-test and hierarchical multiple regression analyses. RESULTS: We found that LBC were more stressed when experiencing negative events and had more depressive symptoms than NLBC. Children left behind by both parents were most depressed. Negative-event-induced stress and communication on life difficulties with migrant parents were risk factors for depressive symptoms, whereas adequate communication on academic performance or children's feelings was a protective factor against depressive symptoms. Communication duration and frequency, communication by visiting, communication on academic performance, life difficulties and children's feelings moderated the relationship between stress and depressive symptoms, respectively. Duration of separation, communication duration and frequency, communication on academic performance, learning difficulties and children's feelings moderated the relation between the type of parental migration and depressive symptoms, respectively. CONCLUSIONS: Our findings suggest that children left behind by both parents should be the focus of public attention for their higher susceptibility to stress-related depression. To help LBC stay mentally healthy, governments need to formulate regulations contributing to LBC's family reunion, communities need to involve more residents to attend LBC as "surrogate parents" and teach migrant parents to communicate with LBC properly, and schools need to teach LBC how to deal with stress and communicate with migrant parents.


Assuntos
Comportamento Infantil/psicologia , Depressão/epidemiologia , Divórcio/estatística & dados numéricos , Relações Pais-Filho , Migrantes/estatística & dados numéricos , Criança , China , Comunicação , Divórcio/psicologia , Feminino , Humanos , Masculino , População Rural/estatística & dados numéricos , Fatores Socioeconômicos , Inquéritos e Questionários
4.
Psychiatry Res ; 246: 267-274, 2016 12 30.
Artigo em Inglês | MEDLINE | ID: mdl-27741479

RESUMO

The aim of this study is to determine the prevalence and correlates of depressive symptoms in a school-based sample of 7-17-year-old children and adolescents in west-central China. A large cross-sectional sample survey of 10657 children and adolescents was conducted in Chongqing, a municipality in west-central China. Data were collected from the Children's Depression Inventory (CDI), Adolescent Self-Rating Life Events Checklist (ASLEC) and social-demographic variables which were evaluated with a structured scale. The total prevalence of depression risk was 23.9%. The risk factors for depressive symptoms included age older than 12 years, grade lower than 10, having unmarried parents, being taken care of by people other than two parents (single parent, grandparent(s), other relatives, or others), and living in a low-income family. The pressures of health and adaptation, interpersonal relationship, and study were also strong predictors of depressive symptoms. The prevalence of depressive symptoms among children and adolescents in Chongqing of China is relatively high compared with most figures reported in other cities. There is an urgent need to develop efficacious interventions aimed at the prevention and early recognition of childhood and adolescent depression.


Assuntos
Depressão/epidemiologia , Relações Interpessoais , Fatores Socioeconômicos , Adolescente , Criança , China/epidemiologia , Estudos Transversais , Feminino , Humanos , Masculino , Prevalência , Fatores de Risco
5.
Huan Jing Ke Xue ; 37(11): 4133-4141, 2016 Nov 08.
Artigo em Chinês | MEDLINE | ID: mdl-29964662

RESUMO

Atmospheric particles, especially water-soluble inorganic ions are hazardous to human body. Motor vehicle exhaust is the greatest contributor to atmosphere pollution in Tianjin. In order to explore the emission characteristics of different road types, PM2.5 and PM10 from four types of roads were sampled and analyzed by water-soluble ions component analysis and Pearson correlation analysis during the period of April to May, 2015 to discover the ratio of PM2.5 to PM10 and the major pollutants. The results showed that water-soluble inorganic ions mainly existed in fine particles varying with different road types, which mainly came from secondary pollution. The quantities of secondary ions in PM2.5 were twice as high as that in PM10, which were the key components of the water-soluble inorganic ions. This was probably due to the increase of automobiles and their emissions, as well as the transmission and spreading of pollutants in the surroundings. Furthermore, water-soluble inorganic ions might exist in the forms of NaNO3, NH4Cl, NH4NO3, (NH4)2SO4, KCl, KNO3, K2SO4, MgCl2, CaCl2, etc. K+, Mg2+, Na+and Ca2+ showed high homology and the contributors of PM2.5 and PM10 were mainly the mixture of combustion and secondary pollutants, then followed by the mixture of re-suspended dust and traffic source.

6.
J Affect Disord ; 177: 36-41, 2015 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-25745833

RESUMO

OBJECTIVE: To assess the prevalence rate of depressive symptoms in left-behind participants aged 7-17 years and to explore the associated socio-demographic and communication factors. METHODS: Participants were 4857 left-behind children and adolescents in Chongqing. They were screened for depressive symptoms using a 27-item Children׳s Depression Inventory (CDI) and social-demographic variables were evaluated with a structured scale. Uncorrected Pearson chi-square test and logistic regression were applied to analyzing the data. RESULTS: The total prevalence rate of depressive symptoms was 24.8%. Significant difference was found in the prevalence rate of depressive symptoms among groups of different income, grade, age, left-behind subtypes, and different frequency, ways and topics of parent-child communication. We found that the risk factors for depression were absence of parents, low frequency of parent-child communication, and communication by letter or about sensitive topics. The grade group 2-3 and age group 16-17 were at a higher risk of depression than all other grade and age subgroups. The protective factors for depression were high-income, high frequency of parent-child communication, communication by telephone or about such topic as learning experience, school life, and feelings. LIMITATIONS: Self-report bias and cross-sectional nature of the sampling are major limitations of this study. CONCLUSIONS: The prevalence rate of depressive symptoms among left-behind children and adolescents in Chongqing is much higher than previously reported prevalence in other regions of China. The risk and protective factors for depression among left-behind children and adolescents are worthy of public attention.


Assuntos
Transtorno Depressivo/epidemiologia , Adolescente , Distribuição por Idade , Criança , China/epidemiologia , Comunicação , Estudos Transversais , Características da Família , Feminino , Humanos , Masculino , Relações Pais-Filho , Pais , Prevalência , Fatores de Risco , População Rural/estatística & dados numéricos , Fatores Socioeconômicos
7.
Int J Ment Health Syst ; 8(1): 1, 2014 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-24393301

RESUMO

BACKGROUND: The International Journal of Mental Health Systems (IJMHS) was launched in August 2007 and has recently been given a formal impact factor. This study uses bibliometric indicators to review the performance of the Journal against its original stated objectives and aspirations. METHODS: All articles published in IJMHS since publication commenced were included (n = 158). Selected bibliometric measures indicating Journal productivity, author affiliation, impact, geographic reach, and international collaboration were utilised. RESULTS: IJMHS published 158 articles in seven volumes over six years. Articles with three to five authors constitute the dominant authorship pattern, and authors' affiliations are varied. IJMHS has received an impact factor of 1.06 from Thomson Reuters, and the SCImago Journal Ranking shows IJMHS to be well positioned in the four categories in which it is listed, including in comparisons with well-established BMC journals that have similar scientific interests. Geographic authorship patterns show contributions from a large number of countries, including many low- and middle-income countries. DISCUSSION: Manuscript submissions from a wide range of countries, including low- and middle-income countries, are mostly from academic institutions. Authors from some geographic areas of the world are significantly under-represented. The calculation of an impact factor and encouraging rankings on the SCImago Journal Rank index are expected to lead to increased submission of high quality manuscripts. CONCLUSION: The performance of IJMHS over the first six years is promising, and the Journal is on the way to achieving the aims set out in the inaugural Editorial. IJMHS will continue to enhance its current impact through a number of new initiatives, including the introduction of thematic series and a broader range of article categories.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA