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1.
Gynecol Endocrinol ; 35(9): 807-810, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30957568

RESUMO

Kisspeptin has been shown to participate in the regulation of pituitary hormone secretion and energy metabolism. In PCOS patients, there are disorders in pituitary hormone secretion and energy metabolism. The aim of this study was to investigate the serum kisspeptin and its relationship with abnormal metabolism in PCOS. This restrospective case-control study included 73 cases with PCOS and 63 control cases. All subjects were divided into obese and nonobese groups based on BMI. The serum kisspeptin levels, Cor, DHEA-S, plasma concentrations of glucose were tested. We found that the level of kisspeptin in PCOS group was higher than it in control group. The kisspeptin levels in nonobese PCOS group increased most obviously over than the other groups. The kisspeptin levels of all the subjects were positively correlated with LH levels, negatively correlated with the glucose-AUC, the insulin-AUC, and triglyceride levels. The findings of this study suggest that kisspeptin may play an important role in ovulation disorders in PCOS patients through regulating the level of LH and it could regulate the body's energy metabolism by regulating glucose and lipid metabolism.


Assuntos
Metabolismo Energético/fisiologia , Kisspeptinas/sangue , Obesidade/complicações , Síndrome do Ovário Policístico/complicações , Síndrome do Ovário Policístico/metabolismo , Adulto , Índice de Massa Corporal , Estudos de Casos e Controles , Sulfato de Desidroepiandrosterona/sangue , Feminino , Hormônio Foliculoestimulante/sangue , Humanos , Kisspeptinas/fisiologia , Hormônio Luteinizante/sangue , Obesidade/sangue , Obesidade/metabolismo , Obesidade Abdominal/sangue , Obesidade Abdominal/complicações , Obesidade Abdominal/metabolismo , Síndrome do Ovário Policístico/sangue , Estudos Retrospectivos
2.
J Colloid Interface Sci ; 540: 602-611, 2019 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-30685683

RESUMO

HYPOTHESIS: Capillary interactions play an important role in directing colloidal assembly on fluid interfaces. Interface curvature is expected to influence not only individual particle migration on interfaces but also capillary forces between nearby particles. In drying droplets, we hypothesize that the assembly and deposition of particles bound to droplet surface are controlled by the interplay between capillary effects and evaporation-driven flow. EXPERIMENTS: Using lattice Boltzmann-Brownian dynamics (LB-BD) simulations, we modeled large-scale assembly of nanoparticles on fluid interfaces that have complex geometries and investigate the subsequent deposition upon complete evaporation. A systematic study was performed for geometrically-controlled sessile droplets whose surfaces exhibit varying curvature fields. FINDINGS: The simulations show that the particle dynamics on nonuniformly curved interfaces are anisotropic and governed by particle-pair capillary interactions and curvature-induced capillary migration. A theoretical model was developed to predict the capillarity-induced assembly. Using the curved surface as a template, drying droplets with surface-bound particles deposit distinct patterns as a result of the competition between the capillary effects and evaporation-induced convection. These findings could provide new opportunities in the directed assembly and deposition of colloidal particles with potential applications in fabricating functional materials from nanoscale building blocks.

3.
Hum Reprod ; 33(10): 1960-1967, 2018 10 01.
Artigo em Inglês | MEDLINE | ID: mdl-30189091

RESUMO

STUDY QUESTION: Is physical activity associated with incident early menopause? SUMMARY ANSWER: Physical activity is not associated with incident early menopause. WHAT IS KNOWN ALREADY: Lifestyle factors such as physical activity may influence menopause timing, but results from prior research are inconsistent. STUDY DESIGN, SIZE, DURATION: We evaluated the association between physical activity and the occurrence of early natural menopause in a prospective cohort study, the Nurses' Health Study II. Women were followed prospectively from 1989 to 2011. PARTICIPANTS/MATERIALS, SETTING, METHODS: Our analysis included 107 275 women who were premenopausal at baseline. Menopause status was self-reported biennially. Time per week participating in specific activities was reported approximately every 4 years and used to calculate metabolic task hours per week (MET h/week). We used Cox proportional hazards model to evaluate the association between physical activity and incidence of natural menopause before age 45 years while controlling for potential confounding factors. MAIN RESULTS AND THE ROLE OF CHANCE: There were 2 786 study members who experienced menopause before the age of 45. After adjustment for age, smoking and other factors, we observed no association between adulthood physical activity and early menopause. For example, compared to women reporting <3 MET h/week, the hazard ratio for women in the highest category (≥42 MET h/week) of cumulatively-averaged total physical activity was 0.89 (95% confidence interval: 0.76-1.04; P-trend: 0.26). Neither moderate nor strenuous activity in adolescence and young adulthood were related to risk. The relation of physical activity and early menopause did not vary across strata of body mass index or smoking status. LIMITATIONS, REASONS FOR CAUTION: Physical activity and menopausal status were self-reported, but repeated assessment of physical activity and prospective report of menopause status likely reduce the potential for non-differential misclassification. While the majority of our study participants were white, it is unlikely that the physiological relation of activity and early menopause varies by ethnicity. WIDER IMPLICATIONS OF THE FINDINGS: Findings from our large prospective study do not support an important association between physical activity and early menopause. STUDY FUNDING/COMPETING INTEREST(S): This project was supported by UM1CA176726 and R01HD078517 from the National Institutes of Health, Department of Health and Human Services. No competing interests are declared. TRIAL REGISTRATION NUMBER: N/A.


Assuntos
Exercício Físico/fisiologia , Menopausa Precoce/fisiologia , Adulto , Feminino , Humanos , Estudos Longitudinais , Modelos de Riscos Proporcionais , Estudos Prospectivos , Fatores de Risco , Autorrelato , Adulto Jovem
4.
PLoS One ; 13(8): e0201350, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30071040

RESUMO

Phosphorus (P) is arguably more limiting than nitrogen for forest ecosystems being free of disturbances for lengthy time periods. The elucidation of multivariate relationships between foliar P and its primary drivers for dominant species is an urgent issue and formidable challenge for ecologists. Our goal was to evaluate the effects of primary drivers on foliar P of Quercus wutaishanica, the dominant species in broadleaved deciduous forest at the Loess Plateau, China. We sampled the leaves of 90 Q. wutaishanica individuals across broad climate and soil nutrient gradients at the Loess Plateau, China, and employed structural equation models (SEM) to evaluate multiple causal pathways and the relative importance of the drivers for foliar P per unit mass (Pmass) and per unit area (Parea). Our SEMs explained 73% and 81% of the variations in Pmass and Parea, respectively. Pmass was negatively correlated to leaf mass per area, positively correlated to leaf area, and increased with mean annual precipitation and total soil potassium. Parea was positively correlated to leaf mass per area, leaf dry weight, and increased significantly with total soil potassium. Our results demonstrated that leaf P content of Q. wutaishanica increased with total soil potassium in the Loess Plateau accordingly.


Assuntos
Modelos Biológicos , Fósforo/metabolismo , Folhas de Planta/metabolismo , Potássio/metabolismo , Quercus/crescimento & desenvolvimento , Solo , Fósforo/química , Potássio/química
5.
Langmuir ; 34(25): 7445-7454, 2018 06 26.
Artigo em Inglês | MEDLINE | ID: mdl-29856637

RESUMO

We report on the use of electrospray atomization to deliver nanoparticles and surfactant directly to the surface of sessile droplets. The particles delivered to the target droplet remained adsorbed at its interface since they arrived solvent-free. Upon complete evaporation, the interface of the target drop was mapped to the underlying substrate, forming a nanoparticle deposit. The use of electrospray permitted the exploration of the interfacial particle transport and the role of surfactants in governing particle motion and deposit structure. When no surfactant was present in the sprayed solution, there was no observable convection of the interfacial particles. When Tween 80, a high-molecular-weight surfactant, was added to the sprayed solution, the surface flow was similarly suppressed. Only when small surfactants (e.g., sodium dodecyl sulfate) were present in the sprayed solution was Marangoni flow, directed toward the droplet apex, induced at the interface. This flow drove the interfacial particles to the apex of the target droplet, creating a particle-dense region at the center of the final deposit. We found that small surfactants were capable of desorbing from the interface at a sufficiently high rate relative to the evaporation time scale of the target droplet. Once inside the drop, the desorbed surfactant was convected to the contact line where it accumulated, inducing a surface tension gradient and a solutal Marangoni flow. Numerical modeling using the lattice Boltzmann-Brownian dynamics method confirmed this mechanism of particle transport and its relationship to deposit structure. The use of sacrificial targets combined with electrospray may provide a unique capability for building colloidal monolayers with organized structure in a scalable way.

6.
Langmuir ; 33(23): 5734-5744, 2017 06 13.
Artigo em Inglês | MEDLINE | ID: mdl-28548503

RESUMO

Evaporation-induced assembly of nanoparticles in a drying droplet is of great importance in many engineering applications, including printing, coating, and thin film processing. The investigation of particle dynamics in evaporating droplets can provide fundamental hydrodynamic insight for revealing the processing-structure relationship in the particle self-organization induced by solvent evaporation. We develop a free-energy-based multiphase lattice Boltzmann method coupled with Brownian dynamics to simulate evaporating colloidal droplets on solid substrates with specified wetting properties. The influence of interface-bound nanoparticles on the surface tension and evaporation of a flat liquid-vapor interface is first quantified. The results indicate that the particles at the interface reduce surface tension and enhance evaporation flux. For evaporating particle-covered droplets on substrates with different wetting properties, we characterize the increase of evaporate rate via measuring droplet volume. We find that droplet evaporation is determined by the number density and circumferential distribution of interfacial particles. We further correlate particle dynamics and assembly to the evaporation-induced convection in the bulk and on the surface of droplet. Finally, we observe distinct final deposits from evaporating colloidal droplets with bulk-dispersed and interface-bound particles. In addition, the deposit pattern is also influenced by the equilibrium contact angle of droplet.

7.
J Pineal Res ; 61(2): 241-50, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27159133

RESUMO

Traumatic brain injury (TBI) initiates a complex cascade of neurochemical and signaling changes that leads to neuronal apoptosis, which contributes to poor outcomes for patients with TBI. The neuron-specific K(+) -Cl(-) cotransporter-2 (KCC2), the principal Cl(-) extruder in adult neurons, plays an important role in Cl(-) homeostasis and neuronal function. This present study was designed to investigate the expression pattern of KCC2 following TBI and to evaluate whether or not melatonin is able to prevent neuronal apoptosis by modulating KCC2 expression in a Sprague Dawley rat controlled cortical impact model of TBI. The time course study showed decreased mRNA and protein expression of KCC2 in the ipsilateral peri-core parietal cortex after TBI. Double immunofluorescence staining demonstrated that KCC2 is located in the plasma membrane of neurons. In addition, melatonin (10 mg/kg) was injected intraperitoneally at 5 minutes and repeated at 1, 2, 3, and 4 hours after brain trauma, and brain samples were extracted 24 hours after TBI. Compared to the vehicle group, melatonin treatment altered the down-regulation of KCC2 expression in both mRNA and protein levels after TBI. Also, melatonin treatment increased the protein levels of brain-derived neurotrophic factor (BDNF) and phosphorylated extracellular signal-regulated kinase (p-ERK). Simultaneously, melatonin administration ameliorated cortical neuronal apoptosis, reduced brain edema, and attenuated neurological deficits after TBI. In conclusion, our findings suggested that melatonin restores KCC2 expression, inhibits neuronal apoptosis and attenuates secondary brain injury after TBI, partially through activation of BDNF/ERK pathway.


Assuntos
Apoptose/efeitos dos fármacos , Lesões Encefálicas Traumáticas/metabolismo , Melatonina/farmacologia , Neurônios/metabolismo , Simportadores/biossíntese , Regulação para Cima/efeitos dos fármacos , Animais , Lesões Encefálicas Traumáticas/patologia , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Masculino , Neurônios/patologia , Ratos , Ratos Sprague-Dawley
8.
Sci Rep ; 6: 26845, 2016 05 27.
Artigo em Inglês | MEDLINE | ID: mdl-27228948

RESUMO

Most terrestrial ecosystems are nitrogen (N)-limited. The elucidation of the multivariate relationships among environmental drivers, leaf morphological traits, and foliar N of dominant species which are critical to the functioning of forests remains a critical challenge for ecologists. We sampled leaves of Quercus wutaishanica across a broad natural gradient in the Loess Plateau, China, and employed structural equation modelling to evaluate the causal pathways and the relative importance of drivers of the foliar N per unit area (Narea) and per unit mass (Nmass). We found that (1) Nmass and Narea were primarily affected by leaf morphological traits instead of environmental variables and that leaf morphological traits accounted for most of their variations; (2) the total soil potassium and phosphorus and mean annual precipitation had different effects on Nmass and Narea via different pathways and path coefficients, whereas the mean annual temperature and total soil N had non-significant effects on Nmass and Narea. Our results demonstrated that variations in Nmass and Narea within Quercus wutaishanica were strongly linked to their leaf morphological traits and that the leaf N was also influenced by mean annual precipitation and soil phosphorus and potassium instead of soil N in the Loess Plateau, China.

9.
Mol Neurobiol ; 53(4): 2141-51, 2016 May.
Artigo em Inglês | MEDLINE | ID: mdl-25941074

RESUMO

The K(+)-Cl(-) cotransporter-2 (KCC2) is a well-known member of the electroneutral cation-chloride cotransporters with a restricted expression pattern to neurons. This transmembrane protein mediates the efflux of Cl(-) out of neurons and exerts a critical role in inhibitory γ-aminobutyric acidergic (GABAergic) and glycinergic neurotransmission. Moreover, KCC2 participates in the regulation of various physiological processes of neurons, including cell migration, dendritic outgrowth, spine morphology, and dendritic synaptogenesis. It is important to note that down-regulation of KCC2 is associated with the pathogenesis of multiple neurological diseases, which is of particular relevance to acute central nervous system (CNS) injury. In this review, we aim to survey the pathogenic significance of KCC2 down-regulation under the condition of acute CNS injuries. We propose that further elucidation of the molecular mechanisms regarding KCC2 down-regulation after acute CNS injuries is necessary because of potential promising avenues for prevention and treatment of acute CNS injury.


Assuntos
Sistema Nervoso Central/lesões , Cloretos/metabolismo , Homeostase , Terapia de Alvo Molecular , Simportadores/metabolismo , Traumatismos do Sistema Nervoso/metabolismo , Animais , Sistema Nervoso Central/patologia , Humanos , Modelos Biológicos , Simportadores/química , Traumatismos do Sistema Nervoso/patologia
10.
Mol Neurobiol ; 53(1): 18-27, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25399954

RESUMO

Trichostatin A (TSA), a pan-histone deacetylase inhibitor, exerts multiple neuroprotective properties. This study aims to examine whether TSA could enhance autophagy, thereby reduce neuronal apoptosis and ultimately attenuate early brain injury (EBI) following subarachnoid hemorrhage (SAH). SAH was performed through endovascular perforation method, and mortality, neurological score, and brain water content were evaluated at 24 h after surgery. Western blot were used for quantification of acetylated histone H3, LC3-II, LC3-I, Beclin-1, cytochrome c, Bax, and cleaved caspase-3 expression. Immunofluorescence was performed for colocalization of Beclin-1 and neuronal nuclei (NeuN). Apoptotic cell death of neurons was quantified with double staining of terminal deoxynucleotidyl transferase-mediated uridine 5'-triphosphate-biotin nick end-labeling (TUNEL) and NeuN. The autophagy inhibitor 3-methyladenine (3-MA) was used to manipulate the proposed pathway. Our results demonstrated that TSA reduced brain edema and alleviated neurological deficits at 24 h after SAH. TSA significantly increased acetylated histone H3, the LC3-II/LC3-I ratio, and Beclin-1 while decreased Bax and cleaved caspase-3 in the cortex. Beclin-1 and NeuN, TUNEL, and NeuN, respectively, were colocalized in cortical cells. Neuronal apoptosis in the ipsilateral basal cortex was significantly inhibited after TSA treatment. Conversely, 3-MA reversed the beneficial effects of TSA. These results proposed that TSA administration enhanced autophagy, which contributes to alleviation of neuronal apoptosis, improvement of neurological function, and attenuation of EBI following SAH.


Assuntos
Apoptose/efeitos dos fármacos , Autofagia/efeitos dos fármacos , Inibidores de Histona Desacetilases/farmacologia , Ácidos Hidroxâmicos/farmacologia , Neurônios/efeitos dos fármacos , Hemorragia Subaracnóidea/tratamento farmacológico , Animais , Lesões Encefálicas/tratamento farmacológico , Modelos Animais de Doenças , Masculino , Neurônios/metabolismo , Ratos Sprague-Dawley , Hemorragia Subaracnóidea/metabolismo , Hemorragia Subaracnóidea/patologia
11.
Mol Med Rep ; 13(1): 13-20, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26572258

RESUMO

Traumatic brain injury (TBI) leads to permanent neurological impairment, and methylene blue (MB) exerts central nervous system neuroprotective effects. However, only one previous study has investigated the effectiveness of MB in a controlled cortical impact injury model of TBI. In addition, the specific mechanisms underlying the effect of MB against TBI remain to be elucidated. Therefore, the present study investigated the neuroprotective effect of MB on TBI and the possible mechanisms involved. In a mouse model of TBI, the animals were randomly divided into sham, vehicle (normal saline) or MB groups. The treatment time­points were 24 and 72 h (acute phase of TBI), and 14 days (chronic phase of TBI) post­TBI. The brain water content (BWC), and levels of neuronal death, and autophagy were determined during the acute phase, and neurological deficit, injury volume and microglial activation were assessed at all time­points. The injured hemisphere BWC was significantly increased 24 h post­TBI, and this was attenuated following treatment with MB. There was a significantly higher number of surviving neurons in the MB group, compared with the Vehicle group at 24 and 72 h post­TBI. In the acute phase, the MB­treated animals exhibited significantly upregulated expression of Beclin 1 and increased LC3­II to LC3­I ratios, compared with the vehicle group, indicating an increased rate of autophagy. Neurological functional deficits, measured using the modified neurological severity score, were significantly lower in the acute phase in the MB­treated animals and cerebral lesion volumes in the MB­treated animals were significantly lower, compared with the other groups at all time­points. Microglia were activated 24 h after TBI, peaked at 72 h and persisted until 14 days after TBI. Although the number of Iba­1­positive cells in the vehicle and MB groups 24 h post­TBI were not significantly different, marked microglial inhibition was observed in the MB group 72 h and 14 days after ­TBI. These results indicated that MB exerts a neuroprotective effect by increasing autophagy, decreasing brain edema and inhibiting microglial activation.


Assuntos
Autofagia , Lesões Encefálicas/tratamento farmacológico , Lesões Encefálicas/patologia , Azul de Metileno/uso terapêutico , Microglia/patologia , Fármacos Neuroprotetores/uso terapêutico , Animais , Autofagia/efeitos dos fármacos , Western Blotting , Edema Encefálico/complicações , Edema Encefálico/tratamento farmacológico , Edema Encefálico/fisiopatologia , Lesões Encefálicas/complicações , Lesões Encefálicas/fisiopatologia , Masculino , Azul de Metileno/farmacologia , Camundongos Endogâmicos ICR , Microglia/efeitos dos fármacos , Microglia/metabolismo , Neurônios/efeitos dos fármacos , Neurônios/patologia , Fármacos Neuroprotetores/farmacologia , Resultado do Tratamento
12.
Sci Rep ; 5: 12713, 2015 Aug 03.
Artigo em Inglês | MEDLINE | ID: mdl-26234583

RESUMO

Colorectal cancer (CRC) is one of the most frequent malignant neoplasms worldwide. Up to now, no biomarker has been used to predict the prognosis and surveillance of patients with CRC. Recently, the association between osteopontin (OPN) overexpression and the prognosis of CRC was investigated widely, but the results were inconsistent. Therefore, the aim of present meta-analysis was to assess the prognostic effect of osteopontin in patients with CRC. PubMed, EMBASE, Web of Science, Scopus and Chinese Medical Database were systematically searched. A total of 15 studies containing 1698 patients were included in our meta-analysis. The pooled data of studies showed that high OPN expression was significantly associated with high tumor grades (OR = 2.24, 95% CI 1.55-3.23), lymph node metastasis (OR = 2.36, 95% CI 1.71-3.26) and tumor distant metastasis (OR = 2.38, 95% CI 1.01-5.60). Moreover, high OPN expression was significantly associated with the 2-year (HR 1.97, 95% CI 1.30-3.00), 3-year (HR 1.82, 95% CI 1.24-2.68), 5 year (HR 1.53, 95% CI 1.28-1.82) survival rates and overall survival (OS, HR 1.70, 95% CI 1.12-2.60), respectively. These results indicated that OPN could serve as a prognostic biomarker and as a potential therapeutic target for CRC.


Assuntos
Neoplasias Colorretais/mortalidade , Neoplasias Colorretais/patologia , Osteopontina/metabolismo , Biomarcadores Tumorais/genética , Biomarcadores Tumorais/metabolismo , Neoplasias Colorretais/metabolismo , Expressão Gênica , Humanos , Osteopontina/genética , Prognóstico , Taxa de Sobrevida
13.
Hum Pathol ; 46(6): 843-9, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25841304

RESUMO

The objective of the present study was to determine whether 2 proteins (nuclear erythroid 2-related factor 2 [Nrf2] and p62) can be used as the biomarkers for prognosis of gliomas. The glioma samples were from 75 glioma patients after surgery. The expression of Nrf2 and p62 in 75 samples was detected with immunohistochemical staining. The correlation between immunohistochemical results and clinicopathological parameters or prognosis was analyzed. The results indicated that high Nrf2 expression was detected in 61.3% of glioma tissue samples and was significantly correlated with age (P = .006), tumor grade (P = .002), and onset time (P = .019). The overall survival (OS) and disease-free survival (DFS) in patients with high Nrf2 expression were significantly shorter than those in patients with low Nrf2 expression (P < .001). High p62 expression was detected in 65.3% of glioma tissue samples, and p62 expression was correlated significantly with the tumor grade (P < .001) and 1-year survival (P = .024). OS and DFS in patients with high p62 expression were significantly shorter than those in patients with low p62 expression (P < .001). Spearman rank correlation test revealed a significant positive relation between Nrf2 and p62 expressions (r = 0.515, P < .001). Multivariate regression analysis showed that p62 expression and age were the significant independent prognostic factors for DFS (P < .05) and tumor grade and p62 expression were independent prognostic parameters for OS (P < .01 or P < .05). These findings indicated that p62 may be used as the prognostic biomarker in patients with gliomas.


Assuntos
Neoplasias Encefálicas/metabolismo , Glioma/metabolismo , Fator 2 Relacionado a NF-E2/metabolismo , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Biomarcadores/análise , Neoplasias Encefálicas/patologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Prognóstico , Adulto Jovem
14.
PLoS One ; 10(4): e0123139, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25849554

RESUMO

The objective of this study was to identify new viral biomarkers associated with acute on chronic liver failure (ACLF) by complete genomic sequencing of HBV. Hepatitis B virus mutations associated with ACLF were screened by Illumina high-throughput sequencing in twelve ACLF cases and twelve age-matched mild chronic hepatitis B patients, which were validated in 438 chronic hepatitis B patients (80 asymptomatic carriers, 152 mild chronic hepatitis B patients, 102 severe chronic hepatitis B patients and 104 ACLF patients) by direct sequencing. The results of Illumina sequencing showed that the mutations at 7 sites (T216C, G285A, A1846T, G1896A, C1913A/G, A2159G, and A2189C) of 12 ACLF patients were significantly higher than those of 12 controls. In the validation cohorts, a significantly higher ratio of genotype B to C was found in patients with ACLF than in patients with non-ACLF. Multivariate analysis showed that T216C, G1896A, C1913A/G and A2159G/C were independent risk factors for ACLF. C216 in any combination, A/G1913 in any combination, and G/C2159 in any combination had high specificity for ACLF. In summary, T216C and A2159G/C mutations were novel factors independently associated with ACLF. Combined mutations in hepatitis B cases could play important roles in ACLF development.


Assuntos
Insuficiência Hepática Crônica Agudizada/genética , Antígenos do Núcleo do Vírus da Hepatite B/genética , Antígenos de Superfície da Hepatite B/genética , Vírus da Hepatite B/genética , Hepatite B/genética , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Mutação Puntual/genética , Insuficiência Hepática Crônica Agudizada/virologia , Adulto , Mapeamento Cromossômico , DNA Viral/genética , Feminino , Genômica/métodos , Genótipo , Hepatite B/virologia , Humanos , Masculino , Regiões Promotoras Genéticas/genética
15.
Tumour Biol ; 36(1): 429-36, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25266800

RESUMO

So far, several studies on the association between osteopontin (OPN) expression and glioma have been performed, but the conclusion still was not clear. The aim of the present meta-analysis was to determine the relationship between OPN expression and prognosis of patients with glioma. The electronic database was searched for articles on the association between OPN expression and glioma until 31 January 2014. Odds ratios (OR) and the relative risks with 95 % CI were utilized to analyze the qualitative data in retrospective studies and prospective studies, respectively. The standardized mean difference and the corresponding 95 % CI were used for analyzing the studies with quantitative data. Heterogeneity of all included studies was assessed using Cochrane's Q test and I (2) measurement. The publication bias was examined by the Egger test. Sixteen cohort studies (854 patients) on OPN expression and gliomas prognosis were included in the present meta-analysis. It was found that OPN expression was significantly higher in patients with high-grade glioma than in patients with low-grade glioma (χ (2) = 8.38, I (2) = 16.6 %, P = 0.300), and the expression of OPN increased with glioma grade. The combined data showed the correlation between high OPN expression and tumor reoccurrence (OR = 18.61, 95 % CI = 6.34-54.67, P = 0.405). In addition, the results of the pooled analysis indicated that OPN expression was significantly related to overall survival (HR = 1.83; 95 % CI = 1.36-2.46). In conclusion, OPN may be a biomarker for predicting the prognosis of gliomas.


Assuntos
Biomarcadores Tumorais/metabolismo , Neoplasias Encefálicas/metabolismo , Glioma/metabolismo , Osteopontina/metabolismo , Neoplasias Encefálicas/mortalidade , Neoplasias Encefálicas/patologia , Glioma/mortalidade , Glioma/patologia , Humanos , Prognóstico , Análise de Sobrevida , Carga Tumoral
16.
Int J Clin Exp Pathol ; 8(11): 15369-74, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26823896

RESUMO

Composite pheochromocytoma-ganglioneuroma is extremely rare. We described two cases of composite pheochromocytomas in the adrenal medullar. Case 1 was a 70-year-old male presenting with lower abdominal pain and normal blood electrolytes. Case 2 was a 48-year-old female with palpitation and back tenderness. Biochemical investigations showed hypocalcium, hypokalemia and high level of vma. The histological images and the immunohistochemical staining demonstrated the two cases composed of pheochromocytoma and ganglioneuromoma components. Ganglioneuroma component in case 2 accounted for more proportion than that in case 1. We speculated that the varied clinical symptoms were related with the diverse proportions in composite pheochromocytome-ganglioneuroma.


Assuntos
Neoplasias das Glândulas Suprarrenais/patologia , Medula Suprarrenal/patologia , Ganglioneuroma/patologia , Neoplasias Complexas Mistas , Feocromocitoma/patologia , Neoplasias das Glândulas Suprarrenais/química , Neoplasias das Glândulas Suprarrenais/complicações , Neoplasias das Glândulas Suprarrenais/cirurgia , Medula Suprarrenal/química , Medula Suprarrenal/cirurgia , Adrenalectomia , Idoso , Biomarcadores Tumorais/análise , Biópsia , Feminino , Ganglioneuroma/química , Ganglioneuroma/complicações , Ganglioneuroma/cirurgia , Humanos , Hipocalcemia/etiologia , Hipopotassemia/etiologia , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Feocromocitoma/química , Feocromocitoma/complicações , Feocromocitoma/cirurgia , Tomografia Computadorizada por Raios X
17.
Int J Clin Exp Pathol ; 7(12): 9081-5, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25674292

RESUMO

Primary intradural extraskeletal Ewing sarcoma is a very rare form of malignant neoplasm. Only few cases have been reported on the literature. Here, we report a case of a 14-year-old boy who had a chief complaint of pain and tingling in the right lower limb. The patient initially seemed to have a giant nerve sheath tumor but was eventually diagnosed with intradural extraskeletal Ewing sarcoma arising from the nerve roots of the cauda equine. The literature with regard to primary spinal intradural extraskeletal Ewing sarcoma is reviewed.


Assuntos
Cauda Equina/patologia , Dura-Máter/patologia , Neoplasias da Bainha Neural/patologia , Sarcoma de Ewing/patologia , Neoplasias da Medula Espinal/patologia , Adolescente , Biópsia , Cauda Equina/cirurgia , Diagnóstico Diferencial , Dura-Máter/cirurgia , Humanos , Imagem por Ressonância Magnética , Masculino , Valor Preditivo dos Testes , Sarcoma de Ewing/complicações , Sarcoma de Ewing/cirurgia , Neoplasias da Medula Espinal/complicações , Neoplasias da Medula Espinal/cirurgia , Resultado do Tratamento , Carga Tumoral
18.
PLoS One ; 8(10): e75371, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24116040

RESUMO

OBJECTIVE: The results of studies on the relation between Mannose-binding lectin gene (mbl2) polymorphism and HBV infection were contradictory and inconclusive. In order to shed a light on these inconsistent findings and to clarify the role of mbl2 polymorphisms in susceptibility or progression of chronic hepatitis B (CHB), a meta-analysis was performed. METHODS: PubMed and Embase were searched for available articles. A meta-analysis was performed to examine the association between mbl2 polymorphisms and chronicity or progression of hepatitis B infection. Odds ratio (OR) and its 95% confidence interval (CI) served as indexes. RESULTS: A total of 17 eligible studies were involved, including 2151 healthy controls (HC), 1293 spontaneous recovered (SR) patients with acute infection, 2337 cases with chronic hepatitis B (CHB) and 554 cases with progressive hepatitis B. There was no evidence of significant association between mbl2 exon1 polymorphisms and CHB risk in any genetic model or pairwise comparisons when compared with HC group or SR group. In the stratified analysis of ethnic groups, also no obvious relation between mbl2 polymorphism and CHB risk was identified. There was still no significant association between the complete mbl2 genotypic profile (including both the exon1 and the promoter gene) polymorphisms and CHB risk, as compared with SR group. However, it was found that there was an association between the mbl2 AO/OO genotype and severe hepatitis B (SHB) or liver cirrhosis (LC) (LC vs. HC:OR=3.66, 95%CI, 2.38-5.63; SHB vs. HC, OR=3.88, 95%CI, 2.26-6.64), but there was no relationship between the mbl2 AO/OO genotype and hepatocellular carcinoma (HCC) (OR=1.26, 95%CI, 0.82-1.94). CONCLUSION: The present meta-analysis indicated that mbl2 exon1 polymorphisms might not significantly associate with chronicity of HBV infection, but might be significantly related to the progressive HBV such as SHB and LC.


Assuntos
Hepatite B/genética , Lectina de Ligação a Manose/genética , Polimorfismo Genético , Progressão da Doença , Estudos de Associação Genética , Predisposição Genética para Doença , Humanos , Regiões Promotoras Genéticas
19.
J Int Med Res ; 41(3): 559-67, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23669295

RESUMO

OBJECTIVE: This meta-analysis determined the relationship between polymorphisms of the cytotoxic T-lymphocyte-associated protein 4 (CTLA4) gene and hepatitis B virus (HBV) clearance in chronic hepatitis B. METHODS: Published studies reporting associations between CTLA4 gene +49A/G polymorphisms and chronic HBV infection were reviewed. Odds ratio (OR) and 95% confidence interval (CI) were calculated to assess the risk of persistent HBV according to genotype. RESULTS: Six studies, involving 1076 chronic HBV patients and 1294 controls, were included. The risk of persistent HBV in patients with a +49 GG/AG genotype decreased significantly compared with the AA genotype (OR 0.65; 95% CI 0.52, 0.82). The variant G allele was negatively associated with chronic HBV infection versus the A allele (OR 0.77; 95% CI 0.68, 0.88). When stratifying by type of study control, a significantly decreased risk was associated with CTLA4+49 variant genotypes (AG and GG) in both spontaneous recovery control group and healthy control group. CONCLUSIONS: Findings of this meta-analysis suggest that A at position +49 of the CTLA4 gene may significantly increase the risk of persistent HBV infection, whereas G at position +49 may positively influence virus clearance.


Assuntos
Antígeno CTLA-4/genética , Predisposição Genética para Doença , Hepatite B Crônica/genética , Polimorfismo de Nucleotídeo Único , Linfócitos T Citotóxicos/metabolismo , Alelos , Antígeno CTLA-4/imunologia , Estudos de Casos e Controles , Expressão Gênica , Frequência do Gene , Vírus da Hepatite B/fisiologia , Hepatite B Crônica/imunologia , Hepatite B Crônica/virologia , Humanos , Razão de Chances , Risco , Linfócitos T Citotóxicos/imunologia , Linfócitos T Citotóxicos/virologia , Replicação Viral
20.
J Hazard Mater ; 158(1): 43-51, 2008 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-18314268

RESUMO

The adsorption kinetics of a cationic dye, methylene blue (MB), onto the silica nano-sheets derived from vermiculite via acid leaching was investigated in aqueous solution in a batch system with respect to contact time, initial dye concentration, pH, and temperature. Experimental results have shown that increasing initial dye concentration favors the adsorption while the acidic pH and temperature go against the adsorption. Experimental data related to the adsorption of MB on the silica nano-sheets under different conditions were applied to the pseudo-first-order equation, the pseudo-second-order equation and the intraparticle diffusion equation, and the rate constants of first-order adsorption (k(1)), the rate constants of second-order adsorption (k(2)) and intraparticle diffusion rate constants (k(int)) were calculated, respectively. The experimental data fitted very well the pseudo-second-order kinetic model. The activation energy of system (E(a)) was calculated as 3.42 kJ/mol. The thermodynamics parameters of activation such as Gibbs free energy, enthalpy, entropy were also evaluated and found that DeltaG*, DeltaH*, and DeltaS* are 65.95 (71.63, 77.45)kJ/mol, 0.984 (0.776, 0.568)kJ/mol, and -0.222 (-0.223, -0.224)kJ/(Kmol) at 20 (45, 70) degrees C, respectively. The desorption of the dye on the silica nano-sheets using ethanol was also investigated primarily.


Assuntos
Silicatos de Alumínio/química , Azul de Metileno/isolamento & purificação , Nanoestruturas/química , Dióxido de Silício/química , Eliminação de Resíduos Líquidos/métodos , Adsorção , Difusão , Concentração de Íons de Hidrogênio , Cinética , Modelos Químicos , Temperatura , Termodinâmica
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