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1.
Artigo em Inglês | MEDLINE | ID: mdl-33161490

RESUMO

Broad-spectrum antibodies can effectively recognize substances with similar structures and have broad application prospects in field rapid detection. In this study, broad-spectrum antibodies (Abs) against organophosphorus pesticides (OPs) were used as sensitive recognition elements, which could effectively recognize most OPs. Gold nanoparticles (AuNPs) have good biocompatibility. It combined with Abs to form a gold-labeled probe (AuNPs-Abs), which enhances the effective binding of antibodies to nanomaterials. Prussian blue (PB) was added to electrodeposition solution to enhance the conductivity, resulting in superior electrochemical performance. The AuNP-Abs-PB composite film was prepared by electrodeposition on the electrode surface to improve the anti-interference ability and stability of the immunosensor. Under the optimal experimental conditions, the immunosensor had a wide detection range (IC20-IC80: 1.82 × 10-3-3.29 × 104 ng/mL) and high sensitivity. Most importantly, it was simple to be prepared and could be used to detect multiple OPs.

2.
JMIR Ment Health ; 7(7): e15797, 2020 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-32347799

RESUMO

BACKGROUND: Prior research has demonstrated the efficacy of internet-based cognitive behavioral therapy (ICBT) for social anxiety disorder (SAD). However, it is unclear how shame influences the efficacy of this treatment. OBJECTIVE: This study aimed to investigate the role shame played in the ICBT treatment process for participants with SAD. METHODS: A total of 104 Chinese participants (73 females; age: mean 24.92, SD 4.59 years) were randomly assigned to self-help ICBT, guided ICBT, or wait list control groups. For the guided ICBT group, half of the participants were assigned to the group at a time due to resource constraints. This led to a time difference among the three groups. Participants were assessed before and immediately after the intervention using the Social Interaction Anxiety Scale (SIAS), Social Phobia Scale (SPS), and Experience of Shame Scale (ESS). RESULTS: Participants' social anxiety symptoms (self-help: differences between pre- and posttreatment SIAS=-12.71; Cohen d=1.01; 95% CI 9.08 to 16.32; P<.001 and differences between pre- and posttreatment SPS=11.13; Cohen d=0.89; 95% CI 6.98 to 15.28; P<.001; guided: SIAS=19.45; Cohen d=1.20; 95% CI 14.67 to 24.24; P<.001 and SPS=13.45; Cohen d=0.96; 95% CI 8.26 to 18.64; P<.001) and shame proneness (self-help: differences between pre- and posttreatment ESS=7.34; Cohen d=0.75; 95% CI 3.99 to 10.69; P<.001 and guided: differences between pre- and posttreatment ESS=9.97; Cohen d=0.88; 95% CI 5.36 to 14.57; P<.001) in both the self-help and guided ICBT groups reduced significantly after treatment, with no significant differences between the two intervention groups. Across all the ICBT sessions, the only significant predictors of reductions in shame proneness were the average number of words participants wrote in the exposure module (ß=.222; SE 0.175; t96=2.317; P=.02) and gender (ß=-.33; SE 0.002; t77=-3.13; P=.002). We also found a mediation effect, wherein reductions in shame fully mediated the relationship between the average number of words participants wrote in the exposure module and reductions in social anxiety symptoms (SIAS: ß=-.0049; SE 0.0016; 95% CI -0.0085 to -0.0019 and SPS: ß=-.0039; SE 0.0015; 95% CI -0.0075 to -0.0012). CONCLUSIONS: The findings of this study suggest that participants' engagement in the exposure module in ICBT alleviates social anxiety symptoms by reducing the levels of shame proneness. Our study provides a new perspective for understanding the role of shame in the treatment of social anxiety. The possible mechanisms of the mediation effect and clinical implications are discussed. TRIAL REGISTRATION: Chinese Clinical Trial Registry ChiCTR1900021952; http://www.chictr.org.cn/showproj.aspx?proj=36977.

3.
Sci Total Environ ; 712: 136410, 2020 Apr 10.
Artigo em Inglês | MEDLINE | ID: mdl-32050375

RESUMO

In order to solve the problem that the sensor cannot be reused due to the passivation of the electrode surface, a refreshable electrochemical aptasensor based on a hydrophobic electrode and a magnetic nanocomposite had been developed. Therein, the hydrophobic electrode was formed by modifying a screen-printed carbon electrode (SPCE) with polydimethylsiloxane (PDMS), which could avoid adsorption of molecules on the electrode surface due to its hydrophobicity. Combined with aptamer (Apt), the synthesized graphene oxide-ferroferric oxide (GO-Fe3O4) was used as a magnetic catcher to capture specific organophosphorus pesticides (OPs), which could be removed to the working area of SPCE with a magnet for electrochemical detection. The performance analysis of hydrophobic electrode showed that the SPCE could be used twice. When the electrochemical signals of Apt/GO-Fe3O4 and OPs/Apt/GO-Fe3O4 were recorded using the same SPCE, the current differences between them were directly related to the concentrations of OPs. Through the contrast test between the spiked vegetable samples and the OPs standard solutions, it was found that the OPs concentrations could be qualitatively evaluated by comparing the current differences. At the same time, the characteristic of collecting target with magnetic catcher was helpful for detecting OPs with a low concentration. Therefore, the refreshable aptasensor provided a huge potential to small molecule target evaluation.

4.
Sensors (Basel) ; 19(8)2019 Apr 17.
Artigo em Inglês | MEDLINE | ID: mdl-30999637

RESUMO

The Internet of Things (IoT) has tremendous success in health care, smart city, industrial production and so on. Protected agriculture is one of the fields which has broad application prospects of IoT. Protected agriculture is a mode of highly efficient development of modern agriculture that uses artificial techniques to change climatic factors such as temperature, to create environmental conditions suitable for the growth of animals and plants. This review aims to gain insight into the state-of-the-art of IoT applications in protected agriculture and to identify the system structure and key technologies. Therefore, we completed a systematic literature review of IoT research and deployments in protected agriculture over the past 10 years and evaluated the contributions made by different academicians and organizations. Selected references were clustered into three application domains corresponding to plant management, animal farming and food/agricultural product supply traceability. Furthermore, we discussed the challenges along with future research prospects, to help new researchers of this domain understand the current research progress of IoT in protected agriculture and to propose more novel and innovative ideas in the future.


Assuntos
Agricultura/tendências , Redes de Comunicação de Computadores , Abastecimento de Alimentos , Internet/tendências , Cidades , Humanos , Tecnologia sem Fio
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