Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 14 de 14
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Zhonghua Xin Xue Guan Bing Za Zhi ; 47(10): 790-797, 2019 Oct 24.
Artigo em Chinês | MEDLINE | ID: mdl-31648461

RESUMO

Objective: To explore the value of SYNTAX revascularization index (SRI) on evaluating the long-term prognosis of coronary artery disease (CAD) patients implanted with biodegradable polymer drug-eluting stents (BP-DES) and define the best threshold of SRI for predicting all-cause mortality in these patients. Methods: Data used in this study derived from the I-LOVE-IT 2 trial (evaluate safety and effectiveness of the Tivoli DES and the Firebird DES for treatment of coronary). I-LOVE-IT 2 trial was a prospective, multicenter, randomized, assessor-blinded, non-inferiority study. A total of 1 829 patients implanted with BP-DES were divided into 3 groups, namely SRI=100% group (n=963), 50%≤SRI<100% group (n=527) and SRI<50% group (n=339). The primary endpoint was 48-month patient-oriented composite endpoint (PoCE), a composite of all-cause mortality, myocardial infarction(MI), stroke, and/or any revascularization. The secondary endpoints were components of PoCE and definite/probable stent thrombosis at 48 months. The receiver operating characteristic curve was used to investigate the best cut-off point of SRI for 48-month all-cause mortality. The Cox regression analysis was used to identify independent predictors of the all-cause death and PoCE at 48 months. Results: Incidence of PoCE at 48 months was significantly lower in SRI=100% group than patients with 50%≤SRI<100%(17.34% (167/963) vs. 22.20% (117/527), P<0.05) and SRI<50% (17.34% (167/963) vs. 24.78% (84/339), P<0.05). Comparing with SRI=100% group, the patients with 50%≤SRI<100% suffered higher rates of all MI (7.78% (41/527) vs. 4.26% (41/963), P<0.05) and target vessel MI (6.45% (34/527) vs. 4.26% (41/963), P<0.05); patients with SRI<50% had higher rates of all-cause mortality (5.90% (20/339) vs. 3.12% (30/963), P<0.05) and any revascularization (14.16% (48/339) vs. 3.12% (30/963), P<0.05). The receiver operating characteristic curve analysis showed that the SRI=65% was the best cut-off point to predict the all-cause mortality at 48 months (area under the curve was 0.58, sensitive was 0.47, specificity was 0.70). Meanwhile, SRI<65% was an independent predictor of 48-month all-cause mortality (HR=2.06, 95%CI 1.25-3.38) and PoCE (HR=1.34, 95%CI 1.09-1.66). Conclusions: SRI serves as a good index for predicting long-term prognosis and SRI<65% is an independent predictor of 48-month PoCE and all-cause mortality for CAD patients with BP-DES implantation. Meanwhile, SRI≥65% might be a reasonable threshold of incomplete revascularization.


Assuntos
Doença da Artéria Coronariana/terapia , Stents Farmacológicos , Implantes Absorvíveis , Humanos , Mortalidade , Polímeros , Prognóstico , Estudos Prospectivos , Fatores de Risco , Resultado do Tratamento
2.
Eur Rev Med Pharmacol Sci ; 23(13): 5667-5674, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31298318

RESUMO

OBJECTIVE: The aim of this study was to explore the role of microRNA-203 (miR-203) in Prostate Cancer (PCa), and to further verify its influence in PCa cell function. PATIENTS AND METHODS: The expression level of miR-203 in 55 clinical PCa cases and cell lines was detected by qRT-PCR. Then, the target gene of miR-203 in PCa cells was predicted and verified by online prediction software and Luciferase reporter gene assay, respectively. Furthermore, the role of miR-203 in PCa cell proliferation, colony formation, cell cycle and metastasis capacities was detected through a series of in vitro experiments. RESULTS: The expression of miR-203 in PCa tissues and cells was significantly reduced when compared with that of normal tissues and cells. In searching for potential downstream targets of miR-203, a regulator of G-protein signaling 17 (RGS17) entered our sight due to its active role in a variety of malignant tumors. More importantly, the negative regulation of RGS17 by miR-203 was verified by Luciferase reporter gene assay. Functional experiments demonstrated that low expression of RGS17 in PCa cells induced by up-regulation of miR-203 could significantly restrain the proliferation, invasion and migration capacities of PCa cells. CONCLUSIONS: MiR-203 served as a tumor suppressor gene in PCa. Through targeting RGS17, miR-203 significantly controlled the malignant behavior of PCa cells. Our findings revealed that miR-203/RGS17 axis might be a potential therapeutic target for the treatment of PCa.

3.
J Econ Entomol ; 108(2): 484-91, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26470159

RESUMO

A monoclonal antibody was prepared by the hybridoma technology. It reacted only with the protein of Liriomyza trifolii (Burgess) and not with that of Chromatomyia horticola Goureau or Liriomyza sativae Blanchard in indirect enzyme-linked immunosorbent assay. It was effective even after being diluted more than 8.192×10(6)-fold. The detection sensitivity of the antibody was 31.3 µg/ml under controlled conditions. Positive reaction was achieved with all laboratory-reared L. trifolii samples, including larvae, pupae, and adults. An indirect enzyme-linked immunosorbent assay system was successfully established to detect L. trifolii in the field. This antibody was successfully used to determine the L. trifolii collected in different locations, from different host plants, and in different seasons. More than 50% of leafminers collected on Brassica chinensis var chinensis, Apium graveolens (Miller) Persoon, Vigna unguiculata (L.) Walpers, Phaseolus vulgaris L., Lactuca sativa L., and Chrysanthemum coronarium (L.) Cassini ex Spach were L. trifolii, indicating that those six plant species might be the preference host plants of L. trifolii. Population of L. trifolii peaked in September, October, or November in Hangzhou, Zhejiang. These results suggest a great potential of using this McAb for precisely identifying L. trifolii and monitoring the population dynamics of L. trifolii in the field.


Assuntos
Dípteros/classificação , Dípteros/imunologia , Espécies Introduzidas , Animais , Anticorpos Monoclonais/biossíntese , Ensaio de Imunoadsorção Enzimática , Camundongos Endogâmicos BALB C
4.
Genet Mol Res ; 14(3): 10530-42, 2015 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-26400285

RESUMO

The use of appropriate reference genes is essential for the generation of accurate and biologically meaningful results from quantitative real-time PCR (qRT-PCR) analysis. However, studies have found that the expression of most commonly used reference genes is not always independent of the tissues, treatments, or developmental stages studied. geNormPlus, NormFinder, and BestKeeper, were applied and the expression stability of nine candidate genes was evaluated in different data sets during wheat grain development. Varying degrees of diversity in either single or multiple reference genes were observed among the results generated from the different computer programs, parameters, and data sets. Therefore, the reliability of identified reference genes in the flag leaf and the complete set of samples was estimated by monitoring the expression dynamics of three NAM genes (TaNAM-A1, TaNAM-B1, and TaNAM-B2). The results suggest that a single control gene identified by geNormPlus for use with the complete set of samples, and multiple reference genes selected by geNormPlus and NormFinder exclusively for the flag leaf outperformed others owing to the consistent results with previous analyses of these genes, which were normalized against a verified single control gene. Given the limit of NormFinder in gene numbers of multiple reference genes, robust quantification can be achieved by normalizing against Ta27922 or multiple reference genes chosen by geNormPlus for individual tissues.


Assuntos
Regulação da Expressão Gênica de Plantas , Genes Essenciais , Genes de Plantas , Sementes/genética , Software , Triticum/genética , Grão Comestível , Perfilação da Expressão Gênica , Regulação da Expressão Gênica no Desenvolvimento , Variação Genética , Reação em Cadeia da Polimerase em Tempo Real , Padrões de Referência , Sementes/crescimento & desenvolvimento , Triticum/crescimento & desenvolvimento
5.
Genet Mol Res ; 14(3): 8077-83, 2015 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-26214490

RESUMO

The transfer of agronomically useful genes from wild wheat species into cultivated wheat is one of the most effective approaches to improvement of wheat varieties. To evaluate the transfer of genes from Dasypyrum villosum into Triticum aestivum, wheat quality and disease resistance was evaluated in two new translocation lines, T1DL•1V#3S and T1DS•1V#3L. We examined the levels of stripe rust resistance and dough quality in the two lines, and identified and located the stripe rust resistant genes and high molecular weight glutenin subunit (HMW-GS) genes Glu-V1 of D. villosum. Compared to the Chinese Spring (CS) variety, T1DL•1V#3S plants showed moderate resistance to moderate susceptibility to the stripe rust races CYR33 and Su11-4. However, T1DS•1V#3L plants showed high resistance or immunity to these stripe rusts. The genes for resistance to stripe rust were located on 1VL of D. villosum. In comparison to CS, the dough from T1DS•1V#3L had a significantly shorter developing time (1.45 min) and stable time (1.0 min), a higher weakness in gluten strength (208.5 FU), and a lower farinograph quality index (18). T1DL•1V#3S had a significantly longer developing time (4.2 min) and stable time (5.25 min), a lower weakness in gluten strength (53 FU) and a higher farinograph quality index (78.5). We also found that T1DS•1V#3L had reduced gluten strength and dough quality compared to CS, but T1DL•1V#3S had increased gluten strength and dough quality. The results of SDS-PAGE analysis indicated that Glu-V1 of D. villosum was located on short arm 1VS and long arm 1VL. These results prove that the new translocation lines, T1DS•1V#3L and T1DS•1V#3L, have valuable stripe rust resistance and dough quality traits that will be important for improving wheat quality and resistance in future wheat breeding programs.


Assuntos
Basidiomycota/fisiologia , Resistência à Doença/genética , Farinha/normas , Genes de Plantas , Glutens/genética , Doenças das Plantas/microbiologia , Poaceae/genética , Triticum/genética , Ecótipo , Eletroforese em Gel de Poliacrilamida , Doenças das Plantas/genética , Subunidades Proteicas/genética
6.
Genet Mol Res ; 14(2): 5577-84, 2015 May 25.
Artigo em Inglês | MEDLINE | ID: mdl-26125755

RESUMO

We examined the variation in plasma levels of endothelin (ET), calcitonin gene-related peptide (CGRP), nitric oxide (NO), and malondialdehyde (MDA), as well as superoxide dismutase (SOD) activity, in acute myocardial ischemia reperfusion injury in a rabbit model. Seventy rabbits were randomly assigned into 3 groups. Open-chest surgery (OCS) was performed for all rabbits. Group A (N = 20) received sham-surgery, group B (N = 25) was the reperfusion group, and group C (N = 25) was the infarction group. At 12 h after chest clo-sure, plasma ET levels in groups B and C were clearly increased, while CGRP levels were clearly decreased, particularly in group B. At 24 h after chest closure, ET levels were higher than before OCS, while there was no significant difference between groups B and C. ET in group B was decreased, while that in group C was increased at 12 h. No significant difference in CGRP was observed between 12 and 24 h after chest closure. NO levels in groups B and C at 12 h after chest closure were significantly decreased compared to those before OCS. NO levels in group B at 24, 48, and 72 h were significantly lower than those at 12 h, while those of group C were not significantly changed after 12 h. Dynamic monitoring and comparison of plasma levels of ET, CGRP, NO, and MDA as well as SOD activity revealed that appropriate intervention of these factors may reduce reperfusion injury.


Assuntos
Peptídeo Relacionado com Gene de Calcitonina/sangue , Endotelinas/sangue , Malondialdeído/sangue , Traumatismo por Reperfusão Miocárdica/sangue , Óxido Nítrico/sangue , Animais , Modelos Animais de Doenças , Humanos , Traumatismo por Reperfusão Miocárdica/patologia , Miocárdio/patologia , Coelhos , Superóxido Dismutase/sangue
8.
Eur J Surg Oncol ; 39(8): 858-64, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-23669199

RESUMO

BACKGROUND: The preoperative predictors of microvascular invasion (MVI) in multinodular hepatocellular carcinoma (HCC) are currently unclear. METHODS: We retrospectively analyzed 266 patients who underwent potentially curative resection of multinodular HCC. MVI was diagnosed on pathological examination in 64 patients. Preoperative risk factors for MVI were identified and survival curves were analyzed. RESULTS: Patients with MVI had significantly lower overall and recurrence-free survival rates than those without MVI (overall survival, 1 year: 86% vs. 71%, 3 years: 58% vs. 16%; recurrence-free survival, 1 year: 69% vs. 12%; 3 years: 48% vs. 12%; both P < 0.001). Multivariate analysis showed that serum alpha-fetoprotein (AFP) level >400 µg/L (odds ratio [OR] = 3.732, P = 0.016), serum gamma-glutamyltransferase (GGT) level >130 U/L (OR = 19.779, P < 0.001), total tumor diameter >8 cm (OR = 5.545, P = 0.010), and tumor number >3 (OR = 11.566, P = 0.007) were independent predictors of MVI. A scoring system was constructed, and the MVI rate was significantly higher in patients with a score of ≥3 than those with a score of <3 (64.1% vs. 10.9%, P < 0.001). Overall and recurrence-free survival rates were significantly lower in patients with a score of ≥3 (both P < 0.001). CONCLUSIONS: Serum AFP level >400 µg/L, serum GGT level >130 U/L, total tumor diameter >8 cm, and tumor number >3 were preoperative predictors of MVI in patients with multinodular HCC. In patients with a high risk of MVI and well-preserved liver function, anatomic resection may be worth considering.


Assuntos
Carcinoma Hepatocelular/mortalidade , Carcinoma Hepatocelular/patologia , Neoplasias Hepáticas/mortalidade , Neoplasias Hepáticas/patologia , Fígado/irrigação sanguínea , Adulto , Idoso , Análise de Variância , Carcinoma Hepatocelular/cirurgia , Estudos de Coortes , Feminino , Hepatectomia/métodos , Hepatectomia/mortalidade , Humanos , Neoplasias Hepáticas/cirurgia , Imagem por Ressonância Magnética/métodos , Masculino , Microcirculação , Microvasos/patologia , Pessoa de Meia-Idade , Análise Multivariada , Invasividade Neoplásica/patologia , Estadiamento de Neoplasias , Razão de Chances , Valor Preditivo dos Testes , Cuidados Pré-Operatórios/métodos , Prognóstico , Estudos Retrospectivos , Medição de Risco , Análise de Sobrevida , Tomografia Computadorizada por Raios X/métodos
9.
Ophthalmic Res ; 50(1): 6-12, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23549077

RESUMO

BACKGROUND/AIMS: Stromal cell-derived factor-1 (SDF-1) has been shown to mediate a broad range of biological processes via CXCR4, once regarded as its only receptor. CXCR7 is a recently identified receptor for SDF-1. This study aimed to investigate whether the CXCR7/CXCR4/SDF-1 axis is involved in choroidal neovascularization (CNV) formation in an in vitro hypoxic model. METHODS: CXCR7 siRNA and/or CXCR4 siRNA was transfected into a hypoxic model of the choroid-retinal endothelial RF/6A cell line. CCK-8 analysis, transwell migration analysis, annexin V-FITC and propidium iodide staining, and Matrigel tube formation analysis were performed to investigate the role of CXCR4 and CXCR7 in SDF-1-induced proliferation, migration, survival and tube formation of RF/6A cells. RESULTS: CXCR4, but not CXCR7, mediates SDF-1-induced RF/6A cell migration and proliferation under hypoxic conditions, whereas CXCR7 was exclusively involved in RF/6A cell survival. In addition, CXCR7 and CXCR4 acted together to regulate RF/6A cell tube formation. CONCLUSION: The CXCR7/CXCR4/SDF-1 axis plays an important role in the formation of CNV, and may become a novel target for the treatment of CNV-associated diseases.


Assuntos
Quimiocina CXCL12/fisiologia , Neovascularização de Coroide/patologia , Células Endoteliais/fisiologia , Receptores CXCR/fisiologia , Análise de Variância , Animais , Western Blotting , Movimento Celular/fisiologia , Proliferação de Células , Sobrevivência Celular/fisiologia , Células Cultivadas , Corioide/citologia , Modelos Animais de Doenças , Hipóxia/metabolismo , Macaca mulatta , Reação em Cadeia da Polimerase em Tempo Real , Receptores CXCR4/fisiologia , Retina/citologia
10.
Eur J Surg Oncol ; 38(7): 602-10, 2012 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-22417704

RESUMO

BACKGROUND: The prognosis of patients with intrahepatic cholangiocarcinoma (ICC) is currently unsatisfactory. The aims of this study were to identify prognostic factors after curative ICC resection, and to evaluate the effects of postoperative transcatheter arterial chemoembolisation (TACE). METHODS: A retrospective analysis was conducted of 114 ICC patients who underwent curative resection from January 2005 to December 2006. Relationships between survival and clinicopathological factors were evaluated using univariate and multivariate analyses. The benefits of adjuvant TACE were investigated separately. RESULTS: The cumulative 1-, 3-, and 5-year survival rates were 63%, 26%, and 15%, respectively. Multivariate analysis revealed that tumour size ≥ 5 cm (hazard ratio [HR] 1.875, 95% CI 1.139-3.088, P=0.013) and advanced TNM stage (stage III or IV) (HR 1.681, 95% CI 1.035-2.732, P=0.036) were independently associated with poor prognosis. Fifty-seven patients underwent adjuvant TACE. In patients with poor prognostic factors, TACE improved the survival rate (P<0.001). However, in patients without poor prognostic factors, TACE did not significantly change the survival rate (P=0.724). CONCLUSIONS: Postoperative adjuvant TACE can prolong survival in ICC patients with tumour size ≥ 5 cm or advanced TNM stage.


Assuntos
Neoplasias dos Ductos Biliares/terapia , Ductos Biliares Intra-Hepáticos , Quimioembolização Terapêutica , Colangiocarcinoma/terapia , Artéria Hepática , Idoso , Análise de Variância , Neoplasias dos Ductos Biliares/etiologia , Neoplasias dos Ductos Biliares/patologia , Neoplasias dos Ductos Biliares/cirurgia , Biomarcadores Tumorais/análise , Antígeno CA-19-9/análise , Quimioembolização Terapêutica/métodos , Quimioterapia Adjuvante , Colangiocarcinoma/etiologia , Colangiocarcinoma/patologia , Colangiocarcinoma/cirurgia , Feminino , Hepatite Viral Humana/complicações , Humanos , Cirrose Hepática/complicações , Masculino , Pessoa de Meia-Idade , Invasividade Neoplásica , Estadiamento de Neoplasias , Prognóstico , Estudos Retrospectivos , Fatores de Risco , Análise de Sobrevida
11.
Transplant Proc ; 43(5): 1953-61, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21693306

RESUMO

OBJECTIVE: Partial liver transplantation is currently gaining wider acceptance, which alleviates donor organ shortage, but also reveals the problem of small-for-size (SFS) syndrome. The precise mechanism behind it remains unknown. Large animal models for SFS syndrome are being developed using veno-venous bypass (VVB), however, splenectomies have often become necessary making the models useless for clinical situations. This study establishes a clinically well-simulated and effective model of SFS graft injury without VVB. METHODS: In this study, 30% and 100% of liver grafts were orthotopically transplanted to pigs in groups A (n = 12) and B (n = 5), respectively, both without VVB. Intraoperative hemodynamics and metabolic parameters were assessed consecutively. The operative survival rates were evaluated during 7 days of follow-up as well as the serum biochemical profiles, the kinesis of portal pressure gradient, and the pathological findings. RESULTS: All the recipients survived the anhepatic period except one in group A who died of irretrievable acidosis. The tolerance rate for non-VVB were 91.7% (11/12) in group A and 100% (5/5) in group B with no significant differences. The 7-day survival rate in group A was significantly less than that for group B (50% versus 100%, P < .05) with more prolonged prothrombin times, increased bilirubin and alanine aminotransferase levels, and persistantly higher values of portal pressure gradient during almost the entire follow-up period. Accordingly, the pathological findings clarified more severe microvascular impairments in group A than group B. CONCLUSION: These data suggest that the model of pigs transplanting with 30% liver grafts without VVB is safe and reproducible. The good clinical simulation on operative procedures and clinicopathological performance indicates it is a more rational model for further research on SFS syndrome.


Assuntos
Transplante de Fígado , Modelos Biológicos , Procedimentos Cirúrgicos Vasculares , Animais , Feminino , Hemodinâmica , Testes de Função Renal , Testes de Função Hepática , Suínos , Porco Miniatura
12.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 28(5): 284-5, 319, 1993 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-8194417

RESUMO

The cellular DNA content of 28 cases of salivary adenoid cystic carcinoma (SACC) were quantified with the flow cytometric analysis. The result showed that majority of SACC (22/28) were diploidy tumors. No significant correlation between the cellular DNA content and the histopathologic appearances, the cellular DNA content and the clinical behavior of SACC was found.


Assuntos
Carcinoma Adenoide Cístico/patologia , Neoplasias das Glândulas Salivares/patologia , Adolescente , Adulto , Idoso , Aneuploidia , Carcinoma Adenoide Cístico/genética , DNA de Neoplasias/genética , Diploide , Feminino , Citometria de Fluxo , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias das Glândulas Salivares/genética
13.
Yao Xue Xue Bao ; 24(9): 690-6, 1989.
Artigo em Chinês | MEDLINE | ID: mdl-2618719

RESUMO

Determination method of allopurinol has been studied by the second derivative oscillopolarography. Well-defined linear relationship between concentrations and the wave height in the range 3 X 10(-7)-1 X 10(-4) mol/L and 5 X 10(-7)-1 X 10(-4) mol/L was observed, respectively, in 0.50 mol/L H2SO4 and pH 5.5 HAc-NaAc buffer. The contents of allopurinol in tablets and urine were determined. The character of the electrode reaction was explored preliminarily.


Assuntos
Alopurinol , Alopurinol/análise , Alopurinol/urina , Polarografia/métodos , Comprimidos
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA