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1.
Mol Med Rep ; 22(1): 117-126, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32377732

RESUMO

Acute liver failure (ALF) is a fatal liver disease characterized by severe hepatocyte destruction. MicroRNAs (miRNAs/miRs) have been reported to serve a key role in a number of liver diseases. Therefore, the aim of the present study was to investigate the role and underlying mechanism of miR­214 in ALF. ALF murine and hepatocyte models were established using D­galactosamine (D­GalN) and lipopolysaccharide (LPS) or D­GalN + tumor necrosis factor (TNF)­α, respectively. The expression levels of miR­214 and Bax were detected by reverse transcription­quantitative polymerase chain reaction (RT­qPCR) and/or western blotting. Furthermore, an automatic biochemical analyzer was used to measure the levels of aspartate aminotransferase (AST) or alanine aminotransferase (ALT). The levels of TNF­α and interleukin (IL)­6 were detected by ELISA and RT­qPCR. In addition, TUNEL staining and flow cytometry were used to analyze cell apoptosis, and the protein expression of caspase­3 was determined by western blotting. It was identified that the levels of AST and ALT were increased and that hepatocyte apoptosis was enhanced in the D­GalN/LPS­stimulated group compared with the control. Furthermore, higher expression of caspase­3 was observed in the D­GalN/LPS­stimulated group. In addition, it was demonstrated that miR­214 was downregulated, while Bax was upregulated in D­GalN/LPS­stimulated mice and D­GalN/TNF­α­stimulated BNLCL2 cells. Moreover, in D­GalN/TNF­α­stimulated BNLCL2 cells, miR­214 overexpression suppressed apoptosis and decreased TNF­α and IL­6 levels, and these effects were reversed by the Bax plasmid. It was also identified that overexpression of miR­214 significantly decreased Bax mRNA and protein expression levels in vitro. Collectively, the present results suggested that miR­214 inhibited hepatocyte apoptosis during ALF development via targeting Bax, thus indicating that miR­214 may be a potential target for ALF treatment.

2.
Sci Total Environ ; 729: 138876, 2020 Apr 23.
Artigo em Inglês | MEDLINE | ID: mdl-32361445

RESUMO

The oil and bacteria adhesion during membrane separation process brings great challenges to the operation costs and membrane service life. Meantime, the strong chemical corrosion in sewage seriously limits the durability of membrane as well. Herein, a facile strategy is developed for fabricating highly stable and efficient zwitterionic nanofibrous membrane (NFM) with self-cleaning feature via the combination of in-situ cross-linking of poly (sulfobetaine methacrylate) (PSBMA) and electrospun poly (ether sulfone) (PES) nanofibers. Owing to the introduction of zwitterionic functional groups, the PSBMA/PES NFM exhibits superior antifouling ability (over 3 cycles of crude oil fouling/self-cleaning and up to 7 days of bacteria adhesion/repelling tests). Moreover, the membrane also presents remarkable chemical stability in acidic, alkaline and salty environments; and exhibits excellent separation performance for both layered oil/water mixture and oil-in-water emulsion as well. Furthermore, the membrane is capable to remove bacteria during the continuous oil/water mixture separation. Overall, the proposed strategy provides a new perspective into developing long-term antifouling membrane materials for complicated oily wastewater remediation in various corrosive environments.

3.
J Colloid Interface Sci ; 576: 1-9, 2020 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-32408158

RESUMO

Pathogen capture and removal from whole blood is a new strategy for extracorporeal blood purification, especially in initial treatment of sepsis before pathogen identification. Herein, hemocompatible magnetic particles with broad-spectrum bacteria capture capability were proposed for pathogen removal from whole blood, omitting the necessity of pathogen identification. Firstly, we designed and synthesized a new kind of imidazolium-based ionic liquid with good antibacterial activity, and polydopamine coating was utilized as a hemocompatible platform to immobilize ionic liquids on Fe3O4 nanoparticles, forming the hemocompatible magnetic particles (Fe3O4@PDA-IL). The magnetic particles exhibited good hemocompatibility and performed well in the removal of various species of clinically significant pathogens from human whole blood, including S. aureus, E. coli, and the hard-to-treat bacteria of P. aeruginosa and Methicillin-resistant S. aureus, which are the most common pathogens in bloodstream infections. Besides, the Fe3O4@PDA-IL particles were also capable to remove bacterial endotoxins from blood, inhibiting further aggravation of sepsis. Overall, we demonstrated the application of hemocompatible magnetic particles in the removal of pathogens and bacterial endotoxins from whole blood via electrostatic and hydrophobic interactions, without significant effects on blood cells or the activation of coagulation and complement, addressing the feasibility of using imidazolium-based ionic liquids for bacteria capture and removal from whole blood. It would contribute to the development of magnetic separation-based approaches to remove bacteria and bacterial endotoxin for extracorporeal blood purification, especially in initial sepsis therapy before pathogen identification.

4.
Chem Rec ; 2020 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-32286725

RESUMO

Manipulation and engineering of the surfaces has a key role in improving the materials properties. Anchoring of thin hydrogels on the materials surface is one of the recently developed methods to achieve surfaces with high potential applications. Layer-by-layer (LBL) has been used widely as a strong strategy for immobilization of thin hydrogel films on the surface of various organic/inorganic substrates. Electrostatic LBL and covalent LBL are two main strategies used in this regard. In electrostatic LBL, negatively and positively hydrophilic polymers are sequentially assembled to create a multilayer hydrogel which subsequent covalent crosslinking of multilayers improved the stability of the inserted layers. On the other hand, covalent LBL requires hydrophilic polymers bearing reactive telechelic groups. These reactive polymers are prepared by various polymerization techniques or by post-functionalization of biopolymers. The principles of hydrogel anchoring have described along with representative examples. Besides, the potential applications of the modified surfaces in specific cases have been addressed and overviewed.

5.
Mater Sci Eng C Mater Biol Appl ; 109: 110516, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32228930

RESUMO

Here, we report the application of mussel-inspired surface coating to prepare heparin-mimetic biomacromolecules modified magnetic Fe3O4 nanoparticles as recyclable anticoagulant. Sodium alginate sulfate (SAS), which has the similar chemical structure and bioactivity with heparin, was synthesized at first. Dopamine (DA) was then grafted onto the backbone of SAS as the mussel-inspired adhesive macromolecule (DA-g-SAS), followed by being coated onto Fe3O4 nanoparticles. The SAS coated Fe3O4 nanoparticles could combine both the advantages of magnetic responsiveness and blood compatibility. The measurements revealed that the modified nanoparticles showed improved anticoagulant property as well as good recyclable property. The study provides a promising method to introduce nanomaterials into the field of hemodialysis. And the heparin-mimic polysaccharide biomacromolecules modified Fe3O4 nanoparticles can be considered as attractive material for potential application for hemodialysis.

6.
Dig Dis Sci ; 2020 Mar 06.
Artigo em Inglês | MEDLINE | ID: mdl-32140946

RESUMO

BACKGROUND: Hepatitis B virus-related acute-on-chronic liver failure (HBV-ACLF) is a dynamic but reversible disease. AIM: We aimed to clarify whether the change in Chinese Group on the Study of Severe Hepatitis B-ACLF (COSSH-ACLF) grade in HBV-ACLF patients can be used to predict prognosis, and to explore the appropriate conditions for performing urgent liver transplantation. METHODS: We assessed the COSSH-ACLF grades of HBV-ACLF patients at different time points from June 2013 to May 2019 at Huashan Hospital in Shanghai, China, and analyzed the relationship between the change in grade and patient prognosis. RESULTS: A total of 207 HBV-ACLF patients were enrolled, of which 79 underwent urgent liver transplantation. Their COSSH-ACLF grades were calculated at diagnosis, 3-7 days after diagnosis, and on the final day. Most of the final ACLF grades were consistent with their corresponding grades at days 3-7 after diagnosis (62.5%), while only 44.5% were in accordance with the initial grades at diagnosis. In patients who had a poor prognosis (initial ACLF-3 and ACLF-2 or -3 at days 3-7), the 28-day survival rate was 93.3% in those who underwent transplantation and 6.8% in those who did not (P < 0.0001). However, in patients who had a good prognosis (ACLF-0 or ACLF-1 at days 3-7), the 28-day survival rate was 100% in transplanted patients and 91.5% in non-transplanted patients (P = 0.236). CONCLUSIONS: Reevaluation of the COSSH-ACLF grade 3-7 days after diagnosis could potentially show an indication for urgent liver transplantation.

7.
ACS Appl Mater Interfaces ; 12(12): 13698-13708, 2020 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-32129070

RESUMO

Recent emerged metal-organic frameworks (MOFs), as superior drug carriers, provide novel strategies to combat pathogenic bacterial infections. Although various antibacterial metal ions can be easily introduced in MOFs for chemical bacterial ablation, such a single-model bactericidal method suffers from high-dose use, limited antibacterial efficiency, and slow sterilization rate. Hence, developing a dual bactericidal system is urgently required. Herein, we report an MOF/Ag-derived nanocomposite with efficient metal-ion-releasing capability and robust photo-to-thermal conversion effect for synergistic sterilization. The MOF-derived nanocarbon consisting of metallic zinc and a graphitic-like carbon framework is first synthesized, and then Ag nanoparticles (AgNPs) are evenly introduced via the displacement reaction between Zn and Ag+. Upon near-infrared irradiation, the fabricated nanoagents can generate massive heat to destroy bacterial membranes. Meanwhile, abundant Zn2+ and Ag+ ions are released to make chemical damage to bacterial intracellular substances. Systematic antibacterial experiments reveal that such dual-antibacterial effort can endow the nanoagents with nearly 100% bactericidal ratio for highly concentrated bacteria at a very low dosage (0.16 mg/mL). Furthermore, the nanoagents exhibit less cytotoxicity, which provides potential possibilities for the applications in the biological field. In vivo assessment indicates that the nanocomposites can realize rapid and safe wound sterilization and are expected to be an alternative to antibiotics. Overall, we present an easily fabricated structure-engineered nanocomposite with chemical and photothermal effects for broad-spectrum bacterial sterilization.

8.
J Mater Chem B ; 8(9): 1960-1970, 2020 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-32067017

RESUMO

Recently emerged hemoperfusion absorbents, e.g. ion-exchange resin, activated carbon, and other porous materials, provide numerous novel possibilities to cure chronic liver failure (CLF) and renal failure (CRF). However, the limited adsorption performance and unsatisfactory blood compatibility significantly impede the development of the absorbents. Hence, designing safe and self-anticoagulant hemoperfusion absorbents with robust toxin clearance remains a considerable challenge. Here, brand new Kevlar-based composite gel beads for hemoperfusion are prepared by interface assembly based on π-π interaction. First, Kevlar nanofiber-graphene oxide (K-GO) beads are produced by liquid-liquid phase separation. Then, sodium p-styrenesulfonate (SS) is adsorbed onto the K-GO interface by π-π interaction and initiated to achieve the composite gel (K-GO/PSS) beads with an interfacial crosslinked structure. Such composite gel beads possess superior mechanical strength and self-anticoagulation capability, owing to the dual-network structure and heparin-mimicking gel structure, respectively. Furthermore, the K-GO/PSS beads show robust adsorption capacities for different kinds of toxins due to their strong charge and π-π interactions. A simulated hemoperfusion experiment in vitro demonstrates that the concentrations of the toxins in the blood can be restored to normal values within 30 minutes. In general, we envision that such composite gel beads will provide new strategies for future clinical CLF and CRF treatments.

9.
Biomacromolecules ; 21(5): 1762-1775, 2020 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-31955566

RESUMO

Current therapy for liver failure and concomitant hyperbilirubinemia faces the challenge of poor hemocompatibility and bleeding risks associated with the anticoagulant injection. Herein, heparin-mimetic biomacromolecule (HepMBm) with a similar degree of sulfation and anticoagulant properties to heparin was synthesized by imitating the structure of natural biomacromolecule heparin. Then HepMBm was used to prepare nanocomposite spheres based on reduced graphene oxide (rGO). The formation of a dual-network structure in the spheres endowed the spheres with improved dimensional stability. The proposed spheres exhibited outstanding blood compatibilities and excellent self-anticoagulant properties. The bilirubin adsorption experiments and whole blood bilirubin removal assay indicated that the spheres exhibited high bilirubin removal capability from whole blood (The removal ratio was 99.69%.). The spheres open new routes for a therapeutic strategy without a plasma separation system and heparin pump, which may be a step toward a lightweight wearable artificial liver.

10.
J Mater Chem B ; 8(3): 568-577, 2020 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-31854426

RESUMO

Rapid endothelialization and prevention of restenosis are two vital challenges for the preparation of a small-diameter vascular graft (SDVG), while postoperative infection after implantation is often neglected. In the present study, carboxymethyl chitosan (CMC) and chitosan (CS) were chosen as the anti-thrombotic and anti-bacterial components, respectively; and then an asymmetric vascular graft was fabricated by co-electrospinning of poly(ε-caprolactone) (PCL)/CMC and PCL/CS. The mechanical properties of the asymmetric vascular graft were much better than those of the native vessels. In vitro blood compatibility tests indicated that the inner layer of the graft could inhibit thrombosis effectively. The outer layer of the graft had a certain anti-bacterial effect owing to the addition of chitosan. Besides, the inner layer of the graft could greatly promote the growth of endothelial cells. It is believed that the asymmetric SDVG with anti-thrombotic and anti-bacterial functions could contribute to the future clinical implantation of tissue engineered vascular grafts.

11.
Colloids Surf B Biointerfaces ; 186: 110728, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31862559

RESUMO

In this study, we proposed a green, facile and low-cost approach for the fabrication of multifunctional particles with robust bacteria removal capability and on-demand biofilm eradication activity. Based on mussel-inspired coating of catechol and hexanediamine on Fe3O4 in aqueous solution, magnetic poly(catechol-hexanediamine) particles (Fe3O4@HDA) were prepared successfully in 1 h, at room temperature. Microbiological experiments demonstrated the Fe3O4@HDA particles could capture bacteria in water efficiently. Meanwhile, with an integration of magnetic response property and near-infrared-triggered photothermal bactericidal activity, the Fe3O4@HDA particles showed a high potential for biofilm targeting and in-situ eradication. We believe that the rationally designed magnetic poly(catechol-hexanediamine) particles could extend the applications of smart antimicrobial agents to industrial fields such as water disinfection and biofouling clean-up.

12.
J Autoimmun ; : 102372, 2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31810856

RESUMO

The genetic association of primary biliary cholangitis with major histocompatibility complex (MHC) has been widely confirmed among different ethnicities. To map specific MHC region variants associated with PBC in a Han Chinese cohort, we imputed HLA antigens and amino acids (AA) in 1126 PBC cases and 1770 healthy control subjects using a Han-MHC reference database. We demonstrate that HLA-DRB1 and/or HLA-DQB1 contributed the strongest signals, and that HLA-DPB1 was a separate independent locus. Regression analyses with classical HLA alleles indicate that HLA-DQB1*03:01 or HLA-DQß1-Pro55, HLA-DPB1*17:01 or HLA-DPß1-Asp84 and HLA-DRB1*08:03 could largely explain MHC association with PBC. Forward stepwise regression analyses with HLA amino acid variants localize the major signals to HLA-DRß1-Ala74, HLA-DQß1-Pro55 and HLA-DPß1-Asp84. Electrostatic potential calculations implicated AA variations at HLA-DQß1 position 55 and HLA-DPß1 position 84 as critical to peptide binding properties. Furthermore, although several critical Han Chinese AA variants differed from those shown in European populations, the predicted effects on antigen binding are likely to be very similar or identical and underlie the major component of MHC association with PBC.

13.
Huan Jing Ke Xue ; 40(11): 4755-4763, 2019 Nov 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854540

RESUMO

The mass concentration and chemical composition of fine particles were continuously observed on-line from October 31 to December 3, 2018 at Hebei Key Laboratory of Haze Pollution Prevention and Control in Shijiazhuang. The characteristics of haze pollution in autumn and winter in Shijiazhuang were analyzed. The results showed that during the observation period, four haze pollution episodes occurred with PM2.5 as the primary pollutant, and the maximum daily concentration was 154, 228, 379, and 223 µg·m-3, respectively, reaching a heavy pollution level or above. The main components of PM2.5were water-soluble inorganic ions (WSⅡ) and carbon-containing aerosols, accounting for (60.7±15.6)% and (21.6±9.7)% of PM2.5 mass concentration, respectively. Compared with clean days, the mass concentration of WSⅡ and carbon aerosol during haze pollution increased by 4.4 times and 3.1 times, respectively, which was the main cause of haze pollution. NO3-, SO42-, and NH4+(SNA) were the main components of WSⅡ, accounting for (91.5±17.3)% of the total WSⅡ concentration, of which NO3- took up the highest proportion. The explosive growth of SNA during haze pollution was the main reason for the extremely high PM2.5concentration. Under non-high humidity conditions, the formation rates of unit mass substrates (NO3-, SO42-) were not significantly different, but the transformation of SO42- was significantly promoted after the liquid phase oxidation of SO2 was triggered under high humidity conditions. The atmosphere in Shijiazhuang is rich in NH3, and the molar ratio of n(NH4+) to n(NO3-+2×SO42-) in PM2.5 was greater than 1. The presence of a large amount of NH3 could promote the transformation of NO3- and SO42- and aggravate pollution. During the haze pollution period, the accumulation of primary pollutants from coal and motor vehicles was the main reason for the increase in carbon-containing aerosol. Compared with clean days, the formation of SOC was inhibited. Before the beginning of the warm season, the mobile form was the main pollution source of PM2.5, contributing 30.8% and 39.8% of PM2.5 mass concentration. With the increase of coal combustion emissions, the contribution of coal-fired sources gradually increased to 25.5%, becoming the primary pollution source.

14.
Int J Biol Macromol ; 2019 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-31783078

RESUMO

Adsorbents are usually used to remove uremic toxins for blood purification. However, the removal of urea is still an intractable problem, since no effective material has been found for urea removal by physical adsorption. Here, urease immobilized graphene oxide core@shell heparin-mimicking polymer (U-GO-HMP) beads were designed, which exhibited good urea removal ability with a removal amount of about 635 mg/g and a removal ratio of about 80% from urea solution. In addition, urea could be removed from collected dialysate and the removal ratio could reach 60% within 480 min. Beyond that, the U-GO-HMP beads also showed good reusability with sustainable relative activity after 5 cycles. Furthermore, the U-GO-HMP beads exhibited good blood compatibility with low hemolysis ratio, suppressed complement activation and contact activation, as well as increased clotting times. It is worthy believing that the U-GO-HMP beads may have great potential in the field of blood purification for urea removal.

15.
Epigenomics ; 11(16): 1779-1794, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31755303

RESUMO

Aim: PIWI-interacting RNAs (piRNAs) play crucial roles in germline development and carcinogenesis. The expression patterns of piRNAs in pulmonary tuberculosis (PTB) are still unclear. Materials & methods: Small RNA sequencing was applied to investigate peripheral blood piRNA expression patterns in PTB patients and healthy individuals. Results: A total of 428 upregulated and 349 downregulated piRNAs were identified from PTB patients. Target genes of dysregulated piRNAs were mainly involved in transcription and protein binding. Dysregulated piRNAs were enriched in many pathways related with immunity. Many target genes were regulated by the same piRNAs. Nucleotide bias of these piRNAs showed that piRNAs in peripheral blood may be formed from the primary biogenesis pathway. Conclusion: Findings demonstrated that the PIWI-piRNA pathway is active in human peripheral blood, where it may represent a new player in the PTB pathogenesis.

16.
Chin Med J (Engl) ; 132(22): 2647-2656, 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31725459

RESUMO

BACKGROUND: Few data are available regarding the progression of liver disease and therapeutic efficacy in chronic hepatitis B virus (HBV) carriers infected by mother-to-child transmission (MTCT). This study aimed to investigate these two aspects by comparing the adult chronic HBV carriers in MTCT group with those in horizontal transmission group. METHODS: The 683 adult chronic HBV patients qualified for liver biopsy including 191 with MTCT and 492 with horizontal transmission entered the multi-center prospective study from October 2013 to May 2016. Biopsy results from 217 patients at baseline and 78 weeks post antiviral therapy were collected. RESULTS: Patients infected by MTCT were more likely to have e antigen positive (68.6% vs. 58.2%, χ = -2.491, P = 0.012) than those with horizontal transmission. However, in patients with MTCT, levels of alkaline phosphatase (ALP) (P = 0.031), Fibroscan (P = 0.013), N-terminal propeptide of Type III procollagen (PIIINP) (P = 0.014), and Laminin (LN) (P = 0.006) were high, in contrast to the patients with horizontal transmission for whom the levels of albumin (ALB) (P = 0.041), matrix metalloproteinase-3 (MMP-3) (P = 0.001) were high. The 47.2% of patients with MTCT and 36.8% of those with horizontal transmission had significant liver fibrosis (P = 0.013). Following antiviral therapy for 78 weeks, 21.2% and 38.0% patients with MTCT and horizontal transmission acquired hepatitis B e antigen (HBeAg) clearance, respectively (P = 0.043), and the virological response rates were 54.7% and 74.1% in the MTCT and horizontal groups, respectively (P = 0.005). MTCT was a risk factor for HBeAg clearance and virological response. CONCLUSION: Adult patients with MTCT were more prone to severe liver diseases, and the therapeutic efficacy was relatively poor, which underlined the importance of earlier, long-term treatment and interrupting perinatal transmission. TRIAL REGISTRATION: NCT01962155; https://clinicaltrials.gov.

17.
Colloids Surf B Biointerfaces ; 184: 110545, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31629184

RESUMO

pH-responsive nanocarriers such as polymeric micelles that self-assemble from amphiphilic copolymers containing amino groups have been limited by their significant effects on the blood and thus compromise of their hemocompatibility due to the amino group-induced positive charges. Here we report a biomimetic phosphorylcholine strategy to improve the hemocompatibility of the pH-responsive micelles with positive charges. Amphiphilic copolymers containing different number of tertiary amino groups were synthesized in five steps through ring opening polymerization, azide reaction, thio-bromo "Click" chemistry, and atom transfer radical polymerization to self-assemble biomimetic phosphorylcholine micelles with pH-responsiveness, which shown no significant effects on red blood cells, coagulation, and platelet activation. Moreover, albumin adsorption on the micelles was significantly lower than that of polycaprolactone-methoxypolyethylene glycol (PCL-mPEG) control, and in terms of immune cells, the micelles showed controllable phagocytosis that dependent on the number of tertiary amino groups, in which the one containing four tertiary amino groups in its corresponding copolymer remains had a lower phagocytosis by whole blood leukocyte than that of PCL-mPEG. Based on these results, the hemocompatibility related mechanism of the micelles was discussed and proposed. Our findings demonstrated that this biomimetic phosphorylcholine is a promising strategy to improve the hemocompatibility of the positively charged nanocarriers.

18.
ACS Appl Mater Interfaces ; 11(39): 36141-36153, 2019 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-31503444

RESUMO

Wound treatment is a long-lasting clinical issue. Poor angiogenesis leading to delayed wound closure causes huge challenges for healing. Functional electrospun membranes have been established as an efficient strategy to promote wound recovery by protecting and improving vascular regeneration. Here, we aimed to investigate the effect of tazarotene, an active drug for angiogenesis, loaded in aligned electrospun nanofibrous barrier on a soft tissue wound. This aligned membrane was arranged in a single direction, and tazarotene could be released from its nanofibers sustainably. The in vitro study demonstrated that compared with the random drug-loaded or other control groups, the aligned tazarotene-loaded membranes [poly-caprolactone (PCL)/AT] could stimulate proliferation, migration, angiogenesis, and vascular endothelial growth factor secretion and its gene expression of human umbilical vein endothelial cells. Furthermore, the in vivo model showed that the prepared tazarotene-loaded aligned membrane significantly accelerated the speed of healing, improved the neovascularization and re-epithelialization, and inhibited the inflammatory reaction in the wound area. All these results above indicated that the PCL/AT nanofibrous dressing, which could promote angiogenesis because of both stimulation of structure and chemical signals, is a promising wound-caring material.


Assuntos
Sistemas de Liberação de Medicamentos , Membranas Artificiais , Neovascularização Fisiológica/efeitos dos fármacos , Ácidos Nicotínicos , Pele , Cicatrização/efeitos dos fármacos , Animais , Células Endoteliais da Veia Umbilical Humana/metabolismo , Células Endoteliais da Veia Umbilical Humana/patologia , Humanos , Masculino , Ácidos Nicotínicos/química , Ácidos Nicotínicos/farmacologia , Ratos , Ratos Sprague-Dawley , Pele/lesões , Pele/metabolismo , Pele/patologia
19.
J Colloid Interface Sci ; 556: 492-502, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31473539

RESUMO

Given the complexity of pollutants in wastewater, development of facile and effective multifunctional materials, which can not only kill bacteria but also remove dyes from wastewater, is in high demand. Herein, a facile strategy for the preparation of positively-charged nanofibrous membranes (NFMs) is reported via the combination of electrospinning and in-situ cross-linked polymerization of poly ([2-(methacryloyloxy)-ethyl] trimethyl ammonium chloride) (PMETAC) in poly (ether sulfone) (PES) solution. The quaternary ammonium salt polymer of PMETAC enabled the NFMs with positive charge to kill bacteria and remove anionic dyes. The antibacterial tests including agar plate counting and live/dead staining indicate that the NFMs show strong antibacterial ability with bacterial killing ratios of nearly 99% for both Escherichia coli and Staphylococcus aureus, as well as remarkable recyclability towards killing bacteria. The dyes adsorption experiments show that the NFMs exhibit high adsorption capacity for anionic dyes up to 208 mg g-1 for Congo Red (CR) and good reusability toward CR. Impressively, the membrane adsorption column test indicates that the CR dye removal ratio is up to 100% for the first time, and that is still as high as 96.5% for the third time with a fresh dye solution. Given the above advantages, such fascinating NFMs may provide new perspectives in the exploitation of multifunctional membrane materials for complex water remediation.


Assuntos
Antibacterianos/química , Corantes/química , Escherichia coli/crescimento & desenvolvimento , Membranas Artificiais , Nanofibras/química , Polímeros/química , Staphylococcus aureus/crescimento & desenvolvimento , Sulfonas/química , Águas Residuárias , Adsorção , Águas Residuárias/química , Águas Residuárias/microbiologia
20.
Colloids Surf B Biointerfaces ; 181: 918-926, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31382341

RESUMO

Postoperative adhesion may form as the result of a complicated fibrosis and inflammatory response, thus leads to a series of complications or increases the risk of surgery failure. Herein, we prepared poly (lactic-co-glycolic acid)-graft-polyvinylpyrrolidone/polyiodide (PLGA-g-PVP/I) electrospun fibrous membranes to prevent postoperative adhesion and infection formation. Firstly, hydrophilic PVP molecules were grafted on the surface of PLGA powders by gamma ray, and then iodine ions were coordinated with the grafted PVP. Subsequently, PLGA-g-PVP/I fibrous membranes were prepared by electrospinning. The PLGA-g-PVP/I membranes were analyzed via UV-vis, FTIR, Raman, and XPS. The formed polyiodide endowed the membranes with sustained antibacterial activity. The antimicrobial property of PLGA-g-PVP/I membranes was ascribed to the synergistic effect of intracellular ROS production and glutathione oxidation. Furthermore, the prevention efficacy of postoperative abdominal adhesion from the PLGA-g-PVP/I composite membranes was characterized in a rat model of sidewall defect-cecum abrasion. The results demonstrated that the PLGA-g-PVP/I fibrous membranes could prevent the postoperative abdominal adhesion effectively. Therefore, to endow the PLGA-g-PVP/I electrospun fibrous membranes with durable antibacterial property may be a promising strategy towards an anti-bacterial and anti-adhesion system for commercial and clinical uses.


Assuntos
Antibacterianos/farmacologia , Materiais Biocompatíveis/farmacologia , Escherichia coli/efeitos dos fármacos , Ácido Periódico/química , Copolímero de Ácido Poliláctico e Ácido Poliglicólico/farmacologia , Povidona/química , Animais , Antibacterianos/química , Materiais Biocompatíveis/química , Proliferação de Células/efeitos dos fármacos , Fibroblastos/efeitos dos fármacos , Camundongos , Testes de Sensibilidade Microbiana , Estresse Oxidativo/efeitos dos fármacos , Copolímero de Ácido Poliláctico e Ácido Poliglicólico/química , Ratos , Raios Ultravioleta
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