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1.
J Exp Bot ; 71(1): 305-317, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31559426

RESUMO

Suberin is a cell-wall biopolymer with aliphatic and aromatic domains that is synthesized in the wound tissues of plants in order to restrict water loss and pathogen infection. ω-hydroxyacid/fatty alcohol hydroxycinnamoyl transferase (FHT) is required for cross-linking of the aliphatic and aromatic domains. ABA is known to play a positive role in suberin biosynthesis but it is not known how it interacts with FHT. In this study, the kiwifruit (Actinidia chinensis) AchnFHT gene was isolated and was found to be localized in the cytosol. Transient overexpression of AchnFHT in leaves of Nicotiana benthamiana induced massive production of ferulate, ω-hydroxyacids, and primary alcohols, consistent with the in vitro ability of AchnFHT to catalyse acyl-transfer from feruloyl-CoA to ω-hydroxypalmitic acid and 1-tetradecanol. A regulatory function of four TFs (AchnABF2, AchnMYB4, AchnMYB41, and AchnMYB107) on AchnFHT was identified. These TFs localized in the nucleus and directly interacted with the AchnFHT promoter in yeast one-hybrid assays. Dual-luciferase analysis indicated that AchnABF2, AchnMYB41, and AchnMYB107 activated the AchnFHT promoter while AchnMYB4 repressed it. These findings were supported by the results of transient overexpression in N. benthamiana, in which AchnABF2, AchnMYB41, and AchnMYB107 induced expression of suberin biosynthesis genes (including FHT) and accumulation of suberin monomers, whilst AchnMYB4 had the opposite effect. Exogenous ABA induced the expression of AchnABF2, AchnMYB41, AchnMYB107, and AchnFHT and induced suberin monomer formation, but it inhibited AchnMYB4 expression. In addition, fluridone (an inhibitor of ABA biosynthesis) was found to counter the inductive effects of ABA. Activation of suberin monomer biosynthesis by AchnFHT was therefore controlled in a coordinated way by both repression of AchnMYB4 and promotion of AchnABF2, AchnMYB41, and AchnMYB107.

2.
J Proteomics ; 213: 103614, 2020 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-31846764

RESUMO

Lysine methylation is a widespread protein post-translational modification showing essentialities in versatile cellular process. EZH2, a methyltransferase specifically trimethylates the lysine 27 of histone H3 and its aberrance in several cancers promotes the development of its inhibitors against hematological tumors. In this study, we presented a deep exploration of lysine mono-, di- and trimethylomes in EZH2 wild-type and Y641 mutant lymphoma cell lines. Our results showed that several substrates were modified in different methylation levels. Moreover, these methylated lysine residues could also undergo other types of PTMs. Combined with the differences proved in protein expression, lysine acetylation, lysine ubiquitylation and protein N-termianl acetylation level, our study underlined the substrate specificity of lysine methylation and its crosstalk with other types of PTMs. Totally, our study raised new insights into the global cellular methylation features in hematological cell lines, which provided further inspects into the distribution and function of lysine methylation. SIGNIFICANCE: Our study showed the global landscape of mono-, di- and trimethylomes in the EZH2-aberrant DLBCL cell lines, revealing the molecular characteristics of lysine methylation. Combined with the protein abundance and potential crosstalk among different types of PTMs, our study raised new insights into the global cellular methylation features in hematological tumors and provided further inspects into the distribution and function of lysine methylation.

3.
Nat Commun ; 10(1): 4861, 2019 10 24.
Artigo em Inglês | MEDLINE | ID: mdl-31649241

RESUMO

Achieving the activation of drugs within cellular systems may provide targeted therapies. Here we construct a tumour-selective cascade activatable self-detained system (TCASS) and incorporate imaging probes and therapeutics. We show in different mouse models that the TCASS system accumulates in solid tumours. The molecules show enhanced accumulation in tumour regions via the effect of recognition induced self-assembly. Analysis of the molecular penetration in tumour tissue shows that in vivo self-assembly increases the penetration capability compared to typical soft or hard nanomaterials. Importantly, the in vivo self-assembled molecules exhibit a comparable clearance pathway to that of small molecules, which are excreted from organs of the reticuloendothelial system (liver and kidney), while are relatively slowly eliminated from tumour tissues. Finally, this system, combined with the NIR probe, shows high specificity and sensitivity for detecting bladder cancer in isolated intact patient bladders.

4.
Angew Chem Int Ed Engl ; 58(43): 15287-15294, 2019 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-31402550

RESUMO

Tumor complexity makes the development of highly sensitive tumor imaging probes an arduous task. Here, we construct a peptide-based near-infrared probe that is responsive to fibroblast activation protein-α (FAP-α), and specifically forms nanofibers on the surface of cancer-associated fibroblasts (CAFs) in situ. The assembly/aggregation-induced retention (AIR) effect results in enhanced accumulation and retention of the probe around the tumor, resulting in a 5.5-fold signal enhancement in the tumor 48 h after administration compared to that of a control molecule that does not aggregate. The probe provides a prolonged detectable window of 48 h for tumor diagnosis. The selective assembly of the probe results in a signal intensity over four- and fivefold higher in tumor than in the liver and kidney, respectively. With enhanced tumor imaging capability, this probe can visualize small tumors around 2 mm in diameter.

5.
Endocr J ; 66(7): 629-636, 2019 Jul 28.
Artigo em Inglês | MEDLINE | ID: mdl-31092739

RESUMO

Increasing of arterial stiffness is the pathophysiological characteristic of hypertension. Carotid-femoral pulse wave velocity (CF-PWV) is an index of arterial stiffness. Serum uric acid has been found to be involved the development of hypertension. We investigated the relationship between CF-PWV and serum uric acid in subjects with hypertension and hyperuricemia. 651 subjects (M/F 271/380) were divided into four groups, group 1: subjects without hypertension and hyperuricemia; group 2: hypertension subjects without hyperuricemia; group 3: hyperuricemia subjects without hypertension; group 4: subjects with hypertension and hyperuricemia. CF-PWV was measured by Complior apparatus. Results showed that levels of CF-PWV (10.75 ± 2.03 vs. 10.06 ± 1.98 m/s, p < 0.001) and serum uric acid (319.33 ± 80.12 vs. 298.78 ± 74.88 umol/L, p = 0.001) were significantly higher in hypertensive (groups 2 + 4) group than in normotensive (groups 1 + 3) group. CF-PWV was significantly higher in group 4 than group 1, group 2 and group 3 (ANOVA analysis: F = 13.348, p < 0.001; 11.78 ± 2.10 vs. 9.98 ± 1.98, 10.52 ± 1.93, 10.56 ± 1.99 m/s, all p < 0.05, respectively). There was positive correlation between CF-PWV and serum uric acid in entire study group (r = 0.187, p < 0.001), even after adjusting for gender, body mass index, systolic blood pressure (SBP) and diastolic blood pressure (r = 0.100, p = 0.015). Multiple linear regressions showed that SBP, age, benzbromarone, statin and serum uric acid were independent associating factors of CFPWV in all subjects (ß = 0.310, p < 0.001; ß = 0.330, p < 0.001; ß = 0.172, p = 0.002; ß = -0.143, p = 0.006; ß = 0.126, p = 0.027; respectively). In conclusions, CF-PWV was significantly higher in hypertension subjects with hyperuricemia compared to hypertension without hyperuricemia subjects, and serum uric acid was an independent associating factor of CF-PWV.


Assuntos
Hipertensão , Hiperuricemia , Ácido Úrico/sangue , Idoso , Estudos Transversais , Feminino , Humanos , Hipertensão/sangue , Hipertensão/complicações , Hipertensão/fisiopatologia , Hiperuricemia/sangue , Hiperuricemia/complicações , Hiperuricemia/fisiopatologia , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Rigidez Vascular/fisiologia
6.
J Neurochem ; 150(4): 441-452, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30851189

RESUMO

While glucagon-like peptide-1 (GLP-1) was reported to have a positive impact on Parkinson disease, it is extremely short half-life greatly hindered its clinical use. In this study, the mouse strain MG1363-pMG36e-GLP-1 was engineered to continuously express GLP-1 to treat Parkinson disease in a 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP)-treated Parkinson disease model. In our study, oral supplementation with MG1363-pMG36e-GLP-1 significantly (p < 0.05) reduced MPTP-induced locomotor impairments, increased tyrosine hydroxylase-positive neurons, suppressed microglia and astrocyte activation, and down-regulated expression of several inflammation-related molecules. In addition, MG1363-pMG36e-GLP-1 significantly (p < 0.01) reduced intestinal pathogen Enterobacteriaceae and markedly enhanced the number of probiotic Lactobacillus and Akkermansia. These data suggest that MG1363-pMG36e-GLP-1 could be a novel therapeutic means for Parkinson disease.

7.
Biochem J ; 476(3): 467-481, 2019 02 05.
Artigo em Inglês | MEDLINE | ID: mdl-30617221

RESUMO

MOV10 has emerged as an important host antiviral factor. MOV10 not only inhibits various viruses, including human immunodeficiency virus type 1, hepatitis C virus and vesicular stomatitis virus, but also restricts the activity of retroelements long interspersed nucleotide element-1, Alu, SVA and intracisternal A particles. Here, we report that MOV10 suppresses influenza A virus infection through interacting with viral nucleoprotein (NP), sequestering viral RNP in the cytoplasm and causing the degradation of viral vRNA. The antiviral activity of MOV10 depends on the integrity of P-bodies. We also found that the antiviral activity of MOV10 is partially countered by viral NS1 protein that interferes with the interaction of MOV10 with viral NP and causes MOV10 degradation through the lysosomal pathway. Moreover, NS1-defective influenza A virus is more susceptible to MOV10 restriction. Our data not only expand the antiviral spectrum of MOV10 but also reveal the NS1 protein as the first viral antagonist of MOV10.


Assuntos
Citoplasma/metabolismo , Vírus da Influenza A/metabolismo , Proteólise , RNA Helicases/metabolismo , Ribonucleoproteínas/metabolismo , Proteínas não Estruturais Virais/metabolismo , Células A549 , Citoplasma/genética , Células HEK293 , Humanos , Vírus da Influenza A/genética , Lisossomos/genética , Lisossomos/metabolismo , RNA Helicases/genética , Ribonucleoproteínas/genética , Proteínas não Estruturais Virais/genética
8.
Appl Microbiol Biotechnol ; 103(2): 929-939, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30448904

RESUMO

The strong human immunity and the associated toxicities of attenuated Salmonella severely limit the clinical use of Salmonella in tumour suppression. In the present study, we constructed an engineered VNP20009-DNase I strain and evaluated the synergistic effects of triptolide (TPL) and VNP20009-DNase I against melanoma in mice. Our results indicated that TPL could significantly inhibit the cell growth and cell migration and significantly enhanced the apoptosis rate of B16F10 cells in vitro. The in vivo results indicated that TPL markedly improved tumour colonisation of VNP20009-DNase I and led to a larger necrotic area in the melanoma. Moreover, the combination therapy significantly suppressed tumour volume and prolonged the life span of mice (P < 0.05) by upregulating the expression of Bcl-2/Bax and Caspase-3 and by downregulating the TLR4/NF-κB signalling, the expression of p-AKT/AKT and the production of proinflammatory factors. Therefore, the sound synergistic anti-tumour effects of TPL and VNP20009-DNase I indicate that the unconventional application of TPL and biological agents, approved by the China Food and Drug Administration (CFDA), can result in improved anti-cancer therapeutic outcomes.


Assuntos
Antineoplásicos Alquilantes/administração & dosagem , Terapia Combinada/métodos , Desoxirribonuclease I/farmacologia , Diterpenos/farmacologia , Melanoma/terapia , Fenantrenos/farmacologia , Salmonella/enzimologia , Vacinas de DNA/administração & dosagem , Animais , Antineoplásicos Alquilantes/farmacologia , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Desoxirribonuclease I/genética , Modelos Animais de Doenças , Portadores de Fármacos/administração & dosagem , Compostos de Epóxi/farmacologia , Vetores Genéticos , Humanos , Camundongos , Modelos Biológicos , Plasmídeos/administração & dosagem , Salmonella/genética , Infecções por Salmonella/microbiologia , Resultado do Tratamento , Vacinas de DNA/farmacologia
9.
Hum Pathol ; 86: 155-162, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30594747

RESUMO

The immunosuppressive effect of the programmed death (PD)-1/PD-L1 pathway plays an important role in the treatment of a variety of tumors, such as lung and breast cancer, but there is little literature about PD-1/PD-L1 in pheochromocytomas/paragangliomas (PCCs/PGLs). We explored the relationship of PD-L1 and malignant behavior in 77 cases of PCC/PGL using immunohistochemistry (IHC) to assess protein expression and RNAscope to detect mRNA expression in 20 cases. The IHC data showed that 59.74% of the PCCs/PGLs expressed PD-L1, and the extent of expression was highly correlated with Ki-67 (P = .019) and hypertension (P = .013) but not with age, sex, tumor size, capsular invasion, tumor necrosis, relapse/distant metastasis, secretion of noradrenaline/adrenaline/dopamine, or diabetes mellitus. In addition, we found an excellent correlation of PD-L1 mRNA and protein expression with a κ coefficient of 0.828, and further stratification of the IHC and RNAscope findings showed high consistency (Pearson coefficient 0.753). The correlation of PD-L1 and Ki-67 indicated that PD-L1 could be considered a malignant proliferation biomarker for PCCs/PGLs, which would be a putative biomarker for anti-PD-L1 therapies.


Assuntos
Neoplasias das Glândulas Suprarrenais/metabolismo , Antígeno B7-H1/metabolismo , Paraganglioma/metabolismo , Feocromocitoma/metabolismo , Neoplasias das Glândulas Suprarrenais/patologia , Adulto , Biomarcadores Tumorais/metabolismo , Proliferação de Células , Feminino , Humanos , Antígeno Ki-67/metabolismo , Masculino , Paraganglioma/patologia , Feocromocitoma/patologia
10.
Int J Biol Macromol ; 126: 209-214, 2019 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-30590141

RESUMO

In current study, a polysaccharide (GPP) was successfully extracted from Gynostemma pentaphyllum herb. Monosaccharide composition of GPP was rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid in a molar ratio of 4.11: 7.34: 13.31: 20.99: 1.07: 0.91: 4.75: 0.36. Molecular weight and polydispersity (Mw/Mn) of GPP were 4.070 × 104 Da and 1.037, respectively. Primary structure features of GPP were determined to be a polysaccharide by FT-IR and NMR. Fasting blood sugar of diabetic mice decreased from 17.56 mmol/L to 7.42 mmol/L by orally administration of 0.5 mL GPP (1 mg/mL) for 30 days. GPP exhibited a dose-dependent inhibition effect on α-glucosidase activity. Moreover, GPP could inhibit the glucose absorption and affect the protein expression of GLUT2, but not the protein expression of SGLT1. These results indicated GPP could be used as an effective ingredient to prevent and cure diabetes.


Assuntos
Gynostemma/química , Hipoglicemiantes/farmacologia , Polissacarídeos/isolamento & purificação , Polissacarídeos/farmacologia , Absorção Fisiológica , Animais , Células CACO-2 , Diabetes Mellitus Experimental/tratamento farmacológico , Glucose/metabolismo , Transportador de Glucose Tipo 2/metabolismo , Inibidores de Glicosídeo Hidrolases/farmacologia , Humanos , Hipoglicemiantes/uso terapêutico , Espectroscopia de Ressonância Magnética , Camundongos , Modelos Biológicos , Peso Molecular , Monossacarídeos/análise , Polissacarídeos/uso terapêutico , Transportador 1 de Glucose-Sódio/metabolismo , Espectroscopia de Infravermelho com Transformada de Fourier , alfa-Glucosidases/metabolismo
11.
Comput Intell Neurosci ; 2018: 3803627, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30210533

RESUMO

Face recognition (FR) with single sample per person (SSPP) is a challenge in computer vision. Since there is only one sample to be trained, it makes facial variation such as pose, illumination, and disguise difficult to be predicted. To overcome this problem, this paper proposes a scheme combined traditional and deep learning (TDL) method to process the task. First, it proposes an expanding sample method based on traditional approach. Compared with other expanding sample methods, the method can be used easily and conveniently. Besides, it can generate samples such as disguise, expression, and mixed variation. Second, it uses transfer learning and introduces a well-trained deep convolutional neural network (DCNN) model and then selects some expanding samples to fine-tune the DCNN model. Third, the fine-tuned model is used to implement experiment. Experimental results on AR face database, Extend Yale B face database, FERET face database, and LFW database demonstrate that TDL achieves the state-of-the-art performance in SSPP FR.


Assuntos
Face , Processamento de Imagem Assistida por Computador/métodos , Reconhecimento Automatizado de Padrão/métodos , Humanos , Aprendizado de Máquina
12.
Oncol Lett ; 16(4): 4297-4302, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30214564

RESUMO

This study aimed to investigate the correlation between CT features and liver function and p53 expression in hepatitis, cirrhosis and hepatocellular carcinoma (HCC). Forty patients with HCC, 30 patients with cirrhosis and 30 patients with chronic hepatitis were enrolled between December, 2015 and December, 2016. At the same time, normal liver tissues collected from 30 patients with hepatic hemangioma were used as the normal control group. All the patients were scanned by CT. Average body surface area, left outer lobe and caudate lobe volume, and the proportions of left outer lobe and caudate lobe to the whole liver were calculated. Biochemical indexes of liver function were determined. The pathological tissues of all the subjects were analyzed. Compared with the control group, total liver volume of the HCC group was significantly reduced (P<0.05). Compared with the HCC group, the volume of the left outer lobe increased in the hepatitis group and the cirrhosis group. Compared with control group, caudate lobe volume increased significantly in the hepatitis group (P<0.05). Compared with the control group, the volume of the left outer lobe and the proportion of caudate lobe to the whole liver volume was significantly increased in all three groups (P<0.05). Liver function-related indicators in the HCC and cirrhosis groups were significantly different from those in the control group (P<0.05). The expression level of p53 in HCC was significantly higher than that in the control group (P<0.05). The accuracy of diagnosis by using both p53 and CT was higher than the use of p53 or CT alone. CT can accurately measure the volume of each lobe of the liver, and p53 has important clinical values in the diagnosis of liver diseases. Thus, the reasonable combination of the two can effectively improve the diagnostic accuracy.

13.
Appl Microbiol Biotechnol ; 102(17): 7565-7575, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29955935

RESUMO

The anti-obesity drug GLP-1 has been proven to have an impact on central nervous system, while its extremely short half-life greatly limited its use. In this study, our group constructed two engineering strains MG1363-pMG36e-GLP-1 and VNP20009-pLIVE-GLP-1 to continuously express GLP-1, and supplementation of these strains, especially MG1363-pMG36e-GLP-1, had significantly restored the spatial learning and memory impairment of mice caused by LPS (p < 0.05), suppressed glia activation and Aß accumulation, and downregulated inflammatory expressions of COX-2, TLR-4, TNF-a, and IL-1ß. In addition, MG1363-pMG36e-GLP-1 had significantly blocked the translocation of NF-κB signal and inhibited the phosphorylation of redox-sensitive cytoplasmic signalings of MAPKs and PI3K/AKT. These data suggest that MG1363-pMG36e-GLP-1 could be used as a safe and effective nonabsorbed oral treatment for neuroinflammation-related diseases such as Alzheimer's disease (AD).


Assuntos
Bactérias/genética , Peptídeo 1 Semelhante ao Glucagon/genética , Transtornos da Memória/terapia , Microrganismos Geneticamente Modificados/metabolismo , Animais , Bactérias/metabolismo , Peptídeo 1 Semelhante ao Glucagon/administração & dosagem , Peptídeo 1 Semelhante ao Glucagon/metabolismo , Lipopolissacarídeos , Transtornos da Memória/induzido quimicamente , Camundongos , Microrganismos Geneticamente Modificados/genética
14.
Int J Biol Macromol ; 118(Pt A): 320-326, 2018 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-29920367

RESUMO

Fungal polysaccharides are mainly taken orally, so it is very important to study the absorption mechanism of which in vivo for understanding its biological activities. In this work, a polysaccharide fraction (GLP) was purified from G. lucidum by water extraction and alcohol precipitation. Physicochemical characterization indicated that GLP had a molecular weight of 108 kDa and consisted of glucose (35.56%), xylose (40.11%), rhamnose (16.45%) and l-arabinose (7.88%) in a molar radio of 4.51: 5.09: 2.88: 1.0. GLP was taken by Caco-2 cells through the pathway of macropinocytosis and it was a well-absorbed biological macromolecule testified by Papp value. Sugar transporters (SGLT1 and GLUT2) played different roles at different absorption stages of GLP, and the absorption of GLP during digestion in mice model was a time-dependent process. All these results could contribute to explore the absorption mechanism of polysaccharides in body.


Assuntos
Pinocitose/efeitos dos fármacos , Polissacarídeos/química , Reishi/química , Animais , Arabinose/química , Células CACO-2 , Glucose/química , Humanos , Camundongos , Peso Molecular , Polissacarídeos/administração & dosagem , Polissacarídeos/isolamento & purificação , Ramnose/química , Água/química , Xilose/química
15.
J Asian Nat Prod Res ; 20(5): 431-438, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29589484

RESUMO

Three new secoiridoids, nuezhenelenoliciside (1), isojaslanceoside B (2), 6'-O-trans-cinnamoyl-secologanoside (3), were isolated from the dried fruits of Ligustrum lucidum. Their structures were elucidated by comprehensive spectroscopic analysis. Among them, 1 featured a rare rearrangement product of secoiridoid, which underwent the cleavage of chemical bond between C-1 and O-2, and the reformation of a new iridoid ring between C-8 and O-2. In addition, all compounds were tested for their osteogenic activity on pre-osteoblastic MC3T3-E1 cells. As a result, 1 and 3 exhibited potent effects on promoting cell proliferation of pre-osteoblast cells.


Assuntos
Iridoides/química , Iridoides/farmacologia , Ligustrum/química , Células 3T3 , Animais , Calcificação Fisiológica/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Camundongos , Osteoblastos/efeitos dos fármacos , Osteogênese/efeitos dos fármacos
16.
ACS Appl Mater Interfaces ; 10(11): 9197-9202, 2018 Mar 21.
Artigo em Inglês | MEDLINE | ID: mdl-29443494

RESUMO

The survival of Staphylococcus aureus inside phagocytes is considered to be the sticking point of long-term chronic inflammation. Here, we fabricate peptide-chlorophyll-based photodynamic therapy (PDT) agents with "sandwich" dimeric structure to enhance the PDT effect and active targeting property to eliminate intracellular infections, which could be seen as prospective antibacterial agents for inflammation.


Assuntos
Staphylococcus aureus , Antibacterianos , Espaço Intracelular , Macrófagos , Fotoquimioterapia , Fármacos Fotossensibilizantes , Estudos Prospectivos , Infecções Estafilocócicas
17.
Clin Exp Hypertens ; 40(4): 312-317, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29048932

RESUMO

BACKGROUND: Increased arterial stiffness is an independent cardiovascular risk factor in smokers or patients with diabetes mellitus and hypertension. Cardio-ankle vascular index (CAVI) is an index of arterial stiffness and atherosclerosis. One of the most important risk factors of the causes of atherosclerosis is dyslipidemia(DLP). However, there was a little research about which influence factors such as: hypertension, diabetes mellitus, and smoking could contribute to the atherosclerosis in the subjects withDLP. METHODS: A total of 649 subjects with DLP (Male328/Female321) from Vascular Medicine of Peking University Shougang Hospital were examined, with a median age of 66 and 5-95 percentile range 47.0-83.5 years. Fasting plasma glucose (FPG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), triglyceride (TG) were analyzed by colorimetric enzymatic assays with the use of an auto analyzer (HITACHI-7170, Hitachi, Tokyo, Japan).CAVI was measured by VS-1000 apparatus. RESULTS: CAVI correlated significantly with age (p<0.001), Systolic (p<0.001) blood pressure(BP), Total cholesterol (p<0.001), LDL-cholesterol (p<0.001),Triglycerides (p<0.001) . There was no significant difference in CAVI between smokers and non-smokers (p = 0.08) and between statin-treated subjects than in those without statins (p = 0.247). CAVI was significantly higher in subjects with hypertension than in the normotensive group (p<0.001) and in mellitus subjects than in those without mellitus (p<0.001);however, CAVI values adjusted for age was higher only in hypertension than in the normotensive group (p<0.001). CONCLUSIONS: Our study demonstrated that CAVI value in DLP patients is not significantly affected by diabetes mellitus and smoking, but is increased by hypertension.


Assuntos
Diabetes Mellitus/fisiopatologia , Dislipidemias/fisiopatologia , Hipertensão/fisiopatologia , Fumar/fisiopatologia , Rigidez Vascular , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Tornozelo , Glicemia/metabolismo , Pressão Sanguínea , HDL-Colesterol/sangue , LDL-Colesterol/sangue , Dislipidemias/tratamento farmacológico , Feminino , Humanos , Inibidores de Hidroximetilglutaril-CoA Redutases/uso terapêutico , Japão , Masculino , Pessoa de Meia-Idade , Análise de Onda de Pulso , Fatores de Risco , Triglicerídeos/sangue
18.
Clin Exp Hypertens ; 40(5): 487-494, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29035100

RESUMO

Lifestyle modifications are recommended as the initial treatment for high blood pressure. The influence of dyslipidemia might be via moderate arterial stiffness, which results in hypertension and cardiovascular disease. We used data from a subgroup of the lifestyle, level of serum lipids/carotid femoral-pulse wave velocity (CF-PWV) Susceptibility BEST Study, a population-based study of community-dwelling adults aged 45-75 years. The serum lipid level and CF-PWV were measured at baseline, and lifestyle such as smoking status, sleeping habits, and the level of oil or salt intake was determined with the use of a validated questionnaire during follow-up. Arterial stiffness was determined as CF-PWV using an electrocardiogram after a mean follow-up of 4.4 years. Regression coefficients (95% CIs), adjusted for demographics, risk factors, cholesterol, and triglycerides (TGs), were calculated by linear regression. Logistic regression analysis was used to identify the association between the variables with CF-PWV independently. In the results, glucose and total cholesterol (TC) were associated with higher CF-PWV (p = 0.000) and lower-destiny lipoprotein was associated with lower CF-PWV (p = 0.001) after adjustments for age, sex, mean arterial pressure, and heart rate. There were significant associations observed for current salt intake in relation to CF-PWV (p-trend = 0.038) without adjustment. This association was retained after adjustments for covariates and had statistical significance (p-trend = 0.048) in model 3, which adjusted age, sex, baseline CF-PWV, mean arterial pressure, heart rate waist circumference, education, smoking status, physical activity, diabetes mellitus (DM), heart disease, high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, TGs, antihypertensive medicine, nitrate medicine, and antiplatelet medicine. Linear regression showed statistically significant associations between LDL and CF-PWV in the fully adjusted models (model 1 p = 0.010, model 2 p = 0.020, model 3 p = 0.017). Logistic regression analysis showed that CF-PWV was independently associated with age (p = 0.000), TC (p = 0.000), TGs (p = 0.000), and homo-cysteine (p = 0.000), and their odds ratios were 0.781, 3.424, 0.075, and 1.046, respectively. Our results showed a positive association between LDL and arterial stiffness, and suggested that less smoking status, sleeping disorder, and salt intake were associated with less arterial stiffness.


Assuntos
LDL-Colesterol/sangue , Hipertensão/fisiopatologia , Estilo de Vida , Rigidez Vascular , Idoso , Pressão Arterial , Glicemia/metabolismo , Artérias Carótidas/fisiologia , Colesterol/sangue , HDL-Colesterol/sangue , Ensaios Clínicos como Assunto , Feminino , Artéria Femoral/fisiologia , Seguimentos , Frequência Cardíaca , Homocisteína/sangue , Humanos , Estudos Longitudinais , Masculino , Pessoa de Meia-Idade , Análise de Onda de Pulso , Fatores de Risco , Transtornos do Sono-Vigília/fisiopatologia , Fumar/fisiopatologia , Cloreto de Sódio na Dieta/administração & dosagem , Triglicerídeos/sangue
19.
Clin Exp Hypertens ; 40(3): 274-280, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29227169

RESUMO

BACKGROUND: Carotid intima-media thickness (cIMT) is a significant early prediction signal for preclinical atherosclerosis. Plasma homocysteine (Hcy) level is an independent risk factor for cardiovascular diseases. Pulse wave velocity (PWV), an index of arterial stiffness (AS), has showed its value in the evaluation of AS in vascular-related diseases. The data regarding the relationships between cIMT and other indices of vascular damage are limited and partly controversial. We aim to investigate the relationships between cIMT and other indices of vascular damage such as Hcy and carotid femoral PWV (CF-PWV). METHODS: In total,1188 (male/female 514/674) subjects with hypertension from Vascular Medicine of Peking University Shougang Hospital were enrolled into our study. The patients with hypertension were measured bilateral cIMT by B-mode ultrasound and measured plasma levels of Hcy, total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL), C-reactive protein (CRP), glucose, and other biomarkers. Plaques were detected in 78% of the study patients. Plasma Hcy (p < 0.001), CRP (p <  0.001), LDL (p < 0.001), HDL (p = 0.008), TC levels (p < 0.001), and CF-PWP (p < 0.001) were significantly greater in hypertensive patients with carotid plaques than patients without. The carotid plaque detection rate (p < 0.001) and bilvertebral artery max velocity (p < 0.05) were significantly different in quartiles of plasma Hcy levels. R-IMT correlated significantly with HCY, high sensitive CRP (hs-CRP), CF-PWV, and R-vertebral artery intimal diameter. Age (p < 0.001) and CF-PWV (p < 0.05) predicted the presence of carotid plaques independently in the logistic regression. However, Hcy did not independently predict the presence of carotid plaques in the logistic regression. CONCLUSION: Increased carotid femoral PWV and elevated plasma Hcy levels are associated with subclinical carotid disease in hypertensive patients. CF-PWV independently predicted subclinical carotid plaque in the patients with hypertensive. While, there is insufficient evidence for Hcy in predicting plaque incidence.


Assuntos
Espessura Intima-Media Carotídea , Homocisteína/sangue , Hipertensão/sangue , Placa Aterosclerótica/sangue , Placa Aterosclerótica/diagnóstico por imagem , Rigidez Vascular , Fatores Etários , Idoso , Doenças Assintomáticas , Biomarcadores/sangue , Proteína C-Reativa/metabolismo , Colesterol/sangue , Estudos Transversais , Feminino , Humanos , Hipertensão/complicações , Hipertensão/fisiopatologia , Lipoproteínas HDL/sangue , Lipoproteínas LDL/sangue , Masculino , Pessoa de Meia-Idade , Placa Aterosclerótica/complicações , Placa Aterosclerótica/fisiopatologia , Análise de Onda de Pulso , Fatores de Risco
20.
Mol Med Rep ; 17(2): 2764-2770, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29207096

RESUMO

To minimize the waste of active ingredients present in herb residues, in the present study, probiotics of Bacillus subtilis, Aspergillus oryzae and Lactobacillus plantarum M3 were selected to reuse herb residues from Jianweixiaoshi tablets, and the therapeutic effects of the herb residue fermentation supernatant were evaluated using a spleen­deficient mouse model. The results of the present study indicated that the fermentation supernatant may effectively improve the immunity of mice, as measured by body weight, spleen and thymus index, and inflammatory cytokines, including interleukin (IL)­2, IL­4 and interferon­Î³. The viable cell count and denaturing gradient gel electrophoresis results indicated that the fermentation supernatant markedly enhanced bacterial diversity and the number of lactobacilli in mouse intestines. Therefore, the combination of the Jianweixiaoshi herb residue and probiotics provided a novel method to reuse herb residues and may in the future contribute to the treatment of spleen deficiency.


Assuntos
Meios de Cultivo Condicionados/farmacologia , Fermentação , Probióticos/metabolismo , Animais , Bactérias/metabolismo , Biomarcadores , Citocinas/metabolismo , Mediadores da Inflamação/metabolismo , Lactobacillus/metabolismo , Camundongos , Esplenectomia , Comprimidos
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