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1.
Food Chem ; 366: 130642, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34304135

RESUMO

Morchella sp. is a typical edible fungus. However, its unique natural products, especially bioactive composition, are rarely reported. In this study, an untargeted metabolomics approach combined with multivariate statistical analysis was employed to screen the differential metabolites in Morchella sp. compared to four other edible fungi. Among the screened 50-top differential metabolites, 18 compounds involving fatty acids and peptides showed higher content in Morchella sp.. A unique peptide of desferriferricrocin was identified. Subsequently, molecular networking analysis revealed three novel homologous compounds of desferriferricrocin, which were annotated as desferriferrichrome, desferriferrichrome C, and a new homolog not previously reported. These results were further verified by quantitative analysis. To the best of our knowledge, this is the first report of four peptides in Morchella sp..


Assuntos
Ascomicetos , Metabolômica , Cromatografia Líquida de Alta Pressão
2.
BMJ Open ; 11(10): e046252, 2021 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-34615674

RESUMO

OBJECTIVE: To synthesise the available scientific evidence on the effects of combined exercise on glycaemic control, weight loss, insulin sensitivity, blood pressure and serum lipids among patients with type 2 diabetes (T2D) and concurrent overweight/obesity. DESIGN AND SAMPLE: PubMed, EMBASE, Web of Science, the Cochrane library, WANFANG, CNKI, SinoMed, OpenGrey and ClinicalTrials.gov were searched from inception through April 2020 to identify randomised controlled trials (RCTs) that reported the effects of combined exercise in individuals with T2D and concurrent overweight/obesity. METHODS: Quality assessment was performed using the Cochrane Collaboration's risk of bias tool. The mean difference (MD) with its corresponding 95% CI was used to estimate the effect size. Meta-analysis was performed using Review Manager V.5.3. RESULTS: A total of 10 RCTs with 978 participants were included in the meta-analysis. Pooled results demonstrated that combined exercise significantly reduced haemoglobin A1c (MD=-0.16%, 95% CI: -0.28 to -0.05, p=0.006); body mass index (MD=-0.98 kg/m2, 95% CI: -1.41 to -0.56, p<0.001); homeostasis model assessment of insulin resistance (MD=-1.19, 95% CI: -1.93 to -0.46, p=0.001); serum insulin (MD=-2.18 µIU/mL, 95% CI: -2.99 to -1.37, p<0.001) and diastolic blood pressure (MD=-3.24 mm Hg, 95% CI: -5.32 to -1.16, p=0.002). CONCLUSIONS: Combined exercise exerted significant effects in improving glycaemic control, influencing weight loss and enhancing insulin sensitivity among patients with T2D and concurrent overweight/obesity.

3.
Hematology ; 26(1): 809-817, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34602023

RESUMO

INTRODUCTION: Cytokine release syndrome (CRS) is a common complication after T-replete HLA haploidentical hematopoietic cell transplantation (haplo-HCT) with PTCy. We aim to assess the incidence, severity, and impact of CRS on clinical outcomes of patients who received haplo-HCT using Beijing Protocol. METHODS: This was a single-enter retrospective analysis of 286 subjects who received haplo-HCT with Antithymocyte Globulin (ATG). RESULTS: We identified 147/268 (54.9%) patients who developed CRS, grade 1 CRS (32.5%) and grade ≥2 CRS (22.4%). Eight patients developed severe CRS. The incidence and severity of CRS did not show significant discrimination among patients who received different doses of ATG. By multivariable analysis, age and the disease status at transplantation were significantly associated with the occurrence of CRS (p =.000 and p = .021). In the univariate analysis for the severity of CRS, compared with CRS grade ≥2, patients with CRS grade 0-1 had higher 1-year overall survival (OS) (p = .009). The cumulative incidence of 100-day grades II-IV acute GVHD was 12.4%. The incidence did not show significant differences between patients with CRS or not. The devolvement of CRS is associated with worse OS, inferior disease-free survival, and higher nonrelapse mortality significantly. But the result appeared to be limited to patients in uncomplete remission status before transplantation. DISCUSSION AND CONCLUSIONS: CRS is less frequent and milder with a protocol based on ATG. CRS can potentially affect the outcomes after haplo-HCT especially for patients in an uncomplete remission. Prospective clinical trials are needed to provide an appropriate scheme for CRS prophylaxis.

4.
Arch Gerontol Geriatr ; 98: 104547, 2021 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-34634494

RESUMO

OBJECTIVES: This study aimed to summarize the effects of exercise on the brain structure and function measured by imaging and electroencephalography methods in people with mild cognitive impairment and Alzheimer's disease. METHODS: We systematically searched different electronic databases, including PubMed, Web of Science, Embase, Cochrane Central Register of controlled trials, SPORTDiscus, PsycINFO up to September 2021. Randomized controlled trials with exercise interventions in people with mild cognitive impairment and Alzheimer's disease were included. The outcomes included all the structural and functional brain changes measured by neuroimaging and electroencephalography approaches. All the outcomes were summarized and tabulated. The protocol has been registered on the PROSPERO (CDR42020192579). RESULTS: Nineteen articles from 12 trials were included. Most studies focused on people with mild cognitive impairment and revealed positive effects on the volumes of hippocampus and cingulate cortex, the resting state functional connectivity between the hippocampus and other brain regions, the brain activity in several regions, the cerebral blood flow, electroencephalogram, and P300 latency and amplitude. Different types of exercise could produce distinct effects and intensity was a factor affecting the effects of aerobic and resistance exercise interventions. Meanwhile, limited studies paid attention to people with Alzheimer's disease and showed insignificant results. CONCLUSIONS: Exercise has positive effects on the brain structures and functions in people with mild cognitive impairment, while for people with Alzheimer's disease, limited studies revealed insignificant effects.

5.
Appl Opt ; 60(27): 8384-8389, 2021 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-34612937

RESUMO

A cantilever deflection fiber-optic sensor based on chirped fiber grating (CFG) Fabry-Perot (FP) cavity had been proposed and experimental demonstrated. Two CFBGs with the same chirped coefficient direction and grating parameters are written in one single-mode fiber by UV mask exposure to form the CFG-FP cavity. The central wavelength of two CFGs is 1549.6072 nm, the 3 dB bandwidth is 2.9897 nm, and the physical cavity length of two CFGs is 1 cm. The grating region of two CFGs are straightness fixed on a cantilever beam, forming a sensor. Then the optical properties of this sensor are tested with different stresses at different positions of the cantilever beam. The experimental results show that this sensor can obtain linear displacement of a cantilever beam, overcoming the abilities of the FBG sensor, which only performed point measurement defect. The wavelength drift sensitivity of the sensor is 2.31 pm/g, and linearity is 0.99916. This sensor has great application value in the precise measurement of cantilever beam type and two-dimensional scale strain.

6.
Food Chem ; 369: 130969, 2021 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-34500206

RESUMO

Modified atmosphere packaging (MAP) can inhibit microbial growth and prolong shelf life of fresh-cut cucumbers. This study compared the effects of different packaging gases on the growth of E. coli O157:H7 and sensory characteristics of fresh-cut cucumbers. Changes in key movement, adhesion, and oxidative stress genes expression of strain under optimal MAP and air were determined. Cell population density, the extracellular carbohydrate complex content and expression of curli fimbriae were evaluated. Results revealed that the growth of E. coli O157:H7 in fresh-cut cucumbers could be effectively inhibited under MAP (atmosphere = 2% O2, 7% CO2, 91% N2), and better maintained the sensory characteristics. Furthermore, the inhibition mechanism was revealed by inhibiting the expression of movement (fliC), adhesion (eaeA) and oxidative stress (rpoS and sodB) genes in E. coli O157:H7, reducing biofilm formation, extracellular carbohydrate production and curli fimbriae expression. Proper MAP can maintain the quality and safety of fresh-cut cucumbers.

7.
Food Chem ; 369: 130939, 2021 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-34469843

RESUMO

The purpose of this study was to investigate the dynamic change in volatile components during the yellowing process. The volatile components were analyzed by headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS) and the critical enzyme activities were determined by commercial kits. The results revealed that 29 signal peaks for 20 compounds were identified, which were quantified in all samples: 1 furan, 1 ester, 15 aldehydes, 3 ketones, and 9 alcohols. The contents of most of these compounds increased first and then decreased at 36 h, which were basically consistent with the enzyme activities of LOX, HPL, ADH and AAT. Subsequently, principal component analysis (PCA) results clearly showed that the fresh-cut yams for different yellowing processes were well distinguished by the volatile compounds. These results showed that the potential of HS-GC-IMS-based approaches to evaluate the volatile compound profiles of fresh-cut yam at different stages in the yellowing period.

8.
Artigo em Inglês | MEDLINE | ID: mdl-34558886

RESUMO

PbI2 is a commonly used passivator for defect passivation in perovskite solar cells (PSCs). However, the poor conductivity nature of PbI2 may limit the further improvement of device performance. Here, we report a radical form of PbI2 with high conductivity to passivate defects for efficient PSCs through a combination of N,N,N',N'-tetramethylbenzidine (TMB). When PbI2 is combined with TMB, 4 orders of magnitude higher conductivity will be achieved owing to the formation of a TMB-PbI2 radical. As a result, the device performance is impressively increased from 20.48 to 22.63%. In addition, the device stability is also greatly improved and 95% of the initial efficiency is retained after aging at 85 °C for 600 h.

9.
Neurotox Res ; 2021 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-34499332

RESUMO

Aluminum is a widespread environmental neurotoxicant that can induce Alzheimer's disease (AD)-like damage, such as neuronal injury and impairment of learning and memory. Several studies have shown that aluminum could reduce the synaptic plasticity, but its molecular mechanism remains unclear. In this study, rats were treated with aluminum maltol (Al(mal)3) to establish a toxic animal model and PMA was used to interfere with the expression of PKC. The Morris water maze and open field test were used to investigate the behavioral changes of the rats. Western blotting and RT-PCR were used to detect the expression levels of NMDAR subunits, PKC and CaMKII. The results showed that Al(mal)3 damaged learning and memory function and reduced anxiety in rats. During this process, the expression of PKC was downregulated and it inhibited the expression of NMDARs through the phosphorylation of CaMKII.

10.
Lancet Gastroenterol Hepatol ; 6(11): 914-921, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34555347

RESUMO

BACKGROUND: The use of magnetically controlled capsules for gastroscopy is in the early stages of clinical adoption. We aimed to evaluate the safety and efficacy of a fully automated magnetically controlled capsule endoscopy (FAMCE) system in clinical practice for gastroscopy and small bowel examination. METHODS: We did a prospective, comparative study to evaluate the safety and efficacy of FAMCE. Patients from two hospitals in Chongqing, China were consecutively enrolled. Eligible participants were aged 18-80 years with suspected gastric pathology and no previous surgery. Participants underwent FAMCE for screening of gastric lesions, then conventional transoral gastroscopy 2 h later, and stomach examination results were compared. The primary outcome was the rate of complete detection of gastric anatomy landmarks (cardia, fundus, body, angulus, antrum, and pylorus) by FAMCE. Secondary outcomes were the time required for gastric completion by FAMCE, the rate of detection of gastric lesions by FAMCE compared with conventional transoral gastroscopy, and the rate of complete small bowel examination. Adverse events were also evaluated. The study was registered in the Chinese Clinical Trial Registry, ChiCTR2000040507. FINDINGS: Between May 12 and Aug 17, 2020, 114 patients (mean age 44·0 years [IQR 34·0-55·0]; 63 [55%] female) were enrolled. The rate of complete detection of gastric anatomical structures by FAMCE was 100% (95% CI 99·3-100·0). The concordance between FAMCE and conventional transoral gastroscopy was 99·61% (99·45-99·78). The mean completion time of a gastroscopy with FAMCE was 19·17 min (SD 1·43; median 19·00, IQR 19·00-20·00), compared with 5·21 min (2·00; 5·18, 3·68-6·45) for conventional transoral gastroscopy. In 114 enrolled patients, 214 lesions were detected by FAMCE and conventional transoral gastroscopy. Of those, 193 were detected by both modalities. FAMCE missed five pathologies (four cases of gastritis and one polyp), whereas conventional transoral gastroscopy missed 16 pathologies (12 cases of gastritis, one polyp, one fundal xanthoma, and two antral erosions). FAMCE was able to provide a complete small bowel examination for all 114 patients and detected intestinal lesions in 50 (44%) patients. During the study, two (2%) patients experienced adverse events. No serious adverse events were recorded, and there was no evidence of capsule retention. INTERPRETATION: The performance of FAMCE is similar to conventional transoral gastroscopy in completion of gastric examination and lesion detection. Furthermore, it can provide a complete small bowel examination. Therefore, FAMCE could be effective method for examination of the gastrointestinal tract. FUNDING: Chinese National Key Research and Development Program.

11.
Ecotoxicol Environ Saf ; 225: 112785, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34544021

RESUMO

Neonicotinoids are among the most widely used insecticides worldwide, and as such, have garnered increasing attention from the scientific community in regards to their potentially negative environmental impacts. Recently, the degradability of neonicotinoid in soil has gained more attentions. However, what role soil microbes play in this degradation remains vastly underexplored. In this study, we compared the capacity of soil microbes sampled from different geographic regions and fields to degrade the neonicotinoid insecticide imidacloprid. Additionally, the composition of microbiota having low, middle, and high degradation activity was analyzed via high throughput sequencing. Correlations between microbiota composition and degradation activities were analyzed and reconfirmed. The results showed that the composition of soil microbiota and their degradation activity (ranged from zero to 96.25%) varied significantly between soil samples from different geographic locations. Correlation analysis showed that Paracoccus and Achromobacter bacteria were positively correlated with high degradation activity. Imidacloprid degradation experiments using these bacteria showed that Achromobacter sp. alone exhibited degradation activity reaching and sustaining 100% by day 20 while Paracoccus sp. did not. However, combining these bacteria resulted in increased degradation activity which reached 100% at day 15 relative to that achieved by Achromobacter sp. alone. This study demonstrated the capacity of soil microbes to degrade imidacloprid, and identified two promising bacterial candidates that could be potentially used in future to reduce imidacloprid accumulation in soils.


Assuntos
Achromobacter , Inseticidas , Paracoccus , Poluentes do Solo , Bactérias , Biodegradação Ambiental , Neonicotinoides , Nitrocompostos , Solo , Microbiologia do Solo
12.
Mol Biol Rep ; 48(8): 6075-6083, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34374892

RESUMO

BACKGROUND: Endometrial cancer is generally one of the most evident malignant tumours of the female reproductive system, and the mechanisms underlying its cell proliferation and apoptosis are key to research in gynaecological oncology. In the paper, the in-depth molecular mechanism by which DJ-1 protein regulates the proliferation and apoptosis of Ishikawa cells was investigated. METHODS AND RESULTS: DJ-1 knockdown and overexpressing Ishikawa stable cell lines were established by lentiviral transduction. The levels of DJ-1 and noncanonical NF-κB signaling key proteins were evaluated by Western blotting. Cell counting kit-8 (CCK-8) and flow cytometry were applied to analyze the cell viability and apoptosis. Co-immunoprecipitation experiment was utilized to assess the DJ-1-Cezanne interaction. The results showed that DJ-1 overexpression conferred apoptosis resistance and high proliferation on Ishikawa cells, while DJ-1 knockdown in Ishikawa cells produced the opposite results. These findings again suggested that DJ-1 inhibits the apoptosis and promotes the proliferation of Ishikawa cells. More crucially, further data showed that the noncanonical NF-κB activation was required for the regulation of Ishikawa cell proliferation and apoptosis by DJ-1. Meanwhile, it was found that noncanonical NF-κB pathway may be activated by DJ-1 interacting with and negatively regulating Cezanne in Ishikawa cells. CONCLUSIONS: Overall, this work revealed that DJ-1 associates with and negatively regulates Cezanne and consequently triggers the noncanonical NF-κB activation, thereby regulating Ishikawa cell proliferation and apoptosis.

13.
Neurosci Lett ; 762: 136149, 2021 09 25.
Artigo em Inglês | MEDLINE | ID: mdl-34352339

RESUMO

BACKGROUND: Cognitive impairment (CI) is important for the prognosis of Parkinson's disease (PD). Early prediction whether and when cognitive decline from normal cognition (NC) will occur is crucial for preventing or delaying the progression timely. The current study aimed to provide a personalized risk assessment of CI by using baseline information and establishing a multi-predictor nomogram. METHODS: 108 patients with PD were collected from the Parkinson's Progression Markers Initiative (PPMI), of whom 58 had progressed to CI and 50 remained NC during 5-year follow up. Radiomics signatures were obtained by using least absolute shrinkage and selection operator (LASSO) Cox regression algorithm. Clinical factors and laboratory biomarkers were selected by multivariate Cox regression analysis. The combined model of radiomics signatures and clinical risk factors was developed by a multivariate Cox proportional hazard model. A multi-predictor nomogram derived from the combined model was established for individualized estimation of time to progress (TTP) of CI. We analyzed the risk of two subgroups of the combined model by Kaplan-Meier (KM) analysis. RESULTS: The combined model showed the best performance with a C-index of 0.988 and 0.926 in the training and validation datasets. KM analysis verified significant TTP of CI (P<0.05) between two subgroups stratified by the cutoff value (-0.058). CONCLUSION: The combined model and its multi-predictor nomogram can be used to perfectly and individually predict the TTP of CI for patients with PD. Stratification of PD will benefit its timely clinical intervention and the delay and prevention of CI.

14.
J Microbiol Biotechnol ; 31(10)2021 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-34409950

RESUMO

Herein, we cloned and expressed an endo-ß-1,4-glucanase gene (celA1805) from Bacillus subtilis B111 in Escherichia coli. The recombinant celA1805 contains a glycosyl hydrolase (GH) family 8 domain and shared 76.8% homology with endo-1,4-ß-glucanase from Bacillus sp. KSM-330. Results showed that the optimal pH and temperature of celA1805 were 6.0 and 50◦C, respectively, and it was stable at pH 3-9 and temperature ≤50°C. Metal ions slightly effected enzyme activity, but chemical agents generally inhibited enzyme activity. Moreover, celA1805 showed a wide substrate specificity to CMC, barley ß-Glucan, lichenin, chitosan, PASC and Avcel. The Km and Vmax values of celA1805 were 1.78 mg/mL and 50.09µmol/min/mg. When incubated with cellooligosaccharides ranging from cellotriose to cellopentose, celA1805 mainly hydrolyzed cellotetrose (G4) and cellopentose (G5) to cellose (G2) and cellotriose (G3), but hardly hydrolyzed cellotriose. The concentration of reducing sugars saccharified by celA1805 from wheat straw, rape straw, rice straw, peanut straw, and corn straw were increased by 0.21, 0.51, 0.26, 0.36, and 0.66 mg/mL, respectively. The results obtained in this study suggest potential applications of celA1805 in biomass saccharification.

15.
Artigo em Inglês | MEDLINE | ID: mdl-34436647

RESUMO

PURPOSE: To assess the longitudinal change in components of astigmatism from age 4 to 7 years and its association with axial length-corneal radius ratio (AL/CR). METHODS: Children born between September 2011 and August 2012 in Yuhuatai District of Nanjing were invited to participate in the Nanjing Eye Study for a comprehensive eye examination annually since 2015. The data presented in this paper were obtained in 2016, 2017, and 2019. At each study encounter, noncycloplegic autorefraction and ocular biometric parameters were measured. Changes of total astigmatism (TA), corneal astigmatism (CA), anterior corneal astigmatism (ACA), residual astigmatism (RA), and internal astigmatism (IA) were analyzed in clinical notation (Cyl) and vector notation (J0, J45). RESULTS: Nine hundred fifty-four children (mean ± standard deviation of baseline age: 4.63 ± 0.29 years, 53.7% boys) had complete data and were included in this study. Mean slopes of longitudinal changes in Cyl notation were significantly negative for TA, CA, and ACA, but positive for IA. TA, CA, ACA, and RA of J0 notation had a shift toward increasing with-the-rule (WTR) astigmatism and/or decreasing against-the-rule (ATR) astigmatism. TA of J45 notation showed an increase in astigmatism at axis 135° and/or a decrease in astigmatism at axis 45°, while CA and ACA of J45 notation showed an opposite change. Longitudinal changes in ACA and IA were negatively correlated in J0 notation, but not in J45 notation. Based on compensation factor (CF, defined as the minus ratio of IA and ACA), the compensation proportions for J0 in varying degrees (CF: 0.1-2) in 2016, 2017, and 2019 were 91.3%, 93.5%, and 90.0%, respectively, while these for J45 were 74.9%, 76.5%, and 34.6%, respectively. Higher AL/CR increase was associated with less decrease or more increase in CA and ACA of Cyl notation, and a shift toward increasing WTR and/or decreasing ATR in these of J0 notation. CONCLUSIONS: The compensatory role of IA was persistent and prominent from 4 to 7 years old for J0 notation in Chinese young children. The progression of AL/CR was correlated with astigmatism originated from the cornea.

16.
Cancer Invest ; 39(8): 675-684, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34241563

RESUMO

The major problems with cancer therapy are drug-induced side effects. There is an urgent need for safe anti-tumor drugs. Artemisinin is a Chinese herbal remedy for malaria with efficacy and safety. However, several studies reported that artemisinin causes neurotoxicity and cardiotoxicity in animal models. Recently, nanostructured drug delivery systems have been designed to improve therapeutic efficacy and reduce toxicity. Artemisinin has been reported to show anticancer properties. The anticancer effects of artemisinin appear to be mediated by inducing cell cycle arrest, promoting ferroptosis and autophagy, inhibiting cell metastasis. Therefore, the review is to concentrate on mechanisms and molecular targets of artemisinin as anti-tumor agents. We believe these will be important topics in realizing the potential of artemisinin and its derivatives as potent anticancer agents.


Assuntos
Antineoplásicos/uso terapêutico , Artemisininas/uso terapêutico , Neoplasias/tratamento farmacológico , Antineoplásicos/farmacologia , Artemisininas/farmacologia , Humanos
17.
J Adv Nurs ; 2021 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-34324218

RESUMO

AIMS: To synthesize evidences on smartphone application-based intervention and determine its effectiveness on glycaemic control, self-management behaviours, psychological well-being, quality of life and cardiometabolic risk factors. DESIGN: A systematic review and meta-analysis of randomized controlled trials (RCTs). DATA SOURCES: Major English and Chinese electronic databases were searched from January 2008 to January 2021, including PubMed, Web of Science, Embase, Cochrane Central Register of Controlled Trials, Google Scholar, China National Knowledge Infrastructure (CNKI), Wanfang and Sinomed. REVIEW METHODS: RCTs were screened and selected if they used smartphone applications to support patients in the self-management of diabetes. Data extraction and methodological assessment were performed by two reviewers independently. Meta-analysis was performed to pool the intervention effect on outcomes of interest using RevMan 5.3. RESULTS: Across 19 included trials involving 2585 participants, smartphone application-based interventions were associated with a clinically and statistically significant reduction of glycated haemoglobin (HbA1c). Beneficial effects were also observed in participants' behavioural performance, especially in medication adherence. Intervention effects on psychological status, quality of life and cardiometabolic risk factors were nonsignificant. Subgroup analysis showed interactive approach with medium frequency or flexible facilitator-patient interaction induced a larger effect on HbA1c reduction. Besides, patients with baseline HbA1c ≥9% benefited more than those with HbA1c <9% from the use of smartphone applications. CONCLUSIONS: Smartphone application-based diabetes self-management intervention could optimize patients' glycaemic control and enhance participants' self-management performance. Further endeavour is required to examine the long-term effects and cost-effectiveness of smartphone application-based intervention before promoting the adoption and dissemination of such intervention. IMPACT: This review supports the potential of smartphone application-based intervention as effective approach to optimize glycaemic control and promote self-management engagement among patients with type 2 diabetes. Suggestions for future research and practice are provided and discussed.

18.
BMJ Open ; 11(7): e044518, 2021 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-34210722

RESUMO

OBJECTIVE: Rapid, accurate identification of patients with acute myocardial infarction (AMI) at high risk of in-hospital major adverse cardiac events (MACE) is critical for risk stratification and prompt management. This study aimed to develop a simple, accessible tool for predicting in-hospital MACE in Chinese patients with AMI. DESIGN: Retrospective review of deidentified medical records. SETTING: 38 urban and rural hospitals across diverse economic and geographic areas in China (Beijing, Henan Province and Jilin Province). PARTICIPANTS: 15 009 patients discharged from hospital with a diagnosis of AMI. MAIN OUTCOME MEASURE: The primary outcome was MACE occurrence during index hospitalisation. A multivariate logistic regression model (China AMI Risk Model, CHARM) derived using patient data from Beijing (n=7329) and validated with data from Henan (n=4247) and Jilin (n=3433) was constructed to predict the primary outcome using variables of age, white cell count (WCC) and Killip class. C-statistics evaluated discrimination in the derivation and validation cohorts, with goodness-of-fit assessed using Hosmer-Lemeshow statistics. RESULTS: The CHARM model included age (OR: 1.06 per 1-year increment, 95% CI 1.05 to 1.07, p<0.001), WCC (OR per 109/L increment: 1.10 (95% CI 1.07 to 1.13), p<0.001) and Killip class (class II vs class I: OR 1.34 (95% CI 0.99 to 1.83), p=0.06; class III vs class I: OR 2.74 (95% CI 1.86 to 3.97), p<0.001; class IV vs class I: OR 14.12 (95% CI 10.35 to 19.29), p<0.001). C-statistics were similar between the derivation and validation datasets. CHARM had a higher true positive rate than the Thrombolysis In Myocardial Infarction score and similar to the Global Registry of Acute Coronary Events (GRACE). Hosmer-Lemeshow statistics were 5.5 (p=0.703) for derivation, 41.1 (p<0.001) for Henan, and 103.2 for Jilin (p<0.001) validation sets with CHARM, compared with 119.6, 34.0 and 459.1 with GRACE (all p<0.001). CONCLUSIONS: The CHARM model provides an inexpensive, accurate and readily accessible tool for predicting in-hospital MACE in Chinese patients with AMI.


Assuntos
Infarto do Miocárdio , China/epidemiologia , Hospitais , Humanos , Infarto do Miocárdio/epidemiologia , Estudos Retrospectivos , Medição de Risco , Fatores de Risco
19.
BMC Ophthalmol ; 21(1): 283, 2021 Jul 21.
Artigo em Inglês | MEDLINE | ID: mdl-34289821

RESUMO

BACKGROUNDS: Myopia has become a global public health problem. Children with early onset of myopia are at particular risk of complications associated with myopia. Younger children and children with greater initial myopic refractive errors are at a greater risk of myopia progression. Therefore, it is essential to identify subjects at high risk of developing myopia to facilitate myopia prevention in the early stage, especially during the preschool period. The purpose of this study was to determine whether premyopia and myopia in preschool children can be predicted by easily obtainable parameters. METHODS: Data was collected in a population-based cohort. Comprehensive examinations included height, weight, refraction, axial length (AL), and corneal radius of curvature (CR), with a follow-up of 2 years. Parental myopia history was obtained from a questionnaire. Myopia was defined as spherical equivalent (SE) ≤ - 0.50 D. Premyopia was defined as - 0.50 D < SE ≤ + 0.75 D. Multivariate linear regression models were fitted to determine the associations between these parameters at baseline and future SE. To predict premyopia and myopia, Cox proportional hazard regression analysis coupled with a nomogram was used. RESULTS: A total of 830 children (433 boys and 397 girls) were included (40.83 ± 3.43 months old at baseline). A significantly negative relationship was observed in the multivariate analysis between baseline AL, AL/CR, two myopic parents, and the future SE after adjusting for age and gender (coefficient = - 0.291, coefficient = - 5.791, coefficient = - 0.273, respectively, both p <  0.001). Higher baseline AL, AL/CR (hazard ratio (HR) = 4.916, HR = 2.979, respectively, comparing the top quartile with the bottom quartile, both p <  0.001) and two myopic parents (HR = 1.756, compared to no myopic parents, p = 0.001) were associated with a higher risk of future onset of premyopia. From the nomogram, AL/CR was found to have the most enormous effect on survival. Different baseline AL and AL/CR values (both Log Rank p <  0.001) had different survival curves. CONCLUSIONS: AL and AL/CR could be used as obtainable indicators for identifying subjects at high risk of developing premyopia and myopia in young preschool children.


Assuntos
Comprimento Axial do Olho , Miopia , Pré-Escolar , China/epidemiologia , Córnea , Progressão da Doença , Feminino , Humanos , Masculino , Miopia/epidemiologia , Refração Ocular
20.
Food Chem ; 364: 130423, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34198034

RESUMO

Oxidative stress in radish roots causes internal blue discoloration and decreases vegetable quality. Accordingly, the effects of different oxygen concentration treatment on this coloration during storage was investigated; 4-hydroxyglucobrassicin content (a precursor of the blue component); the reactive oxygen species (ROS) superoxide (O2-) and hydrogen peroxide (H2O2); the antioxidants ascorbic acid (AsA) and glutathione (GSH); and the activities and gene expression levels of the enzymes catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX), glutathione peroxidase (GPX), were monitored under normal and low-oxygen conditions. The results indicated that packaging radish roots under 10% O2 prevents blue discoloration by decreasing the activity and expression of the oxidant enzyme POD, increasing the levels of antioxidant and reducing substances, and upregulating antioxidant enzymes, all of which act to decrease the generation of ROS (O2- and H2O2).


Assuntos
Raphanus , Antioxidantes , Ascorbato Peroxidases/metabolismo , Catalase/genética , Catalase/metabolismo , Armazenamento de Alimentos , Peróxido de Hidrogênio , Estresse Oxidativo , Oxigênio , Raízes de Plantas/metabolismo , Raphanus/genética , Raphanus/metabolismo , Espécies Reativas de Oxigênio , Superóxido Dismutase/metabolismo
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