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1.
Front Oncol ; 11: 744064, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34722299

RESUMO

Background: The landscape of intratumor heterogeneity (ITH) is present from the tumor evolution. ITH is a promising clinical indicator, but the association between ITH and prognosis remains controversial. Therefore, a meta-analysis was performed to explore whether ITH can serve as a valuable prognostic indicator in solid tumors. Methods: All included studies were from PubMed, Embase, Cochrane, and Web of Science databases up to October 10, 2020. Studies based on ITH with available prognostic information were included. Three researchers independently completed study selection and data extraction following PRISMA guidelines. The random-effect model was used for synthesis. Hazard ratio (HR) and 95% confidence intervals (CI) were used with the endpoint defined by overall survival (OS), disease-specific survival (DFS), and progression-free survival (PFS). Results: A total of 9,804 solid tumor patients from 21 studies were included. Analysis of specific cancers in the TCGA database showed similar results based on different ITH assessment methods, which provided the logical support for data consolidation. Available evidence revealed a negative relationship between ITH and prognosis for a specific cancer (such as lung cancer). However, the OS results from 14 tumor types showed that high ITH associated with shorter survival time [HR 1.65 (95% CI, 1.42-1.91)]. PFS and DFS analyses showed similar results [HR 1.89 (95% CI, 1.41-2.54) and HR 1.87 (95% CI, 1.15-3.04)] in general. The status of tumor metastasis and sampling models were not the confounding factors. Conclusions: High ITH is associated with worse prognosis in many solid tumors in general although this association was absent for some cancers. ITH is expected to be a promising clinical prognostic factor for the improvement of assessment, treatment, and surveillance strategy.

2.
Exp Eye Res ; 213: 108810, 2021 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-34757002

RESUMO

Retinal arterial macroaneurysms are characterized by the acquired fusiform or saccular dilatations of the retinal artery. Angiotensin II (Ang II) is a major signal molecule of the renin-angiotensin system, which exerts a range of pathogenic actions that are relevant to retinal vascular abnormalities. We aimed to study the effect of Ang II on retinal vessels and explore its relationship with retinal aneurysmal disease. C57BL/6J male mice were administered Ang II at 1000 ng/kg/min for 28 days, and the mice given saline served as controls. The mice in the treatment group were treated once daily by gastric gavage of candesartan cilexetil (an antagonist of Ang II type 1 (AT1) receptor) at 100 mg/kg/day. The in vivo imaging of murine retinas was performed using fundus photography, optical coherence tomography, fluorescein angiography, and indocyanine green angiography at 7th, 14th, and 28th days of infusion. At the end of the infusion and treatment, the morphological changes were evaluated by histopathological examination and electron microscopy; the levels of related proteins in murine retinas were examined by antibody array and Western blot analyses. We found that Ang II infusion induced aneurysm formation in mice retina, which presented as either solitary aneurysms or retinal arterial beading. The aneurysm formation was often accompanied with vessel leakage. Moreover, Ang II infusion itself may result in increased vascular permeability and ganglion cell and inner plexiform layer thickening. The blockade of AT1 receptors by systemic administration of candesartan cilexetil alleviated the Ang II-induced retinal vasculopathy. The protein level analysis further showed that Ang II upregulated IL-1ß, PDGFR-ß, and MMP-9 expression, and the expression of IL-1ß could be inhibited by AT1 receptor antagonist. Our study provides evidence that Ang II is a crucial factor in retinal aneurysm formation and vessel leakage. It is probably the combined effect of Ang II on vessel inflammatory response, pericyte function, and extracellular matrix remodeling that predisposes the retinal arterial wall to aneurysm formation and blood-retinal barrier breakdown.

3.
Int Ophthalmol ; 2021 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-34743256

RESUMO

PURPOSE: To introduce and evaluate a modified therapeutic strategy for idiopathic macular holes (IMH). METHODS: A retrospective review of patients with diagnosis of IMH from July 2016 to January 2020 at Peking Union Medical College Hospital. These patients were managed strictly according to our therapeutic strategy. Their comprehensive clinical data were collected and analyzed. RESULTS: 209 eyes suffering stage II to IV IMH were identified. For stage II IMH, the spontaneous closure rate was 8.9%, the initial success rate of intravitreal injections (IVI) of expansile gas and pars plana vitrectomy (PPV) + internal limiting membrane peeling (ILMP) + air tamponade was 84.2% and 100%, respectively. The initial success rate of PPV + ILMP + air tamponade for stage III and stage IV IMH was 89.8% and 86.4%, respectively. Following our intervention strategy, stage II IMH achieved a final IMH closure rate of 100%, stage III of 99% and stage IV of 97%. The final best corrected visual acuity was significantly improved (P < 0.05). Sitting position air-fluid (A-F) exchange alone successfully induced IMH closure in 7/19 eyes that did not achieve IMH closure by initial PPV. For three refractory cases that failed additional PPV + ILM stuffing, intraoperative OCT assisted PPV + sub-retinal BSS injection successfully induced the IMH closure. As the remaining three unclosed IMH cases were dry and stable, no more interventions were conducted. CONCLUSION: The general IMH closure rate based on our therapeutic strategy was satisfactory with a favorable prognosis. IVI expansile gas and sitting position A-F exchange were effective and highly cost-effective under certain circumstances.

4.
J Transl Med ; 19(1): 467, 2021 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-34789263

RESUMO

BACKGROUND: Breast cancer remains one of the most dreadful female malignancies globally, in which cancer stem cells (CSCs) play crucial functions. Circular RNAs have drawn great attention in cancer research area and propofol is a widely applied intravenous anesthetic agent. METHODS: In the current study, we explored the function of circular RNA nucleolar and coiled-body phosphoprotein 1 (circNOLC1) in CSCs of breast cancer and the inhibitory impact of propofol on circNOLC1. RESULTS: The expression of circNOLC1 was induced in breast cancer tissues compared with the non-tumor tissues. The silencing of circNOLC1 was able to repress the viability of breast cancer cells. Meanwhile, the numbers of colony formation were suppressed by circNOLC1 knockdown in breast cancer cells. The inhibition of circNOLC1 reduced the invasion and migration ability of breast cancer cells. The mRNA and protein levels of E-cadherin were enhanced but Vimentin levels were reduced by the silencing of circNOLC1. The repression of circNOLC1 decreased the side population (SP) ratio in breast cancer cells. Meanwhile, the sphere formation ability of breast cancer cells was attenuated by the silencing of circNOLC1. The levels of ATP-binding cassette (ABC) superfamily G member 2 (ABCG2), c-Myc, B cell-specific Moloney murine leukemia virus integration site 1 (Bmi1), and SRY-box transcription factor 2 (Sox2) were repressed by the depletion of circNOLC1 in the cells. Regarding to the mechanism, circNOLC1 functioned as a competing endogenous RNAs (ceRNAs) for microRNA-365a-3p (miR-365a-3p) and the inhibition of miR-365a-3p rescued circNOLC1 depletion-repressed proliferation and cancer stem cell activity of breast cancer. MiR-365a-3p targeted signal transducer and activator of transcription 3 (STAT3) in breast cancer cells and circNOLC1 enhanced STAT3 expression by sponging miR-365a-3p. The overexpression of STAT3 could reverse miR-365a-3p or circNOLC1 depletion-inhibited proliferation and cancer stem cell properties of breast cancer. Interestingly, the expression of circNOLC1 and STAT3 was repressed by the treatment of propofol. The enrichment of STAT3 on circNOLC1 promoter was inhibited by propofol. The expression of circNOLC1 was suppressed by the silencing of STAT3 in the cells. The inhibition of circNOLC1 expression by propofol was rescued under the co-treatment of STAT3 overexpression. The overexpression of circNOLC1 rescued propofol-attenuated proliferation and cancer stem cell functions in vitro and in vivo. CONCLUSIONS: Thus, we concluded that circNOLC1 contributes to CSCs properties and progression of breast cancer by targeting miR-365a-3p /STAT3 axis and propofol inhibited circNOLC1 by repressing STAT3 in a feedback mechanism.

5.
Arthritis Res Ther ; 23(1): 279, 2021 11 04.
Artigo em Inglês | MEDLINE | ID: mdl-34736521

RESUMO

BACKGROUND: Dysregulation of T cells mediated immune responses is a hallmark in the development of systemic lupus erythematosus (SLE). Recent genome wide association study (GWAS) revealed the genetic contribution of variants located in the cytotoxic T lymphocyte-associated protein-4 (CTLA4)-inducible T cell co-stimulator (ICOS) intergenic region to SLE susceptibility. Our aim is to find a functional variant in this region. METHODS: The genetic association results in the CTLA4-ICOS region from previous GWAS were adopted to select the potential variant which was further replicated in two independent cohorts (Henan cohort 2053 SLE patients and 1845 healthy controls, Beijing cohort 2303 SLE patients and 19,262 healthy). In order to explore the functional significance in SLE, bioinformatics with validation experiments (including electrophoretic mobility shift assay and luciferase reporter assay) and mRNA expression analysis were also performed. RESULTS: A variant located in the CTLA4-ICOS intergenic region, rs17268364, was associated with susceptibility to SLE patients in Chinese populations (risk allele, pmeta = 7.02×10-11, OR 1.19, 95%CI 1.13-1.26). The bioinformatics suggested that rs17268364 might affect the expression of CTLA4, not ICOS. The rs17268364 risk G allele containing sequence reduced the expression of the reporter gene by binding transcriptional repressor Ewing sarcoma breakpoint region 1 (EWSR1). Following genotype-mRNA expression, the analysis also showed the risk allele of rs17268364 was associated with low CTLA4 expression in lupus nephritis (LN) patients. Healthy individuals carrying rs17268364 risk G allele was significantly correlated with higher levels of IFN-α signature including increased lymphocyte antigen 6E (LY6E) (p=0.031), interferon-stimulated gene 15 (ISG15) (p=0.038), interferon regulatory factor 9 (IRF9) (p=0.028), and interferon regulatory factor 5 (IRF5) (p=0.040) mRNA expression. CONCLUSIONS: The present study confirmed the functional role of rs17268364 in the CTLA4-ICOS intergenic region that increased SLE susceptibility in the Chinese population.


Assuntos
Estudo de Associação Genômica Ampla , Lúpus Eritematoso Sistêmico , Alelos , Antígeno CTLA-4/genética , Estudos de Casos e Controles , Predisposição Genética para Doença/genética , Genótipo , Humanos , Fatores Reguladores de Interferon , Lúpus Eritematoso Sistêmico/genética , Polimorfismo de Nucleotídeo Único/genética , Proteína EWS de Ligação a RNA
6.
Zhongguo Zhong Yao Za Zhi ; 46(20): 5284-5290, 2021 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-34738431

RESUMO

This study aimed to investigate the enhancing effect of muscone on the transdermal penetration of traditional Chinese medicine ingredients and explore its possible mechanism of action. The Franz diffusion cells were employed to investigate the effect of muscone on the transdermal permeation of a series of model drugs with a wide range of log P values. The solubilities at saturation and the stratum corneum(SC)/vehicle partition coefficients of model drugs were measured to evaluate the effect of muscone on drug thermodynamic activities and partition of drugs into SC. Attenuated total reflectance-Fourier transform infrared spectroscopy(ATR-FTIR) was employed to explore the effect of muscone on the molecular structure of SC. The results showed that muscone significantly promoted the transdermal penetration of hydrophilic and lipophilic drugs, and the enhancement ratio(ER) increased with the decrease in the log P. Muscone could interact with the SC lipids to increase the disorder and fluidity of lipid bilayer packing, which improved skin permeability and promoted transdermal absorption of drugs. This study provides a scientific basis for the application of muscone in traditional Chinese medicine topical preparations.


Assuntos
Medicina Tradicional Chinesa , Absorção Cutânea , Administração Cutânea , Animais , Cicloparafinas , Permeabilidade , Ratos , Ratos Sprague-Dawley , Pele/metabolismo
7.
J Sci Food Agric ; 2021 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-34778959

RESUMO

BACKGROUND: Large yellow croaker (Pseudosciaena crocea) has important commercial value because of its high nutritional value and delicious taste. However, large yellow croaker is readily affected by microorganisms during storage, which causes the corruption of muscle tissue. Both chitosan and apple polyphenols are bio-preservatives, which can effectively inhibit the growth of microorganisms and improve the quality of large yellow croaker. The effects of 10.0 g/L and 20.0 g/L chitosan (CS) combined with 1.0 g/L apple polyphenols (AP) coating on the quality and microbial composition of large yellow croaker during ice storage were investigated respectively. RESULTS: CS+AP coating restrained the increase of TVB-N and biogenic amines, slowed down the rise of K-value and retarded the growth of microorganisms. The bacteriostatic effect was positively correlated with the concentration of CS. Through the analysis of high throughput sequencing (HTS), the microbial diversity was changed respectively. The proportion of Shewanella was significantly decreased by CS+AP coating treatment and Pseudomonas was the dominant microorganism in spoiled samples. Compared with the shelf-life of CK group (8 days), 20.0 g/L CS combined with 1.0 g/L AP coating treatment could extend the shelf-life of large yellow croaker for another 8 days. CONCLUSIONS: CS combined with AP coating may be considered a promising method to delay the biochemical changes of ice stored large yellow croaker and extend its shelf life. This article is protected by copyright. All rights reserved.

8.
Mitochondrial DNA B Resour ; 6(10): 3073-3075, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34595340

RESUMO

The classification of Badidae family based on morphology has been revised several times, but data on complete mitogenome are scarce, the complete mitochondrial genome of the Badidae fish Dario dario was characterized for the first time in the present study. The whole mitogenome was 16,830 bp in size and consisted of 13 protein-coding genes, 22 tRNAs, two rRNAs genes, a control region and origin of light-strand replication. The proportion of coding sequences with a total length of 11,431 bp was 67.92%, which encoded 3800 amino acids. The genome composition was highly A + T biased (58.12%), and exhibited a negative AT-skew (-0.0045) and GC-skew (-0.2347). All protein-coding genes started with ATG except for GTG in CO1, while stopped with the standard TAN codons or a single T. The control region (D-loop) ranging from 15,658 bp to 16,830 bp was 1173 bp in size. Phylogenetic analysis showed that D. dario was most closely related to Badis badis. The complete mitochondrial genome sequence provided new insight into taxonomic classification, and a more complex picture of species diversity within the Anabantiformes.

9.
Appl Opt ; 60(29): 9167-9179, 2021 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-34623999

RESUMO

Quantitative analysis and identification of unknown shaped defects have always been difficult and challenging in the quality control of micro pipes. A series of algorithms for defect detection and feature recognition is presented in this study. A lightweight convolution neural network (LCNN) is introduced to realize defect discrimination. A shallow segmentation network is employed to cooperate with LCNN to obtain pixel-wise crack detection, and a feature recognition algorithm for quantitative measurement is presented. The experimental results show that the proposed algorithms can achieve defect detection with an accuracy of 98.5%, segmentation with mean intersection over union of 0.834, and latency of only 0.2 s. It can be used for online feature recognition and defect detection of the inner surface of a hole.

10.
Materials (Basel) ; 14(20)2021 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-34683592

RESUMO

The construction of superhydrophobic surfaces necessitates the rational design of topographic surface structure and the reduction of surface energy. To date, the reported strategies are usually complex with multi-steps and costly. Thus, the simultaneous achievement of the two indispensable factors is highly desired, yet rather challenging. Herein, we develop a novel structure engineering strategy of realizing the fabrication of a functionally integrated device (FID) with a superhydrophobic surface via a one-step spraying method. Specifically, silica nanoparticles are used to control the surface roughness of the device, while polydimethylsiloxane is employed as the hydrophobic coating. Benefitting from the adopted superhydrophobicity, the as-fabricated FID exhibits a continuous, excellent oil-water separating performance (e.g., 92.5% separating efficiency) when coupled with a peristaltic pump. Notably, a smart design of incorporating a gas switch is adopted in this device, thereby effectively preventing water from entering the FID, realizing thorough oil collection, and avoiding secondary pollution. This work opens up an avenue for the design and development of the FID, accessible for rapid preparation and large-scale practical application.

11.
Molecules ; 26(20)2021 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-34684689

RESUMO

There is an ever-increasing trend toward bendable and high-energy-density electrochemical storage devices with high strength to fulfil the rapid development of flexible electronics, but they remain a great challenge to be realised by the traditional slurry-casting fabrication processes. To overcome these issues, herein, a facile strategy was proposed to design integrating an electrode with flexible, high capacity, and high tensile strength nanosheets with interconnected copper micro-fibre as a collector, loaded with a novel hierarchical SnO2 nanoarchitecture, which were assembled into core-shell architecture, with a 1D micro-fibre core and 2D nanosheets shell. When applied as anode materials for LIBs, the resultant novel electrode delivers a large reversible specific capacity of 637.2 mAh g-1 at a high rate of 1C. Such superior capacity may benefit from rational design based on structural engineering to boost synergistic effects of the integrated electrode. The outer shell with the ultrathin 2D nanoarchitecture blocks can provide favourable Li+ lateral intercalation lengths and more beneficial transport routes for electrolyte ions, with sufficient void space among the nanosheets to buffer the volume expansion. Furthermore, the interconnected 1D micro-fibre core with outstanding metallic conductivity can offer an efficient electron transport pathway along axial orientation to shorten electron transport. More importantly, the metal's remarkable flexibility and high tensile strength provide the hybrid integrated electrode with strong bending and stretchability relative to sintered carbon or graphene hosts. The presented strategy demonstrates that this rational nanoarchitecture design based on integrated engineering is an effective route to maintain the structural stability of electrodes in flexible LIBs.

12.
Neuron ; 2021 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-34678146

RESUMO

To successfully perform goal-directed navigation, animals must know where they are and what they are doing-e.g., looking for water, bringing food back to the nest, or escaping from a predator. Hippocampal neurons code for these critical variables conjunctively, but little is known about how this "where/what" code is formed or flexibly routed to other brain regions. To address these questions, we performed intracellular whole-cell recordings in mouse CA1 during a cued, two-choice virtual navigation task. We demonstrate that plateau potentials in CA1 pyramidal neurons rapidly strengthen synaptic inputs carrying conjunctive information about position and choice. Plasticity-induced response fields were modulated by cues only in animals previously trained to collect rewards based on available cues. Thus, we reveal that gradual learning is required for the formation of a conjunctive population code, upstream of CA1, while plateau-potential-induced synaptic plasticity in CA1 enables flexible routing of the code to downstream brain regions.

13.
Materials (Basel) ; 14(19)2021 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-34639896

RESUMO

The development of fifth-generation (5G) communication and wearable electronics generates higher requirements for the mechanical properties of copper foil. Higher mechanical properties and lower resistance are required for flexible copper-clad laminate and high-frequency and high-speed Cu foil. Deep cryogenic treatment (DCT), as a post-treatment method, has many advantages, such as low cost and ease of operation. However, less attention has been paid to the impact of DCT on rolled Cu foil. In this study, the effects of DCT on the microstructure and mechanical properties of rolled Cu foil were investigated. The results show that as the treatment time increased, the tensile strength and hardness first increased and then decreased, reaching a peak value of 394.06 MPa and 1.47 GPa at 12 h. The mechanical property improvement of rolled Cu foil was due to the grain refinement and the increase of dislocation density. The dislocation density of rolled Cu foil after a DCT time of 12 h was determined to have a peak value of 4.3798 × 1015 m-2. The dislocation density increased by 19% and the grain size decreased by 12% after 12 h DCT.

14.
Ultrason Sonochem ; 79: 105787, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34634550

RESUMO

The effects of multi-frequency ultrasound on surface decontamination and structural characteristics of large yellow croaker (Pseudosciaena crocea) during refrigerated storage were evaluated. The results of total viable counts (TVCs) and psychrophilic bacteria counts (PBCs) demonstrated that multi-frequency ultrasound retarded the growth of microorganisms. The bacteriostatic effect was positively correlated with the increase of ultrasound frequencies. However, compared with triple-frequency ultrasound (TUS, 20/28/40 kHz) treatment, dual-frequency ultrasound (DUS, 20/28 kHz) treatment had higher water-holding capacity (WHC) and immobilized water content, better texture characteristics, lower pH and total volatile basic nitrogen (TVB-N). Through the results of myofibrillar fragmentation index (MFI), intrinsic fluorescence intensity (IFI) and atomic force microscope (AFM), multi-frequency ultrasound could effectively stabilize the myofibrillar protein structure of refrigerated large yellow croaker, which could maintain better texture characteristics. The effects of DUS were the most significant. Therefore, multi-frequency ultrasound treatment could inhibit the growth of microorganisms and improve the structural characteristics of large yellow croaker during refrigerated storage.

15.
ACS Appl Mater Interfaces ; 13(43): 51661-51672, 2021 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-34696581

RESUMO

Supramolecular host-guest chemistry bridging the adjustable amphiphilicity and macromolecular self-assembly is well advanced in aqueous media. However, the interfacial self-assembled behaviors have not been further exploited. Herein, we designed a ß-cyclodextrin-grafted alginate/azobenzene-functionalized dodecyl (Alg-ß-CD/AzoC12) supra-amphiphilic system that possessed tunable amphiphilicity by host-guest interfacial self-assembly. Especially, supra-amphiphilic aggregates could be utilized as highly efficient soft colloidal emulsifiers for stabilizing water-in-oil-water (W/O/W) Pickering emulsions due to the excellent interfacial activity. Meanwhile, the assembled particle structures could be modulated by adjusting the oil-water ratio, resulting from the tunable aggregation behavior of supra-amphiphilic macromolecules. Additionally, the interfacial adsorption films could be partially destroyed/reconstructed upon ultraviolet/visible irradiation due to the stimuli-altering balance of amphiphilicity of Alg-ß-CD/AzoC12 polymers, further constructing the stimulus-responsive Pickering emulsions. Therefore, the supramolecular interfacial self-assembly-mediated approach not only technologically advances the continued development of creative templates to construct multifunctional soft materials with anisotropic structures but also serves as a creative bridge between supramolecular host-guest chemistry, colloidal interface science, and soft material technology.

16.
Cancer ; 2021 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-34623636

RESUMO

BACKGROUND: The objective of this study was to assess whether entecavir (ETV) in combination with interferon-α (IFN-α) could reduce hepatocellular cancer (HCC) and extrahepatic cancers (EHCs) in patients with chronic hepatitis B (CHB). METHODS: The cohort consisted of 4194 patients with CHB treated with ETV combined with IFN-α or ETV monotherapy at a tertiary hospital in Beijing, China, from January 2009 to December 2017. The risks, hazard ratios (HRs), and 95% confidence intervals (CIs) of HCC and EHCs were compared in the 2 groups. RESULTS: In a multivariate Cox regression analysis, a significantly lower risk of HCC (HR, 0.6; 95% CI, 0.3-0.9; P = .0310) and a marginally significantly lower risk of EHCs (HR, 0.2; 95% CI, 0.02-1.3; P = .0854) were observed in the group receiving ETV combined with IFN-α in comparison with the ETV monotherapy group. The annual virological response rates were significantly higher in the combination therapy group versus the monotherapy group (33.8% vs 21.2%; P < .0001), but the hepatitis B surface antigen (HBsAg) seroclearance rates were not (1.2% vs 0.9%; P = .8537). The HRs were consistent with propensity score-based matching, inverse probability weighting adjustments, and adjustments for virological response and HBsAg seroclearance. CONCLUSIONS: ETV combined with IFN-α therapy is superior to ETV monotherapy in reducing the risk of HCC and EHCs for patients with CHB. People who can tolerate and benefit from IFN-α therapy could consider combination therapy.

17.
Sci Total Environ ; 806(Pt 3): 150736, 2021 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-34600985

RESUMO

Humic-reducing microorganisms (HRMs) can utilize humic substance as terminal electron mediator promoting the bioremediation of contaminate, which is ubiquitous in composts. However, the impacts of HRMs on antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) in composts and different HRMs community composition following the types of biowastes effected the spread of ARGs have not been investigated. Herein, the dynamics and mobility of ARGs and HRMs during protein-, lignocellulose- and lignin-rich composting were investigated. Result show that ARGs change significantly at the thermophilic phase, and the relative abundance of most ARGs increase during composting. Seven groups of HRMs communities are classified as primary host HRMs of ARGs, and most host HRMs groups from protein-rich composts. Conclusively, regulating methods for inhibiting ARGs spread for different composts are proposed. HRMs show a higher ARGs dissemination capacity in protein-rich composts than lignocellulose- and lignin-rich composts, but the spread of ARGs can be inhibited by regulate physicochemical parameters in protein-rich composts. In contrary, most HRMs have inhibitory effects on ARGs spread in lignocellulose- and lignin-rich composts, and those HRMs can be used as a new agent that inhibits the spread of ARGs. Our results can help in understanding the potential risk spread of ARGs by inoculating functional bacteria derived from different biowastes composts for environmental remediation, given their expected importance to developing a classification-oriented approach for composting different biowastes.

18.
Sci Total Environ ; 807(Pt 1): 150759, 2021 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-34619190

RESUMO

Ocean acidification in coastal seawaters is a complex process, with coastal pH being affected by numerous factors including watershed and biological processes that also support metabolically diverse bacterial communities. The world's largest macroalgal blooms have occurred consecutively in the Yellow Sea over the last 13 years. In particular, algal mats formed by Yellow Sea green tides (YSGT) significantly influence coastal environments. Herein, we hypothesized that 1) inorganic carbonate chemistry in coastal areas is altered by diel metabolism of these giant algal mats and that 2) bacterial community composition in diffusive boundary layers might be altered along diel cycles due to algal mat metabolism. In situ studies indicated that algal mat metabolism led to changes in diel pH and CO2 in affected seawaters. Such metabolic activities could intensify diel pH fluctuations in algal mat diffusive boundary layers, as noted by pH fluctuations of 0.22 ± 0.01 units, and pCO2 fluctuations of 214.62 ± 29.37 µatm per day. In contrast, pH fluctuations of 0.11 ± 0.02 units and pCO2 fluctuations of 79.02 ± 42.70 µatm were noted in unaffected areas. Furthermore, the bacterial community composition associated with diffusive algal boundary layers, including those of ambient bacteria and epiphytic bacteria, exhibited diel changes, while endophytic bacterial communities were relatively stable. Flavobacteriaceae were particularly highly abundant taxa in the ambient and epiphytic bacterial communities and exhibited increased abundances at night but sharp decreases in abundances during daytime. Flavobacteriaceae are heterotrophic taxa that could contribute to coastal area acidification at night due to the transformation of organic carbon to inorganic carbon. These results provide new insights to understand the variability in coastal ocean acidification via harmful algal blooms while providing a framework for evaluating the effects of YSGT on costal carbon cycling.

19.
Carbohydr Polym ; 273: 118492, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34560936

RESUMO

The separation of crude oil from oily water and collection of the emulsion constituents has attracted significant attention. We demonstrate that the relationships between inherent dynamic factors and the performance of a Pickering emulsion stabilized by SiO2 particles with adsorbed hydrophobically modified sodium alginate derivatives (HMSA), a natural pH-sensitive polysaccharide, can be clarified via a multi-scale methodology. Functionalization of the silica surface with HMSA controls particle dispersibility, as verified by turbidity and stability analyses, the zeta potential, and transmission electron microscopy measurements. The interaction mechanism between HMSA and SiO2 nanoparticles was elucidated by both experimental adsorption measurements and computer simulations, which showed qualitative consistency. The aggregation/disaggregation of HMSA/SiO2 particles achieved by tuning the pH of the solution facilitated reversible dispersibility/collectability behavior. Overall, a high-viscosity Pickering emulsion system based on particle-particle and droplet-droplet interactions, which can be filtered for the recovery of spilled crude oil, was demonstrated.

20.
Int J Biol Macromol ; 190: 11-18, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34478791

RESUMO

The development of advanced energy storage systems, such as rechargeable batteries and supercapacitors (SCs), is one of the great challenges related to energy demand with the rapid development of world economy. Herein, a three-dimensional hierarchical porous lignin-derived carbon/WO3 (HPC/WO3) was prepared by carbonization and solvothermal process. This electrode material for supercapacitor can be operated at a wide voltage window range of -0.4 V to 1.0 V. More importantly, 3HPC/WO3 with ultrahigh mass loading (~3.56 mg cm-2) has excellent specific capacitance of 432 F g-1 at 0.5 A g-1 and cycling stability of 86.6% after 10,000 cycles at 10 A g-1. The as-assembled asymmetrical supercapacitor shows an energy density of 34.2 W h kg-1 at a power density of 237 W kg-1 and energy density of 16 W h kg-1 at a power density is 14,300 W kg-1. A solid-state planar micro-supercapacitor (MSC) was fabricated using HPC/WO3 nanocomposites. Moreover, the calculated specific capacity of MSC was 20 mF cm-2 in polyvinyl alcohol-sulfuric acid gel electrolyte. Overall, through the reasonable design of HPC/WO3 nanocomposite materials and the efficient assembly of MSCs, the performance of the device was greatly improved, thus providing a clear strategy for the development of energy storage devices.

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