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1.
Air Qual Atmos Health ; : 1-14, 2021 Apr 02.
Artigo em Inglês | MEDLINE | ID: mdl-33824688

RESUMO

Emergency response mechanisms were activated throughout China during the COVID-19 outbreak. It is different from the temporary, partial, and limited pollution control measures taken to ensure the regional environmental quality during several important events such as the 2008 Beijing Olympic Games and the 2014 Asia-Pacific Economic Cooperation (APEC). During the COVID-19 epidemic period, extensive movement of people and almost all unnecessary industrial production (necessary industrial production refers to the production of food, epidemic prevention materials, etc.) have been severely restricted, so transportation and industrial production have been greatly reduced. This is a rare extreme emission reduction scenario that presents a unique opportunity for atmospheric research. In this study, based on hourly mass concentration data of NO2 and SO2 from atmospheric monitoring sites in the Beijing-Tianjin-Hebei (BTH) region during the COVID-19 epidemic period, the changes in transportation and industrial production in the region, data statistics, and spatial analysis were used to analyze the pollution changes and their causes. The results indicate that the NO2 and SO2 concentrations in the BTH region decreased significantly during the epidemic period. The spatial distribution pattern of NO2 pollution in the BTH region was "high in the southeast and low in the northwest," and SO2 pollution in the BTH region was high in the southern and eastern parts of Hebei. The initiation of emergency response level 1 had an obvious effect on reducing NO2 and SO2 pollution in the region, while the impact of emergency response level 2 and below was limited. Compared with the single traffic control, the comprehensive control, similar to the emergency response, had a better effect on reducing NO2 pollution in the region. The control of major large cities in the region also had a certain effect on alleviating NO2 and SO2 pollution in the entire region. Moreover, for activities under short-term control, it is particularly important to guard against the "retaliatory growth" after the control is lifted. By reducing and controlling some polluting industries in industrial production, the degree of NO2 and SO2 pollution in the region can be effectively reduced. The manufacturing industry of chemical raw materials and the chemical products and non-metallic mineral products industry made a great contribution to the change in industrial source pollution emissions in the BTH region during the COVID-19 epidemic. Road traffic emissions remained an important source of NO2 emissions in the BTH region during this period. NO2 emission reduction can be effectively achieved by controlling road traffic and transportation.

2.
Heart Vessels ; 2021 Apr 08.
Artigo em Inglês | MEDLINE | ID: mdl-33830315

RESUMO

The mutation MYBPC3-E334K is a culprit mutation of hypertrophic cardiomyopathy (HCM). The pathogenicity of MYBPC3-E334K is conflicting in ClinVar because of the limited segregation data and the relatively high frequency in gnomAD (0.03% overall, with 0.3% in East Asians and 0.8% in Japanese). The main aim is to clarify the clinical importance and phenotype-genotype correlations in subjects with or without MYBPC3-E334K alone. The prevalence of MYBPC3-E334K was sequenced in 1017 HCM unrelated probands. The clinical features, morphology phenotypes, and electrical phenotypes were further analyzed according to the phenotype and genotype status in families with single-mutation MYBPC3-E334K. Nine of 1017 (0.88%) unrelated HCM probands were detected harboring MYBPC3-E334K, and three of them harbored a second variant in sarcomere protein gene. Family study and co-segregation analyses indicated that patients with single-mutation MYBPC3-E334K showed autosomal dominant mode of inheritance with incomplete penetrance. The overall disease penetrance was 52.6%, and the disease penetrance was higher in males than in females (100% in men vs 25% in women, p = 0.003). The mean age at diagnosis of males was approximately 25 years younger than females (36.57 ± 18.65 vs 62.33 ± 12.10, p = 0.062). The variant MYBPC3-E334K was classified as a likely pathogenic variant, and a second sarcomere variant did not reveal obvious cumulative effects. The patients harboring single-mutation MYBPC3-E334K had incomplete penetrance, and males demonstrated higher penetrance and early onset HCM than females. A second sarcomere variant did not reveal obvious cumulative effects.

3.
Plant Cell Environ ; 2021 Apr 18.
Artigo em Inglês | MEDLINE | ID: mdl-33866595

RESUMO

Soybean mosaic virus (SMV) is one of the most widespread and devastating viral diseases worldwide. The genetic architecture of qualitative resistance to SMV in soybean remains unclear. Here, the Rsvg2 locus was identified as underlying soybean resistance to SMV by genome-wide association and linkage analyses. Fine mapping results showed that soybean resistance to SMV strains G2 and G3 was controlled by a single dominant gene, GmST1, on chromosome 13, encoding a sulfotransferase (SOT). A key variation at position 506 in the coding region of GmST1 associated with the structure of the encoded SOT and changed SOT activity levels between RSVG2-S and RSVG2-R alleles. In RSVG2-S allele carrier 'Hefeng25', the overexpression of GmST1 carrying the RSVG2-R allele from the SMV-resistant line 'Dongnong93-046' conferred resistance to SMV strains G2 and G3. Compared to Hefeng25, the accumulation of SMV was decreased in transgenic plants carrying the RSVG2-R allele. SMV infection differentiated both the accumulation of jasmonates and expression patterns of genes involved in jasmonic acid (JA) signaling, biosynthesis, and catabolism in RSVG2-R and RSVG2-S allele carriers. This characterization of GmST1 suggests a new scenario explaining soybean resistance to SMV. This article is protected by copyright. All rights reserved.

4.
Nanoscale ; 2021 Apr 26.
Artigo em Inglês | MEDLINE | ID: mdl-33899868

RESUMO

Five-coordinate geometry around ruthenium with highly exposed active sites has attracted intensive scientific interest due to its superior properties and extensive applications. Herein, we report a series of structurally controllable multi-Ru-bridged polyoxometalates, K5NaH10[{Ru4(H2O)n}(WO2)4(AsW9O33)4]·mH2O {1, 1-dehyd-373K, 1-dehyd-473K, 1-dehyd-573K; n = 4, m = 36; n = 4, m = 6; n = 4, m = 0; n = 0, m = 0} fabricated through a feasible assembly strategy using arsenotungstate {2, KNa12H17Cl2(As4W40O140)·29H2O} as a structure-directing unit. Systematic characterization methods identified that the six-coordinate geometry can successfully transform into five-coordinate geometry about active sites (Ru) by removing aqua ligands under high reaction temperatures. All the multi-Ru-bridged polyoxometalates demonstrated strong stability and catalytic effectiveness in the transformation of 1-(4-chlorophenyl)ethanol to 4'-chloroacetophenone under very mild conditions. 1-dehyd-573K, specifically, achieves the best catalytic effectiveness with a turnover frequency (TOF) = 25 100·h-1 owing to its unique five-coordinate geometry on the Ru sites. To our knowledge, 1-dehyd-573K outperforms other POM-based catalysts in the oxidative catalysis of 1-(4-chlorophenyl)ethanol. The heterogeneous polyoxometalates were also proven to be strongly reusable, with their structural integrities well maintained after multiple-cycle catalytic reactions.

5.
J Int Adv Otol ; 17(2): 182-185, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33893790

RESUMO

Traumatic perilymphatic fistula (PLF) is an uncommon cause of acute vestibular symptoms and hearing loss following head injury in children. We describe the management of 3 pediatric patients with traumatic PLF using an endoscopic ear surgery (EES) approach. Three pediatric patients with traumatic PLF underwent repair via an EES approach between August and October 2018. Patients included a 14-year-old female (oval window), a 13-year-old male (round window), and a 10-month-old male (oval and round window). Ossicular chain injury was identified and repaired in 2 patients. The 10-month-old patient required a second-stage surgery that included lumbar drain placement and a post-auricular, endoscopic-assisted approach due to an especially brisk leak. All patients had complete resolution of vestibular symptoms post-operatively with no recurrence at a mean follow-up of 8.3 months. Traumatic PLF can be safely and effectively diagnosed and managed via an EES approach in children, though an endoscopic-assisted approach may be necessary in select cases due to factors such as patient age and leak severity.

6.
Chemosphere ; 279: 130531, 2021 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-33887591

RESUMO

The research on geochemical behaviors of iodine is significant for deep understanding of the source and distribution of iodine on the earth. However, as one of the most important sources, the ocean emissions and relative transport pathways of iodine, as well as the preservation after deposition are still not well known, especially in the arid areas of central Asia. A peat sediment core collected nearby Barkol Lake from northwest China was analyzed for iodine isotopes (127I and 129I). The observed high 127I concentration in the top 2 cm indicated a significant accumulation of iodine in the surface oxic conditions due to the continuous sources of incompletely decomposed organic matter. Dissociation of iodine into pore waters occurred once the anoxic conditions established beneath the surface by a serial reduction reaction during the degradation of organic matter. The temporal variation of anthropogenic 129I in the peat sediment recorded its sources and transport pathways. Besides the global fallout 129I during late 1950s and early 1960s, the significantly increased air releases from the European nuclear fuel reprocessing plants during 1975-1997 and the increased marine discharges since 1990s contributed the major portion of 129I in the peat core. The major transport pathway of 129I from the Europe was through Westerlies following the re-emission of the marine discharged 129I to the atmosphere, indicating a clearly ocean emitted iodine in the concerned central Asia.

8.
Front Immunol ; 12: 648182, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33841431

RESUMO

Proteins in the signaling lymphocytic activating molecule (SLAM) family play crucial roles in regulating the immune system. CD244 (SLAMF4) is a protein in this family, and is also a member of the CD2 subset of the immunoglobulin (Ig) superfamily. CD244 is a cell surface protein expressed by NK cells, T cells, monocytes, eosinophils, myeloid-derived suppressor cells, and dendritic cells. CD244 binds to the ligand CD48 on adjacent cells and transmits stimulatory or inhibitory signals that regulate immune function. In-depth studies reported that CD244 functions in many immune-related diseases, such as autoimmune diseases, infectious diseases, and cancers, and its action is essential for the onset and progression of these diseases. The discovery of these essential roles of CD244 suggests it has potential as a prognostic indicator or therapeutic target. This review describes the molecular structure and function of CD244 and its roles in various immune cells and immune-related diseases.

10.
Genomics ; 113(3): 1262-1271, 2021 Mar 06.
Artigo em Inglês | MEDLINE | ID: mdl-33689785

RESUMO

Sclerotinia stem rot (SSR) is a disease of soybean [Glycine max (L.) Merr] that causes severe yield losses. We studied 185 representative soybean accessions to evaluate partial SSR resistance and sequenced these by the specific-locus amplified fragment sequencing method. In total, 22,048 single-nucleotide polymorphisms (SNPs), with minor allele frequencies (MAF) ≥5% and missing data <3%, were developed and applied to genome-wide association study of SSR responsiveness and assess linkage disequilibrium (LD) level for candidate gene selection. We identified 18 association signals related to SSR partial resistance. Among them, six overlapped the regions of previous quantitative trait loci, and twelve were novel. We identified 243 candidate genes located in the 200 kb genomic region of these peak SNPs. Based on quantitative real-time polymerase chain reaction and haplotype analysis, Glyma.03G196000 and Glyma.20G095100, encoding pentatricopeptide repeat proteins, might be important factors in the resistance response of soybean to SSR.

11.
Molecules ; 26(4)2021 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-33672038

RESUMO

Our previous study found that desmethylxanthohumol (1) inhibited α-glucosidase in vitro. Recently, further investigations revealed that dehydrocyclodesmethylxanthohumol (2) and its dimer analogue rottlerone (3) exhibited more potent α-glucosidase inhibitory activity than 1. The aim of this study was to synthesize a series of rottlerone analogues and evaluate their α-glucosidase and DPP-4 dual inhibitory activity. The results showed that compounds 4d and 5d irreversibly and potently inhibited α-glucosidase (IC50 = 0.22 and 0.12 µM) and moderately inhibited DPP-4 (IC50 = 23.59 and 26.19 µM), respectively. In addition, compounds 4d and 5d significantly promoted glucose consumption, with the activity of 5d at 0.2 µM being comparable to that of metformin at a concentration of 1 mM.


Assuntos
Inibidores da Dipeptidil Peptidase IV/farmacologia , Flavonoides/síntese química , Flavonoides/farmacologia , Glucose/metabolismo , Inibidores de Glicosídeo Hidrolases/farmacologia , Propiofenonas/síntese química , Propiofenonas/farmacologia , Dipeptidil Peptidase 4/metabolismo , Flavonoides/química , Células Hep G2 , Humanos , Cinética , Propiofenonas/química , alfa-Glucosidases/metabolismo
12.
Huan Jing Ke Xue ; 42(3): 1205-1214, 2021 Mar 08.
Artigo em Chinês | MEDLINE | ID: mdl-33742918

RESUMO

A series of strict control measures were imposed in the Beijing-Tianjin-Hebei region in early 2020 to control the spread of COVID-19. These measures have led to a reduction of anthropogenic air pollutants, providing an opportunity to observe the contribution of human activities to local air pollution. In this study, the control period was divided into four stages:the before, early, middle, and later stages. Based on a variety of data including meteorological, traffic, and industrial manufacturing datasets, statistical methods were combined with spatial analysis to evaluate changes in air pollution and associated human impacts during each stage. In addition, suggestions are made for further regional air pollution control in the Beijing-Tianjin-Hebei area. Key results are as follows:① Overall, the AQI and the concentrations of six air pollutants, especially SO2, PM10, and NO2, were lower during control period than during the equivalent period in 2019 (reductions of 26.5%, 24.3%, and 16.9%, respectively). From the before to later stages, pollutants (except O3) showed a downward trend while O3 increased significantly during the before stage (by 76.2%) and the growth rate slowed during the middle and later stages; ②During the prior stage, Beijing experienced two periods with heavy air pollution days as a result of the local accumulation of pollutants, secondary transformation, and regional transport. The concentration of PM2.5 in February was nearly 60% lower than in February 2014 under similar meteorological conditions in Beijing; ③ Following an increase in traffic volume and industrial activity, changes in air pollutants tended to be stable or slightly increase during the middle and later stages of the control period. The grey relation coefficients between thermal radiation intensity anomalies and the main pollutants in heavy industrial cities were greater than 0.6, which means that the control of industrial emissions remains key to controlling air pollution.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , Poluentes Atmosféricos/efeitos adversos , Poluentes Atmosféricos/análise , Poluição do Ar/análise , Pequim , China , Cidades , Monitoramento Ambiental , Humanos , Pandemias , Material Particulado/análise
13.
Huan Jing Ke Xue ; 42(3): 1245-1254, 2021 Mar 08.
Artigo em Chinês | MEDLINE | ID: mdl-33742922

RESUMO

To study the pollution characteristics, sources, and ecological and health risk of PM2.5-bound metallic elements in road dust in Zibo City, a total of 97 dust samples were collected in eight districts between October 2016 and May 2017, and particles smaller than 2.5 µm were suspended filtered using a resuspension system. Inductively coupled plasma mass spectrometry (ICP-MS) and optical emission spectrometry (ICP-OES) were used to investigate 18 metal elements within the dust samples. The results showed that the mass fraction of Ca[ω(Ca)] was highest with an average of 120307.7 mg·kg-1, which was 7.2 times higher than the soil background values for Shandong Province. The mean values of ω(Zn), ω(Cu), ω(Sb), and ω(Cd) were 13.9, 11.7, 13.3, and 29.6 times higher than the background values, respectively. The geo-accumulation index (Igeo) indicated high levels of Cd, Zn, Cu, and Sb pollution, especially in winter. Enrichment factors (EFs) also indicated high concentrations of Cd, Zn, Sb, and Cu in the road dust, which were notably affected by human activities. Principal component analysis (PCA) showed that biomass combustion, coal burning, vehicle emissions, iron and steel smelting, and soil dust are the five main sources of metal elements in road dust in Zibo City. The potential ecological risk of Cd and the total potential risk were extremely high during three seasons and was highest in winter. Health risk assessment showed that As and Pb had a non-carcinogenic risk for children, while Cr presents a carcinogenic risk. In conclusion, pollution from PM2.5-bound metallic elements in road dust in Zibo City is derived from anthropogenic sources and is most severe during winter. Importantly, the levels of pollution detected represent potential ecological risk as well as some non-carcinogenic and carcinogenic risks for children. Therefore, the source control of road dust requires particular attention.


Assuntos
Metais Pesados , Criança , Cidades , Poeira/análise , Monitoramento Ambiental , Humanos , Metais Pesados/análise , Medição de Risco
14.
ACS Infect Dis ; 2021 Mar 09.
Artigo em Inglês | MEDLINE | ID: mdl-33686850

RESUMO

Integrase strand transfer inhibitors (INSTIs) block the integration step of the retroviral lifecycle and are first-line drugs used for the treatment of HIV-1/AIDS. INSTIs have a polycyclic core with heteroatom triads, chelate the metal ions at the active site, and have a halobenzyl group that interacts with viral DNA attached to the core by a flexible linker. The most broadly effective INSTIs inhibit both wild-type (WT) integrase (IN) and a variety of well-known mutants. However, because there are mutations that reduce the potency of all of the available INSTIs, new and better compounds are needed. Models based on recent structures of HIV-1 and red-capped mangabey SIV INs suggest modifications in the INSTI structures that could enhance interactions with the 3'-terminal adenosine of the viral DNA, which could improve performance against INSTI resistant mutants. We designed and tested a series of INSTIs having modifications to their naphthyridine scaffold. One of the new compounds retained good potency against an expanded panel of HIV-1 IN mutants that we tested. Our results suggest the possibility of designing inhibitors that combine the best features of the existing compounds, which could provide additional efficacy against known HIV-1 IN mutants.

15.
Front Endocrinol (Lausanne) ; 12: 608232, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33737909

RESUMO

Background: The incidence of pediatric type 1 diabetes (T1D) is increasing worldwide, and the appropriate choice of therapy regimens is important for children, especially in developing countries with inadequate resources. Methods: We conducted a design combining meta-analysis and prospective cohort study. In meta-analysis, 14 studies involving 69,085 TID cases reported glycosylated hemoglobin (HbA1c) levels, including 48,363 multiple daily insulin injections therapy (MIT) and 20,722 continuous subcutaneous insulin infusion (CSII). In our prospective cohort study, TID cases were recruited from a tertiary children's hospital, and randomly divided into Group MIT and Group CSII. After the 4-year follow-up, the effects of MDI (n = 112) and CSII (n = 76) therapy on glycemic control, long-term complications, as well as the growth and pubertal development were explored. Results: Compared to CSII in TID, HbA1c levels in MDI (WMD = 0.21, 95% CI: 0.20 to 0.23) were increased significantly in meta-analysis. Among 188 clinical cases, mean age at recruitment was 7.55 (SD 2.91) years. Duration of TID was 4.23 (SD 2.61) years. 50.53% (n = 95) of them were boys. The 4-year follow-up showed that children's HbA1c was 0.67 (95% CI -1.28, -0.05) % lower in children with CSII compared to children with MDI in multivariable regression models with adjustment for potential confounders (children's age at follow-up, duration of TID, gender, birthweight, parity, and delivery method). CSII was associated with 2.31 kg higher in children's weight (95% CI 0.59, 4.04) in the adjusted model. No difference was found in peripheral nerve and fundus consequences as well as the status of obesity and thin and pubertal development between CSII and MIT. Conclusion: CSII might be associated with better glycemic control and better effect for children growth development. No higher risks of long-term complications and delayed pubertal development were observed in CSII. Our findings provided evidence for a better therapy regimen for T1D in children, nevertheless, they need to be validated by a larger sample size study.

16.
Gene ; 784: 145594, 2021 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-33766704

RESUMO

Analysing the molecular regulation mechanism of fat deposition in yellow cattle can provide a theoretical basis for the breeding of excellent beef cattle. ANGPTL8 (angiopoietin-like protein 8) promotes the formation of lipid droplets during adipocyte differentiation. To explore the promoter active region of ANGPTL8 and predict potential transcription factors, we further provide a theoretical basis for the functional analysis and regulatory mechanism of ANGPTL8 in adipogenesis. The promoter region of bovine ANGPTL8 was cloned by overlap extension PCR. Online software was used to predict potential transcription factor binding sites, and it identified PPARγ, SREBP1, C/EBPα, and Znf423 transcription factor binding sites in ANGPTL8 promoter region. A luciferase reporter gene vector which contained different deletion fragments of the ANGPTL8 promoter was constructed. Then, the vectors were cotransfected into 293 T cells with the internal control plasmid pRL-TK by cationic liposomes, and the relative fluorescence intensity was detected by a microplate reader. The results of the luciferase activity analysis showed that the core promoter area of ANGPTL8 was in the -885/-227 bp region of the 5' flanking sequence, while just two SREBP1 binding sites occurred in this area. When SREBP1 was knocked down by siRNA, the expression level of ANGPTL8 was reduced, and we speculated that SREBP1 may be an important transcription factor regulating ANGPTL8 transcription.


Assuntos
Proteínas Semelhantes a Angiopoietina/química , Proteínas Semelhantes a Angiopoietina/genética , Biologia Computacional/métodos , Proteína de Ligação a Elemento Regulador de Esterol 1/genética , Proteínas Semelhantes a Angiopoietina/metabolismo , Animais , Sítios de Ligação , Cruzamento , Bovinos , Clonagem Molecular , Regulação da Expressão Gênica , Células HEK293 , Humanos , Regiões Promotoras Genéticas , Software , Fatores de Transcrição/genética
17.
Chem Biodivers ; 2021 Mar 12.
Artigo em Inglês | MEDLINE | ID: mdl-33709565

RESUMO

Five new peptaibols, longibramides A-E (1-5) with 11 amino acid residues, were isolated from a fungus Trichoderma longibrachiatum Rifai DMG-3-1-1, which was isolated from a mushroom Clitocybe nebularis (Batsch) P. Kumm collected from coniferous forest in the subboreal area of northeast China. The structures of longibramides A-E were determined by their spectroscopic data (NMR and MS-MS spectra), their absolute configurations were determined by X-ray diffractions and Marfey's analyses. The X-ray diffractions of longibramides A, B, and the similar CD spectra of A-E showed that they all had α-helix conformations. Longibramides B and E showed moderate cytotoxicities against BV2 and MCF-7 cells and also showed some inhibitory effects against methicillin-resistant Staphylococcus aureus MRSA T144. L-trans-Hyp was not commonly found in natural peptaibols, which was the 6th or 10th amino acid residue in longibramides C-E. The X-ray diffractions of longibramides A and B afforded the accuracy conformations of their secondary structures, which maybe help to interpret the structure-activity relationships of the family of peptaibols in the future.

18.
J Med Chem ; 64(7): 4196-4205, 2021 Apr 08.
Artigo em Inglês | MEDLINE | ID: mdl-33784102

RESUMO

Natural products have failed to meet the urgent need for drug discovery in recent decades due to limited resources, necessitating new strategies for re-establishing the key role of natural products in hit screening. This work introduced DNA-encoding techniques into the synthesis of phenolic acid-focused libraries containing 32 000 diverse compounds. Online selection of the library using immobilized angiotensin II type I receptor (AT1R) resulted in seven phenolic acid derivatives. The half-maximal concentration (IC50) of hit 1 for the right shift of the [125I]-Sar1-AngII competition curve was 19.6 nM. Pharmacological examination of renovascular hypertensive rats demonstrated that hit 1 significantly lowered the blood pressure of the animals without changing their heart rates. These results were used to create a general strategy for rapid and unbiased discovery of hits derived from natural products with high throughput and efficiency.

19.
Food Chem ; 354: 129581, 2021 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-33756319

RESUMO

The effect of fish gelatin (FG) and grape seed extract (GSE) on microbiota composition and moisture state of fish was unexplored. Herein, this study aimed to evaluate the single and combined (FGG) effects on seabass during storage (4 °C) with assistant of vacuum impregnation and to elucidate the underlying preservative mechanism. As suggested by low-field NMR and magnetic resonance imaging, FGG-treated seabass presented higher water holding capacity by controlling transformation from immobilised to free water. Moreover, the total viable count and spoilage bacteria were reduced by > 1 log CFU/g as compared to the control. Changes in microbial flora analysed using high throughput sequencing further indicated that GSE contributed to the notably suppressed growth of Pseudomonas. Also, the accumulation of biogenic amines especially putrescine was decreased (over 0.5-fold) under the combination treatment as compared to the control (P < 0.05). The results suggest that FGG is promising for seabass preservation.

20.
Acupunct Med ; : 964528421997435, 2021 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-33706560

RESUMO

BACKGROUND: Inflammatory pain is the most common type of pain encountered clinically. The analgesic effect of acupuncture has been well-documented. OBJECTIVE: The aim of this study was to investigate the involvement of chemokine CXCL1 in the serum on manual acupuncture (MA)-induced antinociception. METHODS: Rats with inflammatory pain of the right hind paw were induced by intraplantar (i.pl.) administration of complete Freund's adjuvant (CFA). After wards, the CFA-injected rats were treated daily with MA at ST36 from Day 1 to Day 7, and thermal nociceptive thresholds (paw withdrawal latency; PWL) were analyzed. The concentration of CXCL1 in the serum of the rats was measured by enzyme-linked immunosorbent assay (ELISA) after the first and the last MA treatment. Subsequently, the rats were injected with two doses (5 or 10 µg) of recombinant CXCL1 through the tail vein daily from Day 1 to Day 7 or injected with two doses (6.4 or 16 µg) of anti-CXCL1 antibody using the same methods and course at 30 min before MA, and the PWLs were measured again. Finally, naloxone (500 µg, 0.1 mL) was administered by i.pl. injection into the inflamed paw 5 min before the last MA treatment or last injection of recombinant CXCL1. RESULTS: MA significantly increased the PWLs and upregulated the expression of serum CXCL1 in the CFA-injected rats. Without acupuncture, repeated tail vein injection of recombinant CXCL1 showed an analgesic effect on CFA-induced inflammatory pain. Conversely, the neutralization of serum CXCL1 by anti-CXCL1 antibody decreased MA-induced antinociception in a time-dependent manner. Anti-CXCL1 antibody injected just once before the first MA did not affect MA-induced antinociception. The analgesic effects of MA and recombinant CXCL1 were reversed by an i.pl. injection of naloxone. CONCLUSION: This study indicates MA at ST36 had an analgesic effect on inflammatory pain and found a novel function of CXCL1. Increased serum CXCL1 had an antinociceptive effect on inflammatory pain induced by CFA. CXCL1 in serum appeared to be a key molecule involved in the peripheral mechanism of MA-induced antinociception. The analgesic effect of MA or recombinant CXCL1 on inflammatory pain might be mediated through a peripheral opioid pathway, which needs further investigation.

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