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1.
Cell Death Dis ; 13(5): 433, 2022 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-35508523

RESUMO

The long non-coding RNA (lncRNA) taurine up-regulated gene 1 (TUG1) acts as tumor-promoting factor in colorectal cancer (CRC). We aimed to elucidate the mechanism by which the transcription factor specificity protein 1 (SP1) regulates TUG1 and microRNAs (miRs)/mRNAs in the context of CRC, which has not been fully studied before. Expression patterns of TUG1 and SP1 were determined in clinical CRC samples and cells, followed by identification of their interaction. Next, the functional significance of TUG1 in CRC was investigated. An in vivo CRC model was established to validate the effect of TUG1. The results demonstrated that TUG1 and SP1 were highly-expressed in CRC, wherein SP1 bound to the TUG1 promoter and consequently, positively regulated its expression. Silencing of TUG1 caused suppression of CRC cell growth and promotion of cell apoptosis. TUG1 could bind to miR-421 to increase KDM2A expression, a target gene of miR-421. TUG1 could activate the ERK pathway by impairing miR-421-targeted inhibition of KDM2A. Additionally, SP1 could facilitate the tumorigenesis of CRC cells in vivo by regulating the TUG1/miR-421/KDM2A/ERK axis. Altogether, the current study emphasizes the oncogenic role of TUG1 in CRC, and illustrates its interactions with the upstream transcription factor SP1 and the downstream modulatory axis miR-421/KDM2A/ERK, thus offering novel insights into the cancerogenic mechanism in CRC.


Assuntos
Neoplasias Colorretais , Proteínas F-Box , MicroRNAs , RNA Longo não Codificante , Carcinogênese/genética , Linhagem Celular Tumoral , Proliferação de Células/genética , Neoplasias Colorretais/genética , Neoplasias Colorretais/metabolismo , Proteínas F-Box/metabolismo , Regulação Neoplásica da Expressão Gênica/genética , Humanos , Histona Desmetilases com o Domínio Jumonji/metabolismo , MicroRNAs/genética , MicroRNAs/metabolismo , RNA Longo não Codificante/genética , RNA Longo não Codificante/metabolismo , Fator de Transcrição Sp1/genética , Fator de Transcrição Sp1/metabolismo , Fatores de Transcrição/metabolismo , Regulação para Cima/genética
2.
Front Bioeng Biotechnol ; 10: 866290, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35433668

RESUMO

Degenerative disc disease (DDD) is a pathological condition associated with intervertebral discs (IVDs) that causes chronic back pain. IVD degeneration has become a significant issue in contemporary society. To date, numerous biological therapies have been applied to alleviate the progression of DDD, among which therapeutic protein injection is the most direct and convenient. However, there are some limitations to applying direct protein injection therapy, the most significant being that the efficacy of this method has a short duration, which is a major factor in its effectiveness and the resulting patient satisfaction. How do we solve this problem? Or how can the effectiveness of the treatment be enhanced? It has been proved that manganese dioxide (MnO2) microspheres, widely used in environmental science, not only regulate the expression of cell genes and cytokines in the microenvironment, but also have the ability to release drugs slowly. We propose that direct injection of protein encapsulated in hollow MnO2 (h-MnO2) microspheres could solve the problem of rapid drug release. In addition, the use of a MnO2 and protein injection in the treatment of DDD may have a synergistic effect, which would be highly significant for the degradation of pro-inflammatory factors in the DDD microenvironment. Therefore, the combination of MnO2 and protein may provide a new therapeutic approach to alleviate the progression of DDD.

3.
J Orthop Translat ; 33: 41-54, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-35228996

RESUMO

BACKGROUND: Periosteum plays a significant role in bone formation and regeneration by storing progenitor cells, and also acts as a source of local growth factors and a scaffold for recruiting cells and other growth factors. Recently, tissue-engineered periosteum has been studied extensively and shown to be important for osteogenesis and chondrogenesis. Using biomimetic methods for artificial periosteum synthesis, membranous tissues with similar function and structure to native periosteum are produced that significantly improve the efficacy of bone grafting and scaffold engineering, and can serve as direct replacements for native periosteum. Many problems involving bone defects can be solved by preparation of idealized periosteum from materials with different properties using various techniques. METHODS: This review summarizes the significance of periosteum for osteogenesis and chondrogenesis from the aspects of periosteum tissue structure, osteogenesis performance, clinical application, and development of periosteum tissue engineering. The advantages and disadvantages of different tissue engineering methods are also summarized. RESULTS: The fast-developing field of periosteum tissue engineering is aimed toward synthesis of bionic periosteum that can ensure or accelerate the repair of bone defects. Artificial periosteum materials can be similar to natural periosteum in both structure and function, and have good therapeutic potential. Induction of periosteum tissue regeneration and bone regeneration by biomimetic periosteum is the ideal process for bone repair. CONCLUSIONS: Periosteum is essential for bone formation and regeneration, and it is indispensable in bone repair. Achieving personalized structure and composition in the construction of tissue engineering periosteum is in accordance with the design concept of both universality and emphasis on individual differences and ensures the combination of commonness and individuality, which are expected to meet the clinical needs of bone repair more effectively. THE TRANSLATIONAL POTENTIAL OF THIS ARTICLE: To better understand the role of periosteum in bone repair, clarify the present research situation of periosteum and tissue engineering periosteum, and determine the development and optimization direction of tissue engineering periosteum in the future. It is hoped that periosteum tissue engineering will play a greater role in meeting the clinical needs of bone repair in the future, and makes it possible to achieve optimization of bone tissue therapy.

4.
Stem Cell Res Ther ; 13(1): 70, 2022 02 11.
Artigo em Inglês | MEDLINE | ID: mdl-35148808

RESUMO

Intervertebral disc degeneration (IDD) is a common disease that increases with age, and its occurrence is stressful both psychologically and financially. Stem cell therapy for IDD is emerging. For this therapy, stem cells from different sources have been proven in vitro, in vivo, and in clinical trials to relieve pain and symptoms, reverse the degeneration cascade, delay the aging process, maintain the spine shape, and retain mechanical function. However, further research is needed to explain how stem cells play these roles and what effects they produce in IDD treatment. This review aims to summarize and objectively analyse the current evidence on stem cell therapy for IDD.


Assuntos
Degeneração do Disco Intervertebral , Disco Intervertebral , Humanos , Degeneração do Disco Intervertebral/terapia , Células-Tronco
5.
Nanoscale ; 14(7): 2649-2659, 2022 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-35134104

RESUMO

Developing a novel antibiotics-free antibacterial strategy is essential for minimizing bacterial resistance. Materials that not only kill bacteria but also promote tissue healing are especially challenging to achieve. Inspired by chemical conversion processes in living organisms, we develop a piezoelectrically active antibacterial device that converts ambient O2 and H2O to ROS by piezocatalytic processes. The device is achieved by mounting nanoscopic polypyrrole/carbon nanotube catalyst multilayers onto piezoelectric-dielectric films. Under stimuli by a hand-held massage device, the sterilizing rates for S. aureus and E. coli reach 84.11% and 94.85% after 10 minutes of operation, respectively. The antibacterial substrate at the same time preserves and releases drugs and presents negligible cytotoxicity. Animal experiments demonstrate that daily treatment for 10 minutes using the device effectively accelerates the healing of infected wounds on the backs of mice, promoting hair follicle generation and collagen deposition. We believe that this report provides a novel design approach for antibacterial strategies in medical treatment.


Assuntos
Nanocompostos , Staphylococcus aureus , Animais , Antibacterianos/química , Bandagens , Escherichia coli , Camundongos , Nanocompostos/química , Polímeros/farmacologia , Pirróis
6.
Int J Nanomedicine ; 17: 45-60, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35027826

RESUMO

INTRODUCTION: Modulating the inflammatory response of human gingival fibroblasts (hGFs) is important for the control of periodontal inflammation because it is a key event in the pathogenesis of periodontitis. Here, we aimed to determine whether polyglucose sorbitol carboxymethyl ether (PSC)-coated superparamagnetic iron oxide nanoparticles (SPIONs) protect hGFs against invasion and inflammatory stimulation by Porphyromonas gingivalis (P. gingivalis). METHODS: First, we determined the cytotoxicity and antimicrobial activity of PSC-SPIONs. Then, their effects on invasion of hGFs by P. gingivalis were evaluated by counting invading P. gingivalis, fluorescence staining, and transmission electron microscopy. The effect of PSC-SPIONs on inflammation in hGFs induced by P. gingivalis lipopolysaccharide was evaluated by measurement of reactive oxygen species (ROS), and enzyme-linked immunosorbent assays, quantitative reverse transcription-polymerase chain reaction, and Western blotting of key indicator molecules. The effects of dimercaptosuccinic acid (DMSA)-coated SPIONs and the free form of PSC alone were also tested and compared with those of PSC-SPIONs. RESULTS: PSC-SPIONs (25 µg/mL) are cytocompatible with hGFs and exhibit no antimicrobial effects on P. gingivalis. However, they inhibit invasion of hGFs by P. gingivalis at 15 µg/mL. They also decrease ROS production and inflammatory cytokine secretion by hGFs at 5, 15, and 25 µg/mL, by downregulating activation of the nuclear factor-kappa B signaling pathway. Furthermore, PSC alone does not inhibit inflammation, while DMSA-SPIONs do. This indicates that the nanosize effects of PSC-SPIONs, rather than their coating material, play the dominant role in their anti-inflammatory activity. CONCLUSION: PSC-SPIONs protect hGFs against P. gingivalis invasion and inflammatory stimulation. Thus, they have potential for clinical application in control of periodontal inflammation.


Assuntos
Gengiva , Porphyromonas gingivalis , Células Cultivadas , Fibroblastos , Humanos , Lipopolissacarídeos/farmacologia
7.
ACS Nano ; 16(2): 2968-2977, 2022 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-35043616

RESUMO

The poor ionic conductivity of transition metal oxides (TMOs) is a huge obstacle to their practical application as anodes for lithium-ion batteries (LIBs). Although good performance can be harvested by constructing nanostructures, some other foundmental issues including low tap density and serious electrolyte consumption come along. Herein, inspired by frogspawn, we propose a universal strategy of using lithium salts to assemble TMO nanoparticles into large aggregates to improve their Li+ conductivity. In such a frogspawn-like structure, lithium salt networks can not only realize the rapid transmission of Li+ but also alleviate the volume change during the charging/discharging process. When Li3PO4 is applied to assemble iron oxides nanoparticles, aggregates with size over 1 µm and tap density up to 1.33 g cm-3 can be obtained, which even hasve an ionic conductivity up to 9.61 × 10-5 S cm-1. Fe3O4 was also introduced through reduction to boost electron transfer. Consequently, this carbon-free composite delivered a capacity up to 896 mA h g-1 even after 1000 cycles at 5 A g-1, which can also be maintained under high mass loading. When using lithium salts such as Li2SO4, Li2CO3, LiBO2, and LiCl, the corresponding composites also showed similar performance. This strategy is also effective for TMOs such as NiO, Co3O4, and ZnO, demonstrating the universality of this frogspawn-inspired design.

8.
ACS Appl Mater Interfaces ; 13(50): 60063-60071, 2021 Dec 22.
Artigo em Inglês | MEDLINE | ID: mdl-34889603

RESUMO

The interfacial charge storage is derived from spin-polarized electrons stored on the surface of iron metal nanoparticles, and reasonable utilization can achieve a capacity far beyond the traditional conversion mechanism. Generally, iron oxide is easy to crack, pulverize, and fall off due to its poor conductivity and large volume change during cycling, and causes serious side reactions with the electrolyte. Herein, this pulverization phenomenon was intentionally utilized to in situ form nano-sized iron particles and create a large number of Fe/Li2O interfaces. Specifically, a Li+ conductor like Li2SO4 was utilized to seal micron sized iron oxides and also work as an aggregation barrier. Thus, the in situ formed nanoparticles were separated from the electrolyte and could provide huge capacity through interfacial charge storage. Therefore, the specific capacity of this unique composite continues to rise upon activation cycling and finally reaches 1708 mA h g-1, which is more than twice its theoretical capacity based on the conversion mechanism. The gradually increasing interfacial charge storage capacity was also directly confirmed by X-ray photoelectron spectroscopy tests. This novel strategy provides new opportunities for the design and commercialization of advanced energy storage systems.

9.
J Mater Sci Mater Med ; 32(9): 107, 2021 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-34427778

RESUMO

OBJECTIVE: To study the bone induction and defect repair of true bone ceramics (TBC) combined with rhBMP-2 and Sr. METHODS: MC3T3-E1 cells were used to evaluate the bioactivity of the composite. Cell proliferation activity was detected by CCK-8, ALP activity was detected by p-nitrophenyl phosphate (PNPP), and the differences of material surface topography were observed by scanning electron microscopy (SEM). Bone induction was verified by the implantation in nude mice. The rabbit femoral condyle defect model was achieved to verify the bone defect repair ability of the material. RESULTS: SEM results showed nearly the same surface morphology and cell proliferation quantified by CCK-8 showed that compared with TBC, both TBC&Sr and TBC&BMP-2&Sr had a significant promoting effect (P < 0.05). ALP activity result showed that the ALP activity of TBC&BMP-2&Sr was significantly higher than that of TBC alone (P < 0.05). The bone induction result showed that TBC&Sr had a small amount of new bone formation, and the new bone area was only 2.5 ± 0.11%. The bone induction activity of TBC&BMP-2&Sr was the highest, the new bone area was up to 75.36 ± 4.21%. Histological result of bone defect repair showed that TBC&BMP-2&Sr was also the highest, the new bone area was up to 72.42 ± 3.14%. The repair effect of TBC& BMP-2 was second, and better than that of TBC&Sr. CONCLUSION: TBC combined with rhBMP-2 and Sr had the good bioactivity, obvious bone conduction and bone defect repair performance, laying the foundation of clinical application potentially.


Assuntos
Proteína Morfogenética Óssea 2/farmacologia , Regeneração Óssea/efeitos dos fármacos , Osteogênese/efeitos dos fármacos , Estrôncio/farmacologia , Fator de Crescimento Transformador beta/farmacologia , Animais , Proteína Morfogenética Óssea 2/química , Substitutos Ósseos/química , Substitutos Ósseos/farmacologia , Osso e Ossos/citologia , Osso e Ossos/efeitos dos fármacos , Osso e Ossos/fisiologia , Diferenciação Celular/efeitos dos fármacos , Células Cultivadas , Cerâmica/química , Cerâmica/farmacologia , Materiais Revestidos Biocompatíveis/química , Materiais Revestidos Biocompatíveis/farmacologia , Feminino , Fraturas Ósseas/terapia , Masculino , Teste de Materiais , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Coelhos , Proteínas Recombinantes/química , Proteínas Recombinantes/farmacologia , Estrôncio/química , Tecidos Suporte/química , Fator de Crescimento Transformador beta/química
10.
J Nutr ; 151(9): 2601-2609, 2021 09 04.
Artigo em Inglês | MEDLINE | ID: mdl-34091674

RESUMO

BACKGROUND: Methylglyoxal (MGO), a precursor of advanced glycation end products (AGEs), has been linked to AGEs-associated diseases. OBJECTIVES: This study investigated the efficacy and mechanisms of dietary quercetin in decreasing plasma and tissue concentrations of MGO and AGEs in MGO-administered mice. METHODS: Male, 6-wk-old CD-1 mice were administered AIN-93G diet and water (Con) or 0.12% MGO in water (MGO) or MGO plus 0.2% (0.2Q) dietary quercetin for 1 wk (n = 5) (experiment 1), and water (Con), 0.12% MGO (MGO), or MGO plus 0.1% (0.1Q), 0.2% (0.2Q), or 0.4% (0.4Q) dietary quercetin for 6 wk (n = 10) (experiment 2). The plasma, kidney, and liver concentrations of MGO, quercetin, and isorhamnetin and their trapping adducts with MGO were determined by LC-MS, and AGE concentrations were measured by the fluorescent method. Furthermore, the expressions of glyoxalase I/II (GLO I/II) and aldose reductase (AR), MGO detoxification enzymes, were determined by Western blot. One-factor ANOVA and post hoc Dunnett's or Tukey's test were used to analyze the data. RESULTS: After 1 wk of treatment, the MGO concentrations in plasma (20.2%) and kidney (29.9%) in 0.2Q mice were significantly lower than those in MGO mice. After 6 wk of treatment, the concentrations of MGO in the plasma (14.7-18.6%), kidney (20-20.8%), liver (15.4-18.6%), and tissue AGEs (28-36.8%) in 0.1Q, 0.2Q, and 0.4Q mice were significantly lower than those in MGO mice. The plasma concentrations of quercetin, isorhamnetin, and their MGO adducts were dose-dependently increased after quercetin administration. In addition, after 6 wk of quercetin administration, the expressions of GLO I/II and AR in the liver and kidney were significantly upregulated to promote MGO detoxification compared with MGO-treated mice. CONCLUSIONS: Quercetin reduced plasma and tissue MGO concentrations and inhibited AGE formation by trapping MGO and regulating the MGO detoxification systems in MGO-administered healthy mice.


Assuntos
Produtos Finais de Glicação Avançada , Lactoilglutationa Liase , Aldeído Pirúvico , Animais , Dieta , Masculino , Camundongos , Quercetina
11.
Alzheimers Dement (Amst) ; 13(1): e12213, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34136637

RESUMO

INTRODUCTION: Despite increasing dementia rates, few culturally informed cognitive assessment tools exist for Indigenous populations. The Canadian Indigenous Cognitive Assessment (CICA) was adapted with First Nations on Manitoulin Island, Canada, and provides a brief, multi-domain cognitive assessment in English and Anishinaabemowin. METHODS: Using community-based participatory research (CBPR) methods, we assessed the CICA for inter-rater and test-retest reliability in 15 individuals. We subsequently evaluated validity and established meaningful CICA cut-off scores in 55 individuals assessed by a geriatrician. RESULTS: The CICA demonstrated strong reliability (intra-class coefficient = 0.95 [0.85,0.98]). The area under the curve (AUC) was 0.98 (0.94, 1.00), and the ideal cut-point to identify likely cases of dementia was a score of less than or equal to 34 with sensitivity of 100% and specificity of 85%. DISCUSSION: When used with older First Nations men and women living in First Nations communities, the CICA offers a culturally safe, reliable, and valid assessment to support dementia case-finding.

12.
Neuroimmunomodulation ; 28(4): 266-275, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33951651

RESUMO

BACKGROUND: The major event in the development of diabetes-related blindness and vision impairment is the onset of retinal cell damage. Overall awareness of insulin-like growth factor-2 (IGF2) mechanisms emphasizes its protective behavior in retinal cells that help to provide new information about the development of treatment for retinal complications. OBJECTIVES: This study analyzes the effect of in vitro changes associated with the cell survival and rescue mechanism in IGF2 inhibition and activation using chromeceptin and IGF2 peptides in ARPE-19 cells cultured in high glucose conditions. METHOD: Cell death was induced using high glucose (15 mmol/L), IGF2 inhibition was done using chromeceptin (1 µM) (Sigma Aldrich, Saint Louis, MO, USA), and IGF2 activation was done using IGF2 peptide (10 ng/mL). The cells were analyzed for changes in cell proliferation, apoptosis markers, antioxidant molecules, and alteration of cytokines. RESULTS: The study demonstrated that cells lacking IGF2 exhibited a significant increase in reactive oxygen levels with apoptosis patterns. Also, gene expression analysis by qRT-PCR demonstrated a significant increase in Yes-associated protein 1, CDK2, TNF-α, and BIRC5 genes in cells under high glucose stress and IGF inhibition compared to control. Further, the cytokine analysis also revealed that cells devoid of IGF2 activated an increase in cytokines such as IL-8, CX43, ICAM-1, IL-17, CCL3, and MCP-1 and decreased paraoxonase compared to normal control cells. On the other hand, ARPE-19 cells grown in high glucose shows that IGF2 increases the survival genes with reduced levels of inflammatory cytokines. CONCLUSION: The finding of the investigation, therefore, shows that the use of IGF2 activators may prevent the progression of ocular dysfunction in the control of diabetes-related complications.


Assuntos
Diabetes Mellitus , Retinopatia Diabética , Apoptose , Proliferação de Células , Retinopatia Diabética/tratamento farmacológico , Humanos , Neurônios , Retina
13.
Nanoscale ; 13(18): 8481-8489, 2021 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-33908572

RESUMO

Self-powered piezoelectrically active molecular or protein delivery devices have provoked great interest in recent years. However, electric fields used to promote delivery or healing may also induce the redox of water or oxygen to generate reactive oxygen species (ROS) and bring unintended oxidative pressure to the organism and harm biological functions. In addition, protein molecules are easily inactivated in the polymer reservoir matrix due to the pull of strong electrostatic effects. In this study, a multifunctional molecular delivery substrate was fabricated by integrating a piezoelectric-dielectric polymeric substrate, nanoscopic polyelectrolyte films and in-film deposited biomimetic porous CaP coating. The piezoelectric substrate promoted molecular release, and the mineralized coating effectively stored molecules or proteins and simultaneously eliminated ROS, reducing the oxidative stress response generated by oxidative pressure. The present work opens a new way for the development of multifunctional and biofriendly drug delivery devices.


Assuntos
Motivação , Polímeros , Sistemas de Liberação de Medicamentos , Estresse Oxidativo , Espécies Reativas de Oxigênio
14.
J Orthop Translat ; 28: 74-82, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33738240

RESUMO

BACKGROUND: Xenograft bone scaffolds have advantages such as mechanical strength, sufficient source and safety. Combined with siRNA properly targeting CKIP-1, a negative regulator of osteogenesis, may contribute to the repair result of calcine bone alone. METHODS: Herein, we constructed a novel xenograft bovine bone scaffold namely (DSS)6-liposome/CKIP-1 siRNA/calcine bone, the characteristics of which were investigated by confirming the effect of (DSS)6-liposome, observing the appearance and testing mechanical strength of calcine bone, and observing the combined result of CKIP-1 siRNA by FAM immunofluorescence. In addition, cytotoxicity by CCK-8 and LDH activity of L929 â€‹cells and MC3T3-E1 osteoblasts cultured with the scaffold were tested in vitro, primary osteoblasts proliferation, the mRNA expressions of CKIP-1, ALP, COL1-α and OCN, the protein expressions of CKIP-1, BMP-2, COL-1 and Runx2 and calcium nodules were also determined by CCK-8, RT-qPCR, western-blot and Alizarin Red staining in vitro. Then, we successively established the skull defect model for evaluating the repair result of the novel scaffold by HE staining of 2, 4, 8 and 12 weeks, immumohistochemical stainings of 2, 4, 8 and 12 weeks such as ALP, COL-1α and OCN, Mirco-CT scanning of 4 and 12 weeks and the relative parameters and so on in vivo. RESULTS: It indicated that (DSS)6-liposome/CKIP-1 siRNA/calcine bone could successfully knock down the CKIP-1 mRNA and protein expressions, promote osteoblasts proliferation with the little cytotoxicity in vitro, increase the protein expressions of BMP-2, COL-1 and Runx2 in vitro, increase mRNA expressions of ALP, COL-1α and OCN in vitro and in vivo, and have a better bone defect repair effect with few side effects in rats after 12 weeks. CONCLUSION: Our research indicates (DSS)6-liposome/CKIP-1 siRNA/calcine bone could repair skull defects well in rats, and it may lay the foundation of applicating the novel xenograft bone scaffold in the clinical. THE TRANSLATIONAL POTENTIAL OF THIS ARTICLE: These findings provide evidence that (DSS)6- liposome/CKIP-1 siRNA/calcine bone could be used as a novel xenograft bone scaffold for osteogenesis with the good safety.

15.
ACS Biomater Sci Eng ; 7(4): 1302-1337, 2021 04 12.
Artigo em Inglês | MEDLINE | ID: mdl-33764038

RESUMO

Alginate is a natural polysaccharide that is easily chemically modified or compounded with other components for various types of functionalities. The alginate derivatives are appealing not only because they are biocompatible so that they can be used in biomedicine or tissue engineering but also because of the prospering bioelectronics that require various biomaterials to interface between human tissues and electronics or to serve as electronic components themselves. The study of alginate-based materials, especially hydrogels, have repeatedly found new frontiers over recent years. In this Review, we document the basic properties of alginate, their chemical modification strategies, and the recent development of alginate-based functional composite materials. The newly thrived functions such as ionically conductive hydrogel or 3D or 4D cell culturing matrix are emphasized among other appealing potential applications. We expect that the documentation of relevant information will stimulate scientific efforts to further develop biocompatible electronics or smart materials and to help the research domain better address the medicine, energy, and environmental challenges faced by human societies.


Assuntos
Alginatos , Hidrogéis , Materiais Biocompatíveis , Eletrônica , Humanos , Engenharia Tecidual
16.
ISA Trans ; 117: 180-195, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33581891

RESUMO

The content of free calcium oxide (f-CaO) in cement clinker is an important index for cement quality. Aiming at the characteristics of strong coupling, time-varying delay and highly non-linearity in cement clinker production, a soft sensor model based on multivariate time series analysis and convolutional neural network (MVTS-CNN) is proposed for the online f-CaO content monitoring. Based on the process industry characteristics, the MVTS-CNN modeling involves the detailed analysis of coupling relationship and time-varying delay in cement production and the application of neural network in multivariate time-series feature extraction. The main researches and contributions are fourfold: First, the strong coupling in the production system is further analyzed, and the proposed model is focused on the data coupling between specific processes, not the control coupling. Second, a multivariate time series analysis method is designed to select the time series that may have direct impacts on the f-CaO content in different production conditions, which is founded on the information on time delay range and longest active duration. Third, a multivariate time series feature extraction method is designed and adopted in the MVTS-CNN model to extract the multivariate time series features, such as active duration difference features, coupling features, nonlinear features and key time series features. Fourth, a new timing matching method, which is combined the rough timing matching of multivariate time series and the detailed timing matching of key features, is proposed to deal with the time-varying delay in various production conditions. Compared with traditional CNN, support vector machines (SVM) and long-short term memory networks (LSTM), the results demonstrate that the MVTS-CNN model has higher accuracy, better generalization ability and superior robustness.

17.
J Agric Food Chem ; 69(3): 1123-1131, 2021 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-33464893

RESUMO

As potential endogenous biomarkers, reactive carbonyl species (RCS) have gained abundant attention for monitoring oxidative and carbonyl stress. However, there is no accurate method to evaluate multiple RCS in biological samples. In this study, a 2,4-dinitrophenylhydrazine (DNPH) derivatization-based LC-MS method was developed and validated to quantitate eight RCS: malondialdehyde (MDA), acrolein (ACR), 4-hydroxy-2-nonenal (4-HNE), 4-oxo-2-nonenal (4-ONE), methylglyoxal (MGO), glyoxal (GO), 3-deoxyglucosone (3-DG), and 2-keto-d-glucose (2-Keto). Subsequently, the method was applied to assess the RCS in low fat (LF), high fat (HF), and HF plus rosemary extract (RE) diet-fed mouse samples. The quantitative results on RCS levels indicated that the HF diet significantly increased the total RCS levels in mouse urine, plasma, and kidney with an average rate of 280.69%, 153.87%, and 61.30%, respectively. The RE administration significantly inhibited the elevated RCS levels induced by the HF diet, especially for MDA, 4-ONE, 4-HNE, and 2-Keto in mouse plasma, and ACR and 2-Keto in mouse kidney. This is the first study to simultaneously measure eight RCS in biological samples and demonstrate that RE was able to eliminate the accumulation of the HF diet-induced RCS.


Assuntos
Aldeídos/metabolismo , Doenças Metabólicas/tratamento farmacológico , Extratos Vegetais/administração & dosagem , Rosmarinus/química , Aldeídos/química , Animais , Dieta Hiperlipídica/efeitos adversos , Humanos , Masculino , Doenças Metabólicas/etiologia , Doenças Metabólicas/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Estresse Oxidativo
18.
J Craniofac Surg ; 32(1): e90-e92, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-32675768

RESUMO

PURPOSE: The aim of this study was to evaluate the treatment strategy of open reduction and internal fixation (ORIF) for comminuted mandibular fracture (CMF). METHODS: Clinical studies about CMF were collected. Detailed information was extracted, and data were analyzed and merged from included articles. RESULTS: Twelve studies, including 338 patients with CMF, were reported. A total of 256 patients receive ORIF among these 338 patients, and exhibited followed characteristics: ORIF usually were performed several days after injury; the extraoral approach for ORIF was used for 103 patients among 205 patients who received ORIF with definite information about surgical approach; titanium mesh, or reconstruction plate, combined with mini-plates was used in 17 and 194 patients, respectively; intermaxillary fixation (IMF) usually persisted about 1 to 3 weeks after ORIF; most patients exhibited satisfactory effect without serious complications, and the complication rate varied from 0 to 42%. CONCLUSIONS: ORIF strategy for treatment of CMF including: ORIF was a priority choice for CMF. ORIF usually was performed at several days after injury. Reconstruction plate, or titanium mesh, combined with mini-plates was recommended for ORIF surgery. After ORIF, IMF usually was recommended for about 1 to 3 weeks.


Assuntos
Fraturas Cominutivas , Fraturas Mandibulares , Placas Ósseas , Fixação de Fratura , Fixação Interna de Fraturas , Fraturas Cominutivas/cirurgia , Humanos , Fraturas Mandibulares/cirurgia , Redução Aberta , Estudos Retrospectivos , Resultado do Tratamento
19.
ACS Appl Mater Interfaces ; 13(1): 257-265, 2021 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-33378174

RESUMO

Controllable drug release is promising for fighting against antimicrobial resistance, which is a critical threat to human health worldwide. Herein, new hyaluronidase-responsive conjugated oligo(thiophene ethynylene) (OTE)-covalently modified hyaluronic acid (OTE-HA) nanoparticles for on-demand release of antimicrobial agents are reported. The synthesis of amphiphilic OTE-HA was carried out by esterification reaction. The resulting macromolecules were self-assembled in water to form nanoparticles, in which the hydrophobic OTE section, as bactericides, formed "cores" and the hydrophilic hyaluronic acid (HA) formed "shells". The OTE-HA nanoparticles avoid bactericide premature leakage and effectively block the dark cytotoxicity of the OTE section, possessing excellent biocompatibility. Using methicillin-resistant Staphylococcus aureus (MRSA) as an example, hyaluronidase, largely secreted by MRSA, can in situ trigger the release of OTE via hydrolyzing OTE-HA nanoparticles into fragments, even disaccharides linked with OTE. Importantly, the OTE section could effectively break cell membranes, leading to bacterial death. The half-maximal inhibitory concentration of the nanoparticles against MRSA is 3.3 µg/mL. The great antibacterial activity of OTE-HA nanoparticles against Gram-positive bacteria Streptococcus pneumoniae further confirms the controllable bactericide delivery mechanism. OTE-HA nanoparticles coated on a surface can also effectively inhibit the growth of bacteria, which holds a remarkable promise in biomedical applications. Therefore, this work provides a favorable strategy of on-demand and in situ drug release for sterilization and defeating antimicrobial resistance.


Assuntos
Antibacterianos/farmacologia , Portadores de Fármacos/química , Ácido Hialurônico/química , Nanopartículas/química , Compostos de Amônio Quaternário/farmacologia , Tiofenos/farmacologia , Células A549 , Antibacterianos/síntese química , Antibacterianos/metabolismo , Proteínas de Bactérias/metabolismo , Portadores de Fármacos/síntese química , Portadores de Fármacos/toxicidade , Desenho de Fármacos , Liberação Controlada de Fármacos , Farmacorresistência Bacteriana/efeitos dos fármacos , Humanos , Ácido Hialurônico/síntese química , Ácido Hialurônico/toxicidade , Hialuronoglucosaminidase/metabolismo , Hidrólise , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Staphylococcus aureus Resistente à Meticilina/enzimologia , Testes de Sensibilidade Microbiana , Nanopartículas/toxicidade , Compostos de Amônio Quaternário/síntese química , Compostos de Amônio Quaternário/metabolismo , Streptococcus pneumoniae/efeitos dos fármacos , Streptococcus pneumoniae/enzimologia , Tiofenos/síntese química , Tiofenos/metabolismo
20.
ACS Biomater Sci Eng ; 6(10): 5785-5796, 2020 10 12.
Artigo em Inglês | MEDLINE | ID: mdl-33320584

RESUMO

Nerves spread throughout human bone minerals and play an important role in regulating osteogenic homeostasis. However, whether the distributive nerves can sense bone minerals and the role of bone minerals in nerve outgrowth are still unclear. We hypothesized that a natural magnesium-containing bone mineral, whitlockite (WH), the second most abundant bone mineral in the human body, could simultaneously promote both osteogenic and neural activities. To verify the hypothesis, both WH and hydroxyapatite (HAP) nanoparticles were synthesized, and their characterization was completed by Fourier-transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD). The effect of WH on neural differentiation of mesenchymal stem cells (MSCs) and neural progenitor cells (NPCs) in 2D and 3D culture was examined by immunostaining and quantitative real-time polymerase chain reaction (qRT-PCR). The secretion of calcitonin gene-related polypeptide (CGRP) was examined by enzyme-linked immunosorbent assay (ELISA). The neural and osteogenic differentiation in a preosteoblasts and NPCs coculture system was examined by immunostaining and qRT-PCR. The results showed that WH promotes the gene and protein expression of neuronal specific marker (MAP-2 and ßIII-tubulin) in 2D and 3D culture systems. In addition, the neurite length in the WH group was significantly longer than in other groups. Furthermore, both neural differentiation and osteogenic differentiation were simultaneously enhanced in the WH group in the coculture system. Thus, this study demonstrated the simultaneous stimulatory effect of WH bone mineral on neural and osteogenic activities, which provided WH as a valuable material for bone regeneration.


Assuntos
Magnésio , Osteogênese , Fosfatos de Cálcio , Humanos , Magnésio/farmacologia , Espectroscopia de Infravermelho com Transformada de Fourier
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