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1.
Ying Yong Sheng Tai Xue Bao ; 30(3): 923-930, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30912385

RESUMO

Understanding the responses of stomatal structure, photosynthesis and biomass of maize to exogenous Ca2+ addition under NaCl stress has important significance for further uncovering the alleviative mechanism of exogenous Ca2+ on maize under salt stress. We examined the effects of exogenous Ca2+(0, 5, 10, 20, 40, 80 mmol·L-1) on the stomatal structure, photosynthesis and biomass of maize (Zea mays L. cv. Jingke 665) seedlings under NaCl stress (100 mmol·L-1). Our results showed that exogenous Ca2+ addition had limited effect on stomatal density, but significantly decreased stomatal shape index, stomatal area, stomatal length, stomatal width, and stomatal cir-cumference. Meanwhile, the net photosynthetic rate (Pn) initially increased and then decreased with the increases of exogenous Ca2+ concentration, whereas both the stomatal conductance (gs) and intercellular CO2 concentration (Ci) were decreased, suggesting that the decrease of Pn was mainly due to stomatal limitation under high Ca2+ concentration. The biomass of maize seedlings was increased and the root/shoot ratio was decreased with the increases of exogenous Ca2+ concentration, suggested that the alleviated effect of exogenous Ca2+ on aboveground biomass was higher than that on belowground biomass of maize under salt stress.


Assuntos
Fotossíntese , Zea mays , Biomassa , Cálcio , Folhas de Planta , Estresse Salino , Plântula
2.
Front Pharmacol ; 9: 1031, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30283336

RESUMO

Alcoholic liver disease (ALD) is a complex process that includes a wide range of hepatic lesions, from steatosis to cirrhosis, and even hepatocellular carcinoma (HCC). Accumulating evidence shows that the cytotoxic effects of ethanol metabolism lead to cell apoptosis and necrosis in ALD. Recently, several studies revealed that multifunctional protein ß-arrestin 2 (Arrb2) modulated cell apoptosis in liver fibrosis and HCC, but its role in ALD has not been fully understood. The aim of this study is to explore the function and underlying mechanism of Arrb2 in hepatocyte survival and apoptosis in ALD. In our study, the primary hepatocytes were isolated from the livers of C57BL/6 mice fed EtOH-containing diet, it showed an increased level of Arrb2. EtOH also significantly up-regulated Arrb2 production in AML-12 cells in vitro. Furthermore, TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) and FCM results demonstrated that knockdown of Arrb2 could inhibit hepatocyte apoptosis induced by EtOH in vivo and vitro while over-expression of Arrb2 induced apoptosis in ALD. In addition, western blot results revealed that Arrb2 remarkably suppressed the Akt signaling. Taken together, our data suggested that Arrb2 may serve as a potential therapeutic target for ALD by promoting hepatocyte apoptosis via Akt suppression.

3.
Cell Cycle ; 17(2): 250-262, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29251244

RESUMO

ABSTRAT Alcoholic liver disease (ALD) and its complication continued to be a major health problem throughout the world. Increasing evidence suggests that microRNA (miRNA) that regulate apoptosis, inflammation and lipid metabolism are affected by alcohol in ALD. MiR-200a has emerged as a major regulator in several liver diseases, but its role in ALD has not been elucidated. The aim of this study is to figure out the biological function of miR-200a in ALD and to explore its underlying mechanism. The expression pattern of miR-200a were analyzed in vitro and in vivo, we showed that miR-200a was up-regulated in ALD in AML-12 and primary hepatocyte. We then examined it's effect on cell apoptosis and identified zinc finger E-box binding homeobox 2 (ZEB2; also known as SIP1) as a direct target gene of miR-200a. Furthermore, reintroduction of ZEB2 could reverse the pro-apoptosis of miR-200a on AML-12. Taken together, our study demonstrated that miR-200a regulates the apoptosis of hepatocyte in ALD by directly target ZEB2, both of which could serve as new therapeutic targets for ALD.


Assuntos
Apoptose , Hepatopatias Alcoólicas/genética , MicroRNAs/metabolismo , Homeobox 2 de Ligação a E-box com Dedos de Zinco/genética , Animais , Linhagem Celular , Etanol/toxicidade , Hepatócitos/citologia , Hepatócitos/efeitos dos fármacos , Hepatócitos/metabolismo , Hepatopatias Alcoólicas/metabolismo , Hepatopatias Alcoólicas/patologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Homeobox 2 de Ligação a E-box com Dedos de Zinco/metabolismo
4.
Clin Res Hepatol Gastroenterol ; 41(5): 585-591, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28918932

RESUMO

PURPOSE: To discuss the value of the Bedside Index for Severity in Acute Pancreatitis (BISAP), Modified Early Warning Score (MEWS), serum Ca2+, similarly hereinafter, and red cell distribution width (RDW) for predicting the severity grade of acute pancreatitis and to develop and verify a more accurate scoring system to predict the severity of AP. METHODS: In 302 patients with AP, we calculated BISAP and MEWS scores and conducted regression analyses on the relationships of BISAP scoring, RDW, MEWS, and serum Ca2+ with the severity of AP using single-factor logistics. The variables with statistical significance in the single-factor logistic regression were used in a multi-factor logistic regression model; forward stepwise regression was used to screen variables and build a multi-factor prediction model. A receiver operating characteristic curve (ROC curve) was constructed, and the significance of multi- and single-factor prediction models in predicting the severity of AP using the area under the ROC curve (AUC) was evaluated. The internal validity of the model was verified through bootstrapping. RESULTS: Among 302 patients with AP, 209 had mild acute pancreatitis (MAP) and 93 had severe acute pancreatitis (SAP). According to single-factor logistic regression analysis, we found that BISAP, MEWS and serum Ca2+ are prediction indexes of the severity of AP (P-value<0.001), whereas RDW is not a prediction index of AP severity (P-value>0.05). The multi-factor logistic regression analysis showed that BISAP and serum Ca2+ are independent prediction indexes of AP severity (P-value<0.001), and MEWS is not an independent prediction index of AP severity (P-value>0.05); BISAP is negatively related to serum Ca2+ (r=-0.330, P-value<0.001). The constructed model is as follows: ln()=7.306+1.151*BISAP-4.516*serum Ca2+. The predictive ability of each model for SAP follows the order of the combined BISAP and serum Ca2+ prediction model>Ca2+>BISAP. There is no statistical significance for the predictive ability of BISAP and serum Ca2+ (P-value>0.05); however, there is remarkable statistical significance for the predictive ability using the newly built prediction model as well as BISAP and serum Ca2+ individually (P-value<0.01). Verification of the internal validity of the models by bootstrapping is favorable. CONCLUSION: BISAP and serum Ca2+ have high predictive value for the severity of AP. However, the model built by combining BISAP and serum Ca2+ is remarkably superior to those of BISAP and serum Ca2+ individually. Furthermore, this model is simple, practical and appropriate for clinical use.


Assuntos
Pancreatite/diagnóstico , Doença Aguda , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Modelos Estatísticos , Pancreatite/sangue , Valor Preditivo dos Testes , Prognóstico , Estudos Retrospectivos , Índice de Gravidade de Doença
5.
Zhongguo Zhong Yao Za Zhi ; 42(13): 2619-2622, 2017 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-28840708

RESUMO

To improve the administration of local crude drug quality standard, the administration history, and current administration situation of local crude drugs were reviewed, the legal orientation and positive effect of local crude drugs were analyzed, and the existing problems were summarized. It was found that many problems existed in the administration of local crude drug quality standards, especially the phenomenon of homonym and synonym on their names. The suggestions on improving the administration of local crude drug quality standards were proposed. First of all, the construction of legal system should be strengthened to improve the administration methods. Secondly, the coordination mechanism should be developed to solve the outstanding problems. Thirdly, the basic research should be enhanced to resolve the general technical problems. Lastly, the channels to transfer the local crude drugs into pharmacopeia standards should be developed to achieve dynamic administration.


Assuntos
Preparações Farmacêuticas/normas , Controle de Qualidade , Medicina Tradicional Chinesa , Padrões de Referência
6.
J Cell Mol Med ; 21(10): 2317-2328, 2017 10.
Artigo em Inglês | MEDLINE | ID: mdl-28332284

RESUMO

EZH2, a histone H3 lysine-27-specific methyltransferase, is involved in diverse physiological and pathological processes including cell proliferation and differentiation. However, the role of EZH2 in liver fibrosis is largely unknown. In this study, it was identified that EZH2 promoted Wnt pathway-stimulated fibroblasts in vitro and in vivo by repressing Dkk-1, which is a Wnt pathway antagonist. The expression of EZH2 was increased in CCl4 -induced rat liver and primary HSCs as well as TGF-ß1-treated HSC-T6, whereas the expression of Dkk1 was reduced. Silencing of EZH2 prevented TGF-ß1-induced proliferation of HSC-T6 cells and the expression of α-SMA. In addition, knockdown of Dkk1 promoted TGF-ß1-induced activation of HSCs. Moreover, silencing of EZH2 could restore the repression of Dkk-1 through trimethylation of H3K27me3 in TGF-ß1-treated HSC-T6 cells. Interestingly, inhibition of EZH2 had almost no effect on the activation of HSC when Dkk1 was silenced. Collectively, EZH2-mediated repression of Dkk1 promotes the activation of Wnt/ß-catenin pathway, which is an essential event for HSC activation.


Assuntos
Proteína Potenciadora do Homólogo 2 de Zeste/metabolismo , Células Estreladas do Fígado/metabolismo , Peptídeos e Proteínas de Sinalização Intercelular/metabolismo , Cirrose Hepática/metabolismo , Animais , Tetracloreto de Carbono , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Proliferação de Células/genética , Células Cultivadas , Proteína Potenciadora do Homólogo 2 de Zeste/genética , Regulação da Expressão Gênica , Células Estreladas do Fígado/efeitos dos fármacos , Peptídeos e Proteínas de Sinalização Intercelular/genética , Cirrose Hepática/induzido quimicamente , Cirrose Hepática/genética , Interferência de RNA , Ratos Sprague-Dawley , Fator de Crescimento Transformador beta1/farmacologia , Via de Sinalização Wnt/efeitos dos fármacos , Via de Sinalização Wnt/genética
7.
Med Sci Monit ; 23: 665-672, 2017 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-28163298

RESUMO

BACKGROUND The aim of this study was to investigate the correlation between Hector Battifora mesothelial-1 (HBME-1) expression and the clinical pathological characteristics and prognosis of osteosarcoma (OS). MATERIAL AND METHODS HBME-1 expression was assessed using immunohistochemistry in OS tissues (n=152), osteochondroma tissues (n=91), and normal bone tissues (n=74). We carried out a follow-up lasting 8-60 months to investigate HBME-1 expression and its correlations with the clinical pathological characteristics and prognosis of OS. RESULTS HBME-1 was highly expressed in OS tissues compared with osteochondroma tissues and normal bone tissues, and was highly expressed in osteochondroma tissues compared with normal bone tissues (all P<0.05). HBME-1 expression was correlated with clinical stages, postoperative recurrence, metastasis, and 5-year survival (all P<0.05). The area under the receiver operating characteristics curve of HBME-1 expression was 0.864, with sensitivity of 80.92%, specificity of 91.89%, and accuracy of 84.51%. The survival rate was lower in the HBME-1 positive expression group than the HBME-1 negative expression group (P<0.05). Clinical stages, metastasis, and HBME-1 expression were independent risk factors for the survival of patients with OS (all P<0.05). CONCLUSIONS HBME-1 expression was correlated with the occurrence and development of OS. HBME-1 positive expression was a risk factor for the prognosis of OS.


Assuntos
Biomarcadores Tumorais/biossíntese , Neoplasias Ósseas/metabolismo , Neoplasias Ósseas/patologia , Osteossarcoma/metabolismo , Osteossarcoma/patologia , Adolescente , Adulto , Criança , Transição Epitelial-Mesenquimal , Feminino , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Osteocondroma/metabolismo , Osteocondroma/patologia , Prognóstico , Curva ROC , Fatores de Risco
8.
Mitochondrial DNA A DNA Mapp Seq Anal ; 27(3): 1688-92, 2016 05.
Artigo em Inglês | MEDLINE | ID: mdl-25211088

RESUMO

Pseudanoplocephala crawfordi is one of the important zoonotic cestodes causing economic significance and public health concern. In the present study, the phylogenetic position of P. crawfordi isolated from pigs was re-inferred using molecular markers of internal transcribed spacer ribosomal DNA (ITS rDNA) and partial NADH dehydrogenase subunit 1 (pnad1) mitochondrial DNA. The lengths of ITS1, ITS2 rDNA and pnad1 were 757 bp, 628 bp and 458 bp, respectively. Sequence differences in the ITS1, ITS2 rDNA and pnad1 between P. crawfordi and Hymenolepis species were smaller than that between cestodes within genus Hymenolepis. Phylogenetic analyses based on three gene fragments showed that P. crawfordi was grouped into cluster of Hymenolepis species. These results suggested that P. crawfordi would be one member of genus Hymenolepis but not in a new genus Pseudanoplocephala.


Assuntos
DNA Mitocondrial/genética , DNA Ribossômico/genética , Hymenolepis/classificação , Hymenolepis/genética , Filogenia , Animais , Sequência de Bases , Geografia , Suínos/parasitologia
9.
Int J Clin Exp Med ; 8(7): 11818-23, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26380023

RESUMO

Increasing studies have demonstrated that altered expression of histone deacetylases (HDACs) plays a critical role in the tumorigenesis through up-regulation or down-regulation of key genes involved in cell proliferation, cell-cycle regulation and apoptosis. In the present study, the expression and function of HDAC9 were investigated in osteosarcoma. Quantitative real-time PCR and Western blot analysis found that HDAC9 was up-regulated in osteosarcoma tissues, when compared with that in adjacent normal tissues. In vitro studies further demonstrated that overexpression of HDAC9 in U2OS and MG63 cells promoted cell proliferation and invasion. Using chromatin immunoprecipitation (ChIP) assay, we found that HDAC9 epigenetically repressed p53 transcription through binding to its proximal promoter region. Therefore, our data suggest an important role for HDAC9/p53 regulatory pathway in the osteosarcoma progression.

10.
Ying Yong Sheng Tai Xue Bao ; 26(4): 1223-30, 2015 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-26259467

RESUMO

A field study was conducted to investigate the influences of no-tillage, stubble retention and crop type on weed density, species composition and community feature in a rotation system (winter wheat-common vetch-maize) established 12 years ago on the Loess Plateau of eastern Gansu. This study showed that the weed species composition, density and community feature varied with the change of crop phases. No-tillage practice increased the weed density at maize phase, while rotation with common vetch decreased the density in the no-tillage field. Stubble retention reduced the weed density under maize phase and the lowest density was observed in the no-tillage plus stubble retention field. No-tillage practice significantly increased the weed species diversity under winter wheat phase and decreased the diversity under common vetch phase. At maize phase, a greater species diversity index was observed in the no-tillage field. These results suggested that no-tillage practice and stubble retention possibly suppress specific weeds with the presence of some crops and crop rotation is a vital way to controlling weeds in a farming system.


Assuntos
Agricultura/métodos , Plantas Daninhas/crescimento & desenvolvimento , Biodiversidade , China , Produtos Agrícolas , Estações do Ano , Triticum , Vicia , Zea mays
11.
Med Sci Monit ; 21: 32-5, 2015 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-25556778

RESUMO

BACKGROUND: The aim of this study was to design a self-retaining rat contact lens to simplify intravitreous injection in rats. MATERIAL/METHODS: A self-retaining, plane-concave prism contact lens customized for rats was designed. Forty diabetic rats were randomly divided into 2 groups and received the intravitreous injection of 10 µl of a cell suspension containing bone marrow-derived stroma cell (BMSC). Group A: used a microsyringe and a rat contact lens (n=20). Group B: used the same microsyringe and a traditional cover-slip (n=20).The duration of the intravitreous injection course and the success rate of intravitreous injection were observed. RESULTS: With the use of a self-retaining rat contact lens, a clear and stable view of the rat fundus was provided and the intravitreous injection course of rats quickly achieved, averaging 4.65±0.53 min in Group A and 12.33±2.79 min in Group B. The difference was statistically significant, and the time saved averaged 7.68 min. None of the Group A rats had retinal bleeding or lens injury; whereas 2 of 20 Group B rats had bleeding and 1 of 20 had lens injury. There was no significant difference between the rats in Group A and Group B. CONCLUSIONS: A self-retaining rat contact lens is a potentially powerful instrument that allows high-quality observation of the rat fundus and simplifies the course of intravitreal injection.


Assuntos
Lentes de Contato , Injeções Intravítreas/métodos , Animais , Córnea/fisiologia , Elasticidade , Desenho de Equipamento , Fundo de Olho , Hemorragia , Masculino , Ratos , Ratos Sprague-Dawley , Seringas , Viscosidade
12.
Zhongguo Zhong Yao Za Zhi ; 32(20): 2107-9, 2007 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-18306738

RESUMO

OBJECTIVE: To analyze the inoculation ratio and echinacoside content of Cistanche tubulosa and provide theoretical basis for Tamarix introduction, resource protection and screening of C. tubulosa. METHOD: 8 Tamarix species were introduced in the North China Plain and inoculation of C. tubulosa was conducted on all species. Phenylethanoid glycosides fingerprinting and echinacoside content of C. tubulosa were analyzed by using HPLC. RESULT: The adaptability of 8 Tamarix species were significantly different, phenylethanoid glycosides component of C. tubulosa on T. gansuensis and T. austromongolica were basically identical in contrast to T. chinensis, echinacoside content showed no obvious difference in C. tubulosa plant growing 4 months. CONCLUSION: T. gansuensis and T. Austromongolica are suitable for the host introduction plant of C. tubulosa resource protection and screening in North China Plain.


Assuntos
Cistanche/crescimento & desenvolvimento , Glicosídeos/análise , Fenóis/análise , Tamaricaceae/crescimento & desenvolvimento , China , Cistanche/química , Conservação dos Recursos Naturais , Ecossistema , Plantas Medicinais/química , Plantas Medicinais/crescimento & desenvolvimento , Chuva , Solo , Tamaricaceae/classificação
13.
Zhongguo Zhong Yao Za Zhi ; 29(11): 1057-60, 2004 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-15656138

RESUMO

OBJECTIVE: To compare and analyze the organic acids level in fruit of Crataegus pinnatifida var. major before and after processing. METHOD: The amount of total organic acids and individual citric acid, malate acid, ursolic acid and oleanolic acid in fruit of C. pinnatifida var. major before and after processing were determined by pH titration and HPLC analysis. RESULT: Good linear relationship between peakarea and amount was noted for 0.28-9.02 microg of malate acid, with a correlation coefficient of 0.9998. Regression equation was Y = 2344.7 + 49309.6 X. Good linear relationships between the logarithm of peak area and amount were noted for 0.348-1.74 microg of usolic acid and 0.498-2.48 microg of oleanolic acid, with a correlation coefficient of 0.9998 in each case. Regression equations were Y = 1.3628 X + 5.9508 and Y = 1.4447 X + 5.8236, respectively. CONCLUSION: The lipid-soluble organic acids mostly remain whereas only about 70% of the water-soluble acids remain after processing in fruit of C. pinnatifida var. major.


Assuntos
Crataegus/química , Malatos/análise , Ácido Oleanólico/análise , Plantas Medicinais/química , Triterpenos/análise , Ácido Cítrico/análise , Medicamentos de Ervas Chinesas/química , Frutas/química , Temperatura Alta , Tecnologia Farmacêutica
14.
Zhongguo Zhong Yao Za Zhi ; 28(10): 913-7, 2003 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-15620177

RESUMO

OBJECTIVE: To review the application of Evaporative Light-Scattering Detector in analysis of the active constituents in Chinese herbal medicine. METHOD: Papers on the assay of the active compounds of Chinese herbal medicine by HPLC-ELSD method were collected. RESULT: Evaporative Light-Scattering Detector has been widely used in assaying the compounds which have no UV absorption or when wavelengths for maximal UV absorption are under 200 nm in natural products. CONCLUSION: Evaporative Light-Scattering Detector will have a more widely application in analysis of constituents in Chinese herbal medicine.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Medicamentos de Ervas Chinesas/isolamento & purificação , Plantas Medicinais/química , Medicamentos de Ervas Chinesas/química , Controle de Qualidade , Espalhamento de Radiação , Luz Solar
15.
Zhongguo Zhong Yao Za Zhi ; 27(11): 808-11, 2002 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-12776581

RESUMO

OBJECTIVE: To review the application of the pattern recognition to quality assessment of the Chinese Traditional Medicine. METHOD: Papers of application of chemical pattern recognition, pattern recognition of stereoscopic features and artificial neural networks to quality assessment of the Chinese Traditional Medicine were collected and the application of different method was reviewed. RESULT: IR, UV, GC, GC-Mass and the sum mass spectra were used to evaluate the chemical character of different samples, and the microscopic features of the epidermis were scored quantitatively by stereology and image analysis on the basis of classical taxonomy,. BP networks were also widely used. CONCLUSION: Chemical pattern recognition, pattern recognition of stereoscopic features and artificial neural networks are of important use in quality assessment of material medica.


Assuntos
Reconhecimento Automatizado de Padrão , Plantas Medicinais , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/isolamento & purificação , Medicamentos de Ervas Chinesas/normas , Redes Neurais de Computação , Farmacognosia , Plantas Medicinais/anatomia & histologia , Plantas Medicinais/química , Controle de Qualidade
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