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1.
Gene ; 807: 145949, 2022 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-34481004

RESUMO

Growth traits is a critical economic trait for animal husbandry. In this study, the SNPs of CTNNA3 and CAP2 genes were investigated to check whether they are associated with growth traits (body weight, body height, body length and chest circumference) in Hu sheep. The result of the association analysis indicated that the mutation in CTNNA3 (g.2018018 A > G) were associated significantly with body weight, body height, body length and chest circumference (P < 0.05), the mutation in CAP2 (g.8588 T > C) were associated significantly with body height at 140, 160, 180 days (P < 0.05), AA and CC of CTNNA3 and CAP2 were the dominant genotypes associated with growth traits in Hu sheep. Moreover, combined effect analyses indicated that the growth traits with combined genotypes AACTNNA3-CCCAP2 and AACTNNA3-CTCAP2 were higher than those with genotype GGCTNNA3-CTCAP2. RT-qPCR indicated that CTNNA3 expression levels were significantly higher in liver and lung than in other nine tissues (P < 0.05), CAP2 expression levels were significantly higher in bone, heart, liver, lung and duodenum than in other six tissues (P < 0.05). In conclusion, CTNNA3 and CAP2 polymorphisms could be used as genetic markers for improving growth traits in Hu sheep husbandry.

2.
Gene ; 807: 145954, 2022 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-34500050

RESUMO

Reducing tail fat deposition can increase the economic value of a carcass and improve feed efficiency. This study aimed to explore ELOVL5 and FASN polymorphisms associated with tail fat deposition and their expression levels of sheep. Association analysis showed that ELOVL5 synonymous mutation g.62534 C > T was associated with tail width, tail fat weight, and relative tail fat weight (P < 0.05). FASN synonymous mutation g.12694 A > G was associated with tail length and width (P < 0.05). Combined effect analyses indicated significant differences between the combined genotypes and tail fat deposition. Quantitative real-time reverse transcription PCR indicated that the ELOVL5 and FASN expression levels were significantly higher in tail fat than in other tissues (P < 0.05). ELOVL5 expression levels in tail-fat tissue of big-tail sheep was significantly higher than that in small-tail sheep (P < 0.01). FASN expression levels were significantly higher in tail-fat tissue of small-tail sheep than in that of big-tail sheep (P < 0.05). During development, ELOVL5 tail fat expression increased significantly from 0 to 6 months old (P < 0.05), and FASN expression at 3 months old was significantly higher than that at 0 (minimum) and 6 months old (P < 0.05). Therefore, ELOVL5 and FASN polymorphisms could represent new candidate molecular markers and targets to reduce tail fat deposition in sheep.

3.
Bioact Mater ; 7: 144-153, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34466723

RESUMO

Magnesium alloys with integration of degradability and good mechanical performance are desired for vascular stent application. Drug-eluting coatings may optimize the corrosion profiles of magnesium substrate and reduce the incidence of restenosis simultaneously. In this paper, poly (trimethylene carbonate) (PTMC) with different molecular weight (50,000 g/mol named as PTMC5 and 350,000 g/mol named as PTMC35) was applied as drug-eluting coatings on magnesium alloys. A conventional antiproliferative drug, paclitaxel (PTX), was incorporated in the PTMC coating. The adhesive strength, corrosion behavior, drug release and biocompatibility were investigated. Compared with the PLGA control group, PTMC coating was uniform and gradually degraded from surface to inside, which could provide long-term protection for the magnesium substrate. PTMC35 coated samples exhibited much slower corrosion rate 0.05 µA/cm2 in comparison with 0.11 µA/cm2 and 0.13 µA/cm2 for PLGA and PTMC5 coated counterparts. In addition, PTMC35 coating showed more stable and sustained drug release ability and effectively inhibited the proliferation of human umbilical vein vascular smooth muscle cells. Hemocompatibility test indicated that few platelets were adhered on PTMC5 and PTMC35 coatings. PTMC35 coating, exhibiting surface erosion behavior, stable drug release and good biocompatibility, could be a good candidate as a drug-eluting coating for magnesium-based stent.

4.
Appl Opt ; 60(21): 6030-6043, 2021 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-34613267

RESUMO

White light interferometry is a well-established surface recovery technique. In this paper, a white light signal processing algorithm based on phase error compensation using spectrum selection is proposed. The derived nonlinear phase distribution from the correlogram is modeled as the combination of random errors and systemic deviations. By developing a new, to the best of our knowledge, recovery algorithm, the phase noise can be separated from the linear map and significantly attenuated. Based on the proposed algorithm, the spectrum features of white light LEDs and halogen lamps are investigated in detail. The inner products defined by three selected points are employed to generate a coefficient to evaluate the linearity of an unwrapped phase map within a certain spectrum region. The optimal spectrum range corresponding to the best measurement performance can then be located where the coefficient approximates 1 and the spectrum energy stays relatively high. The simulations are carried out under different levels of SNR and scan step noises, which show that the new method can effectively reduce additional disturbance from the recovered topography. In experiments, the system with the proposed method is first calibrated by a step height standard (VLSI, 182.7±2.0nm) with the repeatability of 0.44 nm. A silicon wafer and three roughness standards are also tested to further verify the robustness of the new method.

5.
Materials (Basel) ; 14(19)2021 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-34640172

RESUMO

Mechanical joints are commonly required in structures made of fiber metal laminate (FML), which pose a threat due to multi-site stress concentrations at rivet or bolt holes. Thus, for a reasonably designed FML joint, it is essential to characterize the failure mechanism of multiple-hole FML; however, little information about this has been found in open literature. In the present work, influences of hole arrangement and loading strategy (on-axis or off-axis) on the failure mechanism of multiple-hole FML were investigated, by performing finite element analyses and energy dissipation analyses with elastoplastic progressive damage models that took curing stress into account. Six types of specimens with holes arranged in parallel and staggered forms were designed, whose geometrical parameters were in strict accordance with those specified for composites joints. It indicated that the stress distribution, gross/net notched strength, critical fracture path, and damage evaluation process were only slightly influenced by the hole number and hole arrangement. On the other hand, they were strongly influenced by the loading strategy, due to the transition of failure domination. Results presented here can provide evidence for introducing design regulations of composite joints into the more hybrid FML, and for reasonably determining its multiple-hole strength merely based on the sing-hole specimen.

6.
Analyst ; 2021 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-34643194

RESUMO

Solid-state nanochannels have attracted considerable attention for their similar ion transport properties to biological ion channels. The construction of porous ion channels with good stability at the submicro/micrometer scale is very beneficial to develop large-area ion channel devices. In this manuscript, based on in-situ thermal crosslinking of a small organic molecule containing triphenylamine and styrene groups, we construct a heterogeneous membrane with asymmetrical charge and wettability on cylindrical anodic aluminum oxide (AAO) channels (D ≈ 319 nm). This heterogeneous membrane has typical ion current rectification characteristics with a high rectification ratio of 36.9 and good stability. This work provides an effective strategy for the construction of submicrochannel heterogeneous membranes and also broadens the application range of bionic ion channels.

7.
Environ Pollut ; 292(Pt A): 118317, 2021 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-34634407

RESUMO

Neonicotinoids have been often detected in aquatic environment with high concentrations; however, little is known about their risk and fate to/in fish. This study systematically investigated the bio-uptake, tissue distribution and metabolism of neonicotinoids in zebrafish, taking clothianidin (CLO) as an example. The results revealed the uptake and elimination kinetics of CLO in whole fish and different tissues was very similar, and its bioconcentration factor (<1) indicates the low bioaccumulation potential in zebrafish. The highest accumulative tissues for CLO were found to be intestine and liver. Eight biotransformation products were identified in intestine and liver, and the metabolic pathways were found to be N-demethylation and nitro-reduction. The metabolic kinetics of two products (desmethyl clothianidin and clothianidin urea) revealed the metabolism of CLO mainly occurred in liver and intestine. This suggested that the hepatobiliary system played an important role in the metabolism and elimination of CLO. This study provides a comprehensive evaluation of the toxicokinetics of CLO in zebrafish, and these results can contribute to its ecological risk assessment.

8.
Mol Biol Rep ; 2021 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-34623593

RESUMO

Methamphetamine is a highly addictive central stimulant with extensive and strong neurotoxicity. The neurotoxicity of methamphetamine is closely related to the imbalance of dopamine levels and the destruction of the blood-brain barrier. An increase in dopamine may induce adverse effects such as behavioral sensitization and excessive locomotion. Damage to the blood-brain barrier can cause toxic or harmful substances to leak to the central nervous system, leading to neurotoxicity. The renin-angiotensin system is essential for the regulation of dopamine levels in the brain. Matrix metalloproteinase-9 causes reward effects and behavioral sensitization by inducing dopamine release. Prolactin has been shown to be involved in the regulation of tight junction proteins and the integrity of the blood-brain barrier. At present, the treatment of methamphetamine detoxification is still based on psychotherapy, and there is no specific medicine. With the rapid increase in global seizures of methamphetamine, the treatment of its toxicity has attracted more and more attention. This review intends to summarize the therapeutic mechanisms of renin-angiotensin inhibitors, matrix metalloproteinase-9 inhibitors and protein hormones (prolactin) on methamphetamine neurotoxicity. The repair effects of these three on methamphetamine may be related to the maintenance of brain dopamine balance and the integrity of the blood-brain barrier. This review is expected to provide the new therapeutic strategy of methamphetamine toxicity.

9.
Int J Immunopathol Pharmacol ; 35: 20587384211048265, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34608813

RESUMO

INTRODUCTION: Increasing evidence indicates that lncRNA TUG1 represents an oncogenic factor in cancer. But, the mechanisms by which lncRNA TUG1 contributes to lung adenocarcinoma (LAC) remain undocumented. METHODS: The relationship between lncRNA TUG1/miR-138-5p expression and clinical outcomes in patients with LAC was indicated by qPCR, FISH, and TCGA cohort. Gain- or loss-of-function experiments and in vivo tumorigenesis were used to assess the role of lncRNA TUG1 in LAC. The interplay between TUG1 and miR-138-5p was validated by luciferase gene report and RIP assays. qPCR and Western blot analyses were used to investigate the effects of TUG1 on miR-138-5p/HIF1A axis in LAC cells. RESULTS: We found that upregulation of TUG1 or downregulation of miR-138-5p was associated with lymph node or distant metastasis and indicated a poor survival in LAC. Reduced expression of TUG1 restrained the growth of LAC cells, while restored expression of TUG1 had the opposite effects. TUG1 was identified to negatively regulate miR-138-5p expression, and miR-138-5p reversed TUG1-induced cell proliferation by targeting HIF1A. Elevated expression of HIF1A predicted a poor survival in LAC. CONCLUSION: Our findings demonstrate that lncRNA TUG1 promotes the growth of LAC by regulating miR-138-5p-HIF1A axis.

10.
Opt Lett ; 46(19): 4805-4808, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34598204

RESUMO

A novel, to the best of our knowledge, tiny velocity measurement system is proposed and demonstrated. This proposed system employs an interference structure in which the reference and measurement paths are filled by two light beams carrying opposite-sign orbital angular momentum (OAM), respectively. The tiny velocity to be measured in the measurement path causes the change of the light path and results in a time-varying phase shift between the reference and measurement paths. This time-varying phase shift leads to the rotation of the petal-like light spot obtained by the interference between two paths. The rotating angular velocity of the petal-like light spot is proportional to the time-varying phase shift caused by the tiny velocity, and it is measured by a chopper and a single-point detector instead of array detectors. This proposed system has a simple structure and achieves a high-accuracy tiny velocity measurement with a measurement error rate that is less than 10 nm/s.

11.
Emerg Microbes Infect ; : 1-9, 2021 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-34666622

RESUMO

Neutralizing antibodies waned significantly six months after two-dose procedure of inactivated SARS-CoV-2 vaccine. However, it is not clear whether a third booster dose could quickly activate the body's immune response against the emerging SARS-CoV-2 variants. This study demonstrated that after a third booster dose of inactivated vaccine, immune memory can be quickly awakened, and the antibody response recovered after seven days. Importantly, although the titers decreased, the neutralizing antibodies at 14 days after the third dose can still neutralize alpha, beta, and delta variants.

12.
J Phys Chem Lett ; 12(39): 9579-9583, 2021 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-34582204

RESUMO

Oxygen vacancy is a common defect in metal oxides that causes appreciable damage to material properties and performance. Removing bulk defects of oxygen vacancy (VO) typically needs harsh conditions such as high-temperature annealing. Supported by first-principles simulations, we propose an effective strategy of removing VO bulk defects in metal oxides by evaporating hydrogen dopants. The hydrogen dopants not only lower the migration barrier of VO but also push VO away due to their repulsive interaction. The coevaporation mechanism was supported by a neural networks potential-based molecular dynamics simulation, which shows that the migration of hydrogen dopants from inside to surface at 400 K promotes the migration of VO as well. Our proof-of-concept study suggests an alternative and efficient way of modulating oxygen vacancies in metal oxides via reversible hydrogen doping.

13.
Int J Mol Sci ; 22(18)2021 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-34575987

RESUMO

Food-derived oligosaccharides show promising therapeutic potential in lowering blood pressure (BP), but the mechanism is poorly understood. Recently, the potential role of gut microbiota (GM) in hypertension has been investigated, but the specific GM signature that may participate in hypertension remains unclear. To test the potassium alginate oligosaccharides (PAO) mechanism in lowering BP and specific microbial signature changes in altering GM, we administered various dosages of PAO in 40 spontaneously hypertensive rats for a duration of six weeks. We analyzed BP, sequenced the 16S ribosomal DNA gene in the cecum content, and gathered RNA-seq data in cardiac tissues. We showed that the oral administration of PAO could significantly decrease systolic BP and mean arterial pressure. Transcriptome analyses demonstrated that the protective effects of developing heart failure were accompanied by down-regulating of the Natriuretic Peptide A gene expression and by decreasing the concentrations of angiotensin II and atrial natriuretic peptide in plasma. In comparison to the Vehicle control, PAO could increase the microbial diversity by altering the composition of GM. PAO could also decrease the ratio of Firmicutes to Bacteroidetes by decreasing the abundance of Prevotella and Phascolarctobacterium bacteria. The favorable effect of PAO may be added to the positive influence of the abundance of major metabolites produced by Gram-negative bacteria in GM. We suggest that PAO caused changes in GM, and thus, they played an important role in preventing the development of cardiovascular disease.

14.
Nanomaterials (Basel) ; 11(9)2021 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-34578475

RESUMO

Interfacial interaction is one of the most important factors in the construction of high-performance graphene-based elastomer composites. In this paper, graphene/poly (styrene-b-isoprene-b-styrene) (SIS) composites were prepared with solution mixing followed by an evaporation-induced self-assembly process. Various techniques such as scanning electron microscopy, UV-vis absorption spectra, tensile testing, Shore A hardness, surface resistance, thermal conductivity, and thermogravimetric analysis were conducted to characterize the microstructure and properties of the obtained composites. The results showed that the π-π stacking interfacial interaction between phenyl groups of SIS and graphene play an important role in the properties' improvement, and the effect of interfacial interaction on the properties was revealed.

15.
Chemosphere ; 287(Pt 3): 132303, 2021 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-34562705

RESUMO

Neonicotinoids are widely used pesticides all over the world and pose severe water pollution. Although they can be degraded via absorbing sunlight, few attentions have been paid to the environmental risks of their photolysis products. In this paper, the photo-toxicity was investigated for four neonicotinoids (dinotefuran, nitenpyram, thiamethoxam and clothianidin) based on a series of experiments (i.e., photolysis kinetics, radical scavenging, bioluminescent inhibition test to Vibrio Fischeri and intermediate identification) and in-silico calculation of photolysis pathway. The results show that direct photolysis dominates the photolysis of the four neonicotinoids under simulated sunlight radiation. The bioluminescent inhibition kinetics shows that all four neonicotinoids have photo-induced toxicity to V. fischeri, but with different light-induced responses. Scavenging radicals (·OH and 1O2) will decrease the photo-induced toxicity of all the four neonicotinoids, indicating radicals play important roles to the photo-chemical reactions of intermediates. Dissolved organic matters exhibit slightly shading effect to the photolysis rates of four parent compounds. However, the ROSs generated by DOM can accelerate the photo-chemical reactions of intermediates, leading to different photo-induced toxicity in present of DOM. According to the detected intermediates and Gaussian calculations, there are different photolysis pathways and mechanisms for the four neonicotinoids. The calculation for photo-sensitization reactions with 3O2 indicates that both energy transfer reactions and electron transfer reactions can be produced under simulated sunlight radiation, which further consolidate that reactive oxygen species are involved in the photolysis process. A theoretical model has been developed to explain the toxicity variations of four neonicotinoids in different aqueous conditions.

16.
Transl Pediatr ; 10(8): 2131-2135, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34584884

RESUMO

Agnathia-otocephaly complex (AOC) is a rare and complex craniofacial malformation characterized by mandibular hypoplasia or agnathia, auricular fusion (synotia), and microstomia with oroglossal hypoplasia or aglossia. It can occur alone or in combination with forebrain anomalies and cardiac malformations and has an extremely poor prognosis. Here, we report a case of AOC diagnosed by systemic fetal screening at a gestational age of 25+4 weeks. Ultrasound revealed that the S-curve formed by the normal lower jaw and lower lip had disappeared, the lower jaw and mandible were invisible, the mouth was extremely small, and the oral fissure was "pinhole-shaped". There was a cone-shaped perioral bulge. Both ears were located in the front side of the neck, and the right foot was inverted. Excessive amniotic fluid was observed. The absence of a mandible was confirmed on X-ray examination after induced abortion. Specimen observation showed that the ear positions were extremely low, and both earlobes were connected in the front side of the neck. It was particularly challenging to identify the development of the mandible and locate auricles during prenatal ultrasound diagnosis, and the prenatal diagnosis of AOC was confirmed by combining two-dimensional and three-dimensional ultrasound in our current case.

17.
Artigo em Inglês | MEDLINE | ID: mdl-34562085

RESUMO

CONTEXT: The hemoglobin glycation index(HGI) is correlated with metabolic diseases and inflammations. Whether the HGI is associated with the ageing process and how inflammation and oxidative stress affect the relationship remain unclear. OBJECTIVE: We aim to analyze links between HGI and ageing biomarkers, and to explore a potential role of inflammation and oxidative stress in the correlations. METHODS: A cross-sectional study of 434 subjects with different glucose intolerances in a rural community was enrolled. The HGI was calculated as the difference between the measured and predicted hemoglobin A1c(HbA1c). The population was categorized into tertiles of HGI. Telomere length(LTL) and mitochondrial DNA copy number(mtDNAcn) determined by PCR assay. Tumor necrosis factor α(TNFα) and interleukin 6(IL-6), 8-oxo-2'-deoxyguanosine(8-oxo-dG), superoxide dismutase(SOD) activities and glutathione reductase(GR) were measured. RESULT: Participants in the high HGI group were older and reported a shorter LTL, higher levels of TNFα, SOD activities and HbA1c. Correlation analyses demonstrated that HGI was correlated with LTL(r=-0.25,p<0.001) and TNFα(r=0.19,p<0.001) regardless of HbA1c levels. No relationship was found between HGI and mtDNAcn. HGI(ß=-0.238,95%CI(-0.430,-0.046),p=0.015) and TNFα(ß=-0.02,95%CI(-0.030,-0.014),p<0.001) were proved to be correlated with LTL independently using multiple linear regression analysis. Ordinal logistic regression models showed that compared with subjects in High-HGI, the possibilities of a higher-level LTL was 5.29-fold in Low-HGI(OR5.29,95%CI(2.45,11.41),p<0.001), 2.41-fold in Moderate-HGI (OR2.41,95%CI(1.35,4.30),p=0.003) after controlling for confounding variables. Mediation analyses indicated that TNFα accounted for 30.39% effects of HGI on LTL. CONCLUSION: HGI was negatively related to telomere attrition, independent of HbA1c. TNFα acted as a mediator of the relationship between HGI and LTL.

18.
J Hazard Mater ; 423(Pt A): 127110, 2021 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-34523489

RESUMO

Cadmium (Cd) is a known nephrotoxic heavy metal and proximal tubules are the major target of Cd-induced acute kidney injury (AKI). We previously demonstrated that lysosomal dysfunction and dysregulated autophagy contribute to Cd-induced AKI. Recent studies have revealed that bromodomain-containing protein 4 (BRD4) is a transcriptional repressor of autophagy and lysosomal function. Hence, in vivo and in vitro studies were performed to clarify the role of BRD4 in Cd-induced AKI. Firstly, Cd has no effect on BRD4 expression levels, but increases H4K16 acetylation. Resultantly, Cd promotes the recruitment of BRD4 to lysosomal gene promoter regions to make it as a transcriptional regulator. Pharmacological and genetic inhibition of BRD4 alleviates Cd-inhibited lysosomal gene transcript levels and lysosomal function, leading to the alleviation of Cd-induced autophagy inhibition. Moreover, inhibition of BRD4 relieves Cd-induced oxidative stress and concurrent cytotoxicity, which is counteracted by the inhibition of autophagy via Atg5 knockdown, indicating that alleviation of oxidative stress by BRD4 inhibition is ascribed to its restoration of autophagic flux. Collectively, these results demonstrate that BRD4 acts as a transcriptional repressor to mediate lysosomal dysfunction, autophagy blockade and oxidative stress during Cd exposure, which may be a potential therapeutic target for Cd-induced AKI.

19.
Neoplasma ; 2021 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-34533029

RESUMO

The crosstalk between tumor and stroma plays a critical role in cancer metastasis. However, the function of miR-10a-5p on liver fibroblasts in the metastatic microenvironment of colon cancer (CC) and the effect of activated fibroblasts on CC cells are still unclear. In our study, miR-10a-5p overexpression inhibited the proliferation, migration, and IL-6/IL-8 level of LX-2 and human liver cancer fibroblasts (HLCFs) cells. Moreover, miR-10a-5p had lower expression in HLCFs than in human liver normal fibroblasts (HLNFs). The conditioned medium (CM) from LX-2 cells with miR-10a-5p overexpression and HLNFs could inhibit the invasion, migration, and stemness of CC SW480 cells, whereas HLCFs CM could promote these malignant phenotypes of SW480 cells. The present study illustrates the effect of miR-10a-5p on the liver fibroblasts and the altered liver fibroblasts in the microenvironment on CC cells induced by miR-10a-5p, which may aid the understanding of the mechanisms underlying CC liver metastasis.

20.
Cell Regen ; 10(1): 30, 2021 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-34487238

RESUMO

Polycomb repressive complexes (PRCs) are essential in mouse gastrulation and specify neural ectoderm in human embryonic stem cells (hESCs), but the underlying molecular basis remains unclear. Here in this study, by employing an array of different approaches, such as gene knock-out, RNA-seq, ChIP-seq, et al., we uncover that EZH2, an important PRC factor, specifies the normal neural fate decision through repressing the competing meso/endoderm program. EZH2-/- hESCs show an aberrant re-activation of meso/endoderm genes during neural induction. At the molecular level, EZH2 represses meso/endoderm genes while SOX2 activates the neural genes to coordinately specify the normal neural fate. Moreover, EZH2 also supports the proliferation of human neural progenitor cells (NPCs) through repressing the aberrant expression of meso/endoderm program during culture. Together, our findings uncover the coordination of epigenetic regulators such as EZH2 and lineage factors like SOX2 in normal neural fate decision.

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