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1.
Artigo em Inglês | MEDLINE | ID: mdl-32096300

RESUMO

A divergent synthetic approach to six Ganoderma meroterpenoids, namely ganocins A-C, ganocochlearins C-D, and cochlearol T, has been developed for the first time. This concise synthetic route features a two-phase strategy which includes early-stage rapid construction of planar common tricyclic intermediate followed by late-stage highly selective transformations into various Ganoderma meroterpenoids. Key to the success of the strategy are a bio-inspired intramolecular hetero-Diels-Alder reaction and Stahl-type oxidative aromatization, which allowed us to efficiently forge the common tricyclic phenol intermediate. A nucleophilic dearomatization of the phenol unit, combined with a regioselective 1,4-reduction of the resulting dienone, then enabled rapid access to the framework of ganocins B-C. Additionally, site-selective Mukaiyama hydration, followed by an intramolecular oxa -Michael addition/triflation cascade, served as a key strategic element in the chemical synthesis of ganocin A.

2.
Artigo em Inglês | MEDLINE | ID: mdl-31957609

RESUMO

Antimicrobial peptides (AMPs) are natural polypeptides with antimicrobial activities and are found in most organisms. AMPs are evolutionarily conservative components that belong to the innate immune system and show potent activity against bacteria, fungi, viruses and in some cases display antitumor activity. Thus, AMPs are major candidates in the development of new antibacterial reagents. In the last few decades, AMPs have attracted significant attention from the research community. During the early stages of the development of this research field, AMPs were experimentally identified, which is an expensive and time-consuming procedure. Therefore, research and development (R&D) of fast, highly efficient computational tools for predicting AMPs has enabled the rapid identification and analysis of new AMPs from a wide range of organisms. Moreover, these computational tools have allowed researchers to better understand the activities of AMPs, which has promoted R&D of antibacterial drugs. In this review, we systematically summarize AMP prediction tools and their corresponding algorithms used.

3.
Braz Oral Res ; 33: e117, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31939498

RESUMO

The aim of this study was to evaluate the effect of mineral trioxide aggregate (MTA) and Brazilian propolis on the cell viability, mineralization, anti-inflammatory ability, and migration of human dental pulp cells (hDPCs). The cell viability was evaluated with CCK-8 kit after 1, 5, 7, and 9 days. The deposition of calcified matrix and the expression of osteogenesis-related genes were evaluated by Alizarin Red staining and real-time PCR after incubation in osteogenic medium for 21 days. The expression of inflammation-related genes in cells was determined after exposure to 1 µg/mL LPS for 3 h. Finally, the numbers of cells that migrated through the permeable membranes were compared during 15 h. Propolis and MTA significantly increased the viability of hDPCscompared to the control group on days 7 and 9. In the propolis group, significant enhancement of osteogenic potential and suppressed expression of IL-1ß and IL-6 was observed after LPS exposure compared to the MTA and control groups. The number of migration cells in the propolis group was similar to that of the control group, while MTA significantly promoted cell migration. Propolis showed comparable cell viability to that of MTA and exhibited significantly higher anti-inflammatory and mineralization promotion effects on hDPCs.


Assuntos
Compostos de Alumínio/farmacologia , Anti-Inflamatórios/farmacologia , Compostos de Cálcio/farmacologia , Polpa Dentária/citologia , Polpa Dentária/efeitos dos fármacos , Óxidos/farmacologia , Própole/farmacologia , Silicatos/farmacologia , Antraquinonas , Brasil , Movimento Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Combinação de Medicamentos , Humanos , Interleucina-1beta/análise , Interleucina-6/análise , Odontoblastos/efeitos dos fármacos , Reação em Cadeia da Polimerase em Tempo Real , Reprodutibilidade dos Testes , Estatísticas não Paramétricas , Fator de Necrose Tumoral alfa/análise
4.
Org Lett ; 21(23): 9306-9310, 2019 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-31668081

RESUMO

A new class of π-conjugated organic fluorophores containing a 6H-phenanthro[4,5-cde]pyreno[4',5':4,5]imidazo[1,2-a]azepin-6-one core was synthesized by a facile one-pot condensation method. The molecular structures and solid-state packing properties of these compounds were investigated by X-ray single crystallographic analysis. UV-vis absorption and fluorescence spectroscopic studies disclosed interesting mechanofluorochromic properties in the solid state and highly sensitive acidochromic behavior in the solution phase.

5.
Front Physiol ; 10: 1304, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31695620

RESUMO

Pulpitis in primary teeth, a condition caused by presence of bacteria, is highly prevalent worldwide. The use of biocompatibility materials with anti-inflammatory, anti-bacterial, and regenerative properties is critical for prognosis of this endodontic disease. This study aimed to identify expression of human ß defensin 4 (HBD4) in stem cells derived from human exfoliated deciduous teeth (SHED) and characterize the effects of HBD4 on SHED. Quantitative polymerase chain reaction (qPCR) was used to detect HBD4 expression in SHED and the effect of HBD4 on inflammatory factors in lipopolysaccharide (LPS)-stimulated SHED. Affinity measurement was made by the Fortebio Octet System to explore the potential interaction between LPS and HBD4. Western blot analysis was used to explore the effect of HBD4 on mitogen-activated protein kinase (MAPK) pathway. Colony-forming unit methods and scanning electron microscopy were applied to study antimicrobial effect of HBD4 on Fusobacterium nucleatum and Porphyromonas gingivalis. Alkaline phosphatase staining, alizarin red staining, qPCR and western blot were taken to detect effects of HBD4 on osteoblast/odontoblast differentiation of SHED. RT2 Profiler PCR Array was used to explore the potential signaling pathways involved in the osteogenic/odontogenic differentiation. HBD4 was highly expressed in SHED stimulated by TNF-α and IL-1α. HBD4 could bind to LPS directly and down-regulate IL-1α, IL-1ß, IL-6, TNF-α in LPS-stimulated SHED, thus the activation of MAPK pathway decreased. HBD4 was sensitive to P. gingivalis and enhanced osteoblast/odontoblast differentiation potential of SHED by modulating Notch pathway. HBD4 was highly expressed in SHED stimulated by proinflammatory cytokines, and possessed anti-inflammatory, anti-bacterial activity. HBD4 promoted osteogenic/odontogenic differentiation of SHED. HBD4 may thus represent a suitable agent for vital pulp therapy in future clinic application.

6.
Dent Traumatol ; 2019 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-31638331

RESUMO

BACKGROUND/AIM: Data on the clinical outcomes of traumatically intruded, young, permanent teeth in Chinese children and adolescents are absent. The aim of this study was to examine the probability of spontaneous re-eruption of injured teeth, to investigate the incidence of pulp necrosis with infection of the root canal system and replacement root resorption and to analyse possible factors related to healing complications after injury. MATERIALS AND METHODS: Clinical data from 6- to 18-year-old patients who sustained intrusive luxation from 2007 to 2016 were reviewed. Teeth were treated by awaiting re-eruption, orthodontic repositioning or surgical repositioning. The incidences of spontaneous re-eruption, pulp necrosis with infection, replacement resorption and marginal bone loss were calculated, and factors related to these complications were analysed using Cox regression and Kaplan-Meier analyses. RESULTS: Data from 79 teeth in 58 patients (mean age 9.19 ± 2.34 years) were examined over follow-up periods from 7 to 87 months (median 18 months). Of the 50 teeth awaiting re-eruption, the incidences of complete re-eruption and partial re-eruption were 40.0% and 34.0%, respectively. Teeth intruded <3 mm had a higher complete re-eruption rate (57.1%) than those with a 3-7 mm of intrusion (18.2%) (hazard ratio [HR] = 4.15). Of the 52 teeth observed for more than 12 months, pulp necrosis with infection, replacement resorption and marginal bone loss occurred in 57.4%, 15.4% and 61.5% of the teeth, respectively. Teeth with 3-7 mm (60.9%, HR = 2.97) or >7 mm (100%, HR = 6.44) of intrusion and teeth with uncomplicated crown fracture (85.7%, HR = 5.19) were more likely to develop pulp necrosis with infection. Teeth that received orthodontic or surgical repositioning showed higher incidences of replacement resorption (23.1%, HR = 5.72; 25.0%, HR = 11.68, respectively). CONCLUSIONS: Spontaneous re-eruption of intruded teeth was significantly related to intrusion depth. Intrusion depth and crown fracture had strong relationships with pulp necrosis with infection, whereas the choice of treatment influenced the development of replacement resorption.

7.
Stem Cells Dev ; 28(22): 1514-1526, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31544584

RESUMO

Retinitis pigmentosa (RP) is a hereditary disease characterized by degeneration and the loss of photoreceptors. Stem cell-based therapy has emerged as a promising strategy for treating RP. Stem cells from exfoliated deciduous teeth (SHEDs), a type of mesenchymal stem cell from human exfoliated deciduous teeth, have the potential to differentiate into photoreceptor-like cells under specific induction in vitro. It has been confirmed that through paracrine secreta, SHEDs exert neurotrophic, angiogenic, immunoregulatory, and antiapoptotic functions in injured tissues. This study was designed to determine whether retinal-differentiated SHEDs and the conditioned medium derived from SHEDs (SHEDs-CM) have therapeutic effects in a mouse model of RP. The results showed that both SHEDs and SHEDs-CM improved electroretinogram responses, ameliorated photoreceptor degeneration, and maintained the structure of the outer segments of photoreceptors. The therapeutic effects were related to antiapoptotic activity of SHEDs and SHEDs-CM. Thus, SHEDs may be a promising stem cell source for treating retinal degeneration.

8.
J Oral Maxillofac Surg ; 77(12): 2503-2511, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31401026

RESUMO

PURPOSE: An intra-articular injury in growing patients should be well understood because it can interfere with normal temporomandibular joint (TMJ) function and even mandibular growth. The present study evaluated TMJ soft tissue injury after acute nondislocated and dislocated intracapsular condylar fractures (ICFs) in children. PATIENTS AND METHODS: In the present cross-sectional study, we enrolled a sample of children younger than 12 years old with acute ICFs. The predictor variable was the position of the ICF in relationship to the articular fossa, grouped as nondislocated and dislocated. The primary outcome variable was the injury type, including anterior displacement and deformity of the disc, joint effusion, and retrodiscal attachment tear. The second outcome variable was the injury score classified according to the injury type. Other study variables included age, gender, laterality, and the presence of concomitant fractures. The data were analyzed using the χ2 test and logistic regression analysis. P values < .05 were considered to indicate statistical significance. RESULTS: A total of 44 children with 63 ICFs were included, of which 28 and 35 were nondislocated and dislocated fractures, respectively. Of the 63 ICFs, 56 (88.9%) had anterior disc displacement, 2 (3.2%) had disc deformity, 59 (93.7%) had joint effusion, and 11 (17.5%) had a retrodiscal attachment tear. According to the injury type, 4 joints were given a score of as 0, 3 a score of 1, 45 a score of 2, and 11 a score of 3. The injury type and score were significantly associated with fracture dislocation (P < .05). CONCLUSIONS: In children with ICF, the presence of a retrodiscal attachment tear and disc deformity were less frequent. The type and severity of the soft tissue injury were associated with fracture dislocation. Additional studies are warranted to evaluate how soft tissue injuries affect TMJ function and fracture healing.

9.
ACS Omega ; 4(3): 5630-5639, 2019 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-31459717

RESUMO

Tetrathiafulvalene vinylogues (TTFVs) functionalized with diaryl substituents (aryl = 1-napthyl, 9-anthryl, and 1-pyrenyl) via click chemistry have been previously synthesized and studied as tweezer-type receptors for binding with C60 and C70 fullerenes. In particular, dianthryl-TTFV exhibits unique selectivity for C70 fullerene, giving rise to effective fluorescence turn-on sensing of C70 in the presence of a large excess of C60 fullerene. This observation indicated that dianthryl-TTFV has a preferential binding affinity for C70 over C60 fullerene, but the reason for such selectivity is unclear. Aiming at addressing this issue, we herein investigated the relative conformational stability of diaryl-substituted TTFVs in complexation with C70 and C60 fullerenes. The dispersion-corrected density functional theory approximation (B3LYP-D3) was employed in our computational analysis to determine binding energies and electronic properties of these supramolecular complexes. It was found that the highest binding energies (and the lowest relative conformational energies) are in pairings when fullerenes are placed around the central TTFV moieties (such as the triazole rings). The results of electronic properties show that the dianthryl-TTFV and dipyrenyl-TTFV conformers have lower highest occupied molecular orbital-lowest unoccupied molecular orbital gaps relative to the ones obtained for dinaphthyl-TTFV, indicating that dianthryl-TTFV, and to some extend dipyrenyl-TTFV, could be good candidates for chemical sensing of fullerenes with fluorescence spectroscopy. We also investigated the effect of the solvent on the interactions of the diaryl-TTFVs with fullerenes using the polarizable continuum model. In general, the presence of a solvent decreases the diaryl-TTFV/fullerene binding energies, presumably because of the interactions of the solvent with individual fullerenes and diaryl-TTFVs.

10.
Angew Chem Int Ed Engl ; 58(27): 9166-9170, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31074924

RESUMO

A new synthetic strategy (contractive annulation) for the synthesis of highly strained cyclophanes has been conceived and its viability has been demonstrated through a nine-step synthesis of [2](6,1)naphthaleno[1]paracyclophane from [2.2]paracyclophane.

11.
Diabetol Metab Syndr ; 11: 38, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31131042

RESUMO

Objective: To evaluate the therapeutic potential of stem cells from human exfoliated deciduous teeth (SHED) for diabetic peripheral neuropathy. Methods: The biological characteristics of SHED were identified by flow cytometric study and evaluation of differentiation potential. Using high-fat feeding, diabetes was induced in GK rats, and SHED were transplanted into the caudal veins of these rats. Immunohistochemical analysis was used to compare the capillary to muscle fiber ratio and intra-epidermal nerve fiber density between SHED- and saline-treated diabetic rats. Further, the expressions of angiogenesis-related and neurotrophic factors were quantified by real-time PCR and western blot. Results: SHED had a capacity of multiple differentiation and shared typical characteristics of mesenchymal stem cells. SHED transplantation relieved diabetic neuropathic pain, enabled functional recovery of the peripheral nerves, and increased the capillary to muscle fiber ratio and intra-epidermal nerve fiber density compared to the saline group and normal controls. Real-time PCR results showed that the expressions of CD31, vWF, bFGF, NGF, and NT-3 in the skeletal muscles were higher in the SHED group than in the saline groups. Western blot results indicated that the levels of the CD31 and NGF proteins were higher in the SHED transplantation group than the saline group. Conclusion: SHED transplantation ameliorated diabetic peripheral neuropathy in diabetic GK rats. Thus, systemic application of SHED could be a novel strategy for the treatment of diabetic peripheral neuropathy.

12.
BMC Genomics ; 20(1): 311, 2019 Apr 23.
Artigo em Inglês | MEDLINE | ID: mdl-31014254

RESUMO

BACKGROUND: Integration of T-DNA into plant genomes via Agrobacterium may interrupt gene structure and generate numerous mutants. The T-DNA caused mutants are valuable materials for understanding T-DNA integration model in plant research. T-DNA integration in plants is complex and still largely unknown. In this work, we reported that multiple T-DNA fragments caused chromosomal translocation and deletion in a birch (Betula platyphylla × B. pendula) T-DNA mutant yl. RESULTS: We performed PacBio genome resequencing for yl and the result revealed that two ends of a T-DNA can be integrated into plant genome independently because the two ends can be linked to different chromosomes and cause chromosomal translocation. We also found that these T-DNA were connected into tandem fragment regardless of direction before integrating into plant genome. In addition, the integration of T-DNA in yl genome also caused several chromosomal fragments deletion. We then summarized three cases for T-DNA integration model in the yl genome. (1) A T-DNA fragment is linked to the two ends of a double-stranded break (DSB); (2) Only one end of a T-DNA fragment is linked to a DSB; (3) A T-DNA fragment is linked to the ends of different DSBs. All the observations in the yl genome supported the DSB repair model. CONCLUSIONS: In this study, we showed a comprehensive genome analysis of a T-DNA mutant and provide a new insight into T-DNA integration in plants. These findings would be helpful for the analysis of T-DNA mutants with special phenotypes.


Assuntos
Betula/genética , DNA Bacteriano/genética , Rearranjo Gênico/genética , Mutação , Cromossomos de Plantas/genética , Quebras de DNA de Cadeia Dupla , Genoma de Planta/genética
13.
Stem Cells Int ; 2019: 2562981, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30906327

RESUMO

Retinal degeneration is characterized by the progressive loss of photoreceptors, and stem cell therapy has become a promising strategy. Many studies have reported that mesenchymal stem cell transplantation can sustain retinal structure and prolong retinal functions based on two mechanisms. One is cell replacement, and the other is the paracrine action of stem cells. Cells from human exfoliated deciduous teeth (SHEDs) show characteristics typical of mesenchymal stem cells. They are derived from the neural crest and are a potential cellular source for neural regeneration in stem cell therapy. In this study, we explored the potential of SHEDs to be induced towards the retinal photoreceptor phenotype and to be sustainable in an animal model of retinal degeneration. A factor-cocktail protocol was used to induce SHEDs towards retinal photoreceptors for 24 days, and the characteristics of the induced cells were identified in terms of morphological changes, biomarker expression and subcellular distribution, and calcium influx. SHEDs were labeled with firefly luciferase for in vivo tracking by bioluminescent imaging and then transplanted into the subretinal space of mice. Our results showed that SHEDs successfully transdifferentiated into photoreceptor-like cells, which displayed neuron-like morphology, and expressed specific genes and proteins associated with retinal precursors, photoreceptor precursors, and mature photoreceptors. In addition, calcium influx was significantly greater in the retinal-induced than in noninduced SHEDs. In vivo tracking confirmed at least 2 weeks of good survival by bioluminescent imaging and 3 months of sustainability of SHEDs by histological analysis. We conclude that SHEDs have the potential to transdifferentiate into retinal photoreceptor-like cells in vitro and maintain good viability in vivo after transplantation into mice with a normal immune system. This demonstrates preliminary success in generating photoreceptor-like cells from SHEDs and applying SHEDs in treating retinal degeneration.

14.
J Exp Bot ; 70(12): 3125-3138, 2019 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-30921458

RESUMO

Birch (Betula platyphylla × B. pendula) is an important tree for landscaping due to its attractive white bark and straight trunk. In this study, we characterized a T-DNA yellow-green leaf mutant, yl. We identified six insertion sites (ISs) in the mutant by genome resequencing and found a 40-kb deletion containing BpGLK1 around IS2 on chromosome 2. Complementation experiments with the yl mutant and repression of BpGLK1 in wild-type plants confirmed that BpGLK1 was responsible for the mutated phenotype. Physiological and ultrastructural analyses showed that the leaves of the yl mutant and BpGLK1-repression lines had decreased chlorophyll content and defective chloroplast development compared to the wild-type. Furthermore, the loss function of BpGLK1 also affected photosynthesis in leaves. Transcriptomics, proteomics, and ChIP-PCR analysis revealed that BpGLK1 directly interacted with the promoter of genes related to antenna proteins, chlorophyll biosynthesis, and photosystem subunit synthesis, and regulated their expression. Overall, our research not only provides new insights into the mechanism of chloroplast development and chlorophyll biosynthesis regulated by BpGLK1, but also provides new transgenic birch varieties with various levels of yellowing leaves by repressing BpGLK1 expression.

15.
Diabetol Metab Syndr ; 11: 22, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30858895

RESUMO

Background: By 2030, diabetes mellitus (DM) will be the 7th leading cause of death worldwide. Type 2 DM (T2DM) is the most common type of DM and is characterized by insulin resistance and defective ß-cell secretory function. Stem cells from human exfoliated deciduous teeth (SHED) are favorable seed cells for mesenchymal stem cells (MSCs)-based therapy due to their higher proliferation rates and easier access to retrieval. Currently, no study has revealed the therapeutic efficiency of MSCs for T2DM in Goto-Kakizaki (GK) rats. Hence, we aimed to explore the effect of SHED on T2DM in GK rats. Materials and methods: We investigated the effects of SHED on the progression of T2DM in GK rats. SHED and bone marrow mesenchymal stem cells (BMSCs) were injected via the tail vein. Body weight, fasting blood glucose and non-fasting blood glucose were measured before and after administration. At 8 weeks after injection, intraperitoneal insulin tolerance tests (IPITTs) and insulin release tests (IRTs) were performed. Additionally, hematoxylin-eosin (HE) staining, periodic acid-Schiff (PAS) staining and double-label immunofluorescence staining were used to explore the pathological changes in pancreatic islets and the liver. Immunohistochemistry (IHC) was employed to detect SHED engraftment in the liver. Additionally, real-time PCR and western blotting were used to explore glycogen synthesis, glycolysis and gluconeogenesis in the liver. Results: At 8 weeks after SHED injection, T2DM was dramatically attenuated, including hyperglycemia, IPGTT and IRT. Additionally, histological analysis showed that SHED injection improved pancreatic islet and liver damage. Real-time PCR analysis showed that SHED significantly reversed the diabetic-induced increase of G-6-Pase, Pck1 and PK; and significantly reversed the diabetic-induced decrease of GSK3ß, GLUT2, and PFKL. In addition, western blotting demonstrated that SHED significantly reversed the diabetic-induced increase of G-6-Pase and reversed the diabetic-induced decrease of GLUT2, GSK3ß and PFKM. Conclusion: Stem cells from human exfoliated deciduous teeth offers a potentially effective therapeutic modality for ameliorating T2DM, including hyperglycemia, insulin resistance, pancreatic islets and liver damage, and decreased glycogen synthesis, inhibited glycolysis and increased gluconeogenesis in the liver.

16.
ACS Appl Mater Interfaces ; 11(14): 13257-13263, 2019 Apr 10.
Artigo em Inglês | MEDLINE | ID: mdl-30810309

RESUMO

Silicon (Si) remains one of the most promising anode materials for next-generation lithium-ion batteries (LIBs). The key challenge for Si anodes is the huge volume change during lithiation-delithiation cycles that leads to electrode pulverization and rapid capacity fading. Here, we report a hierarchical porous Si (hp-Si) with a tailored porous structure [tunable primary pores (20-200 nm) and secondary nanopores (∼3-10 nm)] that can effectively minimize the volume expansion. An in situ transmission electron microscopy (TEM) study revealed that the hp-Si material with the same porosity but larger primary pores can more effectively accommodate lithiation-induced volume expansion, giving rise to a much reduced apparent volume expansion on both material and electrode levels. Chemomechanical modeling revealed that because of the different relative stiffnesses of the lithiated and unlithiated Si phases, the primary pore size plays a key role in accommodating the volume expansion of lithiated Si. The higher structural stability of the hp-Si materials with larger primary pores also maintains the fast diffusion channels of the connective pores, giving rise to better power capability and capacity retention upon electrochemical cycling. Our findings point toward an optimized hp-Si material with minimal volume change during electrochemical cycling for next-generation LIBs.

17.
Oncol Lett ; 17(2): 1889-1895, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30675252

RESUMO

Clinical management of metastatic prostate cancer remains a challenge. Activation of apoptosis signaling pathways via signal transducer and activator of transcription 6 (STAT6) has been hypothesized to be a therapeutic strategy for patients with metastatic prostate cancer. The ONCOMINE® prostate cancer database and two Gene Expression Omnibus datasets (Gene Series 40026 and 21032) were re-analyzed to determine the expression levels of STAT6 and microRNA (miR)-135a in prostate cancer. The current study investigated the induced overexpression of miR-135a in prostate cancer cell lines to detect its function in prostate cell apoptosis using Hoechst staining and fluorescence-activated cell sorting and examined the expression levels of STAT6 and its DNA binding ability using western blotting and an electrophoretic mobility shift assay. In analysis of the ONCOMINE® database, STAT6 expression levels in prostate cancer tissue were higher compared with those in normal prostate gland tissue and were associated with the overall survival rate and biochemical relapse rate following radical prostatectomy. Additionally, there was an inverse correlation between miR-135a and STAT6 expression levels in prostate cancer cell lines. miR-135a was able to induce prostate cancer cell apoptosis via targeting STAT6 mRNA and subsequently repressing protein expression and phosphorylation, which also altered the transcriptional factor function of STAT6 through its DNA-binding capabilities. In conclusion, miR-135a may function as a tumor-suppressing miRNA in prostate cancer and its anti-oncogenic activity may involve the direct targeting and inhibition of STAT6.

18.
Neurochem Int ; 124: 94-105, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30629983

RESUMO

In China, L-3-n-butylphthalide (L-NBP) showed promising pharmacological actions in stroke treatment. Analyzing the characteristics of L-NBP might provide valuable hints for new drug design. The current study is aimed to determine the effects of L-NBP on neuritogenesis and further to elucidate the neuronal protection against stroke impairment in vitro. L-NBP was applied to rat pheochromocytoma PC12 cells and cultured rat cortical neurons under the normoxic condition and the oxygen-glucose deprivation/reoxygenation (OGD/R) insults, respectively. Immunofluorescence staining, western blot analysis, Sholl analysis, lactate dehydrogenase (LDH) release assay, 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) reduction assay and enzyme-linked immunosorbent assay (ELISA) were performed. L-NBP could concentration-dependently stimulate the development of growth cones, enhance the neuritic branches and synapse formation. It indicated that L-NBP possibly promoted the neuritogenic activity in a stage-dependent manner. Further research proved that L-NBP could promptly activate epidermal growth factor (EGF) receptor, up-regulate the expressions of extracellular signal-regulated kinase1/2 (ERK1/2), cAMP response element-binding protein (CREB) and E-26-like protein 1 (ELK-1). In addition, L-NBP enhanced the sustained expressions of brain derived neurotrophic factor (BDNF) and nerve growth factor (NGF). The inhibition to the receptors of EGF, NGF,BDNF could attenuate L-NBP induced neuritogenic and neuronal survival after the OGD/R toxicity. Basing on these investigations, we concluded that L-NBP might reconstruct the impaired neuronal network and improved the neuronal complexity after the ischemic insults through multiple pathways which at least were via the activations of EGF receptor, BDNF and NGF related signals.


Assuntos
Benzofuranos/farmacologia , Medicamentos de Ervas Chinesas/farmacologia , Neuritos/efeitos dos fármacos , Neurogênese/efeitos dos fármacos , Neurônios/efeitos dos fármacos , Fármacos Neuroprotetores/farmacologia , Animais , Hipóxia Celular/efeitos dos fármacos , Hipóxia Celular/fisiologia , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/fisiologia , Células Cultivadas , Relação Dose-Resposta a Droga , Neuritos/fisiologia , Neurogênese/fisiologia , Neurônios/fisiologia , Células PC12 , Ratos , Ratos Sprague-Dawley
19.
Chempluschem ; 84(6): 754-765, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31944011

RESUMO

A monomer-through-pentamer series of oligo(1,8-pyrenylene)s was synthesized using a two-step iterative synthetic strategy. The trimer, tetramer, and pentamer are mixtures of atropisomers that interconvert slowly at room temperature (as shown by variable-temperature NMR analysis). They are liquids well below room temperature, as indicated by POM, DSC and SWAXS analysis. These oligomers are highly fluorescent both in the liquid state and in dilute solution (λF,max = 444-457 nm, φF = 0.80) and an investigation of their photophysical properties demonstrated that delocalization plays a larger role in their excited states than it does in related pyrene-based oligomers.

20.
Sci Rep ; 8(1): 17031, 2018 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-30429517

RESUMO

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.

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