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1.
Microb Drug Resist ; 2020 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-32013730

RESUMO

Background: Septicemia in children in mainland China has recently become a public health concern. Methods: A meta-analysis was performed on studies investigating the prevalence of cephalosporin-resistant Escherichia coli isolated from children with septicemia in mainland China from 2007 to 2017 following a search of relevant databases. Results: A total of 43 articles reporting 11 cephalosporins were included in the review. The results of the meta-analysis revealed that for the first-generation cephalosporins, the pooled summarized prevalence of resistance to cefazolin was 74.96% (95% confidence interval [CI]: 64.79-83.91) and cephalothin resistance was 62.28% (95% CI: 36.45-100). Regarding the second-generation cephalosporins, cefoxitin-resistant E. coli comprised 23.85% (95% CI: 10.60-40.40) and cefuroxime resistance was 60.32% (95% CI: 51.25-68.73). For the third-generation cephalosporins, the pooled summarized prevalence of resistance was 51.34% for cefotaxime (95% CI: 40.08-62.54), 40.43% for ceftazidime (95% CI: 31.07-50.15), 45.51% for cefoperazone (95% CI: 20.41-70.61), 12.10% for cefoperazone/sulbactam (95% CI: 6.55-18.76), 62.99% for ceftriaxone (95% CI: 55.00-70.98), and 0% for cefotetan. Among the fourth-generation cephalosporins, resistance to cefepime was 34.08% (95% CI: 25.91-43.31). Conclusions: Most third-generation cephalosporins (e.g., cefotaxime and ceftriaxone) retained high resistance rates throughout the 11-year study period without significant changes. The new fourth-generation cephalosporin, cefepime, is rapidly gaining resistance. Interestingly, ceftazidime, cefepime, and cefoperazone/sulbactam showed a recent decreasing trend of drug resistance. These situations may present a risk for treating children with septicemia and should be closely monitored and treated.

2.
PLoS One ; 15(2): e0228388, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32045422

RESUMO

BACKGROUND: Amphenicols have been widely used in the pig industry in China, leading to varying degrees of drug resistance. METHODS: The systematic review was performed according to PRISMA (Preferred Reported Items for Systematic Reviews and Meta-Analysis) recommendations on studies investigating the prevalence of amphenicol-resistant Escherichia coli (E. coli) isolated from pig in mainland China from 2000 to 2018, a random-effects model was selected, then followed by meta-analysis. RESULTS: A total of 103 articles were included in the study. The results of the meta-analysis revealed that the pooled summarized prevalence of resistance to chloramphenicol (CAP) was 72.31% (95% confidence interval (95% CI) = 67.12%-77.23%) and to florfenicol (FF) was 58.64% (95% CI = 52.48%-64.67%). During the past 18 years, the resistance rate to CAP remained high initially but then declined rapidly after 2012, whereas the resistance rate to FF plateaued (54.13%-59.60%) from 2000-2018. In different parts of China, the rate of resistance to amphenicols among E. coli isolates was fairly consistent, with the exception of the north and northwest regions. CONCLUSIONS: In 2002, the veterinary use of CAP was prohibited and its resistance levels in E. coli isolated from pigs was initially maintained at a high level but then showed an obvious downward trend in recent years. Resistance to commonly used FF remained at a high but stable level.

3.
Res Vet Sci ; 127: 82-90, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31678457

RESUMO

Pasteurella multocida possesses a polysaccharide capsule composed of a viscous surface layer that acts as a critical structural component and virulence factor. Capsular polysaccharides are structurally similar to vertebrate glycosaminoglycans, providing an immunological mechanism for bacterial molecular mimicry, resistance to phagocytosis, and immune evasion during the infection process. In recent years, a series of important research advances have been made in understanding the biosynthesis and regulatory aspects of the P. multocida capsule. This review systematically examines the serogroups, polysaccharide composition and structures, biosynthetic loci and functions, biosynthesis pathways, and expression regulation mechanisms of the P. multocida capsule, supplying a theoretical basis for the molecular pathogenesis of the P. multocida capsule and the future development of capsular polysaccharide vaccines.


Assuntos
Cápsulas Bacterianas/metabolismo , Vacinas Bacterianas/química , Pasteurella multocida/metabolismo , Polissacarídeos/biossíntese , Fatores de Virulência/metabolismo , Pasteurella multocida/genética , Pasteurella multocida/patogenicidade , Sorogrupo
4.
Microb Pathog ; 135: 103620, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31310833

RESUMO

NDM-1-producing Enterobacteriaceae are multidrug-resistant bacteria, also called superbacteria, that have become important global human health threats in recent years. However, data about NDM-1-producing bacteria in animals are rare. In this study, an NDM-1-producing Escherichia coli isolate (designated E120413) was obtained from pigs in Henan province, China in 2012. The susceptibility of E. coli E120413 to antimicrobial agents was determined using Kirby-Bauer disk diffusion and micro-dilution methods. Susceptibility tests indicated that E. coli E120413 was resistant to almost all common antibiotics with high MIC values obtained for most antibiotics tested. E. coli E120413 was detected in the heart, liver, spleen, lung, kidney, brain, stomach, duodenum, mesenteric lymph nodes, and fecal samples of piglets in both cohabitation and experimental groups and the bacteria persisted for more than 2 weeks. However, no obvious clinical symptoms or serious pathological lesions were observed. This is the first investigation of NDM-1-producing E. coli isolate from pigs in China. Although no significant pathological lesions were observed, NDM-1-producing E. coli was found to be highly transmissible and to cause persistent infection in pigs.


Assuntos
Infecções por Enterobacteriaceae/microbiologia , Escherichia coli/enzimologia , Escherichia coli/isolamento & purificação , Suínos/microbiologia , beta-Lactamases/biossíntese , Animais , Antibacterianos/farmacologia , China , Modelos Animais de Doenças , Testes de Sensibilidade a Antimicrobianos por Disco-Difusão , Farmacorresistência Bacteriana Múltipla/efeitos dos fármacos , Infecções por Enterobacteriaceae/patologia , Escherichia coli/efeitos dos fármacos , Escherichia coli/genética , Fezes/microbiologia , Testes de Sensibilidade Microbiana , Virulência , beta-Lactamases/genética
5.
Biotechnol Lett ; 41(6-7): 651-673, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31020454

RESUMO

The 2,5-diketopiperazines (DKPs) are the smallest cyclopeptides and their basic structure includes a six-membered piperazine nucleus. Typical peptides lack a special functional group in the oligopeptide nucleus. Both are produced by at least 35 representative genera of fungi, and possess huge potential as pharmaceutical drugs and biocontrol agents. To date, only cyclosporin A has been developed into a commercial product. This review summarises 186 fungi-derived compounds reported since 2000. Antibiotic (antibacterial, antifungal, synergistic antifungal, antiviral, antimycobacterial, antimalarial, antileishmanial, insecticidal, antitrypanosomal, nematicidal and antimicroalgal) activities are discussed for 107 of them, including 66 DKPs (14 epipolythiodioxopiperazines, 20 polysulphide bridge-free thiodiketopiperazines, and 32 sulphur-free prenylated indole DKPs), 15 highly N-methylated, and 26 non-highly N-methylated typical peptides. Structure-activity relationships, mechanisms of action, and research methods are covered in detail. Additionally, biosynthases of tardioxopiperazines and neoechinulins are highlighted. These compounds have attracted considerable interest within the pharmaceutical and agrochemical industries.


Assuntos
Anti-Infecciosos/farmacologia , Descoberta de Drogas/tendências , Fungos/metabolismo , Peptídeos Cíclicos/farmacologia , Anti-Infecciosos/isolamento & purificação , Anti-Infecciosos/metabolismo , Peptídeos Cíclicos/isolamento & purificação , Peptídeos Cíclicos/metabolismo
6.
Peptides ; 113: 52-65, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30738838

RESUMO

Lipopeptide antibiotics have linear or cyclic structures with one or more hydrocarbon tails linked to the N-terminus of a short oligopeptide that may be chemically modified and/or contain unusual amino acid residues in their structures. They possess huge potential as pharmaceutical drugs and biocontrol agents, and ˜30 representative genera of fungi are known to produce them. Some chemically synthesised derivatives have already been developed into commercial products or subjected to clinical trials, including cilofungin, caspofungin, micafungin, anidulafungin, rezafungin, emodepside, fusafungine and destruxins. This review summarizes 200 fungi-derived compounds reported since 2000, including 95 cyclic depsipeptides, 67 peptaibiotics (including 35 peptaibols, eight lipoaminopeptides, and five lipopeptaibols), and 38 non-depsipeptide and non-peptaibiotic lipopeptides. Their sources, structural sequences, antibiotic activities (e.g. antibacterial, antifungal, antiviral, antimycobacterial, antimycoplasmal, antimalarial, antileishmanial, insecticidal, antitrypanosomal and nematicidal), structure-activity relationships, mechanisms of action, and specific relevance are discussed. These compounds have attracted considerable interest within the pharmaceutical and agrochemical industries.


Assuntos
Anti-Infecciosos/farmacologia , Lipopeptídeos/farmacologia , Anidulafungina/farmacologia , Caspofungina/farmacologia , Depsipeptídeos/farmacologia , Equinocandinas/farmacologia , Proteínas Fúngicas/farmacologia , Fungos/química , Micafungina/farmacologia , Relação Estrutura-Atividade
7.
Peptides ; 107: 17-24, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-30077717

RESUMO

Members of cyanobacteria, including Moorea spp., Okeania spp., Lyngbya spp., Schizothrix spp., Leptolyngbya spp., Microcystis spp., Symploca spp., Hassallia sp., Anabaena spp., Planktothrix sp., Tychonema spp., Oscillatoria spp., Tolypothrix sp., Nostoc sp., and Hapalosiphon sp. produce an enormously diverse range of peptide antibiotics with huge potential as pharmaceutical drugs and biocontrol agents following screening of structural analogues and analysis of structure-activity relationships (SAR). The need for novel antibiotic lead compounds is urgent, and this review summarizes 78 cyanobacteria-derived compounds reported since 2000, including 32 depsipeptides, 18 cyclic lipopeptides, 13 linear lipopeptides, 14 cyclamides, and one typical cyclic peptide. The current and potential therapeutic applications of these peptides are discussed, including for SAR, antituberculotic, antifungal, antibacterial, antiviral, and antiparasitic (anti-plasmodial, antitrypanosomal and antileishmanial) activities.


Assuntos
Anti-Infecciosos , Cianobactérias/química , Antibacterianos , Cianobactérias/metabolismo , Depsipeptídeos , Lipopeptídeos , Peptídeos , Peptídeos Cíclicos , Relação Estrutura-Atividade , Compostos de Sulfonilureia
8.
Sci Total Environ ; 625: 486-495, 2018 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-29291563

RESUMO

Life cycle assessment methodology was used to quantify the environmental impacts and resource use of milk production on the North China Plain, the largest milk production area in China. Variation in environmental burden caused by cow productivity was evaluated, as well as scenario analysis of the effects of improvement practices. The results indicated that the average environmental impact potential and resource use for producing 1kg of fat and protein corrected milk was 1.34kgCO2eq., 9.27gPO43-eq., 19.5gSO2eq., 4.91MJ, 1.83m2 and 266L for global warming potential (GWP), eutrophication potential (EP), acidification potential (AP), non-renewable energy use (NREU), land use (LU) and blue water use (BWU; i.e. water withdrawal), respectively. Feed production was a significant determinant of GWP, NREU, LU and BWU, while AP and EP were mainly affected by manure management. Scenario analysis showed that reducing use of concentrates and substituting with alfalfa hay decreased GWP, EP, AP, NREU and LU (by 1.0%-5.5%), but caused a significant increase of BWU (by 17.2%). Using imported soybean instead of locally-grown soybean decreased LU by 2.6%, but significantly increased GWP and NREU by 20% and 6.9%, respectively. There was no single perfect manure management system, with variable effects from different management practices. The environmental burden shifting observed in this study illustrates the importance of assessing a wide range of impact categories instead of single or limited indicators for formulating environmental policies, and the necessity of combining multiple measures to decrease the environmental burden. For the North China Plain, improving milking cow productivity and herd structure (i.e. increased proportion of milking cows), combining various manure management systems, and encouraging dairy farmers to return manure to nearby crop lands are promising measures to decrease multiple environmental impacts.


Assuntos
Indústria de Laticínios , Meio Ambiente , Aquecimento Global , Animais , Bovinos , China , Feminino , Esterco , Leite
9.
J Vet Diagn Invest ; 30(2): 245-248, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29105585

RESUMO

Citrobacter freundii is considered a ubiquitous organism and an opportunistic pathogen. Reports of C. freundii-associated disease in mammals have been very limited. We report an outbreak of C. freundii septicemia and encephalitis in sheep, with a high mortality rate; 13 adult sheep were found dead over a 6-d period on a farm in central China that housed ~1,370 sheep. Three animals were autopsied and showed septicemia, congestion of meningeal vessels, and pleural effusion. C. freundii was isolated in abundance in pure culture from 19 of 21 organs. All 3 C. freundii isolates had similar antimicrobial resistance phenotypes for 10 of the 11 agents tested, and were sensitive to 8 of the 11 agents. We reproduced C. freundii infection in sheep experimentally by oral or subcutaneous inoculation routes, and recovered the challenge organism from all of the experimentally infected sheep. Intramuscular injection of enrofloxacin protected sheep against an otherwise fatal challenge. Our results suggest that C. freundii played a major role in this disease outbreak.


Assuntos
Citrobacter freundii/isolamento & purificação , Surtos de Doenças/veterinária , Encefalite/veterinária , Infecções por Enterobacteriaceae/veterinária , Sepse/veterinária , Doenças dos Ovinos/mortalidade , Animais , China/epidemiologia , Encefalite/epidemiologia , Encefalite/mortalidade , Infecções por Enterobacteriaceae/epidemiologia , Infecções por Enterobacteriaceae/mortalidade , Feminino , Testes de Sensibilidade Microbiana/veterinária , Sepse/epidemiologia , Sepse/mortalidade , Ovinos , Doenças dos Ovinos/epidemiologia , Doenças dos Ovinos/microbiologia
10.
Vet Immunol Immunopathol ; 186: 51-54, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28413050

RESUMO

This study evaluated whether there was an association between polymorphisms within the Toll-like receptor 2 gene (TLR2) of Chinese Holstein cattle and susceptibility to bovine tuberculosis (BTB). In a case-control study including 210 BTB cases and 237 control cattle, we found only two common single-nucleotide polymorphisms (SNPs) within the entire coding region of the TLR2 gene, A631G (rs95214857) and T1707C (rs1388116488). Additionally, the allele and genotype distributions of A631G and T1707C were not different between case and control groups, indicated that these SNPs were not associated with susceptibility to BTB. These results suggested that polymorphisms in the TLR2 gene might not play a significant role in the BTB risk in Chinese Holstein cattle.


Assuntos
Polimorfismo de Nucleotídeo Único , Receptor 2 Toll-Like/genética , Tuberculose Bovina/genética , Animais , Bovinos , China
11.
Genet Test Mol Biomarkers ; 21(5): 322-327, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-28346829

RESUMO

AIMS: Individual sensitivity to glucocorticoid (GC) therapy might play a pivotal role in the development of GC-induced avascular necrosis of the femoral head (GANFH). In a growing number of studies, common polymorphisms of the glucocorticoid receptor gene (nuclear receptor subfamily 3 group C member 1 [NR3C1]) have been associated with variability in the individual sensitivity to GCs. However, whether the NR3C1 gene polymorphisms actually influence the susceptibility of GANFH remains unknown. METHODS: In this study, we report the findings of a case-control study to investigate the role of the NR3C1 gene single-nucleotide polymorphisms (SNPs) in GANFH susceptibility among 78 GANFH patients (GCs sensitive) and 115 GC-resistant controls. RESULTS: Our results found no significant associations between the SNPs N363S, Tth111I, BclI, ER22/23EK, and A3669G with GANFH susceptibility. The G allele frequency, both homozygous and heterozygous, of SNP BclI was significantly different between control and GANFH combined with osteopenia subgroups (odds ratios [OR] = 1.81; 95% confidence intervals [CI] = 1.05-3.10; OR = 2.04; 95% CI = 1.03-4.07, respectively). CONCLUSIONS: Most of these common SNPs in the NR3C1 gene likely do not play critical roles in the susceptibility of GANFH. However, the G allele at the SNP Bcll, irrespective of dosage, may increase risk for the development of GANFH combined with osteopenia in the Chinese population.


Assuntos
Necrose da Cabeça do Fêmur/genética , Receptores de Glucocorticoides/genética , Adulto , Alelos , Grupo com Ancestrais do Continente Asiático/genética , Estudos de Casos e Controles , China , Feminino , Cabeça do Fêmur , Frequência do Gene/genética , Estudos de Associação Genética/métodos , Predisposição Genética para Doença/genética , Glucocorticoides/uso terapêutico , Humanos , Masculino , Pessoa de Meia-Idade , Razão de Chances , Polimorfismo de Nucleotídeo Único/genética , Fatores de Risco
12.
Can J Vet Res ; 81(1): 22-27, 2017 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-28154458

RESUMO

The objective of this study was to assess the efficacy of a trivalent inactivated Haemophilus parasuis serovars 4, 5, and 12 vaccine with polymeric adjuvant gel (GEL) and commercial vaccines against Glässer's disease in piglets. Commercial vaccines containing inactivated H. parasuis serovars 4 and 5 (China), inactivated H. parasuis serovars 1 and 6 (Spain), and inactivated H. parasuis serovar 5 (USA) were also evaluated. Our results demonstrated that the trivalent inactivated H. parasuis serovars 4, 5, and 12 vaccine with GEL adjuvant can provide better protection against the 3 most common pathogenic serovars circulating in China than other commercial vaccines tested. Our findings also indicated that inactivated H. parasuis serovars 1 and 6 vaccine cross-protects piglets against H. parasuis serovars 4 and 5; inactivated H. parasuis serovar 5 vaccine cross-protects piglets against H. parasuis serovar 4 challenge; but none of the commercial vaccines tested in this study protected piglets against H. parasuis serovar 12. Our results provide a basis for further identification of common protective antigens that can induce cross-protection against heterogeneous serovars.


Assuntos
Vacinas Bacterianas/imunologia , Infecções por Haemophilus/veterinária , Haemophilus parasuis/classificação , Doenças dos Suínos/prevenção & controle , Animais , Anticorpos Antibacterianos/sangue , Vacinas Bacterianas/efeitos adversos , Feminino , Infecções por Haemophilus/microbiologia , Infecções por Haemophilus/prevenção & controle , Sorogrupo , Suínos , Vacinas de Produtos Inativados/efeitos adversos , Vacinas de Produtos Inativados/imunologia
13.
Ir Vet J ; 70: 2, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28078081

RESUMO

BACKGROUND: Prior to the 1990s, P. multocida capsular serogroup A was the most prevalent in China, followed by serogroups B and D. Thirty years later, serogroup D became the most prevalent, followed by serogroups A and B. However, the P. multocida capsular serogroups currently circulating in China remain unclear. Therefore, the aim of the present study was to provide an update on P. multocida serogroups isolated from diagnostic samples collected from clinically diseased pigs in Central and Eastern China from 2011 to 2015. RESULTS: Between February 2011 and October 2015, 296 isolates of Pasteurella multocida were collected from 3212 pigs with clinical respiratory disease in 12 provinces of China (isolation rate of 9.2%). Of the 296 collected isolates, 146 (49.3%) were P. multocida capsular type A, 141 (47.6%) were capsular type D, and one was capsular type B. Streptococcus suis (94/193; 48.7%), Haemophilus parasuis (76/193; 39.3%), Escherichia coli (53/193; 27.5%), and Bordetella bronchiseptica (26/193; 13.5%) were frequently isolated together with P. multocida. A total of 14 toxigenic P. multocida strains co-isolated with other pathogens from 32 cases of atrophic rhinitis were classified into serogroup D. The virulence of P. multocida capsular type A isolates was higher than that of capsular type D isolates based on LD50 studies in mice. CONCLUSIONS: Over the past 5 years, P. multocida capsular type A was the most frequently isolated from diagnostic submissions in Central and Eastern China, followed by serogroups D and B.

14.
Can J Vet Res ; 80(4): 287-293, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27733783

RESUMO

The objective of this study was to assess the minimum dose, antigen content, and immunization duration of a trivalent vaccine containing inactivated Haemophilus parasuis serovars 4, 5, and 12 and the Montanide GEL 01 PR adjuvant in piglets and pregnant sows. Our results demonstrated that the minimum vaccine dose was 2 mL per pig and the optimal antigen content 2.0 × 109, 1.0 × 109, and 1.0 × 109 colony-forming units/mL of serovars 4, 5, and 12, respectively. The vaccine provided effective protection 14 d after the 2nd vaccination, and the period of immune protection was 180 d (6 mo) after the 2nd vaccination. Maternal antibodies provided early protection for the piglets, and vaccinating the sows before farrowing helped to control disease and protected the piglets during lactation; the piglets were protected during the finishing period by being vaccinated during lactation. Our findings provide a basis for developing a commercial trivalent vaccine of inactivated H. parasuis serovars 4, 5, and 12 against Glässer's disease.


Assuntos
Antígenos de Bactérias/química , Vacinas Bacterianas/imunologia , Infecções por Haemophilus/veterinária , Haemophilus parasuis , Animais , Antígenos de Bactérias/imunologia , Relação Dose-Resposta Imunológica , Feminino , Infecções por Haemophilus/microbiologia , Infecções por Haemophilus/prevenção & controle , Imunização , Gravidez , Suínos , Vacinas de Produtos Inativados/imunologia
15.
Vet Immunol Immunopathol ; 180: 53-58, 2016 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-27692096

RESUMO

Glässer's disease, which is caused by Haemophilus parasuis, is a major threat to swine throughout the world. At present, the predominant method of controlling this disease is through vaccination with an inactivated vaccine, which has many limitations. For example, there is no available method to differentiate between infected and vaccinated animals. In addition, inactivated vaccines do not contain all of the virulent serovars that circulate in a region. Additionally, novel vaccines that address the shortcomings of inactivated vaccines are not available. Here, we summarize existing knowledge concerning H. parasuis vaccines, and provide some suggestions that may help to improve H. parasuis vaccines.


Assuntos
Vacinas Anti-Haemophilus/imunologia , Haemophilus parasuis/imunologia , Animais , Engenharia Genética , Suínos , Vacinas de DNA/imunologia , Vacinas de Produtos Inativados/imunologia , Vacinas de Subunidades/imunologia
16.
BMC Vet Res ; 12(1): 138, 2016 Jul 04.
Artigo em Inglês | MEDLINE | ID: mdl-27377264

RESUMO

BACKGROUND: There is no information concerning the genotype of Canine parvovirus (CPV) currently circulating in Henan province, China. Therefore, the aim of the present study was to provide insights into the epidemiology and molecular characterization of CPV circulating in Henan province from 2009 to 2014. RESULTS: Nineteen thousand nine hundred seven dogs from pet hospitals in the cities of Luoyang, Anyang, Jiaozuo, Sanmenxia, Xinxiang, Zhengzhou in Henan province between 2009 and 2014 were investigated. Over the 6-year period, 1169 CPV-positive cases were identified and the morbidity of CPV infection ranged from 4.16 to 8.06 %, although morbidity was not significant (P > 0.05) between 2009 and 2014. Factors associated with morbidity included sampling season, dog age, breed, vaccination status, and sex. CPV co-infection with coccidium (10.00 %), canine distemper virus (4.79 %), hookworm (2.40 %), canine coronavirus (1.11 %), roundworm (1.03 %), tapeworm (0.17 %) and Babesia spp. (0.09 %) were observed. The new CPV-2a variant was more prevalent than the new CPV-2b variant in Henan province. CPV 2c was not observed in this study. CONCLUSIONS: The epidemiology of CPV infection and identification of the circulating genotypes in Henan province, China from 2009 to 2014 determined that the new CPV-2a variant was more prevalent.


Assuntos
Doenças do Cão/epidemiologia , Infecções por Parvoviridae/veterinária , Animais , Proteínas do Capsídeo/genética , China/epidemiologia , Coinfecção , Cães , Feminino , Genótipo , Masculino , Tipagem Molecular , Infecções por Parvoviridae/epidemiologia , Parvovirus Canino/genética , Prevalência , Fatores de Risco
17.
Genet Test Mol Biomarkers ; 20(5): 255-60, 2016 May.
Artigo em Inglês | MEDLINE | ID: mdl-27003337

RESUMO

AIMS: A growing number of genome-wide association studies (GWAS) have revealed associations between single-nucleotide polymorphisms (SNPs) and susceptibility to tuberculosis (TB). However, the results of these studies have been inconclusive. This study evaluated whether the SNPs rs4331426 and rs2057178, identified by GWAS, are associated with TB susceptibility. METHODS: We performed meta-analyses for rs4331426, based on eight case-control studies which included a total of 4988 TB cases and 9041 controls; and rs2057178, based on five case-control studies, including a total of 9400 TB cases and 14,459 controls. RESULTS: Our meta-analyses indicated that both rs4331426 and rs2057178 were associated with increased risk of TB (G vs. A: odds ratio [OR] = 1.15, 95% confidence interval [CI]: 1.08-1.22 and A vs. G: OR = 0.84, 95% CI: 0.80-0.88, respectively), especially in an African subgroup. However, no significant TB association was found with rs4331426 in an Asian subgroup. CONCLUSIONS: These meta-analyses indicate that rs4331426 and rs2057178 might play a role in the risk of developing TB, especially in Africans; however, rs4331426 might not play a significant role in the risk of developing TB in Asians.


Assuntos
Polimorfismo de Nucleotídeo Único , Tuberculose/genética , Grupo com Ancestrais do Continente Africano/genética , Antígenos de Diferenciação de Linfócitos T/genética , Grupo com Ancestrais do Continente Asiático/genética , Proteínas de Ligação ao Cálcio/genética , Proteínas de Transporte/genética , Estudos de Casos e Controles , Genes do Tumor de Wilms , Predisposição Genética para Doença , Estudo de Associação Genômica Ampla , Humanos , Glicoproteínas de Membrana/genética , Proteínas Nucleares/genética , RNA não Traduzido/genética
18.
Wei Sheng Wu Xue Bao ; 55(7): 942-8, 2015 Jul 04.
Artigo em Chinês | MEDLINE | ID: mdl-26710613

RESUMO

OBJECTIVE: To develop a host-vector balanced lethal system of Salmonella typhimurium adenylate cyclase mutant, and detect its biological characteristics. METHODS: We constructed SL1344ΔcyaΔasd mutant strain by recombinant suicide plasmid (pREAasd), and screened by two-step method, transformed pYA3493 plasmid containing the asd gene without resistance electric into the lack of SL1344 AcyaΔasd, then the recombinant strains SL1344 ΔcyaΔasd (pYA3493) was constructed successfully. RESULTS: The biochemical characteristics and growth rate of the mutant were different from that of the wild strain SL1344, but almost the same as that of the parent strain SL1344Δcya. The mutant strain could neither ferment maltose, lactose, and sorbitol, nor decompose H2S, galactose and rat lee sugar, but still retained the ability to use glucose. The one-day chicken lethal test showed that SL1344ΔcyaΔasd (pYA3493) mutant was at least 104 times lower than SL1344 strain. The protection rate induced by the SL1344ΔcyaΔasd (pYA3493) mutant was 62. 5%. CONCLUSION: The SL1344ΔcyaΔasd (pYA3493) mutant was successfully constructed, and had good immune protection, it laid a foundation for developing potential oral vaccines.


Assuntos
Proteínas de Bactérias/genética , Vetores Genéticos/imunologia , Doenças das Aves Domésticas/imunologia , Salmonelose Animal/imunologia , Salmonella typhimurium/imunologia , Animais , Proteínas de Bactérias/administração & dosagem , Proteínas de Bactérias/imunologia , Vacinas Bacterianas/administração & dosagem , Vacinas Bacterianas/genética , Vacinas Bacterianas/imunologia , Galinhas , Feminino , Vetores Genéticos/genética , Masculino , Doenças das Aves Domésticas/microbiologia , Doenças das Aves Domésticas/prevenção & controle , Salmonelose Animal/microbiologia , Salmonelose Animal/prevenção & controle , Salmonella typhimurium/genética , Salmonella typhimurium/patogenicidade , Vacinas Atenuadas/administração & dosagem , Vacinas Atenuadas/genética , Vacinas Atenuadas/imunologia , Virulência
19.
Vet Immunol Immunopathol ; 168(3-4): 153-8, 2015 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-26672914

RESUMO

Haemophilus parasuis has had a huge impact in the swine industry throughout the world. Inactivated bacterium for H. parasuis is a traditional vaccine that can elicit efficient protection against homologous challenges. The objective of this study was to screen for the adjuvant-enhanced immune effect of trivalent inactivated H. parasuis serovars 4, 5 and 12 (prevalent serovars in China) vaccines against Glässer's disease. The adjuvants of mineral oil, aluminum hydroxide, Montanide GEL 01 PR, Montanide IMS 1313N VG and Montanide ISA 760 VG were used to make emulsified inactivated H. parasuis serovars 4, 5 and 12, respectively. Safety, antibody titer and protective efficacy of these vaccines were examined separately in piglets, and the feasibility of microagglutination test for detecting antibody titer of H. parasuis was confirmed for the first time. Due to easy of injection, high safety, rapidly immune responses, high concentrations of antibody, and 100% of protective efficacy for piglets, Montanide GEL 01 PR adjuvant can provide more homologous serovar protection than other domestically developed inactivated vaccines and should be used as a candidate adjuvant.


Assuntos
Vacinas Bacterianas/imunologia , Infecções por Haemophilus/veterinária , Haemophilus parasuis/classificação , Doenças dos Suínos/prevenção & controle , Animais , Anticorpos Antibacterianos , Vacinas Bacterianas/efeitos adversos , Tecido de Granulação/patologia , Infecções por Haemophilus/microbiologia , Infecções por Haemophilus/prevenção & controle , Haemophilus parasuis/imunologia , Suínos , Doenças dos Suínos/microbiologia
20.
Genet Test Mol Biomarkers ; 18(3): 196-201, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24387690

RESUMO

P-glycoprotein (P-gp) is present in various tissue cells, required for the pumping of lipophilic drugs (including glucocorticoids) out of cells. We hypothesized that polymorphisms in the P-gp encoding gene (multidrug-resistant transporter-1 [MDR1]) are related to individual differences in glucocorticoid sensitivity and the development of glucocorticoid-induced avascular necrosis of the femoral head (GANFH). In this case-control study, we genotyped three known single-nucleotide polymorphisms (SNPs: C1236T, G2677T/A, and C3435T) within the MDR1 gene in 662 Chinese subjects. Statistically significant differences between GANFH patients and either healthy controls or glucocorticoid-resistant patients (non-GANFH) were found for the T allele or TT genotype of C3435T. The haplotype TTT, composed of these three SNPs, exhibited a significant association with the disease. No associations were identified between C1236T or G2677T/A and GANFH. Our results suggest that the C3435T polymorphism of the MDR1 gene is associated with susceptibility to GANFH in a Chinese population.


Assuntos
Necrose da Cabeça do Fêmur/induzido quimicamente , Necrose da Cabeça do Fêmur/genética , Glucocorticoides/efeitos adversos , Polimorfismo de Nucleotídeo Único , Subfamília B de Transportador de Cassetes de Ligação de ATP/genética , Adolescente , Adulto , Grupo com Ancestrais do Continente Asiático/genética , Grupo com Ancestrais do Continente Asiático/estatística & dados numéricos , Estudos de Casos e Controles , Feminino , Necrose da Cabeça do Fêmur/epidemiologia , Estudos de Associação Genética , Predisposição Genética para Doença , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem
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