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1.
J Comput Assist Tomogr ; 44(1): 102-110, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31939890

RESUMO

OBJECTIVE: To evaluate the overall diagnostic value of magnetic resonance imaging (MRI) in restaging of rectal cancer after preoperative chemoradiotherapy based on qualified studies. METHODS: PubMed, Cochrane, and EMBASE database were searched by the index words to identify the qualified studies, and relevant literature sources were also searched. The latest research was done in April 2019. Heterogeneity of the included studies was tested, which was used to select proper effect model to calculate pooled weighted sensitivity, specificity, and diagnostic odds ratio (DOR). Summary receiver operating characteristic (SROC) analyses were also performed. RESULT: Nineteen studies with 1262 patients were involved in the meta-analysis exploring the diagnostic accuracy of MRI for rectal cancer. The diagnostic accuracy of MRI in T3-T4 rectal cancer was as follows: sensitivity, 81% (95% confidence interval [CI], 67%-90%); specificity, 67% (95% CI, 51%-80%); positive likelihood ratio, 2.48 (95% CI, 1.57-3.91); negative likelihood ratio, 0.28 (95% CI, 0.15-0.52); global DOR, 6.86 (95% CI, 3.07-15.30); the area under the SROC was high (0.81; 95% CI, 0.78-0.84). The diagnostic accuracy of MRI in lymphatic metastasis of rectal cancer was as follows: sensitivity, 77% (95% CI, 65%-86%); specificity, 77% (95% CI, 63%-87%); positive likelihood ratio, 3.40 (95% CI, 2.07-5.59); negative likelihood ratio, 0.30 (95% CI, 0.20-0.45); DOR, 10.81 (95% CI, 4.99-23.39); area under the SROC was high (0.84; 95% CI, 0.80-0.87). CONCLUSIONS: This study provides a systematic review and meta-analysis of diagnostic accuracy studies of MRI for rectal cancer. The results indicate that MRI is a highly accurate diagnostic tool for rectal cancer T3-T4 staging and N staging but sensitivity and specificity are not high.


Assuntos
Quimiorradioterapia/métodos , Imagem por Ressonância Magnética/métodos , Neoplasias Retais/diagnóstico por imagem , Neoplasias Retais/terapia , Humanos , Metástase Linfática , Terapia Neoadjuvante , Estadiamento de Neoplasias , Razão de Chances , Curva ROC , Neoplasias Retais/patologia , Sensibilidade e Especificidade , Resultado do Tratamento
2.
R Soc Open Sci ; 6(6): 190150, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31312486

RESUMO

Andrographis paniculata is an important traditional medicinal herb in South and Southeast Asian countries with diverse pharmacological activities that contains various flavonoids and flavonoid glycosides. Glycosylation can transform aglycones into more stable, biologically active and structurally diverse glycosides. Here, we report three glycosyltransferases from the leaves of A. paniculata (ApUFGTs) that presented wide substrate spectra for flavonoid glycosylation and exhibited multi-site glycosylation on the substrate molecules. They acted on the 7-OH position of the A ring and were able to glycosylate several other different types of compounds. The biochemical properties and phylogenetic analysis of these glycosyltransferases were also investigated. This study provides a basis for further research on the cloning of genes involved in glycosylation from A. paniculata and offers opportunities for enhancing flavonoid glycoside production in heterologous hosts. These enzymes are expected to become effective tools for drug discovery and for the biosynthesis of derivatives via flavonoid glycosylation.

3.
Mol Med Rep ; 19(5): 4484-4490, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30896807

RESUMO

Charcot­Marie­Tooth (CMT) disease is a group of motor and sensory neuropathies with a high degree of pathological and genetic heterogenicity. The present study described 2 patients with CMT in a Chinese Han pedigree. The proband exhibited the classic manifestation of CMT with slowly progressing muscular atrophy and weakness. Electrophysiological examination highlighted axonal and demyelinating features. His mother did not have any symptoms, but did exhibit abnormal electrophysiological results. Next­generation sequencing technology was employed to screen mutations in the genes associated with inherited motor never diseases. A novel mutation, c.528_530delAGT, in the gap junction protein beta 1 (GJB1) gene for CMTX, and a rare variation, c.2369C>T, in the dehydrogenase E1 and transketolase domain containing 1 (DHTKD1) gene for CMT disease type 2Q (CMT2Q), were identified in the proband and his mother. The results were verified by Sanger sequencing. Although the in silico analysis predicted no change in the 3­dimensional structure, the clinical and electrophysiological presentation in the pedigree and the high evolutionary conservation of the affected amino acid supported the hypothesis that the c.528_530delAGT mutation in the GJB1 gene may be pathogenic in this pedigree. In silico analysis and high evolutionary conservation suggested the pathogenicity of the c.2369C>T mutation in the DHTKD1 gene; however, the clinical and electrophysiological performances of the proband and his mother did not conform to those of CMT2Q caused by the DHTKD1 gene. The present study provided additional information concerning the range of mutations of the GJB1 gene, which facilitated the understanding of the genotype­phenotype association of CMT.


Assuntos
Grupo com Ancestrais do Continente Asiático/genética , Doença de Charcot-Marie-Tooth/patologia , Conexinas/genética , Cetona Oxirredutases/genética , Adulto , Doença de Charcot-Marie-Tooth/genética , China , Conexinas/química , Análise Mutacional de DNA , Eletromiografia , Deleção de Genes , Humanos , Cetona Oxirredutases/química , Masculino , Pessoa de Meia-Idade , Linhagem , Polimorfismo de Nucleotídeo Único , Estrutura Terciária de Proteína
4.
Clin Transl Gastroenterol ; 9(11): 204, 2018 11 12.
Artigo em Inglês | MEDLINE | ID: mdl-30420730

RESUMO

OBJECTIVES: Rare pathogenic variants in the SPINK1, PRSS1, CTRC, and CFTR genes have been strongly associated with a risk of developing chronic pancreatitis (CP). However, their potential impact on the age of disease onset and clinical outcomes, as well as their potential interactions with environmental risk factors, remain unclear. These issues are addressed here in a large Chinese CP cohort. METHODS: We performed targeted next-generation sequencing of the four CP-associated genes in 1061 Han Chinese CP patients and 1196 controls. To evaluate gene-environment interactions, the patients were divided into three subgroups, idiopathic CP (ICP; n = 715), alcoholic CP (ACP; n = 206), and smoking-associated CP (SCP; n = 140). The potential impact of rare pathogenic variants on the age of onset of CP and clinical outcomes was evaluated using the Kaplan-Meier model. RESULTS: We identified rare pathogenic genotypes involving the SPINK1, PRSS1, CTRC, and/or CFTR genes in 535 (50.42%) CP patients but in only 71 (5.94%) controls (odds ratio = 16.12; P < 0.001). Mutation-positive patients had significantly earlier median ages at disease onset and at diagnosis of pancreatic stones, diabetes mellitus and steatorrhea than mutation-negative ICP patients. Pathogenic genotypes were present in 57.1, 39.8, and 32.1% of the ICP, ACP, and SCP patients, respectively, and influenced age at disease onset and clinical outcomes in all subgroups. CONCLUSIONS: We provide evidence that rare pathogenic variants in the SPINK1, PRSS1, CTRC, and CFTR genes significantly influence the age of onset and clinical outcomes of CP. Extensive gene-environment interactions were also identified.


Assuntos
Idade de Início , Interação Gene-Ambiente , Genótipo , Pancreatite Crônica/genética , Adolescente , Adulto , Grupo com Ancestrais do Continente Asiático/genética , Cálculos/diagnóstico , Quimotripsina/genética , Regulador de Condutância Transmembrana em Fibrose Cística/genética , Diabetes Mellitus/diagnóstico , Predisposição Genética para Doença , Humanos , Estimativa de Kaplan-Meier , Pessoa de Meia-Idade , Mutação , Pancreatopatias/diagnóstico , Pancreatite Alcoólica/genética , Pancreatite Crônica/diagnóstico , Fumar/efeitos adversos , Esteatorreia/diagnóstico , Tripsina/genética , Inibidor da Tripsina Pancreática de Kazal/genética , Adulto Jovem
5.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 40(5): 705-709, 2018 Oct 30.
Artigo em Chinês | MEDLINE | ID: mdl-30404706

RESUMO

The development and metastasis of uterine tumors depend highly on tumor angiogenesis. Multiphase dynamic contrast-enhanced magnetic resonance imaging can quantitatively describe the hemodynamic changes of uterine tumors based on a variety of tracer kinetic models and time-signal curves and by simulating the distribution of contrast inside and outside the blood vessels. Functional parameters can accurately and noninvasively assess tumor angiogenesis. It provides a non-invasive functional evaluation method for the differential diagnosis,staging,response evaluation,and prognostic prediction of uterine tumors.


Assuntos
Imagem por Ressonância Magnética , Neovascularização Patológica/diagnóstico por imagem , Neoplasias Uterinas/diagnóstico por imagem , Meios de Contraste , Feminino , Humanos , Perfusão
6.
Huan Jing Ke Xue ; 39(5): 2104-2116, 2018 May 08.
Artigo em Chinês | MEDLINE | ID: mdl-29965510

RESUMO

Fluorescent substances are used as good tracers in dissolved organic matter (DOM) to identify the source of DOM and its geochemical behavior in a hydrological system. However, there are few studies on the karst aquifer system. Many parameters in karst systems affect the DOM spectral information. A typical karst watershed in Northern China was selected in this research. Excitation-emission matrices (EEMs), parallel factor analysis (PARAFAC), and hydrochemical data were applied to reveal the relationship between the composition and transformation of DOM fluorescent substances in different karst water-bearing spaces. The source of DOM and the effect of water chemistry on DOM transfer were also discussed. The results showed that DOM in exogenous surface water and karst surface water in the Yufu River watershed were mainly composed of tryptophan-like substances, while the DOM in shallow karst water and deep karst water consisted of tryptophan-like and tyrosine-like substances. A comprehensive analysis by fluorescence index (FI), biological index (BIX), and humification index (HIX) displayed that the DOM in shallow and deep karst water resulted from microbial decomposition. In contrast, the DOM in karst surface water and exogenous surface water resulted from land-based input and endogenous microbial decomposition, in which endogenous contributions occupy a large proportion. Due to the chemical parameters of karst water, these three kinds of fluorescent substances extracted by PARAFAC had obviously different characteristics, i.e., ① the tyrosine-like substances had a strong adaptability to Ca2+ and HCO3-, and the proportion of the tyrosine in karst water was relatively large; ② the tryptophan substance followed an opposite trend; and ③ there was a significant positive correlation between fulvic acid and TDS, turbidity, Cl-, and SO42-. Observations of the watershed runoff revealed that the DOM in shallow karst water in the upper reaches came mainly from the soil and microbial degradation. The organic matter underwent a large amount of microbial decomposition and exogenous input when the water was rejuvenated with springs. After infiltration to the deep karst water in the lower reaches, the DOM gradually were converted to low aromatic hydrocarbon organic compounds and decreased macromolecules of DOM. Subsequently, the fluorescence intensity was weakened. The principal component analysis (PCA) extracted three principal components. They were the water mineralization index, soil leaching index, and hydrochemical/biochemical process index. The water mineralization index consists of hydrochemical parameters reflecting the water infiltration, transformation, and flow conditions in the karst system. The soil leaching index contains TOC, NO3-, and protein-like indicators relating to the relationship between protein-like substances and soil and natural leaching. The hydrochemical/biochemical process index is composed of Ca2+, HCO3-, FI, and fulvic acid indicators that illustrate the water chemistry and biochemical processes in the karst water system. In addition, the study also showed that total fluorescence intensity, fulvate-like substances, and protein-like substances can be used as a tracer for rapid seepage, transformation, and aquifer fragility for karst water, respectively. The results of the study are important in understanding the biogeochemical cycle of DOM in the karst water system and also helpful for controlling organic pollution. It also provides a new tool for characterizing the geochemical processes of organic matter in karst system.

7.
Front Aging Neurosci ; 10: 438, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30692923

RESUMO

The diagnosis of PD might be in difficulty, especially in the early stages. Therefore, the identification of novel biomarkers is imperative for the diagnosis and monitoring disease progression in PD. DJ-1 and α-synuclein, are two proteins that are critically involved in the pathogenesis of PD, and they have been examined as disease biomarkers in studies. However, no study exists regarding DJ-1 in plasma neural-derived exosomes. In the present study, the levels of DJ-1 and α-synuclein in plasma neural-derived exosomes were studied together in order to investigate novel biomarkers for PD. DJ-1 and α-synuclein in plasma and plasma neural-derived exosomes of the patients with PD and controls were quantified by ELISAs. The data revealed that the levels of DJ-1 and α-synuclein in plasma neural-derived exosomes and the ratio of plasma neural-derived exosomal DJ-1 to total DJ-1 were significantly higher in patients with PD, compared with controls, while levels of the two proteins in plasma exhibited no difference between the patients with PD and controls. However, no relationship was identified between biomarkers and disease progression. In addition, significant positive correlations between DJ-1 and α-synuclein in plasma neural-derived exosomes were found in the patients with PD and in healthy individuals. We hypothesize that DJ-1 in plasma neural-derived exosomes may be used as a potential biomarker as α-synuclein in PD and they might participate in the mechanism of PD together.

8.
Gastrointest Endosc ; 87(1): 321-322, 2018 01.
Artigo em Inglês | MEDLINE | ID: mdl-29241861
9.
Exp Ther Med ; 14(3): 2241-2248, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28962149

RESUMO

Endothelial dysfunction, regarded as a key step in the pathophysiological course of diabetic vascular complications, is initiated and deteriorated by advanced glycation end products (AGEs). DL-3-n-butylphthalide (DL-NBP) has been proven to have protective effects on neurons and vascular endothelial cells against ischemic and anoxic damage. The aim of the present study was to investigate whether NBP is able to attenuate AGE-induced endothelial dysfunction in vitro, and also elucidate the possible underlying mechanism. An injury model of human umbilical vein endothelial cells (HUVECs) induced by AGEs (200 µg/ml) was established. The results demonstrated that pretreatment with NBP (1-100 µM) significantly increased HUVEC viability and inhibited the apoptosis induced by AGEs. In addition, AGEs stimulated the expression levels of the receptor for AGEs protein and the downstream protein nuclear factor-κB in HUVECs, which were inhibited by pretreatment with NBP. Furthermore, it significantly reduced reactive oxygen species generation and the level of the inflammatory cytokines, intercellular cell adhesion molecule-1 and monocyte chemotactic protein-1, in HUVECs mediated by AGEs. The current findings indicated that NBP attenuated AGE-induced endothelial dysfunction by ameliorating inflammation and oxidative stress responses.

10.
Hum Mutat ; 38(8): 959-963, 2017 08.
Artigo em Inglês | MEDLINE | ID: mdl-28497564

RESUMO

Rare functionally defective carboxypeptidase A1 (CPA1) variants have been reported to predispose to nonalcoholic chronic pancreatitis, mainly the idiopathic subtype. However, independent replication has so far been lacking, particularly in Asian cohorts where initial studies employed small sample sizes. Herein we performed targeted next-generation sequencing of the CPA1 gene in 1,112 Han Chinese idiopathic chronic pancreatitis (ICP) patients-the largest ICP cohort so far analyzed in a single population-and 1,580 controls. Sanger sequencing was used to validate called variants, and the CPA1 activity and secretion of all newly found variants were measured. A total of 18 rare CPA1 variants were characterized, 11 of which have not been previously described. However, no significant association was noted with ICP irrespective of whether all rare variants [20 out of 1,112 (1.8%) in patients vs. 24 out of 1,580 (1.52%) in controls; P = 0.57] or functionally impaired variants [three out of 1,112 (0.27%) in patients vs. two out of 1,580 (0.13%) in controls; P = 0.68] were considered.


Assuntos
Carboxipeptidases A/metabolismo , Pancreatite Crônica/enzimologia , Grupo com Ancestrais do Continente Asiático , Carboxipeptidases A/genética , Estudos de Coortes , Feminino , Predisposição Genética para Doença/genética , Genótipo , Humanos , Masculino , Mutação/genética , Pancreatite Crônica/genética
11.
J Geriatr Cardiol ; 14(1): 49-56, 2017 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-28270842

RESUMO

OBJECTIVES: Inflammation has been shown to be related with acute aortic dissection (AAD). The present study aimed to evaluate the association of white blood cell counts (WBCc) on admission with both in-hospital and long-term all-cause mortality in patients with uncomplicated Stanford type B AAD. METHODS: From 2008 to 2010, a total of 377 consecutive patients with uncomplicated type B AAD were enrolled and then followed up. Clinical data and WBCc on admission were collected. The primary end points were in-hospital death and long-term all-cause death. RESULTS: The in-hospital death rate was 4.2%, and the long-term all-cause mortality rate was 6.9% during a median follow-up of 18.9 months. WBCc on admission was identified as a risk factor for in-hospital death by univariate Cox regression analysis as both a continuous variable and a categorical variable using a cut off of 11.0 × 109 cell/L (all P < 0.05). After adjusting for age, sex and other risk factors, elevated admission WBCc was still a significant predictor for in-hospital death as both a continuous variable [hazard ratio (HR): 1.052, 95% CI: 1.024-1.336, P = 0.002] and a categorical variable using a cut off of 11.0 × 109 cell/L (HR: 2.056, 95% CI: 1.673-5.253, P = 0.034). No relationship was observed between WBCc on admission and long-term all-cause death. CONCLUSIONS: Our results indicate that elevated WBCc upon admission might be used as a predictor for increased risk of in-hospital death in uncomplicated type B AAD. There might be no predictive value of WBCc for the long-term survival of type B AAD.

12.
Gastrointest Endosc ; 86(3): 519-524.e1, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28062312

RESUMO

BACKGROUND AND AIMS: Almost all studies on post-ERCP pancreatitis (PEP) have mainly involved patients with biliary diseases rather than chronic pancreatitis (CP), and the concept that CP seems to be a protective factor associated with PEP has not been studied in detail. The aim of this study was to determine the incidence of PEP in patients with CP at different clinical stages and to identify the predictive and protective factors of PEP in a large cohort. METHODS: In this observational cohort study, medical records of patients with CP (CP group) and biliary diseases (BD group) in a tertiary hospital from January 2011 to May 2015 were examined. The difference in the incidence of PEP between CP group and BD group and the risk of PEP at different clinical stages of CP were calculated by the χ2 test or the Fisher exact test. The predictive and protective factors for PEP were investigated by univariate and multivariate analysis. RESULTS: In total, 2028 ERCP procedures were performed in 1301 patients with CP and 2000 procedures in 1655 patients with BD. The overall incidence of PEP in CP group (4.5%) was similar to that in the BD group (4.8%; P = .747). However, CP patients had significantly lower rates of moderate and severe attacks (0% vs 1.3%, P < .01). According to the M-ANNHEIM classification, the PEP incidences of CP at stages 0, I, II, III, and IV were 4.4%, 5.1%, 3.8%, 2.0%, and 2.0%, respectively. CP patients at stage Ia had the highest PEP incidence (8.0%) among all CP patients, significantly higher than that at stages Ib + Ic (3.9%) and II (3.8%). Female gender, history of acute pancreatitis, and prior PEP were independent risk factors of PEP, whereas extracorporeal shock wave lithotripsy was a protective factor. CONCLUSIONS: Compared with BD patients, CP patients had similar incidence of PEP overall but lower grades of severity. The incidence of PEP in CP patients decreased significantly with disease progression. (Clinical trial registration number: NCT02781987.).


Assuntos
Doenças Biliares/cirurgia , Colangiopancreatografia Retrógrada Endoscópica , Pancreatopatias/cirurgia , Pancreatite/epidemiologia , Complicações Pós-Operatórias/epidemiologia , Doença Aguda , Adulto , Idoso , China/epidemiologia , Estudos de Coortes , Constrição Patológica/cirurgia , Feminino , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Ductos Pancreáticos , Pancreatite Crônica , Fatores de Proteção , Estudos Retrospectivos , Fatores de Risco , Índice de Gravidade de Doença , Fatores Sexuais
13.
Medicine (Baltimore) ; 96(52): e8967, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29384895

RESUMO

RATIONALE: Plexiform fibromyxoma (PF) is an extremely rare mesenchymal tumor of the stomach, and its radiological findings have not been well described. Here, we analyzed the imaging features of a case of PF. To our knowledge, this is a rare reported case with a remarkable cystic change in the imaging literature. PATIENT CONCERNS: A previously healthy 50-year-old woman presented with a 1-day history of abdominal pain. Then, she underwent computed tomography (CT) and magnetic resonance imaging (MRI). A cystic-solid well-circumscribed extraluminal mass was located in the posterior wall of the gastric upper body. The solid portion appeared as heterogeneous attenuation/intensity with progressive enhancement while the cystic region had no enhancement. DIAGNOSES: The potential for malignancy could not be excluded. INTERVENTIONS: Laparoscopic partial gastric resection was performed. OUTCOMES: Based on pathological findings, a diagnosis of PF was made. The patient was alive without any recurrence or metastasis of the tumor after 2 years of follow-up. LESSONS: As far as we know, a gastric PF with a remarkable cystic change has never been reported. Additionally, the tumor exhibited a progressive enhancement pattern which is a characteristic radiographic feature in our case. Our report may help increase the awareness of this rare but important new disease entity.


Assuntos
Fibroma/diagnóstico por imagem , Neoplasias Gástricas/diagnóstico por imagem , Feminino , Fibroma/patologia , Fibroma/cirurgia , Gastroscopia , Humanos , Imagem por Ressonância Magnética , Pessoa de Meia-Idade , Neoplasias Gástricas/patologia , Neoplasias Gástricas/cirurgia , Tomografia Computadorizada por Raios X
14.
Rep Prog Phys ; 79(7): 076201, 2016 07.
Artigo em Inglês | MEDLINE | ID: mdl-27325301

RESUMO

Behind the observed pattern of lepton flavor mixing is a partial or approximate µ-τ flavor symmetry-a milestone on our road to the true origin of neutrino masses and flavor structures. In this review article we first describe the features of µ-τ permutation and reflection symmetries, and then explore their various consequences on model building and neutrino phenomenology. We pay particular attention to soft µ-τ symmetry breaking, which is crucial for our deeper understanding of the fine effects of flavor mixing and CP violation.

15.
World J Gastroenterol ; 22(13): 3652-62, 2016 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-27053857

RESUMO

AIM: To investigate the feasibility of a dual-input two-compartment tracer kinetic model for evaluating tumorous microvascular properties in advanced hepatocellular carcinoma (HCC). METHODS: From January 2014 to April 2015, we prospectively measured and analyzed pharmacokinetic parameters [transfer constant (Ktrans), plasma flow (Fp), permeability surface area product (PS), efflux rate constant (kep), extravascular extracellular space volume ratio (ve), blood plasma volume ratio (vp), and hepatic perfusion index (HPI)] using dual-input two-compartment tracer kinetic models [a dual-input extended Tofts model and a dual-input 2-compartment exchange model (2CXM)] in 28 consecutive HCC patients. A well-known consensus that HCC is a hypervascular tumor supplied by the hepatic artery and the portal vein was used as a reference standard. A paired Student's t-test and a nonparametric paired Wilcoxon rank sum test were used to compare the equivalent pharmacokinetic parameters derived from the two models, and Pearson correlation analysis was also applied to observe the correlations among all equivalent parameters. The tumor size and pharmacokinetic parameters were tested by Pearson correlation analysis, while correlations among stage, tumor size and all pharmacokinetic parameters were assessed by Spearman correlation analysis. RESULTS: The Fp value was greater than the PS value (FP = 1.07 mL/mL per minute, PS = 0.19 mL/mL per minute) in the dual-input 2CXM; HPI was 0.66 and 0.63 in the dual-input extended Tofts model and the dual-input 2CXM, respectively. There were no significant differences in the kep, vp, or HPI between the dual-input extended Tofts model and the dual-input 2CXM (P = 0.524, 0.569, and 0.622, respectively). All equivalent pharmacokinetic parameters, except for ve, were correlated in the two dual-input two-compartment pharmacokinetic models; both Fp and PS in the dual-input 2CXM were correlated with Ktrans derived from the dual-input extended Tofts model (P = 0.002, r = 0.566; P = 0.002, r = 0.570); kep, vp, and HPI between the two kinetic models were positively correlated (P = 0.001, r = 0.594; P = 0.0001, r = 0.686; P = 0.04, r = 0.391, respectively). In the dual input extended Tofts model, ve was significantly less than that in the dual input 2CXM (P = 0.004), and no significant correlation was seen between the two tracer kinetic models (P = 0.156, r = 0.276). Neither tumor size nor tumor stage was significantly correlated with any of the pharmacokinetic parameters obtained from the two models (P > 0.05). CONCLUSION: A dual-input two-compartment pharmacokinetic model (a dual-input extended Tofts model and a dual-input 2CXM) can be used in assessing the microvascular physiopathological properties before the treatment of advanced HCC. The dual-input extended Tofts model may be more stable in measuring the ve; however, the dual-input 2CXM may be more detailed and accurate in measuring microvascular permeability.


Assuntos
Carcinoma Hepatocelular/diagnóstico por imagem , Meios de Contraste/farmacocinética , Neoplasias Hepáticas/diagnóstico por imagem , Imagem por Ressonância Magnética , Microvasos/diagnóstico por imagem , Modelos Biológicos , Idoso , Permeabilidade Capilar , Carcinoma Hepatocelular/irrigação sanguínea , Carcinoma Hepatocelular/metabolismo , Meios de Contraste/administração & dosagem , Estudos de Viabilidade , Feminino , Humanos , Circulação Hepática , Neoplasias Hepáticas/irrigação sanguínea , Neoplasias Hepáticas/metabolismo , Masculino , Microcirculação , Microvasos/fisiopatologia , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Estudos Prospectivos , Fluxo Sanguíneo Regional , Carga Tumoral
16.
J Neurol Sci ; 364: 19-23, 2016 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-27084208

RESUMO

OBJECTIVE: To explore the risk factors associated with acute/subacute cerebral infarction (ASCI) in HIV-negative patients with cryptococcal meningitis (CM). METHODS: This case-control study included 10 HIV-negative CM patients with ASCI and 30 age- and sex-matched HIV-negative control (1:3) CM patients without ASCI. The clinical manifestations and neuroimaging findings were collected. Risk factors for ASCI in the HIV-negative CM patients were confirmed by conditional logistic regression analysis. RESULTS: Among the 10 HIV-negative CM patients with ASCI, all cases had lacunar infarctions. Single infarctions were found in 6 patients, and multiple infarctions in 4. Hydrocephalus (p=0.020, OR=23.77, 95% CI, 1.67-339.33) and smoking (p=0.039, OR=11.63, 95% CI, 1.14-118.96) were found to be independently associated with the occurrence of ASCI. CONCLUSIONS: Hydrocephalus and smoking may increase the risk of ASCI in HIV-negative CM patients. In the clinical course, cerebral infarction should be strongly suspected in CM patients with hydrocephalus or smoking histories.


Assuntos
Infarto Cerebral/etiologia , Meningite Criptocócica/complicações , Fatores de Risco , Adolescente , Adulto , Idoso , Estudos de Casos e Controles , Infarto Cerebral/diagnóstico por imagem , Imagem de Difusão por Ressonância Magnética , Feminino , Humanos , Hidrocefalia/etiologia , Masculino , Meningite Criptocócica/diagnóstico por imagem , Pessoa de Meia-Idade , Estudos Retrospectivos , Estatísticas não Paramétricas , Adulto Jovem
17.
Int J Clin Exp Pathol ; 8(2): 1954-60, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25973088

RESUMO

INTRODUCTION: long non-coding RNAs (lncRNAs) are emerging as new regulators in the cancer paradigm, the involvement of lncRNAs in urothelial carcinoma of the bladder (UCB) is just beginning to be studied. In this study, we focused on lncRNA SPRY4-IT1 and investigated its expression pattern, clinical significance, and biological function in UCB. METHODS: SPRY4-IT1 expression in UCB tissues was examined by quantitative Real-time PCR (qRT-PCR) and its correlation with clinicopathological features and patient prognosis was later analyzed. Moreover, in vitro assays were performed to explore its role in bladder cancer progression. RESULTS: SPRY4-IT1 expression was elevated in UCB tissues, and SPRY4-IT1 levels were highly positively correlated with histological grade, tumor stage, and lymph node metastasis and reduced overall survival. A multivariate analysis showed that SPRY4-IT1 expression is an independent prognostic factor of overall survival in patients with UCB. Additionally, the results of in vitro assays showed that the suppression of SPRY4-IT1 expression in bladder cancer cells significantly inhibit cell proliferation, migration, and invasion. CONCLUSIONS: Our data suggested that lncRNA SPRY4-IT1 is a novel molecule involved in bladder cancer progression, which provide a potential prognostic biomarker and therapeutic target.


Assuntos
Proliferação de Células , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Invasividade Neoplásica/genética , Metástase Neoplásica/genética , Proteínas do Tecido Nervoso/metabolismo , RNA Longo não Codificante , Neoplasias da Bexiga Urinária/metabolismo , Biomarcadores Tumorais/genética , Biomarcadores Tumorais/metabolismo , Linhagem Celular Tumoral , Movimento Celular , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Peptídeos e Proteínas de Sinalização Intracelular/genética , Masculino , Pessoa de Meia-Idade , Invasividade Neoplásica/patologia , Metástase Neoplásica/patologia , Proteínas do Tecido Nervoso/genética , Prognóstico , Regulação para Cima , Neoplasias da Bexiga Urinária/genética , Neoplasias da Bexiga Urinária/patologia
18.
Mol Med Rep ; 11(2): 1235-9, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25370074

RESUMO

The aim of the current study was to identify the genes on human chromosome 21 (HC21) that may serve important functions in the pathogenesis of Down syndrome (DS). The microarray data GSE5390 were obtained from the Gene Expression Omnibus database, which contained 7 DS and 8 healthy normal samples. The data were then normalized and the differentially expressed genes (DEGs) were identified using the LIMMA package and Bonferroni correction. Furthermore, the DEGs underwent clustering and gene ontology analysis. Additionally, the locations of the DEGs on HC21 were confirmed using human genome 19 in the University of California, Santa Cruz Interaction Browser. A total of 25 upregulated and 275 downregulated genes were screened between DS and healthy samples with a false discovery rate of <0.05 and |logFC|>1. The expression levels of these genes in the two samples were different. In addition, the up­ and downregulated genes were markedly enriched in organic substance biological processes (P=4.48x10­10) and cell­cell signaling (P=0.000227). Furthermore, 17 overexpressed genes were identified on the 21q21­22 area, including COL6A2, TTC3 and ABCG1. Together, these observations suggest that 17 upregulated genes on HC21 may be involved in the development of DS and provide the basis for understanding this disability.


Assuntos
Cromossomos Humanos Par 21/genética , Síndrome de Down/genética , Córtex Pré-Frontal/metabolismo , Cromossomos Humanos Par 21/metabolismo , Análise por Conglomerados , Biologia Computacional , Bases de Dados Genéticas , Síndrome de Down/patologia , Regulação para Baixo , Humanos , Análise de Sequência com Séries de Oligonucleotídeos , Regulação para Cima
19.
World J Gastroenterol ; 20(29): 10202-7, 2014 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-25110449

RESUMO

Primary gastric plasmacytoma (GP) is a rare extramedullary plasmacytoma with clinical and imaging features that are common among other gastric tumors, such as gastric adenocarcinomas, gastric stromal tumors, and lymphomas. Here, we present a histologically confirmed case of primary GP examined with biphasic computed tomography (CT), magnetic resonance imaging (MRI), and endosonography. A well-circumscribed extraluminal mass appearing as homogeneous attenuation/intensity with gradual enhancement was identified on biphasic enhancement CT and MRI. This mass was hyperintense on diffusion-weighted imaging and hypointense on the apparent diffusion coefficient map, implying that water diffusion in the mass was restricted. In addition, endosonography indicated a low echogenic mass in the gastric wall. These imaging findings increase the available knowledge about imaging of this disease and provide valuable information for differentiating primary GP from common gastric tumors.


Assuntos
Diagnóstico por Imagem/métodos , Plasmocitoma/diagnóstico , Neoplasias Gástricas/diagnóstico , Idoso , Biomarcadores Tumorais/análise , Biópsia , Endossonografia , Gastroscopia , Humanos , Imuno-Histoquímica , Imagem por Ressonância Magnética , Masculino , Imagem Multimodal , Plasmocitoma/química , Plasmocitoma/diagnóstico por imagem , Plasmocitoma/patologia , Plasmocitoma/cirurgia , Valor Preditivo dos Testes , Neoplasias Gástricas/química , Neoplasias Gástricas/diagnóstico por imagem , Neoplasias Gástricas/patologia , Neoplasias Gástricas/cirurgia , Tomografia Computadorizada por Raios X , Resultado do Tratamento
20.
Artigo em Chinês | MEDLINE | ID: mdl-23189834

RESUMO

OBJECTIVE: The aim of this study is to investigate the possible associations of chemokines IP-10, Rantes and oxidative stress in chronic hepatits B (CHB). METHODS: 70 CHB patients and 10 healthy controls were enrolled in the study. Enzyme-linked immunosorbent assay (ELISA) was used to detect the serum levels of IFN-gamma-inducible protein-10 (IP-10) and regulated on activation normal T-cell-expressed and secreted (Rantes) and oxidative stress parameters (glutathione, GSH; glutathione disulfide, GSSG). Correlationship were analyzed by Spearman's rank correlation. RESULT: The levels of IP-10 and Rantes were higher in CHB patients than healthy controls, and strong positive associations were found between IP-10/Rantes and alanine aminotransferase (ALT). The levels of GSH and GSH/GSSG were lower in CHB patients than healthy controls, and GSH and GSH/GSSG were negatively correlated with ALT. The levels of IP-10 and Rantes were negatively correlated with GSH and GSH/GSSG respectively. CONCLUSION: Strong associations were found between chemokines and oxidative stress which participated in the pathogenesis of CHB.


Assuntos
Quimiocina CCL5/sangue , Quimiocina CXCL10/sangue , Hepatite B Crônica/metabolismo , Estresse Oxidativo , Adulto , Alanina Transaminase/sangue , Feminino , Glutationa/sangue , Dissulfeto de Glutationa/sangue , Vírus da Hepatite B/genética , Vírus da Hepatite B/isolamento & purificação , Hepatite B Crônica/sangue , Hepatite B Crônica/virologia , Humanos , Masculino , Malondialdeído/sangue , Pessoa de Meia-Idade
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