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1.
Org Lett ; 2020 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-32223202

RESUMO

Herein, we describe a gold(I)-catalyzed generation of nonclassical gold-containing 1,4-all-carbon dipoles from cycloisomerization/1,2-carbene transfer/ring opening cascade reactions of readily accessible allenyl ketones bearing a cyclopropyl moiety and its cyclization with tropone. This method features an unprecedented formal [8+4] high-order cycloaddition under mild conditions for delivering structurally complex 7,7,5-tricycles in generally moderate to high yields.

2.
Sci Total Environ ; 723: 137990, 2020 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-32203800

RESUMO

Bioelectrochemical system (BESs) has been applied to treat refractory wastewaters such as phenolic wastewater since microbial anodic oxidation driven by electroactive bacteria is believed to enhance decomposition of organic matters. Considering that most of electroactive bacteria are sensitive to phenol and cannot utilize it directly, it was assumed that fermentative bacteria and electroactive bacteria in mixed-culture BESs cooperated to degrade phenol. To clarify this assumption, a microbial electrolysis cell (MEC) for phenol degradation with Cr(VI)-reduction bio-cathode was developed in this study. Results showed that phenol served as anodic electron donor was more efficient than acetate for cathodic reduction of Cr(VI) since the slow release of acetate from phenol degradation with fermentative bacteria might make full use of acetate as electron donor for anodic oxidation. The production of quorum sensing (QS) signal molecules were higher in phenolic anolyte, confirming the syntrophic metabolism among phenol-degrading bacteria and electroactive bacteria. Cyclic voltammetry (CV) test and Fourier transform infrared spectroscopy (FT-IR) indicated that phenolic anolyte and anodic sludge had higher electron transfer ability due to enhanced bio-electrochemisty processes in syntrophic metabolism.

3.
Artigo em Inglês | MEDLINE | ID: mdl-32050535

RESUMO

Bayesian parameter inversion approaches are dependent on the original forward models linking subsurface physical properties to measured data, which usually require a large number of iterations. Fast alternative systems to forward models are commonly employed to make the stochastic inversion problem computationally tractable. This paper compared the effect of the original forward model constructed by the HYDRUS-1D software and two different approximations: the Artificial Neural Network (ANN) alternative system and the Gaussian Process (GP) surrogate system. The model error of the ANN was quantified using a principal component analysis, while the model error of the GP was measured using its own variance. There were two groups of measured pressure head data of undisturbed loess for parameter inversion: one group was obtained from a laboratory soil column infiltration experiment and the other was derived from a field irrigation experiment. Strong correlations between the pressure head values simulated by random posterior samples indicated that the approximate forward models are reliable enough to be included in the Bayesian inversion framework. The approximate forward models significantly improved the inversion efficiency by comparing the observed and the optimized results with a similar accuracy. In conclusion, surrogates can be considered when the forward models are strongly nonlinear and the computational costs are prohibitive.

4.
Org Lett ; 22(3): 848-853, 2020 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-31939307

RESUMO

By choosing suitable ligand-directed gold catalysts, two types of gold-containing all-carbon 1,4-dipoles could be generated selectively from the gold(I)-catalyzed cycloisomerizations of allenyl ketones bearing a cyclopropyl moiety, which undergo [4 + 3] cycloadditions with nitrones to produce two regiomers of furan-condensed N,O-seven-membered rings in moderate to excellent yields highly selectively.

5.
Clin Cancer Res ; 2020 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-31937612

RESUMO

PURPOSE: Malignancy of cancer cells depends on the active transcription of tumor-associated genes. Recently, unique clusters of transcriptional enhancers, termed super-enhancers, have been reported to drive the expression of genes that define cell identity. In this study we characterized specific super-enhancer-associated genes of osteosarcoma, and explored their potential therapeutic value. EXPERIMENTAL DESIGN: Super-enhancer regions were characterized through chromatin immunoprecipitation sequencing (ChIP-Seq). RT-qPCR was used to detect the mRNA level of CDK7 in patient specimens and confirm the regulation of sensitive oncogenes by THZ2. The phosphorylation of the initiation-associated sites of RNA polymerase II (RNAP II) C-terminal repeat domain (CTD) was measured using Western blotting. Microarray expression analysis was conducted to explore transcriptional changes after THZ2 treatment. A variety of in vitro and in vivo assays were performed to assess the effects of CDK7 knockdown and THZ2 treatment in osteosarcoma. RESULTS: Super-enhancers were associated with oncogenic transcripts and key genes encoding cell-type-specific transcription factors in osteosarcoma. Knockdown of transcription factor CDK7 reduced phosphorylation of the RNAPII CTD, and suppressed the growth and metastasis of osteosarcoma. A new specific CDK7 inhibitor, THZ2, suppressed cancer biology by inhibition of transcriptional activity. Compared with typical enhancers, osteosarcoma super-enhancer-associated oncogenes were particular vulnerable to this transcriptional disruption. THZ2 exhibited a powerful anti-osteosarcoma effect in vitro and in vivo. Conclusions: Super-enhancer-associated genes contribute to the malignant potential of osteosarcoma, and selectively targeting super-enhancer-associated oncogenes with the specific CDK7 inhibitor THZ2 might be a promising therapeutic strategy for patients with osteosarcoma.

6.
J Orthop Surg Res ; 15(1): 14, 2020 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-31948458

RESUMO

BACKGROUND: Limb salvage for bone sarcoma around the knee in skeletally immature children is challenging because of interference on two critical growth plates in the lower limb. This retrospective study aims to evaluate long-term outcomes and influence on growth of the lower limb of the cemented extendible endoprostheses. METHODS: Forty-five children with bone sarcoma around the knee, who underwent custom-made extendible endoprosthesis replacements, were included in this study. The average follow-up was 10.1 years. Survival, prosthetic-related complications and revision, functional outcomes, and influence on growth by prosthesis implantation were recorded. RESULTS: The 5-year disease-free survival and overall survival are 54.9% and 72.7%, and the 5-year prosthesis survival rate is 59.4%. The prosthesis was extended 4.2 cm in average. Limb length discrepancies of 20 patients were within 2 cm, and growth inhibition of proximal tibial epiphysis by passive implant insertion was observed. Aseptic loosening in 7 patients was the most significant complication. The Musculoskeletal Tumor Society score at last visit was 83.2%. CONCLUSIONS: The use of custom-made extendible endoprosthesis provided good functional results for children with bone tumor around the knee. Further improvement of the prosthesis design and operation technique will help to decrease complication and gain better limb function.

7.
Toxicol Lett ; 319: 138-147, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31730887

RESUMO

Alzheimer's disease (AD) is a neurodegenerative disease that can be induced by heavy metals such as lead. However, there is limited information on the role of blood-brain barrier (BBB) in lead induced AD-like pathology. This study investigates the potential mechanism of lead exposure aggravating the progression of Alzheimer's disease in mice through the BBB. 200 mg/L and 500 mg/L lead acetate were given to C57BL/6J and APP/PS1 mice through drinking water from a week before mating, until the offspring were 7-months-old. 8 female juvenile mice in each group were selected for this investigation. Lead exposure increased blood lead concentration which revealed the internal exposure level, accelerated Aß1-42 deposition in APP/PS1 mouse cortexes and abnormal change in Zonula Occludin-1 (ZO-1) and Claudin-5 protein. It also increased the expression of p-tau in both the C57BL/6J and APP/PS1 mice, and decreased mRNA and protein expression in low-density lipoprotein receptor (LRP-1). Additionally, it increased the mRNA and protein expression of amyloid beta precursor protein (APP) and beta secretase 1 (BACE-1). The activated astrocytes increased in the brains of APP/PS1 mice, and coalesced around the Aß1-42 deposition after lead exposure. The main vessels in deutocerebrum were attached with Aß1-42 deposition. These results offer insight into the mechanism of preventing lead induced AD through cerebrovascular pathways.


Assuntos
Doença de Alzheimer/patologia , Barreira Hematoencefálica/patologia , Exposição Ambiental/efeitos adversos , Chumbo/toxicidade , Doença de Alzheimer/induzido quimicamente , Precursor de Proteína beta-Amiloide/biossíntese , Precursor de Proteína beta-Amiloide/genética , Animais , Barreira Hematoencefálica/efeitos dos fármacos , Córtex Cerebral/efeitos dos fármacos , Córtex Cerebral/metabolismo , Córtex Cerebral/patologia , Claudina-5/efeitos dos fármacos , Claudina-5/genética , Progressão da Doença , Feminino , Chumbo/sangue , Camundongos , Camundongos Endogâmicos C57BL , Compostos Organometálicos/toxicidade , Proteína da Zônula de Oclusão-1/efeitos dos fármacos , Proteína da Zônula de Oclusão-1/genética
8.
Environ Res ; 182: 109045, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31863944

RESUMO

Anaerobic digestion of waste activated sludge (WAS) to produce acetate has recently attracted growing interest, while the slow hydrolytic acidification of sludge and the consumption of acetate by methanogens both decrease the yield of acetate. In this study, Fe3O4 was added to a WAS anaerobic digester under alkaline conditions (pH = 10). The concentration of short-chain fatty acids (SCFA) during WAS anaerobic fermentation was found to be affected positively by Fe3O4. The maximal SCFA production of the Fe3O4-added digester was 3619.4 mg/L, while the maximal SCFA production in the control was 2899.7 mg/L. The increase of SCFA with Fe3O4 was mainly resulted from the increase in acetate accumulation (accounting for 90%), because Fe3O4 stimulated dissimilatory iron reduction (DIR) that participated in the decomposition of complex organics and the transformation of pronionate and butyrate into acetate. Further investigation showed that each step of hydrolytic-acidification process was promoted except the homoacetogenesis. The activity of enzymes and abundance of microbes relevant to hydrolysis and acidification were in agreement with the above results.

9.
Water Res ; 171: 115425, 2020 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-31881499

RESUMO

Stimulating Methanothrix-dominant communities with ethanol is recently considered as a promising strategy of improving the efficiency and stability of anaerobic digestion (AD), while the effects on methanogenic pathway and energy metabolism linked to the establishment of direct interspecies electron transfer (DIET) were not investigated yet. The results showed that, Methanothrix species were the dominant and metabolically active methanogens in the methanogenic sludge fed with the ethanol-type fermentation products, and the abundance of genes that encoded the key enzymes involved in the reduction of carbon dioxide was significantly higher than that fed with the other products, such as propionate and butyrate. Conversely, the abundance of genes that encoded the key enzymes involved in acetate decarboxylation among all the methanogenic sludge were nearly same. In the presence of ethanol, the abundance of gene for pilA significantly increased. The gene for pliA was primarily derived from Sphaerochaeta, Sedimentibacter and Pseudomonas species that were specially abundant and metabolically active. Further analysis showed that, the abundance of genes that encoded V/A-type ATPase in the methanogenic digesters fed with the ethanol-type fermentation products was 1.3-1.5 folds higher than that fed with the other products. As a result, the concentration of total ATP in the cells was increased by 1.8-2.3 folds. These results, and the fact that DIET is the only electron donor to support the reduction of carbon dioxide in Methanothrix species for the first time revealed the mechanisms involved in the establishment of DIET-based methanogenic metabolism with ethanol.


Assuntos
Etanol , Metano , Anaerobiose , Reatores Biológicos , Metagenoma , Methanosarcinaceae
10.
Biomed Res Int ; 2019: 8348430, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31886257

RESUMO

Vascular calcification is an independent risk factor for plaque instability and is associated with endothelial cell function. Here, we investigated the role of endothelial cell function in the calcification of atherosclerotic plaques. We hypothesized that atherosclerosis would be associated with endothelial dysfunction and that bosentan (Tracleer®), a dual endothelin-receptor antagonist, would preserve endothelial cell function in an apolipoprotein E-deficient (ApoE-/-) mouse model of atherosclerosis. Accordingly, 4-6-week-old ApoE-/- mice were fed a high-fat diet and treated with bosentan, and the effects of this treatment on body weight and blood lipid concentrations was evaluated. Endothelial damage in the aortic arch was assessed immunohistochemically to detect the proapoptotic proteins PDCD4, caspase-3, and Bax and the antiapoptotic protein Bcl-2. Notably, bosentan treatment was associated with decreased concentrations of these proteins and of blood lipids in ApoE-/- mice. Consistent with these findings, we observed increased concentrations of miRNA-21 and PDCD4 mRNA expression in the aortic arch endothelium after bosentan treatment. We conclude that bosentan can prevent endothelial cell death and protect against atherosclerosis in ApoE-deficient mice by upregulating miRNA-21.

11.
Sci Rep ; 9(1): 14563, 2019 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-31601927

RESUMO

Research has shown that varying spatial scale through the selection of the total extent of investigation and the grain size of environmental predictor variables has effects on species distribution model (SDM) results and accuracy, but there has been minimal investigation into the interactive effects of extent and grain. To do this, we used a consistently sampled range-wide dataset of giant panda occurrence across southwest China and modeled their habitat and distribution at 4 extents and 7 grain sizes. We found that increasing grain size reduced model accuracy at the smallest extent, but that increasing extent negated this effect. Increasing extent also generally increased model accuracy, but the models built at the second-largest (mountain range) extent were more accurate than those built at the largest, geographic range-wide extent. When predicting habitat suitability in the smallest nested extents (50 km2), we found that the models built at the next-largest extent (500 km2) were more accurate than the smallest-extent models but that further increases in extent resulted in large decreases in accuracy. Overall, this study highlights the impacts of the selection of spatial scale when evaluating species' habitat and distributions, and we suggest more explicit investigations of scale effects in future modeling efforts.

12.
Bioresour Technol ; 291: 121877, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31376672

RESUMO

Bioelectrochemical conversion of CO2 to CH4 is a promising way to increase the calorific value of biogas produced during anaerobic digestion. There are two groups of methanogens enriched in these systems, hydrogenotrophs and acetoclastic methanogens that can also directly accept electrons from an electrode or another microorganism. In this study, a microbial electrolysis cell (MEC) poised at -500 mV (vs. SHE) was operated for biogas upgrading. Methane content in the biogas increased from 71% to >90%, and 8.2% of the CO2 was converted to methane. Methanothrix, an acetoclastic methanogen that can participate in direct electron transfer (DET), and Azonexus, an acetate-oxidizing electrogen, were enriched on the cathode. Transcriptomics revealed that Methanothrix on the cathode were using the CO2 reduction pathway, while Methanothrix in the bulk sludge were using the acetate decarboxylation pathway for production of methane. These results show that stimulation of DET in MEC enhances biogas-upgrading processes.


Assuntos
Biocombustíveis , Methanosarcinaceae , Eletrodos , Eletrólise , Transporte de Elétrons , Elétrons , Metano/metabolismo , Esgotos
13.
Bioresour Technol ; 291: 121858, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31377515

RESUMO

The improved performances during anaerobic degradation of phenol to methane with Fe(OH)3 were usually inapparent, due to its lower solubility (unaccessible to dissimilatory iron reduction) and more positive reduction potential of Fe(III)/Fe(II) (unfavorable for enriching Fe(III)-reducing bacteria [IRBs]). In this study, citrate, the organic chelates, were used to solubilize Fe(III) with the aim of improving the phenol degradation and declining the reduction potential of Fe(III)/Fe(II). Results showed that, in the co-occurrence of citrate and Fe(OH)3, the degradation rates of phenol were about 1.3-fold rapider than that with sole Fe(OH)3. Analysis of cyclic voltammetry demonstrated that the reduction potential of Fe(III)/Fe(II) in the form of Fe(OH)3 (-0.41 to -0.28 V vs Ag/AgCl) declined to -0.61 to -0.41 V. As a result, the Fe(III)-reducing genera, such as Petrimonas and Shewanella, which held a great potential of proceeding syntrophic metabolism via direct interspecies electron transfer (DIET), were significantly enriched.


Assuntos
Quelantes/farmacologia , Compostos Férricos/metabolismo , Ferro/metabolismo , Metano/metabolismo , Fenol/metabolismo , Anaerobiose , Bacteroides/efeitos dos fármacos , Bacteroides/metabolismo , Transporte de Elétrons , Oxirredução , Fenóis/metabolismo , Shewanella/efeitos dos fármacos , Shewanella/metabolismo
14.
Theranostics ; 9(13): 3952-3965, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31281524

RESUMO

Deficiency in homologous recombination repair (HRR) is frequently associated with hormone-responsive cancers, especially the epithelial ovarian cancer (EOC) which shows defects of HRR in up to half of cases. However, whether there are molecular connections between estrogen signaling and HRR deficiency in EOC remains unknown. Methods: We analyzed the estrogen receptor α (ERα) binding profile in EOC cell lines and investigated its association with genome instability, HRR deficiency and sensitivity to chemotherapy using extensive public datasets and in vitro/in vivo experiments. Results: We found an inverse correlation between estrogen signaling and HRR activity in EOC, and the genome-wide collaboration between ERα and the co-repressor CtBP. Though the non-classical AP-1-mediated ERα signaling, their targets were highly enriched by HRR genes. We found that depleting ERα in EOC cells up-regulates HRR activity and HRR gene expression. Consequently, estrogen signaling enhances the sensitivity of ovarian cancer cells to chemotherapy agents in vitro and in vivo. Large-scale analyses further indicate that estrogen replacement and ESR1 expression are associated with chemo-sensitivity and the favorable survival of EOC patients. Conclusion: These findings characterize a novel role of ERα in mediating the molecular connection between hormone and HRR in EOC and encourage hormone replacement therapy for EOC patients.

15.
Int J Clin Oncol ; 24(11): 1479-1489, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31278532

RESUMO

BACKGROUND: We investigated the microarray data GSE42352 to identify genes that can be used as prognosis factors in osteosarcoma. METHODS: Gene Ontology (GO) biological process analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of Cytoscape ClueGo were used in verifying the function of different genes. Realtime-PCR were used to confirm the microarray results. 83 patient samples were collected and underwent Kaplan-Meier survival analysis and multivariate analysis to predict the prospect of genes using as prognosis factors. RESULTS: After analyzing the microarray data GSE42352, mitosis metaphase to anaphase-related genes CDC20, securin, cyclin A2 and cyclin B2 were found to be overexpressed in osteosarcoma cell lines. Kaplan-Meier survival analysis showed that overexpression of these genes can predict poor prognosis outcomes in osteosarcoma patients. Furthermore, any combination of the four genes seems to be more effective in predicting osteosarcoma outcomes than any of these genes alone. CONCLUSIONS: CDC20 and its downstream substracts securin, cyclin A2 and cyclin B2 are good factors that can predict prognosis outcomes in osteosarcoma. Any two combination of these four genes are more effective to be used as osteosarcoma prognosis factors.


Assuntos
Biomarcadores Tumorais/genética , Neoplasias Ósseas/genética , Proteínas Cdc20/genética , Osteossarcoma/genética , Adolescente , Adulto , Idoso , Neoplasias Ósseas/mortalidade , Linhagem Celular Tumoral , Criança , Ciclina A2/genética , Ciclina B2/genética , Bases de Dados Genéticas , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Análise de Sequência com Séries de Oligonucleotídeos , Osteossarcoma/mortalidade , Prognóstico , Securina/genética , Adulto Jovem
16.
Water Res ; 163: 114900, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31362207

RESUMO

Fenton sludge (FS) generated from Fenton process with high contents of iron and organic contaminants is regarded as a hazardous waste that requires to be properly disposed. Considering that Fe(III) compounds could stimulate dissimilatory iron reduction (DIR) and enrich iron reducing bacteria (IRB) that utilized Fe(III) as electron acceptor to oxidize organic matters, FS was introduced in anaerobic digestion (AD) reactors for treating wastewater meanwhile disposing FS. Results showed that methane production and organic matters removal significantly increased with dosing FS from 0 to 2.4 g. Also, a majority of organic matters involved in FS was mineralized, including 38.5% of PAHs removal. Humic acids (HA) with redox-activity involved in FS might affect efficiency of DIR. After extracting HA from FS, the rate and the extent of Fe(III) reduction of FS decreased by 33.2% and 13.9%, respectively. Together with analysis of the electron exchange capacity of HA, it suggested that the HA involved in FS might serve as an electron shuttle to effectively promote DIR. The increase of sludge conductivity and the enrichment of IRBs in microbial communities with dosage of FS were in agreement with the above results.


Assuntos
Substâncias Húmicas , Esgotos , Anaerobiose , Compostos Férricos , Metano , Eliminação de Resíduos Líquidos , Águas Residuárias
17.
Chemosphere ; 234: 520-527, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31229713

RESUMO

The occurrence and quantities of ARGs in extracellular and intracellular DNA (eARGs and iARGs) from sediments collected in two different types of aquaculture farms were investigated. A total of 20 ARG subtypes associated with 7 categories of commonly used antibiotics (e.g., aminoglycosides, beta-lactams, sulfonamides, tetracyclines) were identified, and some of these subtypes were not related to the antibiotics used. ARGs are mainly present in the iDNA form with the ratio of the total iARGs to eARGs being in the range of 7.9-45.5. The ratio of eARG to iARGs varies greatly with ARG subtypes, probably due to their differences in persistence as a part of eDNA. Significant correlation between int1 and ARGs was observed for both eDNA and iDNA in sediments from the aquaculture farms. Moreover, ARG pollution was more serious in bullfrog ponds than polyculture ponds due to the more frequent use of antibiotics in bullfrog rearing operations.


Assuntos
Aquicultura/métodos , DNA/genética , Resistência Microbiana a Medicamentos/genética , Tanques/química , Animais , Antibacterianos/farmacologia , Fazendas , Genes Bacterianos/efeitos dos fármacos , Sedimentos Geológicos/química , Rana catesbeiana , Tetraciclinas , beta-Lactamas
18.
J Nanosci Nanotechnol ; 19(12): 7532-7538, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31196257

RESUMO

Nanostructured Fe3O4/C composites are very attractive for high-performance magnetic targeted drug carriers. Herein, Fe3O4/C composite nanospheres with good dispersity are prepared by a simple one-step hydrothermal synthesis and subsequent heat treatment in Ar. The composite nanospheres consist of clustered primary nanoparticles, and exhibit a hierarchical architecture with a high specific surface area of 119.3 m² g-1. The Fe3O4/C composite nanospheres show a high saturation magnetization value of 101 emu g-1 and good biocompatibility. In particular, the composite nanospheres deliver a large loading content (85.8%) of epirubicin hydrochloride (EPI), resulting from their unique composition and microstructure. More importantly, the release of EPI from the EPI-loaded magnetic carrier (Fe3O4/C-EPI) may be enhanced by both a slightly acidic environment and a rotating magnetic field induced by a simple motor-driven magnet system. The above favorable properties make the hierarchical Fe3O4/C composite sample a promising candidate for magnetic targeting nanocarriers of EPI.

20.
Bioresour Technol ; 286: 121388, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31063945

RESUMO

Commercial ethanol production from straw is a series of complex processes that are energy-intensive and uneconomical. Corn straw was used as a bioethanol source to mix with waste activated sludge for improving anaerobic co-digestion (AcoD) in this study. Ethanol production from the straw after yeast fermentation was 1400-2200 mg COD/L, accounting for about 0.1% of the fermentative effluent, but methane production of the yeast-group increased by 36% compared to that of control-group with no ethanol production in advance. Volatile suspended solid removal achieved 60%, obviously higher than common anaerobic digestion (AD). Multiple lines of evidence including sludge conductance, effects of activated carbon on methanogenesis and microbial community demonstrated that ethanol from the straw fermentation stimulated direct interspecies electron transfer to be established in the digesters. The results suggested that using ethanol produced from straw was a cost-effective novel way for energy recovery from disposal of agricultural and municipal wastes.


Assuntos
Reatores Biológicos , Esgotos , Anaerobiose , Etanol , Metano
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