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J Mol Med (Berl) ; 97(11): 1575-1588, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31673738


Glioblastoma is one of the most aggressive types of brain tumor. Epidermal growth factor receptors (EGFRs) are overexpressed in glioma, and EGFR amplifications and mutations lead to rapid proliferation and invasion. EGFR-targeted therapy might be an effective treatment for glioma. Gefitinib (Ge) is an EGFR tyrosine kinase inhibitor (TKI), and Golgi phosphoprotein 3 (GOLPH3) expression is associated with worse glioma prognosis. Downregulation of GOLPH3 could promote EGFR degradation. Here, an angiopep-2 (A2)-modified cationic lipid-poly (lactic-co-glycolic acid) (PLGA) nanoparticle (A2-N) was developed that can release Ge and GOLPH3 siRNA (siGOLPH3) upon entering glioma cells and therefore acts as a combinatorial anti-tumor therapy. The in vitro and in vivo studies proved that A2-N/Ge/siGOLPH3 successfully crossed the blood-brain barrier (BBB) and targeted glioma. Released siGOLPH3 effectively silenced GOLPH3 mRNA expression and further promoted EGFR and p-EGFR degradation. Released Ge also markedly inhibited EGFR signaling. This combined EGFR-targeted action achieved remarkable anti-glioma effects and could be a safe and effective treatment for glioma. KEY MESSAGES: Angiopep-2-modified cationic lipid polymer can penetrate the BBB. Gefitinib can inhibit EGFR signaling and block the autophosphorylation of critical tyrosine residues on EGFR. GOLPH3 siRNA can be transfected into glioma and downregulate GLOPH3 expression. A2-N/Ge/siGOLPH3 can inhibit glioma growth.

J Biomed Nanotechnol ; 15(9): 1982-1993, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31387684


Radiotherapy (RT) has become one of the most effective treatments for malignant tumor. Intra-tumoral hypoxia is recognized as a chief reason that induces resistance to radiation. Moreover, the toxicities of RT to normal tissues limits the usage of high doses of radiation to eliminate cancer cells. Therefore, developing an effective radiosensitizer is critical for improving the curative effects of RT. In the present study, we developed angiopep-2 (A2) modified hypoxic lipid radiosensitizer (HLR) coated gold nanoparticles (GNPs) (referred to as A2-HRGNPs) to increase the RT sensitivity of tumors. The A2-HRGNPs are comprised of the following two functional components: (1) HLR enhances the RT sensitivity on hypoxic tumor cells; (2) alkylthiol modified GNPs (DGNPs) increase radiation effects by a dose enhancing effect in RT. Our findings suggest that the synergistic radiosensitizing effects of A2-HRGNPs can significantly enhance radiosensitization effects and thus, inhibit tumor growth in vivo.

Nanopartículas Metálicas , Neoplasias , Ouro , Humanos , Hipóxia , Lipídeos , Radiossensibilizantes
Drug Deliv ; 26(1): 34-44, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30744436


The addition of temozolomide (TMZ) to radiotherapy (RT) improves survival of patients with glioblastoma (GBM). However, TMZ + RT causes excess toxicity in patients. In this study, we prepared angiopep-2 (A2) modified lipid-poly (hypoxic radiosensitized polyprodrug) nanoparticles for TMZ delivery (A2-P(MIs)25/TMZ) to achieve synergistic effects against glioma. This A2-P(MIs)25/TMZ display highly promising advantages: (1) a hydrophobic P-(MIs)25 core where poorly water-soluble TMZ can be encapsulated; (2) nitro groups of the hydrophobic P-(MIs)25 core that are converted into hydrophilic amino groups (P(NH2s)25) under low oxygen conditions to mimic the oxygen-increased sensitization to RT; (3) a lipid monolayer at the interface of the core and the shell to modify the A2 (a specific ligand for low-density lipoprotein receptor-related protein-1 (LRP-1), which are expressed in the blood-brain barrier (BBB) and human glioma cells), thereby enhancing the drug encapsulation efficiency in glioma. These nanoparticles appear as a promising and robust nanoplatforms for TMZ and hypoxic cell radiosensitization delivery.

Antineoplásicos Alquilantes/administração & dosagem , Glioma/terapia , Nanopartículas/administração & dosagem , Peptídeos/administração & dosagem , Radiossensibilizantes/administração & dosagem , Temozolomida/administração & dosagem , Animais , Antineoplásicos Alquilantes/síntese química , Antineoplásicos Alquilantes/metabolismo , Linhagem Celular Tumoral , Terapia Combinada/métodos , Sistemas de Liberação de Medicamentos/métodos , Glioma/metabolismo , Humanos , Masculino , Camundongos , Camundongos Endogâmicos ICR , Camundongos Nus , Nanopartículas/química , Nanopartículas/metabolismo , Peptídeos/síntese química , Peptídeos/metabolismo , Copolímero de Ácido Poliláctico e Ácido Poliglicólico/administração & dosagem , Copolímero de Ácido Poliláctico e Ácido Poliglicólico/síntese química , Copolímero de Ácido Poliláctico e Ácido Poliglicólico/metabolismo , Pró-Fármacos/administração & dosagem , Pró-Fármacos/química , Pró-Fármacos/metabolismo , Radiossensibilizantes/síntese química , Radiossensibilizantes/metabolismo , Radioterapia/métodos , Temozolomida/síntese química , Temozolomida/metabolismo , Ensaios Antitumorais Modelo de Xenoenxerto/métodos