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1.
Yi Chuan ; 41(4): 285-292, 2019 Apr 20.
Artigo em Chinês | MEDLINE | ID: mdl-30992250

RESUMO

Histone methylation is a modification which occurs in the N-terminal peptide chains of the histone nucleosome. The 4th, 9th, 27th, 36th and 79th lysines in N-terminal peptide chain of histone H3 are hot spots for this modification, including mono-, di-, and tri-methylation. H3K27me3 is the tri-methylation modification on histone H3 lysine 27, which mainly functions as a transcriptional repressor regulating skeletal muscle development. Studies have shown that H3K27me3 can finely regulate skeletal muscle proliferation, including the level and duration of skeletal muscle development by specifically binding to myogenic regulatory factors (e.g., MyoD, MyoG, etc.), cell cycling regulators, and epigenetic regulators including lncRNA and miRNA. In this review, we introduce the types and mechanisms of histone methylation and de-methylation of H3K27. We also summarize how H3K27me3 functions in the proliferation and differentiation of skeletal muscle cell. This review will contribute to the comprehension of the function of H3K27me3 in regulating skeletal muscle development and provide reference for further improving our understanding of mammalian muscle.


Assuntos
Histonas/fisiologia , Desenvolvimento Muscular , Músculo Esquelético/crescimento & desenvolvimento , Animais , Proliferação de Células , Lisina/química , Mamíferos , Metilação , Células Musculares/citologia , Nucleossomos/química
2.
Yi Chuan ; 40(9): 749-757, 2018 Sep 20.
Artigo em Chinês | MEDLINE | ID: mdl-30369478

RESUMO

Non-homologous end-joining (NHEJ) is the predominant DNA double-strand break (DSB) repair pathway in mammalian cells. It inhibits the efficiency of homologous recombination (HR) by competing for DSB targets. To improve the efficiency of HR in porcine fetal fibroblasts (PFFs), several RNA interference (RNAi) systems were designed to knockdown NHEJ key molecules, such as polynucleotide kinase/phosphatase (PNKP), DNA ligase IV (LIG4) and NHEJ1. The results show that siRNA significantly knocked down LIG4, PNKP and NHEJ1 expression. Suppression of PNKP dramatically increased the efficiency of single-strand annealing (SSA), double-strand DNA (dsDNA) and single-strand DNA (ssODN) mediated homology-directed repair (HDR) by 55.7%, 37.4% and 73.1% after transfected with the SSA-GFP reporter, HDR-GFP system or ssODN-GFP system, respectively; whereas knockdown of LIG4 and NHEJ1 repair factors significantly increased dsDNA or ssODN-mediated HDR efficiency by 37.5% and 76.9%, respectively.


Assuntos
Reparo do DNA por Junção de Extremidades , Recombinação Homóloga , Interferência de RNA , Suínos/genética , Animais , DNA Ligase Dependente de ATP/genética , DNA Ligase Dependente de ATP/metabolismo , DNA de Cadeia Simples/genética , DNA de Cadeia Simples/metabolismo , Feminino , Fibroblastos/metabolismo , Técnicas de Silenciamento de Genes , Masculino , Reparo de DNA por Recombinação , Suínos/embriologia , Suínos/metabolismo
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