Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 17 de 17
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Genes Genomics ; 42(9): 997-1010, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32676852

RESUMO

BACKGROUND: Waxy maize (Zea mays L. sinensis Kulesh) is a mutant of maize (Zea mays L.) with a mutation at Waxy1 (Wx1) gene locus. The seed of waxy maize has higher viscosity compared to regular maize. By now, we know little about the expression patterns of genes that involved in the seed development of waxy maize. OBJECTIVE: By analyzing the transcriptome data during waxy maize seed development, we attempt to dig out the genes that may influence the seed development of waxy maize. METHODS: The seeds of waxy maize inbred line SWL01 from six phases after pollination were used to do RNA-seq. Bioinformatics methods were used to analyze the expression patterns of the expressed genes, to identify the genes involved in waxy maize seed development. RESULTS: A total of 24,546 genes including 1611 transcription factors (TFs) were detected during waxy maize seed development. Coexpression analysis of expressed genes revealed the dynamic processes of waxy maize seed development. Particularly, 2457 genes including 177 TFs were specially expressed in waxy maize seed, some of which mainly involved in the process of seed dormancy and maturation. In addition, 2681, 5686, 4491, 4386, 3669 and 4624 genes were identified to be differential expressed genes (DEGs) at six phases compared to regular maize B73, and 113 DEGs among them may be key genes that lead the difference of seed development between waxy and regular maizes in milk stage. CONCLUSION: In summary, we elucidated the expression patterns of expressed genes during waxy maize seed development globally. A series of genes that associated with seed development were identified in our research, which may provide an important resource for functional study of waxy maize seed development to help molecular assisted breeding.

2.
Plant Cell Environ ; 2020 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-32562291

RESUMO

The growth and development of maize are negatively affected by various abiotic stresses including drought, high salinity, extreme temperature, and strong wind. Therefore, it is important to understand the molecular mechanisms underlying abiotic stress resistance in maize. In the present work, we identified that a novel NAC transcriptional factor, ZmNST3, enhances maize lodging resistance and drought stress tolerance. ChIP-Seq and expression of target genes analysis showed that ZmNST3 could directly regulate the expression of genes related to cell wall biosynthesis which could subsequently enhance lodging resistance. Furthermore, we also demonstrated that ZmNST3 affected the expression of genes related to the synthesis of antioxidant enzyme secondary metabolites that could enhance drought resistance. More importantly, we are the first to report that ZmNST3 directly binds to the promoters of CESA5 and Dynamin-Related Proteins2A (DRP2A) and activates the expression of genes related to secondary cell wall cellulose biosynthesis. Additionally, we revealed that ZmNST3 directly binds to the promoters of GST/GlnRS and activates genes which could enhance the production of antioxidant enzymes in vivo. Overall, our work contributes to a comprehensive understanding of the regulatory network of ZmNST3 in regulating maize lodging and drought stress resistance.

3.
Int Heart J ; 61(3): 585-594, 2020 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-32418959

RESUMO

Ischemic heart disease (IHD) is one of the world's leading causes of human death. Kaempferol (Kae) was proved to have anti-inflammatory, antioxidant, and anticancer effects. Such properties suggested that it might play protective roles in IHD. In this study, we have attempted to disclose the potential regulating mechanisms of Kae in primary cardiomyocytes and H9c2 cells.Cells were first stimulated by oxygen-glucose deprivation (OGD) and then exposed to Kae. CCK-8 assay and flow cytometry were used to examine cell characteristics. Quantitative reverse-transcription polymerase chain reaction was utilized to test the expression levels of miR-15b and TLR4. Afterward, cell transfection, dual-luciferase activity assay, and western blot were used to explore the potential mechanisms.OGD treatment suppressed cell viability, whereas it enhanced cell apoptosis. Besides, OGD treatment enhanced the expression of apoptosis-associated proteins. Kae exposure, however, attenuated the effects that OGD-induced. Further experiments showed that Kae exposure promoted down-regulation of miR-15b, Bcl-2 and TLR4 were a target of miR-15b. Moreover, Kae enhanced the expression of key factors involved in PI3K/AKT and Wnt/ß-catenin pathways, whereas miR-15b mimic reversed the Kae-triggered effects.This investigation revealed that Kae diminished OGD-triggered cell damage through down-regulating miR-15b expression via activating PI3K/AKT and Wnt3a/ß-catenin pathways.


Assuntos
Quempferóis/uso terapêutico , MicroRNAs/metabolismo , Isquemia Miocárdica/tratamento farmacológico , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Receptor 4 Toll-Like/metabolismo , Animais , Linhagem Celular , Avaliação Pré-Clínica de Medicamentos , Quempferóis/farmacologia , Isquemia Miocárdica/metabolismo , Fosfatidilinositol 3-Quinases/metabolismo , Ratos Wistar , Via de Sinalização Wnt , beta Catenina/metabolismo
4.
Int J Biol Macromol ; 150: 246-252, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-32014475

RESUMO

Stigma maydis, an ingredient of pharmaceuticals and healthy foods, has a long history of usage in China and some occidental countries. Polysaccharide (SMP) is supposed to be one of the major bioactive compounds in stigma maydis, which possesses immune-enhancement, antitumor, antioxidant, anti-fatigue, diuretic, liver protection, antihyperglycaemic and hypolipidemic activities. In this review, the current advancements on extraction, purification, structural characteristics and biological activities of polysaccharides were summarized. Their biological activities were introduced on the basis of vivo experiments, and some possible mechanisms were listed. Furthermore, industrial application of SMPs were reviewed and discussed. New perspectives for the future work of stigma maydis polysaccharide were also proposed.

5.
Cell Cycle ; 19(2): 246-255, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31878833

RESUMO

Background/Aims: Myocardial ischemia (MI) is a serious threat to human health. Circular RNAs (circRNAs) play an important role in many diseases including MI. The effect and mechanism of circDENND2A in MI have not been studied.Methods: We used oxygen glucose deprivation (OGD) treatment to simulate MI in vitro. We detected circDENND2A and microRNA (miR)-34a levels by RT-qPCR. The transfection process used INTERFER and jetPRIME. Cell growth indexes including viability, apoptosis, and migration were detected by CCK8, flow cytometry, and transwell assays, respectively. In addition, the Bax, Cleaved-Caspase-3, matrix metalloproteinase (MMP)-2, MMP-9 and pathway-related protein levels were tested by Western blot.Results: OGD upregulated circDENND2A expression in H9c2 cells. Overexpression of circDENND2A enhanced cell viability and migration but declined apoptosis under OGD. Silenced circDENND 2A played the opposite effects. circDENND2A negatively regulated miR-34a. miR-34a overexpression weakened the protective effects of circDENND2A in OGD-injury. Moreover, we considered circDENND2A and miR-34a may work via ß-catenin and Ras/Raf/MEK/ERK pathways.Conclusion: circDENND2A overexpression enhanced OGD-inhibited cell viability and migration but declined OGD-promoted apoptosis by downregulating miR-34a and via ß-catenin and Ras/Raf/MEK/ERK pathways.

6.
Pharm Res ; 36(8): 118, 2019 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-31161359

RESUMO

PURPOSE: The main purposes of this manuscript are to report a surprising and interesting degradation reaction of glucagon from a specific vendor in which glucagon underwent cleavage among several peptide bonds quickly under near neutral to basic conditions, and to propose the root cause of mechanism for the degradation reaction. METHODS: The degradation reaction was monitored by HPLC and the fragment structures were confirmed by LC-MS. Possible impurities responsible for the degradation were either confirmed or excluded by a variety of techniques such as addition of chelator EDTA and transitional metal ions or separation by ultrafiltration. RESULTS: This type of degradation was rarely reported in literature, especially considering its extreme cleavage efficiency. Contamination by a thermostable high molecular impurity (such as a peptidase with molecular weight between 10 and 30 KDa) during the manufacturing process was the main reason for this interesting phenomenon. CONCLUSIONS: The degradation phenomenon described here could be used as an excellent example showing that products ordered from vendors meeting the rudimentary quality standards might contain impurities which could cause significant degradation. We suggest that a simple solution, i.e. additional tests of stability under real or accelerated conditions by manufacturers and inclusion of the "accelerated stability criteria" in the Certificate of Analysis (CoAs), especially for sensitive biological reagents prone to faster degradation, would be very helpful for avoiding losses for both vendors and users.


Assuntos
Glucagon/química , Quelantes/química , Cromatografia Líquida de Alta Pressão , Contaminação de Medicamentos , Estabilidade de Medicamentos , Ácido Edético/química , Concentração de Íons de Hidrogênio , Hidrólise , Peptídeo Hidrolases/química , Estabilidade Proteica , Espectrometria de Massas em Tandem , Elementos de Transição/química , Ultrafiltração
7.
BMC Plant Biol ; 18(1): 310, 2018 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-30497411

RESUMO

BACKGROUND: Common rust, caused by Puccinia sorghi, is an important foliar disease of maize that has been associated with up to 50% grain yield loss. Development of resistant maize germplasm is the ideal strategy to combat P. sorghi. RESULTS: Association mapping performed using a mixed linear model (MLM), integrating population structure and family relatedness identified 25 QTL (P < 3.12 × 10- 5) that were associated with resistance to common rust and distributed on chromosomes 1, 3, 5, 6, 8, and 10. We identified three QTLs associated with all three disease parameters (final disease rating, mean disease rating, and area under disease progress curve) located on chromosomes 1, 3, and 8. A total of 5 QTLs for resistance to common rust were identified in the RIL population. Nine candidate genes located on chromosomes 1, 5, 6, 8, and 10 for resistance to common rust associated loci were identified through detailed annotation. CONCLUSIONS: Using a diverse set of inbred lines genotyped with high density markers and evaluated for common rust resistance in multiple environments, it was possible to identify QTL significantly associated with resistance to common rust and several candidate genes. The results point to the need for fine mapping common rust resistance by targeting regions identified in common between this study and others using diverse germplasm.


Assuntos
Resistência à Doença/genética , Doenças das Plantas/microbiologia , Locos de Características Quantitativas/genética , Zea mays/genética , Basidiomycota , Mapeamento Cromossômico/métodos , Cromossomos de Plantas/genética , Genes de Plantas/genética , Doenças das Plantas/imunologia , Zea mays/imunologia , Zea mays/microbiologia
8.
Biomed Pharmacother ; 103: 851-857, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-29710501

RESUMO

Atherosclerosis (AS) is a chronic inflammation, which is a major cause of morbidity and mortality in the world. Accumulative evidences have demonstrated that miRNAs exert crucial roles in the development of AS. However, the effects of miR-145 and its underlying molecular mechanism remain incompletely clear. The aim of the present study is to explore the function of miR-145 in the occurrence and development of AS through investigating its role in inflammatory reactions. High-fat diet (HFD)-treated ApoE-/- mice were used as an in vivo model of atherosclerosis (AS). OxLDL-induced macrophages was employed as cell models of atherosclerosis. RT-PCR was used to evaluate the transfected efficiency of miR-145 mimic and inhibitor. RT-PCR and ELISA were performed to detect the expression of miR-145, and inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-1ß (IL-1ß), C-C motif chemokine ligand 2 (CCL-2), CCL-4 and CCL-7. Western blotting was used to evaluate the protein expression of nuclear factor κB (NF-κB) and its related proteins such as phosphorylated-signal transducer and activator of transcription 3 (p-STAT3), p-IκBα and acetylated p65 (ac-p65). Hematoxylin and eosin (H&E) staining were conducted to examine atherosclerotic lesion. Immunohistochemistry was carried out to detect the expression of α-smooth muscle Actin (α-SMA) and CD68. Luciferase reporter assay were carried out to examine the effect of miR-145 on the transcriptional activity of NF-κB. Our results showed that over-expression of miR-145 promoted the expression of IL-1ß, TNF-α, CCL-2, CCL-4 and CCL-7 through promotion of NF-κB, p-IκBα, p-STAT3 and ac-p65 expression in vivo and in vitro. Besides, down-regulation of miR-145 expression relieved the aortic sinus lesion, increased the number of VSMCs and decreased the number of macrophages. In conclusion, our study demonstrated that miR-145 accelerated the inflammatory reaction through activation of NF-κB signaling in AS.


Assuntos
Aterosclerose/metabolismo , MicroRNAs/biossíntese , NF-kappa B/metabolismo , Transdução de Sinais/fisiologia , Animais , Aterosclerose/patologia , Diferenciação Celular/fisiologia , Células Cultivadas , Humanos , Inflamação/metabolismo , Inflamação/patologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout
9.
Int J Med Mushrooms ; 19(4): 319-326, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28605320

RESUMO

Four extract fractions from the fruiting bodies of Phellinus baumii were acquired. Preliminary investigation of radical scavenging ability showed that the ethyl acetate extract exhibited the strongest antioxidant activity. To gain insight into the antioxidant mechanism, 5 antioxidant constituents were prepared from the ethyl acetate extract using high-speed countercurrent chromatography with a solvent system consisting of n-hexane, ethyl acetate, methanol, and water (0.8:3:1.5:2.5, v/v/v/v), and preparative high-performance liquid chromatography. Electrospray ionization mass spectrometry and nuclear magnetic resonance spectra were used to analyze the structures of these compounds and revealed that they were caffeic acid (compound 1), inoscavin A (compound 2), 3,4-dihydroxy benzaldehyde (compound 3), coumarin (compound 4), and naringenin (compound 5). Compound 2 showed strong antioxidant activity, and compounds 1, 3, and 5 showed moderate antioxidant activity. Thus, a more clearly and usefully scientific background is provided herein for the development of relative functional foods or biomedical products using Ph. baumii as the raw material.


Assuntos
Antioxidantes/isolamento & purificação , Basidiomycota/química , Cromatografia Líquida de Alta Pressão/métodos , Misturas Complexas/química , Distribuição Contracorrente/métodos , Antioxidantes/química , Misturas Complexas/isolamento & purificação , Espectroscopia de Ressonância Magnética , Espectrometria de Massas por Ionização por Electrospray
10.
G3 (Bethesda) ; 6(12): 3803-3815, 2016 12 07.
Artigo em Inglês | MEDLINE | ID: mdl-27742723

RESUMO

Fusarium ear rot (FER) incited by Fusarium verticillioides is a major disease of maize that reduces grain quality globally. Host resistance is the most suitable strategy for managing the disease. We report the results of genome-wide association study (GWAS) to detect alleles associated with increased resistance to FER in a set of 818 tropical maize inbred lines evaluated in three environments. Association tests performed using 43,424 single-nucleotide polymorphic (SNPs) markers identified 45 SNPs and 15 haplotypes that were significantly associated with FER resistance. Each associated SNP locus had relatively small additive effects on disease resistance and accounted for 1-4% of trait variation. These SNPs and haplotypes were located within or adjacent to 38 candidate genes, 21 of which were candidate genes associated with plant tolerance to stresses, including disease resistance. Linkage mapping in four biparental populations to validate GWAS results identified 15 quantitative trait loci (QTL) associated with F. verticillioides resistance. Integration of GWAS and QTL to the maize physical map showed eight colocated loci on chromosomes 2, 3, 4, 5, 9, and 10. QTL on chromosomes 2 and 9 are new. These results reveal that FER resistance is a complex trait that is conditioned by multiple genes with minor effects. The value of selection on identified markers for improving FER resistance is limited; rather, selection to combine small effect resistance alleles combined with genomic selection for polygenic background for both the target and general adaptation traits might be fruitful for increasing FER resistance in maize.


Assuntos
Mapeamento Cromossômico , Resistência à Doença/genética , Estudo de Associação Genômica Ampla , Interações Hospedeiro-Patógeno/genética , Doenças das Plantas/genética , Locos de Características Quantitativas , Zea mays/genética , Alelos , Cromossomos de Plantas , Fusarium , Frequência do Gene , Genética Populacional , Genômica/métodos , Genótipo , Haplótipos , Desequilíbrio de Ligação , Fenótipo , Doenças das Plantas/microbiologia , Polimorfismo de Nucleotídeo Único , Zea mays/microbiologia
12.
PLoS One ; 11(3): e0149636, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26999525

RESUMO

We aimed to identify quantitative trait loci (QTL) for secondary traits related to grain yield (GY) in two BC1F2:3 backcross populations (LPSpop and DTPpop) under well-watered (4 environments; WW) and drought stressed (6; DS) conditions to facilitate breeding efforts towards drought tolerant maize. GY reached 5.6 and 5.8 t/ha under WW in the LPSpop and the DTPpop, respectively. Under DS, grain yield was reduced by 65% (LPSpop) to 59% (DTPpop) relative to WW. GY was strongly associated with the normalized vegetative index (NDVI; r ranging from 0.61 to 0.96) across environmental conditions and with an early flowering under drought stressed conditions (r ranging from -0.18 to -0.25) indicative of the importance of early vigor and drought escape for GY. Out of the 105 detected QTL, 53 were overdominant indicative of strong heterosis. For 14 out of 18 detected vigor QTL, as well as for eight flowering time QTL the trait increasing allele was derived from CML491. Collocations of early vigor QTL with QTL for stay green (bin 2.02, WW, LPSpop; 2.07, DS, DTPpop), the number of ears per plant (bins 2.02, 2.05, WW, LPSpop; 5.02, DS, LPSpop) and GY (bin 2.07, WW, DTPpop; 5.04, WW, LPSpop), reinforce the importance of the observed correlations. LOD scores for early vigor QTL in these bins ranged from 2.2 to 11.25 explaining 4.6 (additivity: +0.28) to 19.9% (additivity: +0.49) of the observed phenotypic variance. A strong flowering QTL was detected in bin 2.06 across populations and environmental conditions explaining 26-31.3% of the observed phenotypic variation (LOD: 13-17; additivity: 0.1-0.6d). Improving drought tolerance while at the same time maintaining yield potential could be achieved by combining alleles conferring early vigor from the recurrent parent with alleles advancing flowering from the donor. Additionally bin 8.06 (DTPpop) harbored a QTL for GY under WW (additivity: 0.27 t/ha) and DS (additivity: 0.58 t/ha). R2 ranged from 0 (DTPpop, WW) to 26.54% (LPSpop, DS) for NDVI, 18.6 (LPSpop, WW) to 42.45% (LPSpop, DS) for anthesis and from 0 (DTPpop, DS) to 24.83% (LPSpop, WW) for GY. Lines out-yielding the best check by 32.5% (DTPpop, WW) to 60% (DTPpop, DS) for all population-by-irrigation treatment combination (except LPSpop, WW) identified are immediately available for the use by breeders.


Assuntos
Adaptação Fisiológica , Cruzamentos Genéticos , Secas , Locos de Características Quantitativas , Clima Tropical , Zea mays/fisiologia , Zea mays/genética
13.
Pharmazie ; 71(10): 583-587, 2016 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-29441926

RESUMO

TAK-242 (resatorvid), a novel small-molecule cyclohexene derivative, inhibits TLR4 signaling selectively. TAK-242 blocked the Toll-like receptor (TLR) 4-triggered inflammatory signaling by binding directly to a specific amino acid Cys747 in the intracellular domain of TLR4. The present study was designed to examine the effects of TAK-242 on vascular inflammatory responses in human coronary artery endothelial cells (HCAECs) challenged by lipopolysaccharide (LPS, a TLR4 ligand). The results show that TAK-242 attenuated the LPS-induced expression of interleukin (IL)-6, IL-8 and monocyte chemoattractant protein 1 both at the transcription and translation levels in HCAECs. LPS-induced endothelial cell adhesion molecules, intercellular adhesion molecular-1 and vascular cell adhesion molecule-1 expressions were also reduced by treatment with TAK-242. In addition, coincubation with TAK-242 did not effect the expression of TLR4 in LPS-activated HCAECs. Furthermore, TAK-242 efficiently suppressed LPS-induced phosphorylation of nuclear factor κB (NF-κB) and IL-1 associated kinase-1 (IRAK-1) in HCAECs. These findings show that TAK-242 can suppress endothelial cell inflammation, suggesting that TAK-242 might be suitable for development as a therapeutic agent for inflammatory cardiovascular disease.


Assuntos
Anti-Inflamatórios não Esteroides/farmacologia , Vasos Coronários/efeitos dos fármacos , Células Endoteliais/efeitos dos fármacos , Lipopolissacarídeos/antagonistas & inibidores , Sulfonamidas/farmacologia , Vasos Coronários/citologia , Citocinas/biossíntese , Citocinas/genética , Regulação da Expressão Gênica/efeitos dos fármacos , Humanos , Inflamação/induzido quimicamente , Inflamação/prevenção & controle , Quinases Associadas a Receptores de Interleucina-1/biossíntese , Quinases Associadas a Receptores de Interleucina-1/genética , NF-kappa B/efeitos dos fármacos , Moléculas de Adesão de Célula Nervosa/biossíntese , Moléculas de Adesão de Célula Nervosa/genética , RNA Mensageiro/biossíntese , Transdução de Sinais/efeitos dos fármacos , Receptor 4 Toll-Like/antagonistas & inibidores
14.
Int J Biol Macromol ; 66: 151-7, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-24513220

RESUMO

The main purpose of this manuscript is to describe a phenomenon in which vacuum degassing a reconstituted freeze-dried fusion protein etanercept formulation caused a significant amount of protein sub-visible particles (SbVP). Physical stability of etanercept was monitored by micro-flow imaging (MFI), dynamic light scattering (DLS), size-exclusion high pressure liquid chromatography (SE-HPLC) and far- and near-ultraviolet circular dichroism (far- and near-UV CD). One potential explanation of this phenomenon is that bubble collapses when the vacuum is applied, leads to substantial heat formation, and ultimately free radical formation. Subsequently, the effect of a free-radical scavenger (ascorbic acid, AA) on SbVP formation was also evaluated. Degassing of etanercept solution by applying vacuum caused substantial increase of SbVP, as detected by MFI and DLS. However, traditional techniques such as SE-HPLC could not detect any change. The addition of free-radical scavenger had minimal effect on SbVP formation, therefore the formation of free radicals was probably not the main cause for this effect.


Assuntos
Imunoglobulina G/química , Proteínas/química , Receptores do Fator de Necrose Tumoral/química , Soluções/química , Química Farmacêutica/métodos , Estabilidade de Medicamentos , Etanercepte , Depuradores de Radicais Livres/química , Liofilização/métodos , Tamanho da Partícula , Pós/química , Vácuo
15.
Glycobiology ; 24(4): 351-8, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24418930

RESUMO

The syp locus includes four genes encoding putative regulators, six genes encoding glycosyltransferases, two encoding export proteins, and six other genes encoding unidentified functional proteins associated with biofilm formation and symbiotic colonization. However, the individual functions of the respective genes remain unclear. Amino acid alignment indicates that sypQ is presumably involved in biosynthesizing poly-N-acetylglucosamine (PNAG), which is proposed to be a critical virulence factor in pathogen infection and is regarded as a target for protective immunity against a variety of Gram-negative/positive pathogens. However, no evidence showing that Vibrio parahaemolyticus also produces PNAG has been reported. Herein, the V. parahaemolyticus is confirmed to possess potential for producing PNAG for the first time. Our results indicated that gene sypQ is associated with PNAG biosynthesis and PNAG is involved in pathogen colonization. We propose that the function of pgaC in Escherichia coli could be taken over by sypQ from V. parahaemolyticus. We also tested whether PNAG can be used as a target against V. parahaemolyticus when it infects Pseudosciaena crocea. Our results showed that PNAG isolated from V. parahaemolyticus is an effective agent for decreasing V. parahaemolyticus invasion, implying that PNAG could be used to develop an effective vaccine against V. parahaemolyticus infection.


Assuntos
Acetilglucosamina/biossíntese , Acetilglucosamina/fisiologia , Genes Bacterianos , Vibrio parahaemolyticus/genética , Vibrio parahaemolyticus/patogenicidade , Acetilglucosamina/isolamento & purificação , Animais , Vacinas Bacterianas/imunologia , Genes Bacterianos/genética , Genes Bacterianos/fisiologia , Perciformes/microbiologia , Vibrioses/imunologia , Vibrioses/metabolismo , Vibrioses/prevenção & controle , Vibrio parahaemolyticus/metabolismo
16.
PLoS One ; 8(6): e66606, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23818949

RESUMO

Waxy maize (Zea mays L. var. certaina Kulesh), with many excellent characters in terms of starch composition and economic value, has grown in China for a long history and its production has increased dramatically in recent decades. However, the evolution and origin of waxy maize still remains unclear. We studied the genetic diversity of Chinese waxy maize including typical landraces and inbred lines by SSR analysis and the results showed a wide genetic diversity in the Chinese waxy maize germplasm. We analyzed the origin and evolution of waxy maize by sequencing 108 samples, and downloading 52 sequences from GenBank for the waxy locus in a number of accessions from genus Zea. A sharp reduction of nucleotide diversity and significant neutrality tests (Tajima's D and Fu and Li's F*) were observed at the waxy locus in Chinese waxy maize but not in nonglutinous maize. Phylogenetic analysis indicated that Chinese waxy maize originated from the cultivated flint maize and most of the modern waxy maize inbred lines showed a distinct independent origin and evolution process compared with the germplasm from Southwest China. The results indicated that an agronomic trait can be quickly improved to meet production demand by selection.


Assuntos
Evolução Molecular , Variação Genética , Proteínas de Plantas/genética , Sintase do Amido/genética , Zea mays/genética , Amilopectina/metabolismo , Sequência de Bases , China , Genoma de Planta/genética , Repetições de Microssatélites/genética , Dados de Sequência Molecular , Filogenia , Proteínas de Plantas/metabolismo , Análise de Sequência de DNA , Especificidade da Espécie , Sintase do Amido/metabolismo , Zea mays/classificação , Zea mays/metabolismo
17.
Acta Pharmacol Sin ; 32(11): 1345-50, 2011 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-21963897

RESUMO

AIM: To investigate the possible effects of telmisartan and losartan on cardiac function in adriamycin (ADR)-induced heart failure in rats, and to explore the changes in plasma level of angiotensin-(1-7)[Ang-(1-7)] and myocardial expression of angiotensin II type 1/2 receptors (AT(1)R / AT(2)R) and Mas receptor caused by the two drugs. METHODS: Male Sprague-Dawley rats were randomly divided into 4 groups: the control group, ADR-treated heart failure group (ADR-HF), telmisartan plus ADR-treated group (Tel+ADR) and losartan plus ADR-treated group (Los+ADR). ADR was administrated (2.5 mg/kg, ip, 6 times in 2 weeks). The rats in the Tel+ADR and Los+ADR groups were treated orally with telmisartan (10 mg/kg daily po) and losartan (30 mg/kg daily), respectively, for 6 weeks. The plasma level of Ang-(1-7) was determined using ELISA. The mRNA and protein expression of myocardial Mas receptor, AT(1)R and AT(2)R were measured using RT-PCR and Western blotting, respectively. RESULTS: ADR significantly reduced the plasma level of Ang-(1-7) and the expression of myocardial Mas receptor and myocardial AT(2)R, while significantly increased the expression of myocardial AT(1)R. Treatment with telmisartan and losartan effectively increased the plasma level of Ang-(1-7) and suppressed myocardial AT(1)R expression, but did not influence the expression of Mas receptor and AT(2)R. CONCLUSION: The protective effects of telmisartan and losartan in ADR-induced heart failure may be partially due to regulation of circulating Ang-(1-7) and myocardial AT(1)R expression.


Assuntos
Bloqueadores do Receptor Tipo 1 de Angiotensina II/uso terapêutico , Angiotensina I/sangue , Antibióticos Antineoplásicos/efeitos adversos , Benzimidazóis/uso terapêutico , Benzoatos/uso terapêutico , Insuficiência Cardíaca/tratamento farmacológico , Losartan/uso terapêutico , Fragmentos de Peptídeos/sangue , Receptor Tipo 1 de Angiotensina/genética , Angiotensina I/metabolismo , Bloqueadores do Receptor Tipo 1 de Angiotensina II/farmacologia , Animais , Benzimidazóis/farmacologia , Benzoatos/farmacologia , Doxorrubicina/efeitos adversos , Regulação da Expressão Gênica/efeitos dos fármacos , Coração/efeitos dos fármacos , Insuficiência Cardíaca/induzido quimicamente , Losartan/farmacologia , Masculino , Miocárdio/metabolismo , Fragmentos de Peptídeos/metabolismo , Ratos , Ratos Sprague-Dawley , Receptor Tipo 1 de Angiotensina/metabolismo , Telmisartan
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA