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1.
Plant Dis ; : PDIS05201052A, 2020 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-33200956

RESUMO

Sphingomonas carotinifaciens strain L9-754T (DSM 27347) is a gram-negative, chemoheterotrophic, and rod-shaped endophyte isolated from the stem tissues of Jatropha curcas L. This strain has putative in vitro antagonistic ability against the plant pathogenic fungus Magnaporthe grisea. A draft genome of L9-754T was obtained using the PacBio SMRT cell platform. By analyzing the genome of strain L9-754T, a gene cluster (GQR91_18700 - GQR91_18715) related to an antioxidant enzyme was identified in the obtained draft genome. The information obtained from the draft genome is expected to reveal the putative properties helpful in biocontrol applications.

2.
Nat Commun ; 11(1): 4778, 2020 09 22.
Artigo em Inglês | MEDLINE | ID: mdl-32963241

RESUMO

Obtaining genetic variation information from indica rice hybrid parents and identification of loci associated with heterosis are important for hybrid rice breeding. Here, we resequence 1,143 indica accessions mostly selected from the parents of superior hybrid rice cultivars of China, identify genetic variations, and perform kinship analysis. We find different hybrid rice crossing patterns between 3- and 2-line superior hybrid lines. By calculating frequencies of parental variation differences (FPVDs), a more direct approach for studying rice heterosis, we identify loci that are linked to heterosis, which include 98 in superior 3-line hybrids and 36 in superior 2-line hybrids. As a proof of concept, we find two accessions harboring a deletion in OsNramp5, a previously reported gene functioning in cadmium absorption, which can be used to mitigate rice grain cadmium levels through hybrid breeding. Resource of indica rice genetic variation reported in this study will be valuable to geneticists and breeders.


Assuntos
Variação Genética , Vigor Híbrido/genética , Oryza/genética , Cruzamento , Proteínas de Transporte de Cátions/genética , China , Cruzamentos Genéticos , Deleção de Genes , Genes de Plantas , Hibridização Genética , Oryza/classificação , Filogenia , Proteínas de Plantas/genética , Polimorfismo de Nucleotídeo Único
3.
Front Plant Sci ; 9: 560, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29755498

RESUMO

Premature leaf senescence (PLS), which has a significant impact on yield, is caused by various underlying mechanisms. Glycosyltransferases, which function in glycosyl transfer from activated nucleotides to aglycones, are involved in diverse biological processes, but their roles in rice leaf senescence remain elusive. Here, we isolated and characterized a leaf senescence-related gene from the Premature Leaf Senescent mutant (pls2). The mutant phenotype began with leaf yellowing at tillering and resulted in PLS during the reproductive stage. Leaf senescence was associated with an increase in hydrogen peroxide (H2O2) content accompanied with pronounced decreases in net photosynthetic rate, stomatal conductance, and transpiration rate. Map-based cloning revealed that a mutation in LOC_Os03g15840 (PLS2), a putative glycosyltransferase- encoding gene, was responsible for the defective phenotype. PLS2 expression was detected in all tissues surveyed, but predominantly in leaf mesophyll cells. Subcellular localization of the PLS2 was in the endoplasmic reticulum. The pls2 mutant accumulated higher levels of sucrose together with decreased expression of sucrose metabolizing genes compared with wild type. These data suggested that the PLS2 allele is essential for normal leaf senescence and its mutation resulted in PLS.

4.
J Glob Antimicrob Resist ; 14: 1-3, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-29775788

RESUMO

OBJECTIVES: Achromobacter spanius is a newly described, non-fermenting, Gram-negative, coccoid pathogen isolated from human blood. Whole-genome sequencing of the A. spanius type strain was performed to investigate the mechanism of pathogenesis of this strain at a genomic level. METHODS: The complete genome of A. spanius type strain DSM 23806T was sequenced using single-molecule real-time (SMRT) DNA sequencing. RESULTS: The complete genome of DSM 23806T consists of one circular DNA chromosome of 6425783bp with a G+C content of 64.26%. The entire genome contains 5804 predicted coding sequences (CDS) and 55 tRNAs. Genomic island (GI) analysis showed that this strain encodes several important pathogenesis- and resistance-related genes. CONCLUSIONS: These results strongly suggest that GIs provide some fitness advantages in A. spanius type strain DSM 23806T. This report provides an extensive understanding of A. spanius at a genomic level as well as an understanding of the evolution of A. spanius.


Assuntos
Achromobacter/genética , Achromobacter/patogenicidade , Genoma Bacteriano , Achromobacter/isolamento & purificação , China , DNA Bacteriano/genética , Genômica , Infecções por Bactérias Gram-Negativas/sangue , Humanos , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Sequenciamento Completo do Genoma
5.
Pathog Dis ; 75(4)2017 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-28369432

RESUMO

Achromobacter insolitus is a newly described, Gram-negative, small (1-2 mm long) and coccoid pathogen that has been isolated from leg wound. Due to its potential threat to human beings, the type strain of this species LMG 6003T was completely sequenced in this study. The genomic analysis revealed that the genome of LMG 6003T consists of one circular DNA chromosome of 6 492 697 bp with a GC content of 65.10%. The entire genome contains 6208 predicted coding genes, 61 tRNAs and 13 rRNA genes. Comparative genome analysis between LMG 6003T and another selected 10 sequenced Achromobacter revealed that 725 genes only exist in LMG 6003T. In particular, these genes include several important pathogenic-related genes such as Type IV prepilin peptidase (TadV/CpaA), Type VI secretion lipoprotein (VasD) and type 1 fimbriae major subunit (FimA). Genomic island (GI) analysis also suggests the existence of GIs in LMG 6003T. All these results strongly suggest the unique genomic features in LMG 6003T against other Achromobacter species. This report will provide us with an extended understanding of A. insolitus at the genomic level and would be helpful for understanding the evolution of the Achromobacter genus.


Assuntos
Achromobacter/genética , Mapeamento Cromossômico , DNA Bacteriano/genética , Regulação Bacteriana da Expressão Gênica , Genoma Bacteriano , Achromobacter/classificação , Achromobacter/isolamento & purificação , Composição de Bases , DNA Bacteriano/metabolismo , DNA Circular/genética , DNA Circular/metabolismo , Endopeptidases/genética , Endopeptidases/metabolismo , Proteínas de Fímbrias/genética , Proteínas de Fímbrias/metabolismo , Ontologia Genética , Ilhas Genômicas , Humanos , Perna (Membro)/microbiologia , Anotação de Sequência Molecular , RNA Ribossômico 16S/genética , RNA Ribossômico 16S/metabolismo , RNA de Transferência/genética , RNA de Transferência/metabolismo , Sistemas de Secreção Tipo VI/genética , Sistemas de Secreção Tipo VI/metabolismo , Sequenciamento Completo do Genoma
6.
Yi Chuan ; 31(8): 849-54, 2009 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-19689947

RESUMO

Hybrid rice breeding is the combining ability breeding. Screening hybrid rice combinations with high special combining ability (SCA) is able to breed strong superiority combinations with practical values. In this study, the genetic distances (GD) of nine three-line hybrid rice (5 CMS lines and 4 restorer lines) were examined using SSR markers. Based on yield performances of 20 hybrid crosses (5 x 4 NCII), the relationships between SCA, heterosis and GD were studied. The correlations of yield SCA with the control heterosis (r1=0.5609) and the average heterosis (r2=0.541) were significant, but not significant with GD (r=0.2143). Thus, the heterosis can be reflected by SCA; the hybrid parents selected in this study can be used to develop strong superiority combinations; but the SCA cannot be reflected by GD, which needs further study.


Assuntos
Quimera/genética , Vigor Híbrido , Oryza/genética , Cruzamento , Quimera/classificação , Quimera/crescimento & desenvolvimento , Oryza/classificação , Oryza/crescimento & desenvolvimento , Fenótipo , Filogenia
7.
J Plant Physiol ; 165(3): 324-30, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-17306416

RESUMO

A single dominant gene Gc controls the trait of high chlorophyll (Chl) content in rice (cultivar (cv.) Zhenshan 97B). The contents of Chl b and total Chl increased 100% and 25%, respectively, when Gc was introduced. In addition, photosynthetic rate, biomass and grain yield also increased by 20%, 17% and 16%, respectively. Three simple sequence repeats (SSR) markers (rm462, rm6340 and rm6464) that are linked to Gc were identified by amplification of DNA samples from near-isogenic lines using two hundred pairs of primers. The genetic distances on the short arm of rice chromosome 1 between Gc and rm6464, rm6340 and rm462 were 0, 0.588 and 1.18 cM, respectively.


Assuntos
Clorofila/metabolismo , Genes de Plantas , Oryza/metabolismo , Biomassa , Eletroforese em Gel de Poliacrilamida , Proteínas de Membrana/metabolismo , Oryza/genética , Oryza/fisiologia , Fotossíntese , Tilacoides/metabolismo
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