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1.
Cancer Biol Ther ; : 1-11, 2021 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-33612070

RESUMO

Stearoyl-CoA-desaturase 1 (SCD1) deficiency mediates apoptosis in colorectal cancer cells by promoting ceramide de novo synthesis. The mechanisms underlying the cross-talk between SCD1 and ceramide synthesis have not been explored. We treated colorectal cancer cells with an SCD1 inhibitor and examined the effects on gene expression, cell growth, and cellular lipid contents. The main effect of SCD1 inhibition on the fatty acid contents of colorectal cancer cells was a decrease in monounsaturated fatty acids (MUFAs). RNA sequencing (RNA-seq) showed that the most intense alteration of gene expression after SCD1 inhibition occurred in the NF-κB signaling pathway. Further experiments revealed that SCD1 inhibition resulted in increased levels of phosphorylated NF-κB p65 and increased nuclear translocation of NF-κB p65. Treatment with an NF-κB inhibitor eliminated several effects of SCD1 inhibition, mainly including overexpression of serine palmitoyltransferase1 (SPT1), elevation of dihydroceramide contents, and suppression of cell growth. Furthermore, treatment with supplemental oleate counteracted the SCD1-induced NF-κB activation and downstream effects. In summary, our data demonstrate that the NF-κB pathway plays a role in SCD1 deficiency-induced ceramide de novo synthesis in colorectal cancer cells, and that reduced MUFA levels contribute to the course.

2.
Ecotoxicology ; 2021 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-33587250

RESUMO

Copper (Cu) is one of the environmental contaminations which can pose significant risks for organisms. The current study explores the effects of Cu exposure on the growth, intestinal histology and microbial ecology in Bufo gargarizans. The results revealed that 0.5-1 µM Cu exposure induced growth retardation (including reduction of total body length and wet weight) and intestinal histological injury (including disordered enterocyte, changes in the villi and vacuoles) of tadpoles. Also, high-throughput sequencing analysis showed that Cu exposure caused changes in richness, diversity and structure of intestinal microbiota. Moreover, the composition of intestinal microbiota was altered in tadpoles exposed to different concentrations of Cu. At the phylum level, we observed the abundance of proteobacteria was increased, while the abundance of fusobacteria was decreased in the intestinal microbiota of tadpoles exposed to 1 µM Cu. At the genus level, a reduced abundance of kluyvera and aeromonas was observed in the intestinal microbiota of tadpoles under the exposure of 0-0.5 µM Cu. Finally, functional predictions revealed that tadpoles exposed to copper may be at a higher risk of developing metabolic disorders or diseases. Above all, our results will develop a comprehensive view of the Cu exposure in amphibians and will yield a new consideration for sublethal effects of Cu on aquatic organisms.

3.
Biomed Environ Sci ; 34(1): 9-18, 2021 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-33531103

RESUMO

Objective: The relationship between serum uric acid (SUA) levels and glycemic indices, including plasma glucose (FPG), 2-hour postload glucose (2h-PG), and glycated hemoglobin (HbA1c), remains inconclusive. We aimed to explore the associations between glycemic indices and SUA levels in the general Chinese population. Methods: The current study was a cross-sectional analysis using the first follow-up survey data from The China Cardiometabolic Disease and Cancer Cohort Study. A total of 105,922 community-dwelling adults aged ≥ 40 years underwent the oral glucose tolerance test and uric acid assessment. The nonlinear relationships between glycemic indices and SUA levels were explored using generalized additive models. Results: A total of 30,941 men and 62,361 women were eligible for the current analysis. Generalized additive models verified the inverted U-shaped association between glycemic indices and SUA levels, but with different inflection points in men and women. The thresholds for FPG, 2h-PG, and HbA1c for men and women were 6.5/8.0 mmol/L, 11.0/14.0 mmol/L, and 6.1/6.5, respectively (SUA levels increased with increasing glycemic indices before the inflection points and then eventually decreased with further increases in the glycemic indices). Conclusion: An inverted U-shaped association was observed between major glycemic indices and uric acid levels in both sexes, while the inflection points were reached earlier in men than in women.


Assuntos
Índice Glicêmico , Ácido Úrico/sangue , Idoso , Grupo com Ancestrais do Continente Asiático , Glicemia/análise , China/epidemiologia , Estudos de Coortes , Diabetes Mellitus/sangue , Feminino , Teste de Tolerância a Glucose , Hemoglobina A Glicada/análise , Humanos , Masculino , Pessoa de Meia-Idade
4.
Ann Palliat Med ; 2021 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-33548995

RESUMO

BACKGROUND: To explore the prognostic role of ovarian endometriosis in symptomatic adenomyosis patients underwent uterine artery embolization (UAE). METHODS: This was a retrospective, single-center study. A total of 76 patients with adenomyosis who underwent UAE in The First Affiliated Hospital of Sun Yat-sen University between May 2009 and July 2016 were enrolled in this study. These patients were divided into two groups based on whether complicated with ovarian endometriosis. After UAE, the patients were followed up for 12 months. The improvements of dysmenorrhea and menorrhagia were evaluated according to the symptom relief criteria. The improvement rates in both groups were analyzed and compared. RESULTS: Among the 76 patients with adenomyosis, 17 (22.3%) were diagnosed with OE and 59 (77.6%) were non-OE. In the OE group, all patients (17/17, 100%) had dysmenorrhea and 11 (11/17, 64.7%) had menorrhagia. In non-OE group, 57 patients (57/59, 96.6%) had dysmenorrhea and 50 (50/59, 84.7%) had menorrhagia. The improvement rates of dysmenorrhea in the two groups were 47.1% (OE group) and 86.0% (non-OE group), respectively (P<0.05). The improvement rates of menorrhagia in the two groups were 63.6% (OE group) and 84.0% (non-OE group), respectively (P=0.263). CONCLUSIONS: Patients without OE showed a lower incidence of dysmenorrhea and may have an advantage in the improvement of dysmenorrhea compared with those with OE when they underwent UAE. However, no significant difference was observed in the improvement of menorrhagia.

6.
Biomed Res Int ; 2021: 8835275, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33506038

RESUMO

Biocontrol by inoculation with beneficial microbes is a proven strategy for reducing the negative effect of soil-borne pathogens. We evaluated the effects of microbial inoculants BIO-1 and BIO-2 in reducing soil-borne wheat diseases and in influencing wheat rhizosphere microbial community composition in a plot test. The experimental design consisted of three treatments: (1) Fusarium graminearum F0609 (CK), (2) F. graminearum + BIO-1 (T1), and (3) F. graminearum F0609 + BIO-2 (T2). The results of the wheat disease investigation showed that the relative efficacies of BIO-1 and BIO-2 were up to 82.5% and 83.9%, respectively. Illumina MiSeq sequencing revealed that bacterial abundance and diversity were significantly higher (P < 0.05) in the treatment groups (T1 and T2) than in the control, with significantly decreased fungal diversity in the T2 group. Principal coordinates and hierarchical clustering analyses revealed that the bacterial and fungal communities were distinctly separated between the treatment and control groups. Bacterial community composition analysis demonstrated that beneficial microbes, such as Sphingomonas, Bacillus, Nocardioides, Rhizobium, Streptomyces, Pseudomonas, and Microbacterium, were more abundant in the treatment groups than in the control group. Fungal community composition analysis revealed that the relative abundance of the phytopathogenic fungi Fusarium and Gibberella decreased and that the well-known beneficial fungi Chaetomium, Penicillium, and Humicola were more abundant in the treatment groups than in the control group. Overall, these results confirm that beneficial microbes accumulate more easily in the wheat rhizosphere following application of BIO-1 and BIO-2 and that the relative abundance of phytopathogenic fungi decreased compared with that in the control group.

7.
Artigo em Inglês | MEDLINE | ID: mdl-33491567

RESUMO

Primary hyperoxaluria type 1 (PH1) is a severe inherited disorder caused by a genetic defect in alanine-glyoxylate aminotransferase (AGXT) that results in recurrent urolithiasis and renal failure. Animal models that precisely reflect the human PH1 phenotypes are lacking. We aimed to develop a novel PH1 rat model and study the mechanisms involved in PH1 deterioration. One-cell stage Sprague-Dawley embryos were injected with the CRISPR/Cas9 system to introduce a Q84X mutation in Agxt. Liver tissues were harvested for determining Agxt expression. Urine oxalate, crystals, and electrolyte levels in AgxtQ84X and wild-type (WT) littermates were evaluated. Kidney tissues were used for Pizzolato staining and kidney injury evaluation. Data showed that Agxt mRNA and protein were absent in AgxtQ84X rats. At 4 and 24 weeks, the AgxtQ84X rats displayed 2.3- and 3-fold higher urinary oxalate levels, respectively, compared with WT littermates. As a result, calcium oxalate (CaOx) crystals in urine were revealed in all AgxtQ84X rats, but none of the WT rats. We also observed bladder stones in 36.4% of the AgxtQ84X rats, of which 44.4% had renal CaOx deposition. Moreover, the elevated serum urea and creatinine levels indicated the impaired renal function in AgxtQ84X rats. Further investigation revealed significantly increased expression of inflammation-, necroptosis-, and fibrosis-related genes in the kidneys of AgxtQ84X rats with spontaneous CaOx deposition, indicating that these pathways are involved in PH1 deterioration. Collectively, these results suggest that this rat model has broad applicability in mechanistic studies and innovative therapeutics development for PH1 and other kidney stone diseases.

8.
Chemosphere ; 271: 129511, 2021 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-33445016

RESUMO

The gastrointestinal tract is the largest immune organ in the body and meanwhile, accommodates a large number of microorganisms. Heavy metals could disturb the intestinal homeostasis and change the gut microbial composition. However, the information regarding the links between dysbiosis of gut microbiota and imbalance of host intestinal homeostasis induced by the mixture of heavy metals is insufficient. The present study investigates the effects of Cd/Pb, both single and combination exposure, on the growth performance, intestinal histology, digestive enzymes activity, oxidative stress and immune parameters, and intestinal microbiota in Bufo gargarizans tadpoles. Our results revealed that co-exposure of Cd-Pb induced more severe impacts not only on the host, but the intestinal microbiota. On the one hand, co-exposure of Cd-Pb significantly induced growth retardation, intestinal histological injury, decreased activities of digestive enzymes. On the other hand, Cd and Pb exposure, especially in mixed form, changed the diversity and richness, structure of microbiota. Also, the intestinal microbial composition was altered by Cd/Pb exposure (alone and combination) both at the different levels. Proteobacteria, act as front-line responder, was significantly increased in tadpoles under the exposure of metals. Finally, the functional prediction revealed that the disorders of metabolism and immune responses of intestinal microbiota was increased in tadpoles exposed to Cd/Pb (especially the mixture of Cd and Pb). Our research complements the understanding of links between changes in host fitness loss and intestinal microbiota and will add a new dimension of knowledge to the ecological risks of mixed heavy metals in amphibian.

9.
Sensors (Basel) ; 21(1)2021 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-33466335

RESUMO

The network security situation of campus networks on CERNET (China Education and Research Network) has received great concern. However, most network managers have no complete picture of the network security because of its special management and the rapid growth of network assets. In this investigation, the security of campus networks belonging to seven universities in Wuhan was investigated. A tool called "WebHunt" was designed for campus networks, and with its help, the network security risks were found. Differently from existing tools for network probing, WebHunt can adopt the network scale and special rules of the campus network. According to the characteristics of campus websites, a series of functions were integrated into WebHunt, including reverse resolution of domain names, active network detection and fingerprint identification for software assets. Besides, WebHunt builds its vulnerability intelligence database with a knowledge graph structure and locates the vulnerabilities through matching knowledge graph information. Security assessments of seven universities presents WebHunt's applicability for campus networks. Besides, it also shows that many security risks are concealed in campus networks, such as non-compliance IP addresses and domain names, system vulnerabilities and so on. The security reports containing risks have been sent to the relevant universities, and positive feedback was received.

10.
Artigo em Inglês | MEDLINE | ID: mdl-33433945

RESUMO

The fabrication of functional assemblies with defined structures through controllable molecular packing in the physiological conditions is of great challenge. Here, we modularly design peptide-cyanine conjugates that intracellularly self-assembly into 1D columnar superstructures with controlled cyanine aggregation, exhibiting distinct imaging or photothermal properties. Peptide backbone is cleaved by caspase-3/7 after entering cells. Then self-assembled residue with double cyanine substitution (Pr-2Cy) arranges into P helical column, in which H-aggregated cyanine dyes show 3.4-fold photothermal conversion efficiency compared to free ones. While self-assembled residue with single cyanine substitution (Pr-1Cy) forms loose column, in which cyanine dyes with undefined structure keeps fluorescence quantum yield up to 9.5 % (emission at 819 nm in H2O). This work provides a simple way to modify in vivo self-assembled peptide with functional molecules for achieving desired bio-applications.

11.
Nat Prod Bioprospect ; 2021 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-33389669

RESUMO

Four new 3,4-secocycloartane triterpenoids, pseudolactones A-D (1-4), were isolated from the ethanol extract of the cones of Pseudol arixamabilis. Their structures were established by extensive 1D- and 2D-NMR experiments. The cones of P. arixamabilis are enriched in the ring-expanded or cleaved cycloartane triterpenoids. This work provides new insight into cycloartane triterpenoids from the cones of P. arixamabilis.

12.
J Hazard Mater ; 402: 123493, 2021 01 15.
Artigo em Inglês | MEDLINE | ID: mdl-32707467

RESUMO

An alternative risk assessment strategy for mercury (Hg)-contaminated sites is proposed with bioaccessible fractions and soil Hg vapor (SHgV) concentrations. The new strategy avoids the conservatism of assessment rely on soil total Hg (THg) content and inaccuracy caused by predicted SHgV concentration. The exposure risk to Hg-contaminated soil associated with historical mining activities in Guizhou, China, was evaluated using the proposed strategy. The experimental results revealed that the average bioaccessibility in gastric, intestinal and lung phases was 10.39 % (2.09 % ∼ 35.28 %), 1.28 % (0.23 % ∼ 4.3 %), and 11.27 % (5.04 % ∼ 20.71 %), respectively. Via the proposed strategy, the Hg risk for the oral ingestion pathway, represented as the hazard quotient (HQ), decreased from 1.57 to an acceptable level of 0.19 (<1). The risk of SHgV inhalation sharply decreased from 1168 to 0.35 while the soil PM10 inhalation pathway did not exhibit significant variations. The dominant exposure pathways turned to oral intake and inhalation of SHgV by the strategy. The results indicated that the proposed assessment strategy can greatly improve the understanding of the exposure risk level at Hg-contaminated sites and provide a reasonable decision basis for decision makers.

13.
Environ Int ; 147: 106309, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33338681

RESUMO

BACKGROUND: Fine particulate matter (PM2.5) is suspected to increase the risk of colorectal cancer, but the mechanism remains unknown. We aimed to investigate the association between PM2.5 exposure, genetic variants and colorectal cancer risk in the Prostate, Lung, Colon and Ovarian (PLCO) Cancer Screening trial. METHODS: We included a prospective cohort of 139,534 cancer-free individuals from 10 United States research centers with over ten years of follow-up. We used a Cox regression model to assess the association between PM2.5 exposure and colorectal cancer incidence by calculating the hazard ratio (HR) and 95% confidence interval (CI) with adjustment for potential confounders. The polygenic risk score (PRS) and genome-wide interaction analysis (GWIA) were used to evaluate the multiplicative interaction between PM2.5 exposure and genetic variants in regard to colorectal cancer risk. RESULTS: After a median of 10.43 years of follow-up, 1,666 participants had been diagnosed with colorectal cancer. PM2.5 exposure was significantly associated with an increased risk of colorectal cancer (HR = 1.27; 95% CI = 1.17-1.37 per 5 µg/m3 increase). Five independent susceptibility loci reached statistical significance at P < 1.22 × 10-8 in the interaction analysis. Furthermore, a joint interaction was observed between PM2.5 exposure and the PRS based on these five loci with colorectal cancer risk (P = 3.11 × 10-29). The Gene Ontology analysis showed that the vascular endothelial growth factor (VEGF) receptor signaling pathway was involved in the biological process of colorectal cancer. CONCLUSIONS: Our large-scale analysis has shown for the first time that long-term PM2.5 exposure potential increases colorectal cancer risk, which might be modified by genetic variants.

14.
Nucleic Acids Res ; 49(1): 90-97, 2021 01 11.
Artigo em Inglês | MEDLINE | ID: mdl-33330918

RESUMO

PIWI-interacting RNAs (piRNAs) are an emerging class of non-coding RNAs involved in tumorigenesis. Expression quantitative trait locus (eQTL) analysis has been demonstrated to help reveal the genetic mechanism of single nucleotide polymorphisms (SNPs) in cancer etiology. However, there are no databases that have been constructed to provide an eQTL analysis between SNPs and piRNA expression. In this study, we collected genotyping and piRNA expression data for 10 997 samples across 33 cancer types from The Cancer Genome Atlas (TCGA). Using linear regression cis-eQTL analysis with adjustment of appropriate covariates, we identified millions of SNP-piRNA pairs in tumor (76 924 831) and normal (24 431 061) tissues. Further, we performed differential expression and survival analyses, and linked the eQTLs to genome-wide association study (GWAS) data to comprehensively decipher the functional roles of identified cis-piRNA eQTLs. Finally, we developed a user-friendly database, piRNA-eQTL (http://njmu-edu.cn:3838/piRNA-eQTL/), to help users query, browse and download corresponding eQTL results. In summary, piRNA-eQTL could serve as an important resource to assist the research community in understanding the roles of genetic variants and piRNAs in the development of cancers.


Assuntos
Regulação Neoplásica da Expressão Gênica/genética , Polimorfismo de Nucleotídeo Único/genética , Locos de Características Quantitativas/genética , RNA Neoplásico/genética , RNA Interferente Pequeno/biossíntese , Bases de Dados Genéticas , Estudo de Associação Genômica Ampla , Genótipo , Humanos , Estimativa de Kaplan-Meier , Neoplasias/genética , Neoplasias/mortalidade , RNA Interferente Pequeno/genética , Interface Usuário-Computador
15.
J Ethnopharmacol ; 265: 113301, 2021 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-32860891

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Xuebijing (XBJ) injection is a Chinese medicine containing extracts from Carthamus tinctorius L. (Carthami Flos, hong hua, Asteraceae), Paeonia lactiflora Pall. (Paeoniae radix rubra, chi shao, Ranunculaceae), Ligusticum chuanxiong Hort. (Chuanxiong Rhizoma, chuan xiong, Umbelliferae), Salvia miltiorrhiza Bge. (Salviae miltiorrhizae Radix Et Rhizoma, dan shen, Labiatae) and Angelica sinensis (Oliv.) Diels (Angelicae sinensis Radix, dang gui, Umbelliferae). It has been approved for the treatment of sepsis in China since 2004 and has been widely used as an add-on treatment for sepsis or septic shock with few side effects. AIM OF THE STUDY: The aim of the present review was to analyse up-to-date information related to the treatment of sepsis with XBJ, including the bioactive constituents, clinical studies and potential mechanisms, and to discuss possible scientific gaps, to provide a reliable reference for future studies. MATERIALS AND METHODS: Scientific resources concentrating on treating sepsis with XBJ were searched through PubMed, the Chinese National Knowledge Infrastructure (CNKI) and WanFang databases from inception to November 2018. Dissertations were also searched, and eligible dissertations were selected. Studies related to the identification of constituents, bioactive components and their targets of action or pathways, clinical trials, and animal or cellular experiments that explored pharmacological mechanisms were manually selected. The quality of reporting and methodology of the included pharmacological experiments were assessed using the Animal Research: Reporting of In Vivo Experiments (ARRIVE) guidelines and the Systematic Review Centre for Laboratory Animal Experimentation (SYRCLE)'s risk of bias tool. RESULTS: A total of 108 relative studies were eventually included, containing 12 bioactivity research studies, 10 systematic reviews on clinical trials and 86 animal or cellular experiments. We noted that as identification methods progressed, further constituents could be detected in XBJ. XBJ was also found to have "multi-ingredient, multi-target and multi-pathway" effects. The systematic review revealed that XBJ could improve the 28-day mortality and other indexes, such as the APACHE II score, body temperature, and white blood cell (WBC) count, to some extent. A major organ protection effect was demonstrated in septic rats. Pharmacological investigations suggested that XBJ acts in both the early and late stages of sepsis by anti-inflammatory, anti-coagulation, immune regulation, vascular endothelial protection, anti-oxidative stress and other mechanisms. However, most of the included studies were poorly reported, and the risk of bias was unclear. CONCLUSIONS: With respect to the multiple therapeutic mechanisms contributing to both the early and late stages of sepsis, the multiple effective constituents detected and randomized controlled trials (RCTs) performed to prove its efficacy, XBJ is a promising therapy for the treatment of sepsis. However, although XBJ has shown some efficacy for the treatment of sepsis, there are currently some scientific gaps. More studies concerning the pharmacokinetics, interactions with antibiotics, real-world efficacy and safety, pharmacological mechanisms of the bioactive components and large-scale clinical trials should be conducted in the future.

16.
Neuropsychiatr Dis Treat ; 16: 2817-2825, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33262596

RESUMO

Purpose: Nitrous oxide (N2O) abuse has become an increasingly severe problem in China. The aim of the study was to summarize the features of N2O-induced neurology and enhance the awareness of this disease among physicians. Patients and Methods: We retrospectively reviewed the clinical, imaging, electrophysiological characteristics and the prognosis of patients with N2O neurotoxicity in our hospital from January 2016 to August 2019. Results: Twenty-one patients (average age: 22.6±4.6 years) were collected. Eighty-six percent (18/21) patients presented with acute or subacute neurological disorders as their initial symptoms. The remaining fourteen percent (3/21) had psychiatric symptoms as the earliest symptoms. With progression, movement dysfunction appeared in ninety percent (19/21) of the patients with fifty-three percent (10/19) presented with weakness limited to both lower extremities. Sixty-two percent (13/21) of the patients presented with subjective sensory deficit. Seventy-one percent (15/21) had vibration sense impairment and positive Romberg's sign. Sixty-seven percent of the patients had hyporeflexia or areflexia. Fourteen percent (3/21) showed positive Babinski's sign. Seventy-eight percent (14/18) showed significantly increased homocysteine (HCY) level and only seventeen percent (3/18) showed decreased serum vitamin B12 level. T2 hyperintensity involving the posterior columns and lateral columns with inverted V sign in cervical spinal MRI had been observed in forty-seven percent (8/17) of the patients. Axonal peripheral neuropathy occurred in eighty-five percent (17/20) of the patients. The level of serum vitamin B12 and HCY, as well as imaging findings, were rapidly recovered after supplementation of Vitamin B12. Conclusion: The N2O-induced neuropsychiatric disturbances mainly occurred in the young groups and should be recognized by clinicians. The prognosis of N2O intoxication is relatively good.

18.
Ann Palliat Med ; 9(6): 3750-3756, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33302647

RESUMO

BACKGROUND: Osteoarthritis (OA) is a heterogeneous disease caused by the pathology of the synovial joint. About 10-12% of adults have symptomatic OA. In this study, the proteomics method was used to find differentially expressed proteins and to explore the material basis of traditional Chinese medicine (TCM) classification in knee OA patients. METHODS: Fifteen patients of the treatment group with knee OA of three different classifications were divided into three groups according to the dialectical classification of TCM: kidney deficiency type (5 cases), Yang deficiency type (5 cases), blood stasis type (5 cases). Also, five patients with traumatic synovitis were enrolled as the control group. The joint fluids were drawn separately. The joint fluids before treatment were Aa, Ba, and Ca groups. After one week of medication, the three joint fluid types were drawn again, and they were Ab group, Bb, and Cb groups. Liquid from the control group was named group D. Quantitative analysis of protein expression was conducted to find out the differently expressed proteins. The treatment group was treated with prescriptions for syndromes according to the TCM classification. RESULTS: The proteomics analysis identified 251 differentially expressed protein groups, and the number of groups with quantitative information for all seven channels was 246. The three treatment groups and the control group had 32 differential proteins (P<0.01), of which nine might be differential proteins between OA and traumatic synovitis. Histone H4, histone H2A, S100 calbindin A8, fibrinogen γ, fiber protein α, cDNA (FLJ92148), C4b binding protein, and partial transketolase variants were down-regulation, and basement membrane glycan was up-regulation in the treatment group compared with the control group (P<0.01). HIST1H2BC and myeloperoxidase levels in the Aa group were less than those in the D and Ab groups. The serum amyloid P and apolipoprotein CI variants in the Ba group were less than those in the D and Bb groups. Histone H2A and C-reactive protein, Fibrinogen α levels in the Ca group were less than those in the D and Cb groups. CONCLUSIONS: Nine proteins are closely related to OA of different TCM classification.

19.
Artigo em Inglês | MEDLINE | ID: mdl-33370238

RESUMO

The ultrasound (US) imaging technique has been applied to scoliosis assessment, and the proxy Cobb angle can be acquired on the US coronal images. The spinous process angle (SPA) is a valuable parameter to indicate three-dimensional (3-D) deformity of spine. However, the SPA cannot be measured on ultrasound images since the spinous process (SP) is merged in the soft tissue layer and impossible to be identified on the coronal view directly. A new method based on gradient vector flow (GVF) snake model was proposed to automatically locate SP position on the ultrasound transverse images, and the density-based spatial clustering of application with noise (DBSCAN) was used to remove the outliers out of the detected location results. With marking the SP points on the ultrasound coronal image, the SP curve was interpolated and the SPA was measured. The algorithm was evaluated on 50 subjects with various severity of scoliosis, and two raters measured the SPA on both US images and radiographs manually. The mean absolute differences (MAD) of SPAs obtained from the two modalities were 3.4±2.4° and 3.6±2.8° for two raters respectively which was less than the clinical acceptance error (5°), and the results reported a good linear correlation (r>0.85) between ultrasound method and radiography. It indicates that the proposed method can be a promising approach for SPA measurement using the ultrasound imaging technique.

20.
Artigo em Inglês | MEDLINE | ID: mdl-33373184

RESUMO

The rapid advance of fused-ring electron acceptors (FREAs) has made them a potential substitute to fullerene-based acceptors and offered new avenues for the construction of organic solar cells (OSCs). Nonfused-ring acceptors (NFRAs) could significantly reduce the synthetic cost while achieving reasonable power conversion efficiencies (PCEs). Widely used fullerene acceptors have been applied as a second acceptor to regulate the morphology, absorption, and electron transport. To take full advantage of both nonfullerene and fullerene acceptors at the same time, we rationally designed and synthesized two novel NFRAs with phenyl-C61-butyric acid methyl ester (PCBM) as the lateral pendent. With the incorporation of fullerene pendent in PCBM-C6 and PCBM-C10, varied UV-vis absorption and photoluminescence (PL) quenching behaviors were observed, and isotropic diffraction patterns were obtained via grazing incidence wide-angle X-ray scattering (GIWAXS) measurements. The bulky, spherical, and electronic isotropic fullerene pendent could effectively suppress severe molecular aggregation and form the preferred blend morphology. This strategy significantly improved the efficiencies for exciton separation and charge collection relative to the control acceptor CH3COO-C6. Finally, the Voc, Jsc, and fill factor (FF) of PCBM-C10-based devices were simultaneously improved and an enhanced PCE of 13.55% was accomplished.

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