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1.
Clin Neurophysiol ; 2021 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-33812771

RESUMO

OBJECTIVE: To investigate whether epileptiform discharge burden can identify those at risk for delayed cerebral ischemia (DCI) after subarachnoid hemorrhage (SAH). METHODS: Retrospective analysis of 113 moderate to severe grade SAH patients who had continuous EEG (cEEG) recordings during their hospitalization. We calculated the burden of epileptiform discharges (ED), measured as number of ED per hour. RESULTS: We find that many SAH patients have an increase in ED burden during the first 3-10 days following rupture, the major risk period for DCI. However, those who develop DCI have a significantly higher hourly burden from days 3.5-6 after SAH vs. those who do not. ED burden is higher in DCI patients when assessed in relation to the onset of DCI (area under the receiver operator curve 0.72). Finally, specific trends of ED burden over time, assessed by group-based trajectory analysis, also help stratify DCI risk. CONCLUSIONS: These results suggest that ED burden is a useful parameter for identifying those at higher risk of developing DCI after SAH. The higher burden rate associated with DCI supports the theory of metabolic supply-demand mismatch which contributes to this complication. SIGNIFICANCE: ED burden is a novel biomarker for predicting those at high risk of DCI.

2.
Phytomedicine ; : 153498, 2021 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-33640247

RESUMO

BACKGROUND: The incidence of nonalcoholic fatty liver disease (NAFLD), especially nonalcoholic steatohepatitis (NASH), has significantly increased in recent years and has become an important public health issue. However, no U.S. Food and Drug Administration (FDA)-approved first-line drug is currently available for the treatment of NAFLD and NASH; therefore, research on new drugs is currently a hot topic. Oroxylum indicum (Linn.) Kurz is extensively distributed in South China and South Asia and has many biological activities. However, its effects on NAFLD or even NASH and the corresponding mechanisms are still not clear. PURPOSE: To investigate the effect and mechanism of O. indicum seed extract (OISE) on preventing anti-inflammatory action in the progression from simple nonalcoholic fatty liver (NAFL) to NASH. METHODS: A network pharmacology method to construct ingredient-target networks and the protein-protein interaction (PPI) network of OISE in NASH were constructed for topological analyses and hub-target screening. Enrichment analyses were performed to identify the critical biological processes and signaling pathways. Simultaneously, in vitro and in vivo experiments investigated the effect and mechanism of OISE, baicalein, and chrysin on inflammation by biochemical indicator detection, luciferase reporters, pathological staining, and immunoblotting in oleic acid-stimulated HepG2 cells or in high-fat diet-fed rats. RESULTS: The network pharmacology showed that OISE prevented the development and progression of NAFL into NASH through various pathways and targets and that the nuclear factor NF-κB (NF-κB) pathway regulated by baicalein and chrysin played an important role in the treatment of NASH. In in vitro experiments, we further showed that OISE and its ingredients, namely, baicalein and chrysin, all improved the inflammatory status in oleic acid-stimulated HepG2 cells, inhibited the nuclear transcriptional activities of NF-κB, increased the IκB level, and decreased the phosphorylation level of NF-κB. Furthermore, in a high-fat diet-induced NASH model in rats, we also showed that OISE prevented the development and progression of NASH by inhibiting the nuclear transcriptional activity of NF-κB. CONCLUSION: OISE suppressed inflammatory responses and prevented the development and progression of NAFL into NASH through inhibition of the nuclear transcriptional activity of NF-κB. OISE may be used to treat NAFLD through many functions, including an increase in insulin sensitivity, a decrease in lipid accumulation in the liver, suppression of inflammation, and clearance of free radicals.

3.
Angle Orthod ; 90(5): 702-706, 2020 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-33378474

RESUMO

OBJECTIVES: To explore the expression of miR-34a and its effect on expression of matrix metalloproteinases (MMPs) during orthodontic tooth movement (OTM). MATERIALS AND METHODS: Twenty patients, age 12-18 years old, who underwent orthodontic treatment were enrolled. The expression of miR-34a and MMPs (MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, and MMP-14) were detected in gingival crevicular fluid by enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction at different time points. The miR-34a mimics or inhibitors were transfected into human periodontal ligament (hPDL) cells, and the MMP expression was measured by ELISA. RESULTS: The miR-34 expression in GCF on both the tension and pressure sides after orthodontic treatment were significantly downregulated, while the levels of MMPs were significantly upregulated compared with baseline level. The levels of miR-34 and MMPs returned to baseline level 3 months after orthodontic treatment. The expression of miR-34 was negatively correlated with the expression of MMP-2, MMP-9, and MMP-14. After transfection with miR-34, the MMP-2, MMP-9, and MMP-14 expression by hPDL cells were significantly downregulated compared with miR-control and miR-34 inhibitor. CONCLUSIONS: Downregulated miR-34 expression was positively correlated with MMP-2, MMP-9, and MMP-14 expression. The miR-34a transfection into hPDL cells inhibited expression of MMPs. The results suggest that miR-34a is involved in expression of MMPs during OTM.


Assuntos
Líquido do Sulco Gengival , MicroRNAs , Adolescente , Criança , Humanos , Metaloproteinase 9 da Matriz/genética , MicroRNAs/genética , Ligamento Periodontal , Técnicas de Movimentação Dentária
4.
IEEE Trans Cybern ; PP2020 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-33027022

RESUMO

The phenomenon of increasing accidents caused by reduced vigilance does exist. In the future, the high accuracy of vigilance estimation will play a significant role in public transportation safety. We propose a multimodal regression network that consists of multichannel deep autoencoders with subnetwork neurons (MCDAEsn). After we define two thresholds of ``0.35'' and ``0.70'' from the percentage of eye closure, the output values are in the continuous range of 0-0.35, 0.36-0.70, and 0.71-1 representing the awake state, the tired state, and the drowsy state, respectively. To verify the efficiency of our strategy, we first applied the proposed approach to a single modality. Then, for the multimodality, since the complementary information between forehead electrooculography and electroencephalography features, we found the performance of the proposed approach using features fusion significantly improved, demonstrating the effectiveness and efficiency of our method.

5.
3 Biotech ; 10(11): 469, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33088665

RESUMO

Industrial strains of Rhizopus oryzae is known for its strong ability to produce L-( +)-lactic acid, ethanol, and fumaric acid at high yields. To better understand the underlying mechanism behind the physiology of R. oryzae, we conducted the proteome changes between two different morphologies using two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. R. oryzae exhibited pellet morphology and filamentous morphology when the initial pH of the culture medium was 3.0 and 5.0, respectively. The concentration of lactic acid reached 63.5 g L-1 in the samples containing the pellet morphology, compared to 41.5 g L-1 produced by filamentous R. oryzae. Proteomic analysis indicated that expression levels of 128 proteins changed significantly. Of these, 17 protein spots were successfully identified by mass spectrometry and were deemed to be mainly involved in carbohydrate metabolism, genetic information processing, chitin metabolism, protein catabolism, protein folding, and antioxidative pathway. L-lactate dehydrogenase (RO3G_06188), enolase (RO3G_05466) and 2, 3-bisphosphoglycerate-independent phosphoglycerate mutase (RO3G_02462) were found to be upregulated, while isocitrate dehydrogenase (RO3G_13820) was downregulated in the samples with pellet morphology compared to the filamentous hyphae. These results suggested that more carbon flow was directed towards lactic acid biosynthesis in R. oryzae hyphae with pellet morphology.

6.
Iran J Immunol ; 17(2): 137-143, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32602467

RESUMO

BACKGROUND: IL-17 is reported to be associated with the pathophysiology of Orthodontic Tooth Movement (OTM) by affecting osteoclastogenesis. OBJECTIVE: To explore the changes of Th17 cytokines (IL-17, IL-23, and IL-27) expression and its correlation with receptor activator of nuclear factor kappa B ligand (RANKL) during orthodontic tooth movement. METHODS: Thirty patients who needed extraction of the first premolar during orthodontic treatment were included. The gingival crevicular fluid was sampled at the day of application (T0), one hour (T1), 24 hours (T2), one week (T3), four weeks (T4), and 12 weeks (T5) after the application of orthodontic force. The expression of Th17 cytokines and RANKL were measured by using enzyme-linked immunosorbent assay and, their correlations were assessed. RESULTS: The levels of IL-17A, IL-17F, IL-23, and IL-27 at both tension and pressure sides of studied teeth at T2-T4 were significantly higher compared with that of T0 and T1. Moreover, the expression of IL-27 at both tension and pressure sides of studied teeth at T2-T4 was significantly lower compared with that of T0 and T1. At T5, IL-17A, IL-17F, IL-23, and IL-27 returned to the baseline level. For the control group, the cytokines were not significantly different at various time points. The expression of IL-17A, IL-17F, and IL-23 was positively correlated with RANKL expression at T2-T4, whereas the IL-27 was negatively correlated with RANKL expression at T2-T4. CONCLUSION: This study provided preliminary evidence that Th17 cytokines may be involved in the regulation of OTM.

7.
Angle Orthod ; 2020 Mar 12.
Artigo em Inglês | MEDLINE | ID: mdl-32162969

RESUMO

OBJECTIVES: To explore the expression of miR-34a and its effect on expression of matrix metalloproteinases (MMPs) during orthodontic tooth movement (OTM). MATERIALS AND METHODS: Twenty patients, age 12-18 years old, who underwent orthodontic treatment were enrolled. The expression of miR-34a and MMPs (MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, and MMP-14) were detected in gingival crevicular fluid by enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction at different time points. The miR-34a mimics or inhibitors were transfected into human periodontal ligament (hPDL) cells, and the MMP expression was measured by ELISA. RESULTS: The miR-34 expression in GCF on both the tension and pressure sides after orthodontic treatment were significantly downregulated, while the levels of MMPs were significantly upregulated compared with baseline level. The levels of miR-34 and MMPs returned to baseline level 3 months after orthodontic treatment. The expression of miR-34 was negatively correlated with the expression of MMP-2, MMP-9, and MMP-14. After transfection with miR-34, the MMP-2, MMP-9, and MMP-14 expression by hPDL cells were significantly downregulated compared with miR-control and miR-34 inhibitor. CONCLUSIONS: Downregulated miR-34 expression was positively correlated with MMP-2, MMP-9, and MMP-14 expression. The miR-34a transfection into hPDL cells inhibited expression of MMPs. The results suggest that miR-34a is involved in expression of MMPs during OTM.

8.
Food Funct ; 11(1): 711-721, 2020 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-31909773

RESUMO

Nonalcoholic fatty liver disease (NAFLD) has become the most common liver disease worldwide; thus, a dietary supplement that can restrict hepatic fat accumulation is needed. Baicalein, a major component of Scutellaria baicalensis, is used as a dietary supplement in Eastern and Western cultures and can reduce hepatic fat accumulation. However, the detailed mechanism by which baicalein exerts this effect has yet to be elucidated in vivo and in vitro. In this study, we characterized the hepatic fat-lowering activity of baicalein and found that baicalein reduced hepatic fat accumulation by activating AMPK and suppressing SREBP1 cleavage, thus consequently inhibiting the transcriptional activity of SREBP1 and the synthesis of hepatic fat in oleic acid-induced HepG2 cells and high-fat diet-induced non-insulin-resistant mice. Moreover, baicalein improved NAFLD by decreasing TC, increasing HDLC, decreasing LDLC, affecting antioxidant activity, and exerting other effects. Therefore, the mechanism of baicalein with regard to NAFLD prevention and treatment might involve effects on multiple targets and pathways. Our study supports the use of baicalein as a dietary supplement due to its ability to reduce hepatic fat accumulation and to ameliorate NAFLD-related biochemical abnormalities.


Assuntos
Proteínas Quinases Ativadas por AMP/metabolismo , Antioxidantes/farmacologia , Flavanonas/farmacologia , Metabolismo dos Lipídeos/efeitos dos fármacos , Fígado/metabolismo , Proteína de Ligação a Elemento Regulador de Esterol 1/efeitos dos fármacos , Animais , Antioxidantes/administração & dosagem , Dieta Hiperlipídica , Flavanonas/administração & dosagem , Células Hep G2/metabolismo , Humanos , Técnicas In Vitro , Masculino , Camundongos , Camundongos Endogâmicos , Hepatopatia Gordurosa não Alcoólica/tratamento farmacológico , Ácido Oleico , Proteína de Ligação a Elemento Regulador de Esterol 1/metabolismo
9.
IEEE Trans Biomed Eng ; 67(6): 1696-1706, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-31545708

RESUMO

Sedative medications are routinely administered to provide comfort and facilitate clinical care in critically ill ICU patients. Prior work shows that brain monitoring using electroencephalography (EEG) to track sedation levels may help medical personnel to optimize drug dosing and avoid the adverse effects of oversedation and undersedation. However, the performance of sedation monitoring methods proposed to date deal poorly with individual variability across patients, leading to inconsistent performance. To address this challenge we develop an online learning approach based on Adaptive Regularization of Weight Vectors (AROW). Our approach adaptively updates a sedation level prediction algorithm under a continuously evolving data distribution. The prediction model is gradually calibrated for individual patients in response to EEG observations and routine clinical assessments over time. The evaluations are performed on a population of 172 sedated ICU patients whose sedation levels were assessed using the Richmond Agitation-Sedation Scale (scores between -5 = comatose and 0 = awake). The proposed adaptive model achieves better performance than the same model without adaptation (average accuracies with tolerance of one level difference: 68.76% vs. 61.10%). Moreover, our approach is shown to be robust to sudden changes caused by label noise. Medication administrations have different effects on model performance. We find that the model performs best in patients receiving only propofol, compared to patients receiving no sedation or multiple simultaneous sedative medications.

10.
3 Biotech ; 10(1): 14, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31879578

RESUMO

d-Phenyllactic acid is a versatile natural organic acid, which is used as an antiseptic agent, monomer of the biodegradable material poly-phenyllactic acid and in the synthesis chiral intermediate of pharmaceuticals. In this report, the novel NADH-dependent d-lactate dehydrogenase LrLDH was identified by screening a shotgun genome of Lactobacillus rossiae. To improve cofactor regeneration, the Exiguobacterium sibiricum glucose dehydrogenase EsGDH was overexpressed together with LrLDH in E. coli BL21(DE3)-pCDFDuet-1-gdh-ldh. The total enzyme activity in the fermentation broth of E. coli BL 21(DE3)-pCDFDuet-1-gdh-ldh peaked at 2359.0 U l-1 when induced by 10 g l-1 lactose at 28 °C and 150 rpm for 14 h. The biocatalytic reduction of sodium phenylpyruvate to d-phenyllactic acid was successfully carried out using whole cells of the engineered E. coli. Under the optimized biocatalysis conditions, 50 g l-1 sodium phenylpyruvate was completely converted to d-phenyllactic acid with a space-time yield and enantiomeric excess of 262.8 g l-1 day-1 and > 99.5%, respectively. To our best knowledge, it is the highest productivity reported to date, with great potential for the mass production of d-phenyllactic acid.

11.
NPJ Digit Med ; 2: 89, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31508499

RESUMO

Over- and under-sedation are common in the ICU, and contribute to poor ICU outcomes including delirium. Behavioral assessments, such as Richmond Agitation-Sedation Scale (RASS) for monitoring levels of sedation and Confusion Assessment Method for the ICU (CAM-ICU) for detecting signs of delirium, are often used. As an alternative, brain monitoring with electroencephalography (EEG) has been proposed in the operating room, but is challenging to implement in ICU due to the differences between critical illness and elective surgery, as well as the duration of sedation. Here we present a deep learning model based on a combination of convolutional and recurrent neural networks that automatically tracks both the level of consciousness and delirium using frontal EEG signals in the ICU. For level of consciousness, the system achieves a median accuracy of 70% when allowing prediction to be within one RASS level difference across all patients, which is comparable or higher than the median technician-nurse agreement at 59%. For delirium, the system achieves an AUC of 0.80 with 69% sensitivity and 83% specificity at the optimal operating point. The results show it is feasible to continuously track level of consciousness and delirium in the ICU.

12.
Annu Int Conf IEEE Eng Med Biol Soc ; 2019: 6746-6749, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31947389

RESUMO

People generally agree that emotion processing differs between male and female. However, current hypothesis of sex differences needs more objective evidence and quantitative assessment. In this paper, we investigate the sex difference in classifying ve emotions from eletroencephalograph and eye movement signals. We adopt two neural-network-based clas-si ers to objectively investigate sex differences from different perspectives. From experimental results, we nd the following three observations: (1) a general higher accuracy of same-sex strategy suggests sex-speci c factors have in uence on emotion classi cation; (2) both blink duration and frequency differ from female to male and they are negatively correlated under different emotional states; and (3) there are larger differences of brain activities in the Theta, Alpha, and Beta bands between male and female for disgust, sad, and neutral emotions.


Assuntos
Emoções , Movimentos Oculares , Eletroencefalografia , Feminino , Humanos , Masculino , Caracteres Sexuais
13.
IEEE Trans Cybern ; 49(3): 1110-1122, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29994384

RESUMO

In this paper, we present a multimodal emotion recognition framework called EmotionMeter that combines brain waves and eye movements. To increase the feasibility and wearability of EmotionMeter in real-world applications, we design a six-electrode placement above the ears to collect electroencephalography (EEG) signals. We combine EEG and eye movements for integrating the internal cognitive states and external subconscious behaviors of users to improve the recognition accuracy of EmotionMeter. The experimental results demonstrate that modality fusion with multimodal deep neural networks can significantly enhance the performance compared with a single modality, and the best mean accuracy of 85.11% is achieved for four emotions (happy, sad, fear, and neutral). We explore the complementary characteristics of EEG and eye movements for their representational capacities and identify that EEG has the advantage of classifying happy emotion, whereas eye movements outperform EEG in recognizing fear emotion. To investigate the stability of EmotionMeter over time, each subject performs the experiments three times on different days. EmotionMeter obtains a mean recognition accuracy of 72.39% across sessions with the six-electrode EEG and eye movement features. These experimental results demonstrate the effectiveness of EmotionMeter within and between sessions.


Assuntos
Aprendizado Profundo , Eletroencefalografia/métodos , Emoções/classificação , Movimentos Oculares/fisiologia , Processamento de Sinais Assistido por Computador , Adulto , Feminino , Humanos , Processamento de Imagem Assistida por Computador , Masculino , Adulto Jovem
14.
Life Sci ; 192: 270-277, 2018 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-29129771

RESUMO

Tumor metastasis leads to a poor prognosis in breast cancer, yet the mechanisms remain unclear. Docosahexaenoic acid (DHA) extracted from Antarctic krill is an optical isomer of common DHA and has a much stronger anti-neoplastic effect. In this work, the migration and invasion abilities of MCF-7 cells treated with low concentrations of Antarctic krill DHA were evaluated. Low concentrations of Antarctic krill DHA significantly reduced the numbers of migrating and invasive MCF-7 cells, whereas the cell numbers decreased slowly in the CD95-silenced MCF-7 cells, which implies that CD95 might be involved in cell migration and invasion. Additionally, co-immunoprecipitation and Western blotting demonstrated that Antarctic krill DHA induced the accumulation of CD95 and caveolin-1 interaction, resulting in the down-regulation of MMP2 expression through the FAK/SRC/PI3K/AKT signaling pathway. In conclusion, Antarctic krill DHA enhanced the interaction between CD95 and caveolin-1, which may led to an inhibitory effect on cell migration and invasion via the FAK/SRC/PI3K/AKT signaling pathway. Our study indicates that Antarctic krill DHA has great potential for tumor therapy and has revealed a new metastatic mechanism mediated by the interaction of CD95 with caveolin-1.


Assuntos
Caveolina 1/efeitos dos fármacos , Movimento Celular/efeitos dos fármacos , Ácidos Docosa-Hexaenoicos/farmacologia , Euphausiacea/química , Invasividade Neoplásica , Receptor fas/efeitos dos fármacos , Animais , Regiões Antárticas , Contagem de Células , Sobrevivência Celular , Feminino , Humanos , Células MCF-7 , Metaloproteinase 2 da Matriz/biossíntese , Metaloproteinase 2 da Matriz/genética , Metástase Neoplásica , Transdução de Sinais/efeitos dos fármacos
15.
Artigo em Inglês | MEDLINE | ID: mdl-28237087

RESUMO

Many bioactive components such as poly-unsaturated fatty acids (e.g. EPA and DHA), phospholipids and astaxanthin are known in Antarctic krill (Euphausia superba) oil. The krill DHA and EPA are generally considered to be similar to natural ones. However, two chemical compounds which were separated from Antarctic krill oil and identified as EPA and DHA by HRESIMS and NMR acted much more effective inhibitive activities on growth of several cell lines (U937, K562, SMMC-7721, PC-3, MDA-MB-231, HL60 and MCF-7) than those from sturgeon liver and commercial fish oil. Taking MCF-7 as an example, the IC50 values of Antarctic krill EPA and DHA were 14.01 and 19.94µM,while the IC50 values of sturgeon liver and commercial fish EPA and DHA were 81.45, 73.13, 82.11 and 75.31µM, respectively. Raman spectra revealed that the Antarctic krill EPA and DHA have E-configuration structures, which were different from those in commercial fish oil. Additionally, the Antarctic krill EPA and DHA had no effects on human normal liver cell line HL7702. These results indicated that the Antarctic krill E-EPA and E-DHA had a great prospect in cancer therapy.


Assuntos
Ácidos Docosa-Hexaenoicos/química , Ácidos Docosa-Hexaenoicos/farmacologia , Ácido Eicosapentaenoico/química , Ácido Eicosapentaenoico/farmacologia , Euphausiacea/química , Animais , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Óleos de Peixe/farmacologia , Humanos , Técnicas In Vitro , Células MCF-7 , Estrutura Molecular , Análise Espectral Raman
16.
J Neural Eng ; 14(2): 026017, 2017 04.
Artigo em Inglês | MEDLINE | ID: mdl-28102833

RESUMO

OBJECTIVE: Covert aspects of ongoing user mental states provide key context information for user-aware human computer interactions. In this paper, we focus on the problem of estimating the vigilance of users using EEG and EOG signals. APPROACH: The PERCLOS index as vigilance annotation is obtained from eye tracking glasses. To improve the feasibility and wearability of vigilance estimation devices for real-world applications, we adopt a novel electrode placement for forehead EOG and extract various eye movement features, which contain the principal information of traditional EOG. We explore the effects of EEG from different brain areas and combine EEG and forehead EOG to leverage their complementary characteristics for vigilance estimation. Considering that the vigilance of users is a dynamic changing process because the intrinsic mental states of users involve temporal evolution, we introduce continuous conditional neural field and continuous conditional random field models to capture dynamic temporal dependency. MAIN RESULTS: We propose a multimodal approach to estimating vigilance by combining EEG and forehead EOG and incorporating the temporal dependency of vigilance into model training. The experimental results demonstrate that modality fusion can improve the performance compared with a single modality, EOG and EEG contain complementary information for vigilance estimation, and the temporal dependency-based models can enhance the performance of vigilance estimation. From the experimental results, we observe that theta and alpha frequency activities are increased, while gamma frequency activities are decreased in drowsy states in contrast to awake states. SIGNIFICANCE: The forehead setup allows for the simultaneous collection of EEG and EOG and achieves comparative performance using only four shared electrodes in comparison with the temporal and posterior sites.


Assuntos
Algoritmos , Nível de Alerta/fisiologia , Ondas Encefálicas/fisiologia , Eletroencefalografia/métodos , Eletroculografia/métodos , Reconhecimento Automatizado de Padrão/métodos , Adulto , Feminino , Humanos , Masculino , Desempenho Psicomotor/fisiologia , Reprodutibilidade dos Testes , Sensibilidade e Especificidade
18.
Artigo em Inglês | MEDLINE | ID: mdl-25571125

RESUMO

This paper presents a new emotion recognition method which combines electroencephalograph (EEG) signals and pupillary response collected from eye tracker. We select 15 emotional film clips of 3 categories (positive, neutral and negative). The EEG signals and eye tracking data of five participants are recorded, simultaneously, while watching these videos. We extract emotion-relevant features from EEG signals and eye tracing data of 12 experiments and build a fusion model to improve the performance of emotion recognition. The best average accuracies based on EEG signals and eye tracking data are 71.77% and 58.90%, respectively. We also achieve average accuracies of 73.59% and 72.98% for feature level fusion strategy and decision level fusion strategy, respectively. These results show that both feature level fusion and decision level fusion combining EEG signals and eye tracking data can improve the performance of emotion recognition model.


Assuntos
Emoções/fisiologia , Algoritmos , Encéfalo/fisiologia , Eletroencefalografia/métodos , Movimentos Oculares , Feminino , Humanos , Iris/fisiologia , Masculino , Modelos Psicológicos , Imagem Multimodal , Reconhecimento Automatizado de Padrão , Adulto Jovem
19.
Artigo em Inglês | MEDLINE | ID: mdl-25570123

RESUMO

EEG signals, which can record the electrical activity along the scalp, provide researchers a reliable channel for investigating human emotional states. In this paper, a new algorithm, manifold regularized extreme learning machine (MRELM), is proposed for recognizing human emotional states (positive, neutral and negative) from EEG data, which were previously evoked by watching different types of movie clips. The MRELM can simultaneously consider the geometrical structure and discriminative information in EEG data. Using differential entropy features across whole five frequency bands, the average accuracy of MRELM is 81.01%, which is better than those obtained by GELM (80.25%) and SVM (76.62%). The accuracies obtained from high frequency band features (ß, γ) are obviously superior to those of low frequency band features, which shows ß and γ bands are more relevant to emotional states transition. Moreover, experiments are conducted to further evaluate the efficacy of MRELM, where the training and test sets are from different sessions. The results demonstrate that the proposed MRELM is a competitive model for EEG-based emotion recognition.


Assuntos
Algoritmos , Eletroencefalografia/métodos , Emoções/fisiologia , Aprendizado de Máquina , Reconhecimento Fisiológico de Modelo , Adulto , Feminino , Humanos , Masculino , Adulto Jovem
20.
Glycoconj J ; 29(5-6): 323-34, 2012 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-22736280

RESUMO

N-acetylglucosaminyltransferase (GnT)-IV a is a key enzyme that catalyzes the formation of the GlcNAC ß1-4 branch on the core structure of complex N-Glycans, which is the common substrate for other N-acetylglucosaminyltransferases, such as GnT-III and GnT-V. Our recent study indicates that the expression of GnT-IVa in Hca-F cells was much higher than that in Hepa1-6 cells, these two mouse hepatocarcinoma cell lines have high and no metastatic potential in lymph nodes respectively. To investigate the effects of GnT-IVa on the metastasis of hepatocarcinoma, exogenous GnT-IVa was introduced into Hepa1-6 cells, and on the other hand, the expression of GnT-IVa was down-regulated in Hca-F cells. The engineered overexpression of GnT-IVa in Hepa1-6 cells increased the antennary branches of complex N-glycans and reduced bisecting branches in vitro and in vivo, which leads to the increase in migration and metastatic capability of hepatocarcinoma cells. Conversely, down-regulated expression of GnT-IVa in Hca-F cells showed reduced tetra-antennary branches of N-Glycans, and significantly decreased the migration and metastatic capability. Furthermore, we found that the regulated GnT-IVa converts the heterogeneous N-glycosylated forms of CD147 in Hepa1-6 and Hca-F cells, and significantly changed the antennary oligosaccharide structures on CD147. These results suggest that GnT-IVa could be acting as a key role in migration and metastasis of mouse hepatocarcinoma cells through altering the glycosylation of CD147. These findings should be valuable in delineating the important function of GnT-IVa during the process of hepatocarcinoma growth and metastasis.


Assuntos
Basigina/genética , Carcinoma Hepatocelular/genética , Regulação Neoplásica da Expressão Gênica , Neoplasias Hepáticas/genética , N-Acetilglucosaminiltransferases/genética , Animais , Basigina/química , Basigina/metabolismo , Configuração de Carboidratos , Carcinoma Hepatocelular/enzimologia , Linhagem Celular Tumoral , Movimento Celular , Glicosilação , Isoenzimas/genética , Isoenzimas/metabolismo , Neoplasias Hepáticas/enzimologia , Camundongos , N-Acetilglucosaminiltransferases/metabolismo , Plasmídeos , Polissacarídeos/química , Transdução de Sinais/genética , Transfecção
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