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1.
Arthritis Rheumatol ; 2020 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-31930717

RESUMO

OBJECTIVE: GWASs have identified many susceptibility loci for systemic lupus erythematosus (SLE). However, most of these loci are located in noncoding regions of the genome. Long noncoding RNAs (lncRNAs) are pervasively expressed and reported to be involved in various diseases. This study aimed to explore the genetic significance of lncRNAs in SLE. METHODS: We performed a genome-wide survey of SLE risk variants in lncRNA gene loci among Han Chinese (4,556 SLE and 9,451 controls). The functional relevance of a risk variant in a lncRNA gene was explored using biochemical and molecular cell biology analyses. In vitro loss and gain of function strategies were performed to clarify the functional and phenotypic relevance of the susceptibility lncRNA. Moreover, correlation of this lncRNA with apoptosis were evaluated in SLE patients. RESULTS: We identified a new susceptibility locus in a lncRNA gene which we named SLEAR (rs13259960, Pcombined =1.03×10-11 , OR=1.35). The A>G variation at rs13259960, located in an intronic enhancer, impairs STAT1 recruitment to the enhancer that loops to the SLEAR promoter, resulting in decreased SLEAR level (3 G/G, 22 A/G, 103 A/A at rs13259960; P=0.0241). Moreover, SLEAR interacts with ILF2, hnRNP F and TAF15 to form a complex for transcriptional activation of the downstream anti-apoptotic genes. SLEAR regulates apoptosis in vitro and its expression level is correlated with cell death in SLE patients (n=30, r=0.824, P=2.15E-8). CONCLUSION: These findings suggest a mechanism by which the risk variant at rs13259960 modulates SLEAR expression and predisposes to SLE and may give insights into the SLE etiology.

2.
Sci Rep ; 10(1): 370, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31941951

RESUMO

Triple negative breast cancer (TNBC) is a devastating cancer disease characterized by its poor prognosis, distinct metastatic patterns, and aggressive biological behavior. Research indicates that the prevalence and presentation of TNBC varies among races, with Asian TNBC patients more commonly presenting with large invasive tumors, high node positivity, and high histologic grade. In this work, we applied ultra-high performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS)-based metabolomics to discover metabolic signatures in Asian female TNBC patients. Serum samples from 31 TNBC patients and 31 healthy controls (CN) were involved in this study. A total of 2860 metabolic features were detected in the serum samples. Among them, 77 metabolites, whose levels were significantly different between TNBC with CN, were confirmed. Using multivariate statistical analysis, literature mining, metabolic network and pathway analysis, we performed an in-depth study of the metabolic alterations in the Asian TNBC population. In addition, we discovered a panel of metabolic signatures that are highly correlated with the 5-year survival rate of the TNBC patients. This metabolomic study provides a better understanding of the metabolic details of TNBC in the Asian population.

3.
Mol Med Rep ; 21(2): 806-814, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31974621

RESUMO

Angiotensin II (Ang II) is an important bioactive peptide in the renin­angiotensin system, and it can contribute to cell proliferation and cardiac hypertrophy. Dysfunctions in transient receptor potential canonical (TRPC) channels are involved in many types of cardiovascular diseases. The aim of the present study was to investigate the role of the TRPC channel inhibitor SKF­96365 in cardiomyocyte hypertrophy induced by Ang II and the potential mechanisms of SKF­96365. H9c2 cells were treated with different concentrations of Ang II. The expression levels of cardiomyocyte hypertrophy markers and TRPC channel­related proteins were also determined. The morphology and surface area of the H9c2 cells, the expression of hypertrophic markers and TRPC channel­related proteins and the [3H] leucine incorporation rate were detected in the Ang II­treated H9c2 cells following treatment with the TRPC channel inhibitor SKF­96365. The intracellular Ca2+ concentration was tested by flow cytometry. The present results suggested that the surface area of H9c2 cells treated with Ang II was significantly increased compared with untreated H9c2 cells. The fluorescence intensity of α­actinin, the expression of hypertrophic markers and TRPC­related proteins, the [3H] leucine incorporation rate and the intracellular Ca2+ concentration were all markedly increased in the Ang II­treated H9c2 cells but decreased following SKF­96365 treatment. The present results suggested that Ang II induced cardiomyocyte hypertrophy in H9c2 cells and that the TRPC pathway may be involved in this process. Therefore, SKF­96365 can inhibit cardiomyocyte hypertrophy induced by Ang II by suppressing the TRPC pathway. The present results indicated that TRPC may be a therapeutic target for the development of novel drugs to treat cardiac hypertrophy.

4.
J Matern Fetal Neonatal Med ; : 1-8, 2019 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-31809616

RESUMO

Introduction: The efficacy of parecoxib for pain control after hysterectomy remains controversial. We conduct a systematic review and meta-analysis to explore the influence of parecoxib versus placebo on pain intensity after hysterectomy.Methods: We search PubMed, Embase, Web of Science, EBSCO, and Cochrane Library databases through March 2019 for randomized controlled trials (RCTs) assessing the effect of parecoxib versus placebo on pain intensity after hysterectomy. This meta-analysis is performed using the random-effect model.Results: Six RCTs are included in the meta-analysis. Overall, compared with control group after hysterectomy, parecoxib treatment is associated with substantially reduced pain scores in 4-6 h at rest (MD = -0.98; 95%CI = -1.14 to -0.81; p < .00001), pain scores in 12 h at rest (MD = -0.70; 95%CI = -0.77 to -0.63; p < .00001), pain scores in 12 h on sitting up (MD = -0.90; 95%CI = -1.03 to -0.77; p < .00001), pain scores in 24 h on sitting up (MD = -1.19; 95%CI = -1.94 to -0.44; p = .002), dose of analgesic need in parecoxib group is notably lower than that in control group (std. MD = -2.54; 95%CI = -3.97 to -1.10; p = .0005), but shows no obvious effect on pain scores in 24 h at rest (MD = -0.40; 95%CI = -1.47-0.67; p = .47), pain scores in 4-6 h on sitting up (MD = -0.54; 95%CI = -2.50-1.42; p = .59), first time to analgesic requirement between two groups (std. MD = -0.10; 95%CI = -0.47-0.26; p = .57), nausea or vomiting (RR = 0.92; 95%CI = 0.59-1.43; p = .70), and adverse events (RR = 0.86; 95%CI = 0.64-1.17; p = .34).Conclusions: Parecoxib treatment provides additional benefits for pain control after hysterectomy.

5.
J Autoimmun ; : 102372, 2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31810856

RESUMO

The genetic association of primary biliary cholangitis with major histocompatibility complex (MHC) has been widely confirmed among different ethnicities. To map specific MHC region variants associated with PBC in a Han Chinese cohort, we imputed HLA antigens and amino acids (AA) in 1126 PBC cases and 1770 healthy control subjects using a Han-MHC reference database. We demonstrate that HLA-DRB1 and/or HLA-DQB1 contributed the strongest signals, and that HLA-DPB1 was a separate independent locus. Regression analyses with classical HLA alleles indicate that HLA-DQB1*03:01 or HLA-DQß1-Pro55, HLA-DPB1*17:01 or HLA-DPß1-Asp84 and HLA-DRB1*08:03 could largely explain MHC association with PBC. Forward stepwise regression analyses with HLA amino acid variants localize the major signals to HLA-DRß1-Ala74, HLA-DQß1-Pro55 and HLA-DPß1-Asp84. Electrostatic potential calculations implicated AA variations at HLA-DQß1 position 55 and HLA-DPß1 position 84 as critical to peptide binding properties. Furthermore, although several critical Han Chinese AA variants differed from those shown in European populations, the predicted effects on antigen binding are likely to be very similar or identical and underlie the major component of MHC association with PBC.

6.
Sci Rep ; 9(1): 18645, 2019 Dec 09.
Artigo em Inglês | MEDLINE | ID: mdl-31819123

RESUMO

Dwarf cultivars or dwarfing rootstocks enable high-density planting and are therefore highly desirable in modern pear production. Previously, we found that the dwarf growth habit of pear is controlled by a single dominant gene PcDw. In this study, PcPIN-L (PCP021016) was cloned from dwarf-type and standard-type pears. PcPIN-L expression was significantly lower in the dwarf-type pears than in standard-type pears, which was caused by the CT repeat deletion in the promoter of dwarf-type pears. PcPIN-L overexpression in tobacco plants enhanced the growth of the stems and the roots. Notably, the indole acetic acid (IAA) content decreased in the shoot tips and increased in the stems of transgenic lines compared with wild type, which is consistent with the greater IAA content in the shoot tips and lower IAA content in the stems of dwarf-type pears than in standard-type pears. The CT repeat deletion in the promoter that causes a decrease in promoter activity is associated with lower PcPIN-L expression in the dwarf-type pears, which might limit the polar auxin transport and in turn result in the dwarf phenotype. Taken together, the results provide a novel dwarfing molecular mechanism in perennial woody plants.

7.
Artigo em Inglês | MEDLINE | ID: mdl-31868509

RESUMO

Pulmonary hypertension (PH) is a clinically common malignant cardiovascular disease. Pyroptosis is a new form of inflammatory cell death that is involved in many disease processes. Glioma-associated oncogene family zinc finger 1 (GLI1) is a transcriptional activator participated in many diseases, but its role has never been explored in inducing pyroptosis and the progress of PH. In this study, we used animal model and cell molecular biology to determine the effect of GLI1 on chronic hypoxia mediated PH progression and pulmonary artery smooth muscle cells (PASMCs) pyroptosis. The major findings of the present study are as follows: Hypoxia induced the aberrantly expressed of GLI1. The inhibition of GLI1 attenuated hypoxia-induced PH and PASMCs pyroptosis. Meanwhile, GLI1 enhanced ASC (apoptosis-associated speck-like protein containing a caspase-recruitment domain) expression by combined with its promoter. GLI1 may promote PASMCs pyroptosis through ASC to affect the progression of PH. These findings may identify novel targets for the molecular therapy of PH.

8.
Huan Jing Ke Xue ; 40(6): 2912-2919, 2019 Jun 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854686

RESUMO

The use of straw returning plus nitrogen fertilizer on farmland is one of the important agronomic practices for adjusting soil organic carbon (SOC) transformations. To explore the mechanisms of straw and nitrogen fertilizer application on straw and SOC mineralization in long-term fertilized soils, an incubation experiment with the 13C isotope tracing technique was conducted, which involved three long-term fertilized models in typical karst soils (no fertilization, inorganic fertilization, and a combination of inorganic fertilization and straw). To study the mechanisms of 13C-labeled straw and SOC mineralization, four treatments were designed as follows:no straw and nitrogen (control), and straw combined with three levels of nitrogen fertilizer (0, 214.0, and 571.0 mg·kg-1 soil). The results showed that cumulative mineralization amounts of straw-derived organic carbon in long-term fertilized soils were markedly higher than those in non-fertilized soil. Straw-derived organic carbon mineralization was significantly affected by nitrogen fertilizer levels. The positive priming effects (PE) in long-term fertilized soils were much lower than those in non-fertilized soil. The PE was decreased at the low nitrogen fertilizer level but increased at the high nitrogen fertilizer level. The principal component analysis (PCA) of phospholipid fatty acids (PLFAs) indicated that the soil microbial community structure was greatly affected by the long-term fertilization models and combined straw and nitrogen fertilizer application. Moreover, the content of PLFAs in soil microorganisms, namely, bacteria and fungi, were remarkably increased by the straw plus nitrogen fertilizer (values increased by 40.3%-53.0%, 41.1%-62.6%, and 60.5%-148.6% compared with control), but levels were not significantly affected by nitrogen fertilizer levels alone. The ratios between PLFAs of soil gram-positive and gram-negative bacteria (G+/G-) decreased and were stable at around 0.8. The structure equation models (SEM) demonstrated that the combination of straw and nitrogen affected the soil gram-positive and gram-negative bacteria structure and increased the soil DOC content, which promoted the decomposition of straw and affected the mineralization of SOC. These results indicate that straw returning plus low nitrogen fertilizer can improve the SOC sequestration capacity in karst farmland.

9.
Int J Biol Macromol ; 145: 740-749, 2019 Dec 27.
Artigo em Inglês | MEDLINE | ID: mdl-31887381

RESUMO

Russula alutacea Fr. (RaF) is a tasty mushroom with high nutritional value and have been regarded as food by local people for a long time. However, few researches have focused on the polysaccharide in RaF. In this study, a purified polysaccharide (Rap-1) was isolated with an average molecular weight of 1029.7 kDa. In vitro, Rap-1 significantly suppressed cell morphological changes and inhibited the production of nitric oxide (NO) in LPS-induced RAW 264.7 cells extracellularly and intracellularly. Besides, Rap-1 also down-regulated the expression of nuclear factor kappa-B (NF-κB), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) contents. Rap-1 ameliorated the oxidative stress and mitochondrial dysfunction caused by LPS via MAPKs signaling pathways in macrophages. In vivo, Rap-1 decreased the ROS and O2- levels and recovered the heart in zebrafish (Danio rerio) larvae induced by LPS, These results together suggested that Rap-1 could be a potential functional resource to protect against inflammatory and oxidative damage.

10.
Artigo em Inglês | MEDLINE | ID: mdl-31671680

RESUMO

The objective of this study is to examine the spillover effects of chronic diseases experienced by spouses on their wives or husbands' labour supply. Using data from 2010 and 2012 of the China Family Panel Studies (CFPS), this study employed a difference-in-difference (DD) strategy to investigate the average treatment effect of affected adults on their spouses' working hours. The results show that, after their spouses were diagnosed with chronic diseases, the average weekly working hours of wives and husbands would be significantly reduced by 3.7-4.2 h and 3.8-4.4 h, respectively. Specially, the average weekly hours of full-time work would be reduced by 2.1-3.3 h for wives and 3.6-3.8 h for husbands. The effect was stronger for those married couples with lower socioeconomic status (SES), such as low-level education, family asset, non-labour income, while the effect was insignificant for high-level SES households. Therefore, as a result of the adverse spillover effects on household labour supply, chronic diseases could cause a greater loss of labour force productivity. Additionally, households in low levels of SES may suffer more losses from reduced labour supply when spousal chronic diseases take place.

11.
Food Sci Nutr ; 7(11): 3644-3653, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31763013

RESUMO

Tetrastigma hemsleyanum is traditionally used as a folk medicine and functional food in China. Its extracts have been confirmed to have many bioactivities. However, the effect of extracting temperature on its bioactivity has not been reported. In this research, the total flavonoids content (TFC), total polyphenol content (TPC), antiproliferative, antioxidant, and anti-inflammatory activities of ethanol extracts and water extracts (extracted at 55, 70, 85, and 100°C) were observed. The results indicated that ethanol extracts showed better antioxidant activity with DPPH EC50 of 504.1 ± 3.8 µg/ml and ABTS EC50 of 851.4 ± 3.9 µg/ml. A better antiproliferative activity on HepG2, PC12, Caco-2, and Hela cells was observed on ethanol extracts. The results of anti-inflammatory activities also indicated that all of the extracts can reduce the NO production of LPS-stimulated macrophage with dose-independent manner. In summary, the results showed that the antiproliferative, antioxidant, and anti-inflammatory activities of water extracts decreased with the increasing temperature to some extent.

12.
Sci Rep ; 9(1): 17683, 2019 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-31776396

RESUMO

The complete mitochondrial genomes of Harpago chiragra and Lambis lambis (Strombidae) were determined with the size of 15,460 bp and 15,481 bp, respectively, and both sequences contained 13 protein-coding genes, 22 tRNAs, and two rRNAs. H. chiragra and L. lambis have similar mitochondrial features, corresponding to typical gastropod mitochondrial genomes, such as the conserved gene order, a high A + T content (66.22% for H. chiragra and 66.10% for L. lambis), and preference for A + T-rich codons. The start or termination codon of same protein-coding gene in H. chiragra was consistent with that in L. lambis, except for the termination codon of cox1 gene (TAG for H. chiragra and TAA for L. lambis) and the start codon of nad4 (GTG for H. chiragra and ATG for L. lambis). Pairwise sequence alignments detected different degrees of variations in H. chiragra and L. lambis mitochondrial genomes; and the two species had lower levels of genetic distance (0.202 for nucleotide sequence) and closest relationships as compared to Strombus gigas and Oncomelania hupensis. The 13 partitioned nucleotide sequences of protein coding genes of H. chiragra and L. lambis were aligned with representatives of the main lineages of gastropods and their phylogenetic relationships were inferred. H. chiragra and L. lambis share the same gene order as Littorinimorpha species, except Vermetoidea, which demonstrate a gene rearrangement in species. The reconstructed phylogeny supports three major clades within Littorinimorpha: 1) Stromboidea, Tonnoidea, Littorinoidea, and Naticoidea, 2) Rissooidea and Truncatelloidea, and 3) Vermetoidea. In addition, a relaxed molecular clock calibrated with fossils dated the diversification of Strombidae near 112 (44-206) Mya and a possible radiation is detected to occur between 45-75 Mya, providing implications to understand the Cenozoic replacement event (65-135 Mya) of Aporrhaidae by Strombidae.

13.
Int J Biol Macromol ; 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31734373

RESUMO

Immunosuppression refers to the suppression of the immune response. The immune function of immunocompromised people is not enough to resist bacterial, viral, fungal and other infections, leading to a series of diseases. A large number of experimental data show that polysaccharide compounds are immune modulators, which can enhance the body immunity with little toxic. Meanwhile, it can reduce the side effects of commonly used immunosuppressants, such as cytotoxicity, decreased ability of the body to fight infection, and inhibition of the reproduction of bone marrow hematopoietic cells. It can be used as oral or injectable drugs. In this study, a purified polysaccharide was primarily extracted from the flowers of Apios americana Medik (AAM), which can improve the immunosuppression induced by cyclophosphamide (CTX). The immunoenhancement effect of AFP was evaluated by measuring the body weight, immune organ index, cytokine secretion and antibody generated levels of CTX-induced mice. Our results showed that AFP could significantly improve the above immune indexes, which indicated AFP could alleviate immunosuppression induced by CTX. The study provided a theoretical basis for the promotion, development and application of AAM as a newly introduced food material.

14.
Int J Biol Macromol ; 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31739013

RESUMO

Ethyl Carbamate (EC), as a carcinogen widely found in fermented foods, was verified that its cytotoxicity was associated with oxidative stress. Polysaccharides from natural sources due to their antioxidative capacity have attracted great attention in the past time. In this study, purified polysaccharide from Tetrastigma hemsleyanum vines (TVP) with 64.89 kDA was extracted and conducted multiple analysis to identify its structural information. It could be discovered that TVP was composed of mannose, rhamnose, glucuronic acid, glucose, galactose, and arabinose. In vitro, TVP could inhibit cytotoxicity and genotoxicity, attenuate oxidative damage and mitochondrial dysfunction induced by EC in Caco-2 cells. Meanwhile, TVP could suppress apoptosis by mTOR and Bcl-2 signaling pathways, ameliorate oxidative via Sirt1-FoxO1 and Nrf2-Keap1 signaling pathways. In vivo, EC as well triggered the decline of survival and athletic ability in Caenorhabditis elegans (C. elegans) and TVP could reverse the decline. In the meantime, TVP could ameliorate oxidative damage in N2 and daf-2 (-) mutant but fail in daf-16 (-) mutant, which suggested that DAF-16 (FOXO) might affect the antioxidative protection of TVP in C. elegans. In brief, our results manifested that TVP could attenuate EC-induced cytotoxicity both in vitro and in vivo.

15.
Theranostics ; 9(25): 7599-7615, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31695789

RESUMO

Lipid accumulation is a driving force in tumor development, as it provides tumor cells with both energy and the building blocks of phospholipids for construction of cell membranes. Aberrant homeostasis of lipid metabolism has been observed in various tumors; however, the molecular mechanism has not been fully elucidated. Methods: Yin yang 1 (YY1) expression in hepatocellular carcinoma (HCC) was analyzed using clinical specimens, and its roles in HCC in lipid metabolism were examined using gain- and loss-of function experiments. The mechanism of YY1 regulation on peroxisome proliferator-activated receptor gamma coactivator-1ß (PGC-1ß) and its downstream genes medium-chain acyl-CoA dehydrogenase (MCAD) and long-chain acyl-CoA dehydrogenase (LCAD) were investigated using molecular biology and biochemical methods. The role of YY1/ PGC-1ß axis in hepatocarcinogenesis was studied using xenograft experiment. Results: This study showed that YY1 suppresses fatty acid ß-oxidation, leading to increase of cellular triglyceride level and lipid accumulation in HCC cells, and subsequently induction of the tumorigenesis potential of HCC cells. Molecular mechanistic study revealed that YY1 blocks the expression of PGC-1ß, an activator of fatty acid ß-oxidation, by directly binding to its promoter; and thus downregulates PGC-1ß/MCAD and PGC1-ß/LCAD axis. Importantly, we revealed that YY1 inhibition on PGC-1ß occurs irrespective of the expression of hypoxia-inducible factor-1α (HIF1-α), enabling it to promote lipid accumulation under both normoxic and hypoxic conditions. Conclusion: Our study reveals the critical role of YY1/PGC-1ß axis in HCC cell lipid metabolism, providing novel insight into the molecular mechanisms associated with tumor cell lipid metabolism, and a new perspective regarding the function of YY1 in tumor progression. Thus, our study provides evidences regarding the potential of YY1 as a target for lipid metabolism-based anti-tumor therapy.

16.
Epigenomics ; 11(14): 1613-1625, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31701765

RESUMO

Aim: To understand whether the anatomical location of origin plays a role in shaping the DNA methylation (DNAm) landscape of psoriatic skins. Patients & methods: A number of 108 psoriatic and 57 control skin samples were grouped based on their anatomical locations. Two group t-tests were used to identify those differentially methylated sites and regions. Target region methylation loci were validated by bisulfate conversion sequencing. The correlations of DNAm with pathological features, DNAm and gene expression were also interrogated. Results: Our analysis revealed 315 location-specific differentially methylated sites for back, 291 for the extremities and 801 for abdomen. Moreover, we observed that the extremity-specific loci cg21942490 located on HOXA9 is associated with hyperkeratosis. We further observed that HOXA5 and KIAA1949 are differential methylation regions. Conclusion: Our study shown evidence of anatomical location-dependent DNAm pattern in psoriasis skins, and thus provided new insights into the pathogenesis of this disease.

17.
Can J Cardiol ; 35(11): 1534-1545, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31679623

RESUMO

BACKGROUND: Pulmonary arterial hypertension (PAH) is a progressive disease, characterized by a persistent elevation of pulmonary arterial pressure and pulmonary vascular remodelling. Recent studies implicated that long noncoding RNAs (lncRNAs) play important roles in the development of various diseases. However, the underlying mechanisms of lncRNAs in PAH remain unclear. Here we show evidence for the modulation of human pulmonary smooth muscle cell (HPASMC) proliferation and vascular remodelling by lncRNA taurine upregulated gene1 (TUG1). METHODS: TUG1 expression and localization was detected by real-time polymerase chain reaction (PCR) and fluorescence in situ hybridization. Proliferation and apoptosis were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), western blot, bromodeoxyuridine incorporation, flow cytometry, scratch-wound assay, 4',6-diamidino-2-phenylindole (DAPI), and caspase-3 activity. Luciferase activity and microscale thermophoresis were used to identify biomolecular interactions. The right ventricular systolic pressure and right ventricular hypertrophy were measured to evaluate cardiopulmonary function. RESULTS: TUG1 was upregulated in the pulmonary arteries of mice after a hypoxic assault and showed a significant increase in patients with PAH. TUG1 knockdown significantly prevented the development of PAH in vivo. Moreover, TUG1 promoted the proliferative responses of HPASMCs, including cell viability, 5-bromodeoxyuridine incorporation, the expression of proliferating cell nuclear antigen, and cell-cycle progression. All these functions of TUG1 were likely to be associated with miR-328. CONCLUSIONS: The present study indicates that TUG1, a novel potential target for the treatment of PAH, is necessary for HPASMC proliferation and pulmonary vascular remodelling.

18.
Zhongguo Gu Shang ; 32(9): 833-836, 2019 Sep 25.
Artigo em Chinês | MEDLINE | ID: mdl-31615181

RESUMO

OBJECTIVE: To study the effect of soothing liver, relieving depression, invigorating spleen and reinforcing blood on reducing delirium in elderly patients with hip fracture. METHODS: From December 2014 to June 2018, 180 elderly patients with hip fracture admitted were divided into treatment group and placebo group according to the order of admission:90 patients in treatment group were treated with Jiawei Xiaoyao Powder(JWXYP), including 32 males and 58 females, with an average age of(72.12±4.92), involving 67 cases of femoral trochanter fractures and 23 cases of femoral neck fractures; 35 cases underwent dynamic hip screw fixation, 31 cases underwent intramedullary fixation and 24 cases underwent artificial hip replacement. In the placebo group, 90 patients were treated with placebo, including 37 males and 53 females, with an average age of(72.91±5.43) years old, involving 69 cases of femoral trochanteric fractures and 21 cases of femoral neck fractures, including 37 cases underwent dynamic hip screw fixation, 30 cases underwent intramedullary fixation and 23 cases underwent artificial hip replacement. The age, sex, injury site, intraoperative bleeding volume, postoperative drainage, operation time, anesthesia time, post-operative pain score, post-operative hemoglobin, post-operative CRP, delirium severity(DRS) score and delirium occurrence were observed and compared between the two groups. RESULTS: All patients were followed up until delirium returned to normal, postoperative delirium was found in 12 cases (13.33%) in the treatment group and in 39 cases(43.33%) in the placebo group, the treatment group was significantly better than the placebo group. The monitoring indexes of the two groups were compared:post-operative pain score(P=0.002), post-operative hemoglobin(P=0.012), post-operative CRP(P=0.042). CONCLUSIONS: JWXYP can relieve liver depression, invigorate spleen and invigorate blood circulation, reduce pain, inflammatory stimulation and supplement blood volume after operation, and significantly reduce the incidence of delirium after operation.


Assuntos
Delírio , Medicamentos de Ervas Chinesas/uso terapêutico , Fraturas do Quadril , Idoso , Delírio/prevenção & controle , Feminino , Fixação Interna de Fraturas , Fraturas do Quadril/cirurgia , Humanos , Masculino , Resultado do Tratamento
19.
Genet Test Mol Biomarkers ; 23(11): 791-796, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31644309

RESUMO

Objective: The purpose of this study was to investigate associations between the 14 reported loci (from a meta-analysis of genome-wide association studies [GWAS] in the Caucasian population) and vitiligo in the Chinese Han population. Materials and Methods: In this study 14 single nucleotide polymorphisms (SNPs) at 14 different genetic loci were evaluated for their association with viteligo in a Chinese Han cohort, including 1472 cases and 1472 controls of by using the Sequenom MassArray iPLEX1 system. A Bonferroni adjustment was used for multiple comparisons and pBonferroni <0.0056 was considered statistically significant. Results: The T allele of the locus within the FBXO45-NRROS gene (3q29) was significantly associated with vitiligo (odds ratio = 1.22, 95% confidence interval: 1.10-1.36, p = 0.0001). Association at the genotype level was strong (p = 0.0007). The other SNPs were not associated with vitiligo (pBonferroni >0.0056). Conclusion: A SNP at the rs6583331 locus 3q29 is associated with the susceptibility of vitiligo in the Chinese Han population, which suggests that there is a common genetic factor predisposing to the development of vitiligo in the Chinese and Caucasian populations.

20.
Aging (Albany NY) ; 11(18): 7948-7960, 2019 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-31562288

RESUMO

BACKGROUND: Cardiovascular disease (CVD) is a heavy burden on cancer patients worldwide. This study aimed to evaluate the prevalence and influence of cardiovascular risk factors (CVRF) and CVD on the all-cause mortality among Chinese cancer patients. RESULTS: Overall, 13.0% of all cancer patients had at least one type of CVRFs and 5.0% with CVDs. Patients with CVRF or CVD presented more frequently at later stages and received higher percentage of oncotherapy. During 1,782,527 person-years of follow-up, the all-cause mortality in cancer patients with CVDs and with CVRFs was higher compared with those without (182.6/1000, 109.5/1000 and 93.3/1000 person-years, respectively). Cox regression analysis showed that patients with heart failure (HR 1.79, 95% CI 1.61-1.99), myocardial infarction (HR 1.50, 95% CI 1.16-1.95), atrial fibrillation (HR 1.30, 95% CI 1.09-1.53), stroke (HR 1.21, 95% CI 1.11-1.32), hypertension (HR 1.10, 95% CI 1.04-1.16) and diabetes (HR 1.16, 95% CI 1.08-1.24) had increased all-cause mortality, whereas dyslipidemia patients had better prognosis (HR 0.73, 95% CI 0.64-0.83). Stratified by cancer type, the prognostic impact of specific CVRF or CVD varied. METHODS: We consecutively recruited 710,170 cancer patients between Feb. 1995 and Jun. 2018. A stratified Cox proportional hazards model was used to analyze the effect of comorbidities on the overall survival of patients stratified by cancer type. CONCLUSIONS: Cancer patients are vulnerable to comorbidity related to heart and cerebral disease. The influence of comorbidities on prognosis is noticeable and specific both for the type of cancer and comorbidities.

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