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1.
Medicine (Baltimore) ; 100(32): e26869, 2021 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-34397901

RESUMO

ABSTRACT: Studies have shown that rapid rehabilitation surgery has a positive effect on recovery after major orthopedic surgery. However, very few studies have examined the impact of fast track surgery on physical and psychological rehabilitation in patients who have undergone total hip replacement.This study aimed to investigate the value of the rapid rehabilitation surgical model for patients undergoing total hip arthroplasty during the perioperative period.We conducted a prospective cohort study that included patients who underwent total hip arthroplasty at our hospital from January 2015 to December 2018. We divided the patients into 2 groups - the rapid rehabilitation group and the conventional rehabilitation group - and compared their length of hospital stay, time to off-bed activity, pain score, Self-Rating Anxiety Scale scores, Self-Rating Depression Scale scores, complication rate, and rate of satisfaction during hospitalization.A total of 348 patients were included in the study. Of these, 180 received rapid rehabilitation nursing and 168 patients received conventional nursing. Compared with the patients in the conventional rehabilitation group, those in the rapid rehabilitation group had shorter hospital stays (11.5 ±â€Š1.2 day vs 15.5 ±â€Š2.3 day, P = .021), resumed off-bed activities sooner (20.5 ±â€Š3.4 hours vs 61.8 ±â€Š4.7 hours, P = .001, had less postoperative pain (4.0 ±â€Š1.2 vs 6.5 ±â€Š1.1, P < .001), and lower anxiety and depression scores (anxiety score: 24.4 ±â€Š2.1 vs 47.9 ±â€Š2.9; depression score: 25.8 ±â€Š1.8 vs 43.7 ±â€Š1.7, P < .001).The application of rapid rehabilitation surgery in total hip arthroplasty can accelerate patients' postoperative recovery, relieve anxiety and depression, and increase the patient's satisfaction with the treatment.


Assuntos
Ansiedade , Artroplastia de Quadril , Depressão , Recuperação Pós-Cirúrgica Melhorada , Complicações Pós-Operatórias/prevenção & controle , Enfermagem em Reabilitação/métodos , Idoso , Ansiedade/etiologia , Ansiedade/prevenção & controle , Artroplastia de Quadril/efeitos adversos , Artroplastia de Quadril/métodos , Artroplastia de Quadril/reabilitação , China/epidemiologia , Depressão/etiologia , Depressão/prevenção & controle , Feminino , Fraturas do Colo Femoral/epidemiologia , Fraturas do Colo Femoral/cirurgia , Necrose da Cabeça do Fêmur/epidemiologia , Necrose da Cabeça do Fêmur/cirurgia , Humanos , Tempo de Internação , Masculino , Exercício Pré-Operatório/fisiologia , Exercício Pré-Operatório/psicologia , Estudos Prospectivos , Resultado do Tratamento
2.
Acta Trop ; 223: 106087, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34389329

RESUMO

Echinococcus multilocularis metacestodes mainly reside in liver in humans and animals, and cause serious damages. UBE2N was herein shown to be downregulated in response to the infection. UBE2N was further shown to be predominantly expressed in the hepatocytes, which was also significantly downregulated during the infection. UBE2N was a target of emu-miR-4989, which was loaded into the exosomes secreted by parasites. These emu-miR-4989-encapsulating exosomes were internalized by hepatocytes, and induced a significant decrease of relative luciferase activity in the cells transfected with the construct containing a wild type of UBE2N 3'-UTR compared to the control (p < 0.05). These results demonstrate that emu-miR-4989 is involved in the UBE2N inhibition in the hepatocytes during E. multilocularis through exosomes.


Assuntos
Echinococcus multilocularis , Exossomos , MicroRNAs , Enzimas de Conjugação de Ubiquitina/genética , Animais , Equinococose , Echinococcus multilocularis/genética , Hepatócitos/parasitologia , Fígado/parasitologia , Masculino , Camundongos Endogâmicos BALB C , MicroRNAs/genética
3.
Mol Biochem Parasitol ; 245: 111408, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34343548

RESUMO

Thioredoxin peroxidase (TPx) plays an important role in protecting parasites against oxidative damage. However, studies on the role of TPxs in Echinococcus multilocularis are limited. In this study, one tpx gene of E. multilocularis, named as emtpx-1, was identified. EmTPx-1 shares two positionally conserved cysteine residues (Cys48 and Cys169) with orthologs from other platyhelminths. EmTPx-1 is highly expressed in the germinal layer and present in exosome-like vesicles secreted by E. multilocularis metacestodes. EmTPx-1 displays peroxidase activity, which removes hydrogen peroxide in the presence of dithiothreitol. Furthermore, EmTPx-1 could protect DNA from oxidative damages, and EmTPx-1-expressing E. coli cells had an enhanced resistance to oxidative stress. In addition, EmTPx-1 enhanced the expression of arg1, ym1, and il-10, but suppressed inos, tnf-α, and il-1ß expression in LPS-stimulated macrophages. Our data suggest a critical role for EmTPx-1 in oxidative stresses and M2 macrophage polarization.

4.
Mol Nutr Food Res ; 65(18): e2100380, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34318994

RESUMO

INTRODUCTION: Alzheimer's disease (AD) is a progressive neurodegeneration characterized by extensive protein aggregation and deposition in the brain, associated with defective proteasomal and autophagic-lysosomal proteolytic pathways. Since current drugs can only reduce specific symptoms, the identification of novel treatments is a major concern in AD research. Among natural compounds, (poly)phenols and their derivatives/metabolites are emerging as candidates in AD prevention due to their multiple beneficial effects. This study aims to investigate the ability of a selection of phenyl-γ-valerolactones, gut microbiota-derived metabolites of flavan-3-ols, to modulate the functionality of cellular proteolytic pathways. METHODS AND RESULTS: Neuronal SH-SY5Y cells transfected with either the wild-type or the 717 valine-to-glycine amyloid precursor protein mutated gene are used as an AD model and treated with 5-(4'-hydroxyphenyl)-γ-valerolactone, 5-(3',4'-dihydroxyphenyl)-γ-valerolactone and 5-(3'-hydroxyphenyl)-γ-valerolactone-4'-sulfate. Combining in vitro and in silico studies, it is observed that the phenyl-γ-valerolactones of interest modulated cellular proteolysis via proteasome inhibition and consequent autophagy upregulation and inhibited cathepsin B activity, eventually reducing the amount of intra- and extracellular amyloid-beta (1-42) peptides. CONCLUSION: The findings of this study establish, for the first time, that these metabolites exert a neuroprotective activity by regulating intracellular proteolysis and confirm the role of autophagy and cathepsin B as possible targets of AD preventive/therapeutic strategies.

5.
Front Immunol ; 12: 602717, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33708201

RESUMO

Echinococcosis, mainly caused by Echinococcus granulosus, is one of the 17 neglected tropical diseases. Extracellular vesicles (EVs) play an essential role in the host-parasite interplay. However, the EVs in the hydatid fluid (HF) of E. granulosus are not fully characterized. Herein, three different types of HF EVs, designated as 2 K, 10 K, and 110 K EVs based on the centrifugal force used, were morphologically identified. A total of 97, 80, and 581 proteins were identified in 2 K, 10 K, and 110 K EVs, respectively, 39 of which were commonly shared. Moreover, 11, 8, and 25 miRNAs were detected, respectively, and all of the 7 selected miRNAs were validated by qPCR to be significantly lower abundant than that in protoscoleces. It was further deemed that 110 K EVs were internalized by sheep peripheral blood mononuclear cells (PBMCs) in a time-dependent manner and thus induced interleukin (IL)-10, tumor necrosis factor-α (TNF-α), and IRF5 were significantly upregulated and IL-1ß, IL-17, and CD14 were significantly downregulated (p < 0.05). These data demonstrate the physical discrepancy of three HF EVs and an immunomodulatory effect of 110 K EVs on sheep PMBCs, suggesting a role in immune responses during E. granulosus infection.


Assuntos
Equinococose/imunologia , Echinococcus granulosus/imunologia , Vesículas Extracelulares/imunologia , Imunomodulação , Leucócitos Mononucleares/imunologia , Ovinos , Animais , Citocinas/imunologia , Equinococose/parasitologia , Proteínas de Helminto/imunologia , Ovinos/imunologia , Ovinos/parasitologia
6.
FEMS Microbiol Ecol ; 97(4)2021 03 31.
Artigo em Inglês | MEDLINE | ID: mdl-33705527

RESUMO

The gut microbiota plays an important role in human health. In modern life, with the improvement of living conditions, the intake of high-sugar and high-fat diets as well as the large-scale use of antibacterial drugs have an extensive impact on the gut microbiota, even leading to gut microbiota-orchestrating disorders. This review discusses the effects of various factors, including geographic location, age, diet, antibacterial drugs, psychological situation and exercise on gut bacteria, which helps us profoundly to understand the significance of gut bacteria to human health and to find effective solutions to prevent or treat related diseases.


Assuntos
Microbioma Gastrointestinal , Bactérias , Dieta Hiperlipídica , Humanos
7.
RNA Biol ; 18(5): 809-817, 2021 05.
Artigo em Inglês | MEDLINE | ID: mdl-33499700

RESUMO

Oncogenic viruses are associated with approximately 15% of human cancers. In viral infections, microRNAs play an important role in host-pathogen interactions. miR-21 is a highly conserved non-coding RNA that not only regulates the development of oncogenic viral diseases, but also responds to the regulation of intracellular signal pathways. Oncogenic viruses, including HBV, HCV, HPV, and EBV, co-evolve with their hosts and cause persistent infections. The upregulation of host miR-21 manipulates key cellular pathways to evade host immune responses and then promote viral replication. Thus, a better understanding of the role of miR-21 in viral infections may help us to develop effective genetically-engineered oncolytic virus-based therapies against cancer.

8.
Acta Trop ; 213: 105756, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33189712

RESUMO

Extracellular vesicles (EVs) are heterogeneous populations of different membrane-wrapped vesicles in size and encapsulated cargo and have recently emerged as a crucial carrier with the functions in intercellular communication, being involved in host-parasite interactions. However, Echinococcus granulosus EVs are not fully described. To separate EVs with a different size, the culture supernatant of E. granulosus protoscoleces (PSCs) was sequentially centrifuged at 2,000g, 10,000g and 110,000g, and the resulting precipitates were accordingly named as 2K, 10K and 110K EVs, respectively. The size and morphology of three different EVs were identified using ZETASIZER NANO and transmission electron microscopy (TEM), respectively. Then mass spectrometry was applied to define protein cargo of EVs and EV internalization was assessed using fluorescent microscopy and flow cytometry. The results showed that 2K EVs mainly ranged from 450 to 950 nm in diameter, 10K EVs ranged from 220 to 390 nm and 110K EVs from 60 to 150 nm. A total of 901 EV proteins were identified, 328 of which were commonly found in the three types of EVs. GO analysis revealed that these proteins were mainly involved in binding (44%) and catalytic activity (44%). Three types of EVs were different in biomarkers (Enolase and 14-3-3) and in reactivity with anti-echinococcosis positive serum. Moreover, 110K EVs were more easily internalized by hepatic cells than 10K EVs as well as 2K EVs (p < 0.0001). These results reveal the physical and biological discrepancy among 2K, 10K and 110K EVs, suggesting a distinct role in host-parasite interactions.


Assuntos
Equinococose/parasitologia , Echinococcus granulosus/metabolismo , Vesículas Extracelulares/metabolismo , Proteínas de Helminto/metabolismo , Interações Hospedeiro-Parasita , Animais , Transporte Biológico , Células Cultivadas , Vesículas Extracelulares/química , Citometria de Fluxo , Hepatócitos/parasitologia , Camundongos , Microscopia Eletrônica de Transmissão , Ovinos
9.
J Adv Res ; 24: 317-324, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32455006

RESUMO

Argonaute proteins are highly conserved in almost all organisms. They not only involve in the biogenesis of small regulatory RNAs, but also regulate gene expression and defend against foreign pathogen invasion via small RNA-mediated gene silencing pathways. As a key player in these pathways, the abnormal expression and/or mis-modifications of Argonaute proteins lead to the disorder of small RNA biogenesis and functions, thus influencing multiply biological processes and disease development, especially cancer. In this review, we focus on the post-translational modifications and novel functions of Argonaute proteins in alternative splicing, host defense and genome editing.

10.
Artigo em Inglês | MEDLINE | ID: mdl-32309217

RESUMO

Alveolar echinococcosis (AE) is a zoonotic helminthic disease caused by infection with the larval of Echinococcus multilocularis in human and animals. Here, we compared miRNA profiles of the peritoneal macrophages of E. multilocularis-infected and un-infected female BALB/c mice using high-throughput sequencing. A total of 87 known miRNAs were differentially expressed (fold change ≥ 2, p < 0.05) in peritoneal macrophages in mice 30- and 90-day post infection compared with ones in un-infected mice. An increase of mmu-miR-155-5p expression was observed in peritoneal macrophages in E. multilocularis-infected mice. Compared with the control group, the production of nitric oxide (NO) was increased in peritoneal macrophages transfected with mmu-miR-155-5p mimics at 12 h after transfection (p < 0.001). Two key genes (CD14 and NF-κB) in the LPS/TLR4 signaling pathway were also markedly altered in mmu-miR-155-5p mimics transfected cells (p < 0.05). Moreover, mmu-miR-155-5p mimics suppressed IL6 mRNA expression and promoted IL12a and IL12b mRNA expression. Luciferase assays showed that mmu-miR-155-5p was able to bind to the 3' UTR of the IKBKE gene and decreased luciferase activity. Finally, we found the expression of IKBKE was significantly downregulated in both macrophages transfected with mmu-miR-155-5p and macrophages isolated from E. multilocularis-infected mice. These results demonstrate an immunoregulatory effect of mmu-miR-155 on macrophages, suggesting a role in regulation of host immune responses against E. multilocularis infection.


Assuntos
Equinococose , MicroRNAs , Animais , Feminino , Perfilação da Expressão Gênica , Macrófagos Peritoneais , Camundongos , Camundongos Endogâmicos BALB C , MicroRNAs/genética
11.
Front Microbiol ; 10: 2707, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31849869

RESUMO

Alveolar echinococcosis caused by Echinococcus multilocularis is an important zoonotic disease. In the infected mice, emu-miR-4989-3p is present in sera, but its role remains unknown. Using high-throughput sequencing and qPCR, emu-miR-4989-3p was herein confirmed to be encapsulated into E. multilocularis extracellular vesicles. In the transfected macrophages, emu-miR-4989-3p was demonstrated to significantly inhibit NO production compared to the control (p < 0.05). Moreover, transfection of emu-miR-4989-3p also gave rise to the increased expression of TNF-α (p < 0.01). Furthermore, emu-miR-4989-3p induced the dysregulation of several key components in the LPS/TLR4 signaling pathway compared with the control, especially TLR4 and NF-κB that both were upregulated. Conversely, the NO production and the expression of TNF-α, TLR4 and NF-κB tended to be increased and decreased in the mimics-transfected cells upon emu-miR-4989-3p low expression, respectively. These results suggest that emu-miR-4989-3p is one of 'virulence' factors encapsulated into the extracellular vesicles, potentially playing a role in the pathogenesis of E. multilocularis.

12.
Comp Immunol Microbiol Infect Dis ; 67: 101363, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31600681

RESUMO

Exosomes, secreted by various cells, are nanometer-scale vesicles with the functions in intercellular communication. To understand a role of exosomal miRNAs in the sheeppox virus infection, exosomes were isolated from sheeppox virus-infected sheep testicular cells 0 h, 24 h and 72 h post infection. The results of transmission electron microscopy and size distribution showed that all three exosome samples were spherical particles with negatively-stained membrane, ranging from 39 nm to 127 nm in diameter. A total of 106 known and 279 novel miRNAs were identified, and 78 known and 54 novel miRNAs were commonly detected in three exosome samples. Compared with the exosomes by the uninfected controls, a total of 34 known miRNAs were aberrantly expressed in the exosomes from infected cells. In agreement with the sequencing data, the expression of oar-miR-21 and oar-miR-10b was shown to be the highest in exosomes at 24 h after SPPV-infected, and the expression of oar-let-7f was the highest in exosomes at 72 h. Conversely, the expression of oar-let-7b and oar-miR-221 was significantly decreased 24 h and 72 h post infection compared with 0 h. The analysis results also revealed that differentially expressed miRNAs were mostly involved in an immune system process and stimulus response. These results provide rich data to further investigate a role of exosomal miRNAs in SPPV-host interactions.


Assuntos
Exossomos/genética , MicroRNAs/genética , MicroRNAs/isolamento & purificação , Infecções por Poxviridae/veterinária , Animais , Capripoxvirus/genética , Masculino , Ovinos , Doenças dos Ovinos/virologia , Testículo/citologia , Testículo/virologia
13.
Infect Genet Evol ; 75: 103985, 2019 11.
Artigo em Inglês | MEDLINE | ID: mdl-31362070

RESUMO

Infection of Cysticercus tenuicollis, the larval stage of Taenia hydatigena, is extensively found in sheep and pigs and jeopardizes the breeding and meat industry. miRNAs are a subclass of small noncoding regulatory RNAs and closely associated with the pathogenesis and biology of parasites. Here, using HiSeq sequencing we identified 49 known and 2 potential novel miRNAs in C. tenuicollis, of which both thy-miR-71 and -87 were predominant. Using RT-qPCR, 6 selected miRNAs were validated, and thy-miR-71 and -miR-87 were confirmed to be highly expressed, with the copy number of approximately 82,340 ±â€¯2079 and 19,580 ±â€¯609 per 1 ng total RNA, respectively. Similar to other cestodes, T. hydatigena was predicted to have two conserved miRNA clusters thy-miR-71/2c/2b and thy-miR-4989/277, and three members of the former were confirmed to reside sequentially within the genomic region of 253 bp by PCR. The current data provide us a valuable resource for further studies of a role of miRNAs in T. hydatigena biology and infection.


Assuntos
Sequenciamento de Nucleotídeos em Larga Escala/métodos , Gado/parasitologia , MicroRNAs/genética , Taenia/genética , Animais , Cruzamento , Indústria Alimentícia , Perfilação da Expressão Gênica , Regulação da Expressão Gênica , Família Multigênica , RNA de Helmintos/genética , Reação em Cadeia da Polimerase em Tempo Real , Sequenciamento Completo do Genoma
14.
Acta Trop ; 198: 105036, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31125559

RESUMO

Taenia asiatica is an important food-borne parasite that poses a threat to food-safety and animal husbandry hygine, yet little is known about its specific infection and immune escape mechanisms. Exosome-like vesicles have recently emerged as a regulator in the interactions between parasites and hosts, providing a new direction for research on infection of T. asiatica. In this experiment, exosome-like vesicles were collected from the excretory/secretory products of cultured T. asiatica and isolated by differential centrifugation. The purified vesicles, ranging from 30 to 150 nm in size, were identified as exosome-like vesicles by transmission electron microscope and Nanoparticle tracking analysis. Proteomics analysis identified 455 proteins in the exosome-like vesicles. Of these proteins, enzymes involved in metabolic processes were identified, including glyceraldehyde 3 phosphate dehydrogenase, fructose-1, 6-bisphosphate aldolase, cytosolic malate dehydrogenase, and enolase. The two most abundant proteins from proteomic analysis, 14-3-3 and enolase, were shown to be present in the exosome-like vesicles by immunogold labeling. High-throughput RNA sequencing yielded twenty known miRNAs present in exosome-like vesicle sRNA libraries. Nine of the miRNAs, including six known miRNAs (tas-miR-71, tas-miR-1, tas-miR-7, tas-miR-9, tas-miR-10, and tas-let-7) and three newly discovered miRNAs (tas-m0022-3p, tas-m0816-3p, tas-m0082-5p), were confirmed by RT-qPCR as present in T. asiatica adult worm extracts and secreted exosome-like vesicles in T. asiatica. Additionally, we demonstrated that exosome-like vesicles experimentally labeled with PKH67 were internalized by LoVo cells in vitro. These findings provide new insights into the interaction between tapeworms and hosts mediated by exosome-like vesicles.


Assuntos
Exossomos/genética , Exossomos/metabolismo , MicroRNAs/genética , Taenia/genética , Animais , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Proteômica , Reação em Cadeia da Polimerase em Tempo Real
15.
Acta Trop ; 189: 1-5, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30248315

RESUMO

The taxonomic concept for the family Paruterinidae is controversial, especially concerning the position of the genus Cladotaenia, since the latter genus has been placed sometimes in other families, i.e. in the Taeniidae or in the distinct family Cladotaeniidae; finding a solution based on morphological data is difficult and molecular data on paruterinids and related groups are scarce. In this study, the complete mitochondrial (mt) genome sequence of the type-species of the type-genus of the Paruterinidae, Paruterina candelabraria, was determined and annotated. Gene arrangements are identical with those of Cladotaenia vulturi but differing from those of species of the family Taeniidae by the order change between tRNA-SerUCN and tRNA-LeuCUN. Phylogenetic tree was constructed by Bayesian Inference (BI) analysis using the concatenated amino acid sequences of 12 protein-coding genes. The analysis clearly shows that the Paruterinidae and Taeniidae are sister-groups, and Cladotaenia is a sister taxon of Paruterina. This supports the position of the genus Cladotaenia in the family Paruterinidae and reveals the necessity for sequencing additional taxa of the Paruterinidae for better understanding of phylogenetic relationships within the group.


Assuntos
Cestoides/classificação , Cestoides/genética , DNA Mitocondrial/análise , Genoma Mitocondrial , Sequência de Aminoácidos , Animais , Teorema de Bayes , Filogenia , RNA de Transferência/análise , Análise de Sequência de DNA
16.
Cancer Lett ; 438: 44-51, 2018 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-30219505

RESUMO

Exosomes are phospholipid bilayer membrane-enclosed vesicles in a size from 30 to 150 nm, carrying a variety of active components, such as proteins, mRNA and miRNAs, and are involved in intercellular communication. Exosomes are released by almost all living cells and detected in various biological fluids. Viruses especially oncogenic viruses have been reported to influence the formation of virus-associated cancer through reshaping the tumor microenvironment via exosomes. In this review, a role of exosomes released by oncogenic virus-infected cells in promoting or inhibiting cancer formation is outlined. Moreover, the prospects and challenges of exosome applications in cancer therapies are critically discussed.


Assuntos
Exossomos/genética , Regulação Neoplásica da Expressão Gênica , Neoplasias/genética , Microambiente Tumoral/genética , Infecções Tumorais por Vírus/genética , Comunicação Celular/genética , Exossomos/metabolismo , Exossomos/virologia , Humanos , MicroRNAs/genética , Neoplasias/metabolismo , Neoplasias/virologia , Vírus Oncogênicos/fisiologia , RNA Mensageiro/genética , Infecções Tumorais por Vírus/metabolismo , Infecções Tumorais por Vírus/virologia
17.
Exp Parasitol ; 194: 32-37, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30237048

RESUMO

Hydatigera taeniaeformis (formerly known as Taenia taeniaeformis) is a parasitic tapeworm that has a worldwide distribution. H. taeniaeformis is naturally transmitted between mice and cats and threatens to human health, especially those who are in close contact with pets. MicroRNAs (miRNAs) are a class of small regulatory non-coding RNAs involved in the regulation of parasite growth and development, parasite infection and immunology, and host-pathogen interactions. The miRNA profile of H. taeniaeformis remains to be elucidated. Herein, 47 conserved miRNAs (grouped into 34 miRNA families) and 4 novel miRNAs were identified in H. taeniaeformis metacestodes using deep sequencing approach. Among them, hta-miR-71, -let-7, and-miR-87 was absolutely predominant in H. taeniaeformis metacestodes. Moreover, comparative analysis revealed the presence of miR-71/2 and miR-4989/277 clusters in H. taeniaeformis. Nucleotide bias analysis of identified miRNAs showed that the adenine (A) was the dominant nucleotide at the beginning of the miRNAs, particularly at the positions of third and 7th nucleotides. The study provides rich data for further understandings of H. taeniaeformis biology.


Assuntos
MicroRNAs/química , MicroRNAs/isolamento & purificação , Taenia/genética , Animais , Sequência de Bases , Sequenciamento de Nucleotídeos em Larga Escala/veterinária , Humanos , Fígado/parasitologia , MicroRNAs/classificação , Família Multigênica , RNA de Helmintos/química , RNA de Helmintos/classificação , RNA de Helmintos/isolamento & purificação , Doenças dos Roedores/parasitologia , Roedores , Teníase/parasitologia , Teníase/veterinária
18.
Int Immunopharmacol ; 64: 252-255, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30212751

RESUMO

During Echinococcus multilocularis infection, serum miR-222-3p is dramatically downregulated, but its role is yet to be established. Here the expression of miR-222-3p in the spleen of infected mice was shown to be significantly decreased in response to parasite infection (p < 0.05). Using RAW264.7 macrophages, it was further demonstrated that E. multilocularis crude antigens significantly inhibited miR-222-3p expression (p < 0.01). In macrophages transfected with a miR-222-3p inhibitor, NO secretion was moderately decreased compared with the control (p < 0.05). Although all the pro- and anti-inflammatory cytokine genes tested kept constant in expression, four key genes involved in the LPS/TLR4 signaling pathway were significantly down- or up-regulated in transfected cells (p < 0.05), including CD14, TLR4, TICAM2 and AP-1. These results suggest that downregulated miR-222-3p is capable of modulating macrophage immune functions, possibly contributing to the pathogenesis during E. multilocularis infection.


Assuntos
Equinococose/etiologia , Echinococcus multilocularis , MicroRNAs/fisiologia , Animais , Equinococose/genética , Macrófagos/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Células RAW 264.7
19.
Acta Trop ; 188: 240-243, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30219554

RESUMO

Equine hosts suffer from neurological disease, congenital infection, and reproductive problems associated with Neospora spp. infection. We conducted a cross sectional study using sera from 631 equids (324 horses, 218 donkeys and 89 mules) from the southern region of Punjab province, Pakistan to determine the prevalence of antibodies against Neospora spp. in this diverse group of equines. Fisk factors associated with seropositivity were evaluated statistically based on equine type, breed, age, husbandry, breeding methods, and reproductive history. Prevalence of antibodies to Neospora spp. was detected using a commercially available competitive ELISA kit. We detected IgG antibodies to Neospora spp. in 23.3% of the equids with prevalence by host being 16.0% in horses, 32.6% in donkeys and 26.9% in 89 mules. Statistically significant (P < 0.05) differences in prevalence were observed among these hosts. Prevalence ranged in breeds of horses from 3.7% in the Morna breed, 12.4% in breeding stock, 26.8% in draughting stock, to 31.4% in unknown breeds. No significant (P > 0.05) difference in prevalence was noted among age groups. Prevalence was significantly (P < 0.05) higher in female equines with a history of abortion than those with no history of abortion. This is the first report documenting Neospora spp. in equines from Pakistan and it provides evidence that Neospora spp. is associated with abortions in female equines in Pakistan.


Assuntos
Anticorpos Antiprotozoários/sangue , Coccidiose/veterinária , Equidae/parasitologia , Neospora/imunologia , Animais , Estudos Transversais , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , Imunoglobulina G/sangue , Masculino , Paquistão/epidemiologia , Prevalência , Fatores de Risco , Estudos Soroepidemiológicos
20.
Vet Parasitol ; 255: 107-113, 2018 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-29773130

RESUMO

Tegumental proteins (TegPs) are a group of proteins that coat on the surface of worms, mainly being involved in ion uptake and immune evasion. Echinococcus species have many TegPs, but none of them have been characterized and their role remains unclear. The genome-wide analysis revealed that there were at least 14 tegp genes (tegp1 - 14) in Echinococcus species, the majority of which were found to contain an EF-hand domain or a dynein light chain-like domain or both. Despite low identity, all TegP11 proteins from 25 flatworms were conserved in structure. Echinococcus multilocularis TegP11 (emu-TegP11) was verified to be secreted by extracellular vesicles and to be localized in different spatiotemporal patterns in protoscoleces. Moreover, emu-TegP11 was also shown to have weak or no Ca2+-binding capacity. In treated macrophages, emu-TegP11 interfered with the small RNA-induced silencing pathway via inducing ectopic expression of some key component genes. Additionally, emu-TegP11 remarkably promoted NO secretion possibly by upregulation of inos gene expression (p < 0.05). It was further shown that emu-TegP11 acted as a suppressor of inflammation, with il-12B and il-1ß being significantly down-regulated (p < 0.01), and il-10 and il-4 being significantly upregulated (p < 0.05). The study demonstrates a regulatory role of emu-TegP11, likely acting as a immunomodulator to be involved in regulation of host immune system during Echinococcus infection.


Assuntos
Echinococcus granulosus/genética , Echinococcus granulosus/imunologia , Echinococcus multilocularis/genética , Echinococcus multilocularis/imunologia , Proteínas de Helminto/genética , Proteínas de Helminto/imunologia , Sequência de Aminoácidos , Animais , Proteínas de Ligação ao Cálcio/química , Proteínas de Ligação ao Cálcio/genética , Proteínas de Ligação ao Cálcio/imunologia , Proteínas de Helminto/química , Alinhamento de Sequência/veterinária
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