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1.
Immunol Lett ; 204: 60-66, 2018 12.
Artigo em Inglês | MEDLINE | ID: mdl-30321561

RESUMO

Ankylosing spondylitis (AS) is a common form of seronegative spondyloarthritis. We had identified differentially expressed genes (DEGs) in AS based on our previous RNA-sequencing results. Our study aimed to identify key genes in AS by integrated microarray analysis. In this present study, we identified 1328 DEGs between AS and normal control by using integrated analysis of two datasets derived from the Gene Expression Omnibus (GEO) database. Functional annotation of DEGs were performed. Pathways in cancer, Pancreatic cancer and Natural killer cell mediated cytotoxicity were significantly enriched pathways for DEGs. Based on the shared DEGs of AS in both integrated analysis and our previous RNA-sequencing results, we constructed the protein and protein interaction (PPI) network. BIRC2, MAPILC3A and MAGED1 were hub proteins. Validation of gene expression by qRT-PCR were performed and the results were consistent with our integrated analysis generally. Our finding provided new clues for understanding the mechanism of AS.


Assuntos
Estudos de Associação Genética , Predisposição Genética para Doença , Espondilite Anquilosante/genética , Linfócitos T CD4-Positivos/imunologia , Linfócitos T CD4-Positivos/metabolismo , Linfócitos T CD8-Positivos/imunologia , Linfócitos T CD8-Positivos/metabolismo , Estudos de Casos e Controles , Perfilação da Expressão Gênica , Estudos de Associação Genética/métodos , Humanos , Anotação de Sequência Molecular , Mapeamento de Interação de Proteínas , Mapas de Interação de Proteínas , Curva ROC , Reprodutibilidade dos Testes , Espondilite Anquilosante/imunologia , Espondilite Anquilosante/metabolismo , Transcriptoma , Fator de Necrose Tumoral alfa/metabolismo
2.
Adv Mater ; 30(21): e1706317, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29611231

RESUMO

Incorporation of N,S-codoped nanotube-like carbon (N,S-NTC) can endow electrode materials with superior electrochemical properties owing to the unique nanoarchitecture and improved kinetics. Herein, α-MnS nanoparticles (NPs) are in situ encapsulated into N,S-NTC, preparing an advanced anode material (α-MnS@N,S-NTC) for lithium-ion/sodium-ion batteries (LIBs/SIBs). It is for the first time revealed that electrochemical α → ß phase transition of MnS NPs during the 1st cycle effectively promotes Li-storage properties, which is deduced by the studies of ex situ X-ray diffraction/high-resolution transmission electron microscopy and electrode kinetics. As a result, the optimized α-MnS@N,S-NTC electrode delivers a high Li-storage capacity (1415 mA h g-1 at 50 mA g-1 ), excellent rate capability (430 mA h g-1 at 10 A g-1 ), and long-term cycling stability (no obvious capacity decay over 5000 cycles at 1 A g-1 ) with retained morphology. In addition, the N,S-NTC-based encapsulation plays the key roles on enhancing the electrochemical properties due to its high conductivity and unique 1D nanoarchitecture with excellent protective effects to active MnS NPs. Furthermore, α-MnS@N,S-NTC also delivers high Na-storage capacity (536 mA h g-1 at 50 mA g-1 ) without the occurrence of such α → ß phase transition and excellent full-cell performances as coupling with commercial LiFePO4 and LiNi0.6 Co0.2 Mn0.2 O2 cathodes in LIBs as well as Na3 V2 (PO4 )2 O2 F cathode in SIBs.

3.
Neural Regen Res ; 13(2): 304-308, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29557381

RESUMO

Electroencephalography is a sensitive indicator for measuring brain condition, and can reflect early changes in brain function and severity of cerebral ischemia. However, it is not yet known whether electroencephalography can predict development of post-cerebral infarction depression. A total of 321 patients with ischemic stroke underwent electroencephalography and Hamilton Depression Rating Scale assessment to analyze the relationship between electroencephalography and post-cerebral infarction depression. Our results show that electroencephalograms of ischemic stroke patients with depression exhibit low-amplitude alpha activity and slow theta activity. In contrast, electroencephalograms of ischemic stroke patients without depression show fast beta activity and slow delta activity. These findings confirm that low-amplitude alpha activity and slow theta activity can be considered as independent predictors for post-cerebral infarction depression.

4.
Chemistry ; 23(40): 9666-9673, 2017 Jul 18.
Artigo em Inglês | MEDLINE | ID: mdl-28508401

RESUMO

In this work, oxygen-deficient anatase TiO2 nanosheets (A-TiO2-x NSs) are proposed as a substrate to improve the electrochemical properties of sulfur electrodes for lithium-sulfur (Li-S) batteries. The A-TiO2-x NSs are prepared by partly reducing pristine TiO2 nanosheets (A-TiO2 NSs) in NaBH4 solution. With some oxygen vacancies on the surface of the TiO2 nanosheets, A-TiO2-x NSs not only promote electronic transfer, but also act as more effective polysulfide reservoirs to minimize the dissolution of lithium polysulfides (LiPSs) than the A-TiO2 NSs control. Hence, upon utilization as modifiers for cathodes of Li-S batteries, the A-TiO2-x NSs-modified sulfur (A-TiO2-x NSs-S) cathode exhibits a higher reversible specific capacity and greater cycling performance and rate capability than the A-TiO2 NSs-modified one (A-TiO2 NSs-S). For example, A-TiO2-x NSs-S delivers an initial specific capacity of 1277.1 mAh g-1 at 0.1 C and maintains a stable Coulombic efficiency of approximately 99.2 % after the first five cycles; these values are higher than those of 997.3 mAh g-1 and around 96.7 %, respectively, for A-TiO2 NSs-S. The enhanced electrochemical properties of the A-TiO2-x NSs-S cathode can be ascribed mainly to the more effective adsorption of dissolvable and diffused LiPSs by the oxygen vacancies. Therefore, utilization of the structure of oxygen vacancies in Li-S batteries demonstrates great prospects for practical application.

5.
Zhongguo Gu Shang ; 30(5): 446-452, 2017 May 25.
Artigo em Chinês | MEDLINE | ID: mdl-29417777

RESUMO

OBJECTIVE: To observe different bone cement dispersion types of PVP, PKP and manipulative reduction PVP and their effects in the treatment of senile osteoporotic vertebral compression fractures and the bone cement leakage rate. METHODS: The clinical data of patients with osteoporotic vertebral compression fractures who underwent unilateral vertebroplasty from January 2012 to January 2015 was retrospectively analyzed. Of them, 56 cases including 22 males and 34 females aged from 60 to 78 years old were treated by PVP operation; Fouty-eight cases including 17 males and 31 females aged from 61 to 79 years old were treated by PKP operation; Forty-three cases including 15 males and 28 females aged from 60 to 76 years old were treated by manipulative reduction PVP operation. AP and lateral DR films were taken after the operation; the vertebral bone cement diffusion district area and mass district area were calculated with AutoCAD graphics processing software by AP and lateral DR picture, then ratio(K) of average diffusion area and mass area were calculated, defining K<50% as mass type, 50%<=K<=100% as mixed type and K>100% as diffusion type. Different bone cement dispersion types of PVP, PKP and manipulative reduction PVP operation were analyzed. According to bone cement dispersion types, patients were divided into diffusion type, mixed type and mass type groups.Visual analogue scale (VAS), vertebral body compression rate, JOA score and bone cement leakage rate were observed. RESULTS: All patients were followed up for 12-24 months with an average of 17.2 months. There was significant difference in bone cement dispersion type among three groups (P<0.05). The constituent ratio of diffusion type, mixed type and mass type in PVP operation was 46.43%, 35.71%, 17.86%, in PKP was 16.67%, 37.50% , 45.83%, and in manipulative reduction PVP was 37.21%, 44.19% and 18.60%, respectively. PVP operation and manipulative reduction PVP were mainly composed of diffusion type and mixed type, while PKP was mainly composed of mass type and mixed type. There was no significant difference in VAS score, JOA score and bone cement leakage rate among three groups. There was statistically significant difference in postoperative vertebral body compression rate among three bone cement dispersion types(P<0.05), postoperative vertebral body compression rate in diffusion type group at 24 h postoperatively and final follow-up was (17.31±5.06)% and(18.58±4.91)%, respectively. In mixed type group, it was(14.21±5.15)% and(14.59±5.07)%, respectively. In mass type group, it was(13.89±5.02)% and(14.28±4.94)%, respectively. CONCLUSIONS: Bone cement dispersion type is different in PVP, PKP and manipulative reduction PVP operation. The bone cement dispersion of mass type and mixed type to recovery of compressed vertebral body is better than diffusion type, and there is no obvious difference in clinical effect in different bone cement dispersion type early and middle term.


Assuntos
Cimentos para Ossos/uso terapêutico , Fraturas por Compressão/cirurgia , Fraturas por Osteoporose/cirurgia , Fraturas da Coluna Vertebral/cirurgia , Vertebroplastia/métodos , Idoso , Cimentos para Ossos/efeitos adversos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Resultado do Tratamento
6.
Orthop Surg ; 8(1): 44-50, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-27028380

RESUMO

OBJECTIVE: To assess the clinical efficacy of mini-invasive transforaminal lumbar interbody fusion (TLIF) through the Wiltse approach for treating lumbar spondylolytic spondylolisthesis. METHODS: In this retrospective controlled study, 69 cases with lumbar spondylolytic spondylolisthesis treated in Qilu hospital from April to November 2014 were randomly assigned to Wiltse approach (31 cases, 16 male, 15 female; mean age 45.1 years) and traditional approach groups (38 cases, 21 male, 17 female; 47.2 years. In the Wiltse approach group, the affected level was L4, 5 in 19 cases and L5 S1 in 12, 9 of whom had low back pain (LBP) only and 21 both LBP and leg pain. There were 17 cases of I degree and 14 of II degree spondylolisthesis. Pre-operative Japanese Orthopedic Association (JOA) score was 13.1 ± 2.6; visual analog scale (VAS) for LBP 7.4 ± 1.2; VAS for leg pain 6.1 ± 2.0 and Oswestry disability index (ODI) score 42.2% ± 1.2%. In the traditional approach group, the affected level was L4, 5 in 22 cases and L5 S1 in 16, 11 of whom had LBP only and 27 both LBP and leg pain. There were 21 cases of I degree and 17 of II degree spondylolisthesis. Pre-operative JOA score was 12.8 ± 1.2; VAS for LBP 6.9 ± 1.1; VAS for leg pain 7.1 ± 2.0 and ODI score 41.2% ± 2.0%. The JOA score, VAS for LBP and leg pain, ODI dynamic X-rays, CT and/or MR were evaluated 3 and 6 months and 1 year postoperatively. RESULTS: There were no differences in sex, age, affected levels, spondylolisthesis degree, pre-operative JOA score, VAS for LBP or leg pain and ODI score between the two groups (P > 0.05). The incision length, blood loss and time to achieving exposure were better in the Wiltse approach than the traditional approach group (P < 0.05). The VAS for LBP and muscle atrophy MRI scores were significantly lower in the Wiltse approach than the traditional approach group on Days 1 and 14 and at 1 year follow-up (P < 0.05). The VAS for leg pain, JOA recovery rate and JOA and ODI scores tended to be lower in the Wiltse approach than the traditional approach group at 1 year follow-up examinations (no differences statistically significant, P > 0.05). The interbody fusion rate was not significantly different between the groups (P > 0.05). There were no complications of internal fixation in either group. CONCLUSION: TLIF via both approaches has satisfactory clinical efficacy. TLIF through the Wiltse approach significantly reduces the damage of multifidus and postoperative incidence of chronic LBP.


Assuntos
Vértebras Lombares/cirurgia , Fusão Vertebral/métodos , Espondilolistese/cirurgia , Adulto , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Estudos Retrospectivos , Resultado do Tratamento
7.
Angew Chem Int Ed Engl ; 54(34): 9907-10, 2015 Aug 17.
Artigo em Inglês | MEDLINE | ID: mdl-26140619

RESUMO

Fe/N/C is a promising non-Pt electrocatalyst for the oxygen reduction reaction (ORR), but its catalytic activity is considerably inferior to that of Pt in acidic medium, the environment of polymer electrolyte membrane fuel cells (PEMFCs). An improved Fe/N/C catalyst (denoted as Fe/N/C-SCN) derived from Fe(SCN)3, poly-m-phenylenediamine, and carbon black is presented. The advantage of using Fe(SCN)3 as iron source is that the obtained catalyst has a high level of S doping and high surface area, and thus exhibits excellent ORR activity (23 A g(-1) at 0.80 V) in 0.1 M H2SO4 solution. When the Fe/N/C-SCN was applied in a PEMFC as cathode catalyst, the maximal power density could exceed 1 W cm(-2).

8.
Eur J Pharmacol ; 762: 63-71, 2015 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-26004529

RESUMO

Pharmacological effects of solid dispersions (SDs) of a taurine zinc complex on gastric ulceration and anxiety were investigated. Pretreatment with taurine zinc (50, 100 or 200mg/kg) SDs dose-dependently protected rat gastric mucosa against cold-restraint stress (CRS)-induced gastric injury, and significantly attenuated increases in gastric mucosal H(+)K(+)-ATPase activity and lipid peroxidation and enhanced SOD activity. Taurine zinc also inhibited CRS-induced elevation of the serum stress hormones adrenocorticotropic hormone and corticosterone and upregulated HSP70 expression in the gastric mucosa. Moreover, taurine zinc (200mg/kg) SDs more potently protected the gastric mucosa from ulceration than the same dose of taurine, which may be attributed to a synergistic effect between taurine and zinc. Behavioral experiments in mice showed that taurine zinc SDs significantly increased the number of entries and time spent on the open arms in the elevated plus-maze test, time spent in the central area and total distance traveled in the open field test, and time spent and number of entries into the light compartment in the light/dark box test, indicative of reduced anxiety-like behaviors. This study demonstrates taurine zinc protected the gastric mucosa against CRS-induced gastric damage by decreasing oxidative stress, promoting endogenous HSP70 expression and attenuating psychological stress.


Assuntos
Ansiolíticos/química , Ansiolíticos/farmacologia , Temperatura Baixa/efeitos adversos , Proteínas de Choque Térmico HSP70/metabolismo , Úlcera Gástrica/tratamento farmacológico , Estresse Psicológico/complicações , Regulação para Cima/efeitos dos fármacos , Hormônio Adrenocorticotrópico/sangue , Animais , Ansiolíticos/uso terapêutico , Comportamento Animal/efeitos dos fármacos , Corticosterona/sangue , Citoproteção/efeitos dos fármacos , Escuridão , Feminino , Mucosa Gástrica/efeitos dos fármacos , Mucosa Gástrica/metabolismo , Mucosa Gástrica/patologia , Ratos , Ratos Sprague-Dawley , Restrição Física , Úlcera Gástrica/complicações , Úlcera Gástrica/metabolismo , Úlcera Gástrica/psicologia , Taurina/química , Zinco/química
9.
Environ Toxicol Pharmacol ; 39(2): 515-24, 2015 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-25681702

RESUMO

The poor bioavailability and stability of curcumin limit its clinical application. A novel Zn(II)-curcumin complex was synthesized and its effects against cyclophosphamide (CP)-induced reproductive damage were compared with curcumin. Oral administration of Zn(II)-curcumin significantly prevented CP-induced elevation of malondialdehyde (MDA) level and reductions in superoxide dismutase (SOD) activity and glutathione (GSH) content in mouse testis. Zn(II)-curcumin significantly ameliorated CP-induced reductions in body and reproductive organs weights. Zn(II)-curcumin dose-dependently ameliorated CP-induced reproductive system impairments, by improving sperm parameters (sperm count, viability, motility) and reducing serum testosterone and histological alterations. Compared to curcumin at the same dose, Zn(II)-curcumin more effectively alleviated CP-induced reproductive injury, leading to a reduced severity of testicular pathologic changes, lower MDA level, elevated SOD activity and GSH content, and increased sperm parameters and serum testosterone. These results suggest Zn(II)-curcumin more effectively protects against CP-induced reproductive damage than curcumin alone due to a synergistic reduction in oxidative stress.


Assuntos
Antioxidantes/farmacologia , Curcumina/farmacologia , Ciclofosfamida/toxicidade , Espermatozoides/efeitos dos fármacos , Testículo/efeitos dos fármacos , Zinco/farmacologia , Animais , Sobrevivência Celular/efeitos dos fármacos , Glutationa/metabolismo , Masculino , Malondialdeído/metabolismo , Camundongos , Estresse Oxidativo/efeitos dos fármacos , Contagem de Espermatozoides , Motilidade Espermática/efeitos dos fármacos , Espermatozoides/citologia , Espermatozoides/fisiologia , Superóxido Dismutase/metabolismo , Testículo/patologia , Testosterona/sangue
10.
Eur J Pharmacol ; 740: 329-36, 2014 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-25041839

RESUMO

Zinc plays a key role in maintaining gastric mucosal integrity, while alcohol dependency can lead to low zinc status. Complexes containing zinc have been reported to have better ability to protect gastric mucosa than the compounds alone. In this study, taurine zinc [Zn(NH3CH2CH2SO3)2] solid dispersions (SDs) were synthesized and investigated in an ethanol-induced ulcer model in rats. Gastric ulcer index; gastric mucosa malondialdehyde (MDA) level, glutathione (GSH) content, superoxide dismutase (SOD) activity and prostaglandin E2 (PGE2) production; and serum nitric oxide (NO) were assessed and histological analysis of the gastric mucosa tissue was performed. Taurine zinc (100, 200 mg/kg) SDs protected rat gastric mucosa from ethanol-induced injury. Moreover, the gastroprotective effect of taurine zinc SDs was accompanied by a decrease in serum NO and significant increase in gastric prostaglandin E2 (PGE2). When indomethacin, a non-selective COX inhibitor was administered before the last dose of taurine zinc, the gastroprotective effect of taurine zinc was weakened. Furthermore, taurine zinc (200 mg/kg) SDs protected against ulceration more significantly than the same dose of taurine alone, suggesting a synergistic effect between taurine and zinc. These results indicate taurine zinc protects the gastric mucosa against ethanol-induced damage by elevating antioxidants, decreasing lipid peroxidation and inhibiting the production of nitric oxide. The gastroprotective effect of taurine zinc was also partially mediated by endogenous PGE2 production.


Assuntos
Substâncias Protetoras/farmacologia , Substâncias Protetoras/uso terapêutico , Úlcera Gástrica/tratamento farmacológico , Úlcera Gástrica/metabolismo , Taurina/farmacologia , Taurina/uso terapêutico , Animais , Dinoprostona/metabolismo , Etanol , Feminino , Mucosa Gástrica/efeitos dos fármacos , Mucosa Gástrica/metabolismo , Glutationa/metabolismo , Malondialdeído/metabolismo , Óxido Nítrico/sangue , Ratos Sprague-Dawley , Úlcera Gástrica/sangue , Úlcera Gástrica/induzido quimicamente , Superóxido Dismutase/metabolismo
11.
Environ Toxicol Pharmacol ; 37(2): 729-37, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24607687

RESUMO

Curcumin can chelate metal ions, forming metallocomplexes. We compared the effects of Zn(II)-curcumin with curcumin against hemorheological alterations, oxidative stress and liver injury in a rat model of acute alcoholism. Oral administration of Zn(II)-curcumin dose-dependently prevented the ethanol-induced elevation of serum malondialdehyde (MDA) content and reductions in glutathione level and superoxide dismutase (SOD) activity. Zn(II)-curcumin also inhibited ethanol-induced liver injury. Additionally, Zn(II)-curcumin dose-dependently inhibited hemorheological abnormalities, including the ethanol-induced elevation of whole blood viscosity, plasma viscosity, blood viscosity at corrected hematocrit (45%), erythrocyte aggregation index, erythrocyte rigidity index and hematocrit. Compared to curcumin at the same dose, Zn(II)-curcumin more effectively elevated SOD activity, ameliorated liver injury and improved hemorheological variables. These results suggest that Zn(II)-curcumin protected the rats from ethanol-induced liver injury and hemorheological abnormalities via the synergistic effect of curcumin and zinc.


Assuntos
Alcoolismo/tratamento farmacológico , Curcumina/uso terapêutico , Substâncias Protetoras/uso terapêutico , Zinco/uso terapêutico , Alcoolismo/sangue , Alcoolismo/metabolismo , Alcoolismo/patologia , Animais , Viscosidade Sanguínea/efeitos dos fármacos , Curcumina/química , Curcumina/farmacologia , Modelos Animais de Doenças , Agregação Eritrocítica/efeitos dos fármacos , Etanol , Feminino , Glutationa/metabolismo , Hematócrito , Fígado/efeitos dos fármacos , Fígado/patologia , Malondialdeído/sangue , Estresse Oxidativo/efeitos dos fármacos , Substâncias Protetoras/farmacologia , Ratos Sprague-Dawley , Superóxido Dismutase/metabolismo , Zinco/química , Zinco/farmacologia , gama-Glutamiltransferase/sangue
12.
Int J Clin Exp Med ; 7(11): 4291-9, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25550944

RESUMO

This study is to investigate the effect of anterior cervical discectomy with internal fixation and fusion at different levels on adjacent segments in rabbits. Sixty New Zealand rabbits were randomly divided into four groups, one control group and three model groups, with 15 in each group. Each group underwent anterior cervical internal fixation and fusion at C3-4, C4-5, and C5-6 levels respectively. X-ray film was examined three, six and nine months after fusion to observe the changes in intervertebral space and endplate of adjacent segment. Immunohistochemistry was utilized to evaluate the effects of different fusion methods on adjacent segments of spine. As time went by, in model groups, the majority of cartilage endplates were calcified, as examined by X-ray. Immunohistochemical results of the intervertebral disc showed that the expression levels of collagen type II in nucleus pulposus were decreased significantly, while the expression levels collagen type I in annular fibrosus were increased. And collagen type I tends to replace collagen type II gradually in nucleus pulposus as time goes by. The change in collagen between upper and lower adjacent segments at C3-4 and C4-5 showed no statistical significance after fixation and fusion (p > 0.05). But for C5-6, the change showed statistical significance (p < 0.05). Cervical internal fixation and fusion can induce intervertebral disc degeneration of adjacent segment in rabbits, and cervical internal fixation and fusion operated at different levels may result in different effects on adjacent segments of cervical intervertebral disc.

13.
Food Chem Toxicol ; 60: 448-54, 2013 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-23933360

RESUMO

Gastric ulcers form as a result of a multifaceted process which includes acid secretion, reactive oxygen species generation and extracellular matrix (ECM) degradation. The aim of this study was to investigate the possible mechanisms underlying the anti-ulcerogenic effects of the Zn(II)-curcumin complex, a curcumin derivative, on the healing of acetic acid-induced gastric ulcers in rats. The severely ulcerated gastric mucosa of control animals had a lower glutathione level (GSH) and superoxide dismutase activity (SOD), and increased malondialdehyde (MDA) content compared to sham operated rats (P<0.001). Zn(II)-curcumin solid dispersions (equivalent to 12, 24 and 48 mg/kg) dose-dependently reduced the gastric ulcer index, significantly increased SOD activity and GSH levels, and reduced the MDA content and matrix metalloproteinase-9 (MMP-9) mRNA expression in the gastric mucosa (P<0.05, compared to control animals). Zn(II)-curcumin exerted a greater anti-ulcerogenic effect than curcumin at the same dose (24 mg/kg), leading to a reduced severity of gastric ulcers, lower MDA content, and increased SOD activity and GSH levels (P<0.05). In conclusion, these results confirm that the Zn(II)-curcumin complex possesses an enhanced mucosal barrier defense activity compared to curcumin alone, due to its synergistic ability to decrease oxidative stress and attenuate MMP-9-mediated inflammation.


Assuntos
Ácido Acético/efeitos adversos , Curcumina/farmacologia , Metaloproteinase 9 da Matriz/metabolismo , Úlcera Gástrica/tratamento farmacológico , Zinco/farmacologia , Animais , Modelos Animais de Doenças , Regulação para Baixo , Mucosa Gástrica/efeitos dos fármacos , Mucosa Gástrica/metabolismo , Glutationa/metabolismo , Peroxidação de Lipídeos/efeitos dos fármacos , Masculino , Malondialdeído/metabolismo , Metaloproteinase 9 da Matriz/genética , Estresse Oxidativo/efeitos dos fármacos , Ratos , Ratos Sprague-Dawley , Espécies Reativas de Oxigênio/metabolismo , Úlcera Gástrica/induzido quimicamente , Superóxido Dismutase/metabolismo , Cicatrização/efeitos dos fármacos
14.
Orthop Surg ; 3(3): 161-6, 2011 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-22009646

RESUMO

OBJECTIVE: To evaluate the clinical results of combined expansive open-door laminoplasty by splitting of spinous processes and selective anterior cervical decompression and fusion in treatment of multilevel severe cervical spondylotic myelopathy (CSM). METHODS: Twenty-eight patients (16 men and 12 women) underwent one-stage combined expansive open-door laminoplasty and selective anterior decompression and fusion for severe CSM; the average patient age was 51.3 years (range, 32-63 years). Clinical results were assessed by Japanese Orthopaedic Association (JOA) scores, number of finger grip and releases (G and R) in ten seconds, hand-grip strength, visual analog scale (VAS) of axial pain, and C2-C7 angle. RESULTS: There was no worsening of neurological symptoms due to cord injury, cerebrospinal fluid leakage, or wound infection. All cases completed one-year follow-up. The JOA scores, number of G and R in ten seconds, and hand-grip strength were all significantly improved (P < 0.05). Satisfactory decompression was shown by MRI or CT to have been achieved in all cases. The C2-C7 angle did not differ significantly from that found pre-operatively. The axial neck pain score was 2.0 ± 0.1 on VAS. CONCLUSION: Combined expansive open-door laminoplasty by splitting of spinous processes and selective anterior decompression and fusion achieves complete spinal canal decompression with minimal morbidity; this strategy is effective in improving the surgical outcomes of CSM in one-year follow-up.


Assuntos
Vértebras Cervicais/cirurgia , Descompressão Cirúrgica/métodos , Laminectomia/métodos , Compressão da Medula Espinal/cirurgia , Fusão Vertebral/métodos , Espondilose/cirurgia , Adulto , Feminino , Seguimentos , Humanos , Imagem por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Tomografia Computadorizada por Raios X , Resultado do Tratamento
15.
Am J Chin Med ; 37(3): 589-96, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19606517

RESUMO

Furanodiene is a sesquiterpene extracted from the essential oil of the rhizome of Curcuma wenyujin Y.H. Chen et C. Ling (Wen Ezhu). Furanodiene is the primary component in Wen Ezhu's essential oil, accounting for more than 20% by weight. In vitro, MTT assay was used to compare the inhibitory effects of furanodiene and Wen Ezhu's essential oil on 11 human cancer cell lines. Compared to the essential oil, furanodiene showed stronger growth inhibitions on Hela, Hep-2, HL-60, PC3, SGC-7901 and HT-1080 cells with IC(50) between 0.6-4.8 microg/ml. In vivo, furanodiene was also found to exhibit inhibitory effects on the growth of uterine cervical (U14) and sarcoma 180 (Sl80) tumors in mice. Our data suggests that furanodiene, an active component from the essential oil of Wen Ezhu, possesses efficacy against uterine cervical cancer.


Assuntos
Curcuma , Medicamentos de Ervas Chinesas/farmacologia , Furanos/farmacologia , Compostos Heterocíclicos com 2 Anéis/farmacologia , Sesquiterpenos/farmacologia , Neoplasias do Colo do Útero/tratamento farmacológico , Adenocarcinoma , Animais , Neoplasias da Mama , Carcinoma Hepatocelular , Divisão Celular/efeitos dos fármacos , Feminino , Fibrossarcoma/tratamento farmacológico , Fibrossarcoma/patologia , Células HL-60 , Células HeLa , Humanos , Células K562 , Leucemia , Neoplasias Hepáticas , Neoplasias Pulmonares , Camundongos , Camundongos Endogâmicos , Tamanho do Órgão , Baço/patologia , Timo/patologia , Neoplasias do Colo do Útero/patologia , Ensaios Antitumorais Modelo de Xenoenxerto
16.
Orthop Surg ; 1(4): 275-9, 2009 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-22009875

RESUMO

OBJECTIVE: To investigate the outcome of a laminoplasty technique without additional instrumentation for fixation of laminae in the treatment of extramedullary intradural tumors in the thoracic and lumbar spine. METHODS: Twenty-four patients (15 men and 9 women) with extramedullary intradural tumors in the thoracic and lumbar spine were included in our study. The average age was 36.2 years (range 18 to 61 years). The pathological diagnosis was neurofibroma in 13, ependymoma in 6, lipoma in 2 and teratoma in 3 cases. All patients underwent the same laminoplasty surgery as follows: the laminae were reattached to their original sites, the ligaments (including the supraspinal, interspinal and yellow ligaments) were conserved, and primary stability of the re-attached laminae was achieved with silk or nylon sutures. RESULTS: Sixty-six laminae were re-implanted in 24 patients. The average length of follow-up was 34.2 months (26.5 to 41 months). Fusion of the laminae was achieved in a mean of 4.5 months (3 to 6 months). Unilateral healing of the incision lines was observed in seven cases, including one with two-level laminotomy, three with three-level laminotomy and three with four-level laminotomy. The other 17 cases underwent bilateral fusion. CONCLUSION: Fixing the laminae, spinous processes and ligaments with sutures can achieve satisfactory primary stability and high fusion rates for resected laminae. Additional instrumentation may be necessary in greater than three-level laminoplasty.


Assuntos
Ependimoma/cirurgia , Laminectomia/métodos , Neoplasias da Coluna Vertebral/cirurgia , Adolescente , Adulto , Ependimoma/diagnóstico , Feminino , Seguimentos , Humanos , Vértebras Lombares , Imagem por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Neoplasias da Coluna Vertebral/diagnóstico , Vértebras Torácicas , Fatores de Tempo , Tomografia Computadorizada por Raios X , Resultado do Tratamento , Adulto Jovem
17.
Sleep ; 31(10): 1359-70, 2008 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-18853933

RESUMO

STUDY OBJECTIVES: To evaluate the efficacy and tolerability of gaboxadol in the treatment of adult and elderly patients with primary insomnia. DESIGN: Randomized, double-blind, placebo-controlled, multicenter, 30-night, polysomnography studies. SETTING: Sleep laboratory. PATIENTS: Primary insomnia, 18-64 y (adult study), or > or =65 y (elderly study). INTERVENTIONS: Adult study: gaboxadol 15 mg (GBX15; N = 148), 10 mg (GBX10; N = 154), or placebo (N = 156); elderly study: GBX10 (N = 157), gaboxadol 5 mg (GBX5; N = 153), or placebo (N=176). MEASUREMENTS AND RESULTS: Primary endpoints were wake after sleep onset (WASO) and latency to persistent sleep (LPS). Slow wave sleep (SWS) was a secondary endpoint. Analyses were based on the change from baseline for the average of nights 1/2, and nights 29/30, and compared gaboxadol versus placebo. Exploratory endpoints included patient's subjective assessment of total sleep time (sTST), WASO (sWASO), time to sleep onset (sTSO), and number of awakenings (sNAW); these analyses were based on weekly means. 1) Adult study. GBX15 significantly (P < or = 0.05) improved WASO through nights 29/30 but had no significant effects on LPS. No significant differences were seen for GBX10 versus placebo on WASO or LPS. GBX15 and GBX10 enhanced SWS. GBX15 significantly improved sTST, sWASO, sTSO, and sNAW at weeks 1 and 4. 2) Elderly study. GBX10 significantly improved WASO through nights 29/30; a significant improvement was also seen for GBX5 at nights 1/2 but this was not maintained through nights 29/30. GBX10 significantly improved LPS at nights 1/2 but the improvement was not maintained through nights 29/30; no significant differences were seen for GBX5 versus placebo on LPS. GBX10 and GBX5 enhanced SWS. GBX10 significantly improved sTST at week 1, and sTST, sWASO, and sNAW at week 4. Gaboxadol was generally well tolerated in both studies. CONCLUSIONS: The maximum studied doses of gaboxadol (GBX15 in adult patients and GBX10 in elderly patients) were effective at enhancing objective polysomnography measures of sleep maintenance and SWS, and also some subjective sleep measures, over 30 nights but had little or no effects on sleep onset. The clinical relevance of the enhancement of SWS by gaboxadol is unclear.


Assuntos
Agonistas GABAérgicos/uso terapêutico , Isoxazóis/uso terapêutico , Polissonografia , Distúrbios do Início e da Manutenção do Sono/tratamento farmacológico , Adolescente , Adulto , Fatores Etários , Relação Dose-Resposta a Droga , Método Duplo-Cego , Feminino , Agonistas GABAérgicos/efeitos adversos , Humanos , Isoxazóis/efeitos adversos , Masculino , Pessoa de Meia-Idade , Polissonografia/efeitos dos fármacos , Sono/efeitos dos fármacos , Adulto Jovem
18.
Transcult Psychiatry ; 40(3): 429-41, 2003 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-14649853

RESUMO

Chronic fatigue syndrome (CFS) is a relatively new condition of unknown etiology. Research suggests that psychosocial factors such as perceived social support, life stress, and acculturation may significantly influence individuals who are prone to CFS. For 57 Chinese American individuals initially diagnosed with CFS, those who recovered after one year reported lower levels of life stress than those who did not recover. Effects of changes in perceived social support also appeared to be mediated by life stress.


Assuntos
Síndrome de Fadiga Crônica/etnologia , Aculturação , Adolescente , Adulto , Idoso , China/etnologia , Síndrome de Fadiga Crônica/diagnóstico , Síndrome de Fadiga Crônica/psicologia , Feminino , Humanos , Entrevista Psicológica , Estudos Longitudinais , Masculino , Pessoa de Meia-Idade , Psicologia , Características de Residência , Apoio Social , Estresse Psicológico , Estados Unidos/epidemiologia
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