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1.
BMC Plant Biol ; 21(1): 410, 2021 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-34493227

RESUMO

BACKGROUND: Water deficit is an abiotic stress that retards plant growth and destabilizes crop production. Long non coding RNAs (lncRNAs) are a class of non-coding endogenous RNAs that participate in diverse cellular processes and stress responses in plants. lncRNAs could function as competing endogenous RNAs (ceRNA) and represent a novel layer of gene regulation. However, the regulatory mechanism of lncRNAs as ceRNA in drought stress response is yet unclear. RESULTS: In this study, we performed transcriptome-wide identification of drought-responsive lncRNAs in rice. Thereafter, we constructed a lncRNA-mediated ceRNA network by analyzing competing relationships between mRNAs and lncRNAs based on ceRNA hypothesis. A drought responsive ceRNA network with 40 lncRNAs, 23 miRNAs and 103 mRNAs was obtained. Network analysis revealed TCONS_00021861/miR528-3p/YUCCA7 regulatory axis as a hub involved in drought response. The miRNA-target expression and interaction were validated by RT-qPCR and RLM-5'RACE. TCONS_00021861 showed significant positive correlation (r = 0.7102) with YUCCA7 and negative correlation with miR528-3p (r = -0.7483). Overexpression of TCONS_00021861 attenuated the repression of miR528-3p on YUCCA7, leading to increased IAA (Indole-3-acetic acid) content and auxin overproduction phenotypes. CONCLUSIONS: TCONS_00021861 could regulate YUCCA7 by sponging miR528-3p, which in turn activates IAA biosynthetic pathway and confer resistance to drought stress. Our findings provide a new perspective of the regulatory roles of lncRNAs as ceRNAs in drought resistance of rice.


Assuntos
Oryza/genética , RNA Longo não Codificante/genética , Desidratação/genética , Secas , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Redes Reguladoras de Genes , Células do Mesofilo/ultraestrutura , MicroRNAs/genética , Folhas de Planta/genética , RNA Mensageiro/genética , RNA de Plantas , Espécies Reativas de Oxigênio/metabolismo
2.
Bioresour Technol ; 319: 124159, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33010717

RESUMO

The intrinsic recalcitrance of lignocellulosic biomass makes it resistant to enzymatic hydrolysis. The electron-rich surface of the lignin and cellulose-alike structure of hemicellulose competitively absorb the cellulase. Thus, modifying the surface charge on biomass components to alter cellulase affinity is an urgent requisite. Developing charge tunable cellulase will alter substrate affinity. Also, charge-based immobilization generates controllable substrate affinity. Within immobilized cellulase involved in situ biomass saccharification, charge effects made a crucial contribution. In addition to affecting the interaction between immobilized cellulase and biomass, charge exerts an impact on cellulase to immobilize the materials, further investigation is essential. This study aims to review the charge effects on the cellulase affinity in biomass saccharification, strategies of charge tunable cellulase, and immobilized cellulase, thereby explaining the role of electrostatic interaction. In terms of electrostatic behavior, the pathways and plans to improve in situ biomass saccharification seem to be promising.


Assuntos
Celulase , Biomassa , Celulose , Hidrólise , Lignina
3.
Plant Physiol Biochem ; 152: 184-193, 2020 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-32422535

RESUMO

Rare earth elements are known to alleviate heavy metal stress. However, the potential mechanisms of the alleviation remain unclear. This study compared the effects of La(NO3)3 and La(NO3)3-amino acid chelates (La (Ⅲ)-AA) on growth, oxidative stress, ultrastructure, bioaccumulation and gene expression in rice. Results demonstrated that 20 mg/L La (Ⅲ)-AA can effectively ameliorate CuSO4 (50 mg/L) stress in rice by reducing oxidative stress and increasing chlorophyll content, thus promoting growth. ICP and TEM revealed an antagonistic effect between La (Ⅲ) and Cu(Ⅱ). Exogenous La (Ⅲ)-AA decreased Cu(Ⅱ) content in rice leaves, stems and roots by 55.56%, 59.46% and 26.29%, and ameliorated Cu(Ⅱ) damage by maintaining the ultrastructure of mesophyll cells. RNA sequencing identified 7020 differentially expressed genes, and 8 were validated by qRT-PCR. Indole-3-acetic acid (IAA) concentration was detected by HPLC. Correlation analysis between OsGH3.4-IAA-Expansin revealed that IAA content is negatively correlated with OsGH3.4 (r = -0.82, P < 0.05), and positively correlated with Expansin (r = 0.78, P < 0.05). It's assumed that La (Ⅲ)-induced OsGH3.4 could inhibit IAA-dependent Expansin expression, thereby conferring resistance to Cu stress. This work provides novel insights into the molecular basis underlying La (Ⅲ)-induced Cu(Ⅱ) tolerance in rice.

4.
J Transl Med ; 18(1): 69, 2020 02 11.
Artigo em Inglês | MEDLINE | ID: mdl-32046742

RESUMO

BACKGROUND: Clear cell renal cell carcinoma (ccRCC) is one of the most aggressive urological malignancies. MicroRNAs (miRNAs) are post-transcriptional gene regulators in tumor pathophysiology. As miRNAs exert cooperative repressive effects on target genes, studying the miRNA synergism is important to elucidate the regulation mechanism of miRNAs. METHODS: We first created a miRNA-mRNA association network based on sequence complementarity and co-expression patterns of miRNA-targets. The synergism between miRNAs was then defined based on their expressional coherence and the concordance between target genes. The miRNA and mRNA expression were detected in RCC cell lines (786-O) using quantitative RT-PCR. Potential miRNA-target interaction was identified by Dual-Luciferase Reporter assay. Cell proliferation and migration were assessed by CCK-8 and transwell assay. RESULTS: A synergistic miRNA-miRNA interaction network of 28 miRNAs (52 miRNA pairs) with high coexpression level were constructed, among which miR-124 and miR-203 were identified as most tightly connected. ZEB2 expression is inversely correlated with miR-124 and miR-203 and verified as direct miRNA target. Cotransfection of miR-124 and miR-203 into 786-O cell lines effectively attenuated ZEB2 level and normalized renal cancer cell proliferation and migration. The inhibitory effects were abolished by ZEB2 knockdown. Furthermore, pathway analysis suggested that miR-124 and miR-203 participated in activation of epithelial-to-mesenchymal transition (EMT) pathway via regulation of ZEB2. CONCLUSIONS: Our findings provided insights into the role of miRNA-miRNA collaboration as well as a novel therapeutic approach in ccRCC.


Assuntos
Carcinoma de Células Renais , Transição Epitelial-Mesenquimal , Neoplasias Renais , MicroRNAs , Homeobox 2 de Ligação a E-box com Dedos de Zinco , Carcinoma de Células Renais/genética , Linhagem Celular Tumoral , Regulação Neoplásica da Expressão Gênica , Humanos , Neoplasias Renais/genética , MicroRNAs/genética , Homeobox 2 de Ligação a E-box com Dedos de Zinco/genética
5.
Interface Focus ; 5(3): 20140082, 2015 Jun 06.
Artigo em Inglês | MEDLINE | ID: mdl-26052419

RESUMO

We have successfully developed a plasmon-induced artificial photosynthesis system that uses a gold nanoparticle-loaded oxide semiconductor electrode to produce useful chemical energy as hydrogen and ammonia. The most important feature of this system is that both sides of a strontium titanate single-crystal substrate are used without an electrochemical apparatus. Plasmon-induced water splitting occurred even with a minimum chemical bias of 0.23 V owing to the plasmonic effects based on the efficient oxidation of water and the use of platinum as a co-catalyst for reduction. Photocurrent measurements were performed to determine the electron transfer between the gold nanoparticles and the oxide semiconductor. The efficiency of water oxidation was determined through spectroelectrochemical experiments aimed at elucidating the electron density in the gold nanoparticles. A set-up similar to the water-splitting system was used to synthesize ammonia via nitrogen fixation using ruthenium instead of platinum as a co-catalyst.

6.
Angew Chem Int Ed Engl ; 53(39): 10350-4, 2014 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-24988943

RESUMO

A plasmon-induced water splitting system that operates under irradiation by visible light was successfully developed; the system is based on the use of both sides of the same strontium titanate (SrTiO3) single-crystal substrate. The water splitting system contains two solution chambers to separate hydrogen (H2) and oxygen (O2). To promote water splitting, a chemical bias was applied by regulating the pH values of the chambers. The quantity of H2 evolved from the surface of platinum, which was used as a reduction co-catalyst, was twice the quantity of O2 evolved from an Au-nanostructured surface. Thus, the stoichiometric evolution of H2 and O2 was clearly demonstrated. The hydrogen-evolution action spectrum closely corresponds to the plasmon resonance spectrum, indicating that the plasmon-induced charge separation at the Au/SrTiO3 interface promotes water oxidation and the subsequent reduction of a proton on the backside of the SrTiO3 substrate. The chemical bias is significantly reduced by plasmonic effects, which indicates the possibility of constructing an artificial photosynthesis system with low energy consumption.

7.
Curr Pharm Des ; 17(17): 1652-62, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21619531

RESUMO

Nicotinic acetylcholine receptors (nAChRs) are members of ligand gated ion channels (LGICs) which transduce chemical signal into electrical signal in neuron and neuromuscular junction. They are pentamerics which contain an extra-cellular domain (also known as ligand binding domain or LBD), a trans-membrane domain and a cytoplasmic domain (intra-cellular domain). Agonist binding to the extra-cellular domain invokes positive ion flux as well as action potential in neurons, muscle cells and endocrine cells whereas antagonist binding inhibits ion flux. There are various endogenous or exogenous compounds which behave as agonists or antagonists targeting nAChRs. During the last decades, the whole structure of muscle type nAChR as well as the crystal structures of acetylcholine-binding proteins (AChBPs) which are homologues of the nAChRs extra-cellular domain has been obtained. These structures, together with other studies including mutation experiments and molecular simulations, provide insights into both of the nAChR architecture and its agonist binding cavity. Our review gives detailed accounts of the recent progresses in order to gain insights into agonist selectivity for different nAChR subtypes.


Assuntos
Mutação , Antagonistas Nicotínicos/farmacologia , Cristalografia por Raios X , Modelos Moleculares , Antagonistas Nicotínicos/química , Conformação Proteica
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