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1.
J Hazard Mater ; 424(Pt C): 127596, 2021 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-34808448

RESUMO

The antibiotics pollution has currently captured increasing concerns due to its potential hazards to the environment and human health. The development of efficient and viable techniques for the removal of antibiotics is one of the research hotspots in fields of wastewater treatment and pharmaceutical industry. Although the photodegradation of antibiotics is widely studied, the evolution and toxicity of degradation intermediates have been rarely documented. Herein, Pt nanoparticles (NPs) decorated BiVO4 nanosheets (Pt/BiVO4 NSs) that exhibit excellent tetracycline (TC) photodegradation activity and stability have been prepared. Especially, the TC degradation efficiency reaches ca. 88.5% after 60 min under visible light irradiation, which is superior to most of the metal loaded two-dimensional photocatalysts reported hitherto. The excellent photocatalytic activity is attributable to the enhanced light absorption capacity and charge separation efficiency in Pt/BiVO4 NSs. h+, •O2- and •OH are the main active species for TC degradation, resulting in three possible degradation pathways. Furthermore, we first verify that TC solutions treated by Pt/BiVO4 NSs are harmless to Escherichia coli K-12 and various bacteria in natural rivers, which would not stimulate Escherichia coli to produce antibiotics resistance genes (ARGs). This work develops an environmentally friendly photodegradation strategy using Pt/BiVO4 NSs with potentials for efficient remediation of antibiotics pollution in wastewater.

2.
World J Clin Cases ; 9(27): 8008-8019, 2021 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-34621857

RESUMO

BACKGROUND: Gestational anemia is a serious public health problem that affects pregnant women worldwide. Pregnancy conditions and outcomes might be associated with the presence of gestational anemia. This study investigated the association of pregnancy characteristics with anemia, exploring the potential etiology of the disease. AIM: To assess the association of pregnancy parameters with gestational anemia. METHODS: A nested case-control study was conducted based on the Chinese Pregnant Women Cohort Study-Peking Union Medical College Project (CPWCS-PUMC). A total of 3172 women were included. Patient characteristics and gestational anemia occurrence were extracted, and univariable and multivariable logistic regression models were used to analyze the association of pregnancy parameters with gestational anemia. RESULTS: Among the 3172 women, 14.0% were anemic, 46.4% were 25-30 years of age, 21.9% resided in eastern, 15.7% in middle, 12.4% in western 18.0% in southern and 32.0% in northern regions of China. Most women (65.0%) had a normal prepregnancy body mass index. Multivariable analysis found that the occurrence of gestational anemia was lower in the middle and western regions than that in the eastern region [odds ratio (OR) = 0.406, 95% confidence interval (CI): 0.309-0.533, P < 0.001)], higher in the northern than in the southern region (OR = 7.169, 95%CI: 5.139-10.003, P < 0.001), lower in full-term than in premature births (OR = 0.491, 95%CI: 0.316-0.763, P = 0.002), and higher in cases with premature membrane rupture (OR=1.404, 95%CI: 1.051-1.876, P = 0.02). CONCLUSION: Gestational anemia continues to be a health problem in China, and geographical factors may contribute to the situation. Premature birth and premature membrane rupture may be associated with gestational anemia. Therefore, we should vigorously promote local policy reformation to adapt to the demographic characteristics of at-risk pregnant women, which would potentially reduce the occurrence of gestational anemia.

3.
ACS Appl Mater Interfaces ; 13(27): 31527-31541, 2021 Jul 14.
Artigo em Inglês | MEDLINE | ID: mdl-34181398

RESUMO

It is significant to use functional biomaterials to rationally engineer microenvironments for in situ bone regeneration in the field of bone tissue engineering. To this end, we constructed the gypsum-coated ß-tricalcium phosphate (G-TCP) scaffolds by combing a three-dimensional printing technique and an epitaxial gypsum growth method. In vitro simulation experiments showed that the as-prepared scaffolds could establish a dynamic and weakly acidic microenvironment in a simulated body liquid, in which the pH and the calcium ion concentration always changed due to the gypsum degradation and growth of bone-like apatite nanoplates on the scaffold surfaces. The cell experiments confirmed that the microenvironment established by the G-TCP surfaces promoted rapid osteogenic differentiation and proliferation of bone marrow mesenchymal stem cells (BM-MSCs). In vivo experiments confirmed that the G-TCP scaffolds had high bioactivity in modulating in situ regeneration of bone, and the bioactivity of the G-TCP scaffolds was endowed by correct pore structures, degradation of gypsum, and growth of a bone-like apatite layer. The microenvironment established by the gypsum degradation could stimulate tissue inflammation and recruit white blood cells and BM-MSCs and thus accelerating native healing cascades of the bone defects via a bone growth/remodeling-absorption cycle process. Furthermore, in vivo experiments demonstrated that after the bone defects had healed completely, the as-prepared scaffolds also degraded completely within 24 weeks.


Assuntos
Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacologia , Regeneração Óssea/efeitos dos fármacos , Sulfato de Cálcio/química , Animais , Diferenciação Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Masculino , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/efeitos dos fármacos , Osteogênese/efeitos dos fármacos , Coelhos
5.
mBio ; 12(2)2021 03 23.
Artigo em Inglês | MEDLINE | ID: mdl-33758083

RESUMO

Human immunodeficiency virus type 1 (HIV-1) capsid binds host proteins during infection, including cleavage and polyadenylation specificity factor 6 (CPSF6) and cyclophilin A (CypA). We observe that HIV-1 infection induces higher-order CPSF6 formation, and capsid-CPSF6 complexes cotraffic on microtubules. CPSF6-capsid complex trafficking is impacted by capsid alterations that reduce CPSF6 binding or by excess cytoplasmic CPSF6 expression, both of which are associated with decreased HIV-1 infection. Higher-order CPSF6 complexes bind and disrupt HIV-1 capsid assemblies in vitro Disruption of HIV-1 capsid binding to CypA leads to increased CPSF6 binding and altered capsid trafficking, resulting in reduced infectivity. Our data reveal an interplay between CPSF6 and CypA that is important for cytoplasmic capsid trafficking and HIV-1 infection. We propose that CypA prevents HIV-1 capsid from prematurely engaging cytoplasmic CPSF6 and that differences in CypA cellular localization and innate immunity may explain variations in HIV-1 capsid trafficking and uncoating in CD4+ T cells and macrophages.IMPORTANCE HIV is the causative agent of AIDS, which has no cure. The protein shell that encases the viral genome, the capsid, is critical for HIV replication in cells at multiple steps. HIV capsid has been shown to interact with multiple cell proteins during movement to the cell nucleus in a poorly understood process that may differ during infection of different cell types. In this study, we show that premature or too much binding of one human protein, cleavage and polyadenylation specificity factor 6 (CPSF6), disrupts the ability of the capsid to deliver the viral genome to the cell nucleus. Another human protein, cyclophilin A (CypA), can shield HIV capsid from premature binding to CPSF6, which can differ in CD4+ T cells and macrophages. Better understanding of how HIV infects cells will allow better drugs to prevent or inhibit infection and pathogenesis.


Assuntos
Proteínas do Capsídeo/genética , Capsídeo/fisiologia , Ciclofilina A/metabolismo , HIV-1/fisiologia , Interações Hospedeiro-Patógeno , Fatores de Poliadenilação e Clivagem de mRNA/genética , Linfócitos T CD4-Positivos/virologia , Proteínas do Capsídeo/metabolismo , Núcleo Celular/metabolismo , Núcleo Celular/virologia , Células HEK293 , Células HeLa , Humanos , Imunidade Inata , Macrófagos/virologia , Replicação Viral
6.
Acta Pharmacol Sin ; 42(10): 1642-1652, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33414508

RESUMO

Vagal circuit-α7 nicotinic acetylcholine receptor (α7nAChR, coded by Chrna7) signaling can modulate lung proinflammatory responses. Arginase 1 (ARG1) plays a crucial role in the resolution of lung inflammation. However, whether vagal-α7nAChR signaling can regulate lung inflammation and ARG1 expression during an influenza infection is elusive. Here, we found that lung and spleen IL-4+ cells and lung ARG1 expression were reduced; however, bronchoalveolar lavage (BAL) protein and leukocytes and lung inflammatory cytokines were increased in PR8 (A/Puerto Rico/8/1934, H1N1)-infected vagotomized mice when compared to the control. In PR8-infected α7nAChR-deficient mice, lung Arg1, Il10, and Socs3 expression and BAL Ly6C+CD206+ cells were reduced. PR8-infected Chrna7+/+ recipient mice reconstituted with Chrna7-/- bone marrow had a lower survival as compared to PR8-infected Chrna7+/+ recipient mice reconstituted with Chrna7+/+ bone marrow. Mechanistically, the activation of α7nAChR by its agonist GTS-21 could enhance IL-4-induced Arg1 expression, reduced Nos2, and TNF-α expression in PR8-infected bone marrow-derived macrophages (BMDM). Stimulation with IL-4 increased phosphorylation of STAT6 and activation of α7nAChR increased STAT6 binding with the ARG1 promoter and relieved IL-4-induced H3K27me3 methylation by increasing JMJD3 expression in PR8-infected BMDM. Inhibition of JMJD3 increased H3K27me3 methylation and abolished α7nAChR activation and IL-4 induced ARG1 expression. Activation of α7nAChR also reduced phosphorylation of AKT1 and contained FOXO1 in the nucleus. Knockdown of Foxo1a reduced α7nAChR activation and IL-4 induced Arg1 expression in PR8-infected BMDM. Therefore, vagal-α7nAChR signaling is a novel therapeutic target for treating lung inflammatory responses during an influenza infection.

7.
Hum Cell ; 34(1): 14-27, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32920731

RESUMO

Mesenchymal stem cells (MSCs) are a promising regenerative medicine. The roles of miRNAs in osteogenic differentiation of bone marrow MSCs (BM-MSCs) remained less reported. Forkhead Box O3 (FOXO3) and alkaline phosphatase (ALP) levels in the BM-MSCs were measured on 3, 7, and 14 days after osteogenic differentiation. After transfection of FOXO3 overexpression plasmids or siFOXO3 into BM-MSCs, factors related to osteogenic differentiation or cell autophagy were determined. Besides, 3-methyladenine or rapamycin, as well as miR-223-3p mimic or inhibitor were applied to further determine the effect of FOXO3 in BM-MSCs. FOXO3 and ALP levels were increased in a time-dependent manner with osteogenic differentiation, supported by Alizarin Red Staining. Furthermore, up-regulated FOXO3 increased levels of ALP and factors related to osteogenic differentiation by increasing levels of autophagy-related factors. FOXO3, targeted by miR-223-3p, reversed the effects of miR-223-3p on factors related to BM-MSC autophagy and osteogenic differentiation. Down-regulated miR-223-3p expression promoted osteogenic differentiation of BM-MSCs by enhancing autophagy via targeting FOXO3, suggesting the potential of miR-223-3p as a therapeutic target for enhancing bone functions.


Assuntos
Autofagia/genética , Diferenciação Celular/genética , Proteína Forkhead Box O3/fisiologia , Células-Tronco Mesenquimais/fisiologia , MicroRNAs/fisiologia , Osteogênese/genética , Autofagia/fisiologia , Células Cultivadas , Humanos
8.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 28(6): 2056-2065, 2020 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-33283742

RESUMO

OBJECTIVE: To study two novel CD36 gene mutations at the CD36 splicing sites found in Guangxi population, as well as the molecular basis and population incidence of them. METHODS: DNA sequencing and cDNA clonal sequencing were used to detect CD36 exon sequence and the protein coding region sequence of CD36 mRNA for 2 CD36 deficient individuals (HHC and WGM) found in Guangxi population. Eukaryotic expression cell lines were established for the discovery of CD36 mRNA abnormal transcripts and Western blot assay was used to verify the effect of abnormal CD36 mRNA transcripts on CD36 expression. A DNA PCR-SSP genotyping method was established for the two CD36 novel mutations, and the population distribution was investigated among 110 CD36 deficient individuals in Guangxi region and 296 random individuals in Guangxi population. RESULTS: Novel mutation of c.430 -1G>C was found at the CD36 splicing site in HHC and WGM individuals, and novel mutation of c.1006 +2T>G at the CD36 splicing site was also found in the WGM individual. CD36 cDNA clonal sequencing showed that CD36 c.430 -1G>C could lead to the production of the two CD36 mRNA transcript variants: c.429_430insï¼»430-17_430-2;Cï¼½(p.Ala144fsTer1) and c.430_609del(p.Ala144_Pro203del)(GenBank:HM 217023.1); and CD36 c.1006 +2T>G could lead to the production of CD36 mRNA transcript variant of c.819_1006 del (p.Ser274GlufsTer16) (GenBank: HM217025.1). It was verified that all the three transcript variants could lead to CD36 deficiency by establishment of eukaryotic expression cell lines and Western blot assay. A study of the population incidence of two novel CD36 splicing site mutations found showed that in 110 CD36 deficient individuals and in 296 random individuals in Guangxi region, the mutation rate of CD36 c.430 -1G>C was 10.91% (12/110) and 1.35% (4/296), respectively, while CD36 c.1006 +2T>G was 2.73% (3/110) and 0 (0/296), respectively. CONCLUSION: This study identifies two novel CD36 mutations at CD36 splicing site, and preliminary clarified their molecular basis for the CD36 deficiency and the distribution characteristics in Guangxi population as well. It provides an experimental and theoretical basis for studying the molecular mechanism and characteristics of CD36 deficiency in Chinese population.


Assuntos
Transtornos Plaquetários , China , Doenças Genéticas Inatas , Humanos , Mutação , Splicing de RNA
9.
Hepatobiliary Pancreat Dis Int ; 19(4): 371-377, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32553773

RESUMO

BACKGROUND: Hypernatremic donors was regarded as the expanded criteria donors in liver transplantation. The study was to investigate the effects of donor hypernatremia on the outcomes of liver transplantation and identify the prognostic factors possibly contributing to the poor outcomes. METHODS: Donor serum sodium levels before procurement were categorized as normal sodium (< 155 mmol/L), moderate high sodium (155-170 mmol/L), and severe high sodium (≥ 170 mmol/L). Furthermore, we subdivided the 142 hypernatremic donors (≥ 155 mmol/L) into two subgroups: subgroup A, the exposure time of liver grafts from hypernatremia to reperfusion was < 36 h; and subgroup B, the exposure time was ≥ 36 h. The outcomes included initial graft function, survival rates of grafts and recipients, graft loss and early events within the first year following liver transplantation. RESULTS: There were no significant differences in the 1-year survival rates of grafts and recipients, 1-year graft loss rates and early events among the normal, moderate high and severe high sodium groups. However, the overall survival rates of grafts and recipients in subgroup A were significantly higher than those in subgroup B. Cox model showed that the exposure time (HR = 1.117; 95% CI: 1.053-1.186; P < 0.001), cold ischemia time (HR = 1.015; 95% CI: 1.006-1.024; P = 0.001) and MELD (HR = 1.061; 95% CI: 1.003-1.121; P = 0.037) were the important prognostic factors contributing to the poor outcomes of recipients with hypernatremic donors. CONCLUSIONS: The level of donor sodium immediately before organ procurement does not have negative effects on the early outcomes following adult liver transplantation. For hypernatremia liver donors, minimization of the exposure time from hypernatremia to reperfusion is critical to prevent graft loss.


Assuntos
Morte Encefálica , Sobrevivência de Enxerto , Hipernatremia/terapia , Transplante de Fígado/métodos , Doadores não Relacionados , Adulto , Feminino , Sobrevivência de Enxerto/fisiologia , Humanos , Hipernatremia/complicações , Fígado/cirurgia , Masculino , Pessoa de Meia-Idade , Preservação de Órgãos , Prognóstico , Estudos Retrospectivos , Fatores de Risco , Obtenção de Tecidos e Órgãos , Transplante Homólogo , Resultado do Tratamento
10.
J Neurosci ; 40(29): 5531-5548, 2020 07 15.
Artigo em Inglês | MEDLINE | ID: mdl-32487697

RESUMO

3-Phosphoinositide-dependent protein kinase-1 (PDK1) plays a critical role in the development of mammalian brain. Here, we investigated the role of PDK1 in Purkinje cells (PCs) by generating the PDK1-conditional knock-out mice (cKO) through crossing PV-cre or Pcp2-cre mice with Pdk1fl/fl mice. The male mice were used in the behavioral testing, and the other experiments were performed on mice of both sexes. These PDK1-cKO mice displayed decreased cerebellar size and impaired motor balance and coordination. By the electrophysiological recording, we observed the reduced spontaneous firing of PCs from the cerebellar slices of the PDK1-cKO mice. Moreover, the cell body size of PCs in the PDK1-cKO mice was time dependently reduced compared with that in the control mice. And the morphologic complexity of PCs was also decreased after PDK1 deletion. These effects may have contributed to the reduction of the rpS6 (reduced ribosomal protein S6) phosphorylation and the PKCγ expression in PDK1-cKO mice since the upregulation of pS6 by treatment of 3-benzyl-5-((2-nitrophenoxy) methyl)-dihydrofuran-2(3H)-1, the agonist of mTOR1, partly rescued the reduction in the cell body size of the PCs, and the delivery of recombinant adeno-associated virus-PKCγ through cerebellar injection rescued the reduced complexity of the dendritic arbor in PDK1-cKO mice. Together, our data suggest that PDK1, by regulating rpS6 phosphorylation and PKCγ expression, controls the cell body maintenance and the dendritic development in PCs and is critical for cerebellar motor coordination.SIGNIFICANCE STATEMENT Here, we show the role of 3-phosphoinositide-dependent protein kinase-1 (PDK1) in Purkinje cells (PCs). The ablation of PDK1 in PCs resulted in a reduction of cell body size, and dendritic complexity and abnormal spontaneous firing, which attributes to the motor defects in PDK1-conditional knock-out (cKO) mice. Moreover, the ribosomal protein S6 (rpS6) phosphorylation and the expression of PKCγ are downregulated after the ablation of PDK1. Additionally, upregulation of rpS6 phosphorylation by3-benzyl-5-((2-nitrophenoxy) methyl)-dihydrofuran-2(3H)-1 partly rescued the reduction in cell body size of PCs, and the overexpression of PKCγ in PDK1-KO PCs rescued the reduction in the dendritic complexity. These findings indicate that PDK1 contributes to the maintenance of the cell body and the dendritic development of PCs by regulating rpS6 phosphorylation and PKCγ expression.


Assuntos
Corpo Celular/fisiologia , Cerebelo/fisiologia , Dendritos/fisiologia , Células de Purkinje/fisiologia , Piruvato Desidrogenase Quinase de Transferência de Acetil/fisiologia , Transdução de Sinais , Potenciais de Ação , Animais , Comportamento Animal , Cerebelo/citologia , Cerebelo/crescimento & desenvolvimento , Feminino , Masculino , Camundongos , Camundongos Knockout , Proteína Quinase C/metabolismo , Células de Purkinje/citologia , Piruvato Desidrogenase Quinase de Transferência de Acetil/genética , Proteína S6 Ribossômica/metabolismo , Serina-Treonina Quinases TOR/metabolismo
11.
Dalton Trans ; 49(25): 8549-8556, 2020 Jul 07.
Artigo em Inglês | MEDLINE | ID: mdl-32530006

RESUMO

Herein, we illustrate a feasible strategy to strengthen the gas sensing of Y-doped CaZrO3 (YxCa1-xZr0.7O3-δ (x = 0.05, 0.06, and 0.07))/0.1Co3O4 used as sensing materials. This compound was prepared via a solid-state reaction technique. The structural, morphological, electrical, and sensing features such as phase identification, microstructure, ionic conductivity, total conductivity and sensitivity of the fabricated sensors were evaluated via X-ray diffraction, scanning electron microscopy, electron-blocking method, electrochemical impedance spectroscopy and cyclic voltammetry. In addition, the influence of the Y-dopant on the properties of YxCa1-xZr0.7O3-δ/Co3O4 was thoroughly studied. XRD results revealed the formation of the orthorhombic perovskite phase of YxCa1-xZr0.7O3-δ. Moreover, the obtained results from the electrical properties elucidated high electronic and low ionic conductivities, and small polaron conduction of YxCa1-xZr0.7O3-δ/Co3O4. Furthermore, the results confirmed an excellent limiting current plateau for the fabricated oxygen sensor based on YxCa1-xZr0.7O3-δ/Co3O4. In particular, experimental observation indicates that Y-doping at the Ca site and/or Zr site might be difficult.

12.
Dalton Trans ; 49(20): 6682-6692, 2020 May 28.
Artigo em Inglês | MEDLINE | ID: mdl-32367105

RESUMO

First-principles calculations were used to explore the effect of various Y-doping levels on the electrical conductivity of SrTiO3. Herein, we prepared ((Y0.07Sr0.93Ti0.6Fe0.4-xO3-δ)/x/3Co3O4 (x = 0.1, 0.2, 0.3)) composites using a solid state reaction method. The properties of these sensing materials and the fabricated sensors including crystal phase composition, microstructures, oxygen ionic conductivity, total conductivity and sensor performance were investigated in detail. XRD demonstrates the formation of a highly cubic perovskite structure. The introduction of Co3O4 promotes remarkably the electronic conductivity of the Y0.07Sr0.93Ti0.6Fe0.4-xO3-δ/x/3Co3O4 composites due to the formation of n-type CoO and p-type Co2O3. A limiting current oxygen sensor based on (Y0.07Sr0.93Ti0.6Fe0.4-xO3-δ)/x/3Co3O4 as a dense diffusion barrier shows excellent sensing performance. The recovery time is less than the response time, indicating that Co2O3 promotes the gas desorption reaction which results in a shorter recovery time. The obtained results demonstrate a direct relationship between limiting current (IL) and oxygen content.

13.
Virology ; 546: 79-87, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-32452419

RESUMO

In a previous study, we have shown that highly-pathogenic PRRSV (HP-PRRSV) nonstructural protein 4 (nsp4) antagonizes type I IFN expression induced by poly(I:C). Here, we demonstrated that the mutation of Aspartic acid 185 (Asp185) impaired the ability of nsp4 to inhibit IFN-I production induced by poly(I:C). Subsequently, we verified that all the mutants at the residue 185, regardless of amino acid size (including Cys and Ser) and charge (including Glu and Lys), impaired nsp4 catalytic activity. However, when Asp185 in nsp4 was replaced by a similar structure amino acid Asparagine 185 (Asn185), nsp4 stayed but with a decreased protease activity. Importantly, the recombinant virus with Asn185 mutation in HP-PRRSV-nsp4 exhibited slower replication rate and higher ability to induce IFN-I expression compared with wild-type (wt) HP-PRRSV.


Assuntos
Ácido Aspártico/metabolismo , Interferon beta/metabolismo , Síndrome Respiratória e Reprodutiva Suína/metabolismo , Síndrome Respiratória e Reprodutiva Suína/virologia , Vírus da Síndrome Respiratória e Reprodutiva Suína/metabolismo , Proteínas não Estruturais Virais/química , Proteínas não Estruturais Virais/metabolismo , Animais , Interações Hospedeiro-Patógeno , Interferon beta/genética , Síndrome Respiratória e Reprodutiva Suína/genética , Vírus da Síndrome Respiratória e Reprodutiva Suína/química , Vírus da Síndrome Respiratória e Reprodutiva Suína/genética , Vírus da Síndrome Respiratória e Reprodutiva Suína/patogenicidade , Suínos , Proteínas não Estruturais Virais/genética , Virulência
14.
J Nutr ; 150(2): 294-302, 2020 02 01.
Artigo em Inglês | MEDLINE | ID: mdl-31618431

RESUMO

BACKGROUND: Ectopic fat accumulation in skeletal muscle results in dysfunction and atrophy, but the underlying molecular mechanisms remain unclear. OBJECTIVE: The aim of this study was to investigate the effects of a high-fat diet (HFD) in modulating the structure and energy metabolism of skeletal muscle and the underlying mechanisms in mice. METHODS: Four-week-old male C57BL/6 J mice (n = 30) were allowed 1 wk for acclimatization. After 6 mice with low body weight were removed from the study, the remaining 24 mice were fed with a normal-fat diet (NFD; 10% energy from fat, n = 12) or an HFD (60% energy from fat, n = 12) for 24 wk. At the end of the experiment, serum glucose and lipid concentrations were measured, and skeletal muscle was collected for atrophy analysis, inflammation measurements, and phosphoproteomic analysis. RESULTS: Compared with the NFD, the HFD increased (P < 0.05) body weight (35.8%), serum glucose (64.5%), and lipid (27.3%) concentrations, along with elevated (P < 0.05) expressions of the atrophy-related proteins muscle ring finger 1 (MURF1; 27.6%) and muscle atrophy F-box (MAFBX; 44.5%) in skeletal muscle. Phosphoproteomic analysis illustrated 64 proteins with differential degrees of phosphorylation between the HFD and NFD groups. These proteins were mainly involved in modulating cytoskeleton [adenylyl cyclase-associated protein 2 (CAP2) and actin-α skeletal muscle (ACTA1)], inflammation [NF-κB-activating protein (NKAP) and serine/threonine-protein kinase RIO3 (RIOK3)], glucose metabolism [Cdc42-interacting protein 4 (TRIP10); protein kinase C, and casein kinase II substrate protein 3 (PACSIN3)], and protein degradation [heat shock protein 90 kDa (HSP90AA1)]. The HFD-induced inhibitions of the insulin signaling pathway and activations of inflammation in skeletal muscle were verified by Western blot analysis. CONCLUSIONS: Quantitative phosphoproteomic analysis in C57BL/6 J mice fed an NFD or HFD for 24 wk revealed that the phosphorylation of inflammatory proteins and proteins associated with glucose metabolism at specific serine residues may play critical roles in the regulation of skeletal muscle atrophy induced by an HFD. This work provides information regarding underlying molecular mechanisms for inflammation-induced dysfunction and atrophy in skeletal muscle.


Assuntos
Dieta Hiperlipídica , Inflamação/metabolismo , Músculo Esquelético/metabolismo , Atrofia Muscular/metabolismo , Fosfoproteínas/metabolismo , Proteômica , Animais , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Obesidade/metabolismo , Fosforilação , Proteólise , Transdução de Sinais
15.
ISA Trans ; 99: 231-239, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31733891

RESUMO

In quasi-static or static environments, generating secret key from wireless channels is bad randomness and vulnerable to active attacks. To improve randomness of the generated secret key, singular value decomposition (SVD) techniques are used to construct equivalent wireless channels in multiple-input multiple-output (MIMO) systems. SVD techniques and private pilot are used to detect active attacks by authenticating sender based on wireless channels. To defend passive eavesdropping and active attacks, the concept of one time pad is used to encrypt wireless channels with private pilot. The proposed SKG scheme based on private pilot and SVD techniques can not only detect whether SKG suffers active attacks, but also defend man-in-the-middle attack, impersonation attack, signal injection attack, passive eavesdropping. What is more, the SKG scheme can obtain higher SKG rate and randomness than the approach via random beam-forming. Theoretical analysis and simulation results prove the above results again.

16.
Sci Rep ; 9(1): 19207, 2019 12 16.
Artigo em Inglês | MEDLINE | ID: mdl-31844138

RESUMO

Correlative light and electron microscopy (CLEM) combines the strengths of both light and electron imaging modalities and enables linking of biological spatiotemporal information from live-cell fluorescence light microscopy (fLM) to high-resolution cellular ultra-structures from cryo-electron microscopy and tomography (cryoEM/ET). This has been previously achieved by using fLM signals to localize the regions of interest under cryogenic conditions. The correlation process, however, is often tedious and time-consuming with low throughput and limited accuracy, because multiple correlation steps at different length scales are largely carried out manually. Here, we present an experimental workflow, AutoCLEM, which overcomes the existing limitations and improves the performance and throughput of CLEM methods, and associated software. The AutoCLEM system encompasses a high-speed confocal live-cell imaging module to acquire an automated fLM grid atlas that is linked to the cryoEM grid atlas, followed by cryofLM imaging after freezing. The fLM coordinates of the targeted areas are automatically converted to cryoEM/ET and refined using fluorescent fiducial beads. This AutoCLEM workflow significantly accelerates the correlation efficiency between live-cell fluorescence imaging and cryoEM/ET structural analysis, as demonstrated by visualizing human immunodeficiency virus type 1 (HIV-1) interacting with host cells.


Assuntos
Microscopia Crioeletrônica/métodos , Tomografia com Microscopia Eletrônica/métodos , Infecções por HIV/patologia , Infecções por HIV/virologia , Microscopia de Fluorescência/métodos , Células Cultivadas , Células HEK293 , HIV-1/patogenicidade , Células Endoteliais da Veia Umbilical Humana , Humanos , Software , Fluxo de Trabalho
17.
Aging (Albany NY) ; 11(22): 10664-10683, 2019 11 24.
Artigo em Inglês | MEDLINE | ID: mdl-31761787

RESUMO

In this study, we obtained the RNA expression data of murine skin tissues of control, and UVB irradiated groups. After the re-annotation of lncRNAs, a gene expression similarity analysis was done by WGCNA. The target mRNA prediction of lncRNAs, miRNAs, and ceRNA regulatory networks were constructed by five lncRNAs, 14 miRNAs and 54 mRNAs, respectively. Based on the ceRNA network of UVB-induced skin lesions, it was evident that the dysregulation of Meg3 has critical effects on the UVB-induced inflammatory lesion of murine skin tissues. The overexpression of Meg3 after UVB irradiation was observed in primary murine skin fibroblasts, and the up-regulated Meg3 expression was related to the activation of the inflammatory cytokines. These functional experiments demonstrated that the RNA silencing of Meg3 in murine skin fibroblasts could suppress the expression of the cytokines (in vitro) and UVB-induced skin lesions (in vivo). Moreover, the Meg3 functioned as a competing endogenous RNA (ceRNA) that acted as a sponge for miR-93-5p and thereby modulated the expression of Epiregulin (Ereg). Our results proved that Meg3 was involved in UVB-induced skin inflammation and that the ceRNA networks, which includes miR-93-5p and Ereg, could prove to be a potential therapeutic target for UVB-induced skin damage.


Assuntos
Epirregulina/metabolismo , Inflamação/metabolismo , MicroRNAs/metabolismo , RNA Longo não Codificante/metabolismo , Transdução de Sinais/efeitos da radiação , Pele/efeitos da radiação , Raios Ultravioleta/efeitos adversos , Animais , Epirregulina/efeitos da radiação , Fibroblastos/metabolismo , Fibroblastos/efeitos da radiação , Regulação da Expressão Gênica/efeitos da radiação , Redes Reguladoras de Genes , Inflamação/etiologia , Camundongos , MicroRNAs/efeitos da radiação , RNA Longo não Codificante/efeitos da radiação , Transdução de Sinais/fisiologia , Pele/metabolismo
18.
Int Immunopharmacol ; 75: 105817, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31446161

RESUMO

Artesunate (ART), a derivative of artemisinin, is a medication to treat malaria. Beyond that, the anti-inflammatory and immunoregulatory activities of ART have been identified in autoimmune diseases. However, whether ART functions in psoriasis-like dermatitis induced by imiquimod (IMQ, a TLR7/8 agonist) is currently unkown. There, we found that the cumulative score, epidermal thickening and expression of Ki-67 of ART-treated BALB/c mice were significantly lower than those in the IMQ psoriatic model group. In addition, ART treatment ameliorated mice from systemic inflammation. Mechanistically, ART reduced γδ T cells in draining lymph nodes, which might be benefit the improvement of dermatitis. These findings suggested that ART could be a promising drug of psoriasis in clinic.


Assuntos
Anti-Inflamatórios/uso terapêutico , Artesunato/uso terapêutico , Dermatite/tratamento farmacológico , Psoríase/tratamento farmacológico , Animais , Anti-Inflamatórios/farmacologia , Artesunato/farmacologia , Dermatite/imunologia , Dermatite/patologia , Imiquimode , Linfócitos Intraepiteliais/efeitos dos fármacos , Linfócitos Intraepiteliais/imunologia , Linfonodos/efeitos dos fármacos , Linfonodos/imunologia , Masculino , Camundongos Endogâmicos BALB C , Psoríase/imunologia , Psoríase/patologia , Pele/efeitos dos fármacos , Pele/imunologia , Pele/patologia , Células Th17/efeitos dos fármacos , Células Th17/imunologia
19.
BMJ Open ; 9(6): e025773, 2019 06 16.
Artigo em Inglês | MEDLINE | ID: mdl-31209084

RESUMO

OBJECTIVES: Haemorrhagic fever with renal syndrome (HFRS) is a serious threat to public health in China, accounting for almost 90% cases reported globally. Infectious disease prediction may help in disease prevention despite some uncontrollable influence factors. This study conducted a comparison between a hybrid model and two single models in forecasting the monthly incidence of HFRS in China. DESIGN: Time-series study. SETTING: The People's Republic of China. METHODS: Autoregressive integrated moving average (ARIMA) model, generalised regression neural network (GRNN) model and hybrid ARIMA-GRNN model were constructed by R V.3.4.3 software. The monthly reported incidence of HFRS from January 2011 to May 2018 were adopted to evaluate models' performance. Root mean square error (RMSE), mean absolute error (MAE) and mean absolute percentage error (MAPE) were adopted to evaluate these models' effectiveness. Spatial stratified heterogeneity of the time series was tested by month and another GRNN model was built with a new series. RESULTS: The monthly incidence of HFRS in the past several years showed a slight downtrend and obvious seasonal variation. A total of four plausible ARIMA models were built and ARIMA(2,1,1) (2,1,1)12 model was selected as the optimal model in HFRS fitting. The smooth factors of the basic GRNN model and the hybrid model were 0.027 and 0.043, respectively. The single ARIMA model was the best in fitting part (MAPE=9.1154, MAE=89.0302, RMSE=138.8356) while the hybrid model was the best in prediction (MAPE=17.8335, MAE=152.3013, RMSE=196.4682). GRNN model was revised by building model with new series and the forecasting performance of revised model (MAPE=17.6095, MAE=163.8000, RMSE=169.4751) was better than original GRNN model (MAPE=19.2029, MAE=177.0356, RMSE=202.1684). CONCLUSIONS: The hybrid ARIMA-GRNN model was better than single ARIMA and basic GRNN model in forecasting monthly incidence of HFRS in China. It could be considered as a decision-making tool in HFRS prevention and control.


Assuntos
Febre Hemorrágica com Síndrome Renal/epidemiologia , Modelos Estatísticos , Redes Neurais de Computação , China/epidemiologia , Previsões , Humanos , Incidência , Estações do Ano , Software
20.
World J Clin Cases ; 7(7): 903-907, 2019 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-31024963

RESUMO

BACKGROUND: Congenital extrahepatic portosystemic shunt, also known as Abernethy deformation, is a rare malformation caused by dysplasia in the portal vein system. There are few reports of liver transplantation as a treatment for Abernethy deformation, and our report is the first case in China. This is the second reported case with congenital extrahepatic portosystemic shunt combined with focal nodular hyperplasia and hepatopulmonary treated with liver transplantation. CASE SUMMARY: The patient was a 14-year-old girl, diagnosed preoperatively as type Ib Abernethy deformation, intrahepatic multiple space-occupying lesion, and hepatopulmonary syndrome. The patient recovered well after undergoing classic orthotopic liver transplantation. Liver function, pulmonary function, and portal vein computed tomography angiography imaging were reexamined 20 mo postoperatively, and no abnormality was observed. CONCLUSION: Liver transplantation is an effective treatment for type I Abernethy deformation combined with focal nodular hyperplasia and hepatopulmonary syndrome.

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