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1.
Int J Med Sci ; 17(10): 1385-1392, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32624695

RESUMO

Dry Eye Disease (DED) is a common ocular condition that needs prompt diagnosis and careful treatment interventions. If left untreated, it can lead to numerous sight-threatening complications, including ulceration of the cornea, blepharitis, alterations of the tear film, conjunctivitis, and in severe cases, may lead to scarring, thinning, and even perforation of the cornea. Intense pulsed light (IPL) is a non-laser high-intensity light source that has shown to play a valuable role in dry eye disease. Recent evidence from various research works has shown that IPL modifies the mechanism of meibomian gland dysfunction (MGD), which helps to relieve the symptoms of DED. In this review, we demonstrated the mechanism of action of IPL, including its benefits on DED. The emerging evidence shows that the role of IPL in DED is novel and therapeutic. These results direct us to conclude that IPL is a potentially beneficial tool and essential future therapy for dry eye disease. Advances in the treatment of DED will lead to a better quality of life. However, tools to recognize potentially severe side effects of DED earlier in order to treat or prevent them must be developed.

2.
Artigo em Inglês | MEDLINE | ID: mdl-32500492

RESUMO

Intracellular phosphorus (P) accumulation can improve microalgal growth and lipid synthesis. However, large excess of P causes cell poisoning. This study utilized a P-fed-batch strategy to investigate its potential to improve the utilization of the excessive P, while avoiding toxic side effects. This strategy contributed to a more complete utilization of the intracellularly stored P, which enhanced the microalgae biomass by 10-15% by upregulating the brassinosteroid growth hormone gene at a P-fed-batch frequency of 2-8. Furthermore, the lipid content increased by 4-16% via upregulation of lipid synthesis-related genes. As a result, the P-fed-batch strategy significantly increased the lipid production by 13-19%. The content of saturated fatty acid increased by ~ 100%, implying improved combustibility and oxidative stability. This is the first study of this P-fed-batch strategy and provides a new concept for the complete utilization of excessive P.

3.
J Agric Food Chem ; 68(21): 5900-5909, 2020 May 27.
Artigo em Inglês | MEDLINE | ID: mdl-32348140

RESUMO

In this study, the effects of white light-emitting diode (LED) and UV-C radiation (with the same intensity) on stilbene biosynthesis and phytochemicals accumulation of peanut sprouts were investigated. Results showed that white light radiation promoted the growth of peanut sprouts while UV-C radiation had the opposite effect. Contents of total phenolics, total flavonoids, and phytochemicals significantly increased in peanut sprouts treated by white light or UV-C radiation. Besides, light radiation significantly induced stilbene accumulation by upregulating the expression of genes and enzymes in stilbene biosynthesis-related pathway, and UV-C was more effective to promote stilbene accumulation. Compared with piceid and piceatannol, resveratrol showed the highest accumulation in peanut sprouts treated by light radiation. In summary, white light or UV-C radiation could be used as a method to promote stilbene biosynthesis and phytochemicals accumulation in peanut sprouts and UV-C was more effective.

4.
Scand J Gastroenterol ; 55(4): 442-448, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32285715

RESUMO

Aims: Granuloma, mainly composed of macrophages, is a histological feature of Crohn's disease (CD). However, its significance in CD has not been investigated adequately. Our study aims to address this issue by comparing the clinical manifestations and monocyte/macrophage subtypes between granulomatous and non-granulomatous CD.Materials and methods: Demographics, symptoms, endoscopic manifestations, histopathological features, and Montreal classification of patients with and without granulomas were compared. Flow cytometry was used to determine the phagocytosis and subsets of monocytes. ELISA was used to measure the plasma levels of TNF-α, IL-6, IL-1ß, IL-10, CCL22, and TGF-ß1. Immunohistochemistry was performed to quantify the expression of CD68, CD163 and iNOS.Results: Of the222 CD patients enrolled, granulomas were detected in 90. Compared with non-granulomatous CD patients, those with granulomas had younger age, increased rates of diarrhea and perianal complications, along with higher endoscopic score. Intestinal stenosis and crypt abscess were more frequently observed in granulomatous CD patients. A defective phagocytosis of monocytes was observed in granulomatous CD patients. Meanwhile, higher percentages of intermediate and non-classic monocytes, with a lower percentage of classic monocyte were found in them. Besides, they had higher levels of TGF-ß1 and IL-10, a lower level of TNF-α, an increased ratio of CD163+/CD68+cells, and a decreased ratio of iNOS+/CD68+ cells.Conclusions: Granulomatous CD patients exhibited different manifestations compared with their non-granulomatous counterparts. More aggressive therapy may be needed in granulomatous CD patients. Furthermore, the heterogeneity of monocyte/macrophage subsets and altered plasma cytokine may underlie the difference between those two groups.

5.
J Mol Neurosci ; 70(7): 1164, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32342254

RESUMO

The original version of this article unfortunately contained mistakes in the Author Group section. Authors Dandan Zhou and Lei Ji were assigned to incorrect institutions.

6.
J Hazard Mater ; 394: 122574, 2020 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-32278124

RESUMO

ß-Lactam antibiotics are the most commonly used antibiotics, and are difficult to remove by conventional biological treatments because of their persistent and toxic nature. The addition of co-substrates has been successfully employed to improve the removal of refractory pollutants. So, we hypothesized that the co-substrate strategy would increase antibiotic degradation and benefit microbial survival. In this work, we reported that co-substrate (acetate) addition up-regulated key degrading enzymes and resistance related genes in a model bacteria strain (L. aquatilis) when being treated with 0.055 mM amoxicillin (AMO). ß-Lactamase, amidases, transaminase, and amide C-N hydrolase showed increased activation. As a result, AMO removal reached ∼95 %, a ∼60 % increase over the control. Furthermore, the addition of acetate drove the down-stream TCA cycle, which accelerated the detoxification of the intermediates and reduced the microbial inhibition by the antibiotic products to as low as ∼15 %. Besides, the expression levels of genes encoding the efflux pump, penicillin binding proteins, and ß-Lactamase were up-regulated, and the inhibition of peptidoglycan biosynthesis was down-regulated. The cell density was enhanced by ∼170 % and showed improved DNA replication. In conclusion, the addition of the co-substrate accelerated AMO degradation and detoxification by up-regulating degrading enzymes and promoting cell resistance.

7.
J Colloid Interface Sci ; 569: 195-203, 2020 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-32113016

RESUMO

Green microalgae are promising and sustainable bioenergy and biomass feedstocks, that specifically utilize blue and red light for photosynthesis. Using carbon dots (CDs) to optimize photoluminescence is an attractive strategy for enhancing microalgal bioresource production; however, CD synthesis traditionally requires harsh conditions and laborious procedures. Little research has focused on developing CDs with tunable emission that precisely satisfy photosynthetic requirements. In this work, we directionally prepared non-toxic CDs using a simple method, which could adsorb light at spectra 500-600 nm and emit red light at 580-700 nm. CDs significantly promoted microalgae (Chlorella) growth by ~15%. Meanwhile, potential intracellular bioresources, pigments, carbohydrates, proteins, and lipids were generally enhanced. CDs, combined with an extracellular polymeric substance of microalgal cells, served as numerous micro-bulbs for Chlorella irradiation to sustainably provide optimized light. In this context, photosystems I and II were both stimulated. As such, we prepared CDs with tunable emission, which could significantly enhance microalgae and bioresource production.

9.
ACS Appl Mater Interfaces ; 12(10): 12143-12154, 2020 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-32078286

RESUMO

The development of valuable theranostic agents for overcoming the blood-brain barrier (BBB) to achieve efficient imaging-guided glioma-targeting delivery of therapeutics remains a great challenge for personalized glioma therapy. We herein developed a novel functional star-shaped polyprodrug amphiphile (denoted as CPP-2) via a combination of successive reversible addition-fragmentation chain transfer (RAFT) polymerization and click functionalization. In a diluted solution, the star amphiphile existed as structurally stable unimolecular micelles, containing hydrophobic cores conjugated with reduction-responsive camptothecin prodrugs Camptothecin (CPT) prodrug monomer (CPTM) and a tertiary amine monomer (2-(diethylamine) ethyl methacrylate, DEA) and hydrophilic oligo-(ethylene glycol) monomethyl ether methacrylat (OEGMA) outer coronas covalently decorated with dual-targeting moieties Angiopep2 (ANG) and small magnetic resonance imaging (MRI) contrast agents DOTA-Gd. In vitro and in vivo data in this study demonstrated that the ANG-modified micelles were capable of efficiently penetrating the BBB and delivering loaded cargoes such as CPT and Gd3+ contrast agents to glioma cells, leading to a considerably enhanced t1 relaxivity as well as antiglioma efficacy. Simultaneously, the targeted antiglioma efficacy and noninvasive MR imaging for a visualized therapy were realized. These collective findings augured well for the star polyprodrug amphiphiles to be utilized as a novel theranostic platform for clinical application in glioma therapy.

10.
Bioresour Technol ; 304: 123009, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32087545

RESUMO

In this study, a new intimately coupling technology of advanced oxidation and biodegradation was proposed, called simultaneous combination of ozonation and biodegradation (SCOB), which uses ozonation in place of traditional photocatalysis. SCOB was evaluated for its ability to degrade and detoxify tetracycline hydrochloride (TCH). Biodegradation alone only resulted in negligible TCH removal, while ozone alone caused less effective performance, with TCH degradation rate constants of 29-171% lower than those of SCOB. The optimal ozone dose was 2.0 mg-O3/(L·h), and it contributed to remove 97% of the TCH within 2 h under SCOB operation. The SCOB effluent was not toxic to S. aureus after 8 h of exposure. During six SCOB operation cycles, the biomass in the biofilm remained stable, and cell structure was relatively intact. SCOB significantly improved TCH degradation and reduced toxicity of the effluent.


Assuntos
Ozônio , Poluentes Químicos da Água , Antibacterianos , Biodegradação Ambiental , Staphylococcus aureus , Tetraciclina
11.
Brain Cogn ; 140: 105533, 2020 04.
Artigo em Inglês | MEDLINE | ID: mdl-32018217

RESUMO

We used voxel-based morphometry and resting-state functional magnetic resonance imaging (rs-fMRI) to investigate whether the regulatory mode orientation of assessment (the tendency of each individual to self-regulate by critically evaluating alternatives) interacts with neural systems underlying risk-taking. Across a sample of 112 participants, propensity for risk-taking (measured using the Wheel of Fortune task) was negatively correlated with assessment orientation, such that a greater tendency to critically evaluate alternatives was associated with a lower tendency for risk-taking. VBM revealed a negative correlation between assessment orientation and right inferior parietal lobe (RIPL) gray matter volume. Resting-state functional connectivity (rs-FC) between this same RIPL region and the left inferior frontal gyrus (LIFG) was positively correlated with assessment orientation in an independent sample of 41 participants. Most importantly, based on the rs-FC results, a mediation analysis indicated that assessment orientation played a completely mediating role in the relation between the functional connectivity of RIPL-LIFG and risk-taking. These results suggest that assessment orientation may affect risk-taking via the RIPL and its connectivity with LIFG. On the whole, the present study yields the insights into how the assessment dimension of regulatory mode affects risk-taking, and provides a novel account of the neural substrate of this relationship.

13.
PLoS One ; 15(1): e0227408, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31940393

RESUMO

Pepsin plays an important role in laryngopharyngeal reflux (LPR), a risk factor for the development of hypopharyngeal squamous cell carcinomas (HPSCC). However, the role of pepsin in HPSCC is not clear. We show by immunohistochemistry that pepsin positivity occurs in a significant proportion of human primary HPSCC specimens, and in many cases matched adjacent uninvolved epithelia are negative for pepsin. Pepsin positivity is associated with nodal involvement, suggesting that pepsin may have a role in metastasis. Treatment of FaDu cancer cells with pepsin increased cell proliferation, possibly by inducing G1/S transition. We also observed significant changes in expression of genes involved in NF-kappaB, TRAIL and Notch signaling. Our data suggest that pepsin plays an important role in HPSCC and that targeting pepsin could have potential therapeutic benefits.


Assuntos
Carcinoma de Células Escamosas/metabolismo , Fase G1 , Regulação Neoplásica da Expressão Gênica , Neoplasias Laríngeas/metabolismo , Proteínas de Neoplasias/metabolismo , Pepsina A/metabolismo , Neoplasias Faríngeas/metabolismo , Fase S , Transdução de Sinais , Idoso , Carcinoma de Células Escamosas/patologia , Linhagem Celular Tumoral , Feminino , Humanos , Neoplasias Laríngeas/patologia , Masculino , Pessoa de Meia-Idade , Metástase Neoplásica , Pepsina A/farmacologia , Neoplasias Faríngeas/patologia
14.
Cancer Lett ; 474: 23-35, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-31931029

RESUMO

Despite the success of epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs) in the treatment of non-small cell lung cancer (NSCLC) harboring EGFR-activating mutations, intrinsic or acquired resistance remains the major obstacle to long-term disease remission. Defective autophagy has been reported as an EGFR-TKI resistance mechanism. However, how EGFR regulate autophagic flux are still not fully understood. Here we found that EGFR-stimulated phosphorylation of SQSTM1 at tyrosine 433 induces dimerization of its UBA domain, which disturbs the sequestration function of SQSTM1 and causes autophagic flux blocking. SAH-EJ2, a staple optimized EGFR-derived peptide, showed enhanced in vitro and in vivo antitumor activity against NSCLC than the prototype regardless of EGFR mutation status. Mechanistically, SAH-EJ2 disrupts the EGFR-SQSTM1 interaction and protects against EGFR-induced SQSTM1 phosphorylation, which hinders the dimerization of the SQSTM1 UBA domains and restores SQSTM1 cargo function. Moreover, SAH-EJ2 suppresses EGFR activity by blocking its dimerization and reducing its protein stability, which reciprocally activates the core autophagy machinery. Our observations reveal that disturbing the EGFR-SQSTM1 interaction by SAH-EJ2 confers a potential strategy in the treatment of NSCLC through suppressing EGFR signalling and activating autophagy simultaneously.

15.
Q J Exp Psychol (Hove) ; 73(8): 1150-1161, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31910719

RESUMO

Spatial-numerical and spatial-positional associations have been well documented in the domains of numerical cognition and working memory, respectively. However, such associations are typically calculated by directly comparing (e.g., subtracting) left- versus right-hand responses; it remains an open question whether such associations reside in each hand individually, or are exclusively localised in one of the two hands. We conducted six experiments to investigate the hand-lateralization of both spatial-numerical and spatial-positional associations. All experiments revealed that the spatial associations stemmed from left-hand responses, irrespective of the handedness of the subjects, but with the exception of the magnitude comparison task (Experiments 5 and 6). We propose that the hemispheric lateralization of the tasks in combination with the task-relevance of spatial associations can explain this pattern. More generally, we suggest that the contributions of left and right hands require more attention in spatial-numerical and spatial-positional research.

16.
J Agric Food Chem ; 68(6): 1579-1587, 2020 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-31760750

RESUMO

Organic anion transporter 3 (OAT3) plays a critical role in the renal excretion of many xenobiotics. Because steviol acyl glucuronide (SVAG), an OAT3 substrate, is the major circulating metabolite after oral ingestion of steviol glycosides and is excreted into the urine, inhibition of OAT3 activity may alter pharmacokinetic profiles of SVAG. The present study showed that drugs such as probenecid and glimepiride displayed potent inhibition toward the OAT3-mediated SVAG transport, with IC50 values of 4.9 and 0.8 µM, respectively. No species differences were observed. Probenecid and glimepiride could significantly elevate plasma concentrations of SVAG after oral administration of rebaudioside A, with significant increases in plasma maximum (Cmax) and area under the plasma time-concentration curve values. The inhibitory effect on the OAT3-mediated SVAG transport exemplified a unique case between drugs and the metabolite of a food additive. Our data suggest that caution should be exercised when giving steviol glycoside products to human subjects with compromised renal function.


Assuntos
Diterpenos de Caurano/metabolismo , Glucosídeos/metabolismo , Transportadores de Ânions Orgânicos Sódio-Independentes/metabolismo , Probenecid/metabolismo , Compostos de Sulfonilureia/metabolismo , Animais , Transporte Biológico , Diterpenos de Caurano/química , Glucosídeos/química , Células HEK293 , Humanos , Rim/efeitos dos fármacos , Rim/metabolismo , Masculino , Transportadores de Ânions Orgânicos Sódio-Independentes/genética , Probenecid/administração & dosagem , Probenecid/química , Ratos , Ratos Sprague-Dawley , Compostos de Sulfonilureia/administração & dosagem , Compostos de Sulfonilureia/química
17.
Environ Sci Pollut Res Int ; 27(15): 17516-17529, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-31236868

RESUMO

The photocatalytic technology provides a promising and effective strategy for the transformation and degradation of contaminants. Herein, we accurately fabricated a novel ternary photocatalyst, namely, metal silver (Ag) and carbon dots (CDots) co-doped BiOI nanocomposite (Ag/CDots/BiOI) via the reduction method with ionic liquids 1-butyl-3-methylimidazolium iodine ([Bmim]I) at room temperature. The morphologies and microstructures showed the Ag and CDots were uniformly loaded on the surface of BiOI, forming a ternary system. The characterization results implied that an intense interaction was formed between Ag and CDots on the BiOI, which could achieve the broad spectrum utilization of visible light and boosted the photocatalytic performances. The 0.9-Ag/2-CDots/BiOI (0.9 wt% of Ag, 2 wt% of CDots) presented the highest photocatalytic activity with ~ 100% in 4-Chlorophenol, 68.8% in mineralization, and 87.4% in dechlorination in 6 h under visible light illumination. The enhanced photocatalytic activity could be ascribed to the surface plasmon resonance effect of Ag, the up-converted photoluminescence (PL) properties of CDots, and the electron transfer properties of both Ag and CDots. Moreover, a possible photocatalytic reaction mechanism was discussed in detail by band structure analysis and radical scavenger quenching experiments. This study provides a promising approach for promoting the utilization efficiency for solar energy and sustainable environmental remediation.

18.
Food Chem ; 309: 125726, 2020 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-31704069

RESUMO

In our present study, we aimed to explore the effects of hot air and UV-C on anthocyanins and the interaction among anthocyanin, sucrose and organic acids in peaches during postharvest storage. Peaches were treated with hot air or UV-C and stored at 1 °C for 35 days. The results showed that both treatments significantly enhanced the accumulation of anthocyanins and suppressed the degradation of sucrose, citric and malic acids. An in vitro test verified that sucrose, citric and malic acid penetrated the tissue and then induced the biosynthesis of anthocyanins by up regulating anthocyanin-related enzymes. In addition, hot air and UV-C directly enhanced the activities and gene expression of related enzymes to promote the accumulation of anthocyanins. PAL, ANS and UFGT played crucial roles in the biosynthesis of anthocyanins in peach fruit after harvest, and these three enzymes can be stimulated by HA, UV-C, sucrose, citric and malic acid.


Assuntos
Antocianinas/metabolismo , Prunus persica/química , Raios Ultravioleta , Antocianinas/química , Armazenamento de Alimentos , Frutas/química , Frutas/metabolismo , Regulação da Expressão Gênica de Plantas/efeitos da radiação , Temperatura Alta , Malatos/química , Malatos/metabolismo , Prunus persica/metabolismo , Prunus persica/efeitos da radiação , Açúcares/química , Açúcares/metabolismo
19.
Acta Trop ; 201: 105219, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31614120

RESUMO

As the only specialized institution for research and control of parasitic diseases at the national level in China for almost 70 years, the National Institute of Parasitic Diseases (NIPD) at the Chinese Center for Disease Control and Prevention (China CDC) has been instrumental in supporting the remarkable progress from high prevalence to transmission interruption or low endemicity of several diseases, lymphatic filariasis, malaria and schistosomiasis in particular. This has taken place through technical guidance, emergency response and scientific research as well as providing technical service, education, training, health promotion and international cooperation. With China's increasing involvement in international cooperation and the increased risk for (re)emerging tropical diseases in mind, the Chinese Government designated in 2017 a new Chinese Center for Tropical Disease Research to NIPD. Responding to the expanded responsibilities, the institute is scaling up its activities in several ways: from parasitic diseases to the wider area of tropical diseases; from disease control to disease elimination; from biological research to policy evidences accumulation; and from public health to global health. Based on this new vision and China's previous accomplishments in the areas mentioned, the institute is in a position to move forward with respect to global health and equitable development according to the central principles of the United Nations' Sustainable Development Goals.


Assuntos
Centers for Disease Control and Prevention, U.S./organização & administração , Doenças Transmissíveis/epidemiologia , Saúde Global/normas , Cooperação Internacional , Doenças Parasitárias/epidemiologia , Saúde Pública/normas , Medicina Tropical/normas , Academias e Institutos , Animais , China/epidemiologia , Humanos , Objetivos Organizacionais , Estados Unidos
20.
Chemosphere ; 245: 125612, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31864948

RESUMO

Humic substances are the dominant dissolved organic matter fraction in the aqueous phase of environmental media. They would inevitably react with chemicals released into the environment. The influence of dissolved humic acid (DHA) on the dissolution and dispersion of copper oxide nanoparticles (CuO NPs, 50 nm, 49.57 mg L-1) was therefore investigated in the present study. In addition to dispersing CuO NPs and reducing the size of the aggregates, the amount of released Cu from CuO NPs was found to increase over time with increasing concentrations of DHA, 96% of which was present as organic complexes after 72 h. At DHA concentrations exceeding 16.09 mg C L-1, the complexation coefficients of DHA with Cu and the adsorptivity of CuO NPs to DHA were both reduced due to increased homo-conjugation of DHA as promoted by negative charge-assisted H-bond. Although the adsorption capacity of DHA kept increasing up to 57.07 mg C L-1, the hydrodynamic diameter and ζ-potential were similar and the percentages of total released Cu continued to increase linearly to 4.92% at higher levels of DHA (30.13-57.07 mg C L-1). Thereupon, DHA promoted the dissolution of CuO NPs in a concentration-dependent fashion. The driving force was complexation of Cu by DHA, rather than the balancing between the exposed and the covered surface area of the CuO NPs due to DHA adsorption. Our findings facilitate understanding the underlying mechanisms on how DHA impacts the CuO NPs environmental behavior (or fate) as well as on their kinetics.


Assuntos
Cobre/química , Substâncias Húmicas , Nanopartículas Metálicas/química , Adsorção , Difusão , Cinética , Solubilidade , Propriedades de Superfície
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