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Oncotarget ; 8(57): 97476-97489, 2017 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-29228625


Chemotherapeutic insensitivity remains one of the major obstacles in clinical treatment of lung squamous cell carcinoma (LSCC). Recently, increasing evidence has suggested that long non-coding RNAs (lncRNAs) promote tumorigenesis in many cancer types. However, the potential biological roles and regulatory mechanisms of lncRNAs in response to cisplatin treatment are poorly understood. Here, we found that lncRNA SFTA1P (surfactant associated 1, pseudogene), highly expressed in lung, was down-regulated in LSCC tissues and could be induced upon cisplatin treatment in LSCC cells. Elevated SFTA1P induced apoptosis and enhanced the sensitivity to cisplatin of LSCC cells. We further identified that hnRNP-U (heterogeneous nuclear ribonucleoprotein U) was down-regulated in LSCCs and positively correlated with patients' poor prognosis as well as SFTA1P. Mechanistic studies revealed that SFTA1P could up-regulate hnRNP-U expression. In addition, we identified that hnRNP-U enhanced cisplatin-induced apoptosis through up-regulation of GADD45A, high expression of which was correlated with good prognosis in LSCC patients. Our findings demonstrated that SFTA1P might serve as a useful biomarker for LSCC diagnosis and a predictor for cisplatin chemotherapy response in patients with LSCC.

Int J Chron Obstruct Pulmon Dis ; 12: 1247-1254, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28490868


COPD is a chronic airway inflammatory disease characterized mainly by neutrophil airway infiltrations. Interleukin (IL)-1ß and IL-17 are the key mediators of neutrophilic airway inflammation in COPD. This study was undertaken to evaluate the serum IL-1ß and IL-17 levels and associations between these two key mediators with clinical parameters in COPD patients. Serum samples were collected from 60 COPD subjects during the acute exacerbation of COPD, 60 subjects with stable COPD and 40 healthy control subjects. Commercial enzyme-linked immunosorbent assay kits were used to measure the serum IL-1ß and IL-17 concentrations. The association between serum IL-1ß and IL-17 with FEV1% predicted, C-reactive protein, neutrophil percentage and smoking status (pack-years) was assessed in the COPD patients. We found that serum IL-1ß and IL-17 levels in acute exacerbation of COPD subjects were significantly higher than that in stable COPD or control subjects and were positively correlated to serum C-reactive protein levels, neutrophil % and smoking status (pack-years) but negatively correlated with FEV1% predicted in COPD patients. More importantly, serum IL-1ß levels were markedly positively associated with serum IL-17 levels in patients with COPD (P=0.741, P<0.001). In conclusion, elevated serum IL-1ß and IL-17 levels may be used as a biomarker for indicating persistent neutrophilic airway inflammation and potential ongoing exacerbation of COPD.

Mediadores da Inflamação/sangue , Interleucina-17/sangue , Interleucina-1beta/sangue , Pulmão/fisiopatologia , Doença Pulmonar Obstrutiva Crônica/sangue , Idoso , Biomarcadores/sangue , Proteína C-Reativa/análise , Estudos de Casos e Controles , Estudos Transversais , Progressão da Doença , Ensaio de Imunoadsorção Enzimática , Feminino , Volume Expiratório Forçado , Humanos , Masculino , Pessoa de Meia-Idade , Neutrófilos/imunologia , Valor Preditivo dos Testes , Doença Pulmonar Obstrutiva Crônica/diagnóstico , Doença Pulmonar Obstrutiva Crônica/imunologia , Doença Pulmonar Obstrutiva Crônica/fisiopatologia , Fumar/efeitos adversos , Regulação para Cima
Talanta ; 83(5): 1716-20, 2011 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-21238774


CdTe quantum dots (QDs) were used as a highly selective probe for the detection of prion protein. Orange-emitting precipitates appeared within 30s of the addition of recombination prion protein (rPrP) to a solution of green-emitting CdTe QDs. This allowed colorimetric qualitative and semi-quantitative detection of rPrP. The decrease in fluorescence intensity of the supernatant could be used for quantitative detection of rPrP. The fluorescence intensity of the supernatant was inversely proportional to the rPrP concentration from 8 to 200 nmol L(-1) (R(2)=0.9897). Transmission electron microscopy results showed that fibrils existed in the precipitates and these were partly transformed to amyloid plaques after the addition of rPrP.

Compostos de Cádmio/química , Príons/análise , Pontos Quânticos , Espectrometria de Fluorescência/métodos , Telúrio/química , Colorimetria , Microscopia Eletrônica de Transmissão , Modelos Biológicos , Príons/química
Artigo em Inglês | MEDLINE | ID: mdl-20022294


Herein, we prepared water-soluble fluorescent carbon dots with diameter about 1.5 nm from cheap commercial lampblack. These fluorescent carbon nanoparticles are stable toward photobleaching and stable in water for more than half a year without fluorescence decrease. In order to improve its fluorescence properties, we passivated these nanoparticles with bisamino-terminated polyethylene glycol (PEG(1500 N)). Therefore, both fluorescence quantum yield and lifetime increased after this progress. In addition, the passivated carbon dots were more inert to solvent than the bare one and showed different responses to pH change.

Carbono/química , Fluorescência , Pontos Quânticos , Metais/química , Nanopartículas , Polietilenoglicóis/química
Artigo em Inglês | MEDLINE | ID: mdl-18715824


Herein, we reported the quenching effect of Ni(2+) on bovine serum albumin protected fluorescent gold nanoparticles (BSA-GNPs). The quenching mechanism was discussed and a static quenching mechanism was proposed. The number of binding sites (n), apparent stability constants (K) and corresponding thermodynamic parameters of BSA-GNPs-Ni(2+) complex were measured at different temperatures. Under optimum conditions, the fluorescence intensity of BSA-GNPs is linearly proportional to nickel concentration from 6.0x10(-8)mol/L to 8.0x10(-6)mol/L with a detection limit of 1.0x10(-8)mol/L. The result indicated that BSA-GNP was a potential Ni(2+) probe.

Ouro/química , Íons/farmacologia , Nanopartículas Metálicas/química , Níquel/farmacologia , Sítios de Ligação/efeitos dos fármacos , Fluorescência , Ouro/metabolismo , Íons/química , Íons/metabolismo , Microscopia Eletrônica de Transmissão , Níquel/química , Níquel/metabolismo , Soroalbumina Bovina/farmacologia , Espectrometria de Fluorescência
Zhong Nan Da Xue Xue Bao Yi Xue Ban ; 32(5): 868-72, 2007 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-18007086


OBJECTIVE: To explore the effect of ginsenoside Rh2 (G-Rh2) on the excretion of cytotoxin-effecting molecule of alveolar macrophages (AM) in patients with non-small cell lung cancer (NSCLC). METHODS: The concentration of tumor necrosis factor (TNF-alpha) and NO in the bronchoalveolar lavage fluid (BALF) and the cultured supernatants of AM in 35 patients with NSCLC were measured by ELISA and enzyme method,and levels of TNF-alpha and NO in the cultured supernatants of AM after being cultivated with IFN-alpha, G-Rh2, and IFN-alpha+G-Rh2 were measured by the same method. RESULTS: AM in all the non-small cell lung cancer patients produced TNF-alpha and NO. The activity of TNF-alpha and NO was lower in the BALF and in the cultured supernatants of AM of the tumor-bearing lungs than that of the non-tumor-bearing lungs. The concentrations of TNF-alpha and NO in the cultured supernatants of AM cultivated with G-Rh2 were higher than those in the control (P<0.05), but there were no significant differences between the G-Rh2 group and IFN-alpha group (P>0.05). The concentrations of TNF-alpha and NO in the cultured supernatants of AM cultivated with both G-Rh2 and IFNalpha were obviously higher than those stimulated with IFNalpha or G-Rh2 (P<0.01) alone. CONCLUSION: G-Rh2 can enhance the excretion of cytotoxin-effecting molecules of AM in patients with NSCLC. The changes are more distinctive when G-Rh2 and IFNalpha have coordinated action.

Carcinoma Pulmonar de Células não Pequenas/imunologia , Ginsenosídeos/farmacologia , Neoplasias Pulmonares/imunologia , Macrófagos Alveolares/imunologia , Adulto , Idoso , Líquido da Lavagem Broncoalveolar/química , Feminino , Humanos , Macrófagos Alveolares/efeitos dos fármacos , Macrófagos Alveolares/metabolismo , Masculino , Pessoa de Meia-Idade , Óxido Nítrico/metabolismo , Células Tumorais Cultivadas , Fator de Necrose Tumoral alfa/metabolismo