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1.
Fitoterapia ; : 104351, 2019 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-31476401

RESUMO

Three new sesquiterpenoids, (+)-(1R,2S,3R,4S,5R,6S,9R)-3,11,12-trihydroxypicrotoxane-2(15)-lactone (1), (-)-(1S,2R,3S,4R,5S,6R,9S,12R)-3,11,13-trihydroxypicrotoxane-2(15)-lactone (2), and (+)-(1R,5R,6S,8R,9R)-8,12-dihydroxy-copacamphan-3-en-2-one (3), together with five known compounds, were isolated from the n-butanol soluble fraction of a 95% EtOH extract of the stems of Dendrobium nobile. Their structures were determined by extensive spectroscopic analysis. Particularly, to solve difficult stereochemical problems, electronic circular dichroism calculations, NMR data calculations, and a single-crystal X-ray diffraction were performed. Interestingly, compounds 1 and 2 were picrotoxinin-type sesquiterpenoids with an unusual C15,2-lactone ring. All new sesquiterpenoids (1-3) showed a significant neuroprotective activity against H2O2-induced oxidative damage in PC12 cells. Notably, at 25 and 50 µM, compounds 1 and 2 showed the best protective effects, even better than the positive control (vitamin E).

2.
J Zhejiang Univ Sci B ; 20(10): 803-815, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31489800

RESUMO

Activated nuclear factor-κB (NF-κB) plays an important role in the development of cardiovascular disease (CVD) through its regulated genes and microRNAs (miRNAs). However, the gene regulation profile remains unclear. In this study, primary mouse vascular endothelial cells (pMVECs) were employed to detect CVD-related NF-κB-regulated genes and miRNAs. Genechip assay identified 77 NF-κB-regulated genes, including 45 upregulated and 32 downregulated genes, in tumor necrosis factor α (TNFα)-treated pMVECs. Ten of these genes were also found to be regulated by NF-κB in TNFα-treated HeLa cells. Quantitative real-time PCR (qRT-PCR) assay confirmed the up-regulation of Egr1, Tnf, and Btg2 by NF-κB in the TNFα-treated pMVECs. The functional annotation revealed that many NF-κB-regulated genes identified in pMVECs were clustered into classical NF-κB-involved biological processes. Genechip assay also identified 26 NF-κB-regulated miRNAs, of which 21 were upregulated and 5 downregulated, in the TNFα-treated pMVECs. Further analysis showed that nine of the identified genes are regulated by seven of these miRNAs. Finally, among the identified NF-κB-regulated genes and miRNAs, 5 genes and 12 miRNAs were associated with CVD by miRWalk and genetic association database analysis. Taken together, these findings show an intricate gene regulation network raised by NF-κB in TNFα-treated pMVECs. The network provides new insights for understanding the molecular mechanism underlying the progression of CVD.

3.
Cell Death Dis ; 10(8): 597, 2019 Aug 08.
Artigo em Inglês | MEDLINE | ID: mdl-31395857

RESUMO

Human adipose-derived stem cells (hADSCs) are increasingly presumed to be a prospective stem cell source for cell replacement therapy in various degenerative and/or traumatic diseases. The potential of trans-differentiating hADSCs into motor neuron cells indisputably provides an alternative way for spinal cord injury (SCI) treatment. In the present study, a stepwise and efficient hADSC trans-differentiation protocol with retinoic acid (RA), sonic hedgehog (SHH), and neurotrophic factors were developed. With this protocol hADSCs could be converted into electrophysiologically active motoneuron-like cells (hADSC-MNs), which expressed both a cohort of pan neuronal markers and motor neuron specific markers. Moreover, after being primed for neuronal differentiation with RA/SHH, hADSCs were transplanted into SCI mouse model and they survived, migrated, and integrated into injured site and led to partial functional recovery of SCI mice. When ablating the transplanted hADSC-MNs harboring HSV-TK-mCherry overexpression system with antivirial Ganciclovir (GCV), functional relapse was detected by motor-evoked potential (MEP) and BMS assays, implying that transplanted hADSC-MNs participated in rebuilding the neural circuits, which was further confirmed by retrograde neuronal tracing system (WGA). GFP-labeled hADSC-MNs were subjected to whole-cell patch-clamp recording in acute spinal cord slice preparation and both action potentials and synaptic activities were recorded, which further confirmed that those pre-conditioned hADSCs indeed became functionally active neurons in vivo. As well, transplanted hADSC-MNs largely prevented the formation of injury-induced cavities and exerted obvious immune-suppression effect as revealed by preventing astrocyte reactivation and favoring the secretion of a spectrum of anti-inflammatory cytokines and chemokines. Our work suggests that hADSCs can be readily transformed into MNs in vitro, and stay viable in spinal cord of the SCI mouse and exert multi-therapeutic effects by rebuilding the broken circuitry and optimizing the microenvironment through immunosuppression.

4.
Plant Physiol ; 2019 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-31451551

RESUMO

The plant pyrethrum (Tanacetum cinerariifolium) synthesizes highly effective natural pesticides known as pyrethrins. Pyrethrins are esters consisting of an irregular monoterpenoid acid and an alcohol derived from jasmonic acid. These alcohols, referred to as rethrolones, can be jasmolone, pyrethrolone, or cinerolone. We recently showed that jasmolone is synthesized from jasmone, a degradation product of jasmonic acid, in a single hydroxylation step catalyzed by jasmone hydroxylase (TcJMH). TcJMH belongs to the CYP71 clade of the cytochrome P450 oxidoreductase family. Here, we used coexpression analysis, heterologous gene expression, and in vitro biochemical assays to identify the enzyme responsible for conversion of jasmolone to pyrethrolone. A further T. cinerariifolium cytochrome P450 family member, CYP82Q3 (designated Pyrethrolone Synthase; PYS), appeared to catalyze the direct desaturation of the C1-C2 bond in the pentyl side chain of jasmolone to produce pyrethrolone. TcPYS is highly expressed in the trichomes of the ovaries in pyrethrum flowers, similar to TcJMH and other T. cinerariifolium genes involved in jasmonic acid biosynthesis. Thus, as previously shown for biosynthesis of the monoterpenoid acid moiety of pyrethrins, rethrolones are synthesized in the trichomes. However, the final assembly of pyrethrins occurs in the developing achenes. Our data provide further insight into pyrethrin biosynthesis, which could ultimately be harnessed to produce this natural pesticide in a heterologous system.

6.
Cell Commun Signal ; 17(1): 109, 2019 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-31462314

RESUMO

BACKGROUND: Breast cancer is the leading cause of cancer-related death in women worldwide. Metastatic disease remains the primary cause of death in patients with breast cancer. Basal-like breast cancer (BLBC) is associated with aggressive behavior, stem-like phenotype, high histological grade, poor clinical features, and high rates of recurrences and/or metastasis. However, the mechanism of BLBC phenotype shaping remains obscure. METHODS: Seventeen normal breast/breast cancer cell lines were used for evaluating the breast cancer subtype-markers, WNT targets and constitutive components, and epithelial mesenchymal transition (EMT) markers analysis by western blot. One hundred and twenty formalin-fixed breast cancer tissues were used for immunohistochemistry (IHC) staining. Nine online platforms (cBioPortal, CCLE, GEPIA, etc.) were used for related analyses. RESULTS: We identified Wnt5b as a key regulatory factor that governs the phenotype of BLBC by activating canonical and non-canonical WNT signaling. Wnt5b exhibited basal-like specificity in cells and clinical samples both at the mRNA and protein levels and also showed good correlation with basal-like phenotype at the mRNA level. Besides, Wnt5b was also a promising therapeutic target for LGK-974 treatment. In addition, we identified that CK1α was expressed at low levels in BLBC and that the activation of CK1α by pyrvinium was an alternative strategy for BLBC treatment. CONCLUSIONS: Wnt5b is not only a diagnostic biomarker but also a potential therapeutic target of BLBC.

7.
Lung Cancer ; 136: 37-44, 2019 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-31437662

RESUMO

PURPOSE: The purpose of this study was to elucidate the clinicopathologic and lymph node metastatic characteristics in patients with adenocarcinoma manifested as persistent ground glass mass (GGM, ground glass opacity [GGO] exceeding 3 cm in diameter). MATERIALS AND METHODS: 304 patients with adenocarcinoma manifested as persistent GGM > 3 cm, who underwent complete surgical resection between November 2013 and December 2017 were included in this study. We elucidated the lymph node metastatic incidence and characteristics according to the primary tumor lobe location and extracted the associated clinicopathological factors, especially thin-section computed tomographic findings, with lymph node involvement. RESULTS: All of the GGMs were invasive adenocarcinoma in histopathology. The total incidence of lymph node metastasis was 2.0% (6/304). All of the 6 cases with hilar or mediastinal lymph node metastasis were manifested as solid-predominant GGM > 3 cm and no cases with lymph node metastasis were identified in GGO-predominant GGM > 3 cm. Lymph node metastases were more likely to present in younger patients (p = 0.032), tumors with solid size >2.0 cm (p = 0.000), more advanced clinical T stage (p = 0.000), radiological solid-predominant tumors (p = 0.002) and acinar-predominant or papillary-predominant adenocarcinoma (p = 0.002). As for solid-predominant GGMs >3 cm, lymph node metastases were more likely to be found in tumors with solid size >2.0 cm (p = 0.026), more advanced clinical T stage(p = 0.026), acinar-predominant or papillary-predominant adenocarcinoma (p = 0.029). Whole tumor size was not associated with the presence of lymph node metastases. There were 2 right upper-lobe cases with upper mediastinal lymph nodes skip metastasis without intrapulmonary, interlobar, and hilar lymph node metastasis. CONCLUSION: All of the GGMs >3 cm were invasive adenocarcinoma. The incidence of lymph node metastasis in GGO-predominant GGMs >3 cm was extremely low. Solid size would be a better predictor of lymph node metastasis than whole tumor size in sold-predominant GGMs >3 cm.

8.
J Org Chem ; 2019 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-31464128

RESUMO

A novel and efficient approach for the synthesis of functionalized isoxazoles via palladium-catalyzed cascade annulation/allylation of alkynyl oxime ethers with allyl halides has been established. The present protocol exhibits mild reaction conditions, good functional group compatibility, and convenient operation. Moreover, scalability was performed and further decoration of the isoxazole product was achieved.

9.
Neurosurg Rev ; 2019 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-31375952

RESUMO

Previous finite element studies of thoracolumbar fractures were mostly based on simulation analysis of one single object, which was difficult to objectively evaluate the differences between conventional pedicle screws and Schanz pedicle screws. The aim of this study was to evaluate the stress of screw and injured vertebrae displacement using the finite element model of conventional pedicle screw and Schanz pedicle screw instrumentation for the treatment of lumbar 1 fractures. Data of eight healthy volunteers were used to simulate the finite element model. The instrumentation models were divided into four groups: moderate fracture conventional (MC), moderate fracture Schanz (MS), unstable/severe fracture conventional (UC), and unstable/severe fracture Schanz (US) pedicle screw groups. The maximum screw stress and lumbar 1 displacement/micro-motion in each group increased with the increase of torque and/or load. Under the same fracture, maximum von Mises stress of conventional pedicle screw (MC/UC) was larger than Schanz pedicle screw (MS/US) (P < 0.05) and lumbar 1 displacement/micro-motion of Schanz pedicle screw (MS/US) was larger than conventional pedicle screw (MC/UC) (P < 0.05). Under the same screws, the maximum von Mises stress and displacement/micro-motion of unstable fracture (UC/US) were larger than moderate fracture (MC/MS) (P < 0.05). Posterior short-segment instrumentation with Schanz pedicle screws were recommended for unstable fractures. The compression displacement/micro-motion of bony defect during flexion may lead to the postoperative re-collapse of injured vertebrae.

10.
Sci Total Environ ; 689: 1-9, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31260894

RESUMO

A generally accepted method to predict selenium (Se) bioavailability of long-term contaminated soils has not yet been established, even if risk assessments in selenosis areas are crucial. In this study, a set of methods were tested to assess the bioavailability of Se to field maize. Fifty maize (Zea mays L.) samples and corresponding soils were collected from a selenosis area (Ziyang, China). The diffusive gradients in thin-films (DGT) technique and the traditional chemical extraction methods, including seven single-step extraction procedures and a five-step sequential extraction were used to predict the bioaccumulation of Se in plant. The result verified the presence of 50% of total Se in the form of residual Se fraction, followed by organic-bound and Fe-Mn oxide-bound Se fractions in soil. In addition, Se6+, Se4+, and Se2- were all detected in the solution extracted by H2O, KCl, phosphate-buffered solution (PBS), NaHCO3, ethylenediaminetetraacetic acid-2Na (EDTA-2Na) and ammonium bicarbonate-diethylenetriaminepentaacetic acid (AB-DTPA), but Se6+ was not extracted by NaOH. The Se extracted by single-step extraction methods was weakly correlated with the Se uptake by plants with relatively high Se concentration (>3 mg·kg-1). The abilities of the tested methods to predict Se bioavailability in naturally Se-enriched soils declined in the following order: DGT > soil solution > PBS > KCl > H2O > NaHCO3 > EDTA > DTPA > NaOH. The ratio of CDGT to soil solution Se (Csoln) totaled 0.13, indicating an extremely low Se supply from the soil solid phase to the soil solution. Se measured by DGT was mainly derived from the soluble and exchangeable Se fractions that can accurately reflect the plant-absorbed Se pool. Therefore, the DGT technique is highly applicable in the simultaneous prediction of Se bioavailability in naturally Se-enriched soils.

11.
Eur J Neurosci ; 2019 Jul 27.
Artigo em Inglês | MEDLINE | ID: mdl-31350860

RESUMO

The priming effect of rewards is a boost in the vigor of reward seeking resulting from the previous receipt of a reward. Extensive work has been carried out on the priming effect of electrical brain stimulation, but much less research exists on the priming effect of natural rewards, such as food. While both reinforcement and motivation are linked with dopamine transmission in the brain, the priming effect of rewards does not appear to be dopamine-dependent. In the present study, an operant method was developed to measure the priming effect of food and then applied to investigate whether it is affected by dopamine receptor antagonism. Long-Evans rats were administered saline or one of the three doses (0.01, 0.05, 0.075 mg/kg) of the dopamine D1 receptor family antagonist, SCH23390, or the dopamine D2 receptor family antagonist, eticlopride. Although dopamine receptor antagonism affected pursuit of food, it did not eliminate the priming effect. These data suggest that despite the involvement of dopamine transmission in reinforcement and motivation, the priming effect of food does not depend on dopamine transmission.

12.
Ann Surg Oncol ; 2019 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-31359275

RESUMO

BACKGROUND: Cholangiocarcinoma (CCA) is a rare, aggressive and highly lethal tumor. The disease is very difficult to diagnose and multi-modality treatments are ineffective. To improve our understanding of the biological characteristics of CCA, and facilitate the identification of valid treatments, an in-depth characterization of two novel Chinese patient-derived primary CCA cell lines was performed. METHODS: Two CCA cell lines were developed and labelled ZJU-0826 and -1125. The two cell lines were characterized with respect to phenotypic, molecular, biomarker, functional and histological properties. RESULTS: Two novel cell lines were cultured for 2 years, and maintained for more than 100 passages. They retained their typical biliary epithelial morphology and ultrastructure. The population doubling times of ZJU-0826, and -1125 were 63.84 h and 44.73 h, respectively. The cells exhibited near-triploid karyotypes with complex structural aberrations. ZJU-1125 cells had mutations in TP53 exons. Short tandem repeats genotyping confirmed the human origin and difference between lines. An immunophenotype analysis showed that ZJU-0826 is positive for CD44, CD29, Pdx1, CD236, FoxA1, FoxA2, and Nanog, and ZJU-1125 positive for CD44, CD29, CD133, Pdx1, FoxA1, FoxA2, and Nanog. ZJU-1125 had greater invasion ability in vitro and tumorigenicity than those of ZJU-0826. CONCLUSIONS: Our results confirm the validity of the ZJU-0826 and -1125 as representative models for the elucidation of the molecular pathogenesis of perihilar CCA, and intrahepatic CCA in both in vitro and in vivo studies, respectively.

13.
Environ Pollut ; 252(Pt B): 1125-1132, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31252110

RESUMO

Knowledge of distribution of toxic metal in crop is essential for studying toxic metal uptake, transportation and bioaccumulation, and it is important for environmental pollution monitoring. In this study, the macro spatial distribution of chromium in rice leaves was visualized by re-heating dual-pulse laser-induced breakdown spectroscopy (DPLIBS) and chemometric methods. After the optimization of two important parameters (delay time and energy ratio) in DPLIBS, chromium prediction model was established based on global spectra. The global model achieved acceptable performance while slight overfitting for model was found because of numerous irrelevant variables. Feature variables including emissions from chromium and other elements were successfully selected by the values of regression coefficient in partial least square regression model. Best performance was achieved by using the feature variables and support vector machine, with correlation coefficient of prediction of 0.959, root mean square error of prediction of 13.4 mg/kg and residual predictive deviation of 3.6. Finally, the distribution of chromium in rice leaves was visualized with the best prediction model. The distribution image showed that chromium distributed approximately symmetrically along the vein and was likely to be accumulated in leaf apex. The preliminary results provide an approach for investigating the macro spatial distribution of elements in crops, which is important for environmental protection and food safety.

14.
Eur Respir J ; 54(2)2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31164430

RESUMO

Although broad knowledge of influenza viral pneumonia has been established, the significance of non-influenza respiratory viruses in community-acquired pneumonia (CAP) and their impact on clinical outcomes remains unclear, especially in the non-immunocompromised adult population.Hospitalised immunocompetent patients with CAP were prospectively recruited from 34 hospitals in mainland China. Respiratory viruses were detected by molecular methods. Comparisons were conducted between influenza and non-influenza viral infection groups.In total, 915 out of 2336 adult patients with viral infection were enrolled in the analysis, with influenza virus (28.4%) the most frequently detected virus, followed by respiratory syncytial virus (3.6%), adenovirus (3.3%), human coronavirus (3.0%), parainfluenza virus (2.2%), human rhinovirus (1.8%) and human metapneumovirus (1.5%). Non-influenza viral infections accounted for 27.4% of viral pneumonia. Consolidation was more frequently observed in patients with adenovirus infection. The occurrence of complications such as sepsis (40.1% versus 39.6%; p=0.890) and hypoxaemia (40.1% versus 37.2%; p=0.449) during hospitalisation in the influenza viral infection group did not differ from that of the non-influenza viral infection group. Compared with influenza virus infection, the multivariable adjusted odds ratios of CURB-65 (confusion, urea >7 mmol·L-1, respiratory rate ≥30 breaths·min-1, blood pressure <90 mmHg (systolic) or ≤60 mmHg (diastolic), age ≥65 years) ≥3, arterial oxygen tension/inspiratory oxygen fraction <200 mmHg, and occurrence of sepsis and hypoxaemia for non-influenza respiratory virus infection were 0.87 (95% CI 0.26-2.84), 0.72 (95% CI 0.26-1.98), 1.00 (95% CI 0.63-1.58) and 1.05 (95% CI 0.66-1.65), respectively. The hazard ratio of 90-day mortality was 0.51 (95% CI 0.13-1.91).The high incidence of complications in non-influenza viral pneumonia and similar impact of non-influenza respiratory viruses relative to influenza virus on disease severity and outcomes suggest more attention should be given to CAP caused by non-influenza respiratory viruses.

15.
Eur J Med Chem ; 178: 623-635, 2019 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-31226654

RESUMO

Glycyrrhetinic acid (GA) had been the star anticancer lead compound and appealed to many scientists all over the world; however, its antitumor activity was not potent enough. To improve GA's cytoxicity and explore the effect of bonding mode on antitumor activity, 32 compounds including GA-OH series (GO, esters in C-3 position) and GA-NH2 series (GN, with amide linkages in C-3 position) had been designed and synthesized. All the compounds were screened for in vitro cytotoxicity against A549, HepG2, MCF-7, Hela and MDCK cell lines. As a result, all the de-protected (without Boc group) derivatives showed much stronger cytotoxic activity than GA, and surprisingly enough, all the GN series of the compounds were more potent than GO series against various tumor cells. Among them, the compound 26 (amide linkages in C-3 position) exhibited stronger antitumor activity against A549 cell line (IC50 = 2.109 ±â€¯0.11 µM) than the positive drug cisplatin (IC50 = 9.001 ±â€¯0.37 µM). Further studies indicated that compound 26 could induce A549 apoptosis via nuclei fragmentation. The detection of apoptosis and cell cycle analysis indicated that compound 26 could induce the early apoptosis and prevent A549 cells transition from S to G2 phase. Furthermore, the structure-activity relationships were briefly discussed. Among which, current study displayed amide linkages in C-3 position could effectively enhance GA cytotoxicity, providing a new modification strategy for further study.

16.
Food Chem ; 295: 327-333, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31174765

RESUMO

Dual-pulse laser-induced breakdown spectroscopy (DPLIBS) and chemometric methods were used to predict chromium content in rice leaves, along with the purpose for increasing the detection sensitivity and accuracy. The influence of important parameters in DPLIBS were investigated and optimized. Then, partial least square (PLS) was used to establish chromium content prediction models, and the value of regression coefficient based on PLS was applied to determine feature variables. In addition, multivariate and univariate analysis were used to verify the modeling performance of selected feature variables. The results indicated that support vector machine model based on feature variables achieved the best performance, with correlation coefficient of 0.9946, root mean square error of 4.85 mg/kg and residual predictive deviation of 9.70 in prediction set. The proposed method provides a high-accuracy and fast approach for chromium content prediction in rice leaves, which could potentially be used for toxic and nutrient elements detection in food.


Assuntos
Cromo/análise , Oryza/química , Folhas de Planta/química , Análise Espectral/métodos , Poluentes Ambientais/análise , Lasers , Análise dos Mínimos Quadrados , Luz , Sensibilidade e Especificidade , Análise Espectral/estatística & dados numéricos , Máquina de Vetores de Suporte
17.
J Biol Chem ; 294(32): 12007-12019, 2019 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-31182483

RESUMO

Cytochrome bc 1 complexes (cyt bc 1), also known as complex III in mitochondria, are components of the cellular respiratory chain and of the photosynthetic apparatus of non-oxygenic photosynthetic bacteria. They catalyze electron transfer (ET) from ubiquinol to cytochrome c and concomitantly translocate protons across the membrane, contributing to the cross-membrane potential essential for a myriad of cellular activities. This ET-coupled proton translocation reaction requires a gating mechanism that ensures bifurcated electron flow. Here, we report the observation of the Rieske iron-sulfur protein (ISP) in a mobile state, as revealed by the crystal structure of cyt bc 1 from the photosynthetic bacterium Rhodobacter sphaeroides in complex with the fungicide azoxystrobin. Unlike cyt bc 1 inhibitors stigmatellin and famoxadone that immobilize the ISP, azoxystrobin causes the ISP-ED to separate from the cyt b subunit and to remain in a mobile state. Analysis of anomalous scattering signals from the iron-sulfur cluster of the ISP suggests the existence of a trajectory for electron delivery. This work supports and solidifies the hypothesis that the bimodal conformation switch of the ISP provides a gating mechanism for bifurcated ET, which is essential to the Q-cycle mechanism of cyt bc 1 function.

18.
Nat Commun ; 10(1): 2482, 2019 06 06.
Artigo em Inglês | MEDLINE | ID: mdl-31171790

RESUMO

Lithium metal based anode with hierarchical structure to enable high rate capability, volume change accommodation, and dendritic suppression is highly desirable for all-solid-state lithium metal battery. However, the fabrication of hierarchical lithium metal based anode is challenging due to the volatility of lithium. Here, we report that natural diatomite can act as an excellent template for constructing hierarchical silicon-lithium based hybrid anode for high performance all-solid-state lithium metal battery. This hybrid anode exhibits stable lithium stripping/plating performance over 1000 h with average overpotential lower than 100 mV without any short circuit. Moreover, all-solid-state full cell using this lithium metal composite anode to couple with lithium iron phosphate cathode shows excellent cycling stability (0.04% capacity decay rate for 500 cycles at 0.5C) and high rate capability (65 mAh g-1 at 5C). The present natural diatomite derived hybrid anode could further promote the fabrication of high performance all-solid-state lithium batteries from sustainable natural resources.

19.
Int J Food Sci Nutr ; : 1-10, 2019 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-31154861

RESUMO

The aim of this study was to evaluate the inhibitory effects of resveratrol (RSV) in A375 and A431 melanoma cells, by assessing cell viability (CCK-8 assay), apoptosis through flow cytometer and cell morphology, cell cycle assay by flow cytometer and western blot (Cyclin D1, Rac1 and PCDH9). Our results demonstrated that RSV strongly inhibited A375 cells proliferation, by decreasing cell viability, promoting apoptosis and arresting cell cycle. Besides, to clarify the main factor - duration or concentration of RSV, the double variance analysis of independent factors was operated after Bartlett's test for homogeneity by R project. According to the outcomes obtained from statistical analyses, Cyclin D1 and PCDH9 were strongly affected by RSV duration while Rac1 was not influenced. In conclusion, RSV can inhibit A375 proliferation and trigger apoptosis by influencing cell cycle proteins; for these effects, treatment duration of RSV played more important role than concentration.

20.
Bioorg Med Chem ; 27(14): 3089-3096, 2019 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-31196755

RESUMO

Aggregation of α-synuclein (α-Syn) play a key role in the development of Parkinson Disease (PD). One of the effective approaches is to stabilize the native, monomeric protein with suitable molecule ligands. We have designed and synthesized a series of sheet-like conjugated compounds which possess different skeletons and various heteroatoms in the two blocks located at both ends of linker, which have good π-electron delocalization and high ability of hydrogen-bond formation. They have shown anti-aggregation activities in vitro towards α-Syn with IC50 down to 1.09 µM. The molecule is found binding in parallel to the NACore within NAC domain of α-Syn, interfering aggregation of NAC region within different α-Syn monomer, and further inhibiting or slowing down the formation of α-Syn oligomer nuclei at lag phase. The potential inhibitor obtained by our strategy is considered to be highly efficient to inhibit α-Syn aggregation.

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