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1.
Front Pharmacol ; 11: 945, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32848720

RESUMO

The radioresistance of tumors affect the outcome of radiotherapy. Accumulating data suggest that 1α,25(OH)2D3 is a potential anti-oncogenic molecule in various cancers. In the present study, we investigated the radiosensitive effects and underlying mechanisms of 1α,25(OH)2D3 in vitro and in vivo. We found that 1α,25(OH)2D3 enhanced the radiosensitivity of lung cancer and ovarian cancer cells by promoting the NADPH oxidase-ROS-apoptosis axis. Compared to the group that only received radiation, the survival fraction and self-renewal capacity of cancer cells treated with a combination of 1α,25(OH)2D3 and radiation were decreased. Both apoptosis and ROS were significantly increased in the combination group compared with the radiation only group. Moreover, N-acetyl-L-cysteine, a scavenger of intracellular ROS, reversed the apoptosis and ROS induced by 1α,25(OH)2D3, indicating that 1α,25(OH)2D3 enhanced the radiosensitivity of cancer cells in vitro by promoting ROS-induced apoptosis. Moreover, our results demonstrated that 1α,25(OH)2D3 promoted the ROS level via activating NADPH oxidase complexes, NOX4, p22phox, and p47phox. In addition, knockdown of the vitamin D receptor (VDR) abolished the radiosensitization of 1α,25(OH)2D3, which confirmed that 1α,25(OH)2D3 radiosensitized tumor cells that depend on VDR. Similarly, our study also evidenced that vitamin D3 enhanced the radiosensitivity of cancer cells in vivo and extended the overall survival of mice with tumors. In summary, these results demonstrate that 1α,25(OH)2D3 enhances the radiosensitivity depending on VDR and activates the NADPH oxidase-ROS-apoptosis axis. Our findings suggest that 1α,25(OH)2D3 in combination with radiation enhances lung and ovarian cell radiosensitivity, potentially providing a novel combination therapeutic strategy.

2.
Hum Pathol ; 76: 58-67, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29555576

RESUMO

Microrchidia 2 (MORC2) plays important roles in DNA damage repair and lipogenesis, but the clinical and functional role of MORC2 in cancer remains largely unexplored. In this study, we showed that MORC2 was widely expressed in human tissues while significantly up-regulated in most cancer types using immunohistochemical staining and analysis of messenger RNA expression profile of more than 2000 human tissue samples from 15 different organs (lung, prostate, liver, breast, brain, stomach, colon/rectum, pancreas, ovary, endometrium, skin, nasopharynx, kidney, esophagus, and bladder). We also found that the MORC2 expression level in high-grade cancer tissues was much more elevated and associated with unfavorable pathological characteristics, poor overall survival, and disease-free survival in several kinds of cancers such as non-small cell lung cancer and breast cancer. Gene set enrichment analysis was used to predict the genes modulated by MORC2, and the results showed that dysregulation of MORC2 in tumor may take part in the cell cycle regulation and genomic instability. We observed that MORC2 knockdown would arrest the cell cycle progress, and the genome of tumors with high MORC2 expression contained more point mutations and gene copy number variation, which validates our gene set enrichment analysis results. The results also showed that MORC2 knockdown would significantly inhibit the proliferation, colony forming, migration, and invasion in multiple cancer cell lines. Taken together, these results highlight the importance of MORC2 in tumorigenesis and cancer progression, and it may act as a potential diagnostic marker and therapeutic target for these diseases.


Assuntos
Biomarcadores Tumorais/metabolismo , Neoplasias/metabolismo , Fatores de Transcrição/metabolismo , Biomarcadores Tumorais/genética , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , China , Intervalo Livre de Doença , Feminino , Perfilação da Expressão Gênica/métodos , Regulação Neoplásica da Expressão Gênica , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Gradação de Tumores , Invasividade Neoplásica , Neoplasias/mortalidade , Neoplasias/patologia , Neoplasias/terapia , RNA Mensageiro/genética , Estudos Retrospectivos , Fatores de Tempo , Fatores de Transcrição/genética , Resultado do Tratamento , Regulação para Cima
3.
Sci Rep ; 7: 40677, 2017 01 17.
Artigo em Inglês | MEDLINE | ID: mdl-28094806

RESUMO

Neurolathyrism is a unique neurodegeneration disease caused by ß-N-oxalyl-L-α, ß- diaminopropionic (ß-ODAP) present in grass pea seed (Lathyrus stativus L.) and its pathogenetic mechanism is unclear. This issue has become a critical restriction to take full advantage of drought-tolerant grass pea as an elite germplasm resource under climate change. We found that, in a human glioma cell line, ß-ODAP treatment decreased mitochondrial membrane potential, leading to outside release and overfall of Ca2+ from mitochondria to cellular matrix. Increased Ca2+ in cellular matrix activated the pathway of ECM, and brought about the overexpression of ß1 integrin on cytomembrane surface and the phosphorylation of focal adhesion kinase (FAK). The formation of high concentration of FA units on the cell microfilaments further induced overexpression of paxillin, and then inhibited cytoskeleton polymerization. This phenomenon turned to cause serious cell microfilaments distortion and ultimately cytoskeleton collapse. We also conducted qRT-PCR verification on RNA-sequence data using 8 randomly chosen genes of pathway enrichment, and confirmed that the data was statistically reliable. For the first time, we proposed a relatively complete signal pathway to neurolathyrism. This work would help open a new window to cure neurolathyrism, and fully utilize grass pea germplasm resource under climate change.


Assuntos
Diamino Aminoácidos/farmacologia , Adesões Focais/efeitos dos fármacos , Adesões Focais/metabolismo , Integrina beta1/metabolismo , Toxinas Biológicas/farmacologia , Cálcio/metabolismo , Linhagem Celular , Biologia Computacional/métodos , Citoesqueleto/metabolismo , Matriz Extracelular , Adesões Focais/genética , Perfilação da Expressão Gênica , Regulação da Expressão Gênica , Ontologia Genética , Redes Reguladoras de Genes , Humanos , Integrina beta1/genética , Latirismo/etiologia , Latirismo/metabolismo , Potencial da Membrana Mitocondrial , Mitocôndrias/metabolismo , Multimerização Proteica , Reprodutibilidade dos Testes , Transdução de Sinais/efeitos dos fármacos , Transcriptoma
4.
Int J Mol Sci ; 17(8)2016 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-27548154

RESUMO

Ovarian cancer is the most lethal gynecological malignancy due to its high metastatic ability. Epithelial-mesenchymal transition (EMT) is essential during both follicular rupture and epithelium regeneration. However, it may also accelerate the progression of ovarian carcinomas. Experimental studies have found that 1α,25-dihydroxyvitamin-D3 [1α,25(OH)2D3] can inhibit the proliferation of ovarian cancer cells. In this study, we investigated whether 1α,25(OH)2D3 could inhibit the migration of ovarian cancer cells via regulating EMT. We established a model of transient transforming growth factor-ß1(TGF-ß1)-induced EMT in human ovarian adenocarcinoma cell line SKOV-3 cells. Results showed that, compared with control, 1α,25(OH)2D3 not only inhibited the migration and the invasion of SKOV-3 cells, but also promoted the acquisition of an epithelial phenotype of SKOV-3 cells treated with TGF-ß1. We discovered that 1α,25(OH)2D3 increased the expression of epithelial marker E-cadherin and decreased the level of mesenchymal marker, Vimentin, which was associated with the elevated expression of VDR. Moreover, 1α,25(OH)2D3 reduced the expression level of transcription factors of EMT, such as slug, snail, and ß-catenin. These results indicate that 1α,25(OH)2D3 suppresses the migration and invasion of ovarian cancer cells by inhibiting EMT, implying that 1α,25(OH)2D3 might be a potential therapeutic agent for the treatment of ovarian cancer.


Assuntos
Colecalciferol/farmacologia , Transição Epitelial-Mesenquimal/efeitos dos fármacos , Neoplasias Ovarianas/metabolismo , Cicatrização/efeitos dos fármacos , Animais , Caderinas/metabolismo , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Feminino , Humanos , Fator de Crescimento Transformador beta1/farmacologia , Vimentina/farmacologia , beta Catenina/metabolismo
5.
Guang Pu Xue Yu Guang Pu Fen Xi ; 36(6): 1755-60, 2016 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-30052386

RESUMO

A fast and efficient way to synthesize a large number of silver nanowires was developed in this paper, in which the reaction conditions were optimized. Under the protection of Cu(NO3)2 silver nitrate was reduced by polyol with polyvinyl pyrrolidone (PVP) in existence. The silver nanowires with uniform structure and good dispersion were obtained. Surface enhancement activity of the silver nanowires was detected by using RhB as a probe molecule,its surface enhancement factor can reach 6.4×105. The results showed that the nanowires significantly enhance the Raman spectroscopy of RhB. The normal Raman spectroscopy (NRS), Raman spectroscopy of D-carnitine solution and Surface enhanced Raman Spectroscopy of D-carnitine by means of the new base were obtained. There are obvious Raman peaks at 3 100~2 800 and 1 700~200 cm-1, and the peak of 1 700~200 cm-1 in the surface enhanced Raman spectra of the D-carnitine can be obviously enhanced. The analysis showed that the angle between the molecular and silver nanoparticles were 180°. The vibrational peaks were assigned comprehensively. Compared with the NRS and SERS of D-carnitine, the detailed structural information of D-carnitine was obtained. In this paper, the surface enhanced Raman spectra of the D-carnitine absorbed on the synthesized silver nanoparticles were obtained, and the minimum detection concentration was 10-6 mol·L-1. The new method can be a rapid and characteristic way to detect D-carnitine, and it will also provide an important guidance for the studies on pharmacology of D-carnitine.

6.
Guang Pu Xue Yu Guang Pu Fen Xi ; 34(11): 2989-93, 2014 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-25752044

RESUMO

Infrared spectroscopy (IR), the normal Raman spectroscopy (NRS) and the surface enhanced Raman spectroscopy (SERS) in new Ag/Cu nanomaterial of Rotundine were studied in the present paper. The IR and the NRS of Rotundine were calculated by the density functional theory (DFT) using B3LYP/6-311+G(d, p), then the spectral intensity graph of Rotundine were given. The vibrational peaks were assigned comprehensively by the visualization software of Gauss view 5. 0. Rotundine has obvious infrared and Raman vibrational peak in the wave number range of 3 300-2500 and 1 800-600 cm(-1). SnCl2 and PVP was used as capping agent for the silver nanoparticles in SERS of Rotundine. Finally, by using the method of cyclic immersion well dispersed silver nanoparticles was obtained and achieved good enhancement effect. This molecule acquired strong selective enhancement vibration peak, In the wave number ranges of 1 500-1 400 and 1 000-700 cm(-1) the enhancement effect is most obvious. After analyzed, the methylene of this molecule is adsorbed on the silver nanoparticles surface and the angle between the benzene ring and the silver substrate is close to 90 degrees. The theoretically calculated spectra of Rotundine are consistent with the obtained experimental spectra. There are some differences may be due to the interaction forces between molecules and so on. The visualization software displayed the structure characteristics and molecular group vibration of this molecular visually and provided important basis for assigning the vibrational peaks. Rotundine is an important traditional Chinese medicine agent contained in many kinds of sedative drugs. The study provides a strong basis for the rapid, feature and trace identification of Rotundine and also supplies important reference for the biological role of central inhibition of analgesic drugs.


Assuntos
Alcaloides de Berberina/química , Espectrofotometria Infravermelho , Análise Espectral Raman , Analgésicos não Entorpecentes/química , Nanopartículas Metálicas , Prata , Software
7.
Guang Pu Xue Yu Guang Pu Fen Xi ; 32(3): 699-702, 2012 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-22582636

RESUMO

Normal Raman spectroscopy and surface-enhanced Raman spectroscopy of aspirin and aspirin tablet were reported, and the vibrational and enhanced peaks were assigned; the interaction of aspirin with DNA was investigated by SERS and UV. The results showed that NRS and SERS of aspirin and aspirin tablet were consistent basically, which indicated that excipient hardly affected the detection of aspirin; in SERS, aspirin was absorbed perpendicularly on silver colloid through the carboxyl group and the benzene ring; The interaction was mainly caused by the inserting-action mode between aspirin and DNA, and the benzene ring and C=O of aspirin were inserted between the base pair of the double helix structure of DNA, which provided important information and useful reference for understanding deeply the mechanism of action of this kind of drug.


Assuntos
Aspirina/química , DNA/química , Espectrofotometria Ultravioleta , Análise Espectral Raman
8.
Bioorg Med Chem ; 20(6): 2119-30, 2012 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-22364952

RESUMO

We wish to report the further design and improved synthesis that resulted in two series of target molecules, TM-1 and TM-2, with remarkably simplified structures containing ß-amino ketone of discrete nabumetone moiety. These were obtained via a 'one-pot, two-step, three-component' protocol of Mannich reaction with yield up to 97%. A total of 28 out of 31 new compounds were characterized using (1)H NMR, (13)C NMR, ESI MS and HRMS techniques. Studies on their antidiabetic activities, screened in vitro at 10 µg mL(-1) level, indicate that TM-2 possesses peroxisome proliferator-activated receptor activation and α-glucosidase inhibition activity significantly stronger than that of TM-1, and also that of the series B compounds that were previously synthesized by the group. Analysis of the structure-activity relationship points to the sulfanilamide unit as the most probable potent group of ß-amino ketone and, on the basis of which, a tangible strategy is presented for the development of new antidiabetic drugs.


Assuntos
Butanonas/química , Butanonas/farmacologia , Hipoglicemiantes/química , Hipoglicemiantes/farmacologia , Cetonas/química , Cetonas/farmacologia , Aminas/síntese química , Aminas/química , Aminas/farmacologia , Butanonas/síntese química , Diabetes Mellitus/tratamento farmacológico , Diabetes Mellitus/metabolismo , Inibidores de Glicosídeo Hidrolases , Humanos , Hipoglicemiantes/síntese química , Cetonas/síntese química , Espectroscopia de Ressonância Magnética , Modelos Moleculares , Nabumetona , Receptores Ativados por Proliferador de Peroxissomo/agonistas , Receptores Ativados por Proliferador de Peroxissomo/metabolismo , Relação Estrutura-Atividade , Sulfanilamida , Sulfanilamidas/síntese química , Sulfanilamidas/química , Sulfanilamidas/farmacologia , alfa-Glucosidases/metabolismo
9.
Guang Pu Xue Yu Guang Pu Fen Xi ; 32(12): 3247-52, 2012 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-23427545

RESUMO

Infrared spectroscopy (IR) and normal Raman spectroscopy (NRS) of Sudan red(III) and Sudan red(IV), and surface -enhanced Raman spectroscopy(SERS) of them on Cu-Ag nanocomposite material were reported in the present paper. The density functional theory (DFT) calculation was applied to calculate IR and NRS on a B3LYP/6-311+G (d, p) level, and the theoretically calculated spectra were carefully compared with the obtained experimental spectra. The vibrational peaks were assigned by the software of Gauss view 5.0. Rapid identification of Sudan red was realized, which provided profitable basis for characteristics and quick test of Sudan dyes, and reliable detection method of food security.

10.
Guang Pu Xue Yu Guang Pu Fen Xi ; 32(12): 3399-401, 2012 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-23427576

RESUMO

A new microwave digestion/inductively coupled plasma mass spectrometry (ICP-MS) for simultaneous determination of 8 heavy metals including Zn, Cu, As, Pb, Cd, Cr, Hg and Ni in wastes of scale hoggery from livestock parks in Chongqing and its biogas slurry after anaerobic digestion, with which an all round, fast analysis of multiple heavy metals in biogas slurry was realized. From the experiment, Zn, Cu, As, Pb, Cd, Cr, Hg, and Ni were the dominating heavy metals in biogas slurry. The linear correlation coefficients for the heavy metals were between 0.999 89 and 0.999 98, indicating a good linear correlation. The determination limits were between 0.79 and 25.0 ng x L(-1), recovery adding standard between 96.5% and 107.6%, RSD between 1.06% and 4.35%. These parameters revealed that the method has good precision and accuracy, which could be applied to the heavy metals detection in biogas slurry, and provide reference for its post-use.


Assuntos
Biocombustíveis/análise , Esterco/análise , Espectrometria de Massas/métodos , Metais Pesados/análise , Suínos , Criação de Animais Domésticos , Animais , Monitoramento Ambiental , Micro-Ondas , Espectrofotometria Atômica/métodos , Gerenciamento de Resíduos
11.
Guang Pu Xue Yu Guang Pu Fen Xi ; 32(10): 2664-8, 2012 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-23285861

RESUMO

Abstract Infrared spectroscopy (FTIR), normal Raman spectroscopy (NRS) of the solids and saturated solutions and surface-enhanced Raman spectroscopy (SERS) on the silver colloidal substrate were reported. The vibrational modes of each group were analyzed, and the enhanced peaks were assigned and compared. The results showed that the major differences between the epimers were the frequency of the CH3 asymmetric stretching vibration and symmetric in-plane deformation vibration of them. According to the mechanism and laws of SERS, the epimers were absorbed on the silver colloid through the P-O bond, carboxyl group and the five-membered ring perpendicularly. The establishment of this adsorption model which realized the rapid identification and the discrimination between the epimers provided important information and useful reference of the adrenal cortical hormone drugs.

12.
Guang Pu Xue Yu Guang Pu Fen Xi ; 32(11): 3068-72, 2012 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-23387181

RESUMO

The degradation of fluoranthene was researched by combined means of multiple fluorescence spectra, including emission, synchronous, excitation emission matrix (EEM), time-scan and photometry. The characteristics of the degradation and fluoranthene molecular changes within the degradation's process were also discussed according to the information about the degradation provided by all of the fluorescence spectra mentioned above. The equations of fluoranthene's degradation by potassium ferrate were obtained on the bases of fitting time-scan fluorescence curves at different time, and the degradation's kinetic was speculated accordingly. From the experimental results, multiple fluorescence data commonly reflected that it had same degradation rate at the same reaction time. t = 10 s, and the degradation rate is -55%, t = 25 s, -81%, t = 40 s, -91%. No new fluorescent characteristic was observed within every degradation' stage. The reaction stage during t < or = 20 s was crucial, in which the degradation process is closest to linear relationship. After this beginning stage, the linear relationship deviated gradually with the development of the degradation process. The degradation of fluoranthene by potassium ferrate was nearly in accord with the order of the first order reaction.

13.
Guang Pu Xue Yu Guang Pu Fen Xi ; 31(5): 1274-8, 2011 May.
Artigo em Chinês | MEDLINE | ID: mdl-21800581

RESUMO

The present paper reports the FT-infrared spectra and FT-Raman spectra of the S-thyminyl-L-cysteine. The optimal concentration of the S-thyminyl-L-cysteine on silver sol is 10(-4) mol L(-1). The interaction of S-thyminyl-L-cysteine with Ag nanoparticles was adsorbed by COO-, NH3+, S, and ring in the tilted way. Amino peak was enhanced in the acidic condition, carboxyl peak was enhanced in alkaline condition. Adsorption of S-thyminyl-L-cysteine on silver nanoparticles was mainly with carboxyl under the acidic condition and with amino under the alkaline condition. Other groups had no change at different pH. Establishment of this adsorption model provided important information and useful reference for further Raman spectra study of PNA, peptides and other biological molecules.


Assuntos
Cisteína/química , Espectroscopia de Infravermelho com Transformada de Fourier , Análise Espectral Raman , Adsorção , Nanopartículas Metálicas , Compostos de Prata
14.
Yao Xue Xue Bao ; 46(4): 412-21, 2011 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-21751495

RESUMO

Twenty five new beta-aminoalcohols containing nabumetone moiety were prepared via the reduction of potassium borohydride with a convenient and efficient procedure, starting from beta-aminoketones that have been synthesized by our group. Their chemical structures were determined by IR, MS, 1H NMR, 13C NMR, HR-MS and antidiabetic activities were screened in vitro. Preliminary results revealed that the antidiabetic activity of most beta-aminoalcohols were better than that of the corresponding beta-aminoketones. Although most compounds showed weak antidiabetic activity, the alpha-glucosidase inhibitory activity of compounds 5hd(1) and 5id(2) reached 74.37% and 90.15%, respectively, which were superior to the positive control. The relative peroxisome proliferator-activated receptor response element (PPRE) activity of five compounds were more than 60%, among them compound 5ca possessed the highest activity (112.59%). As lead molecules of antidiabetic agents, compounds 5hd(1), 5id(2) and 5ca deserve further study.


Assuntos
Amino Álcoois/síntese química , Butanonas/síntese química , Hipoglicemiantes/síntese química , Receptores Ativados por Proliferador de Peroxissomo/metabolismo , alfa-Glucosidases/metabolismo , Amino Álcoois/química , Amino Álcoois/farmacologia , Butanonas/química , Butanonas/farmacologia , Inibidores de Ciclo-Oxigenase 2/síntese química , Inibidores de Ciclo-Oxigenase 2/química , Inibidores de Ciclo-Oxigenase 2/farmacologia , Inibidores de Glicosídeo Hidrolases , Hipoglicemiantes/química , Hipoglicemiantes/farmacologia , Nabumetona , Receptores Ativados por Proliferador de Peroxissomo/agonistas , Elementos de Resposta
15.
Guang Pu Xue Yu Guang Pu Fen Xi ; 30(5): 1376-80, 2010 May.
Artigo em Chinês | MEDLINE | ID: mdl-20672637

RESUMO

The six typical different parts of soils and sediments along the bank of the Three Gorges Reservoir Area (TGRA) were collected, and the humic substance isolated from the six parts of the soils and sediments' samples was separated to humic acid and fulvic acid, purified, and characterized with the combination of the Raman and IR vibrational spectroscopic technologies after cool-dried separation; through assigning the vibrational peaks in each part of the Raman and IR spectra of each sample part, the vibrational characteristics of the structures and the groups that belonged to the molecules of the humic acids and the fulvic acids in the soils and sediments of the TGRA were obtained; the changing features of the groups and structures in the humic acid and the fulvic aicd's molecules from the different soils and sediments in the TGRA were discussed with the environmental impact factors such as soil humic degree, the conditions of different soils conference, using and/or cultivating models and water level fluctuations. From the experimental results, the vibrations about C-O, C-C, and poly-hydrogen bonds dominate in the structures and the groups of each part' humic substance; the active vibration numbers in the upstream are more than in the downstream; the soil's humic degree has great effect on the formation of the humic substances' structures in soil's humic substance; the soil used as agricultural cultivating mode showed higher humic degrees in the upstream parts of the TGRA. The effect of the water level's fluctuation on the formation of the humic acid and fulvic aicd in the sediments of the TGRA is not obvious in the short time.

16.
Guang Pu Xue Yu Guang Pu Fen Xi ; 30(1): 233-8, 2010 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-20302121

RESUMO

The fluorescence emission and excitation emission matrix (EEM) technologies were used to characterize the dissolved organic matter (DOM) in the water body of the Yangtze River and Jialing River around the Chongqing urban areas from April to August 2008. Concerning about the accidents of the Wenchuan's Earthquake in May and Tangjiashan Yansaihu's effects in June, and the high water period time in the summer in two months of July and August, from the EEM obtained from each sampling station and time, the composition, distribution and their changing features of the DOM in the two rivers were investigated as combined with the water samples' environmental parameters such as pH, DO, DOC with EEM's fingerprint features, f(450/500) etc; finally the bio-environment behavior effects of the three types of fluorescence peaks were elaborated, where humic-like, fulvic-like, and protein-like from the five sampling stations' EEMs during the five months were given detailed representation. From the experimental results obtained, the fluorescence peaks are mainly composed of two types of fluorophores: humic-like and protein-like in the two rivers around the Chongqing urban areas during the investigation in five months, the protein-like's peaks value in Jialing River is higher than the values in the Yangtze River, and all the fluorescence peaks in the two Rivers' water body decrease more or less after the two Rivers join in Chun Tan sampling station; the protein-like peak is notably higher after the "5 x 12" earthquake period time including May and June and high water period time, which mainly originated from terrestrial sources, but its intensities decreased observably while the water bodies of the two rivers joining together in the Chao Tianmen and Chun Tan's sampling station.


Assuntos
Monitoramento Ambiental/métodos , Compostos Orgânicos/análise , Rios/química , China , Cidades , Terremotos , Fluorescência , Proteínas/análise , Análise Espectral , Fatores de Tempo
17.
Guang Pu Xue Yu Guang Pu Fen Xi ; 29(11): 2980-3, 2009 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-20101968

RESUMO

FT-Raman and surface enhanced Raman spectra (SERS) of norfloxacin and norfloxacin capsule on the silver colloidal substrate were recorded, and the vibrational and enhanced peaks were assigned; interactions of norfloxacin and DNA were researched. The experimental results proved that the characteristic vibrations of capsule's content and NFX standard, which is v(C-F), vs(O-C-O), v(C=C), did not change, while the changes in molecular skeleton's vibrations were substantial. Additives do not affect the Raman spectroscopy of norfloxacin substantively. An analytical method for detecting norfloxacin medcine can be established by FT-Raman. Without any ions, the interaction is mainly caused by the inserting-action mode between the ring of norfloxacin and the double helix structure of DNA, and norfloxacin could interact with DNA directly, providing reliable evidence for antibacterial mechanism of quinoxalone antibiotics.


Assuntos
DNA/química , Norfloxacino/química , Análise Espectral Raman
18.
Guang Pu Xue Yu Guang Pu Fen Xi ; 28(7): 1559-63, 2008 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-18844160

RESUMO

The authors reported the FT-infrared, and FT-Raman spectra of shikimic acid in solid state and in saturated liquid state in the present article. The vibrational peaks of each group in the molecular structure of shikimic acid were obtained and assigned, and furthermore, the Raman and IR vibrational shifts of the corresponding groups in the molecular structure of shikimic acid were analyzed and speculated especially. As shikimic acid mixed with silver nanoparticle the SERS-active substrate, and was adsorbed on the surface of the silver nanoparticle, we also obtained the surface enhanced Raman scattering (SERS) spectra of shikimic acid at different concentrations from 1.0 x 10(-1) to 1.0 x 10(-5) mol x L(-1), further more the adsorption state and the adsorption characteristics of shikimic acid, adsorbed on the surface of the silver nanoparticle, were studied and speculated with the SERS method and the surface selective rule together. Strong Raman signals were detected in the experiments and each group's vibrations in the molecular structure of shikimic acid were resolved with the combination of FT-infrared and FT-Raman spectroscopy. From the experiment data, the ideal optimized concentrations range is lower than 1 x 10(-3) mol x L, among which we could obtain better SERS spectra. Still, the experimental results suggested that the groups in the molecule structure of shikimic acid, such as COO-, OH-, CH2 and so on, can interact and be absorbed tightly with the surface of silver nanoparticle, and this interaction and absorption were mainly through the SERS charge transfer process mechanism of hydroxyl and carboxyl, and SERS electromagnetic process mechanism of methylene and hypomethyl together with the surface of silver nanopartical. The vibrations of alkene's C=C bond on the ring of cyclohexene in the molecule structure of shikimic showed no obvious enhancement in SERS spectra, which indicated that alkene's C=C bond on the ring of cyclohexene in the molecule structure of shikimic acid had no effective interaction and adsorption on the surface of silver nanoparticle according to the surface selective rule.

19.
Guang Pu Xue Yu Guang Pu Fen Xi ; 27(4): 711-5, 2007 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-17608181

RESUMO

The adsorption orientation and adsorption characteristics of aliphatic-nonpolar amino acids represented by L-methionine on silver nanoparticles were studied by FT-surface-enhanced Raman spectroscopy (FT-SERS) method with different pH and concentrations. According to the experiments, in order to get the best FT-SERS effects, the optimal concentration range of the L-methionine on silver sol is between 10(-3) mol x L(-1)-10(-4)(mol x L(-1). The FT-SERS effects and adsorption model change with pH; The SERS are poor when pH is near the isoelectric point or over 11 or 3. The interaction of L-methionine with Ag nanoparticles is the physical and chemical adsorption by NH3, COO-, S, which could also be effected by pH. From the results and previous reports on this aspect, the general rules of the adsorption model and the orientation of the aliphatic-nonpolar amino acids on silver nanoparticles were speculated, which would be useful for the study of amino acids, protein, peptide, enzyme etc.


Assuntos
Aminoácidos/análise , Nanopartículas Metálicas/química , Prata/química , Análise Espectral Raman/métodos , Adsorção , Concentração de Íons de Hidrogênio , Nanopartículas Metálicas/ultraestrutura , Microscopia Eletrônica de Transmissão
20.
Zoolog Sci ; 23(3): 229-34, 2006 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-16603815

RESUMO

Sixteen polymorphic primers screened from 100 random primers were selected to analyze the randomly amplified polymorphic DNA (RAPD) of 540 domesticated black goats (Capra hircas) from 9 different geographical populations in Sichuan Province of China. After the test, 170 entirely repeatable RAPD markers representing goat polymorphisms were obtained from the 16 polymorphic primers, the lengths of the markers ranging from 0.1 to 2.5 kb. The genetic distance among the black goat populations ranges from 0.1051 to 0.2978. The similarity coefficient (0.9002) between Jintang and Lezhi black goats was the highest in the 9 populations, followed by the coefficient (0.8953) between Jialing and Yinshan goats, while that between Jiangan and Huili goats was found to be the lowest (0.7424). The coefficient of differentiation among population genes (Gst) was 0.2766, indicating a comparatively low degree of differentiation among the black goat populations. A UPGMA dendrogram constructed from similarity coefficients showed that the two populations from Huili and Baiyu, which are found mostly on the Western Sichuan plateau and in mountainous areas, clustered together, and the other seven populations formed another group. It can also be clearly seen that the Huili and Baiyu populations are very special, and must have been closely related in the past, even though their link with the other populations is quite weak as a result of genetic communication. The results of the experiment offer some crucial scientific data useful for the breeding of black goats.


Assuntos
Variação Genética , Genética Populacional , Cabras/classificação , Cabras/genética , Filogenia , Animais , Sequência de Bases , China , Análise por Conglomerados , Primers do DNA , Demografia , Feminino , Amplificação de Genes , Masculino , Peso Molecular , Técnica de Amplificação ao Acaso de DNA Polimórfico
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