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1.
Anal Chim Acta ; 1178: 338847, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34482880

RESUMO

Photodynamic therapy has been generally developed and approved as a promising theranostic technique in recent years, which requires photosensitizers to bear high efficiency of reactive oxygen species production, precisely targeting ability and excellent biocompatibility. The real-time monitoring the microenvironments such as viscosity dynamic involved in mitophagy mediated by photodynamic therapy is significantly important to understand therapeutic process but barely reported. In this work, a pyridinium-functionalized triphenylamine derivative, (E)-4-(2-(4'-(diphenylamino)-[1,1'-biphenyl]-4-yl)vinyl)-1-methylpyridin-1-ium iodide (Mito-I), was exploited as photosensitizer for mitochondria-targeted photodynamic therapy and as fluorescent probe for imaging the mitochondrial viscosity dynamic during mitophagy simultaneously. The results indicated that the additional phenyl ring in Mito-I was beneficial to promote its efficiency of singlet oxygen production. The excellent capability of targeting mitochondria and singlet oxygen generation allowed Mito-I for the specifically mitochondria-targeted photodynamic therapy. Moreover, Mito-I displayed off-on fluorescence response to viscosity with high selectivity and sensitivity. The observed enhancement in fluorescence intensity of Mito-I revealed the increasingly mitochondrial viscosity during mitophagy mediated by the photodynamic therapy of Mito-I. As a result, this work presents a rare example to realize the mitochondria-targeting photodynamic therapy as well as the real-time monitoring viscosity dynamic during mitophagy, which is of great importance for the basic medical research involved in photodynamic therapy.


Assuntos
Mitofagia , Fotoquimioterapia , Mitocôndrias , Fármacos Fotossensibilizantes/farmacologia , Viscosidade
2.
Anal Chem ; 93(35): 12059-12066, 2021 09 07.
Artigo em Inglês | MEDLINE | ID: mdl-34433261

RESUMO

Considering the multiple biological barriers before the entry of photosensitizers (PSs) into cytoplasm, it is of paramount importance to track PSs to elucidate their behaviors and distributions to guide the photodynamic therapy (PDT). Also, the developed PSs suffer from strong oxygen dependency. However, reports on such ideal theranostic platforms are rare. Herein, we developed a theranostic platform (CMTP-2) based on the coumarin-based D-π-A system, which, for the first time, can reveal the holistic intracellular delivery pathway and near-infrared (NIR)-activated mitophagy to guide synergistic type-I PDT and photothermal therapy. The dynamic endo-lysosomal escape of CMTP-2 was monitored, as well as its changeable distributions in endosomes, lysosomes, and mitochondria, demonstrating the preferential accumulation in mitochondria at the end. Upon NIR-I irradiation, CMTP-2 generated toxic radicals and heat, triggering the execution of mitophagy and apoptosis. In vivo experiments on mice indicated that CMTP-2 under 808 nm irradiation realized complete cancer ablation, showing great potential for advancements in synergistic phototherapy.


Assuntos
Mitofagia , Fotoquimioterapia , Animais , Linhagem Celular Tumoral , Lisossomos , Camundongos , Fármacos Fotossensibilizantes/farmacologia , Fármacos Fotossensibilizantes/uso terapêutico , Fototerapia , Terapia Fototérmica
3.
BMC Psychiatry ; 21(1): 330, 2021 07 03.
Artigo em Inglês | MEDLINE | ID: mdl-34217234

RESUMO

BACKGROUND: The outbreak of Coronavirus Disease 2019(COVID-19) caused psychological stress in Chinese adults population. But we are unaware of whether the pandemic causes psychological stress on children. METHODS: We used the Children's Impact of Event Scale questionnaire (CRIES-13) to investigate the degree of Post-traumatic Stress (PTSD) symptoms caused by the pandemic in students selected from schools in Sichuan, Jiangsu, Henan, Yunnan, and Chongqing provinces of China. RESULTS: A total of 7769 students(3692 male and 4077 female), aged 8-18 years, were enrolled in the study, comprising 1214 in primary schools, 2799 in junior high schools and 3756 in senior high schools. A total of 1639 students (21.1%) had severe psychological stress reactions. A large proportion of senior high school students (23.3%) experienced severe psychological stress, and they had the highest median total CRIES-13 score. Female students were more likely to experience severe psychological stress and had higher median CRIES-13 total scores than males. CONCLUSION: COVID-19 has placed psychological stresses on primary and secondary school students in China. These stresses are more likely to reach severe levels among female students and senior high school students.


Assuntos
COVID-19 , Transtornos de Estresse Pós-Traumáticos , Adulto , Criança , China/epidemiologia , Depressão , Surtos de Doenças , Feminino , Humanos , Masculino , SARS-CoV-2 , Instituições Acadêmicas , Transtornos de Estresse Pós-Traumáticos/epidemiologia , Estudantes , Inquéritos e Questionários
4.
PLoS One ; 16(6): e0253311, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34138940

RESUMO

Particleboards are widely used in the artificial board market, which can be constructed from a variety of raw materials and require small amounts of energy to be produced. In the particleboard production process, forming machines play an important role as the key equipment for achieving continuous production. In recent years, airflow forming machines have received increasing attention in particleboard production lines because of their strong separation ability and low price. However, the internal flow field is complex and difficult to control, which affects the surface quality and strength of the particleboard. The most pressing technical difficulty is controlling the flow field characteristics of the airflow paver. At present, the research on this subject is conducted primarily through repeated experiments, which entail long research periods and high processing costs. To reduce human and financial costs, in this study, Computational Fluid Dynamics (CFD) is employed to investigate the flow field and the gas-solid two-phase flow field coupled with particle movement of an airflow forming machine. The accuracy of the calculation model is verified by comparing characteristic point velocities obtained from experimental analysis and a simulation. The simulation results show that in practical production, the frequency of a negative pressure fan should be greater than 27 Hz. It is necessary to set the shoulder properly, and the slab smoothness can be improved by moving the shoulder back on the premise of meeting the strength requirements of the box. The distance between the shoulders of the box body should be less than 2570 mm, and particles with uniform diameter should be added to the paving box to reduce the turbulence effect, improve the quality of particle forming and provide actual particleboard production with a solid theoretical foundation.

5.
Org Lett ; 23(4): 1302-1308, 2021 02 19.
Artigo em Inglês | MEDLINE | ID: mdl-33522830

RESUMO

An "umpolung relay" strategy, which includes an one-pot, twice polarity inversion cascade of C60 via carbanion and carbocation polarity reversed relay pathway, has been developed for the synthesis of a diverse range of novel [60]fulleroindole derivatives.

6.
Inorg Chem ; 60(4): 2362-2371, 2021 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-33494602

RESUMO

Multiphoton materials are in special demand in the field of photodynamic therapy and multiphoton fluorescence imaging. However, rational design methodology for these brands of materials is still nascent. This is despite transition-metal complexes favoring optimized nonlinear-optical (NLO) activity and heavy-atom-effected phosphorescent emission. Here, three four-photon absorption (4PA) platinum(II) complexes (Pt1-Pt3) are achieved by the incorporation of varied functionalized C^N^C ligands with high yields. Pt1-Pt3 exhibit triplet metal-to-ligand charge-transfer transitions at ∼460 nm, which are verified multiple times by transient absorption spectra, time-dependent density functional theory calculations, and low-temperature emission spectra. Further, Pt1-Pt3 undergo 4PA. Notably, one of the complexes, Pt2, has maximum 4PA cross-sectional values of up to 15.2 × 10-82 cm8 s3 photon-3 under excitation of a 1600 nm femtosecond laser (near-IR II window). The 4PA cross sections vary when Pt2 is binding to lecithin and when it displays its lysosome-specific targeting behavior. On the basis of the excellent 4PA property of Pt2, we believe that those 4PA platinum(II) complexes have great potential applications in cancer theranostics.


Assuntos
Antineoplásicos/química , Antineoplásicos/uso terapêutico , Complexos de Coordenação/química , Lisossomos/efeitos dos fármacos , Compostos de Platina/química , Animais , Antineoplásicos/farmacologia , Linhagem Celular Tumoral , Células Cultivadas , Complexos de Coordenação/farmacologia , Complexos de Coordenação/uso terapêutico , Humanos , Camundongos , Fótons , Compostos de Platina/farmacologia , Compostos de Platina/uso terapêutico , Análise Espectral/métodos , Relação Estrutura-Atividade , Ensaios Antitumorais Modelo de Xenoenxerto
7.
J Mater Chem B ; 9(4): 1018-1029, 2021 01 28.
Artigo em Inglês | MEDLINE | ID: mdl-33432955

RESUMO

Recently, phototherapy has attracted much attention due to its negligible invasiveness, insignificant toxicity and excellent applicability. The construction of a newly proposed nanosystem with synergistic photothermal and photodynamic tumor-eliminating properties requires a delicate structure design. In this work, a novel therapeutic nanoplatform (denoted as BCS-Ce6) based on defective cobalt hydroxide nanosheets was developed, which realized hypoxia-relieved photothermal-enhanced photodynamic therapy against cancer. Defective cobalt hydroxide exhibited high photothermal conversion efficacy at the near-infrared region (49.49% at 808 nm) as well as enhanced catalase-like activity to produce oxygen and greatly boost the singlet oxygen generation by a photosensitizer, Ce6, realizing efficacious dual-modal phototherapy. In vivo and in vitro experiments revealed that BCS-Ce6 can almost completely extinguish implanted tumors in a mouse model and present satisfactory biocompatibility during the treatment. This work sets a new angle of preparing photothermal agents and constructing comprehensive therapeutic nanosystems with the ability to modulate the hypoxic tumor microenvironment for efficient cancer therapy.


Assuntos
Antineoplásicos/farmacologia , Hipóxia Celular/efeitos dos fármacos , Nanopartículas/química , Fotoquimioterapia , Fármacos Fotossensibilizantes/farmacologia , Animais , Antineoplásicos/síntese química , Antineoplásicos/química , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Cumarínicos/química , Cumarínicos/farmacologia , Ensaios de Seleção de Medicamentos Antitumorais , Feminino , Células Hep G2 , Humanos , Hidróxidos/química , Hidróxidos/farmacologia , Neoplasias Hepáticas Experimentais/tratamento farmacológico , Neoplasias Hepáticas Experimentais/metabolismo , Neoplasias Hepáticas Experimentais/patologia , Camundongos , Camundongos Endogâmicos ICR , Tamanho da Partícula , Fármacos Fotossensibilizantes/síntese química , Fármacos Fotossensibilizantes/química , Dióxido de Silício/química , Dióxido de Silício/farmacologia , Propriedades de Superfície , Tiazóis/química , Tiazóis/farmacologia , Elementos de Transição/química , Elementos de Transição/farmacologia , Células Tumorais Cultivadas
8.
Drug Dev Res ; 82(3): 422-429, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33231319

RESUMO

While immunotherapy has dramatically revolutionized the treatment of non-small cell lung cancer (NSCLC), it still faces challenges such as low therapeutic efficacy and immune-related adverse events, indicating that safe and effective approaches to enhance NSCLC immunotherapy are still highly demanded. Epidermal growth factor receptor (EGFR) is an established target for molecularly targeted therapy of NSCLC. Overexpression and activating mutations of EGFR can promote the resistance of NSCLC to immunotherapy via upregulating inhibitory immune checkpoints such as programmed death receptor ligand 1 (PD-L1) and indoleamine-2,3-dioxygenase-1 (IDO1). Thus, therapeutic inhibition of EGFR also holds promise in modulating the immune microenvironment to advance NSCLC immunotherapy. In this study, we employed a proteolysis targeting chimera (PROTAC) that degrades EGFRL858R to investigate its potential in dually inhibiting PD-L1 and IDO1 to potentiate the anti-tumor immunity in NSCLC. We demonstrated that PROTAC significantly downregulated the protein levels of both PD-L1 and IDO1 in NSCLC H3255 cell and tumor. We also confirmed that PROTAC significantly suppressed the H3255 tumor growth and enhanced the anti-tumor immune response in H3255 tumor. Overall, our study provides a novel strategy for future NSCLC therapy.

9.
Adv Mater ; 32(39): e2003901, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32815192

RESUMO

The visualization of microtubules by combining optical and electron microscopy techniques provides valuable information to understand correlated intracellular activities. However, the lack of appropriate probes to bridge both microscopic resolutions restricts the areas and structures that can be comprehended within such highly assembled structures. Here, a versatile cyclometalated iridium (III) complex is designed that achieves synchronous fluorescence-electron microscopy correlation. The selective insertion of the probe into a microtubule triggers remarkable fluorescence enhancement and promising electron contrast. The long-life, highly photostable probe allows live-cell super-resolution imaging of tubulin localization and motion with a resolution of ≈30 nm. Furthermore, correlative light-electron microscopy and energy-filtered transmission electron microscopy reveal the well-associated optical and electron signal at a high specificity, with an interspace of ≈41 Å of microtubule monomer in cells.

10.
J Acoust Soc Am ; 148(1): 167, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32752742

RESUMO

The acoustic vortex wave has exhibited high-potential applications such as communication. In this work, the authors propose an efficient method for quantitatively measuring the topological charge of an acoustic vortex through two kinds of annular apertures: an annular triangle aperture and an annular ellipse aperture. It is found that the characteristics of the diffraction pattern in the far-field are well related with the shape of apertures and the topological charges of the acoustic vortex wave. Therefore, the topological charge of acoustic vortex beams can be determined from the diffraction patterns, which provide an effective method for measuring the topological charge of acoustic vortex beams.

11.
J Mater Chem B ; 8(36): 8323-8336, 2020 09 23.
Artigo em Inglês | MEDLINE | ID: mdl-32793936

RESUMO

Exploiting two-dimensional nanomaterials as photo-based theranostic agents is promising for the highly efficient ablation of deep-tissue-buried tumors. However, they are limited by their poor absorption in the second near-infrared-light (NIR-II) bio-window (1000-1300 nm) and intrinsic nonbiodegradability. Herein, defect-rich sulfur-doped Ni(OH)2 (S-Ni(OH)2) nanosheets decorated with bovine serum albumin (BSA) as a novel theranostic agent is developed, which can accomplish multimodal-imaging-guided photothermal ablation of mouse cancers in the NIR-II bio-window. Sulfur doping extends the absorption spectra of Ni(OH)2 nanosheets from the visible to NIR-II bio-window, affording highly efficient photothermal conversion (58.20% for 1064 nm), entailing it to become an excellent contrast agent for photoacoustic imaging. Further, because of their intrinsic paramagnetic property, they can be applied for magnetic resonance imaging. Owing to the abundant defective sites in S-Ni(OH)2 nanosheets, they exhibit response to the tumor microenvironment, resulting in effective biodegradation and excretion from the body. In vivo toxicity experiments indicated that S-Ni(OH)2-BSA NSs delivered no appreciable toxicity and good biocompatibility. This work provides an avenue for the rational design of effective theranostics agents.


Assuntos
Antineoplásicos/uso terapêutico , Hidróxidos/uso terapêutico , Nanoestruturas/uso terapêutico , Neoplasias/diagnóstico por imagem , Neoplasias/tratamento farmacológico , Níquel/uso terapêutico , Microambiente Tumoral/efeitos dos fármacos , Animais , Antineoplásicos/química , Antineoplásicos/farmacocinética , Bovinos , Feminino , Células HeLa , Humanos , Hidróxidos/química , Hidróxidos/farmacocinética , Hidróxidos/efeitos da radiação , Raios Infravermelhos , Camundongos Endogâmicos ICR , Imagem Multimodal , Nanoestruturas/química , Nanoestruturas/efeitos da radiação , Níquel/química , Níquel/farmacocinética , Níquel/efeitos da radiação , Terapia Fototérmica , Soroalbumina Bovina/química , Soroalbumina Bovina/farmacocinética , Soroalbumina Bovina/uso terapêutico , Enxofre/química , Enxofre/farmacocinética , Enxofre/efeitos da radiação , Enxofre/uso terapêutico , Nanomedicina Teranóstica
12.
Chem Commun (Camb) ; 56(66): 9513-9516, 2020 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-32686803

RESUMO

An efficient one-pot three-component domino coupling reaction of phenols, C60, and bromoalkanes was developed, resulting in the highly regioselective synthesis of 1,4-asymmetrical C60 bisadducts. The reaction utilizes KOtBu as a promoter and likely proceeds by an oxyanion/carbanion rearrangement/nucleophilic addition cascade. This new methodology is particularly effective for the synthesis of 1,4-asymmetrical C60 electron transport materials. Its utility is demonstrated by the synthesis of a new efficient 1,4-C60 ETM, which possesses better performance, easier synthesis, and a lower cost compared with the commercially available PCBM.

13.
Cancer Med ; 9(17): 6166-6172, 2020 09.
Artigo em Inglês | MEDLINE | ID: mdl-32657029

RESUMO

BACKGROUND: This study aimed to compare the efficacy and toxicity of raltitrexed (Saiweijian® ) plus cisplatin (SP regimen) and 5-fluorouracil plus cisplatin (FP regimen) as concurrent chemoradiotherapy (CCRT) in patients with locally advanced nasopharyngeal carcinoma (LA-NPC). METHODS: Eligible patients (N = 135) were allocated randomly in a ratio of 1:1 to receive CCRT with either SP or FP. At least 2 cycles of chemotherapy was administrated during radiotherapy. Progression free survival (PFS) was primary endpoint. Secondary endpoints included overall survival (OS), loco-regional relapse free survival (LRRFS), distant metastasis free survival (DMFS) and toxicity. RESULTS: In this study, 68 patients received SP as CCRT, and 67 received FP. Objective responses were noted in 97.1% of the patients in the SP group and in 97.0% of the patients in the FP group (P = 1.00). At the end of a median 36 months follow-up period, the estimated 3-year PFS rates were 70.1% for SP and 66.6% for FP, respectively. The 3-year LRRFS, DMFS and OS rates were 88.9%, 74.7% and 84.0%, respectively, for the SP group, and 92.3%, 71.0% and 73.7%, respectively, for the FP group. Overall, there was no difference between treatment groups with regard to response or survival. The most frequent acute toxicities monitored in both groups were bone marrow suppression, gastrointestinal side effects and oral mucositis (OM). The overall incidence of grade 3-4 OM in the FP group (47.8%) was higher than in the SP group (11.8%). However, the incidence of other adverse effects observed in both groups was similar (P > .05). CONCLUSIONS: These data indicate that SP and FP therapies have similar efficacy in treating LA-NPC. The SP regimen showed a tolerable safety profile along with a lower frequency of severe OM and therefore, an improved life quality. In conclusion, SP was a well tolerated, effective, regimen for LA-NPC treatment.


Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Quimiorradioterapia/métodos , Carcinoma Nasofaríngeo/terapia , Neoplasias Nasofaríngeas/terapia , Adulto , Idoso , Protocolos de Quimioterapia Combinada Antineoplásica/efeitos adversos , Medula Óssea/efeitos dos fármacos , Quimiorradioterapia/efeitos adversos , Distribuição de Qui-Quadrado , Cisplatino/administração & dosagem , Cisplatino/efeitos adversos , Feminino , Fluoruracila/administração & dosagem , Fluoruracila/efeitos adversos , Humanos , Masculino , Pessoa de Meia-Idade , Carcinoma Nasofaríngeo/mortalidade , Carcinoma Nasofaríngeo/patologia , Neoplasias Nasofaríngeas/mortalidade , Neoplasias Nasofaríngeas/patologia , Intervalo Livre de Progressão , Quinazolinas/administração & dosagem , Quinazolinas/efeitos adversos , Estomatite/induzido quimicamente , Tiofenos/administração & dosagem , Tiofenos/efeitos adversos , Adulto Jovem
14.
Anal Chem ; 92(15): 10815-10821, 2020 08 04.
Artigo em Inglês | MEDLINE | ID: mdl-32615754

RESUMO

Cell viability is greatly affected by external stimulus eliciting correlated dynamical physiological processes for cells to choose survival or death. A few fluorescent probes have been designed to detect whether the cell is in survival state or apoptotic state, but monitoring the regulation process of the cell undergoing survival to death remains a long-standing challenge. Herein, we highlight the in situ monitor of mitochondria regulating the cell viability by the RNA-specific fluorescent photosensitizer L. At normal conditions, L anchored mitochondria and interacted with mito-RNA to light up the mitochondria with red fluorescence. With external light stimulus, L generated reactive oxide species (ROS) and cause damage to mitochondria, which activated mitochondrial autophagy to prevent death, during which the red fluorescence of L witnessed dynamical distribution in accordance with the evolution of vacuole structures containing damaged mitochondria into autophagosomes. However, with ROS continuously increasing, the mitochondrial apoptosis was eventually commenced and L with red fluorescent was gradually accumulated in the nucleoli, indicating the programmed cell death. This work demonstrated how the delicate balance between survival and death are regulated by mitochondria.


Assuntos
Mitocôndrias/metabolismo , Fármacos Fotossensibilizantes/farmacologia , RNA/metabolismo , Animais , Apoptose/efeitos dos fármacos , Apoptose/efeitos da radiação , Núcleo Celular/efeitos dos fármacos , Núcleo Celular/metabolismo , Núcleo Celular/efeitos da radiação , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos da radiação , Células HeLa , Humanos , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/efeitos da radiação , Modelos Moleculares , Conformação Molecular , Estresse Oxidativo/efeitos dos fármacos , Estresse Oxidativo/efeitos da radiação , Espécies Reativas de Oxigênio/metabolismo
15.
ACS Appl Mater Interfaces ; 12(2): 1988-1996, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31771326

RESUMO

Photodynamic therapy (PDT) takes advantage of reactive oxygen species (ROS) to trigger the apoptosis for cancer therapy. Given that cell apoptosis is a form of programmed cell death involved with multiple suborganelles and cancer cells are more sensitive to ROS than normal cells, early confirmation of the apoptosis induced by ROS would effectively avoid overtreatment. Herein, we highlight an aggregation-induced emission (AIE)-based theranostic agent (TPA3) to in situ dynamically track mitophagy prior to late apoptosis. TPA3 showed high specificity to autophagy vacuoles (AVs), of which appearance is the signature event of mitophagy during early apoptosis and delivered photocytotoxicity to cancer cells and skin cancer tumors in nude mice under irradiation of white light. Furthermore, in situ monitoring of the dynamical mitophagy process involved with mitochondria, AVs, and lysosomes was performed for the first time under confocal microscopy, providing a real-time self-monitoring system for assessing the curative effect prior to late apoptosis. This fluorescence imaging guided PDT witness great advances for applying in the clinical application.


Assuntos
Apoptose , Mitofagia , Fotoquimioterapia , Nanomedicina Teranóstica , Animais , Apoptose/efeitos da radiação , Autofagossomos/metabolismo , Autofagossomos/efeitos da radiação , Fluorescência , Células HeLa , Humanos , Lisossomos/metabolismo , Lisossomos/efeitos da radiação , Fusão de Membrana/efeitos da radiação , Camundongos Nus , Mitofagia/efeitos da radiação , Imagem Óptica , Espécies Reativas de Oxigênio/metabolismo , Tela Subcutânea/patologia
16.
Spectrochim Acta A Mol Biomol Spectrosc ; 228: 117766, 2020 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-31718977

RESUMO

Lipid droplets (LDs) are organelles featuring in intracellular storage of neutral lipids, which are involved with many biological processes. Monitoring the dynamical cyclic behaviors of cellular LDs poses great importance for early disease diagnosis. Herein, two coumarin-based LDs-specific fluorescence probes exhibited "turn-on" and two-photon fluorescence triggered by breaking their aggregation states. By virtue of establishing oil/water emulsions model to simulate LDs, the behaviors of "turn-on" fluorescence were elucidated, which benefited for the enhancement of selectivity of the probes to LDs. Then, we highlight a LDs-specific coumarin-based two-photon probe (L1) with high photo-stability to monitor the dynamic cyclic behaviors of LDs in cells, unraveling the changes of LDs quantity during lipophagy and the reproduction of LDs to prevent lipotoxicity. We believe the probe offers a convenient way to investigate the biology of LDs.


Assuntos
Corantes Fluorescentes/química , Gotículas Lipídicas/metabolismo , Células HeLa , Células Hep G2 , Humanos
17.
Opt Express ; 27(20): 28337-28349, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31684587

RESUMO

In this paper, the Indices of Polarimetric Purity (IPPs) [1-3] have been proposed to analyze the depolarization performances of mono-dispersion and poly-dispersion scattering systems. Here, we mainly investigate the influences of the particles' density, Refractive Index (RI) of the medium, incident wavelengths, the mixing ratio of bi-dispersion scattering particles and particle-size distributions of poly-dispersion scattering system on the depolarization performances for the backscattering detection. For the mono-dispersion scattering system under same incident wavelength, if the relative RI ratios (m) increase linearly, the depolarization performances of the system will first weaken and then strengthen, and of course, the incident wavelength and density of scattering particles will also influence the depolarization performances of the scattering system. For the bi-dispersion scattering system, the proportion of small particles will be negatively correlated with the depolarization property of the dispersion system, and meanwhile, the particle-size distributions will also affect the depolarization performances greatly in the poly-dispersion scattering system. The results demonstrate that the IPPs can be used to describe the depolarization performances of dispersion systems effectively.

18.
Anal Chem ; 91(23): 14911-14919, 2019 12 03.
Artigo em Inglês | MEDLINE | ID: mdl-31692338

RESUMO

Dynamic tracking of the spatiotemporal coordination among various organelles to in-depth understanding of the mechanism of autophagy have attracted considerable attention. However, the monitor of nucleoli participation in autophagy was somehow neglected. Herein, we report a RNA-targeted bioprobe (ADAP) with high selective permeability into nuclear pore complexes, which induced a two-photon (TP) fluorescence "off-on" response by groove combination with RNA, dynamically monitoring the autophagy process among multiorganelles (nucleoli, mitochondria, and mitochondria-containing lysosomes). This work provides a simple and convenient way to observe the dynamic behavior of multiorganelles during the autophagy process, which benefits the understanding of cellular metabolism.


Assuntos
Técnicas Biossensoriais/métodos , Corantes Fluorescentes/metabolismo , Microscopia de Fluorescência por Excitação Multifotônica/métodos , Poro Nuclear/metabolismo , Fótons , RNA/metabolismo , Autofagia , Nucléolo Celular/metabolismo , Nucléolo Celular/ultraestrutura , Corantes Fluorescentes/síntese química , Células HeLa , Humanos , Lisossomos/metabolismo , Lisossomos/ultraestrutura , Mitocôndrias/metabolismo , Mitocôndrias/ultraestrutura , Mitofagia , Simulação de Acoplamento Molecular , Poro Nuclear/ultraestrutura , Permeabilidade
19.
Theranostics ; 9(8): 2158-2166, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31149035

RESUMO

Platinum complexes have been used for anti-cancer propose for decades, however, their high side effects resulting from damage to healthy cells cannot be neglected and prevent further clinical utilisation. Here, we designed a cyclometalated platinum (II) complex that can bind the endogenous nuclear factor-κB (NF-κB) protein. Employing detailed colocalization studies in co-culture cell line models, we show that by binding to NF-κB, the platinum (II) complex is capable of upregulated nuclear translocation specifically in cancer but not normal cells, thereby impairing cancer proliferation without disturbing healthy cells. In a murine tumour model, the platinum (II) complex prevents tumour growth to a greater extent than cisplatin and with considerably lower side-effects and kidney damage. Considering its weak damage to normal cells combined with high toxicity to cancer cells, this NF-κB-binding platinum complex is a potential anti-cancer candidate and acts to verify the strategy of hijacking endogenous trans-nuclear proteins to achieve cancer-cell specificity and enhance therapeutic indices.


Assuntos
Antineoplásicos/metabolismo , Antineoplásicos/farmacologia , Subunidade p50 de NF-kappa B/metabolismo , Neoplasias/terapia , Compostos de Platina/metabolismo , Compostos de Platina/farmacologia , Nanomedicina Teranóstica/métodos , Transporte Ativo do Núcleo Celular , Animais , Antineoplásicos/administração & dosagem , Sobrevivência Celular/efeitos dos fármacos , Modelos Animais de Doenças , Camundongos , Modelos Biológicos , Transplante de Neoplasias , Compostos de Platina/administração & dosagem , Ligação Proteica , Transplante Heterólogo , Resultado do Tratamento
20.
Anal Chem ; 91(10): 6730-6737, 2019 05 21.
Artigo em Inglês | MEDLINE | ID: mdl-31001974

RESUMO

Unveiling the synergism among multiple organelles for fully exploring the mysteries of the cell has drawn more and more attention. Herein, we developed two two-photon fluorescent bioprobes (Lyso-TA and Mito-QA), of which the conformational change triggered an "off-on" fluorescent response. Lyso-TA can real-time monitor the fusion and movement of lysosomes as well as unveil the mitophagy process with the engagement of lysosomes. Mito-QA was transformed from Lyso-TA by one-step ambient temperature reaction, visualizing the dysfunctional mitochondria through a shift from mitochondria to nucleoli. With superior two-photon absorption cross section, good biocompatibility, and greater penetration depth, two small bioprobes were both applied in in vivo bioimaging of brain tissues and zebrafish.


Assuntos
Acrilonitrila/síntese química , Corantes Fluorescentes/química , Lisossomos/metabolismo , Mitocôndrias/metabolismo , Acrilonitrila/análogos & derivados , Acrilonitrila/efeitos da radiação , Acrilonitrila/toxicidade , Animais , Encéfalo/metabolismo , Nucléolo Celular/metabolismo , Corantes Fluorescentes/síntese química , Corantes Fluorescentes/efeitos da radiação , Corantes Fluorescentes/toxicidade , Células HeLa , Humanos , Microscopia de Fluorescência/métodos , Mitofagia/fisiologia , Conformação Molecular , Fótons , Peixe-Zebra
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