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1.
Neoplasma ; 2020 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-32386477

RESUMO

Hepatitis B virus (HBV) infection is a major public health issue with serious medical consequences. The roles of microRNAs (miRNAs) in HBV replication and expression have been generally recognized, and the abnormal expression of miR-501 has been reported in patients with HBV infection. However, the function and mechanism in HBV replication remain elusive. The expression patterns of miR-501-3p and ZEB2 in HBV-related hepatocellular carcinoma (HCC) tissues and HBV-expressing HCC cells were determined by qRT-PCR and western blot assays. HBV replication and expression were evaluated through detecting the copies of HBV DNA and the secretion of HBV surface antigens HBsAg and HBsAg by real-time PCR and ELISA analyses. Bioinformatics and dual-luciferase reporter analyses were employed to validate the functional interaction between miR-501-3p and ZEB2. MiR-501-3p was significantly upregulated, while ZEB2 was downregulated in HBV-related HCC tissues and cells compared with relative controls without HBV infection. Knockdown of miR-501-3p hampered HBV replication and gene expression in HepG2.2.15 and HepAD38 cells. ZEB2 was identified as a functional target of miR-501-3p. Absence of ZEB2 abolished the inhibitory effects of anti-miR-501-3p on HBV replication and gene expression. Our data indicated that miR-501-3p participated in the regulation of HBV replication and gene expression partially via repressing ZEB2 in HepG2.2.15 and HepAD38 cells, providing a promising antiviral avenue for HBV infection.

2.
Neoplasma ; 2020 Apr 16.
Artigo em Inglês | MEDLINE | ID: mdl-32305053

RESUMO

Long non-coding RNA LINC00261 (LINC00261) has been reported to be implicated in tumorigenesis, treatment, and prognosis in different cancers including non-small cell lung cancer cells (NSCLCs). However, its mechanisms have been poorly investigated in NSCLC. Expressions of LINC00261, miR-19a and Kruppel-like factor 2 (KLF2) were detected using RT-qPCR and western blotting. Cell viability, migration and invasion and apoptosis were detected by MTT assay, transwell assay, flow cytometry and the expressions of Bcl-2, Bax, and cleaved caspase 3 were analyzed by western blotting. Tumor growth in vivo was measured in a xenograft experiment. The target binding between miR-19a and LINC00161 or KLF2 was predicted on the miRcode or Targetscan website and confirmed by luciferase reporter assay. The results showed that LINC00261 was downregulated in NSCLC tumors and cell lines, and this downregulation was correlated with higher TNM stage, larger tumor size, lymph node metastasis, and poor prognosis. Functionally, the upregulation of LINC00261 could promote NSCLC cell apoptosis and inhibit cell viability, migration, and invasion in A549 and H1299 cells, as well as suppress tumor growth. Mechanically, LINC00261 positively regulated KLF2 expression in NSCLC tumors through sponging miR-19a. Expression of miR-19a was upregulated while KLF2 was downregulated in NSCLC tumors, and there existed a negative linear correlation between miR-19a and LINC00261 or KLF2. Rescue experiments demonstrated that both miR-19a upregulation and KLF2 downregulation could abolish the LINC00261 effect in A549 and H1299 cells. Collectively, the upregulation of LINC00261 suppressed NSCLC cell progression through upregulating miR-19a-mediated KLF2, suggesting LINC00261/miR-19a/KLF2 pathway could contribute to the initiation, development, and prognosis in NSCLC.

3.
Zhonghua Liu Xing Bing Xue Za Zhi ; 41(1): 13-19, 2020 Jan 10.
Artigo em Chinês | MEDLINE | ID: mdl-32062936

RESUMO

Objective: To investigate the relationship of sleep duration and sleep quality with anxiety in the elderly aged 60 years and older in China. Methods: The elderly aged 60 years and older were selected from the China Short-term Health Effects of Air Pollution Study conducted between July 18, 2017 and February 7, 2018. Multivariate logistic regression models were used to analyze the association of sleep duration and sleep quality with anxiety. Results: A total of 3 897 elderly aged 60 years and older were included in the study. The age of the elderly was (73.4±8.0) years old. Among the elderly surveyed, 6.5% were defined with anxiety, and 18.7% reported poor sleep quality. Multivariate logistic regression models showed shorter sleep duration was the risk factor for anxiety in the elderly that after adjusting for factors such as general demographics, socioeconomic factors, lifestyle, health status, social support and ambient fine particulates exposure. Compared with the elderly with 7 hours of sleep duration daily, the OR (95%CI) of anxiety for those with sleep duration ≤ 6 hours was 2.09 (1.49-2.93). Compared with those with good sleep quality, the OR (95%CI) of anxiety for those with poor sleep quality was 5.12 (3.88-6.77). We also found statistically significant correlations of the scores of subscales of Pittsburgh sleep quality index with anxiety, in which the effects of sleep disturbance, subjective sleep quality and daytime dysfunction scores were most obvious, the ORs (95%CI) were 4.63 (3.55-6.04), 2.75 (2.33-3.23) and 2.50 (2.19-2.86), respectively. Subgroup analysis showed that the association of sleep duration and sleep quality with anxiety was more obvious in males and in those aged <80 years. Conclusion: Shorter sleep duration and poor sleep quality are associated with anxiety in the elderly in China.


Assuntos
Transtornos de Ansiedade , Transtornos do Sono-Vigília , Sono , Idoso , Idoso de 80 Anos ou mais , Ansiedade , China , Estudos Transversais , Humanos , Masculino , Pessoa de Meia-Idade
4.
Zhonghua Liu Xing Bing Xue Za Zhi ; 41(1): 20-24, 2020 Jan 10.
Artigo em Chinês | MEDLINE | ID: mdl-32062937

RESUMO

Objective: To analyze influencing factors for depressive symptoms in the elderly aged 65 years and older in 8 longevity areas in China. Methods: We recruited 2 180 participants aged 65 years and older in 8 longevity areas from Healthy Aging and Biomarkers Cohort Study, a sub-cohort of the Chinese Longitudinal Healthy Longevity Survey in 2017. Multivariate logistic regression analysis was performed to evaluate the relationships of socio-demographic characteristics, behavioral lifestyle, chronic disease prevalence, functional status, family and social support with depressive symptoms in the elderly. Results: The detection rate of depression symptoms was 15.0% in the elderly aged 65 years and older in 8 longevity areas of China, and the detection rate of depression symptoms was 11.5% in men and 18.5% in women. Multivariate logistic regression analysis results showed that the detection rate of depressive symptoms was lower in the elderly who had regular physical exercises (OR=0.44, 95%CI: 0.26-0.74), frequent fish intakes (OR=0.57, 95%CI: 0.39-0.83), recreational activities (OR=0.65, 95%CI: 0.44-0.96), social activities (OR=0.28, 95%CI: 0.11-0.73) and community services (OR=0.68, 95%CI: 0.50-0.93). The elderly who were lack of sleep (OR=2.04, 95%CI: 1.49-2.80), had visual impairment (OR=1.54, 95%CI: 1.08-2.18), had gastrointestinal ulcer (OR=2.97, 95%CI: 1.53-5.77), had arthritis (OR=2.63, 95%CI: 1.61-4.32), had higher family expenditure than income (OR=1.80, 95%CI: 1.17-2.78) and were in poor economic condition (OR=4.58, 95%CI: 2.48-8.47) had higher detection rate of depressive symptoms. Conclusion: The status of doing physical exercise, fish intake in diet, social activity participation, sleep quality or vision, and the prevalence of gastrointestinal ulcers and arthritis were associated with the detection rate of depressive symptoms in the elderly.


Assuntos
Depressão , Longevidade , Idoso , China , Estudos de Coortes , Estudos Transversais , Feminino , Humanos , Masculino , Prevalência , Fatores de Risco
5.
Zhonghua Liu Xing Bing Xue Za Zhi ; 41(1): 25-30, 2020 Jan 10.
Artigo em Chinês | MEDLINE | ID: mdl-32062938

RESUMO

Objective: To understand the current status of BMI of the elderly and related factors in longevity areas in China, and provide scientific evidence for the control of BMI level in elderly population. Methods: Data used in this study were obtained from Healthy Aging and Biomarkers Cohort Study, a sub-cohort of the Chinese Longitudinal Healthy Longevity Survey. A total of 2 825 elderly in 8 longevity areas in China were surveyed and measured in 2017. The BMI levels of 2 217 elderly aged 65 years and older were calculated and in follow up. The ordered classification logistic regression model was used to analyze the influencd factors for the BMI in the elderly. Results: The BMI of the elderly in 8 longevity areas in China was (22.36±3.87) kg/m(2), and it was (22.76±3.58) kg/m(2) for males and (21.75±3.98) kg/m(2) for females. The BMI levels were normal in 1 165 elderly persons. The prevalence of underweight, overweight and obesity were 15.8%, 24.0% and 7.7%, respectively. Multivariate analysis showed that the main factors affecting the BMI of people under 100- years old were age (65-: OR=2.78, 95%CI: 1.87-4.15; 80-: OR=1.47, 95%CI: 1.00-2.17), smoking status (OR=0.46, 95%CI: 0.32-0.66), annual household income (<30 000 Yuan: OR=1.26, 95%CI: 1.07-1.47; 30 000-70 000 Yuan: OR=1.52, 95%CI: 1.12-1.86), and frequency of tea intake(OR=1.36, 95%CI: 1.01-1.71), while the factor in people aged ≥100 years was gender (OR=3.68, 95%CI: 1.32-10.36). Conclusions: The prevalence of underweight, overweight and obesity were high in the elderly from longevity areas in China. It is necessary to pay attention to the trend of overweight and obesity due to smoking, higher annual household income and regular tea drinking in the elderly men.


Assuntos
Índice de Massa Corporal , Sobrepeso , Idoso , Idoso de 80 Anos ou mais , China , Estudos de Coortes , Feminino , Humanos , Longevidade , Masculino , Fatores de Risco
6.
Zhonghua Liu Xing Bing Xue Za Zhi ; 41(1): 31-35, 2020 Jan 10.
Artigo em Chinês | MEDLINE | ID: mdl-32062939

RESUMO

Objective: To understand the relationship between visual impairment and risk of all-cause mortality in the elderly aged 65 years and older in 8 longevity areas in China. Methods: The data of the elderly aged 65 years and older in the project in 2012 were obtained from Healthy Aging and Biomarkers Cohort Study, a sub-cohort of the Chinese Longitudinal Healthy Longevity Survey, including physical measurement and survival status, and a follow-up for survival outcomes were conducted in 2014 and 2017 respectively. Cox proportional hazard regression model was used to analyze the influence of visual impairment on mortality. Gender and age specific analysis was conducted. Results: A total of 1 736 elderly adults were included. A total of 943 deaths occurred during the 5-year follow-up period with a 5-year mortality rate of 54.3%. The 5-year mortality rate was 76.7% in the group with visual impairment, and 47.6% in the group without visual impairment (P<0.001). After adjusting for demographic information, life style and some disease factors, the risk of 5-year mortality in the group with visual impairment group was 1.30 times higher than that in the group without visual impairment (HR=1.30, 95%CI: 1.09-1.55). In the females, the risk for mortality in the group with visual impairment was 1.48 times higher than that in the group without visual impairment (HR=1.48, 95%CI:1.20-1.84). However, vision status was not associated with the risk for mortality in males (HR=1.02, 95%CI: 0.72-1.43). The risk for mortality in the group with visual impairment was 1.39 times higher than that in the group without visual impairment in the elderly aged over 90 years (HR=1.39, 95%CI: 1.13-1.70). Vision status was not associated with mortality risk in the elderly aged 65-79 years and 80-89 years (HR=1.37, 95%CI: 0.61-3.07; HR=0.95, 95%CI: 0.61-1.48). Conclusion: In the elderly people in China, visual impairment is a risk factor for mortality.


Assuntos
Longevidade , Transtornos da Visão , Adulto , Idoso , Idoso de 80 Anos ou mais , China/epidemiologia , Estudos de Coortes , Feminino , Humanos , Estudos Longitudinais , Masculino , Modelos de Riscos Proporcionais , Fatores de Risco , Transtornos da Visão/mortalidade
7.
Zhonghua Liu Xing Bing Xue Za Zhi ; 41(1): 36-41, 2020 Jan 10.
Artigo em Chinês | MEDLINE | ID: mdl-32062940

RESUMO

Objective: To investigate the association between estimated glomerular filtration rate (eGFR) and all-cause mortality in the elderly aged 65 years and older in longevity areas in China. Methods: Data used in this study were obtained from Healthy Aging and Biomarkers Cohort Study, a sub-cohort of the Chinese Longitudinal Healthy Longevity Survey, 1 802 elderly adults were collected in the study during 2012-2017/2018. In this study, the elderly were classified into 4 groups, moderate-to-severe group [<45 ml·min(-1)·(1.73 m(2))(-1)], mild-to-moderate group [45- ml·min(-1)·(1.73 m(2))(-1)], mild group [60- ml·min(-1)·(1.73 m(2))(-1)] and normal group [≥90 ml·min(-1)·(1.73 m(2))(-1)] according to their eGFR levels. Results: After 6 years of follow-up, 852 participants died, with a mortality rate of 47.3%. Multivariate Cox regression analysis showed that the levels of eGFR were negatively correlated with all-cause mortality risk in the elderly (the HR of elderly was 0.993 and the 95%CI was 0.989-0.997 for every unit of eGFR increased, P=0.001), while compared with the group with normal eGFR, the HRs (95%CI) of the elderly in the moderate-to-severe group, mild-to-moderate group, and mild group were 1.690 (1.224-2.332, P=0.001), 1.312 (0.978-1.758, P=0.070), 1.349 (1.047-1.737, P=0.020) respectively [trend test P<0.001]. Conclusion: The decrease in eGFR was associated with higher mortality risk among the elderly in longevity areas in China.


Assuntos
Taxa de Filtração Glomerular , Longevidade , Idoso , China/epidemiologia , Estudos de Coortes , Humanos , Mortalidade/tendências , Medição de Risco , Fatores de Risco
8.
Zhonghua Liu Xing Bing Xue Za Zhi ; 41(1): 42-47, 2020 Jan 10.
Artigo em Chinês | MEDLINE | ID: mdl-32062941

RESUMO

Objective: To establish a prediction model for 6-year incidence risk of chronic kidney disease (CKD) in the elderly aged 65 years and older in China. Methods: In this prospective cohort study, we used the data of 3 742 participants collected during 2008/2009-2014 and during 2012-2017/2018 from Healthy Aging and Biomarkers Cohort Study, a sub-cohort of the Chinese Longitudinal Healthy Longevity Survey. Two follow up surveys for renal function were successfully conducted for 1 055 participants without CKD in baseline survey. Lasso method was used for the selection of risk factors. The risk prediction model of CKD was established by using Cox proportional hazards regression models and visualized through nomogram tool. Bootstrap method (1 000 resample) was used for internal validation, and the performance of the model was assessed by C-index and calibration curve. Results: The mean age of participants was (80.8±11.4) years. In 4 797 person years of follow up, CKD was found in 262 participants (24.8%). Age, BMI, sex, education level, marital status, having retirement pension or insurance, hypertension prevalence, blood uric acid, blood urea nitrogen and total cholesterol levels and estimated glomerular filtration rate in baseline survey were used in the model to predict the 6-year incidence risk of CKD in the elderly. The corrected C-index was 0.766, the calibration curve showed good consistence between predicted probability and observed probability in high risk group, but relatively poor consistence in low risk group. Conclusion: The incidence risk prediction model of CKD established in this study has a good performance, and the nomogram can be used as visualization tool to predict the 6-year risk of CKD in the elderly aged 65 years and older in China.


Assuntos
Insuficiência Renal Crônica , Idoso , Idoso de 80 Anos ou mais , China/epidemiologia , Estudos de Coortes , Previsões , Taxa de Filtração Glomerular , Humanos , Incidência , Estudos Prospectivos , Insuficiência Renal Crônica/epidemiologia , Fatores de Risco
9.
Artigo em Chinês | MEDLINE | ID: mdl-32074755

RESUMO

Objective: To observe the effect of olfactory training on mice with olfactory dysfunction induced by 3-methylindole (3-MI). Methods: Thirty-one male BALB/c mice were randomly divided into 3 groups by random digits table: control group (group A, n=10), olfactory dysfunction group (group B, n=10) and olfactory dysfunction+olfactory training group (group C, n=11). Mice in group B and group C were intraperitoneally injected with 150 mg/kg 3-MI to induce olfactory dysfunction model, while mice in group A were intraperitoneally injected with corn oil of the same volume. From the first day after injection, mice in group C were treated with 4 kinds of odors by inhalation, while mice in group B were treated with distilled water by inhalation, with 2 times/d, 30 min/time/kind of odor, and continuous training for 28 d. Group A was not treated. Buried food pellet tests were conducted before injection and at 7, 14, 21 and 28 days after injection, respectively. The olfactory epithelium was harvested for observation of the number of olfactory marker protein (OMP) and the thickness of olfactory epithelium on the 28th day after injection. SPSS 23.0 software was used for statistical analysis. Results: Before injection, all mice in each group had no olfactory dysfunction. At the 7th, 14th, 21st and 28th days after injection, the food finding time of mice in group C was shorter than that in group B, and the difference was statistically significant ((175.88±100.50) s vs (266.73±46.83) s, (132.00±84.62) s vs (264.10±48.50) s, (103.57±77.43) s vs (197.43±69.78) s, (67.79±32.54) s vs (176.63±61.06) s, all P<0.05), but food finding time of mice in group B and C was longer than that in group A (the food finding time of group A at the 7th, 14th, 21st and 28th days after injection was (27.13±5.36) s, (25.83±7.28) s, (23.13±2.72) s, (26.63±7.60) s, respectively, all P<0.05). At the 28th day after olfactory training, the number of OMP positive cells in group B and C were fewer than that in group A, and the difference was statistically significant ((108.00±28.19)/HP vs (288.22±84.06)/HP, (199.33±58.55)/HP vs (288.22±84.06)/HP, all P<0.05). The number of OMP positive cells in group C were higher than that in group B (P<0.05). The number of OMP positive cells had negative correlation with food finding time (r=-0.886, P<0.01). As for the thickness of the olfactory epithelium, the thickness of group B was thinner than that in group A and C, and the difference was statistically significant ((59.57±31.27) µm vs (114.55±40.70)µm vs (90.54±37.72) µm, all P<0.05). Conclusion: Olfactory training can accelerate the recovery of olfactory function in 3-MI-induced olfactory impaired mice.


Assuntos
Transtornos do Olfato/terapia , Mucosa Olfatória/fisiopatologia , Animais , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Transtornos do Olfato/induzido quimicamente , Distribuição Aleatória , Olfato
10.
Bioresour Technol ; 301: 122798, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31981907

RESUMO

The polyvinyl formal (PVFM) biocarrier addition in a membrane bioreactor (MBR) was evaluated at high and low carbon/nitrogen (C/N) ratio of 20.0 and 6.7. Results indicated that static biocarrier addition could enrich nitrification and denitrification bacteria, dominating by Tauera, Amaricoccus and Nitrosospira at the genus level and slightly improved the total nitrogen removal even at a low C/N ratio. The bulk sludge characteristics (such as bigger particle size, lower SMP, lower SMP P/C) were also significantly changed in the hybrid MBR (HMBR), leading to a more sustainable membrane operation. The biocarrier addition also reduced the relative abundance of Sphingobacterials_unclassified, Ohtaekwangia and Rhodocyclaceae_unclassified at the genus level, indicating less membrane fouling in the HMBR. Consequently, HMBR with static PVFM addition could partially overcome the drawback of low C/N ratio for total nitrogen removal and membrane fouling control, providing a more resilient MBR to the undesirable environment such as low C/N ratio.


Assuntos
Microbiota , Nitrogênio , Reatores Biológicos , Desnitrificação , Membranas Artificiais , Esgotos , Eliminação de Resíduos Líquidos
11.
Animal ; 14(2): 360-367, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31566174

RESUMO

Some studies have shown that the excessive metabolic heat production is the primary cause for dead chicken embryos during late embryonic development. Increasing heat shock protein (HSP) expression and adjusting metabolism are important ways to maintain body homeostasis under heat stress. This study was conducted to investigate the effects of in ovo injection (IOI) of vitamin C (VC) at embryonic age 11th day (E11) on HSP and metabolic genes expression. A total of 320 breeder eggs were randomly divided into normal saline and VC injection groups. We detected plasma VC content and rectal temperature at chick's age 1st day, and the mRNA levels of HSP and metabolic genes in embryonic livers at E14, 16 and 18, analysed the promoter methylation levels of differentially expressed genes and predicted transcription factors at the promoter regions. The results showed that IOI of VC significantly increased plasma VC content and decreased rectal temperature (P < 0.05). In ovo injection of VC significantly increased heat shock protein 60 (HSP60) and pyruvate dehydrogenase kinase 4 (PDK4) genes expression at E16 and PDK4 and secreted frizzled related protein 1 (SFRP1) at E18 (P < 0.05). At E16, IOI of VC significantly decreased the methylation levels of total CpG sites and -336 CpG site in HSP60 promoter and -1137 CpG site in PDK4 promoter (P < 0.05). Potential binding sites for nuclear factor-1 were found around -389 and -336 CpG sites in HSP60 promoter and potential binding site for specificity protein 1 was found around -1137 CpG site in PDK4 promoter. Our results suggested that IOI of VC increased HSP60, PDK4 and SFRP1 genes expression at E16 and 18, which may be associated with the demethylation in gene promoters. Whether IOI of VC could improve hatchability needs to be further verified by setting uninjection group.


Assuntos
Ácido Ascórbico/administração & dosagem , Galinhas/fisiologia , Regulação da Expressão Gênica no Desenvolvimento/genética , Proteínas de Choque Térmico/genética , Animais , Chaperonina 60/genética , Embrião de Galinha , Galinhas/genética , Feminino , Resposta ao Choque Térmico , Regiões Promotoras Genéticas/genética , Proteínas Quinases/genética , RNA Mensageiro/genética , Distribuição Aleatória
12.
Zhonghua Fu Chan Ke Za Zhi ; 54(12): 848-853, 2019 Dec 25.
Artigo em Chinês | MEDLINE | ID: mdl-31874475

RESUMO

Objective: To study influencing factors which cause the endometrial diseases in patients with breast cancer after operation. Methods: A retrospective study was performed on 212 breast cancer post-operation patients with endometrial diseases between June 2006 and January 2018 in Women's Hospital School of Medicine Zhejiang University to analyse the factors which influenced the endometrial diseases. Results: The abnormal uterine bleeding and endometrial thickness were related to the severity of endometrial disease in patients with breast cancer, and they were independent risk factors for breast cancer patients to have endometrial cancer (P<0.05) . When the diagnostic cut off value of endometrial thickness was ≥0.49 cm, the sensitivity and specificity to endometrial cancer were 78% and 25%, respectively. The average endometrial thickness was (0.56±0.39) cm in patients who were treated by selective estrogen receptor modulator (SERM) after gynecological surgery, which was significantly thicker than that of aromatase inhibitor (AI) group [ (0.33±0.23) cm] and no treatment group [ (0.44±0.28) cm, P<0.05]. The endometrial disease recurrent rate and reoperation rate in SERM group were (26.2%, 14.3%) slightly higher than that of AI group (9.5%, 4.8%) and no treatment group (21.6%, 4.9%), but there were not significant differences (all P>0.05). Conclusions: The clinical symptom of abnormal uterine bleeding and thickening endometrium are risk factors for breast cancer patients to have endometrial cancer. The endometrial thickness has high predictive value for breast cancer patients to diagnose endometrial cancer. The SERM treatment increases the endometrial thickness, recurrent rate and reoperation rate in post-operation patients.


Assuntos
Antineoplásicos Hormonais/efeitos adversos , Inibidores da Aromatase/efeitos adversos , Neoplasias da Mama/tratamento farmacológico , Neoplasias da Mama/cirurgia , Hiperplasia Endometrial/induzido quimicamente , Neoplasias do Endométrio/induzido quimicamente , Endométrio/efeitos dos fármacos , Moduladores de Receptor Estrogênico/efeitos adversos , Moduladores Seletivos de Receptor Estrogênico/efeitos adversos , Hemorragia Uterina/etiologia , Antineoplásicos Hormonais/uso terapêutico , Inibidores da Aromatase/uso terapêutico , Neoplasias da Mama/patologia , Quimioterapia Adjuvante , Terapia Combinada , Hiperplasia Endometrial/epidemiologia , Hiperplasia Endometrial/patologia , Neoplasias do Endométrio/epidemiologia , Neoplasias do Endométrio/patologia , Endométrio/patologia , Moduladores de Receptor Estrogênico/uso terapêutico , Feminino , Humanos , Estudos Retrospectivos , Moduladores Seletivos de Receptor Estrogênico/uso terapêutico , Índice de Gravidade de Doença , Ultrassonografia
13.
Artigo em Chinês | MEDLINE | ID: mdl-31163557

RESUMO

Objective: To compare the short-term outcomes of CO2 laser and conventional laryngeal microsurgery for vocal cord cyst. Method: Patients with vocal cord cyst were divided randomly into two groups. One group was treated with CO2 laser (laser group) and the other underwent Micro-flap surgery(Micro-flap group). For the objective assessment, Amulti-dimensional voice program module for voice spectrum analysis was used. Result: In the laser group, there were no significant differences between the preoperative and 1 week postoperative parameters of Jitter, Shimmer and HNR(P>0.05). However,the parameters of G and VHI-10 were significantly different between the laser group and Micro-flap group(P<0.05). The objective data of the laser group pre-and post-surgery showed that the voice recovery of the laser group was significantly better than that of the Micro-flap group after 1 to 3 months of follow-up(P<0.05). But no significant differences of the parameters of G and VHI-10 was noted between the laser group and Micro-flap group(P>0.05). Conclusion: CO2 laser laryngeal microsurgery for vocal cord cyst can significantly improve pronunciation quality.


Assuntos
Cistos/cirurgia , Doenças da Laringe/cirurgia , Lasers de Gás , Microcirurgia , Prega Vocal/patologia , Qualidade da Voz , Acústica , Humanos , Resultado do Tratamento
14.
Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi ; 33(5): 468-469;473, 2019 May.
Artigo em Chinês | MEDLINE | ID: mdl-31163562

RESUMO

Parapharyngeal space refers to the potential space under skull base between masticatory muscles and pharyngeal muscles, ranging from skull base at the top to hyoid bone at the bottom. The outer lateral wall consists of medial pterygoid muscle, deep parotid lobe and lower jawbone, lateral pharyngeal wall, medial pterygoid, deep lobe of parotid gland and mandible constitute the lateral wall, lateral pharyngeal wall forms medial wall, and prevertebral fascia constitutes the posterior wall, generally forming an inverted pyramid lacuna. Parapharyngeal space is divided into prestyloid space and poststyloid space by stylopharygeal fascia. Prestyloid space is relatively small and contains levator veli palatinetensor veli palatine, branches of maxillary artery, mandibular nerve and its branches. Poststyloid space is relatively large. It includes internal jugular vein, internal carotid artery, posterior cranial nerves, etc. Poststyloid space tumors are relatively rare. In this report, a case of ganglioneuroma wrapping right internal carotid artery is described, which is resected through oral approach.


Assuntos
Ganglioneuroma/cirurgia , Base do Crânio/cirurgia , Artérias Carótidas , Endoscópios , Humanos , Músculos da Mastigação , Pescoço , Músculos Faríngeos , Faringe
15.
Zhonghua Yu Fang Yi Xue Za Zhi ; 53(6): 590-596, 2019 Jun 06.
Artigo em Chinês | MEDLINE | ID: mdl-31177756

RESUMO

Objective: To investigate the relationship of plasma albumin and hypersensitive C-reactive protein (Hs-CRP) with 5-year all-cause mortality among Chinese older adults aged 65 and older. Method: Data was collected in 8 longevity areas of the Chinese Longitudinal Healthy Longevity Survey (CLHLS) study conducted by Chinese Center for Disease Control and Prevention and Peking University at baseline survey in 2012 and 2014, the participants enrolled in 2012 was followed-up in 2014 and 2017, the participants enrolled in 2014 was followed-up in 2017 only. Finally, 3 118 older adults aged 65 and older with complete information on albumin, Hs-CRP and body mass index (BMI) were included in this study. Plasma samples of older adults were collected for the detection of albumin and Hs-CRP at baseline survey. Survival status and follow-up time was recorded for all participants. All older adults were divided into 4 groups according to the levels of plasma albumin and Hs-CRP, and Cox proportional hazard models were constructed to assess their influence on the risk of all-cause mortality. Results: Among 3 118 older adults included, the prevalence of hypoalbuminemia was 10.1% (316/3 118), and was 22.8% (711/3 118) for elevated Hs-CRP. During 10 132 person-years of follow-up, 1 212 participants died. Participants with hypoalbuminemia had increased risk of all-cause mortality, with an hazard ratio (HR) and 95% confidential interval (CI) of 1.18 (1.01-1.38), compared to participants with normal plasma albuminemia; participants with elevated Hs-CRP had increased risk of all-cause mortality, with an HR (95%CI) of 1.18 (1.04-1.35), compared to participants with normal plasma Hs-CRP. Participants with normal plasma albumin and elevated Hs-CRP, with hypoalbuminemia and normal Hs-CRP, with hypoalbuminemia and elevated Hs-CRP also had increased risk of all-cause mortality when compared to those with normal plasma albumin and normal Hs-CRP, the HR (95%CI) were 1.16 (1.01-1.34), 1.11 (0.91-1.37) and 1.43 (1.11-1.83), respectively. Conclusion: Hypoalbuminemia and elevated Hs-CRP were responsible for increased risk of 5-year all-cause mortality among Chinese older adults from 8 longevity areas.


Assuntos
Proteína C-Reativa , Longevidade , Mortalidade , Albumina Sérica , Idoso , Biomarcadores/sangue , Proteína C-Reativa/análise , China , Humanos , Mortalidade/tendências , Fatores de Risco , Albumina Sérica/análise
16.
Eur Rev Med Pharmacol Sci ; 23(8): 3366-3374, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31081091

RESUMO

OBJECTIVE: In recent years, several circular RNAs (circRNAs) have been identified to play important roles in human cancers. However, the exact effects and molecular mechanisms of circRNAs in non-small cell lung cancer (NSCLC) progression are still unknown. In this study, we aimed to investigate the effect of circular hsa_circ_000984 on NSCLC. PATIENTS AND METHODS: Quantitative real-time PCR was performed to detect the expression levels of hsa_circ_000984 in NSCLC tissues and cell lines. The associations between the expression of hsa_circ_000984 and clinicopathological parameters and patients' survival were analyzed. The cell growth was detected by CCK-8 assay and colony formation assay. Cell migration and invasion assays were used to study the changes in cell migration and invasion capacity. Western blot was performed to analyze the possible relationship between hsa_circ_000984 and the genes downstream of the Wnt/ß-catenin pathway. RESULTS: We found that hsa_circ_000984 was highly expressed in NSCLC tissues and cell lines and correlated with advanced TNM stage and lymph nodes metastasis. The clinical assays indicated that patients with high hsa_circ_000984 expression had shorter overall survival and disease-free survival. The functional investigations showed that the knockdown of hsa_circ_000984 suppressed NSCLC cells proliferation, migration, invasion, and EMT. Moreover, hsa_circ_000984 displayed its oncogenic roles by modulating the activation of Wnt/ß-catenin pathway, which was demonstrated by measuring the expression levels of, ß-catenin, c-myc, and cyclin D1. CONCLUSIONS: Our findings may facilitate a better understanding of hsa_circ_000984, and it might be a potential prognostic biomarker and treatment target for NSCLC patients.

17.
Sci Rep ; 9(1): 3642, 2019 Mar 06.
Artigo em Inglês | MEDLINE | ID: mdl-30842466

RESUMO

The state-of-art Si Matel-Oxide-Semiconductor Field-Effect-Transistor (MOS-FET) meets the problem of the Power Consumption (PC) can not be effecively deceased guided by the Moore's Law as before. The GFET has the problem of the device can not be effectively turned off, since the band-gap of the graphene is zero. To solve these problems, noticing the amount of the carriers in the 2 dementional semiconductor material is limited, we propose a Matel-Semi-Insulator-Semiconductor Field-Effect-Transistor (MSIS-FET) to replace the traditional MOS-FET. We verify our idea by fabricating the graphene MSIS-FETs using the natural Aluminium-oxide (Al-oxide) as the semi-insulator gate dielectric. From MSIS-FETs fabricated, we obtain following experimental results. The graphene MSIS-FET is turned off very well, a recorded high Ids on/off ratio of 5 × 107 is achieved. A saddle and close-loop shape transfer feature of Ids-Vgs is obtained first time for transistors. A non-volatile memory characteristics is observed. A carrier re-injection principle and a super-Low PC mechanism for semiconductor devices and integrated circuits (ICs) are found from the transfer feature of the graphene MSIS-FET. It is shown that the PC of the semiconductor devices and (ICs) can be reduced by over three orders of magnitude by using this new mechanism.

19.
Andrology ; 7(2): 220-227, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30575326

RESUMO

BACKGROUND: Semenogelin 1 (SEMG1) is an important secretory protein in spermatozoa involved in the formation of a gel matrix encasing ejaculated spermatozoa. Previous studies show that the SEMG1 gene is highly expressed in spermatozoa from patients with asthenozoospermia (AZS); however, the underlying molecular mechanisms are not yet clear. OBJECTIVES: To study the molecular mechanism of high expression of SEMG1 gene and its potential roles in AZS. MATERIALS AND METHODS: Western blot and real-time PCR were used to detect the expression levels of SEMG1 protein and mRNA in the ejaculated spermatozoa from normozoospermic males and AZS patients. Bioinformatics analysis was used to predict miRNAs targeting for SEMG1 3'-untranslated region detection of the expression levels of all the candidate miRNAs in ejaculatory spermatozoa in AZS patients or normozoospermic volunteers. Luciferase reporter assays were performed to confirm it can directly bind to SEMG1. Correlation of miR-525-3p and SEMG1 mRNA expression with clinical sperm parameters were also analyzed. Finally, we conducted a follow-up study of reproductive history about all the subjects. RESULTS: SEMG1 mRNA and protein level were significantly higher in AZS patients compared to that in normozoospermic volunteers (p < 0.001). Subsequently, microRNA-525-3p (miR-525-3p) which was predicted as a candidate regulator of SEMG1 was found lower expressed in ejaculatory spermatozoa in AZS patients (p = 0.0074). Luciferase experiment revealed that microRNA-525-3p could directly target SEMG1 3'-untranslated region and suppress its expression. Importantly, our retrospective follow-up study showed that both low miR-525-3p expression and high SEMG1 expression level was significantly associated with low progressive sperm motility, abnormal sperm morphology, and infertility. DISCUSSION AND CONCLUSION: The elevated expression of SEMG1 and reduced expression of miR-525-3p are associated with AZS and male infertility. Our study provides a potential therapeutic target for the treatment of male infertility or for male contraception.


Assuntos
Astenozoospermia/genética , Astenozoospermia/metabolismo , MicroRNAs/biossíntese , Proteínas Secretadas pela Vesícula Seminal/biossíntese , Espermatozoides/metabolismo , Adulto , Regulação da Expressão Gênica/genética , Humanos , Masculino , MicroRNAs/genética , Proteínas Secretadas pela Vesícula Seminal/genética
20.
Zhonghua Er Ke Za Zhi ; 56(11): 835-845, 2018 Nov 02.
Artigo em Chinês | MEDLINE | ID: mdl-30392208

RESUMO

Objective: To establish comprehensive laboratory reference intervals for Chinese children. Methods: This was a cross-sectional multicenter study. From June 2013 to December 2014, eligible healthy children aged from 6-month to 17-year were enrolled from 20 medical centers with informed consent. They were assessed by physical examination, questionnaire survey and abdominal ultrasound for eligibility. Fasting blood samples were collected and delivered to central laboratory. Measurements of 15 clinical laboratory parameters were performed, including estradiol (E2), testosterone(T), luteinizing hormone(LH), follicle-stimulating hormone(FSH), alanine transaminase(ALT), serum creatinine(Scr), cystatin C, immunoglobulin A(IgA), immunoglobulin G(IgG), immunoglobulin M(IgM), complement (C3, C4), alkaline phosphatase(ALP), uric acid(UA) and creatine kinase(CK). Reference intervals were established according to central 95% confidence intervals for reference population, stratified by age and sex. Results: In total, 2 259 children were enrolled. Finally, 1 648 children were eligible for this study, including 830 boys and 818 girls, at a mean age of 7.4 years. Age- and sex- specific reference intervals have been established for the parameters. Reference intervals of sex hormones increased gradually with age. Concentrations of ALT, cystatin C, ALP and CK were higher in children under 2 years old. Serum levels of sex hormones, creatinine, immunoglobin, CK, ALP and urea increased rapidly in adolescence, with significant sex difference. In addition, reference intervals were variable depending on assay methods. Concentrations of ALT detected by reagents with pyridoxal 5'-phosphate(PLP) were higher than those detected by reagents without PLP. Compared with enzymatic method, Jaffe assay always got higher results of serum creatinine, especially in children younger than 9 years old. Conclusion: This study established age- and sex- specific reference intervals, for 15 clinical laboratory parameters based on defined healthy children.


Assuntos
Análise Química do Sangue , Valores de Referência , Adolescente , Criança , Pré-Escolar , Estudos Transversais , Estradiol/sangue , Feminino , Hormônio Foliculoestimulante/sangue , Humanos , Lactente , Hormônio Luteinizante/sangue , Masculino
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