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1.
Int J Cancer ; 146(1): 169-180, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31090062

RESUMO

Our previous study demonstrated that heterogeneous nuclear ribonucleoprotein AB (HNRNPAB) is a key gene that facilitates metastasis of hepatocellular carcinoma (HCC). However, the molecular mechanisms behind this relationship are not fully understood. In our study, we utilized long-noncoding RNA (lncRNA) microarrays to identify a HNRNPAB-regulated lncRNA named lnc-ELF209. Our findings from chromatin immunoprecipitation assays indicate that HNRNPAB represses lnc-ELF209 transcription by directly binding to its promoter region. We also analyzed clinical samples from HCC patients and cell lines with quantitative real-time polymerase chain reactions, RNA in situ hybridization and immunohistochemistry, and found that there is a negative relationship between HNRNPAB and lnc-ELF209 expression. Up/downregulation assays and rescue assays indicate that lnc-ELF209 inhibits cell migration, invasion and epithelial-mesenchymal transition regulated by HNRNPAB. This suggests a new regulatory mechanism for HNRNPAB-promoted HCC progression. RNA pull-down and LC-MS/MS were used to determine triosephosphate isomerase, heat shock protein 90-beta and vimentin may be involved in the tumor-suppressed function of lnc-ELF209. Furthermore, we found lnc-ELF209 could stabilize TPI protein expression. We also found that lnc-ELF209 overexpression in HCCLM3 cell resulted in a lower rate of lung metastatic, which suggested a less aggressive HCC phenotype. Collectively, these findings offer new insights into the regulatory mechanisms that underlie HNRNPAB cancer-promoting activities and demonstrate that lnc-ELF209 is a HNRNPAB-regulated lncRNA that may play an important role in the inhibition of HCC progression.

2.
Water Res ; 168: 115145, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31614237

RESUMO

A variety of fluorinated alternatives are being manufactured and applied as a consequence of stringent regulations on legacy poly/perfluoroalkyl substances (PFASs). In this study, 26 emerging and legacy PFASs were measured in the surface water (including dissolved phase and suspended particulate matter) and sediments taken from Hai River basin, China. The total concentrations of PFASs (∑PFASs) ranged from 1.74 to 172 ng/L, with perfluorooctanonate (PFOA) as the dominant compound (15.2% of the ∑PFASs, median value). Emerging PFASs, such as dimer acid of hexafluoropropylene oxide dimer acid (HFPO-DA) and trimer acid (HFPO-TA), were widely detected in the water samples. Specifically, chlorinated polyfluorinated ether sulfonate (F-53B) was observed to be predominant in some sediment samples. A receptor model, Unmix, was introduced to identify the sources of PFASs in the surface water, and the results indicated that fire-fighting foam/fluoropolymer processing aids (36.6%) were the dominant source. The field-based sediment-water (organic carbon normalized) coefficients, Koc, were correlated to the carbon chain lengths of the PFASs. A technique coupling one-way analysis of variance with chemical mass balance model was developed to trace the manufacturing sources of PFOA. Electrochemical fluorination (ECF) was the major PFOA manufacturing source with considerable contribution by telomerization. For the first time, the isomers of perfluorooctane sulfonamide (PFOSA) were quantified in the environmental samples. The lower proportion of branched (br-) PFOSA isomers and higher percentage of br-perfluorooctane sulfonate (PFOS) isomers in the water samples relative to their corresponding commercial products, provided more direct evidences that br-PFOSA isomers were biotransformed more easily than n-PFOSA, explaining the observed enrichment of br-PFOS in the aquatic environment.


Assuntos
Ácidos Alcanossulfônicos , Fluorcarbonetos , Poluentes Químicos da Água , China , Monitoramento Ambiental , Rios
3.
Medicine (Baltimore) ; 98(44): e17686, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31689791

RESUMO

RATIONALE: Ependymomas are neuroepithelial tumors that typically occur in the central nervous system. Ependymomas arising in the mediastinum are exceedingly rare, with only approximately 9 isolated cases reported in the literature to date. PATIENT CONCERNS: A 35-year-old woman was referred to our hospital with complaints of progressive back pain for 3 months. Physical examination revealed decreased breathing sounds and tenderness. Contrast-enhanced computed tomography showed a soft tissue mass with heterogeneous enhancement in the right posterior mediastinum. DIAGNOSES: The diagnosis of primary mediastinal ependymomas (PMEs) was confirmed by postoperative histopathologic examination. INTERVENTIONS AND OUTCOMES: The patient underwent surgical resection of the tumor and experienced local recurrence with neck metastasis 2 years postoperatively. She underwent reoperation for the recurrent tumors and received postoperative radiotherapy and adjuvant chemotherapy. Two years later, the patient is doing well, with no evidence of tumor progression or recurrence. LESSONS: Since PMEs are exceedingly rare, treatment options are limited. Surgical resection seems to be the mainstay of treatment. Further evidence-based studies are required to prove the benefit of radiotherapy and chemotherapy in the treatment of PMEs.

4.
Opt Express ; 27(19): 26471-26482, 2019 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-31674528

RESUMO

Healthcare and biosensing have attracted wide attention worldwide, with the development of chip integration technology in recent decades. In terms of compact sensor design with high performance and high accuracy, photonic crystal structures based on Fano resonance offer superior solutions. Here, we design a photonic crystal structure for sensing applications by proposing modeling for a three-cavity-coupling system and derive the transmission expression based on temporal coupled-mode theory (TCMT). The correlations between the structural parameters and the transmission are discussed. Ultimately, the geometry, composed of an air mode cavity, a dielectric mode cavity and a cavity of wide linewidth, is proved to be feasible for simultaneous sensing of refractive index (RI) and temperature (T). For the air mode cavity, the RI and T sensitivities are 523 nm/RIU and 2.5 pm/K, respectively. For the dielectric mode cavity, the RI and T sensitivities are 145 nm/RIU and 60.0 pm/K, respectively. The total footprint of the geometry is only 14 × 2.6 (length × width) µm2. Moreover, the deviation ratios of the proposed sensor are approximately 0.6% and 0.4% for RI and T, respectively. Compared with the researches lately published, the sensor exhibits compact footprint and high accuracy. Therefore, we believe the proposed sensor will contribute to the future compact lab-on-chip detection system design.

5.
Horm Metab Res ; 51(11): 729-734, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31683343

RESUMO

Contrasting data about the association between proliferative diabetic retinopathy (PDR) and vitamin D status remain unknown. First, a hospital-based cross-sectional study consisting of 889 diabetic retinopathy (DR) and non-DR (NDR) patients was admitted. Further the accumulated evidence was performed to explore the association and dose-response relationship. Our study indicated that the odd ratio for PDR in vitamin D deficiency (VDD) individuals was significantly increased (1.60, 95% CI 1.06-2.42), compared with NDR in vitamin D sufficiency individuals, adjusted by age, sex, diabetic duration, and HbA1c. Four studies plus our study with data on vitamin D levels in 4970 patients with PDR and NDR subjects are compared. Association between vitamin D deficiency and risk of PDR exists (OR=1.69, 95% CI 1.40-2.05; I2=0%, p=0.61). Association between a nonlinear trend for vitamin D decrease with risk of DR was significant (chi2=16.53, p=0.0003). No significant heterogeneity in identified studies was found (goodness of fit chi2=2.98, p=0.225). It is concluded that vitamin D deficiency is significantly associated with risk of proliferative diabetic retinopathy.

6.
Opt Express ; 27(21): 29770-29780, 2019 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-31684234

RESUMO

We report on experimental generation and evolution of circumstance-susceptible, narrow-bandwidth, h-shaped pulse in a thulium-doped fiber (TDF) laser. With typical mode-locking technique based on nonlinear amplifying loop mirror, a type of h-shaped pulse is generated in a net normal dispersion regime for the first time to our best knowledge. Different from pulses with similar profiles achieved in typical anomalous dispersion regime, the h-shaped pulse here exhibits extremely narrow spectral bandwidth and meanwhile becomes highly circumstance-susceptible. Not alike the well-preserved h-shaped profile with anomalous dispersion, here the h-shaped pulse can easily evolve into various other pulse patterns with circumstance variations, including peak-depressed profiles, burst-like emission, multiple h-shaped pulses, and even some highly complex temporal cases. Despite that, the h-shaped pulse broadens as the pump power increasing, being a typical pump-related characteristic dominated by the peak-power-clamping effect. Moreover, it is observed that the h-shaped pulse profile can be re-shaped by incorporating a piece of unpumped TDF into the cavity, i.e., introducing some reabsorption. Our results substantiate the experimental revelation of such a type of particular-profile pulse in the normal dispersion regime, demonstrating some new evolution features facilitated by the dispersion-relevant circumstance-susceptibility.

7.
Biol Pharm Bull ; 42(11): 1953-1956, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31685778

RESUMO

Novel bivalent twin-drug type hydantoin derivatives were evaluated in vitro using a human brain glioma cell line (U251) and a human carcinoma cell line (KB3-1). Among the 5-substituted hydantoin derivatives (1a-b and 2a-d) examined in this study, bivalent symmetrical 5-substituted hydantoin derivative 1b showed the highest anti-proliferative activity towards both U251 and KB3-1 cells. The values of anti-proliferative activity (IC50) of this hydantoin derivative against the two cell lines (U251 and KB3-1) were 0.46 and 5.21 µM, respectively. The anti-proliferative activity of all of the compounds except for compounds 2a and 2d against U251 cells was higher than that of cisplatin. Bivalent symmetrical compound 1b had a biphenylmethane linker in the molecule. All of the tested bivalent hydantoin derivatives showed higher activity against U251 cells than against KB3-1 cells. For twin-drug type hydantoin derivatives 2a-d, which have a linear methylene linker in the molecules, it was found that methylene linker length in these molecules have an effect on the anti-proliferative activity against U251 and KB3-1 cells.

8.
Int Urol Nephrol ; 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31686279

RESUMO

BACKGROUND: Parathyroid glands consist of chief cells and oxyphil cells. The parathyroid oxyphil cell proportion increased dramatically in patients with chronic kidney disease (CKD). The importance of the oxyphil cell in the treatment of secondary hyperparathyroidism (SHPT) has been enhanced in recent years. The aim of this study was to explore the possible relationship between parathyroid oxyphil cell proportion and clinical features of patients with CKD. METHODS: From December 2010 to December 2018, 78 consecutive patients with CKD 5 who underwent parathyroidectomy (PTX) were reviewed. None of the patients had used Cinacalcet. Patients were divided into two groups according to their parathyroid oxyphil cell proportion. The mean parathyroid oxyphil cell proportion of all the patients was obtained. Group 1 consisted of 37 patients, the parathyroid oxyphil cell proportion of whom was more than or equal to mean proportion. Group 2 consisted of 41 patients, the parathyroid oxyphil cell proportion of whom was less than mean proportion. The demographic and biochemical data of the two groups were compared. RESULTS: Preoperative parathyroid hormone (PTH) and total weight of removed parathyroid glands in the patients of group 1were less than those in group 2. There was no difference between the two groups in terms of preoperative calcium (Ca), phosphorus (P), alkaline phosphatase (ALP), age and dialysis years. CONCLUSION: The parathyroid oxyphil cell proportion was associated with preoperative PTH and total weight of removed parathyroid glands in the patients with CKD. Patients with more parathyroid oxyphil proportion had lower preoperative PTH level and smaller total weight of the removed parathyroid glands.

9.
Future Oncol ; 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31729887

RESUMO

Aim: To elucidate the integrative combinational gene regulatory network landscape of hepatocellular carcinoma (HCC) molecular carcinogenesis from diverse background. Materials & methods: Modified gene regulatory network analysis was used to prioritize differentially regulated genes and links. Integrative comparisons using bioinformatics methods were applied to identify potential critical molecules and pathways in HCC with different backgrounds. Results: E2F1 with its surrounding regulatory links were identified to play different key roles in the HCC risk factor dysregulation mechanisms. Hsa-mir-19a was identified as showed different effects in the three HCC differential regulation networks, and showed vital regulatory role in HBV-related HCC. Conclusion: We describe in detail the regulatory networks involved in HCC with different backgrounds. E2F1 may serve as a universal target for HCC treatment.

10.
Opt Lett ; 44(22): 5598-5601, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31730116

RESUMO

We present an experimental realization of a compact and reliable way to build a nondegenerate polarization-entangled photon-pair source based on a dual-periodically-poled $ {\rm Ti}:{{\rm LiNbO}_3} $Ti:LiNbO3 waveguide, which is in the telecommunication window and compatible with the fiber quantum networks. The dual-periodic structure allows two inherently concurrent quasiphase-matching spontaneous parametric down-conversion processes pumped by a single laser beam, hence enabling our source to be compact and stable. We show that our source has a high brightness of $ B = 1.22{\rm } \times {\rm }{10^7}\;{\rm pairs}/(\rm s \times mW \times nm) $B=1.22×107pairs/(s×mW×nm). With quantum state tomography, we estimate an entanglement fidelity of $ 0.945 \pm 0.003 $0.945±0.003. A violation of Clauser-Horne-Shimony-Holt inequality with $ S = 2.75 \pm 0.03 $S=2.75±0.03 is also demonstrated.

11.
Theranostics ; 9(25): 7961-7975, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31695809

RESUMO

Rationale: Aortic dissection (AD) is caused by functional disorder of cells in the aortic wall, which is largely attributed to vascular remodeling. Therapeutic strategies for AD remain limited due to our incomplete understanding of the role of endothelial cells (ECs) in AD pathogenesis. This study aimed to identify the regulatory role of miR-27a in AD and provide a mechanistic basis for a non-invasive treatment of AD. Methods: We harvested aortas from normal and AD patients to explore the expression of miR-27a. In vitro and in vivo assays were preformed to explore the biological effects of differential expression of miR-27a in ECs and its regulatory effect on AD. Results: MiR-27a was lower in intima of AD samples than in healthy individuals. Downregulation of miR-27a in EC was due to up-regulated expression of fas-associated protein with death domain (FADD) and the activation of apoptosis pathway, which led to apoptosis of ECs. Migration of vascular smooth muscle cells was promoted by EC after downregulation of miR-27a due to enhancement of growth/differentiation factor 8 (GDF8) and repression of matrix metalloproteinase-20 (MMP20) in the co-culture system supernatants. Increase in FADD and apoptosis of ECs to induce AD was shown using mouse models of AD in which miR-27a was stably knocked-down by antagomir. Up-regulation of miR-27a by agomir led to a protective effect on AD. Conclusion: Treatment with miR-27a activator that targets apoptosis of ECs strongly diminished occurrence of AD, providing a new strategy for this disease.

12.
Medicine (Baltimore) ; 98(44): e17821, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31689868

RESUMO

Although many genetic variants related to anti-tuberculosis drug induced liver injury (ATDILI) have been identified, the prediction and personalized treatment of ATDILI have failed to achieve, indicating there remains an area for further exploration. This study aimed to explore the influence of single nucleotide polymorphisms (SNPs) in Bradykinin receptor B2 (BDKRB2), Teneurin transmembrane protein 2 (TENM2), transforming growth factor beta 2 (TGFB2), and solute carrier family 2 member 13 (SLC2A13) on the risk of ATDILI.The subjects comprised 746 Chinese tuberculosis (TB) patients. Custom-by-design 2x48-Plex SNPscanTM kit was employed to genotype 28 selected SNPs. The associations of SNPs with ATDILI risk and clinical phenotypes were analyzed according to the distributions of allelic and genotypic frequencies and different genetic models. The odds ratio (OR) with corresponding 95% confidence interval (CI) was calculated.Among subjects with successfully genotyped, 107 participants suffered from ATDILI during follow-up. In BDKRB2, patients with rs79280755 G allele or rs117806152 C allele were more vulnerable to ATDILI (PBonferronicorrection = .002 and .03, respectively). Rs79280755 increased the risk of ATDILI significantly whether in additive (OR = 3.218, 95% CI: 1.686-6.139, PBonferroni correction = .003) or dominant model (PBonferroni correction = .003), as well as rs117806152 (Additive model: PBonferroni correction = .05; dominant model: PBonferroni correction = .03). For TENM2, rs80003210 G allele contributed to the decreased risk of ATDILI (PBonferroni correction = .02), while rs2617972 A allele conferred susceptibility to ATDILI (PBonferroni correction = .01). Regarding rs2617972, significant findings were also observed in both additive (OR = 3.203, 95% CI: 1.487-6.896, PBonferroni correction = .02) and dominant model (PBonferroni correction = .02). Moreover, rs79280755 and rs117806152 in BDKRB2 significantly affected some laboratory indicators. However, no meaningful SNPs were observed in TGFB2 and SLC2A13.Our study revealed that both BDKRB2 and TENM2 genetic polymorphisms were interrogated in relation to ATDILI susceptibility and some laboratory indicators in the Western Chinese Han population, shedding a new light on exploring novel biomarkers and targets for ATDILI.


Assuntos
Antituberculosos/efeitos adversos , Sinalização do Cálcio/genética , Doença Hepática Induzida por Substâncias e Drogas/genética , Proteínas de Membrana/genética , Proteínas do Tecido Nervoso/genética , Receptor B2 da Bradicinina/genética , Adulto , Grupo com Ancestrais do Continente Asiático/genética , Feminino , Marcadores Genéticos , Predisposição Genética para Doença , Proteínas Facilitadoras de Transporte de Glucose/genética , Humanos , Masculino , Pessoa de Meia-Idade , Polimorfismo de Nucleotídeo Único , Estudos Prospectivos , Fator de Crescimento Transformador beta2/genética
13.
Acta Diabetol ; 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31728736

RESUMO

AIMS: This study aimed to explore the level of and changes in the 1,5-anhydroglucitol × glycated hemoglobin A1c/100 (AH index, AHI) associated with different glucose metabolism statuses and to evaluate the islet function and insulin sensitivity of patients with type 2 diabetes (T2DM) with different AHI levels. METHODS: Of the 3562 subjects enrolled in this study, 1697 had T2DM. The disposition index (DI) was the product of islet secretion function and insulin sensitivity-related indexes. RESULTS: The mean AHI level was 1.0 (0.7-1.3) in the general population, while the mean AHI level in the T2DM group was 0.8 (0.5-1.2), which was significantly lower than that in the impaired glucose regulation and normal glucose tolerance group (both 1.2 (0.9-1.5), both P < 0.01). We further divided patients with T2DM into four subgroups according to the quartile of AHI. The results showed that with the increase in AHI level, the homeostasis model assessment of insulin resistance (HOMA-IR) decreased, while HOMA-ß, insulin generation index, insulin sensitivity index, and DI increased (all Pfor trend < 0.01). Multivariate logistic regression showed that the odds ratios for a low DI for increasing levels of AHI were 1.00, 0.22 (0.16-0.29), 0.16 (0.11-0.22), and 0.09 (0.06-0.13), showing a decreasing trend (Pfor trend < 0.05). CONCLUSION: The AHI could reflect the variation in glycemic disorder and the function of islet ß cells. The lower the AHI, the worse the glycemic disorder, as well as the islet ß-cell function.

14.
Artigo em Inglês | MEDLINE | ID: mdl-31729228

RESUMO

Advanced adsorbents need high adsorption rate and superior adsorption capability to clean up the organic methylene blue (MB) from wastewater. We prepared K2Ti8O17 nanowires grown along [0 1 0] direction with a one-step hydrothermal method. The K2Ti8O17 nanowires with tens of nanometers in diameter and tens of micrometers in length were achieved with smooth surfaces and twisted wire-like morphology. The K2Ti8O17 nanowires exhibit high uptake capacity of ∼208.8 mg•g-1 in the MB removal under equilibrium pH = 7. The adsorption equilibriums of MB onto K2Ti8O17 adsorbent is achieved with 97% removal rate MB within only ~21 min, which is the shortest adsorption time among the recent reported inorganic adsorbents towards MB. The adsorption process has a good agreement with the well-known pseudo-second-order kinetic model (k2 = 0.2) and the Langmuir isotherm model. The FTIR measurements suggest that the adsorption can be assigned to the hydrogen bonding and electrostatic attraction between MB and K2Ti8O17. This ultrafast removal ability owns to the larger (0 2 0) interplanar spacing and zigzag surface structure of the nanowires, which provide abundant active adsorption sites. Thermodynamic parameters reflect the spontaneous, exothermic and feasible uptake of MB. Besides, K2Ti8O17 nanowires enjoy high adsorptive ability for chromium (VI) ions and photocatalytic removal towards NO. This work highlights the great significance of K2Ti8O17 nanowires as a low cast promising material used for adsorptive elimanation of organic contaminations in fast water purification on a large scale.

15.
BMC Ophthalmol ; 19(1): 219, 2019 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-31703690

RESUMO

BACKGROUND: The signaling pathway of epithelial to mesenchymal transition (EMT) is regulated by c-Src kinase in many cells. The purpose of this study was to investigate the effects of c-Src kinase on EMT of human lens epithelial cells in vivo stimulated by different factors. METHODS: Human lens epithelial cells, HLE-B3, were exposed to either an inflammatory factor, specifically IL-1α, IL-6, TNF-α or IL-1ß, at 10 ng/mL or high glucose (35.5 mM) for 30 mins. Activity of c-Src kinase was evaluated by the expression of p-Src418 with western blot assay. To investigate the effects of activation of c-Src on EMT, HLE-B3 cells were transfected with pCDNA3.1-SrcY530F to upregulate activity of c-Src kinase, and pSlience4.1-ShSrc to knock it down. The expressions of c-Src kinase and molecular markers of EMT such as E-cadherin, ZO-1, α-SMA, and Vimentin were examined at 48 h by RT-PCR and western blot. At 48 h and 72 h of transfection, cell proliferation was detected by MTT, and cell mobility and migration were determined by scratch and transwell assays. RESULTS: Activity of c-Src kinase, which causes the expression of p-Src418, was upregulated by different inflammatory factors and high glucose in HLE-B3 cells. When HLE-B3 cells were transfected with pCDNA3.1-SrcY530F, the expression of c-Src kinase was upregulated on both mRNA and protein levels, and activity of c-Src kinase, expression of p-Src418 increased. The expressions of both E-cadherin and ZO-1 were suppressed, while the expressions of vimentin and α-SMA were elevated on both mRNA and protein levels at the same time. Cell proliferation, mobility and migration increased along with activation of c-Src kinase. Conversely, when HLE-B3 cells were transfected with pSlience4.1-ShSrc, both c-Src kinase and p-Src418 expressions were knocked down. The expressions of E-cadherin and ZO-1 increased, but the expressions of Vimentin and α-SMA decreased; meanwhile, cell proliferation, mobility and migration reduced. CONCLUSIONS: The c-Src kinase in lens epithelial cells is easily activated by external stimuli, resulting in the induction of cell proliferation, mobility, migration and EMT.

16.
Cancer Genet ; 240: 33-39, 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31726270

RESUMO

Circular RNAs (circRNAs), resulting from the non-canonical splicing of linear pre-mRNAs, have permanently altered our perspectives toward cancer recently, especially in carcinogenesis and cancer progression. However, the roles of circRNAs in esophageal squamous cell carcinoma (ESCC) remain largely unknown. In the current study, circRNAs expression profiles are screened in ESCC, using plasma samples from 10 ESCC patients, including different TNM stages and 5 normal controls. Characteristics of circRNAs including length, types, and the possibility of binding to proteins are analyzed. Candidate tumor-related circRNAs are then quantitated in ESCC tissues, plasmas and cell lines. ESCC tissues can secret circRNAs into plasma and patients with high plasma circ-SLC7A5 are associated with high TNM stage, tending to have shorter overall survival than those with high levels. In addition, the biological characteristics of circ-SLC7A5 including location, miRNAs binding, m6A modification were analyzed. Our study reveals a novel prognosis biomarker of circ-SLC7A5, providing a preliminary landscape of circRNA expression for detection of ESCC.

17.
Sci Total Environ ; 701: 134799, 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31726416

RESUMO

Road accessibility clearly reflects the spatial heterogeneity of urbanization. This study therefore adopted accessibility gradient to analyze the effects of urbanization on Cadmium (Cd) accumulation in agricultural soils. In total, 212 soil samples were collected along the accessibility gradient from agricultural soils in the Guangzhou-Foshan metropolitan region. Cd concentration showed a clearly decreasing pattern in agricultural soils with a decrease in accessibility level. The decreasing patterns varied in different accessibility ranges. The urban-rural ecotone (accessibility range 10-15) was the region with the most drastic changes in Cd accumulation. The influencing factors of Cd accumulation in agricultural soils mainly include industrial pollutants, agriculture chemicals, mining activities, domestic wastes, and soil properties. The importance of these factors varies across different accessibility ranges. Our findings imply that the characteristic variation of Cd accumulation with the road accessibility gradient must be considered in the formulation of targeted policies for controlling Cd contamination in agricultural soils.

18.
Chem Commun (Camb) ; 55(91): 13638-13648, 2019 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-31675019

RESUMO

The judicious incorporation of a fluoroalkyl moiety often brings about beneficial effects on the properties of bioactive molecules. Consequently, efficient methods for selective fluoroalkylation are much sought after in drug discovery. Despite significant achievements in trifluoromethylation, selective mono- and difluoroalkylation is still undeveloped. Catalytic functionalization of fluorinated silyl enol ethers (FSEEs) emerges as a fruitful approach for the diversity-oriented synthesis of value-added α-mono or difluoroalkylated ketones. In this feature article, we detail our efforts in developing catalytic selective mono- and difluoroalkylation reactions using FSEEs. Specifically, we highlight our findings such as activating FSEEs by amines for catalytic enantioselective synthesis, taking advantage of the often observed high activity of FSEEs over the non-fluorinated analogues for reaction development, and the influence of C-FH-X interactions on reactivity and selectivity.

19.
Chem Commun (Camb) ; 2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31713553

RESUMO

Herein, we report that 1,3-diphenylguanidine (DPG) could be utilized for the carboxylative cyclization of homopropargyl amines with CO2 under ambient temperature and pressure, in combination with AgSbF6, which enabled the synthesis of both chiral and achiral 2-oxazinones efficiently. A mechanistic study revealed that the multi-functionality of DPG is critical to the success of the reaction.

20.
Orthop Surg ; 2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31714030

RESUMO

OBJECTIVE: Lumbar spinal stenosis is a medical condition characterized by the narrowing of the spinal canal as a consequence of bone and soft tissue degeneration, including disc herniation, facet and ligamentum flavum hypertrophy, and osteophyte formation. The percutaneous transforaminal endoscopic discectomy (PTED) technique is one of the emerging surgical alternatives for treating central lumbar stenosis. The present study aims to describe the present techniques of PTED and foraminoplasty for central lumbar stenosis, and discuss the feasibility and advantages of this technique. METHODS: A total of 55 patients with an average age of 50 years were recruited in this study. They were operated on between August 2017 and June 2018 by a single surgeon for symptomatic lumbar stenosis using the PTED and foraminoplasty technique, along with a detailed description of the present technique. The retrospective analysis of 55 patients operated between August 2017 and June 2018 by a single surgeon for symptomatic lumbar stenosis using the PTED and foraminoplasty techniques, and the detailed description of the present technique were the focus of the present study. For all patients, the PTED and foraminoplasty procedure was performed under local anesthesia in the lateral position on a radiolucent table using C-arm fluoroscopy. The retrospective analysis evaluated the outcomes of symptoms through follow-up interviews at six weeks, six months, and one year after surgery. The analyzed parameters included surgery time, intraoperative blood loss, postoperative complications, visual analog scale (VAS) score, Japan Orthopedic Association (JOA) score, and the Oswestry Disability Index (ODI). The modified MacNab criteria were adopted. RESULTS: The average duration of symptoms was 15.6 weeks. The mean operative time was 161 minutes. The mean volume of intraoperative blood loss was 21 mL. The mean follow-up period was 14.6 months. The average preoperative VAS score for leg pain and low back pain was 6.8 and 5.5, respectively. The preoperative ODI and JOA score was 49.2 and 14.6, respectively. At the final follow-up, all 55 patients had an average VAS score of 1.1 for leg pain and 0.5 for low back pain. At the same time, the average ODI and JOA score was seven and 24.5, respectively. The statistical analysis showed that the VAS score, ODI value, and JOA score were significantly lower in all time-points at post-operation, when compared to those at pre-operation. For the modified MacNab criteria, the final outcome results were excellent in 39 patients (70.9%), good in nine patients (16.4%), fair in four patients (7.3%), and poor in two patients (3.6%), and the overall success rate was 89.1%. Two patients underwent a second operation during the follow-up period, and their symptoms were released after the reoperation. CONCLUSION: PTED and foraminoplasty technique showed promising outcomes in the treatment of central lumbar stenosis in a 1-year follow-up period. It suggested that PTED and foraminoplasty might be applied as a safe and effective therapeutic option for patients with lumbar stenosis.

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