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1.
Int J Cancer ; 146(1): 169-180, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31090062

RESUMO

Our previous study demonstrated that heterogeneous nuclear ribonucleoprotein AB (HNRNPAB) is a key gene that facilitates metastasis of hepatocellular carcinoma (HCC). However, the molecular mechanisms behind this relationship are not fully understood. In our study, we utilized long-noncoding RNA (lncRNA) microarrays to identify a HNRNPAB-regulated lncRNA named lnc-ELF209. Our findings from chromatin immunoprecipitation assays indicate that HNRNPAB represses lnc-ELF209 transcription by directly binding to its promoter region. We also analyzed clinical samples from HCC patients and cell lines with quantitative real-time polymerase chain reactions, RNA in situ hybridization and immunohistochemistry, and found that there is a negative relationship between HNRNPAB and lnc-ELF209 expression. Up/downregulation assays and rescue assays indicate that lnc-ELF209 inhibits cell migration, invasion and epithelial-mesenchymal transition regulated by HNRNPAB. This suggests a new regulatory mechanism for HNRNPAB-promoted HCC progression. RNA pull-down and LC-MS/MS were used to determine triosephosphate isomerase, heat shock protein 90-beta and vimentin may be involved in the tumor-suppressed function of lnc-ELF209. Furthermore, we found lnc-ELF209 could stabilize TPI protein expression. We also found that lnc-ELF209 overexpression in HCCLM3 cell resulted in a lower rate of lung metastatic, which suggested a less aggressive HCC phenotype. Collectively, these findings offer new insights into the regulatory mechanisms that underlie HNRNPAB cancer-promoting activities and demonstrate that lnc-ELF209 is a HNRNPAB-regulated lncRNA that may play an important role in the inhibition of HCC progression.

2.
Water Res ; 168: 115145, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31614237

RESUMO

A variety of fluorinated alternatives are being manufactured and applied as a consequence of stringent regulations on legacy poly/perfluoroalkyl substances (PFASs). In this study, 26 emerging and legacy PFASs were measured in the surface water (including dissolved phase and suspended particulate matter) and sediments taken from Hai River basin, China. The total concentrations of PFASs (∑PFASs) ranged from 1.74 to 172 ng/L, with perfluorooctanonate (PFOA) as the dominant compound (15.2% of the ∑PFASs, median value). Emerging PFASs, such as dimer acid of hexafluoropropylene oxide dimer acid (HFPO-DA) and trimer acid (HFPO-TA), were widely detected in the water samples. Specifically, chlorinated polyfluorinated ether sulfonate (F-53B) was observed to be predominant in some sediment samples. A receptor model, Unmix, was introduced to identify the sources of PFASs in the surface water, and the results indicated that fire-fighting foam/fluoropolymer processing aids (36.6%) were the dominant source. The field-based sediment-water (organic carbon normalized) coefficients, Koc, were correlated to the carbon chain lengths of the PFASs. A technique coupling one-way analysis of variance with chemical mass balance model was developed to trace the manufacturing sources of PFOA. Electrochemical fluorination (ECF) was the major PFOA manufacturing source with considerable contribution by telomerization. For the first time, the isomers of perfluorooctane sulfonamide (PFOSA) were quantified in the environmental samples. The lower proportion of branched (br-) PFOSA isomers and higher percentage of br-perfluorooctane sulfonate (PFOS) isomers in the water samples relative to their corresponding commercial products, provided more direct evidences that br-PFOSA isomers were biotransformed more easily than n-PFOSA, explaining the observed enrichment of br-PFOS in the aquatic environment.


Assuntos
Ácidos Alcanossulfônicos , Fluorcarbonetos , Poluentes Químicos da Água , China , Monitoramento Ambiental , Rios
3.
J Phys Condens Matter ; 32(6): 065501, 2020 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-31627194

RESUMO

The atomically thin group III monochalcogenides have emerged as potential candidates for nanoscale optoelectronic applications due to their tunable bandgaps and high carrier mobility. In this work, by means of ab initio calculations, we have systematically investigated the geometrical structures, electronic structures, and optical properties of the orthogonal phase (O-phase) group III monochalcogenides MX (M = Ga and In, X = S, Se and Te) monolayers, nanoribbons, heterostructures and their potential applications as photocatalysts for water splitting. It is highlighted that the two-dimensional (2D) O-phase MX monolayers not only are dynamically and thermally stable, but also exhibit distinguished optical properties (~105 cm-1) with broad absorption spectrum in the visible and ultraviolet light regions. Furthermore, it is noted that nano-structure designing can further modulate their electronic structures in desirable ways. For instance, the bandgap of O-phase GaTe is relevant to the width of 1D nanoribbon. On the other hand, the type-II InSe/GaTe and InTe/GaTe heterostructures confine the photo-generated electrons and holes at the opposite parts, which is beneficial for the separation of hydrogen and oxygen during the photocatalysis water splitting process.

4.
Horm Metab Res ; 51(11): 729-734, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31683343

RESUMO

Contrasting data about the association between proliferative diabetic retinopathy (PDR) and vitamin D status remain unknown. First, a hospital-based cross-sectional study consisting of 889 diabetic retinopathy (DR) and non-DR (NDR) patients was admitted. Further the accumulated evidence was performed to explore the association and dose-response relationship. Our study indicated that the odd ratio for PDR in vitamin D deficiency (VDD) individuals was significantly increased (1.60, 95% CI 1.06-2.42), compared with NDR in vitamin D sufficiency individuals, adjusted by age, sex, diabetic duration, and HbA1c. Four studies plus our study with data on vitamin D levels in 4970 patients with PDR and NDR subjects are compared. Association between vitamin D deficiency and risk of PDR exists (OR=1.69, 95% CI 1.40-2.05; I2=0%, p=0.61). Association between a nonlinear trend for vitamin D decrease with risk of DR was significant (chi2=16.53, p=0.0003). No significant heterogeneity in identified studies was found (goodness of fit chi2=2.98, p=0.225). It is concluded that vitamin D deficiency is significantly associated with risk of proliferative diabetic retinopathy.

5.
Opt Express ; 27(21): 29770-29780, 2019 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-31684234

RESUMO

We report on experimental generation and evolution of circumstance-susceptible, narrow-bandwidth, h-shaped pulse in a thulium-doped fiber (TDF) laser. With typical mode-locking technique based on nonlinear amplifying loop mirror, a type of h-shaped pulse is generated in a net normal dispersion regime for the first time to our best knowledge. Different from pulses with similar profiles achieved in typical anomalous dispersion regime, the h-shaped pulse here exhibits extremely narrow spectral bandwidth and meanwhile becomes highly circumstance-susceptible. Not alike the well-preserved h-shaped profile with anomalous dispersion, here the h-shaped pulse can easily evolve into various other pulse patterns with circumstance variations, including peak-depressed profiles, burst-like emission, multiple h-shaped pulses, and even some highly complex temporal cases. Despite that, the h-shaped pulse broadens as the pump power increasing, being a typical pump-related characteristic dominated by the peak-power-clamping effect. Moreover, it is observed that the h-shaped pulse profile can be re-shaped by incorporating a piece of unpumped TDF into the cavity, i.e., introducing some reabsorption. Our results substantiate the experimental revelation of such a type of particular-profile pulse in the normal dispersion regime, demonstrating some new evolution features facilitated by the dispersion-relevant circumstance-susceptibility.

6.
Biol Pharm Bull ; 42(11): 1953-1956, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31685778

RESUMO

Novel bivalent twin-drug type hydantoin derivatives were evaluated in vitro using a human brain glioma cell line (U251) and a human carcinoma cell line (KB3-1). Among the 5-substituted hydantoin derivatives (1a-b and 2a-d) examined in this study, bivalent symmetrical 5-substituted hydantoin derivative 1b showed the highest anti-proliferative activity towards both U251 and KB3-1 cells. The values of anti-proliferative activity (IC50) of this hydantoin derivative against the two cell lines (U251 and KB3-1) were 0.46 and 5.21 µM, respectively. The anti-proliferative activity of all of the compounds except for compounds 2a and 2d against U251 cells was higher than that of cisplatin. Bivalent symmetrical compound 1b had a biphenylmethane linker in the molecule. All of the tested bivalent hydantoin derivatives showed higher activity against U251 cells than against KB3-1 cells. For twin-drug type hydantoin derivatives 2a-d, which have a linear methylene linker in the molecules, it was found that methylene linker length in these molecules have an effect on the anti-proliferative activity against U251 and KB3-1 cells.

7.
Int Urol Nephrol ; 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31686279

RESUMO

BACKGROUND: Parathyroid glands consist of chief cells and oxyphil cells. The parathyroid oxyphil cell proportion increased dramatically in patients with chronic kidney disease (CKD). The importance of the oxyphil cell in the treatment of secondary hyperparathyroidism (SHPT) has been enhanced in recent years. The aim of this study was to explore the possible relationship between parathyroid oxyphil cell proportion and clinical features of patients with CKD. METHODS: From December 2010 to December 2018, 78 consecutive patients with CKD 5 who underwent parathyroidectomy (PTX) were reviewed. None of the patients had used Cinacalcet. Patients were divided into two groups according to their parathyroid oxyphil cell proportion. The mean parathyroid oxyphil cell proportion of all the patients was obtained. Group 1 consisted of 37 patients, the parathyroid oxyphil cell proportion of whom was more than or equal to mean proportion. Group 2 consisted of 41 patients, the parathyroid oxyphil cell proportion of whom was less than mean proportion. The demographic and biochemical data of the two groups were compared. RESULTS: Preoperative parathyroid hormone (PTH) and total weight of removed parathyroid glands in the patients of group 1were less than those in group 2. There was no difference between the two groups in terms of preoperative calcium (Ca), phosphorus (P), alkaline phosphatase (ALP), age and dialysis years. CONCLUSION: The parathyroid oxyphil cell proportion was associated with preoperative PTH and total weight of removed parathyroid glands in the patients with CKD. Patients with more parathyroid oxyphil proportion had lower preoperative PTH level and smaller total weight of the removed parathyroid glands.

8.
Chem Commun (Camb) ; 55(91): 13638-13648, 2019 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-31675019

RESUMO

The judicious incorporation of a fluoroalkyl moiety often brings about beneficial effects on the properties of bioactive molecules. Consequently, efficient methods for selective fluoroalkylation are much sought after in drug discovery. Despite significant achievements in trifluoromethylation, selective mono- and difluoroalkylation is still undeveloped. Catalytic functionalization of fluorinated silyl enol ethers (FSEEs) emerges as a fruitful approach for the diversity-oriented synthesis of value-added α-mono or difluoroalkylated ketones. In this feature article, we detail our efforts in developing catalytic selective mono- and difluoroalkylation reactions using FSEEs. Specifically, we highlight our findings such as activating FSEEs by amines for catalytic enantioselective synthesis, taking advantage of the often observed high activity of FSEEs over the non-fluorinated analogues for reaction development, and the influence of C-FH-X interactions on reactivity and selectivity.

9.
Orthop Surg ; 2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31714030

RESUMO

OBJECTIVE: Lumbar spinal stenosis is a medical condition characterized by the narrowing of the spinal canal as a consequence of bone and soft tissue degeneration, including disc herniation, facet and ligamentum flavum hypertrophy, and osteophyte formation. The percutaneous transforaminal endoscopic discectomy (PTED) technique is one of the emerging surgical alternatives for treating central lumbar stenosis. The present study aims to describe the present techniques of PTED and foraminoplasty for central lumbar stenosis, and discuss the feasibility and advantages of this technique. METHODS: A total of 55 patients with an average age of 50 years were recruited in this study. They were operated on between August 2017 and June 2018 by a single surgeon for symptomatic lumbar stenosis using the PTED and foraminoplasty technique, along with a detailed description of the present technique. The retrospective analysis of 55 patients operated between August 2017 and June 2018 by a single surgeon for symptomatic lumbar stenosis using the PTED and foraminoplasty techniques, and the detailed description of the present technique were the focus of the present study. For all patients, the PTED and foraminoplasty procedure was performed under local anesthesia in the lateral position on a radiolucent table using C-arm fluoroscopy. The retrospective analysis evaluated the outcomes of symptoms through follow-up interviews at six weeks, six months, and one year after surgery. The analyzed parameters included surgery time, intraoperative blood loss, postoperative complications, visual analog scale (VAS) score, Japan Orthopedic Association (JOA) score, and the Oswestry Disability Index (ODI). The modified MacNab criteria were adopted. RESULTS: The average duration of symptoms was 15.6 weeks. The mean operative time was 161 minutes. The mean volume of intraoperative blood loss was 21 mL. The mean follow-up period was 14.6 months. The average preoperative VAS score for leg pain and low back pain was 6.8 and 5.5, respectively. The preoperative ODI and JOA score was 49.2 and 14.6, respectively. At the final follow-up, all 55 patients had an average VAS score of 1.1 for leg pain and 0.5 for low back pain. At the same time, the average ODI and JOA score was seven and 24.5, respectively. The statistical analysis showed that the VAS score, ODI value, and JOA score were significantly lower in all time-points at post-operation, when compared to those at pre-operation. For the modified MacNab criteria, the final outcome results were excellent in 39 patients (70.9%), good in nine patients (16.4%), fair in four patients (7.3%), and poor in two patients (3.6%), and the overall success rate was 89.1%. Two patients underwent a second operation during the follow-up period, and their symptoms were released after the reoperation. CONCLUSION: PTED and foraminoplasty technique showed promising outcomes in the treatment of central lumbar stenosis in a 1-year follow-up period. It suggested that PTED and foraminoplasty might be applied as a safe and effective therapeutic option for patients with lumbar stenosis.

10.
Medicine (Baltimore) ; 98(44): e17821, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31689868

RESUMO

Although many genetic variants related to anti-tuberculosis drug induced liver injury (ATDILI) have been identified, the prediction and personalized treatment of ATDILI have failed to achieve, indicating there remains an area for further exploration. This study aimed to explore the influence of single nucleotide polymorphisms (SNPs) in Bradykinin receptor B2 (BDKRB2), Teneurin transmembrane protein 2 (TENM2), transforming growth factor beta 2 (TGFB2), and solute carrier family 2 member 13 (SLC2A13) on the risk of ATDILI.The subjects comprised 746 Chinese tuberculosis (TB) patients. Custom-by-design 2x48-Plex SNPscanTM kit was employed to genotype 28 selected SNPs. The associations of SNPs with ATDILI risk and clinical phenotypes were analyzed according to the distributions of allelic and genotypic frequencies and different genetic models. The odds ratio (OR) with corresponding 95% confidence interval (CI) was calculated.Among subjects with successfully genotyped, 107 participants suffered from ATDILI during follow-up. In BDKRB2, patients with rs79280755 G allele or rs117806152 C allele were more vulnerable to ATDILI (PBonferronicorrection = .002 and .03, respectively). Rs79280755 increased the risk of ATDILI significantly whether in additive (OR = 3.218, 95% CI: 1.686-6.139, PBonferroni correction = .003) or dominant model (PBonferroni correction = .003), as well as rs117806152 (Additive model: PBonferroni correction = .05; dominant model: PBonferroni correction = .03). For TENM2, rs80003210 G allele contributed to the decreased risk of ATDILI (PBonferroni correction = .02), while rs2617972 A allele conferred susceptibility to ATDILI (PBonferroni correction = .01). Regarding rs2617972, significant findings were also observed in both additive (OR = 3.203, 95% CI: 1.487-6.896, PBonferroni correction = .02) and dominant model (PBonferroni correction = .02). Moreover, rs79280755 and rs117806152 in BDKRB2 significantly affected some laboratory indicators. However, no meaningful SNPs were observed in TGFB2 and SLC2A13.Our study revealed that both BDKRB2 and TENM2 genetic polymorphisms were interrogated in relation to ATDILI susceptibility and some laboratory indicators in the Western Chinese Han population, shedding a new light on exploring novel biomarkers and targets for ATDILI.


Assuntos
Antituberculosos/efeitos adversos , Sinalização do Cálcio/genética , Doença Hepática Induzida por Substâncias e Drogas/genética , Proteínas de Membrana/genética , Proteínas do Tecido Nervoso/genética , Receptor B2 da Bradicinina/genética , Adulto , Grupo com Ancestrais do Continente Asiático/genética , Feminino , Marcadores Genéticos , Predisposição Genética para Doença , Proteínas Facilitadoras de Transporte de Glucose/genética , Humanos , Masculino , Pessoa de Meia-Idade , Polimorfismo de Nucleotídeo Único , Estudos Prospectivos , Fator de Crescimento Transformador beta2/genética
11.
Medicine (Baltimore) ; 98(44): e17686, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31689791

RESUMO

RATIONALE: Ependymomas are neuroepithelial tumors that typically occur in the central nervous system. Ependymomas arising in the mediastinum are exceedingly rare, with only approximately 9 isolated cases reported in the literature to date. PATIENT CONCERNS: A 35-year-old woman was referred to our hospital with complaints of progressive back pain for 3 months. Physical examination revealed decreased breathing sounds and tenderness. Contrast-enhanced computed tomography showed a soft tissue mass with heterogeneous enhancement in the right posterior mediastinum. DIAGNOSES: The diagnosis of primary mediastinal ependymomas (PMEs) was confirmed by postoperative histopathologic examination. INTERVENTIONS AND OUTCOMES: The patient underwent surgical resection of the tumor and experienced local recurrence with neck metastasis 2 years postoperatively. She underwent reoperation for the recurrent tumors and received postoperative radiotherapy and adjuvant chemotherapy. Two years later, the patient is doing well, with no evidence of tumor progression or recurrence. LESSONS: Since PMEs are exceedingly rare, treatment options are limited. Surgical resection seems to be the mainstay of treatment. Further evidence-based studies are required to prove the benefit of radiotherapy and chemotherapy in the treatment of PMEs.

12.
Theranostics ; 9(25): 7961-7975, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31695809

RESUMO

Rationale: Aortic dissection (AD) is caused by functional disorder of cells in the aortic wall, which is largely attributed to vascular remodeling. Therapeutic strategies for AD remain limited due to our incomplete understanding of the role of endothelial cells (ECs) in AD pathogenesis. This study aimed to identify the regulatory role of miR-27a in AD and provide a mechanistic basis for a non-invasive treatment of AD. Methods: We harvested aortas from normal and AD patients to explore the expression of miR-27a. In vitro and in vivo assays were preformed to explore the biological effects of differential expression of miR-27a in ECs and its regulatory effect on AD. Results: MiR-27a was lower in intima of AD samples than in healthy individuals. Downregulation of miR-27a in EC was due to up-regulated expression of fas-associated protein with death domain (FADD) and the activation of apoptosis pathway, which led to apoptosis of ECs. Migration of vascular smooth muscle cells was promoted by EC after downregulation of miR-27a due to enhancement of growth/differentiation factor 8 (GDF8) and repression of matrix metalloproteinase-20 (MMP20) in the co-culture system supernatants. Increase in FADD and apoptosis of ECs to induce AD was shown using mouse models of AD in which miR-27a was stably knocked-down by antagomir. Up-regulation of miR-27a by agomir led to a protective effect on AD. Conclusion: Treatment with miR-27a activator that targets apoptosis of ECs strongly diminished occurrence of AD, providing a new strategy for this disease.

13.
Food Chem ; : 125253, 2019 Jul 25.
Artigo em Inglês | MEDLINE | ID: mdl-31753681

RESUMO

To ensure accurate and reliable measurement results, a whole liquid egg certified reference material (CRM) retaining the resemblance to real samples was first developed for improving measurement quality of enrofloxacin residue in eggs. The CRM raw material naturally containing enrofloxacin was obtained by sampling eggs from laying hens following oral administration of enrofloxacin. The homogenizing and bottling procedure was evaluated and applied on the bulk raw material to prepare the CRM candidate. For characterization of the CRM, liquid chromatography tandem isotope dilution mass spectrometry as a reliable reference method was studied and used for value assignment by all participating laboratories. The certified value was assigned to be 30.6 µg/kg with expanded uncertainty of 3.1 µg/kg (coverage factor k = 2, approximate 95% confidence interval) for enrofloxacin. In addition, homogeneity, long-term stability at -70 °C for one year and short-term stability at -18 °C, 4 °C and room temperature for nine days were assessed.

14.
Jpn J Clin Oncol ; 2019 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-31735973

RESUMO

BACKGROUND: This meta-analysis aimed to investigate the prognostic value of lymph node ratio in non-small-cell lung cancer. METHODS: We searched systematically for eligible studies in PubMed, Web of Science, Medline (via Ovid) and Cochrane library through 6 November 2018. The primary outcome was overall survival. Disease-free survival and cancer-specific survival were considered as secondary outcomes. Hazard ratio with corresponding 95% confidence interval were pooled. Quality assessment of included studies was conducted. Subgroup analyses were performed based on N descriptors, types of tumor resection, types of lymphadenectomy and study areas. Sensitivity analysis and evaluation of publication bias were also performed. RESULTS: Altogether, 20 cohorts enrolling 76 929 patients were included. Mean Newcastle-Ottawa Scale was 7.65 ± 0.59, indicating the studies' quality was high. The overall result showed non-small-cell lung cancer patients with lower lymph node ratio was associated with better overall survival (HR: 1.946; 95% CI: 1.746-2.169; P < 0.001), disease-free survival (HR: 2.058; 95% CI: 1.717-2.467; P < 0.001) and cancer-specific survival (HR: 2.149; 95% CI: 1.864-2.477; P < 0.001). Subgroup analysis prompted types of lymphadenectomy and the station of positive lymph node have an important effect on the prognosis. No significant discovery was found in sensitivity analysis. CONCLUSION: Patients with lower lymph node ratio was associated with better survival, indicating that lymph node ratio may be a promising prognostic predictor in non-small-cell lung cancer. The type of lymphadenectomy, an adequate examined number and the removed stations should be considered for more accurate prognosis assessment.

15.
Future Oncol ; 15(34): 3917-3934, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31729887

RESUMO

Aim: To elucidate the integrative combinational gene regulatory network landscape of hepatocellular carcinoma (HCC) molecular carcinogenesis from diverse background. Materials & methods: Modified gene regulatory network analysis was used to prioritize differentially regulated genes and links. Integrative comparisons using bioinformatics methods were applied to identify potential critical molecules and pathways in HCC with different backgrounds. Results: E2F1 with its surrounding regulatory links were identified to play different key roles in the HCC risk factor dysregulation mechanisms. Hsa-mir-19a was identified as showed different effects in the three HCC differential regulation networks, and showed vital regulatory role in HBV-related HCC. Conclusion: We describe in detail the regulatory networks involved in HCC with different backgrounds. E2F1 may serve as a universal target for HCC treatment.

16.
Chem Commun (Camb) ; 55(95): 14303-14306, 2019 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-31713553

RESUMO

Herein, we report that 1,3-diphenylguanidine (DPG) could be utilized for the carboxylative cyclization of homopropargyl amines with CO2 under ambient temperature and pressure, in combination with AgSbF6, which enabled the synthesis of both chiral and achiral 2-oxazinones efficiently. A mechanistic study revealed that the multi-functionality of DPG is critical to the success of the reaction.

17.
Cancer Genet ; 240: 33-39, 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31726270

RESUMO

Circular RNAs (circRNAs), resulting from the non-canonical splicing of linear pre-mRNAs, have permanently altered our perspectives toward cancer recently, especially in carcinogenesis and cancer progression. However, the roles of circRNAs in esophageal squamous cell carcinoma (ESCC) remain largely unknown. In the current study, circRNAs expression profiles are screened in ESCC, using plasma samples from 10 ESCC patients, including different TNM stages and 5 normal controls. Characteristics of circRNAs including length, types, and the possibility of binding to proteins are analyzed. Candidate tumor-related circRNAs are then quantitated in ESCC tissues, plasmas and cell lines. ESCC tissues can secret circRNAs into plasma and patients with high plasma circ-SLC7A5 are associated with high TNM stage, tending to have shorter overall survival than those with high levels. In addition, the biological characteristics of circ-SLC7A5 including location, miRNAs binding, m6A modification were analyzed. Our study reveals a novel prognosis biomarker of circ-SLC7A5, providing a preliminary landscape of circRNA expression for detection of ESCC.

18.
Sci Total Environ ; 701: 134799, 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31726416

RESUMO

Road accessibility clearly reflects the spatial heterogeneity of urbanization. This study therefore adopted accessibility gradient to analyze the effects of urbanization on Cadmium (Cd) accumulation in agricultural soils. In total, 212 soil samples were collected along the accessibility gradient from agricultural soils in the Guangzhou-Foshan metropolitan region. Cd concentration showed a clearly decreasing pattern in agricultural soils with a decrease in accessibility level. The decreasing patterns varied in different accessibility ranges. The urban-rural ecotone (accessibility range 10-15) was the region with the most drastic changes in Cd accumulation. The influencing factors of Cd accumulation in agricultural soils mainly include industrial pollutants, agriculture chemicals, mining activities, domestic wastes, and soil properties. The importance of these factors varies across different accessibility ranges. Our findings imply that the characteristic variation of Cd accumulation with the road accessibility gradient must be considered in the formulation of targeted policies for controlling Cd contamination in agricultural soils.

19.
J Hazard Mater ; : 121588, 2019 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-31744728

RESUMO

Further understanding absorption uranium mechanism of the regenerational biosorbent is very interesting in application of the biosorbent. The regeneration adsorbent of Saccharomyces cerevisiae biomass was made by hydrochloric acid. Using it to absorb uranium at low constant pH(2.50), accompanied with proton releasing the ratio almost 1:2 which is to be analyzed in this paper. The type and amount of functional groups in the biomass such as carboxyl, amino, phosphoryl were determined by Potentiometric titrations and FTIR analysis. Chemical modification showed that the contribution of functional groups to uranium adsorption was carboxyl, phosphoryl and amino in turn. Analysis of SEM-EDX and staining microscopy showed that uranium on the surface of cells did not exist in the form of precipitation at lower pH 2.98, but at higher pH 4.52. The effects of phosphorus release and pH on uranium species was analyzed by MINTEQ software 3.0. Based on the above boundary conditions of the model construction, a multi-site of functional groups model equation of ion exchange absorption mechanism was built in which the final uranium ion concentration and pH as functions. It could well describe the exchange equilibrium of proton with uranium ion at pH2.50 to pH4.00.

20.
Opt Express ; 27(19): 26471-26482, 2019 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-31674528

RESUMO

Healthcare and biosensing have attracted wide attention worldwide, with the development of chip integration technology in recent decades. In terms of compact sensor design with high performance and high accuracy, photonic crystal structures based on Fano resonance offer superior solutions. Here, we design a photonic crystal structure for sensing applications by proposing modeling for a three-cavity-coupling system and derive the transmission expression based on temporal coupled-mode theory (TCMT). The correlations between the structural parameters and the transmission are discussed. Ultimately, the geometry, composed of an air mode cavity, a dielectric mode cavity and a cavity of wide linewidth, is proved to be feasible for simultaneous sensing of refractive index (RI) and temperature (T). For the air mode cavity, the RI and T sensitivities are 523 nm/RIU and 2.5 pm/K, respectively. For the dielectric mode cavity, the RI and T sensitivities are 145 nm/RIU and 60.0 pm/K, respectively. The total footprint of the geometry is only 14 × 2.6 (length × width) µm2. Moreover, the deviation ratios of the proposed sensor are approximately 0.6% and 0.4% for RI and T, respectively. Compared with the researches lately published, the sensor exhibits compact footprint and high accuracy. Therefore, we believe the proposed sensor will contribute to the future compact lab-on-chip detection system design.

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