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1.
Front Oncol ; 13: 1077961, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36950556

RESUMO

Background: The number of publications on acupuncture for cancer pain is increasing rapidly with an upward tendency. Considering that no bibliometric articles related to this topic have been published yet. It is necessary to evaluate the global scientific output of research in this field, and shed light on the direction of clinical cancer pain management in the future. Methods: Research publications regarding acupuncture on cancer pain from inception to 2022 were downloaded from the Web of Science Core Collection. Bibliometric analyses were performed using CiteSpace software, the bibliometrix R package, and VOSviewer software. Network maps were generated to assess the collaborations between different countries, institutions, authors, and keywords. And clusters map was generated to evaluate reference. Results: A total of 790 articles related to acupuncture therapy for cancer pain were identified. We observe that the number of publications is gradually increasing over time. China and the United States were the main contributors. Mem Sloan Kettering Canc Ctr (38 papers) and Beijing Univ Chinese Med (28 papers) contributed the most publications, becoming the leading contributors in this field. Although J Clin Oncol (28 articles) ranked ninth in terms of publication volume, it was the journal with the most citations and the highest number of IF (50.717) and H-index (494) at the same time. MAO J from Mem Sloan Kettering Canc Ctr was the most prolific author (23 articles). The main hot topics included matters related to acupuncture (239 times), pain (199 times), management (139 times), quality of life (107 times), electroacupuncture (100 times), and breast cancer (82 times). Conclusion: Our bibliometric analysis provides a comprehensive overview of the development of acupuncture for cancer pain, enabling relevant authors and research teams to identify the current research status in this field. At the same time, acupuncture for breast cancer (BC) pain, aromatase inhibitor-induced arthralgia (AIA), and chemotherapy-induced peripheral neuropathy (CIPN) may soon become prospective focus.

2.
J Immunother Cancer ; 11(2)2023 02.
Artigo em Inglês | MEDLINE | ID: mdl-36822671

RESUMO

BACKGROUND: Ovarian cancer is the deadliest type of malignant gynecological tumor. Polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs) are involved ovarian cancer and are closely related to adverse outcomes. However, the immunosuppressive mechanism of PMN-MDSCs remains elusive. METHODS: The types and numbers of ANKRD22-expressing cells were investigated by bioinformatics analysis and immunohistochemical staining. Ankrd22-/- C57BL/6 mice were constructed with CRISPR-Cas9 technology. Mouse PMN-MDSCs were obtained from bone marrow (BM)-derived CD11b+Ly6G+Ly6Clow cells sorted by fluorescence-activated cell sorting with treatment of GM-CSF and IL-6, and the immunosuppressive activity of PMN-MDSCs was evaluated by flow cytometry (FCM) and ELISA. The expression level of CCR2 and the exogenous glucose uptake capacity were determined by FCM. RT-qPCR was used to detect ANKRD22 expression in CD11b+HLA-DR-CD14-CD15+ cells from human ovarian cancer tissues, and the correlations of ANKRD22 expression with the clinical characteristics and prognosis of patients were evaluated by the χ2 test. RESULTS: We identified a novel protein involved in regulating the immunosuppressive ability of PMN-MDSCs, ANKRD22. Ankrd22 expression was high in mouse CD11b+Ly6G+Ly6Clow cells and could be significantly downregulated after exposure to a simulated microenvironmental stimulus. Knockout of Ankrd22 increased the expression level of CCR2 of CD11b+Ly6G+Ly6Clow cells and the immunosuppressive activity of PMN-MDSCs. BM-derived CD11b+Ly6G+Ly6Clow cells of Ankrd22-/- mice significantly promoted the proliferation of ovarian cancer cells in tumor xenograft mouse models. Mechanistically, RNA sequencing showed that Wdfy1 expression was obviously increased in Ankrd22-knockout BM-derived CD11b+Ly6G+ Ly6Clow cells and that ectopic expression of Wdfy1 increased the levels of Arg1, Inos, Ido and Pdl1 in Ankrd22+/+ PMN-MDSCs derived from BM-derived CD11b+Ly6G+Ly6Clow cells. Surprisingly, an ANKRD22-activating candidate small-molecule compound attenuated the immunosuppressive activity of Ankrd22+/+ PMN-MDSCs. Finally, we found that low ANKRD22 levels in CD11b+HLA-DR-CD14-CD15+ cells derived from primary ovarian tissues were associated with a more advanced International Federation of Gynecology and Obstetrics stage, a higher recurrence rate, and a higher neutrophil-to-lymphocyte ratio. CONCLUSIONS: These results suggest that ANKRD22 is a potential novel target for reversing the immunosuppressive effects of PMN-MDSCs.


Assuntos
Células Supressoras Mieloides , Neoplasias Ovarianas , Humanos , Camundongos , Feminino , Animais , Camundongos Knockout , Neoplasias Ovarianas/tratamento farmacológico , Neoplasias Ovarianas/metabolismo , Camundongos Endogâmicos C57BL , Antígenos HLA-DR , Imunossupressores
3.
RSC Adv ; 13(9): 5674-5686, 2023 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-36798748

RESUMO

As a decoration method, coupling a photocatalyst with semiconductor quantum dots has been proven to be an efficient strategy for enhanced photocatalytic performance. Herein, a novel BiOCl nanosheet decorated with Bi2O3 quantum dots (QDs) was first synthesized by a facile one-step in situ chemical deposition method at room temperature. The as-prepared materials were characterized by multiple means of analysis. The Bi2O3QDs with an average diameter of about 8.0 nm were uniformly embedded on the surface of BiOCl nanosheets. The obtained Bi2O3QDs/BiOCl exhibited significantly enhanced photocatalytic performance on the degradation of the rhodamine B and ciprofloxacin, which could be attributed to the band alignment, the photosensitization effect and the strong coupling between Bi2O3 and BiOCl. In addition, the dye photosensitization effect was demonstrated by the monochromatic photodegradation experiments. The radical trapping experiments and the ESR testing demonstrated the type II charge transfer route of the heterojunction. Finally, a reasonable photocatalytic mechanism based on the relative band positions was discussed to illustrate the photoreaction process. These findings provide a good choice for the design and potential application of BiOCl-based photocatalysts in water remediation.

4.
Animals (Basel) ; 13(2)2023 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-36670779

RESUMO

There are considerable challenges involved in studying the behavior of subterranean rodents owing to the underground nature of their ecotope. Seismic communication plays a crucial role in the behavior of subterranean rodents, particularly solitary ones. The plateau zokor (Eospalax baileyi), a solitary subterranean rodent species endemic to the Qinghai−Tibet Plateau, will usually occupy empty neighboring tunnels in order to extend their territory. Little is known, however, about the process of territorial occupation or the function of animal communication when occupation is taking place. Based on previous studies of subterranean rodent communication, we hypothesized that plateau zokors use seismic signals to detect neighboring tunnels and then occupy them when it was found their neighbors were absent. To test this, we placed artificial tunnels close to active original zokor tunnels to simulate the availability of an empty neighboring tunnel, and then the seismic signals when a zokor chose to occupy the empty artificial tunnel were recorded. The results showed that the frequency of zokors occupying artificial empty tunnels within 48 h was 7/8, In all of these instances, the zokors generated seismic signals before and after occupation of the empty artificial tunnel. The number of seismic signals generated by the zokors increased significantly (p = 0.024) when they detected and occupied the artificial tunnels, compared to those generated in their original tunnels without the presence of an artificial tunnel alongside. Inside the original tunnels, the inter-pulse time interval of the seismic signals was significantly higher (p < 0.001), the peak frequency of these signals was significantly higher (p < 0.01), and the energy of the signals was significantly lower (p = 0.006), compared with those when an artificial tunnel was positioned next to the original. The results of this study suggest that plateau zokors first generate seismic signals to detect empty neighboring tunnels and that they are empty. In the absence of neighbor plateau zokors, they occupy the empty tunnels to extend their own territory.

5.
J Adv Res ; 2023 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-36706988

RESUMO

INTRODUCTION: Myocardial fibrosis and cardiac dysfunction are the main characteristics of diabetic heart disease. However, the molecular mechanisms underlying diabetic myocardial fibrosis remain unclear. OBJECTIVES: This study aimed to investigate the heterogeneity of cardiac fibroblasts in diabetic mice and its possible mechanism in the development of diabetic myocardial fibrosis. METHODS: We established a diabetic mouse model by injecting mice with streptozotocin. The overall cell profiles in diabetic hearts were analyzed using single-cell RNA transcriptomic techniques. Cardiac function was evaluated by echocardiography. Cardiac fibrosis was assessed by Masson's trichrome and Sirius red staining. Protein expression was analyzed using Western blotting and immunofluorescence staining. RESULTS: A total of 11,585 cells were captured in control (Ctrl) and diabetic (DM) hearts. Twelve cell types were identified in this study. The number of fibroblasts was significantly higher in the DM hearts than in the Ctrl group. The fibroblasts were further re-clustered into nine subsets. Interestingly, cluster 4 fibroblasts were significantly increased in diabetic hearts compared with other fibroblast clusters. Lysyl oxidase (Lox) was highly expressed in DM fibroblasts (especially in cluster 4). Beta-aminopropionitrile, a Lox inhibitor, inhibited collagen expression and alleviated cardiac dysfunction in the diabetic group. Lysyl oxidase inhibition also reduced high glucose-induced collagen protein upregulation in primary fibroblasts. Moreover, a TGF-ß receptor inhibitor not only prevented an increase in Lox and Col I but also inhibited the phosphorylation of Smad2/3 in fibroblasts. CONCLUSIONS: This study revealed the heterogeneity of cardiac fibroblasts in diabetic mice for the first time. Fibroblasts with high expression of Lox (cluster 4 fibroblasts) were identified to play a crucial role in fibrosis in diabetic heart disease. The findings of this study may provide a possible therapeutic target for interstitial fibrosis.

6.
Medicine (Baltimore) ; 102(4): e32783, 2023 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-36705381

RESUMO

To explore factors related to local injection of Lauromacrogol combined with curettage in the treatment of cesarean scar pregnancy. A total of 24 successful and 8 unsuccessful cases were included. The age, gravidity, parity, times of cesarean section, interval from the last cesarean section, preoperative human chorionic gonadotropin (HCG), HCG on the first day after operation, decreasing rate of HCG on the first day after operation, average diameter of gestational sac, and preoperative vaginal bleeding days were analyzed. There were no significant differences of age, gravidity, parity, previous cesarean section times between groups. The differences of preoperative HCG, HCG on the first day after operation, the decreasing rate of HCG, gestational sac diameter, preoperative vaginal bleeding days were statistically significant between groups. The interval from the last cesarean section and the decreasing rate of HCG were protective factors, while the mean diameter of gestational sac and period of vaginal bleeding before operation were risk factors for the success of the treatment. The mean diameter of gestational sac owned the best predictive value.


Assuntos
Cesárea , Cicatriz , Curetagem , Polidocanol , Gravidez Ectópica , Soluções Esclerosantes , Feminino , Humanos , Gravidez , Cesárea/efeitos adversos , Gonadotropina Coriônica , Cicatriz/tratamento farmacológico , Cicatriz/etiologia , Cicatriz/cirurgia , Paridade , Polidocanol/administração & dosagem , Polidocanol/uso terapêutico , Gravidez Ectópica/tratamento farmacológico , Gravidez Ectópica/etiologia , Gravidez Ectópica/cirurgia , Estudos Retrospectivos , Resultado do Tratamento , Hemorragia Uterina/etiologia , Soluções Esclerosantes/administração & dosagem , Soluções Esclerosantes/uso terapêutico , Injeções Intralesionais
7.
Viruses ; 15(1)2023 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-36680281

RESUMO

The newly identified porcine Kobuvirus (PKV) has raised concerns owing to its association with diarrheal symptom in pigs worldwide. The process involving the emergence and global spread of PKV remains largely unknown. Here, the origin, genetic diversity, and geographic distribution of PKV were determined based on the available PKV sequence information. PKV might be derived from the rabbit Kobuvirus and sheep were an important intermediate host. The most recent ancestor of PKV could be traced back to 1975. Two major clades are identified, PKVa and PKVb, and recombination events increase PKV genetic diversity. Cross-species transmission of PKV might be linked to interspecies conserved amino acids at 13-17 and 25-40 residue motifs of Kobuvirus VP1 proteins. Phylogeographic analysis showed that Spain was the most likely location of PKV origin, which then spread to pig-rearing countries in Asia, Africa, and Europe. Within China, the Hubei province was identified as a primary hub of PKV, transmitting to the east, southwest, and northeast regions of the country. Taken together, our findings have important implications for understanding the evolutionary origin, genetic recombination, and geographic distribution of PKV thereby facilitating the design of preventive and containment measures to combat PKV infection.


Assuntos
Kobuvirus , Infecções por Picornaviridae , Doenças dos Suínos , Suínos , Animais , Coelhos , Ovinos , Filogeografia , Kobuvirus/genética , Filogenia , Infecções por Picornaviridae/epidemiologia , Infecções por Picornaviridae/veterinária , Infecções por Picornaviridae/diagnóstico , Recombinação Genética
8.
Int Heart J ; 2023 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-36682769

RESUMO

An imbalance between inflammation-resolving lipid mediators and proinflammatory leukotrienes with the instability of atherosclerotic plaques in experimental models has been reported. However, the contribution of the balance of Resolvin D1 (RvD1) to Leukotriene B4 (LTB4) in predicting acute coronary syndrome (ACS) remains unknown. This study investigated the association of RvD1-to-LTB4 ratio with ACS.Eighty-one patients with ACS and 90 stable coronary artery disease (SCAD) patients were included in this study. Plasma RvD1 and LTB4 levels were measured with commercial kits.Patients with ACS had higher LTB4 levels, lower RvD1 levels, and a lower RvD1-to-LTB4 ratio than patients with SCAD. History of diabetes mellitus, elevated Troponin I, LTB4, and decreased RvD1-to-LTB4 ratio (odds ratio [OR]: 1.025; 95% confidence interval [CI]: 1.014-1.040; P < 0.001) were independently correlated with ACS. Receiver operating characteristic curve analysis demonstrated that RvD1-to-LTB4 ratio was a potential biomarker for the risk of ACS.A circulating proinflammatory lipid profile, characterized by a low RvD1-to-LTB4 ratio may be associated with ACS in patients with ischemic heart disease.

9.
Microbiol Spectr ; 11(1): e0341322, 2023 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-36537793

RESUMO

Avian metapneumovirus subgroup C (aMPV/C) is an important pathogen that causes upper respiratory symptoms and egg production decline in turkeys and chickens. aMPV/C infection leads to inhibition of the host antiviral immune response. However, our understanding of the molecular mechanisms underlying host immune response antagonized by aMPV/C infection is limited. In this study, we demonstrated that the aMPV/C phosphoprotein (P) inhibits the IFN antiviral signaling pathway triggered by melanoma differentiation gene 5 (MDA5) and reduces interferon ß (IFN-ß) production and IFN-stimulated genes (ISGs) by targeting IFN regulatory factor 7 (IRF7) but not nuclear factor κB (NF-κB) in DF-1 cells. Moreover, we found that aMPV/C P protein only blocks the nuclear translocation of IRF3 by interacting with IRF3 in HEK-293T cells, instead of affecting IRF3 phosphorylation and inducing IRF3 degradation, which suppresses IRF3 signaling activation and results in a decrease in IFN-ß production. Collectively, these results reveal a novel mechanism by which aMPV/C infection disrupts IFN-ß production in the host. IMPORTANCE The innate immune response is the first defense line of host cells and organisms against viral infections. When RNA viruses infect cells, viral RNA induces activation of retinoic acid-induced gene I and melanoma differentiation gene 5, which initiates downstream molecules and finally produces type I interferon (IFN-I) to regulate antiviral immune responses. The mechanism for avian metapneumovirus (aMPV) modulating IFN-I production to benefit its replication remains unknown. Here, we demonstrate that phosphoprotein of aMPV subgroup C (aMPV/C) selectively inhibits the nuclear translocation of interferon regulatory 3 (IRF3), instead of affecting the expression and phosphorylation of IRF3, which finally downregulates IFN-I production. This study showed a novel mechanism for aMPV/C infection antagonizing the host IFN response.


Assuntos
Fator Regulador 3 de Interferon , Interferon Tipo I , Metapneumovirus , Fosfoproteínas , Animais , Galinhas , Interações Hospedeiro-Patógeno , Fator Regulador 3 de Interferon/genética , Interferon Tipo I/metabolismo , Interferon beta , Metapneumovirus/metabolismo , Metapneumovirus/patogenicidade , Fosfoproteínas/genética , Fosfoproteínas/metabolismo , Proteínas Virais/metabolismo
10.
Purinergic Signal ; 19(1): 273-281, 2023 03.
Artigo em Inglês | MEDLINE | ID: mdl-36515790

RESUMO

Obesity is a public-health challenge resulting from an imbalance between energy expenditure and calorie intake. This health problem exacerbates a variety of metabolic complications worldwide. Adipose tissue is an essential regulator of energy homeostasis, and the functions within it are regulated by purinergic receptors. A1R, P2X7R, and P2YR mainly mediate energy homeostasis primarily through regulating energy storage and adipokines secretion in white adipose tissue (WAT). P2X5R is a novel-specific cell surface marker in brown/beige adipocytes. A2R is a promising therapeutic target for stimulating energy expenditure in brown adipose tissue (BAT) and also mediating WAT browning. Based on these features, purinergic receptors may be an appropriate target in treating obesity. In this review, the role of purinergic receptors in different types of adipose tissue is summarized. An improved understanding of purinergic receptor functions in adipose tissue may lead to more effective treatment interventions for obesity and its related metabolic disorders.


Assuntos
Tecido Adiposo Marrom , Tecido Adiposo Branco , Humanos , Tecido Adiposo Marrom/metabolismo , Tecido Adiposo Branco/metabolismo , Obesidade/metabolismo , Metabolismo Energético , Receptores Purinérgicos/metabolismo
11.
J Hazard Mater ; 446: 130622, 2023 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-36580776

RESUMO

The Xikuangshan (XKS) mine, the world's largest antimony (Sb) mine, was chosen for a detailed Sb isotopic signature study owing to its historical Sb contamination of water systems. Hydrochemical data, in particularδ123Sb values, were analyzed to identify the Sb source and predominant geochemical processes that affect Sb mobilization in different waters. The δ123Sb values of waters from the XKS Sb mine range from - 0.20‰ to + 0.73‰. In particular, the δ123Sb values of the main Feishuiyan stream do not significantly vary (+0.19‰-+0.24‰), while those of groundwater in different aquifers (-0.08‰ to +0.73‰) and mine water in different adits (-0.20‰ to +0.37‰) vary over a wide range. The relationships between δ123Sb values and Sb concentrations indicate that a simple dilution of Sb and a weak Sb adsorption onto Fe/Mn suspended particles and sediments in the Feishuiyan stream may occur, oxidative weathering and leaching infiltration of Sb-containing waste rocks and slags may cause variations in the δ123Sb values in groundwater, and Sb mobilization in the mine water is influenced by a combination of processes (oxidative dissolution, adsorption of Fe/Mn (hydr)oxides, and mixing). A conceptual hydrogeochemical model was summarized to elucidate the Sb source and mobilization in water systems from the XKS Sb mine.

12.
Anim Nutr ; 12: 77-86, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36514373

RESUMO

Yaks (Bos grunniens), indigenous to the harsh Qinghai-Tibetan Plateau, are well adapted to the severe conditions, and graze natural pasture without supplements all year round. Qaidam cattle (Bos taurus), introduced to the Qinghai-Tibetan Plateau 1,700 years ago, are raised at a lower altitude than yaks, provided with shelter at night and offered supplements in winter. Based on their different backgrounds, we hypothesized that yaks have lower energy requirements for maintenance than cattle. To test this hypothesis, we measured average daily gain (ADG), apparent digestibilities, energy balance, rumen fermentation parameters, and serum metabolites in growing yaks and cattle offered diets differing in metabolizable energy (ME) levels (6.62, 8.02, 9.42 and 10.80 MJ/kg), but with the same crude protein concentration. Six castrated yaks (155 ± 5.8 kg) and 6 castrated Qaidam cattle (154 ± 8.0 kg), all 2.5 years old, were used in 2 concurrent 4 × 4 Latin square designs. Neutral and acid detergent fiber digestibilities were greater (P < 0.05) in yaks than in cattle, and decreased linearly (P < 0.05) with increasing dietary energy level; whereas, digestibilities of dry matter, organic matter, crude protein and ether extract increased (P < 0.05) linearly with increasing energy level. The ADG was greater (P < 0.001) in yaks than in cattle, and increased (P < 0.05) linearly with increasing energy levels. From the regressions of ADG on ME intake, the estimated ME requirement for maintenance was lower (P < 0.05) in yaks than in cattle (0.43 vs. 0.57 MJ/kg BW0.75). The ratios of digestible energy (DE):gross energy and ME:DE were higher (P < 0.05) in yaks than in cattle, and increased (P < 0.05) linearly with increasing dietary energy level. Ruminal pH decreased (P < 0.05), whereas concentrations of total volatile fatty acids (VFAs) and ammonia increased (P < 0.01) with increasing dietary energy level, and all were greater (P < 0.05) in yaks than in cattle. Concentrations of ruminal acetate and iso-VFAs were greater (P < 0.05), whereas propionate was lower (P < 0.05) in yaks than in cattle; acetate decreased (P < 0.001), whereas butyrate and propionate increased (P < 0.001) linearly with increasing dietary energy level. Serum concentrations of ß-hydroxybutyrate were lower (interaction, P < 0.001) in yaks than in cattle fed diets of 9.42 and 10.80 MJ/kg, whereas non-esterified fatty acids were greater (interaction, P < 0.01) in yaks than in cattle fed diets of 6.62 and 8.02 MJ/kg. Concentrations of serum leptin and growth hormone were greater in yaks than in cattle and serum insulin and growth hormone increased (P < 0.01) linearly with increasing dietary energy level. Our hypothesis that yaks have lower energy requirements for maintenance than cattle was supported. This lower requirement confers an advantage to yaks over Qaidam cattle in consuming low energy diets during the long winter on the Qinghai-Tibetan Plateau.

13.
J Virol ; : e0144622, 2022 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-36472440

RESUMO

Seneca Valley virus (SVV), a new pathogen resulting in porcine vesicular disease, is prevalent in pig herds worldwide. Although an understanding of SVV biology pathogenesis is crucial for preventing and controlling this disease, the molecular mechanisms for the entry and post-internalization of SVV, which represent crucial steps in viral infection, are not well characterized. In this study, specific inhibitors, Western blotting, and immunofluorescence detection revealed that SVV entry into PK-15 cells depends on low-pH conditions and dynamin. Furthermore, results showed that caveolae-mediated endocytosis (CavME) contributes crucially to the internalization of SVV, as evidenced by cholesterol depletion, downregulation of caveolin-1 expression by small interfering RNA knockdown, and overexpression of a caveolin-1 dominant negative (caveolin-1-DN) in SVV-infected PK-15 cells. However, SVV entry into PK-15 cells did not depend on clathrin-mediated endocytosis (CME). Furthermore, treatment with specific inhibitors demonstrated that SVV entry into PK-15 cells via macropinocytosis depended on the Na+/H+ exchanger (NHE), p21-activated kinase 1 (Pak1), and actin rearrangement, but not phosphatidylinositol 3-kinase (PI3K). Electron microscopy showed that SVV particles or proteins were localized in CavME and macropinocytosis. Finally, knockdown of GTPase Rab5 and Rab7 by siRNA significantly inhibited SVV replication, as determined by measuring viral genome copy numbers, viral protein expression, and viral titers. In this study, our results demonstrated that SVV utilizes caveolae-mediated endocytosis and macropinocytosis to enter PK-15 cells, dependent on low pH, dynamin, Rab5, and Rab7. IMPORTANCE Entry of virus into cells represents the initiation of a successful infection. As an emerging pathogen of porcine vesicular disease, clarification of the process of SVV entry into cells enables us to better understand the viral life cycle and pathogenesis. In this study, patterns of SVV internalization and key factors required were explored. We demonstrated for the first time that SVV entry into PK-15 cells via caveolae-mediated endocytosis and macropinocytosis requires Rab5 and Rab7 and is independent of clathrin-mediated endocytosis, and that low-pH conditions and dynamin are involved in the process of SVV internalization. This information increases our understanding of the patterns in which all members of the family Picornaviridae enter host cells, and provides new insights for preventing and controlling SVV infection.

14.
Opt Express ; 30(26): 46911-46925, 2022 Dec 19.
Artigo em Inglês | MEDLINE | ID: mdl-36558631

RESUMO

The image information of distant objects shows a diffuse speckle pattern due to diffraction limit, non-uniform scattering, etc., which is difficult to achieve object discrimination. In this study, we have developed a staring spectral video imaging system mounted on a ground-based telescope observation platform to detect the high orbit space objects and gain their spectral images for six groups of GEO targets. The speckle remains basically the same characteristic as the projection structure of the object due to "the balloon inflation phenomenon of near parallel light during long-distance atmospheric transmission" under the premise of considering the bi-directional reflection distribution function (BRDF), Rayleigh scattering theory, and the memory effect. Based on this phenomenon, a mathematical model of remote target scattering spectrum imaging is established where the speckle can be treated as both a global speckle and speckle combination of texture blocks caused by various components of the target. The radial basis function (RBF) neural network is separately used to invert the global speckle and the speckle combination of the texture blocks on account of the typical target material database. The results show that the target materials are of relatively fewer kinds in the global inversion with only including gallium arsenide panel (GaAs) and carbon fiber (CF), for which the highest goodness of curve fitting is only 77.97. An improved algorithm makes their goodness of fit reach 90.29 and 93.33, respectively, in view of one conjecture that the target surface contains unknown materials. The spectral inversion result of the texture blocks shows that the types of materials in each target texture block increase significantly, and that the area ratio of different materials inverted in the block is different from each other. It is further confirmed that the speckle image contains the overall projection structure of distant target and the spectral image projection of each component is relatively fixed, which is the result of the comprehensive action of various mechanisms of ultra-long-haul atmospheric transmission and optical system focusing imaging after BRDF spectral scattering. The spectral image fine inversion is expected to restore the clear structure of the target. This discovery provides important support for the remote imaging and identification of distant and ultra-diffractive targets.

15.
Int J Biol Macromol ; 223(Pt A): 837-850, 2022 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-36343838

RESUMO

A new multifunctional film with active and intelligent effects was developed by incorporating curcumin-clove oil emulsion into natural materials. The basic properties, functional characteristics, and pH/NH3-sensitivity of films were investigated, and then these films were applied to extend shelf-life and monitor freshness of meat. Curcumin solution and emulsion illustrated significant color variations at different pH values. The incorporation of emulsion improved the UV-vis barrier and water resistance properties of films, which blocked most of UV-light and its water contact angle reached 100.03°. Meanwhile, the films had stronger mechanical strength and higher thermal stability, with elongation at break reaching 79.18 % and the maximum degradation temperature rising to 316 °C. Moreover, emulsion made films have a slow-release effect on clove oil, which not only enhanced the antioxidant property but also significantly improved their antibacterial activity. Additionally, the multifunctional films presented a significant color response to acidic/alkaline environments over a short time interval and could be easily identified by naked eyes. Finally, the films effectively extended the shelf-life of fresh meat by 3 days at 4 °C and visually monitored freshness through color changes in real-time. This knowledge provides insights and ideas for the development of novel food packaging with both active and intelligent functions.

16.
RSC Adv ; 12(51): 33449-33458, 2022 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-36425167

RESUMO

Two-dimensional vertically aligned Bi2O3 nanosheets over carbon paper (CP) were prepared via an in situ growth approach. Bi2O3/CP exhibits a robust photocatalytic activity, as well as renewability and flexibility. With Rhodamine B and 2,4-dichlorophenol used as target pollutants, the rate constant of Bi2O3/CP was 3.72 × 10-3 min-1 and 6.93 × 10-3 min-1 under visible-light irradiation for 2 h, respectively. The improved activity was attributed to the synergistic effects of the hierarchical structure of Bi2O3 and the conductive substrate, CP; the former provided efficient catalytic sites for the pollutants and absorbed more of the light scattered among the nanosheets, while the latter is beneficial to the photogenerated electron transfer.

17.
Animals (Basel) ; 12(22)2022 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-36428439

RESUMO

The fruit of Nitraria tangutorum (FNT) is reputed to possess medicinal properties; however, its effect on sheep (Ovis aries) is unknown. The aim of this study was to fill this gap. In a 3 × 3 Latin square design, six 12-month-old rumen-fistulated Hu rams (56.2 ± 8.26 kg; mean ± SD) were penned individually and offered one of three levels of FNT, namely, 0 g/d (control; CON), 16 g/d (N16), and 48 g/d (N48). The concentration of serum immunoglobulin G increased linearly (p = 0.03) with an increasing intake of FNT. The serum concentration of ß-hydroxybutyrate in the N48 group was lower than in the CON group (p = 0.01) and decreased linearly with increasing FNT (p = 0.001). The concentration of serum lactate dehydrogenase tended to decrease (p = 0.07) linearly with an increase in FNT intake, while the concentration of glucose did not differ among groups (p = 0.14) but displayed a quadratic curve with an increase in FNT (p = 0.05). The rumen concentration of lipase decreased linearly with increasing FNT (p = 0.04). The rumen fermentation variables were not affected by FNT. The FNT intake increased the abundance of beneficial ruminal bacteria, such as Lachnoclostridium, Rhodocyclaceae, and Candidatus Arthromitus. Prevotella, Rikenellaceae_RC9_gut_group, Ruminococcus, Olsenella, Lachnospiraceae_NK3A20_group, and Quinella were the dominant bacterial genera in all treatments. We conclude that FNT can improve immunity and increase the relative abundance of beneficial ruminal bacteria in sheep.

18.
Front Genet ; 13: 1017458, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36338953

RESUMO

Cold tolerance is an important trait for sheep raised at high altitudes. Muscle tissue, comprising 30-40% of the total body mass, produces heat during cold exposure. However, little is known about the genetic mechanisms of this tissue and its role in thermogenesis in lambs. We examined genes in skeletal muscle tissue in a cold-adapted sheep breed, Altay, and a cold-intolerant sheep breed, Hu, when exposed to low air temperature. Three ewe-lambs of each breed were maintained at -5°C and three ewe-lambs of each breed were maintained at 20°C. After cold exposure for 25 days, the longissimus dorsi of each lamb was collected, and transcriptome profiles were sequenced and analyzed. The results of RNA-seq showed that the average reads among the four groups were 11.0 Gbase. The genome mapping rate averaged 88.1% and the gene mapping rate averaged 82.5%. The analysis of differentially expressed genes (DEGs) indicated that the peroxisome proliferator-activated receptors (PPAR), cAMP, and calcium signaling pathways and muscle contraction in muscle tissue were linked to thermogenesis in cold-exposed lambs. Furthermore, PCK1 (phosphoenolpyruvate carboxykinase1) increased glyceroneogenesis in cold-exposed Altay lambs, and APOC3 (apolipoprotein C3), LPL (lipoprotein lipase), and FABP4 (fatty acid binding protein 4, adipocyte) were involved in the intake and transport of free fatty acids. In Hu sheep, cAMP biosynthesis from ATP hydrolysis was regulated by ADCY10 (adenylate cyclase) and ADORA2a (adenosine A2a receptor). Skeletal muscle contraction was regulated by MYL2 (myosin light chain 2). In conclusion, cold exposure altered the expression level of genes involved in heat production in muscle tissue. Some potential mechanisms were revealed, including calcium ion transport in the calcium signaling pathway, fatty acid metabolism in the PPAR signaling pathway, and cAMP biosynthesis in the cAMP signaling pathway. This study implied that skeletal muscle plays an important role in thermoregulation in lambs.

19.
Comput Intell Neurosci ; 2022: 9002713, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36238671

RESUMO

With the development of modern Internet technology, the health assessment model based on computer technology has gradually become a research hotspot. In the process of studying the health level of residents, exercise status and diet nutrition are important factors affecting their health. Therefore, based on the idea of the genetic algorithm, this paper establishes a resident sports nutrition data monitoring system. In this system, the data feature selection method of the genetic algorithm, which simulates the process of biological evolution, is used in the key technology of real-time motion data processing of residents, and it is improved to interfere with the data cross process of the genetic algorithm, to seek the local optimal solution. Two different types of data sets and different data classifiers are selected to verify the system's performance. It is proved that compared with the traditional filter class feature selection method, this method can achieve more effective data feature recognition. In addition, some samples are selected to test the residents' sports nutrition data monitoring system, mainly through the analysis and quantification of the exercise process, eating habits, physique, and other data of the athletes; to evaluate the impact of their sports and eating habits on physical health; and to obtain the best sports guidance scheme, to guide the adjustment and improvement of their later sports and eating plans. Through the analysis of the existing residents' health status, the monitoring and management strategies of residents' health status are summarized, which provides a certain reference for the improvement of residents' physical health under the background of artificial intelligence.


Assuntos
Inteligência Artificial , Esportes , Comportamento Alimentar , Humanos , Estado Nutricional
20.
Appl Opt ; 61(25): 7349-7353, 2022 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-36256033

RESUMO

Frequency conversion imaging technology can provide an effective way for infrared detection against the limitations of conventional infrared detectors, such as expense and cooling requirements, but the converted luminescence intensity of frequency conversion materials limits the application of this technology. In this paper, a cascade material (CM) fusion method is proposed to improve the conversion luminous intensity and thus enhance the frequency conversion imaging effect at 1550 nm near infrared (NIR) excitation. First, we derived from the energy level transition mechanism of CM that the CM fusion method can achieve three excitations of substrate materials (SMs). It can improve the conversion luminescence intensity of SM in CM. Then, we experimentally prepared CM and SM films and simultaneously measured the frequency conversion imaging effect of the two films at 1550 nm NIR excitation. It was found that the weight ratio of doped material (DM) to SM affects the imaging enhancement of CM films. Therefore, we compared the imaging grayscale value intensity of CM films with different weight ratios under the same detection conditions. Finally, it was concluded that the best enhancement of frequency conversion imaging was achieved with a DM to SM weight ratio of 0.25 for this mechanism. The enhancement was about 3.11 times compared to SM films.

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