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1.
BMC Cancer ; 19(1): 988, 2019 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-31647032

RESUMO

BACKGROUND: Laparoscopic surgery, fast-track perioperative treatment and XELOX chemotherapy are effective strategies for shortening the duration of hospital stay for cancer patients. This trial aimed to clarify the safety and efficacy of the fast-track multidisciplinary treatment (FTMDT) model compared to conventional surgery combined with chemotherapy in Chinese colorectal cancer patients. METHODS: This trial was a prospective randomized controlled study with a 2 × 2 balanced factorial design and was conducted at six hospitals. Patients in group 1 (FTMDT) received fast-track perioperative treatment and XELOX adjuvant chemotherapy. Patients in group 2 (conventional treatment) received conventional perioperative treatment and mFOLFOX6 adjuvant chemotherapy. Subgroups 1a and 2a had laparoscopic surgery and subgroups 1b and 2b had open surgery. The primary endpoint was total length of hospital stay during treatment. RESULTS: A total of 374 patients were randomly assigned to the four subgroups, and 342 patients were finally analyzed, including 87 patients in subgroup 1a, 85 in subgroup 1b, 86 in subgroup 2a, and 84 in subgroup 2b. The total hospital stay of group 1 was shorter than that of group 2 [13 days, (IQR, 11-17 days) vs. 23.5 days (IQR, 15-42 days), P = 0.0001]. Compared to group 2, group 1 had lower surgical costs, fewer in-hospital complications and faster recovery (all P < 0.05). Subgroup 1a showed faster surgical recovery than that of subgroup 1b (all P < 0.05). There was no difference in 5-year overall survival between groups 1 and 2 [87.1% (95% CI, 80.7-91.5%) vs. 87.1% (95% CI, 80.8-91.4%), P = 0.7420]. CONCLUSIONS: The FTMDT model, which integrates laparoscopic surgery, fast-track treatment, and XELOX chemotherapy, was the superior model for enhancing the recovery of Chinese patients with colorectal cancer. TRIAL REGISTRATION: ClinicalTrials.gov: NCT01080547 , registered on March 4, 2010.

2.
Cancer Res ; 79(18): 4729-4743, 2019 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-31308046

RESUMO

Quiescent cancer stem cells (CSC) play important roles in tumorigenesis, relapse, and resistance to chemoradiotherapy. However, the determinants of CSC quiescence and how they sustain themselves to generate tumors and relapse beyond resistance to chemoradiotherapy remains unclear. Here, we found that SET domain-containing protein 4 (SETD4) epigenetically controls breast CSC (BCSC) quiescence by facilitating heterochromatin formation via H4K20me3 catalysis. H4K20me3 localized to the promoter regions and regulated the expression of a set of genes in quiescent BCSCs (qBCSC). SETD4-defined qBCSCs were resistant to chemoradiotherapy and promoted tumor relapse in a mouse model. Upon activation, a SETD4-defined qBCSC sustained itself in a quiescent state by asymmetric division and concurrently produced an active daughter cell that proliferated to produce a cancer cell population. Single-cell sequence analysis indicated that SETD4+ qBCSCs clustered together as a distinct cell type within the heterogeneous BCSC population. SETD4-defined quiescent CSCs were present in multiple cancer types including gastric, cervical, ovarian, liver, and lung cancers and were resistant to chemotherapy. SETD4-defined qBCSCs had a high tumorigenesis potential and correlated with malignancy and chemotherapy resistance in clinical breast cancer patients. Taken together, the results from our previous study and current study on six cancer types reveal an evolutionarily conserved mechanism of cellular quiescence epigenetically controlled by SETD4. Our findings provide insights into the mechanism of tumorigenesis and relapse promoted by SETD4-defined quiescent CSCs and have broad implications for clinical therapies. SIGNIFICANCE: These findings advance our knowledge on the epigenetic determinants of quiescence in cancer stem cell populations and pave the way for future pharmacologic developments aimed at targeting drug-resistant quiescent stem cells.

4.
Int J Cancer ; 145(6): 1517-1528, 2019 09 15.
Artigo em Inglês | MEDLINE | ID: mdl-30720863

RESUMO

To gain more information on the prevalence of germline mutations in BRCA1/2 and PALB2 genes in the Chinese population, and to explore the effects of the mutation status of these genes on clinical outcomes in patients with breast cancer, we performed a screening for BRCA1/2 and PALB2 mutations in a consecutive series of unselected breast cancer patients in the Chinese population. A total of 2,769 cases were enrolled between June 1993 and September 2017. All of the exons and exon-intron boundaries of the BRCA1/2 and PALB2 genes were screened with next-generation sequencing. Of the 2,769 breast cancer patients, BRCA1, BRCA2 and PALB2 mutations accounted for 2.7% (n = 74), 2.7% (n = 76), and 0.9% (n = 24), respectively. The BRCA1 gene had the highest mutation frequency in patients with triple-negative breast cancer (TNBC), which was 9.6% (n = 42), while the BRCA2 gene had the highest mutation frequency in patients with Luminal, which was 3.2% (n = 58). The disease-free survival (DFS) of BRCA1 mutation carriers was significantly lower than that of noncarriers (adjusted HR = 2.20, 95% CI = 1.15-4.18, p = 0.017). The mutation status of the PALB2 gene was significantly associated with the decline in overall survival (OS) (adjusted HR = 8.38, 95% CI = 2.19-32.11, p = 0.002). No significant difference was found between BRCA2 pathogenic mutation carriers and noncarriers. These results demonstrate that BRCA1 mutation status may be associated with a worse disease progression in patients with breast cancer, and women who harbored a PALB2 mutation might be at a higher risk of death due to breast cancer compared to noncarriers.

5.
Onco Targets Ther ; 12: 87-99, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30588033

RESUMO

Background: Tanshinol is an active constituent of Salvia miltiorrhiza and possess anti-inflammatory, antioxidant, and anti-bacterial activity. Herein, we explored the role of tanshinol on the growth and aggressiveness of hepatocellular carcinoma (HCC) cells in vitro and in vivo. Materials and methods: The proliferation of a panel of HCC cell lines was measured using MTT assay. The expressions of phosphatidylinositol 3 kinase (PI3K) and protein kinase B (AKT) were detected by immunofluorescence staining and immunohistochemical assay. The levels of Bcl-2 and Bax were determined using immunoblotting assay. The secretions of matrix metalloproteinase-2 (MMP-2) and MMP-9 were detected by ELISA. The migration and invasion abilities of HepG2 cell were determined using wound healing and Transwell invasion assays. The apoptosis of HepG2 cell induced by tanshinol was analyzed by Annexin V/propidium iodide staining. A xenograft model was constructed to investigate the inhibitory effect of tanshinol on HepG2 cell growth in vivo. To further investigate the role of tanshinol on the metastasis of HepG2 cell in vivo, an experimental metastasis assay was performed. Results: Tanshinol inhibited the growth and colony formation of HCC cell in vitro. Tanshinol also induced the apoptosis of HepG2 cell and inhibited the migration and invasion of HepG2 cell. In in vivo experiments, tanshinol suppressed the tumor growth and metastasis of HepG2 cell. Furthermore, the phosphorylation of PI3K and AKT was decreased by tanshinol in vitro and in vivo. Conclusion: Tanshinol exerts its anti-cancer effects via regulating the PI3K-AKT signaling pathway in HCC.

6.
Chemistry ; 24(68): 18106-18114, 2018 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-30230075

RESUMO

Designing core-shell electrode materials with desired components and architectures is a promising strategy for boosting electrochemical performance. Here, three-dimensional hierarchical ZnCo2 O4 @Ni(OH)2 core-shell nanosheet arrays have been successfully fabricated on a Ni foam substrate, in which the porous ZnCo2 O4 nanosheet "core" as the conductive scaffold was synthesized by a metal-organic framework (MOF)-templated method, and the ultrathin Ni(OH)2 nanoflakes "shell" with rich active sites were grafted on the ZnCo2 O4 nanosheet through a hydrothermal treatment. When directly used as a free-standing electrode for supercapacitor, these hierarchical ZnCo2 O4 @Ni(OH)2 core-shell nanosheet arrays exhibited a high capacitance of 3063.2 mF cm-2 (1021.1 F g-1 ) at the current density of 1 mA cm-2 . This electrode significantly outperformed individual Ni(OH)2 or ZnCo2 O4 nanosheet arrays, benefiting from the robust core-shell arrays on Ni foam with good electrical conductivity and abundant active sites, as well as the synergetic effect between MOF-derived porous ZnCo2 O4 "core" and the ultrathin Ni(OH)2 "shell". Moreover, the assembled ZnCo2 O4 @Ni(OH)2 //activated-carbon asymmetric supercapacitor displayed excellent energy and power densities (maximum of 40.0 Wh kg-1 and 8.02 kW kg-1 ) and superior cycling stability of 98.4 % retention with 91.2 % coulombic efficiency over 5 000 cycles at 10 A g-1 .

7.
Inorg Chem ; 57(11): 6202-6205, 2018 Jun 04.
Artigo em Inglês | MEDLINE | ID: mdl-29756772

RESUMO

A novel hierarchical nanoarray material based on a two-dimensional metal-organic framework (Ni-CAT) and a layered double hydroxide (NiCo-LDH) was fabricated on a nickel foam substrate. By taking advantage of the regular nanostructure and making full use of the high porosity and excellent conductivity, the hybrid material exhibits a high areal capacitance for a supercapacitor (3200 mF cm-2 at 1 mA cm-2).

8.
Dalton Trans ; 47(16): 5639-5645, 2018 Apr 24.
Artigo em Inglês | MEDLINE | ID: mdl-29619467

RESUMO

Metal-organic frameworks (MOFs) show great advantages as new kinds of active materials for energy storage. In this study, bimetallic metal-organic frameworks (Ni/Co-MOFs) with nanosheet-assembled flower-like structures were synthesized by etching Ni-MOF microspheres in a cobalt nitrate solution. It can be clearly observed that the amount of Co(NO3)2 and etching time play crucial roles in the formation of Ni/Co-MOF nanosheets. The Ni/Co-MOFs were used as electrode materials for supercapacitors and the optimized Ni/Co-MOF-5 exhibited the highest capacitances of 1220.2 F g-1 and 986.7 F g-1 at current densities of 1 A g-1 and 10 A g-1, respectively. Ni/Co-MOF-5 was further sulfurized, and the derived Ni-Co-S electrode showed a higher specific capacitance of 1377.5 F g-1 at a current density of 1 A g-1 and a retention of 89.4% when the current density was increased to 10 A g-1, indicating superior rate capability. Furthermore, Ni/Co-MOF-5 and Ni-Co-S showed excellent cycling stability, i.e. about 87.8% and 93.7% of initial capacitance can be still maintained after 3000 cycles of charge-discharge. More interestingly, the Ni/Co-MOF-5//AC ASC shows an energy density of 30.9 W h kg-1 at a power density of 1132.8 W kg-1, and the Ni-Co-S//AC ASC displays a high energy density of 36.9 W h kg-1 at a power density of 1066.42 W kg-1. These results demonstrate that the as-synthesized bimetallic Ni/Co-MOF nanosheets and their derived nickel-cobalt sulfides have promising applications in electrochemical supercapacitors.

9.
Dalton Trans ; 46(48): 16821-16827, 2017 Dec 12.
Artigo em Inglês | MEDLINE | ID: mdl-29034389

RESUMO

Electrode materials for supercapacitors with one-dimensional porous nanostructures, such as nanowires and nanotubes, are very attractive for high-efficiency storage of electrochemical energy. Herein, ultralong Cu-based porous coordination polymer nanowires (copper-l-aspartic acid) were used as the electrode material for supercapacitors, for the first time. The as-prepared material exhibits a high specific capacitance of 367 F g-1 at 0.6 A g-1 and excellent cycling stability (94% retention over 1000 cycles). Moreover, porous CuO nanotubes were successfully fabricated by the thermal decomposition of this nanowire precursor. The CuO nanotube exhibits good electrochemical performance with high rate capacity (77% retention at 12.5 A g-1) and long-term stability (96% retention over 1000 cycles). The strategy developed here for the synthesis of porous nanowires and nanotubes can be extended to the construction of other electrode materials for more efficient energy storage.

10.
Chin Med J (Engl) ; 130(18): 2163-2169, 2017 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-28836571

RESUMO

Background:: Acute kidney injury (AKI) is the most common and life-threatening systemic complication of rhabdomyolysis. Inflammation plays an important role in the development of rhabdomyolysis-induced AKI. This study aimed to investigate the kidney model of AKI caused by rhabdomyolysis to verify the role of macrophage Toll-like receptor 4/nuclear factor-kappa B (TLR4/NF-κB) signaling pathway. Methods:: C57BL/6 mice were injected with a 50% glycerin solution at bilateral back limbs to induce rhabdomyolysis, and CLI-095 or pyrrolidine dithiocarbamate (PDTC) was intraperitoneally injected at 0.5 h before molding. Serum creatinine levels, creatine kinase, the expression of tumor necrosis factor (TNF)-α, interleukin (IL)-1ß and IL-6, and hematoxylin and eosin stainings of kidney tissues were tested. The infiltration of macrophage, mRNA levels, and protein expression of TLR4 and NF-κB were investigated by immunofluorescence double-staining techniques, reverse transcriptase-quantitative polymerase chain reaction, and Western blotting, respectively. In vitro, macrophage RAW264.7 was stimulated by ferrous myoglobin; the cytokines, TLR4 and NF-κB expressions were also detected. Results:: In an in vivo study, using CLI-095 or PDTC to block TLR4/NF-κB, functional and histologic results showed that the inhibition of TLR4 or NF-κB alleviated glycerol-induced renal damages (P < 0.01). CLI-095 or PDTC administration suppressed proinflammatory cytokine (TNF-α, IL-6, and IL-1ß) production and macrophage infiltration into the kidney (P < 0.01). Moreover, in an in vitro study, CLI-095 or PDTC suppressed myoglobin-induced expression of TLR4, NF-κB, and proinflammatory cytokine levels in macrophage RAW264.7 cells (P < 0.01). Conclusion:: The pharmacological inhibition of TLR4/NF-κB exhibited protective effects on rhabdomyolysis-induced AKI by the regulation of proinflammatory cytokine production and macrophage infiltration.


Assuntos
Lesão Renal Aguda/tratamento farmacológico , NF-kappa B/metabolismo , Rabdomiólise/complicações , Receptor 4 Toll-Like/metabolismo , Lesão Renal Aguda/metabolismo , Animais , Western Blotting , Ensaio de Imunoadsorção Enzimática , Interleucina-1beta/metabolismo , Interleucina-6/metabolismo , Rim/efeitos dos fármacos , Rim/metabolismo , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , NF-kappa B/antagonistas & inibidores , Prolina/análogos & derivados , Prolina/farmacologia , Prolina/uso terapêutico , Pirrolidinas/farmacologia , Pirrolidinas/uso terapêutico , Células RAW 264.7 , Rabdomiólise/metabolismo , Sulfonamidas/farmacologia , Sulfonamidas/uso terapêutico , Tiocarbamatos/farmacologia , Tiocarbamatos/uso terapêutico , Receptor 4 Toll-Like/antagonistas & inibidores , Fator de Necrose Tumoral alfa/antagonistas & inibidores , Fator de Necrose Tumoral alfa/metabolismo
11.
Dalton Trans ; 46(23): 7388-7391, 2017 Jun 13.
Artigo em Inglês | MEDLINE | ID: mdl-28488714

RESUMO

A hierarchical NiO/NiMn-LDH nanosheet array on Ni foam was prepared via a facile two-step approach and exhibited a high specific capacitance (937 F g-1 at 0.5 A g-1) and good cycling stability (91% retention after 1000 cycles at 5 A g-1). The improved electrochemical performance is benefited from the synergistic properties of hierarchical NiO/LDH nanosheet composites on a conductive substrate.

12.
J Phys Condens Matter ; 29(14): 145301, 2017 Apr 12.
Artigo em Inglês | MEDLINE | ID: mdl-28102824

RESUMO

Massless charge carriers in gate potentials modulate graphene quantum well transport in the same way that a electromagnetic wave propagates in optical fibers. A recent experiment by Kim et al (2016 Nat. Phys. 12 1022) reports valley symmetry preserved transport in a graphene carrier guider. Based on a tight-binding model, the valley-resolved transport coefficients are calculated with the method of scattering matrix theory. For a straight potential well, valley-resolved conductance is quantized with a value of 2n + 1 and multiplied by 2e 2/h with integer n. In the absence of disorder, intervalley scattering, only occurring at both ends of the potential well, is weak. The propagating modes inside the potential well are analyzed with the help of band structure and wave function distribution. The conductance is better preserved for a longer carrier guider. The quantized conductance is barely affected by the boundaries of different types or slightly changing the orientation of the carrier guider. For a curved model, the state with momentum closes to the neutral point is more fragile to boundary scattering and the quantized conductance is ruined as well.

13.
Chin Med J (Engl) ; 129(9): 1100-7, 2016 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-27098797

RESUMO

BACKGROUND: Resolvin D1 (RvD1) is a newly found anti-inflammatory bioactive compound derived from polyunsaturated fatty acids. The current study aimed to explore the protective effect of RvD1 on lipopolysaccharide (LPS)-induced acute kidney injury (AKI) and its possible mechanism. METHODS: Both in vivo and in vitro studies were conducted. Male BALB/c mice were randomly divided into control group (saline), LPS group (LPS 5 mg/kg), RvD1 group (RvD1 5 µg/kg + LPS 5 mg/kg), and blockage group (Boc-MLP 5 µg/kg + RvD1 5 µg/kg + LPS 5 mg/kg). Boc-MLP is a RvD1 receptor blocker. The mice were intraperitoneally injected with these drugs and recorded for general condition for 48 h, while the blood and kidneys were harvested at 2, 6, 12, 24, and 48 h time points, respectively (n = 6 in each group at each time point). Human proximal tubule epithelial cells (HK-2) were randomly divided into control group (medium only), LPS group (LPS 5 µg/ml), RvD1 group (RvD1 10 ng/ml + LPS 5 µg/ml), and blockage group (Boc-MLP 10 ng/ml + RvD1 10 ng/ml + LPS 5 µg/ml). The cells were harvested for RNA at 2, 4, 6, 12, and 24 h time points, respectively (n = 6 in each group at each time point). Blood creatinine was tested by using an Abbott i-STAT portable blood gas analyzer. Tumor necrosis factor-α (TNF-α) level was detected by ELISA. Kidney pathology was observed under hematoxylin and eosin (HE) staining and transmission electron microscope (TEM). We hired immune-histological staining, Western blotting, and fluorescence quantitative polymerase chain reaction to detect the expression of RvD1 receptor ALX, nuclear factor-kappa B (NF-κB) signaling pathway as well as caspase-3. Kidney apoptosis was evaluated by TUNEL staining. RESULTS: RvD1 receptor ALX was detected on renal tubular epithelials. Kaplan-Meier analysis indicated that RvD1 improved 48 h animal survival (80%) compared with LPS group (40%) and RvD1 blockage group (60%), while RvD1 also ameliorated kidney pathological injury in HE staining and TEM scan. After LPS stimulation, the mRNA expression of toll-like receptor 4, myeloid differentiation factor 88, and TNF-α in both mice kidneys and HK-2 cells were all up-regulated, while RvD1 substantially inhibited the up-regulation of these genes. Western blotting showed that the phosphorylated-IκB/IκB ratio in LPS group was significantly higher than that in the control group, which was inhibited in the RvD1 group. RvD1 could inhibit the up-regulation of cleaved-caspase-3 protein stimulated by LPS, which was prohibited in RvD1 blockage group. RvD1 group also had a lower proportion of apoptotic nuclei in mice kidney by TUNEL staining compared with LPS group. CONCLUSION: In LPS-induced AKI, RvD1 could decrease TNF-α level, ameliorate kidney pathological injury, protect kidney function, and improve animal survival by down-regulating NF-κB inflammatory signal as well as inhibiting renal cell apoptosis.


Assuntos
Lesão Renal Aguda/prevenção & controle , Apoptose/efeitos dos fármacos , Ácidos Docosa-Hexaenoicos/farmacologia , Lipopolissacarídeos/farmacologia , NF-kappa B/antagonistas & inibidores , Lesão Renal Aguda/induzido quimicamente , Proteínas Adaptadoras de Transdução de Sinal/análise , Animais , Regulação para Baixo , Rim/efeitos dos fármacos , Rim/patologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Fator de Necrose Tumoral alfa/análise
14.
Plasmid ; 79: 15-21, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-25681561

RESUMO

In order to clone PCR products and express them effectively in Escherichia coli, a directional cloning system was constructed by generating a T vector based on pQE-30Xa. The vector was prepared by inserting an XcmI cassette containing an endonuclease XcmI site, a kanamycin selective marker, a multiple-cloning-site (MCS) region and an opposite endonuclease XcmI site into the vector pQE-30Xa. The T vector pQE-T with single overhanging dT residues at both 3' ends was obtained by digesting with the restriction enzyme XcmI. For directional cloning, a BamHI site was introduced to the ends of the PCR products. A BamHI site was also located on the multiple cloning site of pQE-T. The PCR products were ligated with pQE-T. The directionally inserted recombinants were distinguished by using BamHI to digest the recombinants because there are two BamHI sites located on the both sides of PCR fragment. In order to identify the T-vector functions, the 14-3-3-ZsGreen and hRBP genes were amplified and a BamHI site was added to the ends of the genes to confirm this vector by ligation with pQE-T. Results showed that the 14-3-3-ZsGreen and hRBP were cloned to the vector pQE-T directly and corresponding proteins were successfully produced. It was here demonstrated that this directional vector is capable of gene cloning and is used to manipulate gene expression very easily. The methodology proposed here involves easy incorporation of the construct into other vectors in various hosts.


Assuntos
Clonagem Molecular , Desoxirribonucleases de Sítio Específico do Tipo II/genética , Escherichia coli/genética , Genes Bacterianos , Vetores Genéticos , Sequência de Bases , DNA Bacteriano/genética , Eletroforese em Gel de Poliacrilamida , Regulação Bacteriana da Expressão Gênica , Marcadores Genéticos , Canamicina/farmacologia , Dados de Sequência Molecular , Plasmídeos/genética , Reação em Cadeia da Polimerase , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/genética , Análise de Sequência de DNA , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
15.
J Diabetes ; 7(6): 858-63, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-25588466

RESUMO

BACKGROUND: The aim of the present study was to investigate the contribution of maturity-onset diabetes of the young (MODY) genes to the etiology of 14 Chinese MODY families and to assess phenotypic differences between patients with MODY but without a known genetic cause of diabetes (MODYX) and those with early onset type 2 diabetes (T2D). METHODS: The study included 14 MODY probands from unrelated families and 59 patients (age of onset ≤35 years) diagnosed as early onset T2D. A standard meal test and metabolic studies were performed to characterize the clinical features of all patients. All probands with MODY were analyzed for nucleotide variations in promoters, exons, and exon-intron boundaries of 13 known MODY genes by direct DNA sequencing. RESULTS: Mutations in 13 known MODY genes were not present in the 14 Chinese families and they were classified as MODYX. However, different polymorphisms were identified, with I27L (42.9%; 12/28) and S487N (46.4%; 13/28) of hepatocyte nuclear factor 4α (HNF1α/MODY3) being two most frequent polymorphisms. Two new polymorphisms, namely T412I and D504H, were detected in carboxyl ester lipase (CEL/MODY8). Compared with patients with early onset T2D, patients with MODYX were diagnosed with diabetes at a younger age (28.3 ± 6.5 vs 24.3 ± 6.5 years; P < 0.05) and had a lower body mass index (BMI; 28.3 ± 6.1 vs 24.1 ± 4.3 kg/m(2) ; P < 0.01) and homeostatic model assessment of ß-cell function (47.6 [22.2-89.4] vs 18.5 [6.5-33.7]; P < 0.05). CONCLUSION: Herein we report on 14 Chinese families with MODYX and describe its phenotype. Compared with early onset T2D, MODYX is characterized by lower BMI and decreased insulin-secreting capacity.


Assuntos
Diabetes Mellitus Tipo 2/genética , Mutação , Polimorfismo Genético , Adolescente , Adulto , Idade de Início , Grupo com Ancestrais do Continente Asiático/genética , Índice de Massa Corporal , China/epidemiologia , Análise Mutacional de DNA , Diabetes Mellitus Tipo 2/sangue , Diabetes Mellitus Tipo 2/diagnóstico , Diabetes Mellitus Tipo 2/etnologia , Diabetes Mellitus Tipo 2/fisiopatologia , Feminino , Marcadores Genéticos , Predisposição Genética para Doença , Humanos , Insulina/sangue , Células Secretoras de Insulina/metabolismo , Masculino , Fenótipo , Fatores de Risco , Adulto Jovem
16.
Artigo em Chinês | MEDLINE | ID: mdl-27097478

RESUMO

OBJECTIVE: To investigate the prevalence and risk factors of Toxoplasma gondii infection among pregnant women in Wuxi City of Jiangsu Province, so as to provide the evidence for developing the preventive and control interventions of T. gondii infection. METHODS: The anti-Toxoplasma IgM and IgG antibodies were detected by using ELISA in the sera sampled from 3 014 pregnant women from 2011 to 2014, and the pregnant outcomes were followed up. The risk factors of T. gondii infection were identified with questionnaires. RESULTS: Among the 3 014 pregnant women, 215 cases were found positive to anti-Toxoplasma antibody (7.13%), including 49 cases positive to IgM antibody (49/215, 22.79%), and 166 cases positive to IgG antibody (166/215, 77.21%). The follow-up revealed that 46 T. gondii-infected pregnant women developed adverse pregnant outcomes (46/215, 21.40%), including 35 cases positive to IgM antibody (35/46, 76.09%) and 11 cases positive to IgG antibody (11/ 46, 23.9.1%). Of the 275 pregnant women without T. gondii infection, 7 cases were found to have adverse pregnant outcomes (2.55%) , which was significantly lower than that in T. gondii-infected pregnant women (P < 0.01). The univariate analysis showed that the close contact with animals, liking eating raw meat, liking eating hot pot or barbecue, and tasting raw meat stuffing were important risk factors of T. gondii infection in pregnant women, compared with the uninfected group (all P values < 0.01). CONCLUSIONS: T. gondii infection may lead to adverse pregnant outcomes among pregnant women. Reduction of close contact with animals, development of good diet and hygiene habits and monitoring of T. gondii infection during pregnancy are effective approaches to avoid the development of adverse pregnant outcomes.


Assuntos
Complicações Parasitárias na Gravidez/epidemiologia , Toxoplasmose/epidemiologia , Adulto , Animais , Anticorpos Antiprotozoários/sangue , China/epidemiologia , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Gravidez , Complicações Parasitárias na Gravidez/sangue , Complicações Parasitárias na Gravidez/diagnóstico , Complicações Parasitárias na Gravidez/parasitologia , Resultado da Gravidez , Gestantes , Estudos Soroepidemiológicos , Toxoplasma/imunologia , Toxoplasma/isolamento & purificação , Toxoplasmose/sangue , Toxoplasmose/diagnóstico , Toxoplasmose/parasitologia , Adulto Jovem
17.
World J Gastroenterol ; 20(41): 15413-22, 2014 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-25386091

RESUMO

AIM: To conduct an updated meta-analysis of prospective studies addressing the association between garlic consumption and colorectal cancer. METHODS: Eligible cohort studies were identified by searching MEDLINE (PubMed) and screening the references of related articles published up to October 2013. Meta-analyses were conducted for colorectal cancer in relation to consumption of raw and cooked (RC) garlic and garlic supplements, separately. The summary relative risks (RR) with 95%CI were calculated using fixed-effects or random-effects model depending on the heterogeneity among studies. RESULTS: A total of 5 prospective cohort studies were identified. In contrast to the previous meta-analysis, no significant associations were found between consumption of RC garlic (RR: 1.06; 95%CI: 0.95-1.19) or garlic supplements (RR: 1.12; 95%CI: 0.96-1.31) and risk of colorectal cancer. A non-significant protective effect of garlic supplement intake against colorectal cancer was observed in females (RR: 0.84; 95%CI: 0.64-1.11), but the opposite was the case in males (RR: 1.24; 95%CI: 0.96-1.59). CONCLUSION: Consumption of RC garlic or garlic supplements is not significantly associated with reduced colorectal cancer risk.


Assuntos
Neoplasias Colorretais/prevenção & controle , Dieta , Suplementos Nutricionais , Alho , Neoplasias Colorretais/diagnóstico , Neoplasias Colorretais/epidemiologia , Feminino , Humanos , Masculino , Estudos Prospectivos , Fatores de Proteção , Medição de Risco , Fatores de Risco , Fatores Sexuais
18.
World J Biol Chem ; 5(3): 301-7, 2014 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-25225598

RESUMO

Colorectal cancer is the third most common cancer worldwide. Metastasis is a major cause of colorectal cancer-related death. Mechanisms of metastasis remain largely obscure. MicroRNA is one of the most important epigenetic regulators by targeting mRNAs post-transcriptionally. Accumulated evidence has supported its significant role in the metastasis of colorectal cancer, including epithelial-mesenchymal transition and angiogenesis. Dissecting microRNAome potentially identifies specific microRNAs as biomarkers of colorectal cancer metastasis. Better understanding of the complex network of microRNAs in colorectal cancer metastasis provide new insights in the biological process of metastasis and in the potential targets for colorectal cancer therapies and for diagnosis of recurrent and metastatic colorectal cancer.

19.
BMC Cancer ; 11: 494, 2011 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-22111914

RESUMO

BACKGROUND: Laparoscopy-assisted surgery, fast-track perioperative treatment are both increasingly used in colorectal cancer treatment, for their short-time benefits of enhanced recovery and short hospital stays. However, the benefits of the integration of the Laparoscopy-assisted surgery, fast-track perioperative treatment, and even with the Xelox chemotherapy, are still unknown. In this study, the three treatments integration is defined as "Fast Track Multi-Discipline Treatment Model" for colorectal cancer and this model extends the benefits to the whole treatment process of colorectal cancer. The main purpose of the study is to explore the feasibility of "Fast Track Multi-Discipline Treatment" model in treatment of colorectal cancer. METHODS: The trial is a prospective randomized controlled study with 2 × 2 balanced factorial design. Patients eligible for the study will be randomized to 4 groups: (I) Laparoscopic surgery with fast track perioperative treatment and Xelox chemotherapy; (II) Open surgery with fast track perioperative treatment and Xelox chemotherapy; (III) Laparoscopic surgery with conventional perioperative treatment and mFolfox6 chemotherapy; (IV) Open surgery with conventional perioperative treatment and mFolfox6 chemotherapy. The primary endpoint of this study is the hospital stays. The secondary endpoints are the quality of life, chemotherapy related adverse events, surgical complications and hospitalization costs. Totally, 340 patients will be enrolled with 85 patients in each group. CONCLUSIONS: The study initiates a new treatment model "Fast Track Multi-Discipline Treatment" for colorectal cancer, and will provide feasibility evidence on the new model "Fast Track Multi-Discipline Treatment" for patients with colorectal cancer. TRIAL REGISTRATION: ClinicalTrials.gov: NCT01080547.


Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Protocolos Clínicos , Neoplasias Colorretais/terapia , Laparoscopia/métodos , Protocolos de Quimioterapia Combinada Antineoplásica/administração & dosagem , Desoxicitidina/administração & dosagem , Desoxicitidina/análogos & derivados , Fluoruracila/administração & dosagem , Fluoruracila/análogos & derivados , Humanos , Tempo de Internação , Leucovorina/administração & dosagem , Estudos Prospectivos
20.
Zhejiang Da Xue Xue Bao Yi Xue Ban ; 36(6): 620-5, 2007 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-18067239

RESUMO

Th17(T helper 17 cell), a newly discovered subset of T cells is associated with IL-23 and characterized by production of IL-17, the functions of which are distinct from those of Th1, Th2 and Treg subsets. The development of Th17 cells can be promoted by TGF-beta1, IL-6, and IL-23; but inhibited by IFN-gamma, IL-4 and Socs3. It is clear that Th17 cells have protective effects on body by facilitating the pro-inflammatory responses. On the other hand, the role of Th17 cells in the pathophysiology of autoimmune diseases has been described.


Assuntos
Doenças Autoimunes , Interleucina-17/biossíntese , Subpopulações de Linfócitos T/imunologia , Linfócitos T Auxiliares-Indutores/imunologia , Doenças Autoimunes/imunologia , Doenças Autoimunes/fisiopatologia , Interleucina-17/imunologia , Interleucina-23/biossíntese , Interleucina-23/genética , Subpopulações de Linfócitos T/fisiologia , Linfócitos T Auxiliares-Indutores/classificação , Linfócitos T Auxiliares-Indutores/citologia , Fator de Crescimento Transformador beta1/biossíntese , Fator de Crescimento Transformador beta1/genética
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