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1.
Water Sci Technol ; 85(8): 2277-2294, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35486455

RESUMO

Recently, urban waterlogging prevention and treatment of black-odorous rivers have become a social concern and the upgradation of drainage system and the development of river runoff pollution control projects have accelerated. The use of deep tunnels to upgrade old drainage systems and achieve pollution control-related engineering designs has complicated the drainage system operation control. The traditional operation control mainly relies on human experience or model simulation. This study provides a perspective of machine learning for controlling the operation of the drainage system and exploring whether the operation suggestions regarding facilities in this system can be given in real time while relying only on real-time data and avoiding the complex model simulation process. Herein, five drainage systems were used as examples: the initial water level of a pipeline, key point water level flow, pump station front pool water level, and most unfavorable point water level were selected as relevant variables and four machine-learning discrimination methods were used for to analyze the weir-lowering operation of a deep tunnel. This study found that the average error rate of the linear discrimination method was <10%, thereby exhibiting satisfactory performance. This study provides insights for improving the operation of complex drainage systems.


Assuntos
Rios , Movimentos da Água , Humanos , Aprendizado de Máquina , Água , Poluição da Água/prevenção & controle
2.
BMC Cancer ; 22(1): 380, 2022 Apr 09.
Artigo em Inglês | MEDLINE | ID: mdl-35397600

RESUMO

BACKGROUND: Hepatocellular carcinoma (HCC) is the third-most deadly cancer worldwide. More breakthroughs are needed in the clinical practice for liver cancer are needed, and new treatment strategies are required. This study aims to determine the significant differences in genes associated with LIHC and further analyze its prognostic value further. METHODS: Here, we used the TCGA-LIHC database and the profiles of GSE25097 from GEO to explore the differentially co-expressed genes in HCC tissues compared with paratumor (or healthy) tissues. Then, we utilized WGCNA to screen differentially co-expressed genes. Finally, we explored the function of FYN in HCC cells and xenograft tumor models. RESULTS: We identified ten hub genes in the protein-protein interaction (PPI) network, but only three (COLEC10, TGFBR3, and FYN) appeared closely related to the prognosis. The expression of FYN was positively correlated with the prognosis of HCC patients. The xenograft model showed that overexpression of FYN could significantly inhibit malignant tumor behaviors and promote tumor cell apoptosis. CONCLUSION: Thus, FYN may be central to the development of LIHC and maybe a novel biomarker for clinical diagnosis and treatment.


Assuntos
Carcinoma Hepatocelular , Neoplasias Hepáticas , Proteínas Proto-Oncogênicas c-fyn , Animais , Biomarcadores Tumorais/genética , Biomarcadores Tumorais/metabolismo , Carcinoma Hepatocelular/patologia , Colectinas/genética , Colectinas/metabolismo , Biologia Computacional , Perfilação da Expressão Gênica , Regulação Neoplásica da Expressão Gênica , Redes Reguladoras de Genes , Humanos , Neoplasias Hepáticas/patologia , Prognóstico , Proteínas Proto-Oncogênicas c-fyn/genética , Proto-Oncogenes
3.
Front Genet ; 13: 827277, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35356424

RESUMO

The Hexi Corridor was an important arena for culture exchange and human migration between ancient China and Central and Western Asia. During the Han Dynasty (202 BCE-220 CE), subsistence strategy along the corridor shifted from pastoralism to a mixed pastoralist-agriculturalist economy. Yet the drivers of this transition remain poorly understood. In this study, we analyze the Y-chromosome and mtDNA of 31 Han Dynasty individuals from the Heishuiguo site, located in the center of the Hexi Corridor. A high-resolution analysis of 485 Y-SNPs and mitogenomes was performed, with the Heishuiguo population classified into Early Han and Late Han groups. It is revealed that (1) when dissecting genetic lineages, the Yellow River Basin origin haplogroups (i.e., Oα-M117, Oß-F46, Oγ-IMS-JST002611, and O2-P164+, M134-) reached relatively high frequencies for the paternal gene pools, while haplogroups of north East Asian origin (e.g., D4 and D5) dominated on the maternal side; (2) in interpopulation comparison using PCA and Fst heatmap, the Heishuiguo population shifted from Southern-Northern Han cline to Northern-Northwestern Han/Hui cline with time, indicating genetic admixture between Yellow River immigrants and natives. By comparison, in maternal mtDNA views, the Heishuiguo population was closely clustered with certain Mongolic-speaking and Northwestern Han populations and exhibited genetic continuity through the Han Dynasty, which suggests that Heishuiguo females originated from local or neighboring regions. Therefore, a sex-biased admixture pattern is observed in the Heishuiguo population. Additionally, genetic contour maps also reveal the same male-dominated migration from the East to Hexi Corridor during the Han Dynasty. This is also consistent with historical records, especially excavated bamboo slips. Combining historical records, archeological findings, stable isotope analysis, and paleoenvironmental studies, our uniparental genetic investigation on the Heishuiguo population reveals how male-dominated migration accompanied with lifestyle adjustments brought by these eastern groups may be the main factor affecting the subsistence strategy transition along the Han Dynasty Hexi Corridor.

4.
J Oncol ; 2022: 8264059, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35237324

RESUMO

BACKGROUND: Aberrant expression of tripartite motif 11 (TRIM11) and the Wnt/ß-catenin pathway are essential for facilitating tumorigenesis and progression in multiple types of cancer. AIM: To investigate the molecular changes linking the dysregulation of TRIM11 and Wnt/ß-catenin pathway activation in gastric cancer (GC) progression. METHODS: The expression levels of TRIM11 were detected in GC tissues and cells by immunohistochemistry and western blotting. The role of TRIM11 in the growth, proliferation, and invasion of gastric cancer cells was observed by a series of cell functional experiments and further verified in vivo. Co-immunoprecipitation (Co-IP), immunofluorescence, cycloheximide, and western blotting assays and other experiments were conducted to explore the mechanisms of TRIM11 underlying the regulation of the Wnt/ß-catenin pathway. For further verification, rescue experiments were performed by cotransfection of TRIM11 and Axin1 siRNA in GC cells. RESULTS: Using Co-IP assays, we identified TRIM11 as a potent binding partner of Axin1 in GC cells. Elevated TRIM11 levels were significantly correlated with unfavorable clinical outcomes and poor survival in patients with GC. In addition, TRIM11 promoted the cell proliferation and invasion capacities of GC cells in vitro and tumor growth in vivo. Mechanistic investigations revealed that TRIM11 destabilized Axin1 protein by interacting with Axin1, thus inducing the activation of the Wnt/ß-catenin pathway. Moreover, we found that the oncogenic effects of TRIM11 on GC cells were partly mediated by suppression of Axin1. Furthermore, the protein expression of TRIM11 and Axin1 was negatively correlated in GC tissues. CONCLUSION: Collectively, our findings not only establish a pivotal TRIM11-Axin1-ß-catenin axis in driving GC progression but also indicate that TRIM11 serves as a valuable therapeutic target for the treatment of GC patients.

5.
Chemistry ; 28(26): e202104366, 2022 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-35218098

RESUMO

While concerns about improving recharged afterglow intensity in vivo still motivate further exploration, afterglow nanoparticles (AGNP) offer unique optical merit for autofluorescence-free biological imaging. Apart from efforts enhancing the afterglow emission properties of AGNP, improving afterglow excitation response to visible or near infrared light is important but has lacked success. Dye sensitization has been used to improve the optical response of photovoltaic nanomaterials and to enhance upconversion luminescence efficiency. This concept has recently been expanded and applied to AGNPs. As a new multifunctional nanoprobe, such dye-sensitized AGNP takes advantage of both high spatial resolution fluorescence imaging and sensitive afterglow imaging. This Concept introduces the background, the concept, mechanism, and related imaging application, as well as reviewing existing challenges and proposing future developmental directions for the dye-sensitized AGNPs.


Assuntos
Luminescência , Nanopartículas , Raios Infravermelhos , Imagem Óptica/métodos
6.
Sci Total Environ ; 822: 153620, 2022 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-35124067

RESUMO

Phosphate, as an important non-renewable agricultural resource, needs to be recovered from wastewater at mg/L scale. Calcite carbonate fine powder was used in P-recovery but could only work in recovering high concentration phosphate. Herein, a new strategy is explored using in situ-formed CaCO3 microspheres (CaCO3-in situ) to realize efficient and fast P-recovery by adding CaCl2 and Na2CO3 solution into low phosphate water (10 mg-P/L). We find that freshly in situ-formed CaCO3 nanoparticles can capture phosphate ions very efficiently and self-assemble into composite CaCO3 microspheres consisting of vaterite and calcite phases. Phosphate ions are possibly immobilized between CaCO3 nanoparticles which stimulate the formation of metastable vaterite CaCO3. Under optimized conditions (Ca/P molar ratio, 6/1), 98% of phosphate can be recovered with a rather low residual phosphate level of ~0.2 mg-P/L within only 30 min which is much time-saving than existing methods. Importantly, superior class P-fertilizer can be obtained with P2O5 content of 20.8 wt% using this novel CaCO3-in situ recovery strategy, which is 4 times as high as that using prepared calcite CaCO3 nanoparticles. The yield of pakchoi, a fast-growing vegetable, was increased by 58.9% (fresh weight) when using the prepared CaCO3-in situ-P fertilizer. This strategy provides a new way of recycling low concentration phosphate while producing high quality fertilizer.


Assuntos
Carbonato de Cálcio , Fertilizantes , Microesferas , Fosfatos , Água
7.
Curr Microbiol ; 79(2): 49, 2022 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-34982234

RESUMO

Streptococcus pneumoniae can cause several diseases including otitis media, sinusitis, pneumonia, sepsis and meningitis. The introduction of pneumococcal vaccines has changed the molecular epidemiological and antibiotic resistance profiles of related diseases. Analysis of molecular patterns and genome sequences of clinical strains may facilitate the identification of novel drug resistance mechanism. Three multidrug resistance 19A isolates were verified, serotyped and the complete genomes were sequenced combining the Pacific Biosciences and the Illumina Miseq platform. Genomic annotation revealed that similar central networks were found in the clinical isolates, and Mauve alignments indicated high similarity between different strains. The pan-genome analysis showed the shared and unique cluster in the strains. Mobile elements were predicted in the isolates including prophages and CRISPER systems, which may participate in the virulence and antibiotic resistance of the strains. The presence of 31 virulence factor genes was predicted from other pathogens for PRSP 19339 and 19343, while 30 for PRSP 19087. Meanwhile, 33 genes antibiotic resistance genes were predicted including antibiotic resistance genes, antibiotic-target genes and antibiotic biosynthesis genes. Further analysis of the antibiotic resistance genes revealed new mutations in the isolates. By comparative genomic analysis, we contributed to the understanding of resistance mechanism of the clinical isolates with other serotype strains, which could facilitate the concrete drug resistance mechanism study.


Assuntos
Preparações Farmacêuticas , Infecções Pneumocócicas , Antibacterianos/farmacologia , Genômica , Humanos , Testes de Sensibilidade Microbiana , Penicilinas , Sorogrupo , Sorotipagem , Streptococcus pneumoniae/genética
8.
Phytochemistry ; 195: 113059, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-34933209

RESUMO

Bletilla striata is a precious traditional Chinese medical herb with a wide range of applications in pharmacological and cosmetic fields. Because of the shortage of resources, Bletilla ochracea and Bletilla formosanare are also used as the substitutes. To distinguish the differences and homologies, the typical morphologic and microscopic characteristics of them were compared, and a UPLC fingerprint analysis coupled with chemometric methods were developed for characterization and quality evaluation of Bletillae Rhizoma. Gastrodin, protocatechuic acid, gymnoside V, blestrianol A, coelonin, gymnosides Ⅸ and batatasin II were identified as the potential chemical markers for comprehensive quality evaluation by ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). The anti-melanogenic activities of the three species were also compared for the first time in vivo using the zebrafish model, the results suggested that B. striata and its two substitutes had obvious anti-melanogenic activities, and they were not-toxic at depigmenting doses. Molecular docking studies revealed batatasin III, blestrianol A, coelonin, and gastrodin were possible multitarget compounds associated with melanogenesis suppression, which are important for their potential future medical application.


Assuntos
Melaninas , Orchidaceae , Animais , Simulação de Acoplamento Molecular , Rizoma , Peixe-Zebra
11.
Sci Total Environ ; 769: 144676, 2021 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-33485194

RESUMO

Perchlorate and hexavalent chromium (Cr(VI)) are common cocontaminants in aquatic environments due to their high water solubility, stability, mobility, and some coapplications. However, few studies have investigated their combined toxicity to organisms. In this work, we studied the acute and chronic toxicities of perchlorate and Cr(VI), alone and in combination, with survival, growth, and reproduction as endpoints using Daphnia carinata as a model organism. For a single contaminant, Cr(VI) was found to be more toxic than perchlorate to D. carinata not only in terms of survival but also in terms of growth and reproduction. In regard to the combined pattern, the interactive effects on survival, growth, and reproduction were mainly additivity, antagonism, and synergism, respectively, suggesting that the interactive response of perchlorate and Cr(VI) is endpoint-specific. Due to significant synergism, over 21 days of observation, the inhibition of 0.1 mg/L perchlorate and 0.2 mg/L Cr(VI) on cumulative offspring per female in the first seven broods reached 63.9 ± 3.6%, suggesting that long-term exposure to perchlorate and Cr(VI) at environmentally relevant concentrations may affect D. carinata reproduction in the natural environment. Our results will be significant for understanding the complicated combined toxicity of perchlorate and Cr to aquatic organisms.


Assuntos
Daphnia , Percloratos , Animais , Cromo/toxicidade , Feminino , Percloratos/toxicidade , Reprodução
12.
FASEB J ; 35(2): e21226, 2021 02.
Artigo em Inglês | MEDLINE | ID: mdl-33236397

RESUMO

The Wiskott-Aldrich syndrome (WAS) is a severe recessive X-linked immunodeficiency resulting from loss-of-function mutations in the WAS gene. Mouse is the only mammalian model used for investigation of WAS pathogenesis. However, the mouse model does not accurately recapitulate WAS clinical phenotypes, thus, limiting its application in WAS clinical research. Herein, we report the generation of WAS knockout (KO) rabbits via embryo co-injection of Cas9 mRNA and a pair of sgRNAs targeting exons 2 and 7. WAS KO rabbits exhibited many symptoms similar to those of WAS patients, including thrombocytopenia, bleeding tendency, infections, and reduced numbers of T cell in the spleen and peripheral blood. The WAS KO rabbit model provides a new valuable tool for preclinical trials of WAS treatment.


Assuntos
Modelos Animais de Doenças , Coelhos , Proteína da Síndrome de Wiskott-Aldrich/genética , Síndrome de Wiskott-Aldrich/genética , Animais , Sistemas CRISPR-Cas , Técnicas de Inativação de Genes/métodos , Fenótipo , Síndrome de Wiskott-Aldrich/patologia
13.
Adv Mater ; 32(49): e2003881, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33145880

RESUMO

Afterglow nanoparticles (AGNPs) possessing inherently long lifetime with tailorable emission colors and uniform size have long been sought due to their time-gating-free high-contrast multiplexing imaging. Herein, via a straightforward template method, it is reported that such multicolor AGNPs can be accomplished. The resultant AGNPs exhibit a series of tunable afterglow emissions, including blue, yellow, green, and white. These multicolor AGNPs are found to be highly bright, enabling them to perform high-contrast multichannel afterglow imaging in vitro and in vivo without the use of any complicated time-gating algorithms or systems, which existing tools are unable to do.


Assuntos
Luminescência , Nanopartículas , Imagem Óptica/métodos , Razão Sinal-Ruído , Cor
14.
Fitoterapia ; 146: 104727, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32950600

RESUMO

Two new atranones T and U (1 and 2), and three known analogues atranone B (3), atranone Q (4), and stachatranone C (5) were isolated from the toxigenic fungus Stachybotrys chartarum. Their structures and absolute configurations were elucidated by spectroscopic data and calculated ECD analyses. The cytotoxicities of all the atranones (1-5) were evaluated against MG-63 human osteosarcoma cell lines. Compound 4 exhibited significant cytotoxic effect against MG-63 with IC50 value of 8.6 µM, being more active than the positive control, 5-FU (IC50 10.4 µM). Morphological features of apoptosis activities were evaluated in 4-treated MG-63 cells. Compound 4 effectively induced apoptosis of MG-63, which was associated with G0/G1-phase cell cycle arrest. Flow cytometric analysis showed that the treatment by 4 significantly induced MG-63 cell apoptosis in a dose-dependent manner.


Assuntos
Antineoplásicos/farmacologia , Apoptose/efeitos dos fármacos , Neoplasias Ósseas/patologia , Osteossarcoma/patologia , Stachybotrys/química , Antineoplásicos/isolamento & purificação , Produtos Biológicos/isolamento & purificação , Pontos de Checagem do Ciclo Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Humanos , Estrutura Molecular
15.
Food Sci Nutr ; 8(8): 4223-4231, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32884703

RESUMO

High-performance liquid chromatographic (HPLC) and flow-injection mass spectrometric (FIMS) fingerprinting profiles were used to differentiate between wild and artificial cultivated Stephaniae tetrandrae Radix samples. HPLC and FIMS fingerprints of 15 wild S. tetrandrae Radix samples and 12 artificial cultivated S. tetrandrae Radix samples were obtained and analyzed with the aid of principal component analysis (PCA). PCA of the fingerprints showed that the chemical differences between wild and artificial cultivated S. tetrandrae Radix samples could be differentiated by either HPLC or FIMS fingerprints. The HPLC fingerprints provided more chemical information but required longer analytical time compared with FIMS fingerprints. This study indicated that the wild samples contained higher concentrations of almost all of the major compounds than the cultivated samples. Three characteristic compounds which were responsible for the differences between the samples were tentatively identified with the aid of MS data. Furthermore, these three compounds, tetrandrine (TET), fangchinoline (FAN), and cyclanoline (CYC), were quantified. The HPLC and FIMS fingerprints combined with PCA could be used for quality assessment of wild and artificial cultivated S. tetrandrae Radix samples.

16.
Infect Drug Resist ; 13: 2571-2578, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32801794

RESUMO

BACKGROUND: Ceftazidime/avibactam (CZA)-resistant carbapenem-resistant Klebsiella pneumoniae (CRKP) infections occur in adults worldwide but are rarely observed in neonates. We evaluated the activities of CZA against CRKP and described the clinical and molecular epidemiology of CZA-resistant CRKP in a NICU prior to CZA approval in China. METHODS: A laboratory-based surveillance of CRKP was conducted from July 2017 to June 2018. Clinical data were initially reviewed. Antimicrobial susceptibility was determined by the broth microdilution method. CZA-resistant CRKP isolates were submitted to carbapenemase types screening and multilocus sequence typing. RESULTS: Over 23.3% (10/43) of CRKP strains were resistant to CZA, MIC50 and MIC90 values being 0.5 µg/mL and >32µg/mL, respectively. Most neonates shared similar clinical features with cesarean (n=8), preterm birth (n=6), low birth weight (n=5), and exposure to carbapenems/ß-lactam (n=8). All CZA-resistant CRKP isolates were highly resistant to most tested drugs except for polymyxin B (POL) and tigecycline (TGC). CZA-resistant CRKP isolates showed greater sensitivity to amikacin (AMK), nitrofurantoin (NIT), levofloxacin (LVX) and ciprofloxacin (CIP), compared with CZA-sensitive CRKP. All CZA-resistant CRKP isolates harbored carbapenemase genes, blakpc-2 (n=5) being predominant, followed by blaNDM-1 (n=4) and blaNDM-5 (n=2). Among these CZA-resistant CRKP isolates, a total of eight different STs were identified. CRKP harboring KPC belonged to ST1419, ST37 and ST11, while NDM types were assigned to ST784, ST1710, ST37 and ST324. Furthermore, other ß-lactamase genes including blaSHV and blaCTX-M were also found. CONCLUSION: Over 23.3% of CRKP strains isolated from neonates were resistant to CZA. Cesarean, preterm birth, low birth weight, and exposure to carbapenems/ß-lactam were similar clinical features of most neonates with CZA-resistant CRKP. The predominant carbapenemases of CZA-resistant CRKP were KPC-2 and NDM-1, and KPC-2 producing K. pneumoniae assigned into 3 STs, which indicate the genetic diversity of clinical CZA-resistant CRKP isolates.

17.
J Genet Genomics ; 47(5): 249-261, 2020 05 20.
Artigo em Inglês | MEDLINE | ID: mdl-32703661

RESUMO

Interspecies chimera through blastocyst complementation could be an alternative approach to create human organs in animals by using human pluripotent stem cells. A mismatch of the major histocompatibility complex of vascular endothelial cells between the human and host animal will cause graft rejection in the transplanted organs. Therefore, to achieve a transplantable organ in animals without rejection, creation of vascular endothelial cells derived from humans within the organ is necessary. In this study, to explore whether donor xeno-pluripotent stem cells can compensate for blood vasculature in host animals, we generated rat-mouse chimeras by injection of rat embryonic stem cells (rESCs) into mouse blastocysts with deficiency of Flk-1 protein, which is associated with endothelial and hematopoietic cell development. We found that rESCs could differentiate into vascular endothelial and hematopoietic cells in the rat-mouse chimeras. The whole yolk sac (YS) of Flk-1EGFP/EGFP rat-mouse chimera was full of rat blood vasculature. Rat genes related to vascular endothelial cells, arteries, and veins, blood vessels formation process, as well as hematopoietic cells, were highly expressed in the YS. Our results suggested that rat vascular endothelial cells could undergo proliferation, migration, and self-assembly to form blood vasculature and that hematopoietic cells could differentiate into B cells, T cells, and myeloid cells in rat-mouse chimeras, which was able to rescue early embryonic lethality caused by Flk-1 deficiency in mouse.


Assuntos
Blastocisto/citologia , Vasos Sanguíneos/transplante , Quimera/genética , Transplante de Células-Tronco Hematopoéticas , Animais , Blastocisto/metabolismo , Vasos Sanguíneos/metabolismo , Transferência Embrionária , Células-Tronco Embrionárias/transplante , Células-Tronco Hematopoéticas/citologia , Células-Tronco Hematopoéticas/metabolismo , Camundongos , Células-Tronco Pluripotentes/citologia , Células-Tronco Pluripotentes/metabolismo , Ratos
18.
Environ Pollut ; 265(Pt B): 115061, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32599333

RESUMO

Microplastic (MP) pollution has become an area of increasing concern because MPs accumulate various types of pollutants. Many previous studies have explored the interactions between MPs and hydrophobic pollutants. However, little research has been conducted on hydrophilic pollutants, which are of much higher concentration and ubiquitous in environment. Surfactants cause hydrophobic MPs to become hydrophilic, which may significantly enhance their capacities to adsorb hydrophilic pollutants. This study explored the influence of co-existing surfactants on the adsorption of ionic organic pollutants by MPs, and found that the presence of an ionic surfactant could significantly enhance the capacity of polyvinyl chloride (PVC, 0.2 mm) MPs to adsorb pollutants with opposite charges. The Langmuir methylene blue adsorption capacity of PVC could be increased from 172 to 4417 ppm in the presence of a sodium dodecyl benzene sulfonate surfactant. Nonionic surfactants impeded the adsorption of both cationic and anionic pollutants due to the steric resistance of the hydrophilic polyethelene glycol chains. The electrostatic interaction mechanism dominated the interfacial behaviors of ionic pollutants on surfactant-adsorbed MP interfaces. The effects of the surfactants were further verified using four different model pollutants and six surfactants. The adsorption capacities of real environmental MPs, including PVC, polyethylene (PE), polypropylene (PP), and polystyrene (PS), increased by three to twenty-six times. The adsorption properties of MPs may be determined by the presence of co-existing surfactants, rather than their polymer species or additives.


Assuntos
Poluentes Ambientais , Poluentes Químicos da Água , Adsorção , Microplásticos , Plásticos , Tensoativos
19.
Food Res Int ; 134: 109230, 2020 08.
Artigo em Inglês | MEDLINE | ID: mdl-32517923

RESUMO

Mushroom bioactive peptides (MBAPs) refer to bioactive peptides extracted directly or indirectly from mushrooms or their mycelia. Owing to the presence of a large quantity of high-quality proteins, many mushrooms are promising sources of bioactive peptides. The beneficial effects, including antihypertensive, antioxidant, and antimicrobial activities, of MBAPs are being increasingly recognized with regards to health promotion and disease prevention. However, this field is relatively undeveloped and relevant reviews are scarce. Hence, the aim of this review was to present the current research status of MBAPs, focusing on their preparation and biological functions. An insight regarding the direction of future research has been also discussed.


Assuntos
Agaricales/química , Inibidores da Enzima Conversora de Angiotensina/química , Antioxidantes/química , Peptídeos/química , Peptídeos/farmacologia , Inibidores da Enzima Conversora de Angiotensina/farmacologia , Antioxidantes/farmacologia
20.
PLoS Negl Trop Dis ; 14(4): e0008287, 2020 04.
Artigo em Inglês | MEDLINE | ID: mdl-32352979

RESUMO

Clonorchiasis, caused by chronic infection with Clonorchis sinensis (C. sinensis), is an important food-borne parasitic disease that seriously afflicts more than 35 million people globally, resulting in a socioeconomic burden in endemic regions. C. sinensis adults long-term inhabit the microaerobic and limited-glucose environment of the bile ducts. Energy metabolism plays a key role in facilitating the adaptation of adult flukes to crowded habitat and hostile environment. To understand energy source for adult flukes, we compared the component and content of free amino acids between C. sinensis-infected and uninfected bile. The results showed that the concentrations of free amino acids, including aspartic acid, serine, glycine, alanine, histidine, asparagine, threonine, lysine, hydroxylysine, and urea, were significantly higher in C. sinensis-infected bile than those in uninfected bile. Furthermore, exogenous amino acids could be utilized by adult flukes via the gluconeogenesis pathway regardless of the absence or presence of exogenous glucose, and the rate-limiting enzymes, such as C. sinensis glucose-6-phosphatase, fructose-1,6-bisphosphatase, phosphoenolpyruvate carboxykinase, and pyruvate carboxylase, exhibited high expression levels by quantitative real-time PCR analysis. Interestingly, no matter whether exogenous glucose was present, inhibition of gluconeogenesis reduced the glucose and glycogen levels as well as the viability and survival time of adult flukes. These results suggest that gluconeogenesis might play a vital role in energy metabolism of C. sinensis and exogenous amino acids probably serve as an important energy source that benefits the continued survival of adult flukes in the host. Our study will be a cornerstone for illuminating the biological characteristics of C. sinensis and the host-parasite interactions.


Assuntos
Aminoácidos/metabolismo , Bile/parasitologia , Clonorchis sinensis/crescimento & desenvolvimento , Clonorchis sinensis/metabolismo , Animais , Bile/química , Gatos , Clonorquíase/parasitologia , Clonorchis sinensis/enzimologia , Clonorchis sinensis/genética , Modelos Animais de Doenças , Metabolismo Energético , Perfilação da Expressão Gênica , Gluconeogênese , Redes e Vias Metabólicas/genética , Ratos
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