Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 508
Filtrar
1.
J Ethnopharmacol ; 282: 114655, 2022 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-34537284

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Inflammatory responses are associated wieh the pathophysiology of depression. Ginsenoside Rb1 (Rb1) exerts antidepressant effect, but the relationship between its activity and inflammation remains unclear. AIM OF THE STUDY: In this study, the antidepressant-like effect and underlying mechanisms of Rb1 were been investigated. MATERIALS AND METHODS: The neuroinflammatory mouse model of lipopolysaccharide (LPS)-induced acute depression-like behavior was employed to detect the action of Rb1. An integrative strategy combining the identification of prototype (Rb1) and its metabolites in vivo with network pharmacology analysis was used to explore therapeutic mechanisms of these ingredients. The putative targets and signalings were experimentally validated. The antidepressant-like effect of F2, the metabolite of Rb1, was firstly evaluated. RESULTS: Rb1 significantly ameliorated LPS-induced depressive-like behavior. Rb1 and its metabolites (Rd, F2, compound K, Rh2, Rg3, PPD) were identified and then a disease-component-target network was established. Experimental validation showed that Rb1 inhibited peripheral and hippocampal inflammation via MAPK/NF-κB signaling. In inflammatory-mediated depression state, Rb1 improved impaired glucocorticoid receptor, suppressed indoleamine 2,3-dioxygenase activity, increased 5-HT level and 5-HT1A receptor expression. Additionally, F2 was firstly discovered to exert antidepressant-like effect, and it existed higher activity than Rb1 against depression. CONCLUSION: The study highlighted the potential of Rb1 and F2 as healthy supplement or agent for inflammation-induced depression.

2.
J Environ Manage ; 302(Pt A): 114056, 2021 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-34741949

RESUMO

Inorganic ligands, ubiquitous in the natural environment, can interact with iron oxide minerals. To date, our knowledge regarding the effects of inorganic ligands on the adsorption properties of antibiotics onto iron oxides is still limited. In this work, the influences of different inorganic ligands (chosen iodate, silicate, and phosphate as the model ligands) on the adsorption of tetracycline (TC) onto hematite were examined. Adsorption isotherms indicated that inorganic ligands inhibited TC adsorption. The observations were attributed to the increase of electrostatic repulsion between anionic species (i.e., TC-) and negatively charged hematite particles as well as the competition between TC- species and inorganic ligand anions for the adsorption sites on hematite surfaces. Interestingly, the inhibitory effects of the three inorganic ligands were in the order of phosphate > silicate > iodate; the trend was stemmed from their differences in the binding affinities to hematite and the molecular size. When the background solutions contained divalent cations (e.g., Ca2+), surface precipitation of Ca-inorganic ligand compounds on hematite was another important mechanism for the inhibitory effects. Furthermore, adsorption of TC onto hematite with or without inorganic ligands was strongly affected by solution pH, which was due to the combination of the amphoteric behavior of TC and highly pH-dependent surface charges of the hematite mineral. Current results highlight the critical roles of ubiquitous inorganic ligands in revealing the fate of tetracycline antibiotics in natural systems.

3.
Nat Commun ; 12(1): 6500, 2021 11 11.
Artigo em Inglês | MEDLINE | ID: mdl-34764257

RESUMO

Lung adenocarcinomas (LUAD) arise from precancerous lesions such as atypical adenomatous hyperplasia, which progress into adenocarcinoma in situ and minimally invasive adenocarcinoma, then finally into invasive adenocarcinoma. The cellular heterogeneity and molecular events underlying this stepwise progression remain unclear. In this study, we perform single-cell RNA sequencing of 268,471 cells collected from 25 patients in four histologic stages of LUAD and compare them to normal cell types. We detect a group of cells closely resembling alveolar type 2 cells (AT2) that emerged during atypical adenomatous hyperplasia and whose transcriptional profile began to diverge from that of AT2 cells as LUAD progressed, taking on feature characteristic of stem-like cells. We identify genes related to energy metabolism and ribosome synthesis that are upregulated in early stages of LUAD and may promote progression. MDK and TIMP1 could be potential biomarkers for understanding LUAD pathogenesis. Our work shed light on the underlying transcriptional signatures of distinct histologic stages of LUAD progression and our findings may facilitate early diagnosis.

4.
Chem Sci ; 12(42): 14065-14073, 2021 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-34760190

RESUMO

Heterogeneous Ni-N-C single-atom catalysts (SACs) have attracted great research interest regarding their capability in facilitating the CO2 reduction reaction (CO2RR), with CO accounting for the major product. However, the fundamental nature of their active Ni sites remains controversial, since the typically proposed pyridinic-type Ni configurations are inactive, display low selectivity, and/or possess an unfavorable formation energy. Herein, we present a constant-potential first-principles and microkinetic model to study the CO2RR at a solid-water interface, which shows that the electrode potential is crucial for governing CO2 activation. A formation energy analysis on several NiN x C4-x (x = 1-4) moieties indicates that the predominant Ni moieties of Ni-N-C SACs are expected to have a formula of NiN4. After determining the potential-dependent thermodynamic and kinetic energy of these Ni moieties, we discover that the energetically favorable pyrrolic-type NiN4 moiety displays high activity for facilitating the selective CO2RR over the competing H2 evolution. Moreover, model polarization curves and Tafel analysis results exhibit reasonable agreement with existing experimental data. This work highlights the intrinsic tetrapyrrolic coordination of Ni for facilitating the CO2RR and offers practical guidance for the rational improvement of SACs, and this model can be expanded to explore mechanisms of other electrocatalysis in aqueous solutions.

6.
Mol Cell Biochem ; 2021 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-34687394

RESUMO

Diabetic cardiomyopathy (DCM) is a cardiovascular complication that tends to occur in patients with diabetes, obesity, or insulin resistance, with a higher late mortality rate. Sustained hyperglycemia, increased free fatty acids, or insulin resistance induces metabolic disorders in cardiac tissues and cells, leading to myocardial fibrosis, left ventricular hypertrophy, diastolic and/or systolic dysfunction, and finally develop into congestive heart failure. The close connection between all signaling pathways and the complex pathogenesis of DCM cause difficulties in finding effective targets for the treatment of DCM. It reported that hydrogen sulfide (H2S) could regulate cell energy substrate metabolism, reduce insulin resistance, protect cardiomyocytes, and improve myocardial function by acting on related key proteins such as differentiation cluster 36 (CD36) and glucose transporter 4 (GLUT4). In this article, the relative mechanisms of H2S in alleviating metabolic disorders of DCM were reviewed, and how H2S can better prevent and treat DCM in clinical practice will be discussed.

7.
Virol J ; 18(1): 213, 2021 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-34715890

RESUMO

BACKGROUND: The fifth wave of H7N9 avian influenza virus caused a large number of human infections and a large number of poultry deaths in China. Since September 2017, mainland China has begun to vaccinate poultry with H5 + H7 avian influenza vaccine. We investigated the avian influenza virus infections in different types of live poultry markets and samples before and after genotype H5 + H7 vaccination in Nanchang, and analyzed the changes of the HA subtypes of AIVs. METHODS: From 2016 to 2019, we monitored different live poultry markets and collected specimens, using real-time reverse transcription polymerase chain reaction (RT-PCR) technology to detect the nucleic acid of type A avian influenza virus in the samples. The H5, H7 and H9 subtypes of influenza viruses were further classified for the positive results. The χ2 test was used to compare the differences in the separation rates of different avian influenza subtypes. RESULTS: We analyzed 5,196 samples collected before and after vaccination and found that the infection rate of AIV in wholesale market (21.73%) was lower than that in retail market (24.74%) (P < 0.05). Among all the samples, the positive rate of sewage samples (33.90%) was the highest (P < 0.001). After vaccination, the positive rate of H5 and H7 subtypes decreased, and the positive rate of H9 subtype and untypable HA type increased significantly (P < 0.001). The positive rates of H9 subtype in different types of LPMs and different types of samples increased significantly (P < 0.01), and the positive rates of untypable HA type increased significantly in all environmental samples (P < 0.05). CONCLUSIONS: Since vaccination, the positive rates of H5 and H7 subtypes have decreased, but the positive rates of H9 subtypes have increased to varying degrees in different testing locations and all samples. This results show that the government should establish more complete measures to achieve long-term control of the avian influenza virus.

8.
Rev Sci Instrum ; 92(10): 104707, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34717429

RESUMO

Surface nuclear magnetic resonance (SNMR) technology is widely used for hydrological investigations. At high ambient noise levels, the use of a pre-polarization field can enhance the amplitude of the free induced decay (FID) signal. However, the spatial distribution of the pre-polarization field is inhomogeneous, and so it is vital to optimize the shutdown ramp and time for all combinations of the pre-polarization field amplitude and relative orientation. For this purpose, we designed a rapid and adiabatic shutdown circuit for pre-polarization surface nuclear magnetic resonance (PSNMR) systems. In the initial stage of the shutdown process, the magnetic field energy stored in the direct current transmitting coil is transferred to the capacitor by a semi-controlled full bridge circuit. At the end of the shutdown period, the pre-polarization current decays exponentially toward zero. The shutdown process can be controlled by adjusting the circuit parameters. The shutdown ramp and time of the designed circuit are verified through a series of simulations and experiments. As the initial voltage of the capacitor increases from 65 to 300 V, the initial amplitude of the exponential decay of the pre-polarization current increases from 15 to 48 A and the shutdown time decreases from 1.4 to 1 ms. Our design scheme provides a more reliable concept for the shutdown process in the case of multiple transmitting coils. This allows a larger gain of the FID signal to be obtained in specific PSNMR applications when pre-polarization currents with slightly different channel parameters need to be shutdown uniformly.

9.
Am J Trop Med Hyg ; 2021 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-34715677

RESUMO

This article aims to understand the changes in the detection rates of H5, H7, and H9 subtypes of avian influenza viruses (AIVs) in the live poultry markets (LPMs) in Nanchang City, Jiangxi Province, before and after the outbreak of the COVID-19. From 2019 to 2020, we monitored the LPM and collected specimens, using real-time reverse transcription polymerase chain reaction technology to detect the nucleic acid of type A AIV in the samples. The H5, H7, and H9 subtypes of influenza viruses were further classified for positive results. We analyzed 1,959 samples before and after the outbreak and found that the positive rates of avian influenza A virus (39.69%) and H9 subtype (30.66%) after the outbreak were significantly higher than before the outbreak (26.84% and 20.90%, respectively; P < 0.001). In various LPMs, the positive rate of H9 subtypes has increased significantly (P ≤ 0.001). Positive rates of the H9 subtype in duck, fecal, daub, and sewage samples, but not chicken samples, have increased to varying degrees. This study shows that additional measures are needed to strengthen the control of AIVs now that LPMs have reopened after the relaxing of COVID-19-related restrictions.

10.
ACS Appl Mater Interfaces ; 13(41): 48709-48719, 2021 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-34636242

RESUMO

Hierarchically porous-structured materials show tremendous potential for catalytic applications. In this work, a facile method through the combination of three-dimensional (3D) printing and chemical dealloying was employed to synthesize a nanoporous-copper-encapsulating microporous-diamond-cellular-structure (NPC@DCS) catalyst. The developed NPC@DCS catalyst was utilized as a heterogeneous photo-Fenton-like catalyst where its catalytic applications in the remediation of organic wastewater were exemplified. The experimental results demonstrated that the NPC@DCS catalyst possessed a remarkable degradation efficiency in the removal of rhodamine B with a reaction rate of 8.24 × 10-2 min-1 and displayed attractive stability, durability, mineralization capability, and versatility. This work not only manifests the applicability of the proposed NPC@DCS catalyst for wastewater purification in practical applications but also is anticipated to inspire the incorporation of the 3D printing technology and chemical synthesis to design high-performance metal catalysts with tunable hierarchical micro- and nanopores for functional applications.

11.
Artigo em Inglês | MEDLINE | ID: mdl-34606457

RESUMO

We propose a novel framework to efficiently capture the unknown reflectance on a non-planar 3D object, by learning to probe the 4D view-lighting domain with a high-performance illumination multiplexing setup. The core of our framework is a deep neural network, specifically tailored to exploit the multi-view coherence for efficiency. It takes as input the photometric measurements of a surface point under learned lighting patterns at different views, automatically aggregates the information and reconstructs the anisotropic reflectance. We also evaluate the impact of different sampling parameters over our network. The effectiveness of our framework is demonstrated on high-quality reconstructions of a variety of physical objects, with an acquisition efficiency outperforming state-of-the-art techniques.

12.
BMC Pregnancy Childbirth ; 21(1): 725, 2021 Oct 27.
Artigo em Inglês | MEDLINE | ID: mdl-34706683

RESUMO

BACKGROUND: Psychological distress may exert a negative influence on reproductive function of couples at reproductive age. Couples seeking assisted reproductive technology (ART) treatment may have a higher prevalence of psychological distress than fertile couples. However, whether psychological distress is associated with the outcome of ART treatment remains unknown. We aimed to investigate the association of pre-treatment psychological distress and clinical pregnancy rate among infertility couples undergoing in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) treatment. METHODS: This nested case-control study was conducted based on women who underwent their first fresh IVF or ICSI cycle in the Jiangsu Birth Cohort Study (JBC) between November 2015 and January 2019. A total of 150 women who did not obtain clinical pregnancy after first IVF or ICSI fresh embryo transfer were identified as cases, and a total of 300 age matched women who obtained clinical pregnancy were identified as controls. Conditional logistic regression analyses were used to investigate the association between psychological distress and the outcome of first IVF or ICSI treatment, adjusting for multiple potential confounders. RESULTS: No statistically significant association was observed between score of maternal symptoms of psychological distress and clinical pregnancy. Adjusted ORs of logistic regression were 1.00 (95% CI 0.97-1.03) for anxiety, 0.98 (95% CI 0.95-1.02) for depression, and 0.98 (95% CI 0.95-1.01) for perceived stress, respectively. When treat depression and anxiety as categorical variables, 62 (13.8%) were classified as clinical depression, 11 (2.4%) were classified as clinical anxiety, among 450 women in the present study. Psychological distress symptoms were also not associated with clinical pregnancy rate. Adjusted ORs of logistic regression were 0.27 (95% CI 0.03-2.33) for anxiety, 0.88 (95% CI 0.46-1.68) for depression, respectively. CONCLUSIONS: Our findings firstly indicated that psychological distress experienced prior to IVF/ICSI treatment was not associated with clinical pregnancy.

13.
Adv Mater ; : e2102877, 2021 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-34699637

RESUMO

Anisotropy is the characteristic of a material to exhibit variations in its mechanical, electrical, thermal, optical properties, etc. along different directions. Anisotropic materials have attracted great research interest because of their wide applications in aerospace, sensing, soft robotics, and tissue engineering. Three-dimensional (3D) printing provides exceptional advantages in achieving controlled compositions and complex architecture, thereby enabling the manufacture of 3D objects with anisotropic functionalities. Here, a comprehensive review of the recent progress on the 3D printing of anisotropic polymeric materials is presented, including material extrusion, vat photopolymerization, powder bed fusion, and sheet lamination techniques. The state-of-the-art strategies implemented in manipulating anisotropic structures are highlighted with the discussion of material categories, functionalities, potential applications, existing challenges, and future perspectives. This article is protected by copyright. All rights reserved.

14.
Artigo em Inglês | MEDLINE | ID: mdl-34655890

RESUMO

Bufei-Huoxue Capsule (BFHX) was applied to treat chronic obstructive pulmonary disease (COPD) in China. It is composed of Astragali Radix, Paeoniae Radix Rubra, and Psoralea Fructus. A sensitive and reliable ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS/MS) method was developed and validated to quantify the eight main bioactive compounds (psoralen, isopsoralen, neobabaisoflavone, corylin, bavachin, astragaloside IV, ononin and formononetin) in rat plasma after oral administration of BFHX. Osthol was used as an internal standard (IS). Plasma samples were pretreated with methanol to precipitate protein. Chromatographic separation was accomplished using Hypersil GOLDTM C18 column (2.1 mm × 100 mm, 1.9 µm) with a gradient elution profile and a mobile phase consisting of (A) 0.1% formic acid in water and (B) acetonitrile and the flow rate was set at 0.2 mL/min. Multiple reaction monitoring (MRM) mode was applied to perform mass spectrometric analyses. All calibration curves were linear (r > 0.9908) in tested ranges. The intra- and inter-day accuracy and precisions of eight compounds at three different concentration levels were within the acceptable limits. The extraction recovery was within the range of 76.4 âˆ¼ 105.2% and the matrix effects were within the range of 88.3 âˆ¼ 115.0% (RSD ≤ 15.6%). The dilution effects were within the range of 90.2 âˆ¼ 114.9%. These 8 compounds were stable under the tested conditions. So the developed method was valid to evaluate the pharmacokinetic study of eight bioactive compounds after oral administration of BFHX.

15.
Clin Chim Acta ; 523: 325-329, 2021 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-34666031

RESUMO

Atherosclerosis (AS) is the pathological basis of numerous lethal diseases, such as myocardial infarction, heart failure, and stroke. As we know, almost twenty million people worldwide die of the arterial diseases annually. Sestrin2 is a stress-inducing protein, which serves as a guardian by activating AMPK, inhibiting mTOR, and maintaining redox balance beneath various stress environments. A large number of studies show that Sestrin2 would shield the body from injury by stress. Moreover, it has been demonstrated that Sestrin2 is closely connected with AS. Here, this article reviewed the involvement of Sestrin2 in the pathogenesis of AS from four aspects: cellular mechanism, oxidative stress, inflammation, and lipid metabolism. Current evidence reveals that Sestrin2 is a novel target for the prevention and treatment of AS.

16.
ACS Chem Neurosci ; 12(22): 4224-4235, 2021 11 17.
Artigo em Inglês | MEDLINE | ID: mdl-34634903

RESUMO

Parkinson's disease (PD) is a progressive neurological disorder, in which dopaminergic midbrain neurons degenerate, leading to dopamine depletion that is associated with neuronal death. In this Review, we initially describe the pathogenesis of PD and established therapies that unfortunately only delay progression of the disease. With a rapidly escalating incidence in PD, there is an urgent need to develop new therapies that not only halt progression but even reverse degeneration. Biomaterials are playing critical roles in these new therapies which include controlled and site-specific delivery of neurotrophins, increased engraftment of implanted neural stem cells, and redirection of endogenous stem cell populations away from their niche to encourage reparative mechanisms. This Review will therefore cover important design features of biomaterials used in regenerative medicine and tissue engineering strategies targeted at PD.


Assuntos
Células-Tronco Neurais , Doença de Parkinson , Materiais Biocompatíveis , Dopamina , Neurônios Dopaminérgicos , Humanos , Doença de Parkinson/terapia
17.
Front Cell Dev Biol ; 9: 717462, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34589485

RESUMO

Breast cancer (BC) is the most common cancer affecting women and the leading cause of cancer-related deaths worldwide. Compelling evidence indicates that microRNAs (miRNAs) are inextricably involved in the development of cancer. Here, we constructed a novel model, based on miRNA-seq and clinical data downloaded from The Cancer Genome Atlas (TCGA). Data from a total of 962 patients were included in this study, and the relationships among their clinicopathological features, survival, and miRNA-seq expression levels were analyzed. Hsa-miR-186 and hsa-miR-361 were identified as internal reference miRNAs and used to normalize miRNA expression data. A five-miRNA signature, constructed using univariate and multivariate Cox regression, was significantly associated with disease-specific survival (DSS) of patients with BC. Kaplan-Meier (KM) and receiver operating characteristic (ROC) analyses were conducted to confirm the clinical significance of the five-miRNA signature. Finally, a nomogram was constructed based on the five-miRNA signature to evaluate its clinical value. Cox regression analysis revealed that a five-miRNA signature was significantly associated with DSS of patients with BC. KM analysis demonstrated that the signature could efficiently distinguish high- and low-risk patients. Moreover, ROC analysis showed that the five-miRNA signature exhibited high sensitivity and specificity in predicting the prognosis of patients with BC. Patients in the high-risk subgroup who received adjuvant chemotherapy had a significantly lower incidence of mortality than those who did not. A nomogram constructed based on the five-miRNA signature was effective in predicting 5-year DSS. This study presents a novel five-miRNA signature as a reliable prognostic tool to predict DSS and provide theoretical reference significance for individualized clinical decisions for patients with BC.

18.
Langmuir ; 37(40): 11707-11715, 2021 10 12.
Artigo em Inglês | MEDLINE | ID: mdl-34570511

RESUMO

The unconjugated bilirubin (BR) may penetrate through the cell membrane and cause a severe cytotoxicity. However, the molecular mechanism underlying the penetration of BR into the cell membrane is still largely unknown. In this work, we systematically investigate the interaction of BR and a lipid bilayer under different conditions by using all-atom molecular dynamics simulations. It is found that BR at the Z,Z conformation can easily enter into the interior of the lipid bilayer due to its hydrophobicity. However, when BR transforms from the Z,Z conformation to the E,E conformation (after the blue-light emission), its penetration ability is greatly reduced (especially at its ionized state). This study may offer useful physical insights into the effect of phototherapy on the penetration behavior and the cytotoxicity of the unconjugated BR.


Assuntos
Bilirrubina , Bicamadas Lipídicas , Membrana Celular , Conformação Molecular , Simulação de Dinâmica Molecular
19.
Magn Reson Imaging ; 84: 76-83, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34555457

RESUMO

OBJECTIVE: The complex anatomical structures of cerebellopontine angle (CPA) pose a unique challenge to diffusion weighted imaging (DWI). This study aimed to compare the clinical utility of the prototypic 2D turbo gradient- and spin echo-BLADE-DWI (TGSE-BLADE-DWI) with that of readout-segmented echo-planar DWI (RESOLVE-DWI) and single-shot echo-planar DWI (SS-EPI-DWI) to visualize CPA anatomic structures and identify CPA tumors. METHODS: A total of 8 volunteers and 36 patients with pathological CPA tumors were enrolled to perform the three DWI sequences at 3 T. Scan time of TGSE-BLADE-DWI, RESOLVE-DWI and SS-EPI-DWI was 5 min 51 s, 5 min 15 s and 1 min 22 s, respectively. Subjective analysis, including visualization of anatomical structures, geometric distortion, ghosting artifacts, lesion conspicuity, diagnostic confidence, and overall image quality of the three DWI sequences were scored and assessed. Signal-to-noise ratio (SNR), contrast-to-noise ratio (CNR) and apparent diffusion coefficient (ADC) of CPA tumors were measured and compared. RESULTS: A total of 39 lesions were identified, TGSE-BLADE-DWI detected all of them, RESOLVE-DWI 36 and SS-EPI-DWI 27. Significant differences were found in all the subjective parameters among the three DWI sequences (all p < 0.001). TGSE-BLADE-DWI was significantly better than RESOLVE-DWI in visualization of CPA anatomical structures, geometric distortion, ghosting artifacts, lesion conspicuity, diagnostic confidence, and overall image quality (all p < 0.01), and RESOLVE-DWI showed significantly superior performance than SS-EPI-DWI in all parameters (all p < 0.001). CNRs and ADCs were not significantly different among the three DWI sequences (p = 0.355, p = 0.590, respectively). No significant differences were detected between TGSE-BLADE-DWI SNR and RESOLVE-DWI SNR (p = 0.058), or TGSE-BLADE-DWI SNR and SS-EPI-DWI SNR (p = 0.155). CONCLUSION: Compared with RESOLVE-DWI and SS-EPI-DWI, TGSE-BLADE-DWI minimized geometric distortions and ghosting artifacts and demonstrated an improved ability for depicting CPA tumors with better lesion conspicuity. SUMMARY: Geometric distortions and ghosting artifacts are found at bone-air interfaces using conventional diffusion-weighted imaging (DWI), which is a challenge for imaging cerebellopontine angle (CPA) tumors. Our study validated that geometric distortions and ghosting artifacts were not present on 2D turbo gradient- and spin-echo-BLADE-DWI scans, making this technique useful for visualizing CPA anatomic structures and diagnosing CPA tumors.

20.
Oncol Rep ; 46(5)2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34549306

RESUMO

Colorectal cancer (CRC) is a common malignancy with significant prevalence and mortality rates. Circular RNA FOXO3 (circ­FOXO3; hsa_circ_0006404) has been reported to be involved in cancer regulation; however, its role in CRC is yet to be fully elucidated. Therefore, the aim of the present study was to investigate the effect of circ­FOXO3 on CRC progression and identify its underlying mechanism. In the present study, the expression of circ­FOXO3 was investigated in CRC tissues and cells via reverse transcription­quantitative PCR. A Cell Counting Kit­8 and colony formation assays were used to assess cell proliferation. The cell migratory and invasive abilities were detected using the Transwell migration and invasion assays. The luciferase assay and RNA pull­down assay were conducted to verify the relationship of circ­FOXO3, microRNA (miR)­543 and Large tumor suppressor kinase 1 (LATS1). The results demonstrated that circ­FOXO3 expression was downregulated in CRC tissues and cells, and was associated with poor overall survival of patients with CRC. Moreover, circ­FOXO3 was associated with tumor size, distant metastasis, differentiation, lymph node metastasis and TMN stages of patients with CRC. circ­FOXO3 overexpression suppressed CRC cell proliferation, migration and invasion. Luciferase assay and RNA pull­down assay results indicated that circ­FOXO3 functioned as a sponge for miR­543. In addition, circ­FOXO3 increased the expression of LATS1 via sponging miR­543, thus inhibiting CRC cell aggressive features. Collectively, the present results suggested that circ­FOXO3 inhibited CRC metastasis and progression via elevated LATS1 expression by sponging miR­543. Therefore, circ­FOXO3 may be a promising target for CRC therapy.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...