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1.
Colloids Surf B Biointerfaces ; 223: 113141, 2023 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-36682296

RESUMO

Dopamine is a small molecule inspired by the dopamine motif of mussel foot proteins, and PDA is formed by the self-polymerization of dopamine. Under the UV-irradiation,PDA would be oxidized by reactive oxygen species (ROS) which were produced by photocatalytic reactions on TiO2 surfaces,thus regulating the adhesion behavior of endothelial cells (ECs) TiO2 inhibited platelet (Plt) adhesion after UV exposure. Polydopamine (PDA)-TiO2 micropatterns (P-PDA-TiO2) were prepared by magnetron sputtering and photolithography. This micropatterns successfully achieves selective adhesion of Plt and ECs. The selective adhesion of ECs disappears after vacuum reduction. In contrast to conventional cell patterning strategies, P-PDA-TiO2 can easily achieve pattern separation of ECs and Plts and provide a new concept for building complex blood-contacting devices.

2.
Kaohsiung J Med Sci ; 2023 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-36655868

RESUMO

Friend leukemia integration 1 (FLI1) is an ETS transcription factor family member. Here, we identified cg11017065 as the most hyper-methylated cytosine and guanine (CpG) in colorectal cancer (CRC), which belongs to the FLI1 gene. Moreover, integrated bioinformatics prediction and analysis of our cohort showed that FLI1 expression was downregulated and DNA methylation was elevated in CRC. Bioinformatics prediction also indicated that patients overexpressing FLI1 had higher survival rates than those with low FLI1 expression. CRC cells with ectopic expression of FLI1 had reduced invasion, migration, cloning ability and increased apoptosis. Furthermore, DNA-methyltransferase 3b (DNMT3b) was found to be significantly overexpressed in CRC, and low DNMT3b expression predicted a prolonged survival. DNMT3b bound to the FLI1 promoter. Inhibition of DNMT3b increased FLI1 expression and inhibited the malignant phenotype of CRC cells. Inhibition of FLI1 reversed the phenotypic modulation by DNMT3b depletion in vitro and in vivo. In conclusion, our data indicate that DNMT3b potentiates CRC cell proliferation, migration, and invasion through downregulating FLI1.

3.
Genes (Basel) ; 14(1)2023 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-36672909

RESUMO

Intramuscular fat (IMF) is an essential trait closely related to meat quality. The IMF trait is a complex quantitative trait that is regulated by multiple genes. In order to better understand the process of IMF and explore the key factors affecting IMF deposition, we identified differentially expressed mRNA, miRNA, and lncRNA in the longissimus dorsi muscle (LD) between Songliao Black (SL) pigs and Landrace pigs. We obtained 606 differentially expressed genes (DEGs), 55 differentially expressed miRNAs (DEMs), and 30 differentially expressed lncRNAs (DELs) between the SL pig and Landrace pig. Enrichment results from GO and KEGG indicate that DEGs are involved in fatty acid metabolism and some pathways related to glycogen synthesis. We constructed an lncRNA-miRNA-mRNA interaction network with 18 DELs, 11 DEMs, and 42 DEGs. Finally, the research suggests that ARID5B, CPT1B, ACSL1, LPIN1, HSP90AA1, IRS1, IRS2, PIK3CA, PIK3CB, and PLIN2 may be the key genes affecting IMF deposition. The LncRNAs MSTRG.19948.1, MSTRG.13120.1, MSTRG.20210.1, and MSTRG.10023.1, and the miRNAs ssc-miRNA-429 and ssc-miRNA-7-1, may play a regulatory role in IMF deposition through their respective target genes. Our research provides a reference for further understanding the regulatory mechanism of IMF.


Assuntos
MicroRNAs , RNA Longo não Codificante , Animais , Suínos/genética , Perfilação da Expressão Gênica/métodos , RNA Longo não Codificante/genética , RNA Mensageiro/genética , Metabolismo dos Lipídeos/genética , MicroRNAs/genética
4.
Foods ; 12(2)2023 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-36673405

RESUMO

Cadmium contamination and toxicity on plants and human health is a major problem in China. Safe rice production in Cd-contaminated alkaline soils, with acceptably low Cd levels and high yields, remains an important research challenge. To achieve this, a small-scale field experiment with seven different soil amendment materials was conducted to test their effects performance. Two best-performing materials were selected for the large-scale field experiment. Combinations of humic acid, foliar, and/or soil silicon fertilization and deep or shallow plowing were designed. It was found that the combination, including humic acid, soil and foliar silicate fertilization, and shallow plowing (5-10 cm), produced the most desirable results (the lowest soil bioavailable Cd, the lowest grain Cd concentrations, and the highest grain yield). Rice farmers are therefore recommended to implement this combination to attain high grain yield with low Cd concentrations in alkaline soils.

5.
Scand J Gastroenterol ; : 1-8, 2023 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-36651020

RESUMO

BACKGROUND: To analyze the diversity in endoscopic manifestations of Meckel's diverticulum (MD) in adults by using balloon-assisted enteroscopy (BAE) and supply more information on the application of BAE. METHODS: A retrospective study was carried out on adult patients diagnosed with MD by BAE in two tertiary general hospitals in China, from May 2007 to September 2021. The patients were divided into a small bowel bleeding (SBB) group and a control group according to their main symptoms. Clinical charts and endoscopic images were reviewed, analyzed, and summarized. RESULTS: Single diverticulum in the ileum and double-lumen sign were observed in all patients. The SBB group consisted of 51 patients, among which 35 cases of ulcerative lesions, 9 cases of erosive lesions, 9 cases of active bleeding/blood clots, and 4 cases of lumps inside the diverticulum were observed respectively. Majority of ulcerative lesions were inside the diverticulum (23/35). A circumferential stricture inside the diverticulum was discovered in 11 cases, and ulcerative lesions tended to occur at this structure (10/11). In the control group consisting of 15 patients, 1 case of erosive lesions at the orifice edge was observed. The percentage of patients with MD-associated ulcerative lesions was significantly higher in the SBB group than that in the control group (p < 0.001). CONCLUSIONS: The endoscopic manifestations of MD in adults are extraordinarily complex and connected with the patients' primary symptoms. The internal features of MD should be regarded as crucial observational objectives in adult patients.

6.
Adv Healthc Mater ; : e2203306, 2023 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-36708290

RESUMO

Photothermal hydrogel adhesives have yielded promising results for wound closure and infected wound treatment in recent years. However, photothermal hydrogel bioadhesives with on-demand removability without additional nanomaterials-based photothermal agents have rarely been reported in the literature. In this work, an injectable intrinsic photothermal hydrogel bioadhesive with an on-demand removal trait was developed through dynamic crosslinking of gelatin (Gel), tannic (TA) quinone, and borax for closing skin incisions and accelerating methicillin-resistant Staphylococcus aureus (MRSA)-infected wound healing. The TA quinone containing polyphenol and quinone groups with multifunctional adhesiveness and intrinsic photothermal performance conferred the hydrogel adhesive with NIR-responsive antibacterial activity. The cross-linking of pH-sensitive boronic ester (polyphenol-B) and Schiff base bonds endowed the hydrogel with great self-healing capacity and on-demand removability. Moreover, hydrogel possessed good biocompatibility, injectability, and hemostasis. The in vivo experiment in a rat cutaneous incision model and full-thickness MRSA-infected wound model indicated that our smart hydrogel could close wounds efficiently and treat infected ones, demonstrating its superiority in noninvasive treatment of cutaneous incisions and enhancing infected full-thickness wound healing. This article is protected by copyright. All rights reserved.

7.
Comput Med Imaging Graph ; 104: 102184, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36657212

RESUMO

Over the past few years, deep learning-based image registration methods have achieved remarkable performance in medical image analysis. However, many existing methods struggle to ensure accurate registration while preserving the desired diffeomorphic properties and inverse consistency of the final deformation field. To address the problem, this paper presents a novel symmetric pyramid network for medical image inverse consistent diffeomorphic registration. Specifically, we first encode the multi-scale images to the feature pyramids via a shared-weights encoder network and then progressively conduct the feature-level diffeomorphic registration. The feature-level registration is implemented symmetrically to ensure inverse consistency. We independently carry out the forward and backward feature-level registration and average the estimated bidirectional velocity fields for more robust estimation. Finally, we employ symmetric multi-scale similarity loss to train the network. Experimental results on three public datasets, including Mindboggle101, CANDI, and OAI, show that our method significantly outperforms others, demonstrating that the proposed network can achieve accurate alignment and generate the deformation fields with expected properties. Our code will be available at https://github.com/zhangliutong/SPnet.

8.
Inorg Chem ; 62(2): 1054-1061, 2023 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-36606542

RESUMO

The zero-dimensional (0D) (H5O2)(C4H14N2S2)2BiCl8: Sb3+ single crystal is obtained by the cooling crystallization method. Surprisingly, this compound shows reversible photoluminescence (PL) upon H5O2+Cl- removal and insertion. To be specific, the release of H5O2+Cl- resulted in red-orange emission with a very low photoluminescence quantum yield (PLQY). While on the reuptake of it, a bright yellow emission with a nearly 10-fold increase of PLQY was observed. Density functional theory (DFT) calculations and temperature-dependent PL experiments reveal that significant [SbCl6]3- octahedron distortion induced by guest (H5O2+Cl-) removal at the ground state, especially at the excited state, is responsible for the disparate PL performance. Encouragingly, we also found that (C4H14N2S2)2BiCl7: Sb3+ exhibits a fast response (<3 s) to dilute hydrochloric acid with naked-eye perceivable PL color changes, rendering it a potential sensing material for hydrochloric acid.

9.
Front Genet ; 14: 1049454, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36713077

RESUMO

Background: Pancreatic cancer (PC) is a malignant gastrointestinal tumor with a terrible prognosis. Cuproptosis is a recently discovered form of cell death. This study is intended to explore the relationship between cuproptosis-related lncRNAs (CRLncs) signature with the prognosis and the tumor microenvironment (TME) of PC. Methods: Transcript sequencing data of PC samples with clinical information were obtained from the Cancer Genome Atlas (TCGA). Univariate Cox regression analysis and LASSO regression analysis were employed to construct the prognostic signature based on CRLncs associated with PC survival. A nomogram was created according to this signature, and the signaling pathway enrichment was analyzed. Subsequently, we explored the link between this prognostic signature with the mutational landscape and TME. Eventually, drug sensitivity was predicted based on this signature. Results: Forty-six of 159 CRLncs were most significantly relevant to the prognosis of PC, and a 6-lncRNA prognostic signature was established. The expression level of signature lncRNAs were detected in PC cell lines. The AUC value of the ROC curve for this risk score predicting 5-year survival in PC was .944, which was an independent prognostic factor for PC. The risk score was tightly related to the mutational pattern of PC, especially the driver genes of PC. Single-sample gene set enrichment analysis (ssGSEA) demonstrated a significant correlation between signature with the TME of PC. Ultimately, compounds were measured for therapy in high-risk and low-risk PC patients, respectively. Conclusion: A prognostic signature of CRLncs for PC was established in the current study, which may serve as a promising marker for the outcomes of PC patients and has important forecasting roles for gene mutations, immune cell infiltration, and drug sensitivity in PC.

11.
Adv Healthc Mater ; : e2203141, 2023 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-36648020

RESUMO

Intracerebral hemorrhage (ICH) is the most devastating form of stroke, which accounts for 10-15% of cases and causes high morbidity and mortality. With the continuous exploration of the pathological mechanism of ICH, extensive research focusing on ICH therapy has been conducted. However, the traditional treatment methods, such as surgery for removing the hematoma and pharmacotherapy for improving the clearance of the hematoma and neuroprotection, are greatly limited due to their poor practicality and treatment efficiency. The rapid development of drug delivery systems offers an important prospect for treating ICH as they exhibit great versatility, which can improve the pharmacokinetic behavior of drugs in vivo, increase the drug accumulation in specific cell types or tissues, and enhance the therapeutic effect with diminished toxic effect. In this review, the main molecular pathological mechanisms of ICH are comprehensively described and the limitation of traditional pharmacotherapy are also discussed. Then the development based on drug delivery systems for treating ICH is highlighted. Finally, based on these discussions the challenges of drug delivery systems with a view to providing a new feasible path for the treatment of ICH are summarized.

12.
J Biol Chem ; : 102881, 2023 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-36626986

RESUMO

Mutations in genes involved in mitochondrial proline catabolism lead to the rare genetic disorder hyperprolinemia in humans. We have previously reported that mutations of proline catabolic genes in C. elegans impair mitochondrial homeostasis and shorten lifespan, and that these effects surprisingly occur in a diet type-dependent manner. Therefore, we speculated that a specific dietary component may mitigate the adverse effects of defective proline catabolism. Here, we discovered that high dietary glucose, which is generally detrimental to health, actually improves mitochondrial homeostasis and lifespan in C. elegans with faulty proline catabolism. Mechanistically, defective proline catabolism results in a shift of glucose catabolism towards the pentose phosphate pathway (PPP), which is crucial for cellular redox balance. This shift helps to maintain mitochondrial reactive oxygen species (ROS) homeostasis and to extend lifespan, as suppression of the PPP enzyme GSPD-1 prevents the favorable effects of high glucose. Additionally, we demonstrate that this crosstalk between proline and glucose catabolism is mediated by the transcription factor DAF-16. Altogether, these findings suggest that a glucose-rich diet may be advantageous in certain situations, and might represent a potentially viable treatment strategy for disorders involving impaired proline catabolism.

13.
Artigo em Inglês | MEDLINE | ID: mdl-36629847

RESUMO

In order to explore the hearing loss resulting from exposure to continuous or intermittent loud noise. A three-dimensional liquid-solid coupling finite element model of spiral cochlea was established. The reliability of the model was verified, and the stress and amplitude of the basilar membrane of the pivotal structure in cochlea were analyzed. The results show that under the action of the same high-pressure sound, the preferential fatigue area of the cochlear high-frequency area mainly causes fatigue in the cochlear. The safer area is a sound pressure level below 70 dB, while one above 90 dB accelerates damage to the ear.

14.
Hepatology ; 2023 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-36630996

RESUMO

BACKGROUND AND AIMS: Hepatocellular carcinoma (HCC) is an aggressive disease with a poor clinical outcome. Understanding the mechanisms that drive cancer stemness, which we now know is the root cause of therapy failure and tumor recurrence, is fundamental for designing improved therapeutic strategies. This study aims to identify molecular players specific to CD133+HCC to better design drugs that can precisely interfere with cancer stem cells (CSCs) but not normal stem cell function. APPROACH AND RESULTS: Transcriptome profiling of epithelial-specific 'normal' CD133+cells isolated from fetal and regenerating liver against 'HCC' CD133+cells isolated from proto-oncogene driven and inflammation-associated HCC revealed preferential overexpression of SERPINA12 in HCC but not fetal and regenerating liver CD133+cells. SERPINA12 upregulation in HCC is tightly associated with aggressive clinical and stemness features, including survival, tumor stage, cirrhosis, and stemness signatures. Enrichment of SERPINA12 in HCC is mediated by promoter binding of the well-recognized ß-catenin effector TCF7L2 to drive SERPINA12 transcriptional activity. Functional characterization identified a unique and novel role of endogenous SERPINA12 in promoting self-renewal, therapy resistance, and metastatic abilities. Mechanistically, SERPINA12 functioned through binding to GRP78, resulting in a hyperactivated AKT/GSK3ß/ß-catenin signaling cascade, forming a positive feed-forward loop. Intravenous administration of rAVV8-shSERPINA12 sensitized HCC cells to sorafenib and impeded the CSC subset in an immunocompetent HCC mouse model. CONCLUSIONS: Collectively, our findings revealed that SERPINA12 is preferentially overexpressed in epithelial HCC CD133+cells and is a key contributor to HCC initiation and progression by driving an AKT/ß-catenin feed-forward loop.

15.
J Agric Food Chem ; 2023 Jan 26.
Artigo em Inglês | MEDLINE | ID: mdl-36700649

RESUMO

This study aimed to investigate the effects of nitric oxide (NO) and its induced protein S-nitrosylation on the structures and digestion properties of beef myofibrillar protein (MP). The MP was treated with 0, 50, 250, 500, and 1000 µM concentrations of NO-donor S-nitrosoglutathione (GSNO) for 30 min at 37 °C. The results indicated that GSNO treatment significantly decreased the sulfhydryl contents whereas the carbonyl contents increased. Meanwhile, compared with the control group, the surface hydrophobicity, the intrinsic fluorescence intensity, and the α-helix content of proteins were decreased significantly with the enhancement of GSNO concentrations. In addition, 250 µM GSNO treatment increased the gastric digestibility of MP, while the gastrointestinal digestibility and the release of peptides were both inhibited by 500 and 1000 µM GSNO treatments. These data demonstrate that protein S-nitrosylation can affect the in vitro digestion properties of proteins by altering the physicochemical properties and structure of MP.

16.
Appl Geogr ; 152: 102889, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36713474

RESUMO

Studying the commercial dynamics during the COVID-19 recession could help deepen our understanding of how the pandemic damages the commercial economy and how to against the pandemic. This study aims to explore the vulnerability and adaptation of commercial centers using a weekly consumption data of UnionPay cards in Shanghai. A vulnerability index and multiscale geographically weighted regressions (MGWR) are employed. Our results suggest that retail, leisure, and entertainment sectors are less vulnerable to the pandemic at the early stage, when catering, life service, and wholesale sectors are more influenced. Catering, life service, and wholesale sectors were better adapted to the second wave of the pandemic, while the retail and entertainment sectors were even more vulnerable. Further analysis using MGWR models suggests that the commercial centers with higher consumption volume are better adapted to the shock. The diversity of commercial sectors mainly reduces low-level commercial centers' vulnerability to the pandemic. The commercial centers targeting high-end consumers with wider hinterland were less adapted to the pandemic. These research outcomes reveal the disparities in commercial centers' vulnerability against COVID-19 and highlight adaptation's role during the pandemic.

17.
Inorg Chem ; 2023 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-36716166

RESUMO

ns2-Metal halide perovskites have attracted wide attention due to their fascinating photophysical properties. However, achieving high photoluminescence (PL) properties is still an enormous challenge, and the relationship between the lattice environment and ns2-electron expression is still elusive. Herein, an organic-inorganic Bi3+-based halide (C5H14N2)2BiCl6·Cl·2H2O (C5H14N22+ = doubly protonated 1-methylpiperazine) with a six-coordinated structure has been successfully prepared, which, however, exhibits inferior PL properties due to the chemically inert expression of Bi3+-6s2 lone-pair electrons. After reasonably embedding Sb3+ with 5s2 electrons into the lattice of (C5H14N2)2BiCl6·Cl·2H2O, the host lattice environment induces the Sb-Cl moiety to change from the original five-coordinated to six-coordinated structure, thereby resulting in a broad-band yellow emission with a PL efficiency up to 50.75%. By utilizing the host lattice of (C5H14N2)2BiCl6·Cl·2H2O, the expression of Sb3+-5s2 lone-pair electrons is improved and thus promotes the radiative recombination from the Sb3+-3P1 state, resulting in the enhanced PL efficiency. This work will provide an in-depth insight into the effect of the local structure on the expression of Sb3+-5s2 lone-pair electrons.

18.
Mikrochim Acta ; 190(2): 76, 2023 01 28.
Artigo em Inglês | MEDLINE | ID: mdl-36708389

RESUMO

The development of a novel colorimetric method is reported, using vB_YepM_ZN18 phages along with AuPt nanozyme for the sensitive detection of Y. pseudotuberculosis. The phage used in this work has been extracted from hospital sewer water and is highly specific toward Y. pseudotuberculosis. The synthesized AuPt NPs possess peroxidase-like activity, which is suitable in the development of nanozyme based detection system. Furthermore, phages@MB and AuPt@phages are added into the bacterial samples for co-incubation, forming an intercalated complex. The magnetic separation and absorbance analysis of enzymatic reaction are carried out for the detection of targeted bacteria. The proposed method has a limit of detection of 14 CFU/mL, a wide linear range from 2.50 × 101 ~ 2.50 × 107 CFU/mL and the assay completion time is 40 min. Benefitting from the outperformance of this sensor, we have successfully employed the developed sensing platform for the detection of Y. pseudotuberculosis in food industry and hospital specimens.


Assuntos
Bacteriófagos , Yersinia pseudotuberculosis , Colorimetria/métodos
19.
Ecotoxicol Environ Saf ; 250: 114492, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36603487

RESUMO

Urbanization carries essential influences to ecosystem of soil bacteria in coastal cities. Comprehending the patterns and drivers of bacterial diversity are essential to understanding how soil ecosystems respond to environmental change. This study aimed to explore how soil bacterial community (SBC) response to distinct urbanization of coastal cities on composition, assembly process and potential function in Guangdong province, south China. 72 samples from 24 sample sites within 3 cities were included in the study. Soil chemical properties were analyzed, and the bacterial community were investigated by high-throughout sequencing. Proteobacteria and Acidobacteria were the main phyla. Assembly processes remained in stochastic processes and co-occurrence network of SBC kept stable, while urbanization altered SBC by influencing the dominant phyla. The indicators of communities in coastal city soils were the genera gamma_proteobacterium and beta_proteobacterium. Urbanized extent was the non-negligible factor which affected soil bacterial community, despite the total carbon was still the most vital. The impact of urbanization on bacterial communities might follow a non-linear pattern. Faprotax function prediction showed different urbanized coastal city soils share similar metabolic potential. Our study improved our understanding of the response of soil bacterial communities to urbanization in subtropical coastal cities and offered a useful strategy to monitor the ecology risk toward the soil under urbanization.


Assuntos
Ecossistema , Urbanização , Cidades , Solo/química , Microbiologia do Solo , Bactérias/genética , China
20.
Free Radic Biol Med ; 195: 178-191, 2023 02 01.
Artigo em Inglês | MEDLINE | ID: mdl-36587922

RESUMO

Non-alcoholic fatty liver disease (NAFLD) is a highly prevalent liver disease that is closely related to obesity and metabolic disorders. 5-methoxyflavone (5-MF) is a flavonoid with DNA polymerase-ß inhibitory properties. In this study, we explored the effects of 5-MF on NAFLD and its potential mechanisms using oleic acid/palmitic acid-treated HepG2 cells and high-fat diet-fed C57BL/6J mice. Our results showed that 5-MF not only alleviated fat deposition and hepatic steatosis, but also improved oxidative damage. In addition, 5-MF has the effect of alleviating disorders of glucose metabolism and enhancing energy expenditure in HFD-induced obese mice. Mechanistically, reverse screening methods and molecular docking analysis were used in combination, and revealed that cytochrome P450 1A1 (CYP1A1) is the target for 5-MF. Further experiments showed that 5-MF ameliorated triglycerides deposition by inhibiting the enzyme activity and protein expression of CYP1A1. In conclusion, 5-MF provides a novel strategy for the prevention and treatment of high-fat-induced NAFLD.


Assuntos
Hepatopatia Gordurosa não Alcoólica , Camundongos , Animais , Hepatopatia Gordurosa não Alcoólica/metabolismo , Fígado/metabolismo , Citocromo P-450 CYP1A1/genética , Simulação de Acoplamento Molecular , Camundongos Endogâmicos C57BL , Dieta Hiperlipídica/efeitos adversos , Metabolismo dos Lipídeos
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