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FASEB J ; 35(9): e21667, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34405442


Long noncoding RNAs (lncRNAs) are central regulators of the inflammatory response and play an important role in inflammatory diseases. PINT has been reported to be involved in embryonic development and tumorigenesis. However, the potential functions of PINT in the innate immune system are largely unknown. Here, we revealed the transcriptional regulation of inflammatory genes by PINT, whose expression is primarily dependent on the NF-κB signaling pathway in human and mouse macrophage and intestinal epithelial cell lines. Functionally, PINT selectively regulates the expression of TNF-α in basal and LPS-stimulated cells. Mechanistically, PINT acts as a modular scaffold of p65 and EZH2 to coordinate their localization and specify their binding to the target genes. Further, a high expression level of PINT was detected in intestinal mucosal tissues from patients with ulcerative colitis (UC). Together, these findings demonstrate that PINT acts as an activator of inflammatory responses, highlighting the importance of this lncRNA as a potential therapeutic target in infectious diseases and inflammatory diseases.

Proteína Potenciadora do Homólogo 2 de Zeste/metabolismo , Regulação da Expressão Gênica , RNA Longo não Codificante/genética , Fator de Transcrição RelA/metabolismo , Transcrição Genética , Fator de Necrose Tumoral alfa/genética , Animais , Linhagem Celular , Colite Ulcerativa/genética , Colite Ulcerativa/patologia , Citocinas/genética , Humanos , Doenças Inflamatórias Intestinais/genética , Doenças Inflamatórias Intestinais/patologia , Mucosa Intestinal/metabolismo , Mucosa Intestinal/patologia , Macrófagos/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Regiões Promotoras Genéticas/genética , Ligação Proteica , Transcrição Genética/genética
Angew Chem Int Ed Engl ; 60(11): 6137-6144, 2021 Mar 08.
Artigo em Inglês | MEDLINE | ID: mdl-33258189


The photoelectronic properties of quantum dots (QDs) have a critical impact on the performance of quantum-dot-sensitized solar cells (QDSCs). Currently, I-III-VI group QDs have become the mainstream light-harvesting materials in high-performance QDSCs. However, it is still a great challenge to achieve satisfactory efficiency for light-harvesting, charge extraction, and charge collection simultaneously in QDSCs. We design and prepare Zn0.4 Cu0.7 In1.0 Sx Se2-x (ZCISSe) quinary alloyed QDs by cation/anion co-alloying strategy. The critical photoelectronic properties of target QDs, including band gap, conduction band energy level, and density of defect trap states, can be conveniently tailored. Experimental results demonstrate that the ZCISSe quinary alloyed QDs can achieve an ideal balance among light-harvesting, photogenerated electron extraction, and charge-collection efficiencies in QDSCs compared to its single anion or cation quaternary alloyed QD counterparts. Consequently, the quinary alloyed QDs boost the certified efficiency of QDSCs to 14.4 %, which is a new efficiency record for liquid-junction QD solar cells.

FASEB J ; 34(3): 4120-4133, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-31953889


Hepatic stellate cells (HSCs) are the main effectors for various types of hepatic fibrosis, including Schistosome-induced hepatic fibrosis. Multiple inflammatory cytokines/chemokines, such as transforming growth factor-ß1 (TGF-ß1), activate HSCs, and contribute to the development of hepatic fibrosis. MicroRNAs regulate gene expression at the posttranscriptional level and are involved in regulation of inflammatory cytokine/chemokine synthesis. In this study, we showed that soluble egg antigen (SEA) stimulation and Schistosoma japonicum infection downregulate miR-27b expression and increase KH-type splicing regulatory protein (KSRP) mRNA and protein levels in vitro and in vivo. miR-27b regulates the stabilization of TGF-ß1 mRNA through targeting KSRP by interacting with their AU-rich elements in hepatocytes and non-parenchymal cells, which has an effect on the activation of HSCs. Importantly, our results have shown that either knockdown miR-27b or overexpression of KSRP attenuates S. japonicum-induced hepatic fibrosis in vivo. Therefore, our study highlights the crucial role of miR-27b and KSRP in the negative regulation of immune reactions in hepatocyte and non-parenchymal cells in response to SEA stimulation and S. japonicum infection. It reveals that manipulation of miR-27b or KSRP might be a useful strategy not only for treating Schistosome-induced hepatic fibrosis but also for curing hepatic fibrosis in general.

Cirrose Hepática/imunologia , Cirrose Hepática/metabolismo , MicroRNAs/metabolismo , Óvulo/imunologia , Proteínas de Ligação a RNA/metabolismo , Esquistossomose/imunologia , Esquistossomose/metabolismo , Transativadores/metabolismo , Fator de Crescimento Transformador beta1/metabolismo , Animais , Antígenos de Helmintos/farmacologia , Western Blotting , Células Cultivadas , Feminino , Hepatócitos/metabolismo , Humanos , Hibridização in Situ Fluorescente , Camundongos , Camundongos Endogâmicos BALB C , MicroRNAs/genética , Neutrófilos/metabolismo , Células RAW 264.7 , Estabilidade de RNA/genética , Estabilidade de RNA/fisiologia , Proteínas de Ligação a RNA/genética , Reação em Cadeia da Polimerase em Tempo Real , Schistosoma japonicum/imunologia , Schistosoma japonicum/patogenicidade , Transativadores/genética , Fator de Crescimento Transformador beta1/genética
Nan Fang Yi Ke Da Xue Xue Bao ; 39(2): 253-256, 2019 02 28.
Artigo em Chinês | MEDLINE | ID: mdl-30890517


B cell linker (BLNK) is a key linker protein of B cell receptor (BCR) signaling pathway. BLNK participates in the regulation of PLC-γactivity and the activation of Ras pathway through its typical structure and interaction network with other proteins, and is thus widely involved in the regulation of B cell proliferation, differentiation, apoptosis and signal transduction. Furthermore, it is closely related to anaphylactic diseases, multiple sclerosis, chromosomal aneuploidy, aneuglobulinemia, B lymphocytic leukemia and lymphoma. Herein we review the structure and biological function of BLNK and its role in B cell-related diseases. BLNK can cooperate with a series of effective proteins to activate BCR signaling pathway, thereby regulating the development, maturation and function of B cells. The functional mutation of BLNK can destroy the homeostasis of B cells and affect the development and maturation of B cells, which leads to the occurrence of B cell related diseases. A comprehensive understanding of the biological functions of BLNK not only provides insights into the pathogenesis of B cell-related diseases, but also inspires new ideas and helps to find breakthroughs for the treatment of these diseases with BLNK as the therapeutic target.

Proteínas Adaptadoras de Transdução de Sinal/química , Proteínas Adaptadoras de Transdução de Sinal/fisiologia , Linfócitos B/fisiologia , Receptores de Antígenos de Linfócitos B/química , Receptores de Antígenos de Linfócitos B/fisiologia , Proteínas Adaptadoras de Transdução de Sinal/genética , Apoptose , Linfócitos B/citologia , Diferenciação Celular , Proliferação de Células , Humanos , Mutação , Transdução de Sinais , Relação Estrutura-Atividade
J Phys Chem Lett ; 10(2): 229-237, 2019 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-30600681


Intrinsically weak interaction between oil-soluble quantum dots (QDs) and TiO2 in a direct adsorption process limits QD loading and the performance of QD-sensitized solar cells (QDSCs). Herein, the underlying chemistry and mechanisms governing QD adsorption on TiO2 were studied to improve QD loading and cell performance. Experimental results indicate that solvent polarity plays the crucial role in determining QD loading. Compared with single-component solvents, substantially greater QD loading can be realized at the critical point (CP) of bicomponent solvents, where QDs become metastable and start to precipitate. Through this strategy, average efficiency of 12.24% was obtained for ZCISe QDSCs, which is comparable to those based on the capping ligand induced self-assembly route. This report demonstrates the great potential of bicomponent solvents at the CP for high QD loading and excellent cell performance and presents a platform for assembling functional composites with the use of different nanocrystals and substrates.

J Hazard Mater ; 284: 121-9, 2015 Mar 02.
Artigo em Inglês | MEDLINE | ID: mdl-25463225


Raw and biologically treated textile effluents were submerged filtrated using lab-fabricated hollow fiber nanofiltration membrane with a molecular weight cut-off of about 650 g/mol. Permeate flux, chemical oxygen demand (COD) reduction, color removal, membrane fouling, and cleaning were investigated and compared by varying the trans-membrane pressure (TMP) and volume concentrating factor (VCF). It was found that both raw and biologically treated textile effluents could be efficiently treated through submerged nanofiltration. The increase of TMP resulted in a decline in water permeability, COD reduction, color removal, and flux recovery ratio, while the increase of VCF resulted in both increased COD reduction and color removal. Under the TMP of 0.4 bar and VCF of 5.0, fluxes of 1.96 and 2.59 l/m(2)h, COD reductions of 95.7 and 94.2%, color removals of 99.0, and 97.3% and flux recovery ratios of 91.1 and 92.9% could be obtained in filtration of raw and biologically treated effluents, respectively. After filtration, the COD and color contents of the raw effluent declined sharply from 1780 to 325 mg/l and 1.200 to 0.060 Abs/cm, respectively, while for the biologically treated effluent, they decreased from 780 to 180 mg/l and 0.370 to 0.045 Abs/cm, respectively.

Corantes/química , Monitoramento Ambiental/métodos , Nanotecnologia/métodos , Têxteis , Ultrafiltração/métodos , Biodegradação Ambiental , Análise da Demanda Biológica de Oxigênio , Cor , Resíduos Industriais/análise , Membranas Artificiais , Permeabilidade , Indústria Têxtil , Eliminação de Resíduos Líquidos/métodos , Água/química , Poluentes Químicos da Água/química , Purificação da Água/métodos