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1.
J Cell Physiol ; 235(1): 17-25, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31206681

RESUMO

SPTBN1 is a dynamic intracellular nonpleckstrin homology-domain protein, functioning as a transforming growth factor-ß signal transducing adapter protein which is necessary to form Smad3/Smad4 complex. Recently SPTBN1 is considered to be associated with many kinds of cancers. SPTBN1 expression and function differ between different tumor states or types. This review summarizes the recent advances in the expression patterns of SPTBN1 in cancers, and in understanding the mechanisms by which SPTBN1 affects the occurrence, progression, and metastasis of cancer. Identifying SPTBN1 expression and function in cancers will contribute to the clinical diagnosis and treatment of cancer and the investigation of anticancer drugs.

2.
Nanotechnology ; 31(3): 035401, 2020 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-31557745

RESUMO

High capacity and stable anodes are demanded since the current graphite-based anode does not meet the high-performance requirements of emerging Li-ion battery systems. Herein, we present a novel spring-shaped hierarchical SiO2@Co3O4 nanowire composite, which exhibits good Li-storage performance. The special structure is able to effectively accommodate the change in structure during charge-discharge, and the coaxial hierarchical morphology enables rapid Li+ ion and electron transfer. The spring-shaped SiO2@Co3O4 anode exhibits a capacity of 770 mAh g-1, along with a high Coulombic efficiency of 99.8% after 400 cycles. A stable rate performance even after three rounds of measurements is also achievable. In addition, the real-time lithiation of the SiO2@Co3O4 composite is investigated through an in situ transmission electron microscopy technology, which demonstrates the stable structure of the spring-shaped SiO2@Co3O4 composite during the rapid lithiation process.

3.
Int J Mol Sci ; 20(21)2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31683873

RESUMO

R2R3-MYB transcription factors play important roles in the regulation of plant flavonoid metabolites. In the current study, NtMYB3, a novel R2R3-MYB transcriptional factor isolated from Chinese narcissus (Narcissus tazetta L. var. chinensis), was functionally characterized. Phylogenetic analysis indicated that NtMYB3 belongs to the AtMYB4-like clade, which includes repressor MYBs involved in the regulation of flavonoid biosynthesis. Transient assays showed that NtMYB3 significantly reduced red pigmentation induced by the potato anthocyanin activator StMYB-AN1 in agro-infiltrated leaves of tobacco. Over-expression of NtMYB3 decreased the red color of transgenic tobacco flowers, with qRT-PCR analysis showing that NtMYB3 repressed the expression levels of genes involved in anthocyanin and flavonol biosynthesis. However, the proanthocyanin content in flowers of transgenic tobacco increased as compared to wild type. NtMYB3 showed expression in all examined narcissus tissues; the expression level in basal plates of the bulb was highest. A 968 bp promoter fragment of narcissus FLS (NtFLS) was cloned, and transient expression and dual luciferase assays showed NtMYB3 repressed the promoter activity. These results reveal that NtMYB3 is involved in the regulation of flavonoid biosynthesis in narcissus by repressing the biosynthesis of flavonols, and this leads to proanthocyanin accumulation in the basal plate of narcissus.

4.
Sci Rep ; 9(1): 15939, 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31685851

RESUMO

Implementing CNOT operation nonlocally is one of central tasks in distributed quantum computation. Most of previously protocols for implementation quantum CNOT operation only consider implement CNOT operation in one degree of freedom(DOF). In this paper, we present a scheme for nonlocal implementation of hyper-parallel CNOT operation in polarization and spatial-mode DOFs via hyperentanglement. The CNOT operations in polarization DOF and spatial-mode DOF can be remote implemented simultaneously with hyperentanglement assisited by cross-Kerr nonlinearity. Hyper-parallel nonlocal CNOT gate can enhance the quantum channel capacity for distributed quantum computation and long-distance quantum communication. We discuss the experiment feasibility for hyper-parallel nonlocal gate. It shows that the protocol for hyper-parallel nonlocal CNOT operation can be realized with current technology.

5.
J Biol Chem ; 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31690627

RESUMO

Adipose tissue is essential for whole-body glucose homeostasis, with a primary role in lipid storage. It has been previously observed that lactate production is also an important metabolic feature of adipocytes, but its relationship to adipose and whole-body glucose disposal remains unclear. Therefore, using a combination of metabolic labeling techniques, here we closely examined lactate production of cultured and primary mammalian adipocytes. Insulin treatment increased glucose uptake and conversion to lactate, with the latter responding more to insulin than did other metabolic fates of glucose. However, lactate production did not just serve as a mechanism to dispose of excess glucose, since we also observed that lactate production in adipocytes did not solely depend on glucose availability and even occurred independently of glucose metabolism. This suggests that lactate production is prioritized in adipocytes. Furthermore, knocking down lactate dehydrogenase specifically in the fat body of Drosophila flies lowered circulating lactate and improved whole-body glucose disposal. These results emphasize lactate production is an additional metabolic role of adipose tissue beyond lipid storage and release.

6.
Ecotoxicol Environ Saf ; : 109903, 2019 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-31706567

RESUMO

Liming has been widely used to remediate Cd-contaminated acidic soils, but the effects of consecutive liming are still unclear. Four-year liming experiments were conducted to assess the remediation of Cd-contaminated acidic paddy fields in a double rice cropping system. With four-year consecutive liming (quicklime, 2.25 t ha-1 per season), the soil pH was increased by an average of 0.57 units (0.10-1.16 units), while the soil DTPA-Cd and available Fe and Mn were reduced by 9%, 19% and 31% (p < 0.05), respectively. The exchangeable plus water-soluble Cd fraction in soil was reduced by 17%, while the soil carbonate-, Fe/Mn oxide- and organic-bound Cd fractions were increased by 23%, 41% and 10% (p < 0.05), respectively. The Cd in rice grain was reduced by 55% for early rice and 63% for late rice (p < 0.05) and in some cases was reduced to below the Chinese allowable limit (0.2 mg kg-1). High annual fluctuations in rice grain Cd could be caused by variations in the field water regime and in rainfall. With consecutive liming, the soil pH, DTPA-Cd and rice grain Cd varied greatly in the first three seasons and then remained relatively less variable. Meanwhile, soil available nutrients (N, P and K) and rice grain yield were little affected by liming. Soil DTPA-Cd linearly decreased with increasing soil pH, while the reduction of Cd in rice grain logarithmically decreased with increasing soil pH and the reduction in soil DTPA-Cd in the heading stage, indicating potential implications for forecasting rice grain Cd content. Therefore, consecutive liming with quicklime can be recommended for the remediation of Cd-contaminated acidic paddy fields, though supplementary measures are still necessary.

7.
Respir Res ; 20(1): 262, 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31752892

RESUMO

BACKGROUND: Primary pulmonary lymphoepithelioma-like carcinoma (LELC) is a rare tumor and often misdiagnosed as squamous carcinoma. In the current study, clinical characteristics and outcome of primary pulmonary LELC were systematically compared with pulmonary squamous carcinoma. METHODS: Forty-two cases of primary pulmonary LELC and 134 squamous carcinomas were enrolled retrospectively. Characteristic and prognosis difference between the two groups was compared, and the independent prognostic factor for pulmonary LELC was identified as well. RESULTS: In comparison to squamous carcinoma, pulmonary LELC was more common in women with a younger median age and less smokers. LELC seemed to be smaller in diameter on computed tomography (CT) scans than squamous carcinoma, with scarce spiculation and vascular convergence signs. Epstein-Bar virus-encoded RNA (EBER) by in-situ hybridization was detected in 33 LELC cases, among whom 27 ones were positive in serum EBV-DNA examination. LELC patients presented a much longer median progression-free survival (PFS) than squamous carcinoma. Positive serum EBV-DNA, distant lymph node invasion, advanced clinical stage and receiving radiotherapy were correlated with the shorter PFS in LELC patients. However, only positive serum EBV-DNA was the independent PFS predictor. CONCLUSION: Pulmonary LELC looks like distinct from squamous carcinoma. Middle-aged women and nonsmokers are comparatively predominated. CT features of pulmonary LELC are relatively less-malignant. Correspondently, the progression of pulmonary LELC is seemingly favorable than squamous carcinoma and the positive serum EBV-DNA appears to be the predictor of PFS.

8.
Zhongguo Zhen Jiu ; 39(11): 1165-8, 2019 Nov 12.
Artigo em Chinês | MEDLINE | ID: mdl-31724350

RESUMO

OBJECTIVE: To observe the clinical effect of acupuncture at "experienced ten acupoints" for postprandial distress syndrome. METHODS: A total of 62 patients with postprandial distress syndrome were randomly divided into an observation group (31 cases, 5 cases dropping off) and a control group (31 cases, 6 cases dropping off ). Acupuncture was applied at Baihui (GV 20), Zhongwan (CV 12), Qihai (CV 6), Tianshu (ST 25), Neiguan (PC 6), Zusanli (ST 36), Gongsun (SP 4), Danzhong (CV 17) in the observation group. In the control group, 6 non-acupoint points were intervened with shallow puncture. The treatment was given 20 min each time, once every other day, 3 times a week for a total of 4 weeks in the two groups. Symptom index of dyspepsia (SID) and Nepean dyspepsia index (NDI) scores were compared before and after treatment, and the efficacy was evaluated in the two groups. RESULTS: The effective rate in the observation group was 76.9% (20/26), which was higher than 28.0% in the control group (7/25, P<0.01). After treatment, the SID and NDI scores in the two groups were lower than those before treatment (P<0.01, P<0.05), and the SID and NDI scores in the observation group were lower than those in the control group (P<0.01, P<0.05). CONCLUSION: Acupuncture at "experienced ten acupoints" can significantly reduce the symptoms of dyspepsia and improve the quality of life in patients with postprandial distress syndrome.


Assuntos
Terapia por Acupuntura , Dispepsia , Pontos de Acupuntura , Terapia por Acupuntura/métodos , Dispepsia/terapia , Humanos , Qualidade de Vida , Síndrome , Resultado do Tratamento
9.
Biosci Rep ; 39(11)2019 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-31693739

RESUMO

PURPOSE: Nomogram is a widely used tool that precisely predicts individualized cancer prognoses. We aimed to develop and validate a reliable nomogram including serum tumor biomarkers to predict individual overall survival (OS) for patients with resected rectal cancer (RC) and compare the predictive value with the American Joint Committee on Cancer (AJCC) stages. PATIENTS AND METHODS: We analyzed 520 patients who were diagnosed with non-metastatic rectal cancer as training cohort. External validation was performed in a cohort of 11851 patients from the Surveillance, Epidemiology, and End Results (SEER) database. Independent prognostic factors were identified and integrated to build a nomogram using the Cox proportional hazard regression model. The nomogram was evaluated by Harrell's concordance index (C-index) and calibration plots in both training and validation cohort. RESULTS: The calibration curves for probability of 1-, 3-, and 5-year OS in both cohorts showed favorable accordance between the nomogram prediction and the actual observation. The C-indices of the nomograms to predict OS were 0.71 in training cohort and 0.69 in the SEER cohort, which were higher than that of the seventh edition American Joint Committee on Cancer TNM staging system for predicting OS (training cohort, 0.71 vs. 0.58, respectively; P-value < 0.001; validation cohort, 0.69 vs. 0.57, respectively; P-value < 0.001). CONCLUSION: We developed and validated a novel nomogram based on CEA and other factors for predicting OS in patients with resected RC, which could assist clinical decision making and improvement of prognosis prediction for individual RC patients after surgery.

10.
J Neuroeng Rehabil ; 16(1): 143, 2019 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-31744520

RESUMO

BACKGROUND: Neuromuscular electrical stimulation (NMES) is extensively used in stroke motor rehabilitation. How it promotes motor recovery remains only partially understood. NMES could change muscular properties, produce altered sensory inputs, and modulate fluctuations of cortical activities; but the potential contribution from cortico-muscular couplings during NMES synchronized with dynamic movement has rarely been discussed. METHOD: We investigated cortico-muscular interactions during passive, active, and NMES rhythmic pedaling in healthy subjects and chronic stroke survivors. EEG (128 channels), EMG (4 unilateral lower limb muscles) and movement parameters were measured during 3 sessions of constant-speed pedaling. Sensory-level NMES (20 mA) was applied to the muscles, and cyclic stimulation patterns were synchronized with the EMG during pedaling cycles. Adaptive mixture independent component analysis was utilized to determine the movement-related electro-cortical sources and the source dipole clusters. A directed cortico-muscular coupling analysis was conducted between representative source clusters and the EMGs using generalized partial directed coherence (GPDC). The bidirectional GPDC was compared across muscles and pedaling sessions for post-stroke and healthy subjects. RESULTS: Directed cortico-muscular coupling of NMES cycling was more similar to that of active pedaling than to that of passive pedaling for the tested muscles. For healthy subjects, sensory-level NMES could modulate GPDC of both ascending and descending pathways. Whereas for stroke survivors, NMES could modulate GPDC of only the ascending pathways. CONCLUSIONS: By clarifying how NMES influences neuromuscular control during pedaling in healthy and post-stroke subjects, our results indicate the potential limitation of sensory-level NMES in promoting sensorimotor recovery in chronic stroke survivors.

11.
Opt Express ; 27(19): 27202-27214, 2019 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-31674586

RESUMO

During the thinning process of plate-shaped optical parts (PSOP), the release of internal stress would cause the deformation of ultra-thin PSOP, which deteriorates the processed surface figure. The stress-release-induced deformation is hard to be predicted and controlled due to the difficulty in measuring the tiny internal stress in ultra-thin PSOP. In this paper, an analytical model is established to depict the variation of internal stress and deformation during the thinning process. It can be used to calculate the initial internal stress distribution along the thickness according to the deformation and the residual thickness of the sample. Meanwhile, the model can predict the residual stress distribution and deformation in the whole thinning process. The prediction results obtained from the proposed model agree well with the experimental results, and the prediction error is less than 13%. The presented model has great significance for the analysis of the tiny internal stress and then guide the process of making ultra-thin PSOP.

12.
J Neural Eng ; 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31726441

RESUMO

OBJECTIVE: Compound muscle action potential (CMAP) scan has different settings such as stimulus frequency, duration (or pulse width), and the number of stimuli (or steps). This study aims to evaluate the influence of different stimulation protocols on MScanFit, a recently developed approach to motor unit number estimation (MUNE) from CMAP scan. APPROACH: CMAP scan of the first dorsal interosseous (FDI) muscle was performed using four protocols with different pulse widths (0.1 ms, 0.2 ms) and steps (500, 1000) in twelve neurologically intact subjects. For each CMAP scan, the MUNE was derived using MScanFit. MAIN RESULTS: Across all subjects, a significantly higher MUNE was obtained using stimulus pulse width 0.1 ms (500 steps: 107. 7 ± 21.3; 1000 steps: 94.9 ± 22.07) than that using pulse width 0.2 ms (500 steps: 81.8 ± 9.9; 1000 steps: 77.8 ± 16.1) (p < 0.001). However, no significant difference in MUNE was observed using 500 and 1000 steps (p > 0.1). No cross effect of pulse width and steps was found between four different protocols (p > 0.1). SIGNIFICANCE: Given these results, a stimulation protocol of 0.1 ms pulse width and 500 steps is recommended for CMAP scan recording of the FDI muscle toward estimating motor unit number using MScanFit.

13.
Artigo em Inglês | MEDLINE | ID: mdl-31721480

RESUMO

BACKGROUND: Ctns-/- mice, a mouse model of infantile nephropathic cystinosis, exhibit hypermetabolism with adipose tissue browning and profound muscle wasting. Ctns-/- mice are 25(OH)D3 and 1,25(OH)2 D3 insufficient. We investigated whether vitamin D repletion could ameliorate adipose tissue browning and muscle wasting in Ctns-/- mice. METHODS: Twelve-month-old Ctns-/- mice and wild-type controls were treated with 25(OH)D3 and 1,25(OH)2 D3 (75 µg/kg/day and 60 ng/kg/day, respectively) or an ethylene glycol vehicle for 6 weeks. Serum chemistry and parameters of energy homeostasis were measured. We quantitated total fat mass and studied expression of molecules regulating adipose tissue browning, energy metabolism, and inflammation. We measured lean mass content, skeletal muscle fibre size, in vivo muscle function (grip strength and rotarod activity), and expression of molecules regulating muscle metabolism. We also analysed the transcriptome of skeletal muscle in Ctns-/- mice using RNAseq. RESULTS: Supplementation of 25(OH)D3 and 1,25(OH)2 D3 normalized serum concentration of 25(OH)D3 and 1,25(OH)2 D3 in Ctns-/- mice, respectively. Repletion of vitamin D partially or fully normalized food intake, weight gain, gain of fat, and lean mass, improved energy homeostasis, and attenuated perturbations of uncoupling proteins and adenosine triphosphate content in adipose tissue and muscle in Ctns-/- mice. Vitamin D repletion attenuated elevated expression of beige adipose cell biomarkers (UCP-1, CD137, Tmem26, and Tbx1) as well as aberrant expression of molecules implicated in adipose tissue browning (Cox2, Pgf2α, and NF-κB pathway) in inguinal white adipose tissue in Ctns-/- mice. Vitamin D repletion normalized skeletal muscle fibre size and improved in vivo muscle function in Ctns-/- mice. This was accompanied by correcting the increased muscle catabolic signalling (increased protein contents of IL-1ß, IL-6, and TNF-α as well as an increased gene expression of Murf-2, atrogin-1, and myostatin) and promoting the decreased muscle regeneration and myogenesis process (decreased gene expression of Igf1, Pax7, and MyoD) in skeletal muscles of Ctns-/- mice. Muscle RNAseq analysis revealed aberrant gene expression profiles associated with reduced muscle and neuron regeneration, increased energy metabolism, and fibrosis in Ctns-/- mice. Importantly, repletion of 25(OH)D3 and 1,25(OH)2 D3 normalized the top 20 differentially expressed genes in Ctns-/- mice. CONCLUSIONS: We report the novel findings that correction of 25(OH)D3 and 1,25(OH)2 D3 insufficiency reverses cachexia and may improve quality of life by restoring muscle function in an animal model of infantile nephropathic cystinosis. Mechanistically, vitamin D repletion attenuates adipose tissue browning and muscle wasting in Ctns-/- mice via multiple cellular and molecular mechanisms.

14.
Math Biosci Eng ; 17(1): 654-668, 2019 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-31731370

RESUMO

In this paper, a three-dimensional (3D) shape measurement method based on structured light field imaging is proposed, which contributes to the biomedical imaging. Generally, light field imaging is challenging to accomplish the 3D shape measurement accurately, as the slope estimation method based on radiance consistency is inaccurate. Taking into consideration the special modulation of structured light field, we utilize the phase information to substitute the phase consistency for the radiance consistency in epi-polar image (EPI) at first. Therefore, the 3D coordinates are derived after light field calibration, but the results are coarse due to slope estimation error and need to be corrected. Furthermore, the 3D coordinates refinement is performed based on relationship between the structured light field image and DMD image of the projector, which allows to improve the performance of the 3D shape measurement. The necessary light field camera calibration is described to generalize its application. Subsequently, the effectiveness of the proposed method is demonstrated with a sculpture and compared to the results of a conventional PMP system.

15.
Artigo em Inglês | MEDLINE | ID: mdl-31706150

RESUMO

The study is aimed to develop a rapid and efficient supercritical fluid chromatography coupled with diode-array detection (SFC-DAD) method for simultaneous quantification of six flavonoids in Citri Reticulatae Pericarpium (CRP). The chromatographic parameters including stationary phase, mobile phase composition, back pressure, temperature and flow rate, were systematically optimized with single-factor test. The results indicated that the six compounds were baseline separated on an Agilent Zorbax RX-SIL column using gradient elution within 10 min. The optimized mobile phase was constituted of carbon dioxide (CO2) and methanol. And the parameters were set as follows: backpressure, 95 bar; temperature, 45 °C; flow rate, 0.8 mL/min. The optimized method showed satisfactory retention and resolution for the analytes. The method was validated to demonstrate its selectivity, linearity, precision, accuracy and robustness. Thus, the verified SFC method was successfully applied to quantification of flavonoids present in CRP samples. The results indicated that SFC has the potential to become an excellent alternative for the analysis of flavonoids in herbal medicines, owning to its intrinsic features like high efficiency, separation speed and eco-friendly characteristics.

16.
Commun Biol ; 2: 418, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31754648

RESUMO

Alpha-synuclein (α-syn) is an abundant neuroprotein elevated in cocaine addicts, linked to drug craving, and recruited to axon terminals undergoing glutamatergic plasticity - a proposed mechanism for substance abuse. However, little is known about normal α-syn function or how it contributes to substance abuse. We show that α-syn is critical for preference of hedonic stimuli and the cognitive flexibility needed to change behavioral strategies, functions that are altered with substance abuse. Electron microscopic analysis reveals changes in α-syn targeting of ventral tegmental area axon terminals that is dependent upon the duration of cocaine exposure. The dynamic changes in presynaptic α-syn position it to control neurotransmission and fine-tune the complex afferent inputs to dopamine neurons, potentially altering functional dopamine output. Cocaine also increases postsynaptic α-syn where it is needed for normal ALIX function, multivesicular body formation, and cocaine-induced exosome release indicating potentially similar α-syn actions for vesicle release pre- and post-synaptically.

17.
J Pineal Res ; : e12621, 2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31714635

RESUMO

Melatonin (MT) regulates reproductive performance as a potent antioxidant; however, its beneficial effects on oocyte development remain largely unknown, especially in human oocytes. The collected 193 immature oocytes from the controlled ovarian hyperstimulation (COH) cycle underwent in vitro maturation (IVM) in IVM medium contained 10 µmol/L MT (n = 105, M group) and no MT (n = 88, NM group), followed by insemination and embryo culture. The results showed that the high-quality blastocyst formation rate in the M group (28.4%) was significantly higher than that in the NM group (2.0%) (P = .0001), and the aneuploidy rate was low (15.8%). In the subsequent clinical trials, three healthy infants were delivered. Next, single-cell RNA-seq data revealed 1026 differentially expressed genes (DEGs) between the two groups, KEGG enrichment analysis revealed that the majority of DEGs involved in oxidative phosphorylation pathway, which associated with ATP generation, was upregulated in the M group. Finally, confocal fluorescence staining results revealed that the mitochondrial membrane potential in the oocytes significantly increased and intracellular ROS and Ca2+ levels greatly decreased in the M group. Melatonin can promote the development of immature human oocytes retrieved from the COH cycle into healthy offspring by protecting mitochondrial function.

18.
Mol Cell Probes ; 48: 101469, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31629029

RESUMO

Diabetic encephalopathy is a severe diabetic complication characterized by cognitive dysfunction and neuropsychiatric disability. Methylglyoxal (MGO), a highly reactive metabolite of hyperglycemia, serves as a major precursor of advanced glycation end products that play key roles in diabetic complications. Ginsenoside Rb1 (abbreviated as Rb1) has received extensive attention due to its potential therapeutic effects on diabetes and neurodegeneration. Therefore, this study aimed to investigate the effects of Rb1 on MGO-induced damage in SH-SY5Y cells and the related mechanism. SH-SY5Y cells were pretreated with Rb1 for 8 h and then exposed to MGO (0.5 mM) for 24 h. Cell survival was assessed by the MTT assay. Cell apoptosis was assessed using Hoechst 33342/propidium iodide (PI) staining and an Annexin-V/PI kit. The activities of oxidative stress markers were examined using commercial kits. Reactive oxygen species (ROS) staining and JC-1 staining were used to evaluate mitochondria injury. In addition, protein levels were measured by Western blot analysis. As a result, Rb1 alleviated the injury induced by MGO by increasing the activities of superoxide dismutase, catalase and total glutathione, decreasing the level of malondialdehyde, and alleviating mitochondrial damage and ROS production. Furthermore, Rb1 could enhance the Bcl-2/Bax ratio, inhibit the expression of cleaved caspase-3 and cleaved caspase-9, and enhance the levels of phosphorylated Akt. Moreover, the protective effects of Rb1 against MGO-induced apoptosis were partly abolished by LY294002, a specific inhibitor of phosphatidylinositol 3-kinase (PI3K) phosphorylation. Our results demonstrated that Rb1 ameliorated MGO-induced oxidative stress and apoptosis in SH-SY5Y cells via activating the PI3K/Akt signaling pathway.

19.
Reprod Biol Endocrinol ; 17(1): 78, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31615517

RESUMO

BACKGROUND: Immune dysregulation is one of the mechanisms to promote endometriosis (EMS). Various T cell subpopulations have been reported to play different roles in the development of EMS. The mucosa-associated invariant T cell (MAIT) is an important T cell subset in the pathogenesis of various autoimmune diseases. Evidence has indicated that there are three functionally distinct MAIT subsets: CD4+, CD8+ and CD4/CD8-/- (double negative, DN) MAIT cells. Till now, the associations between endometriosis and MAIT have not been studied. Our research investigates different MAIT subpopulations in peripheral blood (PB) and peritoneal fluid (PF) from EMS patients. METHODS: Thirty-two EMS patients and eighteen controls were included. PB and PF were collected. Tests of cytokines in plasma and PF were performed by ELISA kit. Characterisations of MAIT were done by flow cytometry. MAIT cells have been defined as CD3 + CD161 + Vα7.2+ cells. Based on CD4 and CD8 expression, they were divided into CD8+MAIT, CD4+MAIT and DN MAIT. RESULTS: Enrichments of MAIT cells, especially CD4 and CD8 MAIT subsets were found. Moreover, CD8 MAIT cells had a high activation in the EMS group. EMS patients produced higher level of IL-8/12/17 as compared to these from controls. On the contrary, control patients exhibited an impressive upregulation of DN MAIT cells, however, these DN MAIT cells from controls showed a higher expression of PD-1. Lastly, we performed the relevance analysis, and discovered that the accumulation of PB MAIT cells positively correlated with an elevated level of serum CA125 production in EMS group. CONCLUSION: These results suggest that different MAIT subsets play distinct roles in the progression of endometriosis.

20.
Exp Ther Med ; 18(5): 4173, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31641389

RESUMO

[This corrects the article DOI: 10.3892/etm.2018.6721.].

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