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1.
PLoS One ; 15(3): e0230381, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32176727

RESUMO

Visceral leishmaniasis (VL) is the most fatal form of leishmaniasis if left untreated and 50,000 to 90,000 new cases of VL occur worldwide each year. Although various vaccines had been studied in animal models, none of them was eligible to prevent human from infections. In this study, according to the silico analysis of Leishmania Amastin, Kmp-11 and Gp63 protein, dominant epitope sequences of these proteins were selected and linked to construct dominant multi-epitopes DNA and protein vaccines (Amastin-Kmp-11, Amastin-Gp63 and Kmp-11-Gp63) against VL. BALB/c mice were immunized with a DNA prime-protein boost immunization strategy and challenged with a new Leishmania parasite strain isolated from a VL patient. After immunization, the results including specific antibody titers, IL-4 and TNF-α levels, and CD4 and CD8 T cell proportion suggested the potent immunogenicity of the three vaccines. After infection, the results of spleen parasite burdens in the three vaccine groups were significantly lower than those of control groups, and the parasite reduction rates of Amastin-Kmp-11, Amastin-Gp63 and Kmp-11-Gp63 groups were 89.38%, 91.01% and 88.42%, respectively. Spleen smear observation and liver histopathological changes showed that all vaccine groups could produce significant immunoprotection against VL and Amastin-Gp63 vaccine was the best. In conclusion, our work demonstrated that the three dominant multi-epitopes Amastin-Kmp-11, Amastin-Gp63 and Kmp-11-Gp63 DNA prime-protein boost vaccines might be new vaccine candidates for VL, and the Amastin-Gp63 vaccine have best efficacy.

2.
Breastfeed Med ; 2020 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-32129666

RESUMO

Background: To evaluate the efficacy of a multidisciplinary quality improvement intervention to promote mother's own milk feeding and reduce necrotizing enterocolitis (NEC) in very low-birth-weight infants. Materials and Methods: We conducted a pre (January 2014 to March 2015)-post (April 2015 to June 2016), nonrandomized, interventional cohort study of infants born at <1,500 g birth weight and admitted to the Fudan University Children's Hospital level III neonatal intensive care unit in Shanghai. The intervention included establishing a breast milk promotion team and breast milk pumping room, educating staff and parents, and distributing teaching materials. The primary outcome was breast milk feeding rate. Secondary outcomes included incidences of NEC, NEC needing surgery, mortality, and time to full enteral feeds. Results: A total of 488 infants (210 baseline, 278 intervention) <1,500 g were enrolled. The intervention group had significantly increased feeding rates for any mother's milk (34.76% vs. 80.58%; p < 0.01) and high-volume mother's milk (≥50% of feeds; 22.86% vs. 61.15%; p < 0.01), and decreased incidence of NEC needing surgery (7.62% vs. 3.24%; adjusted odds ratio [OR] 0.32, 95% confidence interval [CI] 0.14-0.76). There were no significant differences in rates of mortality (0.5% vs. 1.49%; adjusted OR 2.10, 95% CI 0.22-19.6), NEC (10.00% vs. 7.55%; adjusted OR 0.59, 95% CI 0.31-1.14), and time to full enteral feeds (20.18 ± 1.67 days vs. 24.15 ± 1.65 days; adjusted OR = 1.09, 95% CI 0.99-1.21). Conclusions: Our quality improvement initiative increased the consumption of mother's own milk and reduced the severity of NEC in very low-birth-weight infants.

4.
Thorac Cancer ; 2020 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-32130756

RESUMO

BACKGROUND: We aimed to obtain a set of health state utility scores of patients with esophageal cancer (EC) and precancerous lesions in China, and to explore the influencing factors of health-related quality of life (HRQoL). METHODS: A hospital-based multicenter cross-sectional study was conducted. From 2013 to 2014, patients with EC or precancerous lesions were enrolled. HRQoL was assessed using a European quality of life-5 dimension (EQ-5D-3L) instrument. Multivariable linear regression analysis was performed to explore the influencing factors of the EQ-5D utility scores. RESULTS: A total of 2090 EC patients and 156 precancer patients were included in the study. The dimension of pain/discomfort had the highest rate of self-reported problems, 60.5% in EC and 51.3% in precancer patients. The mean visual analog scale (VAS) score for EC and precancer patients were 68.4 ± 0.7 and 64.5 ± 3.1, respectively. The EQ-5D utility scores for EC and precancer patients were estimated as 0.748 ± 0.009 and 0.852 ± 0.022, and the scores of EC at stage I, stage II, stage III, and stage IV were 0.693 ± 0.031, 0.747 ± 0.014, 0.762 ± 0.015, and 0.750 ± 0.023, respectively. According to the multivariable analyses, the factors of region, occupation, household income in 2012, health care insurance type, pathological type, type of therapy, and time points of the survey were statistically associated with the EQ-5D utility scores of EC patients. CONCLUSIONS: There were remarkable decrements of utility scores among esophageal cancer patients, compared with precancer patients. The specific utility scores of EC would support further cost-utility analysis in populations in China.

5.
Nanotechnology ; 2020 Mar 13.
Artigo em Inglês | MEDLINE | ID: mdl-32168494

RESUMO

With the increasing global energy demand, traditional energy sources are gradually failing to meet society's needs while also having a potential of being harmful to the environment. As such, energy generating technologies capable of converting ubiquitous environmental energy into usable forms, such as electricity, have received increasing attention. In this research, a power generating device composed of a graphene (G) and titanium dioxide nanowire (TiO2 NWs) double-layer structure is prepared by an electrophoretic deposition method. Since both materials have special nanochannel structures and non-zero zeta potential, they can convert environmental energy into electricity through the diffusion, ionization, and natural evaporation of water. Furthermore, the efficiency of this novel sensor is much higher than their respective single-layer devices. By application of only 6 µL of water, the open circuit voltage (UOC) generated on the G-TiO2 sensor is as high as 1.067 ± (0.008) V. In comparison, TiO2 NWs single-layer can only generate a UOC around 500 mV, and graphene itself can only produce a UOC no more than 250 mV under a same condition. Additionally, the effect of different deposition times of graphene on the surface morphology and thickness of graphene film is explored, and the effects of these changes in microstructure on performance is discussed in depth. Aside from power generation, the high sensitivity of the device to different volumes of water brings its use in the detection of trace amounts of water, and its high efficiency energy conversion suggests a potential application as a power supply. This research not only provides a satisfactory candidate for inexpensive and efficient evaporative power generation, but also builds a foundation for developing new, intelligent, and self-powered electronic technologies.

6.
Foodborne Pathog Dis ; 2020 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-32186920

RESUMO

Some plant essential oils were reported to have antimicrobial activity and have the potential to replace chemical preservatives in food industry. In this study, the antibacterial activity and possible mechanism of Perilla frutescens essential oil (PEO) were evaluated using Enterococcus faecalis R612-Z1 as the target strain. The minimum inhibition concentration of PEO against E. faecalis was 0.5 µL/mL. The PEO solutions at the concentrations higher than minimum inhibition concentration had varying degrees of bactericidal effects against E. faecalis. With the addition of PEO, the cell membrane integrity was destroyed, the cell membrane potential was decreased, and the intracellular adenosine triphosphate loss was increased. By testing the bacterial counts and total volatile basic nitrogen contents in chicken breast meat, PEO can significantly inhibit the growth of E. faecalis. The results showed that PEO can be used as an effective natural food preservative during food storage.

7.
Artigo em Inglês | MEDLINE | ID: mdl-32204408

RESUMO

Under the rapid development of urbanization, fire service becomes one of the biggest contributive factors to personal health and property safety. A reasonable plan of fire services should first address the issue of service area delimitation for fire emergency facilities. Specifically, there are two key factors for fire services including rescue efficiency and load balancing, which are usually handled by the space partitioning methods (e.g., Voronoi diagram). The traditional methods tend to model the space in a homogeneous plane with Euclidean distance, while in reality, the movement of rescuing is constrained by the street network. In addition, the built environment is complex by its variation of fire risk across places. Therefore, we propose a novel constrained Voronoi diagram for fire service area delimitation by adding the datasets of street network and historical fire incidents. Considering the prior knowledge that a fire engine is expected to reach the location of incident within five minutes, which is also called Golden 5 min, we propose a network partitioning algorithm which is able to increase the five-minute coverage of fire stations. Through a case study in Nanjing, China, we demonstrate the practicability of the proposed method in delimitating service areas of fire stations across time.

8.
Proc Natl Acad Sci U S A ; 117(11): 6086-6091, 2020 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-32123080

RESUMO

Recombinant immunotoxins (RITs) are chimeric proteins composed of an Fv and a protein toxin being developed for cancer treatment. The Fv brings the toxin to the cancer cell, but most of the RITs do not reach the tumor and are removed by other organs. To identify cells responsible for RIT removal, and the pathway by which RITs reach these cells, we studied SS1P, a 63-kDa RIT that targets mesothelin-expressing tumors and has a short serum half-life. The major organs that remove RIT were identified by live mouse imaging of RIT labeled with FNIR-Z-759. Cells responsible for SS1P removal were identified by immunohistochemistry and intravital two-photon microscopy of kidneys of rats. The primary organ of SS1P removal is kidney followed by liver. In the kidney, SS1P passes through the glomerulus, is taken up by proximal tubular cells, and transferred to lysosomes. In the liver, macrophages are involved in removal. The short half-life of SS1P is due to its very rapid filtration by the kidney followed by degradation in proximal tubular cells of the kidney. In mice treated with SS1P, proximal tubular cells are damaged and albumin in the urine is increased. SS1P uptake by kidney is reduced by coadministration of l-lysine. Our data suggests that l-lysine administration to humans might prevent SS1P-mediated kidney damage, reduce albumin loss in urine, and alleviate capillary leak syndrome.

9.
Sleep Med ; 70: 17-26, 2020 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-32179428

RESUMO

IMPORTANCE: Although several strategies using transcranial direct current stimulation (tDCS) have been investigated to treat major depressive disorder (MDD), the efficacy of this treatment for patients with MDD who also have insomnia is unclear. OBJECTIVE: To observe the effects of tDCS on sleep quality and depressive symptoms in patients with MDD who have insomnia. METHODS: We conducted a randomized, double-blinded study involving adults with major depression and insomnia. We randomly assigned patients to either add tDCS or to sham tDCS to their regular treatment. After randomization, we treated a total of 90 patients at the Kangning Hospital, Ningbo, China. We allocated 47 patients to the tDCS group and 43 to the sham tDCS group. The tDCS treatment procedure included 20 sessions of 2-mA stimulation of the dorsolateral prefrontal cortex (DLPFC) for 30 min, which was followed by four weekly treatments. The anode and cathode electrodes were placed on the left and right DLPFC, respectively. We recorded the Self-rating Depression Scale (SDS), Self-rating Anxiety Scale (SAS), Pittsburgh Sleep Quality Inventory (PSQI), and Polysomnography (PSG) at Day 1 and Day 28. RESULTS: Compared with the sham tDCS group, the active tDCS group showed improved total scores of SAS and SDS. PSQI total score and all PSQI sub-divisions, except for "sleep duration and sleep efficiency," significantly improved after treatment. We also observed that tDCS affected sleep architecture, by increasing total sleep time and improving sleep efficiency through PSG. CONCLUSIONS: Our study demonstrated the effect of tDCS on sleep quality and depressive symptoms in patients with MDD and insomnia. These results suggested that tDCS stimulation not only improved symptoms of depression and anxiety but also had a positive effect on sleep quality in patients with MDD. For patients with depression and insomnia, tDCS stimulation could be a good supplement to drugs.

10.
Lancet Child Adolesc Health ; 4(4): 256-257, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32119842
11.
Ophthalmic Res ; 2020 Mar 16.
Artigo em Inglês | MEDLINE | ID: mdl-32172234

RESUMO

PURPOSE: To investigate if optical quality assessed by Optical Quality Analysis System(OQAS)is a new factor for psychological disturbance and vision related quality of life(QoL) in Chinese patients with primary open angle glaucoma (POAG). METHODS: Cross-sectional study. OQAS, automated static perimetry, the Hospital Anxiety and Depression Scale (HADS) Questionnaire and the 25-item National Eye Institute Visual Function Questionnaire (NEI VFQ-25) were used to assess optical quality, vision field, psychological health and QoL in 64 POAG patients. Correlation between the OQAS parameters and QoL or psychological health was analyzed by linear regression model. RESULTS: OQAS parameters, the modulation transfer function cut off frequency (MTF cutoff) and the mean OQAS values (mean OV), were significantly related to mean deviation (MD). Statistically significant correlation was also found between MTF cutoff, the mean OV and QoL in more than half of the NEI VFQ-25 subscale scores and the composite. The correlation coefficients between OQAS parameters and QoL were similar to those between MD and QoL in most of the subscale scores. HADS-Depression and HADS-Anxiety scores significantly correlated with MD in the worse eye, the composite and 3subscales of NEI VFQ-25, general health, social function and mental health, but uncorrelated with optical quality parameters measured by OQAS. CONCLUSIONS: OQAS assessed optic quality is negatively related to MD and is a valid indicator for vision related QoL, but not for psychological health in our study group. OQAS might be an alternative or supplement to perimeter in POAG patients.

12.
Water Res ; 175: 115656, 2020 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-32145399

RESUMO

Microalgae-mediated biodegradation of antibiotics has recently gained increased attention from international scientific community. However, limited information is available regarding microalgae-mediated biodegradation of SMX in a co-metabolic system. Here we investigated the biodegradation of sulfamethoxazole (SMX) by five algal species (Pseudokirchneriella subcapitata, Scenedesmus quadricauda, Scenedesmus obliquus, Scenedesmus acuminatus and Chlorella pyrenoidosa), and its transformation pathways by C. pyrenoidosa in a sodium acetate (3 mM) co-metabolic system. The results showed that the highest SMX dissipation (14.9%) was detected by C. pyrenoidosa after 11 days of cultivation among the five tested algal species in the absence of other carbon sources. The addition of sodium acetate (0-8 mM) significantly enhanced the dissipation efficiency of SMX (0.4 µM) from 6.05% to 99.3% by C. pyrenoidosa after 5 days of cultivation, and the dissipation of SMX followed the first-order kinetic model with apparent rate constants (k) ranging from 0.0107 to 0.9811 d-1. Based on the results of mass balance analysis, biodegradation by C. pyrenoidosa was the main mechanism for the dissipation of SMX in the culture medium. Fifteen phase I and phase II metabolites were identified, and subsequently the transformation pathway was proposed, including oxidation, hydroxylation, formylation and side chain breakdown, as well as pterin-related conjugation. The majority of metabolites of SMX were only observed in the culture medium and varied with cultivation time. The findings of the present study showed effective co-metabolism of a sulfonamide by microalgae, and it may be applied in the aquatic environment remediation and wastewater treatment in the future.

13.
Proc Natl Acad Sci U S A ; 117(8): 4328-4336, 2020 Feb 25.
Artigo em Inglês | MEDLINE | ID: mdl-32029582

RESUMO

Epigenetic alterations and metabolic dysfunction are two hallmarks of aging. However, the mechanism of how their interaction regulates aging, particularly in mammals, remains largely unknown. Here we show ELOVL fatty acid elongase 2 (Elovl2), a gene whose epigenetic alterations are most highly correlated with age prediction, contributes to aging by regulating lipid metabolism. Impaired Elovl2 function disturbs lipid synthesis with increased endoplasmic reticulum stress and mitochondrial dysfunction, leading to key accelerated aging phenotypes. Restoration of mitochondrial activity can rescue age-related macular degeneration (AMD) phenotypes induced by Elovl2 deficiency in human retinal pigmental epithelial (RPE) cells. We revealed an epigenetic-metabolism axis contributing to aging and potentially to antiaging therapy.

14.
Fish Shellfish Immunol ; 99: 184-189, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32035168

RESUMO

The intestine is the primary target of pathogenic microbes during invasion. However, the interaction of Vibrio parahaemolyticus (V. parahaemolyticus) with intestinal epithelial cells and its effects on the intestinal function of Litopenaeus vannamei (L. vannamei) are poorly studied. Therefore, the aim of this study was to investigate the influence of V. parahaemolyticus infection on intestinal barrier function and nutrient absorption in L. vannamei. In the present study, a total of 90 shrimp were randomly divided into two groups including the control group and V. parahaemolyticus infection group (final concentration of 1 × 105 CFU/mL), with three replicates per group. The result showed that compared with the control group, V. parahaemolyticus infection increased (P < 0.05) serum diamine oxidase activity and endotoxin quantification, and down-regulated (P < 0.05) the mRNA levels of intestinal peroxinectin, integrin, midline fasciclin at 48 h and 72 h; V. parahaemolyticus infection decreased (P < 0.05) the mRNA expression of intestinal amino acid transporter (CAT1, EAAT3 and ASCT1) and glucose transporter (SGLT-1, GLUT) at 24 h, 48 h and 72 h, and increased (P < 0.05) serum glucose and amino acid (Asp, Thr, Ser, Glu, Gly, Ala, Val, Ile, Leu, Tyr, Phe, Lys, His and Arg) concentration at 24 h. The results indicated that V. parahaemolyticus infection increased intestinal permeability, inhibited absorption of glucose and amino acid in L. vannamei.

15.
J Environ Sci (China) ; 90: 385-394, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32081334

RESUMO

Nanometer-size zero-valent iron (NZVI) is an efficient reducing agent, but its surface is easily passivated with an oxide layer, leading to reaction inefficiency. In our study, oxalate (OA) was introduced into this heterogeneous system of NZVI, which could form ferrioxalate complexes with the NZVI surface-bound Fe3+ and dissolved Fe3+ in the solution. Photolysis of ferrioxalate complexes can facilitate the generation of Fe2+ from Fe3+ and CO2•- radical, both species have strong reduction capacity. Hence, a "photo-oxalate-Fe(0)" system through sunlight induction was established, which not only prohibited the formation of a surface passivation layer, but also displayed a synergetic mechanism of ferrioxalate photolysis to enhance reduction, exhibiting remarkably higher degradation activity (several times faster) toward the model pollutant Cr(VI) than the mechanism with NZVI alone. Factor tests suggested that both NZVI dosage and OA content markedly affected the reduction rate. Low pH was beneficial to the reduction efficiency. Moreover, recyclability experiment showed that the reduction rate decreased from 0.21706 to 0.03977 min-1 after three cycles of reuse due to the NZVI losing reaction activity generally, but the system still maintained considerable reduction capacity. Finally, a mechanism was revealed whereby NZVI would transform to Fe oxides after the exhaustion of its reductive power, and the photolysis of ferrioxalate to promote the cycling of iron species played the predominant role in providing extra reduction ability. These features confirm that introduction of OA into Cr(VI) reduction by NZVI through sunlight induction is advantageous and promising.

16.
Dalton Trans ; 2020 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-32065177

RESUMO

Preparing luminescence-stable lanthanide materials in a facile manner has always been an intriguing challenge. Herein we report an easy approach to the preparation of a heat-set lanthanide-based metallogel (H6L/Tb gel) with stable luminescence from 5,5',5''-(1,3,5-triazine-2,4,6-triyl)tris(azanediyl) triisophthalate (H6L) as a Tb3+ coordinating ligand. The heat-set H6L/Tb gel exhibits luminescence that mostly retains its intensity in the 0-90 °C temperature range and under mechanical stimulus. In addition, the H6L/Tb xerogel is also luminescent in aqueous solution. UV-vis and FT-IR spectroscopy and XRD studies reveal that the stable luminescence of the H6L/Tb gel depends on heat-set-induced strong hydrogen bonding, π-π stacking, and metal-ligand interactions. This study provides a heat-set approach to the preparation of luminescence-stable metallogel materials, such metallogels are potentially useful in anti-counterfeiting and sensing fields, among others.

18.
Chemosphere ; 248: 126102, 2020 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-32045978

RESUMO

A novel adsorbent, the uniform fiber ball (UFB) loaded with polypyrrole (UFB-PPy), was synthesized for Cr(VI) removal from water in this paper. The structure of the UFB and UFB-PPy were characterized by SEM, EDS, FT-IR, BET, XPS and TG. The adsorption properties of UFB-PPy towards Cr(VI) were investigated by the effects of temperature, initial concentration of Cr(VI), interfering ions and contact time in batch experiments, the isothermal models (Langmuir, Freundlich and Temkin) and the kinetic models (Pseudo first-order kinetic, Pseudo second-order kinetic and Intra-particle diffusion models) were used to describe the adsorption behavior. The effects of the initial concentration and flow rate of the Cr(VI) solution in the column experiments were also studied, and the dynamic models (Yoon-Nelson, Adams-Bohart and Wolborska model) were applied to predict the adsorption performance. The Cr(VI) removal mechanism of UFB-PPy was revealed by studying the effect of pH on adsorption, testing of Cl-, and analyzing the XPS. The results showed that UFB-PPy exhibited excellent adsorption properties for Cr(VI) both in batch and column adsorption. The possible adsorption mechanism involved electrostatic attraction, ion exchange and reduction. Conveniently, the chromium resources can be recovered with the form of high-purity Cr2O3 by simple calcination of Cr(VI)-captured UFB-PPy (UFB-PPy-Cr).

19.
Trials ; 21(1): 170, 2020 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-32046760

RESUMO

BACKGROUND: Necrotizing enterocolitis (NEC) is the leading cause of death among preterm infants born at < 30 weeks' gestation. The incidence of NEC is reduced when infants are fed human milk. However, in many neonatal intensive care units (NICUs), it is standard practice to freeze and/or pasteurize human milk, which deactivates bioactive components that may offer additional protective benefits. Indeed, our pilot study showed that one feed of fresh mother's own milk per day was safe, feasible, and can reduce morbidity in preterm infants. To further evaluate the benefits of fresh human milk in the NICU, a randomized controlled trial is needed. METHODS: Our prospective multicenter, double-blinded, randomized, controlled trial will include infants born at < 30 weeks' gestation and admitted to one of 29 tertiary NICUs in China. Infants in the intervention (fresh human milk) group (n = 1549) will receive at least two feeds of fresh human milk (i.e., within 4 h of expression) per day from the time of enrollment until 32 weeks' corrected age or discharge to home. Infants in the control group (n = 1549) will receive previously frozen human milk following the current standard protocols. Following informed consent, enrolled infants will be randomly allocated to the control or fresh human milk groups. The primary outcome is the composite outcome mortality or NEC ≥ stage 2 at 32 weeks' corrected age, and the secondary outcomes are mortality, NEC ≥ stage 2, NEC needing surgery, late-onset sepsis, retinopathy of prematurity (ROP), bronchopulmonary dysplasia (BPD), weight gain, change in weight, increase in length, increase in head circumference, time to full enteral feeds, and finally, the number and type of critical incident reports, including feeding errors. DISCUSSION: Our double-blinded, randomized, controlled trial aims to examine whether fresh human milk can improve infant outcomes. The results of this study will impact both Chinese and international medical practice and feeding policy for preterm infants. In addition, data from our study will inform changes in health policy in NICUs across China, such that mothers are encouraged to enter the NICU and express fresh milk for their infants. TRIAL REGISTRATION: Chinese Clinical Trial Registry; #ChiCTR1900020577; registered January 1, 2019; http://www.chictr.org.cn/showprojen.aspx?proj=34276.

20.
Aging (Albany NY) ; 12(4): 3249-3265, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-32065780

RESUMO

OBJECTIVE: The high mortality rate associated with acute respiratory distress syndrome (ARDS) is a major challenge for intensive care units. In the present study, we applied bioinformatics and animal models to identify core genes and potential corresponding pathways in ARDS. RESULTS: Using bioinformatics analysis, IL-1ß was identified as the core gene of ARDS. Cell experiments showed that up-regulation of IL-1ß downregulates claudin18 to promote lung barrier function damage by regulating the IL-1ß-HER2/HER3 axis, further promoting the development of ARDS. This was validated in the animal models. CONCLUSION: IL-1ß promotes the development of ARDS by regulating the IL-1ß-HER2/HER3 axis. These findings deepen the understanding of the pathological mechanisms of ARDS. METHODS: Transcription data sets related to ARDS were subjected to differential expression gene analysis, functional enrichment analysis, and receiver operating characteristic curve analysis and, so as to identify core genes in ARDS. Cell experiments were used to further explore the effects of core genes on lung barrier function damage. Animal models were applied to validate the effects of core gene in mediating biological signal pathways in ARDS.

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