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1.
Artigo em Inglês | MEDLINE | ID: mdl-33624238

RESUMO

Most studies focused on the temporal trend of mortality risk associated with temperature exposure. The relative role of heat, cold, and temperature variation (TV) on morbidity and its temporal trends are explored insufficiently. This study aims to investigate the temporal trends of emergency ambulance dispatch (EAD) risk and the attributable burden of heat, cold, and hourly temperature variation (HTV). We collected time-series data of daily EAD and ambient temperature in Shenzhen from 2010 to 2017. HTV was calculated as the standard deviation of the hourly temperatures between 2 consecutive days. Quasi-Poisson generalized additive models (GAM) with a time-varying distributed lag nonlinear model (DLNM) were applied to examine temporal trends of the HTV-, heat-, and cold-EAD association. The temporal variation of the attributable fraction (AF%) and attributable number (AN) for different temperature exposures was also calculated. The largest RR was observed in extreme cold [1.30 (95% CI: 1.18, 1.43)] and moderate cold [1.25 (95% CI: 1.17, 1.34)]. Significant increasing trends in HTV-related effects and burden were observed, especially for the extreme HTV effects (P for interaction < 0.05). Decreasing trends were observed in the heat-related effect and burden, though it showed no significance (P for interaction = 0.46). There was no clear change pattern of cold-related effects and burdens. Overall, the three temperature exposure caused 13.7% of EAD, of which 4.1%, 4.3%, and 5.3% were attributed to HTV, heat, and cold, respectively. All the temperature indexes in this study, especially the cold effect, are responsible for the increased risk of EAD. People have become more susceptible to HTV over the recent decade. However, there is no clear evidence to support the temporal change of the population's susceptibility to heat and cold. Thus, in addition to heat and cold, the emergency ambulance service department should pay more attention to HTV under climate change.

2.
Technol Cancer Res Treat ; 20: 1533033821990046, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33517857

RESUMO

BACKGROUND: Cancer is associated with genetic variants of DNA repair genes that alter DNA repair capacity. The aim of this meta-analysis was to evaluate the relations between the rs13181 and rs1799793 XPD gene polymorphisms and risk for hepatocellular carcinoma (HCC) and gastric cancer. METHODS: Relevant publications were systematically sought from Web of Science, Pubmed, and China Academic Journals Full-text Database. The selection of eligible studies was performed by 2 independent authors. A total of 32 case-control studies were included. Meta-analyses were undertaken in all study participants and each ethnic group. RESULTS: The risk of HCC was significantly increased with the XPD rs13181 G allele (P = 0.028, pooled odds ratio (OR) = 1.36, 95% confidence interval (CI) = 1.03-1.80) in all study participants. A subgroup analysis by ethnicity showed that the association was significant in Chinese (P = 0.009, pooled OR = 1.49, 95% CI = 1.11-2.02), but not in Caucasians (P = 0.619, pooled OR = 1.17, 95% CI = 0.64-2.13). Meta-analysis of the XPD rs1799793 polymorphism and HCC showed an association between its variant T allele and increased HCC risk in all study participants (P = 0.017, pooled OR = 1.23, 95% CI = 1.04-1.46, all Chinese). Our results showed no associations between the XPD rs13181 G allele and rs1799793 T allele and gastric cancer risk (rs13181: P = 0.298, pooled OR = 1.10, 95% CI = 0.92-1.31; rs1799793: P = 0.068, pooled OR = 1.31, 95% CI = 0.98-1.74). CONCLUSIONS: This meta-analysis demonstrated that the XPD rs13181 G allele and rs1799793 T allele have significant associations with HCC and may be risk factors for HCC in the Chinese population. Current evidence indicated that they are not related to gastric cancer risk.

3.
Curr Med Sci ; 41(1): 1-13, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33582899

RESUMO

Currently, little in-depth evidence is known about the application of extracorporeal membrane oxygenation (ECMO) therapy in coronavirus disease 2019 (COVID-19) patients. This retrospective multicenter cohort study included patients with COVID-19 at 7 designated hospitals in Wuhan, China. The patients were followed up until June 30, 2020. Univariate and multivariate logistic regression analyses were performed to identify the risk factors associated with unsuccessful ECMO weaning. Propensity score matching was used to match patients who received veno-venous ECMO with those who received invasive mechanical ventilation (IMV)-only therapy. Of 88 patients receiving ECMO therapy, 27 and 61 patients were and were not successfully weaned from ECMO, respectively. Additionally, 15, 15, and 65 patients were further weaned from IMV, discharged from hospital, or died during hospitalization, respectively. In the multivariate logistic regression analysis, a lymphocyte count ≤0.5×109/L and D-dimer concentration >4× the upper limit of normal level at ICU admission, a peak PaCO2 >60 mmHg at 24 h before ECMO initiation, and no tracheotomy performed during the ICU stay were independently associated with lower odds of ECMO weaning. In the propensity score-matched analysis, a mixed-effect Cox model detected a lower hazard ratio for 120-day all-cause mortality after ICU admission during hospitalization in the ECMO group. The presence of lymphocytopenia, higher D-dimer concentrations at ICU admission and hypercapnia before ECMO initiation could help to identify patients with a poor prognosis. Tracheotomy could facilitate weaning from ECMO. ECMO relative to IMV-only therapy was associated with improved outcomes in critically ill COVID-19 patients.


Assuntos
/terapia , Oxigenação por Membrana Extracorpórea/métodos , Adulto , Idoso , Estudos de Casos e Controles , China , Estado Terminal , Oxigenação por Membrana Extracorpórea/estatística & dados numéricos , Humanos , Masculino , Pessoa de Meia-Idade , Prognóstico , Pontuação de Propensão , Estudos Retrospectivos , Fatores de Risco , Análise de Sobrevida , Resultado do Tratamento , Adulto Jovem
4.
Cell Res ; 31(2): 126-140, 2021 02.
Artigo em Inglês | MEDLINE | ID: mdl-33420426

RESUMO

The current coronavirus disease 2019 (COVID-19) pandemic presents a global public health challenge. The viral pathogen responsible, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), binds to the host receptor ACE2 through its spike (S) glycoprotein, which mediates membrane fusion and viral entry. Although the role of ACE2 as a receptor for SARS-CoV-2 is clear, studies have shown that ACE2 expression is extremely low in various human tissues, especially in the respiratory tract. Thus, other host receptors and/or co-receptors that promote the entry of SARS-CoV-2 into cells of the respiratory system may exist. In this study, we found that the tyrosine-protein kinase receptor UFO (AXL) specifically interacts with the N-terminal domain of SARS-CoV-2 S. Using both a SARS-CoV-2 virus pseudotype and authentic SARS-CoV-2, we found that overexpression of AXL in HEK293T cells promotes SARS-CoV-2 entry as efficiently as overexpression of ACE2, while knocking out AXL significantly reduces SARS-CoV-2 infection in H1299 pulmonary cells and in human primary lung epithelial cells. Soluble human recombinant AXL blocks SARS-CoV-2 infection in cells expressing high levels of AXL. The AXL expression level is well correlated with SARS-CoV-2 S level in bronchoalveolar lavage fluid cells from COVID-19 patients. Taken together, our findings suggest that AXL is a novel candidate receptor for SARS-CoV-2 which may play an important role in promoting viral infection of the human respiratory system and indicate that it is a potential target for future clinical intervention strategies.


Assuntos
/metabolismo , Proteínas Proto-Oncogênicas/metabolismo , Receptores Proteína Tirosina Quinases/metabolismo , Mucosa Respiratória/citologia , Glicoproteína da Espícula de Coronavírus/metabolismo , Brônquios/citologia , Brônquios/metabolismo , Linhagem Celular , Humanos , Pulmão/citologia , Pulmão/metabolismo , Modelos Moleculares , Domínios e Motivos de Interação entre Proteínas , Proteínas Proto-Oncogênicas/análise , Receptores Proteína Tirosina Quinases/análise , Mucosa Respiratória/metabolismo , Glicoproteína da Espícula de Coronavírus/análise , Internalização do Vírus
5.
Nan Fang Yi Ke Da Xue Xue Bao ; 41(1): 69-74, 2021 Jan 30.
Artigo em Chinês | MEDLINE | ID: mdl-33509755

RESUMO

OBJECTIVE: To establish a mouse model bearing orthotopic temozolomide (TMZ)-resistant glioma that mimics the development of drug resistance in gliomas in vivo. METHODS: Seventy-eight adult C57BL/6 mice were randomly divided into 6 groups (n=13), including 3 TMZ induced groups with low, medium and high doses (5, 25, and 50 mg/kg, respectively) and 3 control groups. In each group, 5 mice were used for evaluating tumor size, 5 for observing survival, and 3 for collecting tumor tissues for primary cell culture. In low-dose TMZ induced group, 3 mice bearing orthotopic murine glioma GL261 cell xenografts received intraperitoneal injections of 5 mg/kg TMZ for 5 days followed by a 10-day washout period before collecting glioma tissues. Tumor cell suspensions were prepared and injected in the mice in the medium-dose group, which were treated with the same protocol but with an increased TMZ dose, and the tumor cells harvested from 3 mice were injected in the high-dose group. The mice bearing GL261 cell xenografts in the 3 control groups received no treatment or were injected with medium- or high-dose TMZ. Cell colony forming assay was used to assess TMZ resistance of each generation of the tumor cells; CCK8 assay was used to determine drug resistance index of the cells. RESULTS: The mouse models bearing TMZresistant glioma was successfully established. The cells from the high-dose induced group showed a significantly higher colony-forming rate than those from the high-dose control group (P < 0.05), and had a drug resistance 4.25 times higher than that of the cells from untreated control group. High-dose TMZ significantly reduced the tumor volume in the control group (P < 0.05) but not in the high-dose induced group (P < 0.01). The survival time of the tumor-bearing mice was significantly shortened in the high-dose induced group (P=0.0018). CONCLUSIONS: Progressive increase of TMZ doses in mice bearing orthotopic gliomas can effectively induce TMZ resistance of the gliomas.


Assuntos
Neoplasias Encefálicas , Glioma , Animais , Antineoplásicos Alquilantes/farmacologia , Neoplasias Encefálicas/tratamento farmacológico , Linhagem Celular Tumoral , Modelos Animais de Doenças , Resistencia a Medicamentos Antineoplásicos , Glioma/tratamento farmacológico , Camundongos , Camundongos Endogâmicos C57BL , Temozolomida/farmacologia , Temozolomida/uso terapêutico
6.
Artigo em Inglês | MEDLINE | ID: mdl-33428360

RESUMO

STUDY DESIGN: An anatomical study in fifteen cadavers. OBJECTIVE: To observe the anatomical relationship of the extrapedicular puncture approach with the spinal nerve and its branches, evaluate the risk of injury to these nerves and recommend a safe puncture path for lumbar extrapedicular percutaneous vertebroplasty (PVP) and percutaneous kyphoplasty (PKP). SUMMARY OF BACKGROUND DATA: There have been no reports on the anatomical relationship of the extrapedicular approach with the spinal nerve and its branches. METHODS: The positions, diameters and paths of the anterior ramus (AR), medial branch (MB), and lateral branch (LB) outside the pedicles of L1-L4 were observed, and the related anatomical parameters were measured. Three types of extrapedicular puncture paths were simulated: puncture inside the MB, puncture outside the MB, and puncture outside the LB. By evaluating the risk of injury to the MB, LB and AR, an appropriate puncture path was recommended. RESULTS: During puncture inside the MB, the incidence of MB injury was 93.3% to 100%. During puncture outside the MB, the incidence rates of MB injury at L1 and L2 were 10.0% and 3.3%, respectively, and the incidence of LB injury at L4 was 10.0%. During puncture outside the LB, there were no cases of injury to the LB or MB. There was no incidence of AR injury during L1-L3 extrapedicular puncture, but the risk of AR injury was 3.3% when the procedure was performed at L4. The recommended technique is puncture outside the LB, where the needle vertically crosses the transverse process, slides inward, and advances while being held tightly against the bone. CONCLUSIONS: Extrapedicular puncture occurs adjacent to the spinal nerve and its branches, posing a risk of injury to these nerves. At the L1-L3 levels, puncture outside the LB can avoid damage to the spinal nerve and its branches.Level of Evidence: N/A.

7.
Science ; 2021 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-33446483

RESUMO

The SREBP pathway controls cellular homeostasis of sterols. The key players in this pathway, Scap and Insig-1/2, are membrane-embedded sterol sensors. 25-hydroxycholesterol (25HC)-dependent association of Scap and Insigs acts as the master switch for the SREBP pathway. Here, we present cryo-EM analysis of the human Scap and Insig-2 complex in the presence of 25HC, with the transmembrane (TM) domains determined at an average resolution of 3.7 Å. The sterol sensing domain (SSD) in Scap and all six TMs in Insig-2 were resolved. A 25HC molecule is sandwiched between the S4-S6 segments in Scap and TMs 3/4 in Insig-2 in the luminal leaflet of the membrane. Unwinding of the middle of the Scap-S4 segment is crucial for 25HC binding and Insig association.

8.
Exp Mol Pathol ; 119: 104608, 2021 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-33503452

RESUMO

This study aimed to investigate the potential effects of miR-215, with exosomes as carriers, against skeletal muscle injury. Exosomes were isolated from rat bone marrow mesenchymal stem cells (rBMSCs) or rBMSCs overexpressing miR-215. Subsequently, rat myoblasts (L6) were treated with different exosomes and mimics, then exposed to H2O2. Cell viability and apoptosis were determined using the Cell Counting Kit-8 and Annexin V-FITC cell apoptosis assay kits, respectively. Reverse-transcriptase quantitative PCR (RT-qPCR) was used to examine the expression of related genes. Transmission electron microscopy, Nanosight, and western blotting showed that the exosomes were successfully isolated. PKH67 staining revealed that both exosomes and miR-215-modified exosomes were taken up by L6 cells. FABP3 was found to be the target gene of miR-215 via a dual luciferase reporter gene assay. In the L6 cells treated with H2O2, cell viability was significantly inhibited, whereas apoptosis significantly increased (P < 0.05). Exosomes significantly enhanced the viability of H2O2-induced cells and inhibited their apoptosis (P < 0.05). In addition, RT-qPCR showed that in the H2O2-induced L6 cells, FABP3, CDKN1A, and TP53 were significantly upregulated, while CCNB1 was significantly downregulated (P < 0.05). However, their expression levels were significantly reversed after treatment with miR-215-modified exosomes (P < 0.05). These findings indicate that the miR-215-modified exosomes may exert protective effects against skeletal muscle injury through the miR-215/FABP3 pathway and regulate the expression of CDKN1A, CCNB1, and TP53.

10.
J Phys Chem Lett ; : 861-868, 2021 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-33428415

RESUMO

The hot carrier cooling dynamics in the C-excitonic state of monolayer MoS2 is slowed down by the hot phonon bottleneck and Auger heating effects, as exploited by ultrafast transient absorption spectroscopy. The hot carrier cooling process, determined by the hot phonon bottleneck, can be prolonged through rising the excitation photon energy or increasing the absorbed photon flux. By inducing the Auger heating effect under higher absorbed photon flux, the hot carrier lifetime also increases at the low excitation photon energy. When these two effects are combined under higher excitation photon energy and higher absorbed photon flux, the hot phonon bottleneck is gradually weakened because of Auger recombination. In addition, the similar hot carrier phenomenon can be observed in A/B excitonic states owing to the same physical mechanism. Our work establishes a solid photophysics foundation for 2D transition-metal dichalcogenide applications in advanced energy conversion, optical quantum communication, quantum technology, etc.

11.
Support Care Cancer ; 2021 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-33403402

RESUMO

PURPOSE: To investigate the possible role of physical activity (PA) on sleep disturbance in breast cancer patients. METHODS: Literature in PubMed, Embase, and the Cochrane Library was systematically searched until January 30, 2020. Randomized controlled trials that focused on the role of PA interventions on sleep disturbance were selected. The main outcome measures included the global Pittsburgh Sleep Quality Index (PSQI) score and PSQI subscales. Subgroup analysis was performed based on the study area and intervention time. The stability and authenticity of the results were measured by sensitivity analysis and publication bias analysis, respectively. RESULTS: Six articles were included in this meta-analysis. There were no significant differences in global PSQI scores between the PA intervention group and the usual care group (P = 0.057). As for PSQI subscales, PA intervention could improve sleep quality (weighted mean difference = 0.22; 95% confidence interval 0.04-0.40; P = 0.018). There were no significant differences in sleep duration, sleep medication, sleep latency, habitual sleep efficiency, and daytime dysfunction between the two groups (all P > 0.05). CONCLUSION: PA serves as an effective intervention to improve sleep quality.

12.
ACS Sens ; 2021 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-33480688

RESUMO

Peroxynitrite (ONOO-) is an important reactive oxygen/nitrogen species that participates in a range of physiological and pathological processes by modulating ion flux through biological channels. Inspired by a ONOO--regulated K+ channel in vivo, herein, we describe the construction of a smart ONOO--driven nanosensor using a spirocyclic ring open/close reaction approach. The prepared nanosensor possessed a prominent ONOO- selectivity and sensitivity and rapid response (∼90 s) owing to the specific reaction between ONOO- and ligands on the nanosensor surface with a high ion rectification ratio (∼10) and ion gating ratio (∼4). Moreover, this nanosensor system also exhibits excellent stability and recyclability. Thus, these results will provide a new direction for the design of nanochannel-based sensors for future practical and biological applications.

13.
Cancer Lett ; 497: 229-242, 2021 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-33122099

RESUMO

Sonodynamic therapy (SDT) represents a promising modality that provides the possibility of non-invasively eliminating solid tumors in a site-directed manner. In light of the complexity and heterogeneity of tumors, more and more studies are attempting to combine SDT with other therapeutic methods so as to achieve better tumor treatment effect, which sheds new light on the potential of SDT-based synergistic therapeutics. Herein, the representative studies of SDT-instructed multimodal synergistic cancer therapy are comprehensively presented, such as sono-chemotherapy, sono-radiotherapy, sono-immunotherapy, and sono-chemodynamic therapy, etc., and their incorporate mechanisms are discussed in detail. The current challenges and future prospects to promote the advanced development of SDT-based nanomedicines in this burgeoning research field are highlighted. It is believed that such an emerging synergistic therapeutic modality based on SDT will play a more significant role in the field of tumor precision treatment medicine.

14.
Opt Express ; 29(1): 256-271, 2021 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-33362119

RESUMO

We theoretically investigate the preparation of mid-infrared (MIR) spectrally-uncorrelated biphotons from a spontaneous parametric down-conversion process using doped LN crystals, including MgO doped LN, ZnO doped LN, and In2O3 doped ZnLN with doping ratio from 0 to 7 mol%. The tilt angle of the phase-matching function and the corresponding poling period are calculated under type-II, type-I, and type-0 phase-matching conditions. We also calculate the thermal properties of the doped LN crystals and their performance in Hong-Ou-Mandel interference. It is found that the doping ratio has a substantial impact on the group-velocity-matching (GVM) wavelengths. Especially, the GVM2 wavelength of co-doped InZnLN crystal has a tunable range of 678.7 nm, which is much broader than the tunable range of less than 100 nm achieved by the conventional method of adjusting the temperature. It can be concluded that the doping ratio can be utilized as a degree of freedom to manipulate the biphoton state. The spectrally uncorrelated biphotons can be used to prepare pure single-photon source and entangled photon source, which may have promising applications for quantum-enhanced sensing, imaging, and communications at the MIR range.

15.
Artigo em Inglês | MEDLINE | ID: mdl-33307598

RESUMO

AIMS: The purpose of this study was to detect and compare the surface temperature of plantar vessels in mild DPN patients and healthy controls, to explore a simple, convenient and reliable method for early diagnosis of DPN, and to explore the influence of gender and age on vascular surface temperature. METHODS: In this study, 60 mild DPN patients (30 males and 30 females) were randomly recruited, and 60 healthy volunteers were recruited according to their age and gender. ICC3,1 was used to evaluate the repeatability of skin temperature measurement in vascular area. General linear model was used to analyze the difference of skin temperature between mild DPN patients and healthy controls. RESULTS: The infrared detection results of skin temperature corresponding to blood vessels showed excellent test-retest reliability. There was no significant difference in skin temperature between gender and age. Except for the posterior tibial artery, there were significant differences in skin temperature between mild DPN patients and healthy controls. CONCLUSIONS: For mild DPN patients, in the case of lower extremity artery corresponding skin temperature infrared detection does not indicate obvious abnormality, small vessels have appeared early abnormal body surface temperature, that is, the increase of skin temperature of related vessels. The conclusion of this study can provide an in vivo, noninvasive and convenient method for early diagnosis of DPN. This article is protected by copyright. All rights reserved.

16.
ACS Nano ; 2020 Dec 09.
Artigo em Inglês | MEDLINE | ID: mdl-33295753

RESUMO

Luminescence nanomaterial-based lateral flow assay (LFA) is promising for point-of-care tests. However, the detection sensitivity and accuracy are often affected by the interferences of autofluorescence and photon scattering from nitrocellulose membrane and colored plasma. Here, we describe a near-infrared to near-infrared upconversion nanoparticle (UCNP) immunolabeled LFA for background-free chromatographic detection of sepsis biomarker procalcitonin (PCT) in clinical human plasma. This upconversion immunolabeling enables both light excitation (at ∼980 nm) and anti-Stokes emission (at 800 nm) to be adopted within the first biological window (700-1000 nm), which eliminates background autofluorescence as well as photon scattering interferences, empowering a high-sensitivity detection without complicated procedures. After optimization, the described assay presented a limit of detection reaching down to 0.03 ng/mL, lower than the normal level (0.05 ng/mL), while having a detection range of 0.03-50 ng/mL that covers the clinical PCT level of interest (0.5-10 ng/mL). The assay recoveries in human serum samples were evaluated to be about 95-110%, whereas the inter- and intra-assay coefficient variations were both determined to be below 15%. Importantly, measured PCT concentrations in clinical samples are in good correlation with that of the electrochemiluminescence immunoassay (Roche) widely applied in large clinical settings. This near-infrared to near-infrared upconversion immunolabeling approach has direct implications for ultrasensitive and background-free point-of-care detection of other serum biomarkers in resource-limited clinical settings.

17.
J Infect Dev Ctries ; 14(11): 1264-1273, 2020 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-33296339

RESUMO

INTRODUCTION: To date, the relationship between the causative pathogens and the changes of hematological parameters was rarely referred and deserves further investigation. METHODOLOGY: A total of 825 adult patients, including 134 negative blood cultures patients and 691 bloodstream infection (BSI) patients, were screened for eligibility in this study. Receiver operating characteristic curves and binary logistic regression models were used to assess the power of hematological parameters to distinguish patients with BSI caused by different pathogens. RESULTS: Except for platelet-to-lymphocyte ratio (PLR) and platelet larger cell count (P-LCC), the other hematological parameters investigated in the study were significantly different in patients with BSI caused by different pathogens, including Candida. The specific combinations of lymphocyte count (LYM), platelet count (PLT), neutrophil-to-lymphocyte ratio (NLR), mean platelet volume (MPV), MPV-to-PLT ratio (MPV/PLT), platelet larger cell ratio (P-LCR), and C-reactive protein (CRP) can improve the ability to distinguish various BSI from negative blood cultures. The highest area under the curve of was 0.753 (95% CI 0.709-0.797) for positive blood cultures, 0.715 (95% CI 0.658-0.771) for Gram-positive pathogens BSI, 0.777 (95% CI 0.730-0.824) for Gram-negative pathogens BSI, 0.797 (95% CI 0.747-0.846) for Escherichia coli BSI, 0.943 (95% CI 0.899-0.987) for Enterobacter aerogenes BSI, 0.830 (95% CI 0.740-0.921) for Pseudomonas aeruginosa BSI, and 0.767 (95% CI 0.695-0.839) for Staphylococcus aureus BSI. CONCLUSIONS: The specific combinations of hematological parameters can improve the power to distinguish patients with BSI caused by different pathogens. Attention to these parameters can be easily integrated into daily medical activities, without extra costs.

18.
J Tradit Chin Med ; 40(6): 897-907, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33258340

RESUMO

OBJECTIVE: To evaluate the effectiveness and safety of a Shenlingbaizhu (SLBZ) formula in the treatment of irritable bowel syndrome (IBS). The effectiveness of SLBZ with or without conventional treatment was compared to that of conventional treatment alone. METHODS: A comprehensive literature search of four Chinese electronic databases, three English language databases, and two English language trial registries from inception to June 2019 was performed. Two authors independently screened the citations and retrieved full publications of randomized trials on the use of SLBZ with or without conventional treatment for IBS. The methodological quality of the trials was assessed with the Cochrane Collaboration's tool for assessing the risk of bias. Data were extracted and subjected to Meta-analysis to compare the efficacy of the SLBZ formula with or without conventional treatment to conventional treatment alone. RESULTS: Thirteen trials (comprising a total of 868 patients with IBS) were included in this review. The risk of bias of all 13 included trials was assessed as moderate. The SLBZ formula was associated with significant improvements in cure rate [relative risk (RR) score of 2.38, 95% confidence interval (CI) 1.43 to 3.95, I 2 = 0%; 8 trials, n = 487, fixed-effects model (FEM)], diarrhea severity score [mean difference (MD) score of -0.62, 95% CI -1.05 to -0.20, I 2 = 88%; 4 trials, n = 286, random effects model (REM)], abdominal pain severity score (MD score of -0.61, 95% CI -0.70 to -0.52, I 2 = 63%; 4 trials, n = 286, FEM), and abdominal distention severity score (MD score of -0.88, 95% CI -1.54 to -0.21, I 2 = 91%; 3 trials, n = 226, REM) compared to the conventional treatment alone. Adverse events were reported in five trials but only one of these indicated any adverse events associated with SLBZ. CONCLUSION: Based on the 13 trials reviewed here, the SLBZ formula with or without conventional treatment appeared to be safe and more effective in improving the cure rate and reducing the severity of diarrhea, abdominal pain, and abdominal distention compared to conventional treatment alone. However, these trials only generated a moderate quality of evidence, and well-designed and high-quality random controlled trials of the SLBZ formula for the treatment of IBS are required to confirm the efficacy of this treatment option.

19.
BMC Cancer ; 20(1): 1176, 2020 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-33261584

RESUMO

BACKGROUND: Hepatocellular carcinoma (HCC) is a common digestive tumor with great heterogeneity and different overall survival (OS) time, causing stern problems for selecting optimal treatment. Here we aim to establish a nomogram to predict the OS in HCC patients. METHODS: International Cancer Genome Consortium (ICGC) database was searched for the target information in our study. Lasso regression, univariate and multivariate cox analysis were applied during the analysis process. And a nomogram integrating model scoring and clinical characteristic was drawn. RESULTS: Six mRNAs were screened out by Lasso regression to make a model for predicting the OS of HCC patients. And this model was proved to be an independent prognostic model predicting OS in HCC patients. The area under the ROC curve (AUC) of this model was 0.803. TCGA database validated the significant value of this 6-mRNA model. Eventually a nomogram including 6-mRNA risk score, gender, age, tumor stage and prior malignancy was set up to predict the OS in HCC patients. CONCLUSIONS: We established an independent prognostic model of predicting OS for 1-3 years in HCC patients, which is available to all populations. And we developed a nomogram on the basis of this model, which could be of great help to precisely individual treatment measures.

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