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1.
Cerebrovasc Dis ; : 1-13, 2023 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-36634630

RESUMO

INTRODUCTION: Ischemic stroke (IS) is an extremely complex disease caused by the combined action of multiple environmental and genetic factors. CYP1B1 is a member of the cytochrome P450 protein family, and it is an important human drug-metabolizing enzymes. We aimed to explore the association between CYP1B1 genetic variants and IS risk in Chinese Han population. METHODS: We recruited 1,150 participants to conduct a "case-control" study. The assessment of association between candidate CYP1B1 genetic variants (rs2855658, rs10916, rs162560, rs2567206) and IS risk was performed by SNPStats online software. In addition, false-positive report probability analysis was used to detect whether the positive findings were just chance or noteworthy observations. Finally, the interaction of candidate SNPs in IS risk was evaluated by multifactor dimensionality reduction. RESULTS: The results showed that CYP1B1-rs2855658 was a risk factor for IS among ≥60-year-old (dominant: p = 0.034; overdominant: p = 0.026), smoking (heterozygote: p = 0.009; dominant: p = 0.004; overdominant: p = 0.012; log-additive: p = 0.003), and drinking participants (homozygous: p = 0.036; dominant: p = 0.019; recessive: p = 0.012; log-additive: p = 0.006). CYP1B1-rs10916 also was a risk factor for IS patients among ≥60-year-old (heterozygote: p = 0.047; overdominant: p = 0.048), smoking (dominant: p = 0.050; overdominant: p = 0.049), and drinking participants (dominant: p = 0.019; overdominant: p = 0.038; log-additive: p = 0.013). CONCLUSION: CYP1B1-rs10916 and CYP1B1-rs2855658 can increase the IS risk in Chinese Han population who are ≥60 years old, smoking, or drinking alcohol.

2.
World J Gastroenterol ; 29(2): 272-285, 2023 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-36687128

RESUMO

There is great heterogeneity among inflammatory bowel disease (IBD) patients in terms of pathogenesis, clinical manifestation, response to treatment, and prognosis, which requires the individualized and precision management of patients. Many studies have focused on prediction biomarkers and models for assessing IBD disease type, activity, severity, and prognosis. During the era of biologics, how to predict the response and side effects of patients to different treatments and how to quickly recognize the loss of response have also become important topics. Multiomics is a promising area for investigating the complex network of IBD pathogenesis. Integrating numerous amounts of data requires the use of artificial intelligence.


Assuntos
Inteligência Artificial , Doenças Inflamatórias Intestinais , Humanos , Medicina de Precisão/efeitos adversos , Doenças Inflamatórias Intestinais/diagnóstico , Doenças Inflamatórias Intestinais/genética , Doenças Inflamatórias Intestinais/terapia , Biomarcadores , Tecnologia
3.
Bioresour Technol ; 372: 128613, 2023 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-36640820

RESUMO

In this work, three biological ion exchange systems and one biological activated carbon (BAC) system were established by employing magnetic ion exchange resin (MIEX), non-magnetic resin (NIEX), polystyrenic resin (DIEX) and granular activated carbon as the biocarrier for advanced treatment of wastewater. Dissolved organic carbon (DOC) removal of four systems all stabilized at about 84% due to biodegradation. The start-up period of bio-MIEX (nearly 40 d) was greatly shorter than that of others (nearly 190 d). Ibuprofen removal was ascribed to adsorption in the initial stage, which subsequently changed to the effect of biodegradation. After the start-up period, ibuprofen removal was nearly 100% (bio-MIEX), 60% (bio-NIEX), 61% (bio-DIEX) and 89% (BAC). According to the surface observation, ATP and protein measurement and metagenomic analysis, the superior performance of bio-MIEX could be attributed to its highest biological activity resulted from the presence of Fe3O4 rather than polymer matrix and surface roughness.

4.
Molecules ; 28(2)2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36677934

RESUMO

Xanthomonas spp. are important plant pathogens that seriously endanger crop yields and food security. RpfF is a key enzyme that is involved in the synthesis of diffusible signal factor (DSF) signals and predominates in the signaling pathway regulating quorum sensing (QS) in Xanthomonas. Currently, novel RpfF enzyme-based quorum sensing agents have been proposed as a promising strategy for the development of new pesticides. However, few reports are available that comprehensively summarize the progress in this field. Therefore, we provide a comprehensive review of the recent advances in DSF-mediated QS and recently reported inhibitors that are proposed as bactericide candidates to target the RpfF enzyme and control plant bacterial diseases.


Assuntos
Xanthomonas , Percepção de Quorum , Proteínas de Bactérias/metabolismo , Transdução de Sinais
5.
J Clin Med ; 12(2)2023 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-36675468

RESUMO

Double-lumen endobronchial tube (DLT) intubation is more challenging than single-lumen tube intubation is, and the rigid video stylet (RVS) is one of the tools that has emerged to deal with this demanding intubation procedure. We evaluated whether the UE® RVS can shorten the DLT intubation time and improve the first-attempt intubation success rate compared with that of Macintosh laryngoscope (ML). A total of 130 participants scheduled to undergo thoracoscopic pulmonary surgeries were enrolled. They were randomized to receive either ML- or RVS-assisted DLT intubation. The primary outcomes were the intubation time and first-attempt intubation success rate. The secondary outcomes were the overall intubation success rate, mean arterial pressure, postoperative sore throat (POST), and postoperative hoarseness at 1 h and 24 h. Compared with the ML group, the intubation time was significantly shorter in the RVS group (p < 0.001; 30.82 ± 10.61 vs. 39.62 ± 6.54 s), however, the first-attempt success rate was significantly lower (p = 0.048; 83.08% vs. 95.16%). The POST at 1 h was less severe in the RVS group (p = 0.021). No significant differences were found for the other indicators. Among the patients with normal airways, the UE® RVS can achieve faster DLT intubation and decrease the severity of a POST at 1 h, although it was associated with a lower first-attempt intubation success rate.

6.
Proc Biol Sci ; 290(1990): 20221658, 2023 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-36629113

RESUMO

Human-induced biodiversity loss negatively affects ecosystem function, but the interactive effects of biodiversity change across trophic levels remain insufficiently understood. We sampled arboreal spiders and lepidopteran larvae across seasons in 2 years in a subtropical tree diversity experiment, and then disentangled the links between tree diversity and arthropod predator diversity by deconstructing the pathways among multiple components of diversity (taxonomic, phylogenetic and functional) with structural equation models. We found that herbivores were major mediators of plant species richness effects on abundance, species richness, functional and phylogenetic diversity of predators, while phylogenetic, functional and structural diversity of trees were also important mediators of this process. However, the strength and direction differed between functional, structural and phylogenetic diversity effects, indicating different underlying mechanisms for predator community assembly. Abundance and multiple diversity components of predators were consistently affected by tree functional diversity, indicating that the variation in structure and environment caused by plant functional composition might play key roles in predator community assembly. Our study highlights the importance of an integrated approach based on multiple biodiversity components in understanding the consequences of biodiversity loss in multitrophic communities.


Assuntos
Artrópodes , Aranhas , Animais , Humanos , Ecossistema , Filogenia , Biodiversidade , Plantas
8.
Thorac Cancer ; 2023 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-36630992

RESUMO

INTRODUCTION: This study investigated whether programmed death-ligand 1 (PD-L1) expression of circulating tumor cells (CTCs) in peripheral blood can serve as a predictive biomarker for immunotherapy efficacy in patients with advanced non-small-cell lung cancer (NSCLC). METHODS: We employed a negative enrichment method to isolate CTCs. We identified PD-L1 + CTCs as PD-L1+/4',6-diamidino-2-phenylindole (DAPI)+/CD45-circulating tumor cells through an immunofluorescence method. Tumor tissue PD-L1 expression was determined by immunohistochemical staining. The correlation between CTC PD-L1 expression and patients' prognostic features was estimated through the Kaplan-Meier method. RESULTS: CTCs released a higher detection rate of PD-L1 expression than tumor tissues (53.0% vs. 42.1%). No correlation was observed between them. Forty-nine NSCLC patients received anti-PD-1/PD-L1 immunotherapy (three with combined anti-PD-1/PD-L1 and cytotoxic T lymphocyte-associated antigen-4 (CTLA-4), two with four cycles of combined immune checkpoint inhibitors [ICIs] plus chemotherapy and ICI monotherapy for maintenance). Patients with PD-L1 expression on tissue or CTCs had a median progression-free survival (mPFS) of 5.6 months (n = 36, 95% confidence interval [CI] 3.6-7.5 months), significantly longer than those without PD-L1 detection (n = 9, mPFS of 1.4 months, 95% CI 1.3-1.5 months, log-rank p = 0.032). The multivariable Cox proportional-hazard model suggested that the tissue or CTC PD-L1 expression was associated with a lower risk of progression (hazard ratio 0.45, 95% CI 0.21-0.98, p = 0.043). CONCLUSIONS: CTCs and tumor tissues reveal heterogeneous expression of PD-L1 in NSCLC patients. Patients with baseline PD-L1 expression on CTCs or tissue showed prolonged mPFS and may help to identify the subsets of patients who potentially benefit from immunotherapy.

9.
Cancers (Basel) ; 15(1)2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36612298

RESUMO

MET inhibitors have shown promising efficacy for MET-dysregulated non-small cell lung cancer (NSCLC). However, quite a few patients cannot benefit from it due to the lack of powerful biomarkers. This study aims to explore the potential role of plasma proteomics-derived biomarkers for patients treated with MET inhibitors using mass spectrometry. We analyzed the plasma proteomics from patients with MET dysregulation (including MET amplification and MET overexpression) treated with MET inhibitors. Thirty-three MET-dysregulated NSCLC patients with longitudinal 89 plasma samples were included. We classified patients into the PD group and non-PD group based on clinical response. The baseline proteomic profiles of patients in the PD group were distinct from those in the non-PD group. Through protein screening, we found that a four-protein signature (MYH9, GNB1, ALOX12B, HSD17B4) could predict the efficacy of patients treated with MET inhibitors, with an area under the curve (AUC) of 0.93, better than conventional fluorescence in situ hybridization (FISH) or immunohistochemistry (IHC) tests. In addition, combining the four-protein signature with FISH or IHC test could also reach higher predictive performance. Further, the combined signature could predict progression-free survival for MET-dysregulated NSCLC (p < 0.001). We also validated the performance of the four-protein signature in another cohort of plasma using an enzyme-linked immunosorbent assay. In conclusion, the four plasma protein signature (MYH9, GNB1, ALOX12B, and HSD17B4 proteins) might play a substitutable or complementary role to conventional MET FISH or IHC tests. This exploration will help select patients who may benefit from MET inhibitors.

10.
Can J Infect Dis Med Microbiol ; 2023: 8079091, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36688009

RESUMO

The antibiotic resistance of the food-borne pathogen Vibrio parahaemolyticus has attracted researchers' attention in recent years, but its molecular mechanism remains poorly understood. In this study, 7 genes encoding outer membrane proteins (OMPs) were individually deleted in V. parahaemolyticus ATCC33846, and the resistance of these 7 mutants to 14 antibiotics was investigated. The results revealed that the resistance of the 7 mutants to ampicillin was significantly increased. Further exploration of 20-gene transcription changes by real time-qPCR (RT-qPCR) demonstrated that the higher ampicillin resistance might be attributed to the expression of ß-lactamase and reduced peptidoglycan (PG) synthesis activity through reduced transcription of penicillin-binding proteins (PBPs), increased transcription of l,d-transpeptidases, downregulated d,d-carboxypeptidase, and alanine deficiency. This study provides a new perspective on ampicillin resistance in OMP mutants with respect to PG synthesis.

11.
Eur J Radiol ; 160: 110689, 2023 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-36669332

RESUMO

OBJECTIVE: This study aimed to identify regions with at least 20% tumor cell content in lung cancer tumors by using spectral parameters from dual-layer spectral detector computed tomography (SDCT) to design the puncture path for transthoracic lung biopsy (TTLB). MATERIALS AND METHODS: This prospective study recruited patients with suspected lung cancer. Forty-one patients were enrolled to identify the high tumor cell proportion region (HTPR) and then another 15 patients to validate the accuracy of the HTPR. In each of the 41 patients, the suspected regions with high or low tumor cell proportions were punctured according to local iodine density (IoD) values for separate biopsies. The tumor cell proportions of 82 specimens were assessed and classified into high and low tumor cell proportions based on the threshold value of 20 %. The performance of spectral parameters was analyzed to distinguish the HTPR (tumor cell proportion ≥ 20 %) from the low tumor cell proportion region (LTPR). The cutoff value of optimal spectral parameter was used to prospectively guide the biopsy of the HTPR in 15 cases for further validation, and then the accuracy was calculated. RESULTS: The AUC values of spectral parameters were all higher than those of CTconventional in identifying the HTPR (all P < 0.05). The IoD with a cutoff value of 0.59 mg/mL in arterial phase (AP) yielded good performance (specificity: 97.10 %) in identifying the HTPR. It was applied to 15 cases for validation, and the accuracy rate was 100 %. CONCLUSION: Spectral CT parameters can be used to identify regions with at least 20% tumor cell content in lung cancer for biopsies.

12.
Cell Rep Med ; : 100911, 2023 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-36657446

RESUMO

Predicting the clinical response to chemotherapeutic or targeted treatment in patients with locally advanced or metastatic lung cancer requires an accurate and affordable tool. Tumor organoids are a potential approach in precision medicine for predicting the clinical response to treatment. However, their clinical application in lung cancer has rarely been reported because of the difficulty in generating pure tumor organoids. In this study, we have generated 214 cancer organoids from 107 patients, of which 212 are lung cancer organoids (LCOs), primarily derived from malignant serous effusions. LCO-based drug sensitivity tests (LCO-DSTs) for chemotherapy and targeted therapy have been performed in a real-world study to predict the clinical response to the respective treatment. LCO-DSTs accurately predict the clinical response to treatment in this cohort of patients with advanced lung cancer. In conclusion, LCO-DST is a promising precision medicine tool in treating of advanced lung cancer.

13.
Cell Signal ; : 110606, 2023 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-36681290

RESUMO

Metabolic reprogramming of macrophages initiates the polarization of pro-inflammatory macrophages that exacerbates adipocyte dysfunction and obesity. The imbalance of mitochondrial Ca2+ homeostasis impairs mitochondrial function and promotes inflammation. Connexin 43 (Cx43), a ubiquitous gap junction protein, has been demonstrated to regulate intracellular Ca2+ homeostasis. Here we explored whether macrophage Cx43 affects the obesity process by regulating the polarization of macrophage. HFD treatment induced obesity and exacerbated macrophages infiltration with upregulation of macrophages Cx43. Macrophage-specific knockout of Cx43 reduced HFD-induced obesity by alleviating inflammation in adipose tissue, with less pro-inflammatory M1 macrophage infiltration. Consistently, inhibition or knockdown of Cx43 improved palmitic acid (PA) induced mitochondrial dysfunction, as indicated by improved oxidative phosphorylation (OXPHOS), reduced formation of mitochondria-associated membranes (MAM) and mitochondrial Ca2+ overload. Mechanistically, Cx43 interacted with the mitochondrial Ca2+ uniporter (MCU) and knockdown of Cx43 alleviated PA-induced succinate dehydrogenase (SDH) oxidation by lowering MCU-mediated mitochondrial Ca2+ uptake, which then, promoting the polarization of pro-inflammatory M1 macrophages. Thus, this study identified Cx43 as a mitochondrial Ca2+ regulator that aggravates obesity via promoting macrophages polarized to M1 pro-inflammatory phenotype and suggests that Cx43 might be a promising therapeutic target antagonizing obesity.

14.
J Exp Child Psychol ; 227: 105601, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36512921

RESUMO

Drawing from two waves (∼1.5-2.5 years apart) of longitudinal data, the current study investigated the bidirectional associations between self-regulatory skills and mathematics achievement among a socioeconomically diverse sample of school-age Chinese American children from immigrant families (N = 258; 48.1% girls; ages 5.8-9.1 years; first to third grades at Wave 1). Children's self-regulatory skills were assessed with task-based measures of attention focusing, inhibitory control, behavioral persistence, and comprehensive executive function as well as parent- and teacher-reported effortful control. Multiple regressions showed that behavioral persistence and parent-reported effortful control positively predicted math achievement over time. Math achievement positively predicted comprehensive executive function over time. These effects were found when controlling for child age, sex, generation status, family socioeconomic status, parents' cultural orientations, and prior levels of math achievement or self-regulation. The prospective relation of math achievement predicting comprehensive executive function remained significant after a false discovery rate correction.


Assuntos
Emigrantes e Imigrantes , Feminino , Humanos , Criança , Pré-Escolar , Masculino , Instituições Acadêmicas , Pais , Matemática , Estudos Longitudinais
15.
Exp Eye Res ; 226: 109347, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36502924

RESUMO

Retinopathy of prematurity (ROP) is a vision-threatening ocular disease that occurs in premature infants, but the underlying mechanism is still unclear. Since oxidative stress has been well documented in the ROP development, we aimed to investigate whether ferroptosis, a new type of cell death characterized by lipid peroxidation and iron overload, is also involved in ROP. We detected the lipid peroxidation, oxidative stress and the expression of ferroptosis markers in the retina of mouse model of oxygen-induced retinopathy. After ferroptosis inhibitor, ferrostatin-1, was administered by intravitreal injection, ferroptosis marker, lipid peroxidation, retinal vasculature and glial cell activation were examined. We found decreased expression of SLC7A11 and GPX4, increased expression of FTH1 and TFRC, as well as increase of lipid peroxidation in the retina of OIR mice. Ferrostatin-1 administration significantly reduced lipid peroxidation, and also reversed the change of ferroptosis marker. Neovascular area and avascular area were suppressed and the pathological vasculature changes including acellular vessels and ghost pericytes were decreased. Microglial cell and Müller cell activation was not evidently influenced by ferrostatin-1 treatment. Our findings suggest that ferroptosis is involved in the pathological angiogenesis and might be a promising target for ROP therapy.


Assuntos
Ferroptose , Retinopatia da Prematuridade , Humanos , Recém-Nascido , Animais , Camundongos , Oxigênio/toxicidade , Ferroptose/fisiologia , Neovascularização Patológica , Retinopatia da Prematuridade/metabolismo
16.
ACS Biomater Sci Eng ; 9(1): 437-448, 2023 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-36508691

RESUMO

Local causes of slow wound healing include infection and wound hemorrhage. Using sodium bicarbonate as a neutralizer, a variety of carboxymethyl chitosan-tannic acid (CMC-TA) composite hydrogels solidify through hydrogen bonding in this study. The best-performing hydrogel was synthesized by altering the concentration of TA and exhibited remarkable mechanical properties and biocompatibility. Following in vitro characterization tests, the CMC-TA hydrogel exhibited remarkable antibacterial and antioxidant properties, as well as quick hemostasis capabilities. In the in vivo wound healing study, the results showed that the CMC-TA hydrogel could relieve inflammation and promote the recovery of skin incision, re-epithelialization, and collagen deposition. Overall, this multifunctional hydrogel could be an ideal wound dressing for the clinical therapy of full-thickness wounds.


Assuntos
Quitosana , Quitosana/farmacologia , Hidrogéis/farmacologia , Antioxidantes/farmacologia , Cicatrização , Antibacterianos/farmacologia , Hemostasia
17.
Water Res ; 229: 119458, 2023 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-36516492

RESUMO

Halophenolic disinfection byproducts (DBPs) are a class of emerging pollutants whose adverse effects on human cells and the underlying molecular mechanisms still need further exploration. In this study, we found that when halophenolic DBPs were substituted with the same halogen, the more substitution sites, the more cytotoxic, while when they were substituted at the same sites, the most toxic chemical was iodophenols, followed by bromophenols and chlorophenols. In addition, several of them exerted significant endocrine-disrupting effects at sublethal concentrations. 2,4,6-triiodophenol (TIP) and 2,4-dichlorophenol (2,4-DCP) showed the highest estradiol equivalent factor (EEF) of 4.41 × 10-8 and flutamide equivalent factor (FEF) of 0.4, respectively. Furthermore, all of the halophenolic DBPs except for 2-chlorophenol (2-CP) and 2-bromophenol (2-BP) significantly increased the levels of reactive oxygen species (ROS) or 8-hydroxydeoxyguanosine (8-OHdG) in HepG2 cells. The lowest cytotoxicity and unchanged ROS and 8-OHdG levels after 2-CP exposure may result from the activation of the transporters of the adenosine triphosphate (ATP) binding cassette in cells. Transcriptome analysis revealed distinct grouping patterns of 2-CP, 2,6-dibromophenol (2,6-DBP), and TIP at the concentrations of EC20, and the top differentially expressed genes (DEGs) were involved in the antioxidant-, immune-, and endocrine-associated systems. The weighted gene correlation network analysis well connected the phenotypes (EC50, EEF, FEF, ROS, 8-OHdG, and ABC transporters) with the DEGs and revealed that the MAPK signaling pathway played a vital role in regulating the biological response after exposure to halophenolic DBPs. This study provides deep insights into the underlying mechanisms of the toxic effects induced by halophenolic DBPs.


Assuntos
Desinfetantes , Poluentes Químicos da Água , Purificação da Água , Humanos , Desinfecção , Desinfetantes/toxicidade , Desinfetantes/química , Espécies Reativas de Oxigênio , Halogenação , Poluentes Químicos da Água/química , Estresse Oxidativo , Perfilação da Expressão Gênica
18.
J Adv Res ; 43: 205-218, 2023 01.
Artigo em Inglês | MEDLINE | ID: mdl-36585109

RESUMO

Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease characterized by oxidative stress that triggers motor neurons loss in the brain and spinal cord. However, the mechanisms underlying the exact role of oxidative stress in ALS-associated neural degeneration are not definitively established. Oxidative stress-generated phospholipid peroxides are known to have extensive physiological and pathological consequences to tissues. Here, we discovered that the deficiency of glutathione peroxidase 4 (GPX4), an essential antioxidant peroxidase, led to the accumulation of phospholipid peroxides and resulted in a loss of motor neurons in spinal cords of ALS mice. Mutant human SOD1G93A transgenic mice were intrathecally injected with neuron-targeted adeno-associated virus (AAV) expressing GPX4 (GPX4-AAV) or phospholipid peroxidation inhibitor, ferrostatin-1. The results showed that impaired motor performance and neural loss induced by SOD1G93A toxicity in the lumbar spine were substantially alleviated by ferrostatin-1 treatment and AAV-mediated GPX4 delivery. In addition, the denervation of neuron-muscle junction and spinal atrophy in ALS mice were rescued by neural GPX4 overexpression, suggesting that GPX4 is essential for the motor neural maintenance and function. In comparison, conditional knockdown of Gpx4 in the spinal cords of Gpx4fl/fl mice triggered an obvious increase of phospholipid peroxides and the occurrence of ALS-like motor phenotype. Altogether, our findings underscore the importance of GPX4 in maintaining phospholipid redox homeostasis in the spinal cord and presents GPX4 as an attractive therapeutic target for ALS treatment.


Assuntos
Esclerose Amiotrófica Lateral , Doenças Neurodegenerativas , Camundongos , Humanos , Animais , Esclerose Amiotrófica Lateral/genética , Esclerose Amiotrófica Lateral/patologia , Superóxido Dismutase-1/genética , Doenças Neurodegenerativas/patologia , Superóxido Dismutase/genética , Neurônios Motores/patologia , Camundongos Transgênicos , Glutationa Peroxidase/genética , Peróxidos
19.
Environ Sci Technol ; 57(1): 509-519, 2023 01 10.
Artigo em Inglês | MEDLINE | ID: mdl-36538014

RESUMO

Despite the high removal efficiency for chemical pollutants by tertiary wastewater treatment processes (TWTPs), there is no definite conclusion in terms of microbial risk mitigation yet. This study utilized metagenomic approaches to reveal the alterations of antibiotic resistance genes (ARGs), virulence factor genes (VFGs), their co-occurrence, and potential hosts during multiple TWTPs. Results showed that the TWTPs reduced chemical pollutants in wastewater, but the denitrifying biofilter (DB) significantly increased the absolute abundances of selected antibiotic-resistant bacteria and ARGs, and simultaneously elevated the relative abundances of ARGs and VFGs through the enrichment of multidrug resistance and offensive genes, respectively. Moreover, the co-occurrence of ARGs and VFGs (e.g., bacA-tapW, mexF-adeG) was only identified after the DB treatment and all carried by Pseudomonas. Then, the ultraviolet and constructed wetland treatment showed good complementarity for microbial risk reduction through mitigating antibiotic resistance and pathogenicity. Network and binning analyses showed that the shift of key operational taxonomic units affiliating to Pseudomonas and Acinetobacter may contribute to the dynamic changes of ARGs and VFGs during the TWTPs. Overall, this study sheds new light on how the TWTPs affect the antibiotic resistome and VFG profiles and what TWTPs should be selected for microbial risk mitigation.


Assuntos
Genes Bacterianos , Purificação da Água , Antibacterianos/farmacologia , Farmacorresistência Bacteriana/genética , Virulência , Melhoria de Qualidade
20.
J Colloid Interface Sci ; 634: 897-905, 2023 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-36566635

RESUMO

Aiming at the sluggish water dissociation step in alkaline hydrogen evolution reaction (HER), the platinum-nickel alloy material (PtNi10/C) featuring unique crystalline/amorphous structure supported on carbon black is deliberately designed and fabricated via a reversely rapid co-precipitation and mild thermal reduction strategy. Electrochemical results show that only 66 mV of overpotential is needed for PtNi10/C to drive a current density of 10 mA cm-2 at a lower platinum loading (8.3 µgPt cm-2 geo), which is much lower than that of other catalysts with a single metal source(S-Ni/C and S-Pt/C) and even the commercial Pt/C catalyst (20 wt%). The target catalyst also exhibits smaller tafel slope value (16.73 mV dec-1) and electrochemical impedance value, enabling a fast kinetics rate for water dissociation. Partial crystallization facilitates moderate adsorption of intermediates, while the high-valence Ni(II) and Pt(II) species serve as pivotal driving force for the kinetic dissociation of water. The unique microstructure of PtNi10/C shows a remarkable advantage toward HER in alkaline but acidic medium. In addition, other transition metal-based catalysts following the similar protocol are also fabricated and present varying degrees of HER performance. Hence, the facile and rapid co-precipitation/thermal reduction strategy proposed in this study provides some guidelines for designing high-efficiency alkaline HER catalysts.

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