Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 258
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Inorg Chem ; 2020 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-32045227

RESUMO

Colorimetric assays have drawn increasing research interest with respect to the quantitative detection of hydrogen peroxide (H2O2) based on artificial enzymes because of their advantages with respect to natural enzymes, including design flexibility, low cost, and high stability. Regardless, the majority of the artificial enzymes exhibit low affinity to H2O2 with large Michaelis-Menten constants (Km). This indicates that the catalytic oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to blue-colored oxTMB requires a high H2O2 concentration, hindering the sensitivity of the colorimetric assay. To address this problem, novel reduced Co3O4 nanoparticles (R-Co3O4) have been synthesized in this study via a step-by-step procedure using ZIF-67 as the precursor. R-Co3O4 exhibits a considerably enhanced peroxidase-like activity when compared with that exhibited by pristine Co3O4 (P-Co3O4). The catalytic process in the case of R-Co3O4 occurs in accordance with the typical Michaelis-Menten equation, and the affinity of R-Co3O4 to H2O2 is apparently higher than that of P-Co3O4. Furthermore, the density functional theory calculations revealed that the introduction of oxygen vacancies to R-Co3O4 enhances its H2O2 adsorption ability and facilitates the decomposition of H2O2 to produce ·OH radicals, resulting in improved peroxidase-like activity. A simple and convenient colorimetric assay has been established based on the excellent peroxidase-like activity of R-Co3O4 for detecting H2O2 in concentrations of 1-30 µM with a detection limit of 4.3 × 10-7 mol/L (S/N = 3). Furthermore, the R-Co3O4-based colorimetric method was successfully applied to glucose detection in human serum samples, demonstrating its potential for application in complex biological systems.

2.
Mol Pain ; : 1744806920909993, 2020 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-32052691

RESUMO

BACKGROUND: Bone cancer pain (BCP) is common in patients with advanced cancers as tumor metastasizes to bone. The inefficient clinical treatment severely reduces quality of life of BCP patients. During the pain status, activated spinal astrocytes and microglia release various inflammatory cytokines, resulting in spinal inflammation and the development of neuron sensitization. Scorpion is the dry body of Buthus martensii Karsch (BMK), and is often used for various pain management in clinical practice. However, its function on BCP is unclear. METHODS: We investigated the effects of intragastric administration of scorpion on bone cancer pain induced by left tibial cavity injection of Walker 256 cells. Nociceptive behavior was measured using the von Frey filaments test and the spontaneous ambulatory pain score (SAPS). The bone destruction was assessed by tibial radiographs. Expression of spinal cord astrocyte marker GFAP and microglial marker Iba1 was monitored by western blot assay and immunofluorescence. TNF-α, IL-6 and IL-1ß was detected by real-time PCR. The proliferation of Walker 256 cells was evaluated by CCK8 assay. RESULTS: Intragastric administration of scorpion reduced bone cancer pain behavior and relieved bone destruction, accompanied by decreased expression of spinal GFAP and Iba1 protein level and TNF-α, IL-6 and IL-1ß mRNA level. Besides, scorpion inhibited proliferation of Walker 256 cells in a dose- and time-dependent manner. CONCLUSION: Our results demonstrate that scorpion produces an analgesic effect in a rat model of bone cancer pain via inhibiting bone destruction and activation of spinal cord astrocytes and microglia.

3.
Artigo em Inglês | MEDLINE | ID: mdl-32040033

RESUMO

Resveratrol (Res) was recently reported to ameliorate hypoxia/reoxygenation (H/R)-caused oxidative stress in H9c2 cardiomyocytes through promoting the mitochondrial translocation of DJ-1 protein and subsequently preserving the activity of mitochondrial complex I. However, it is noteworthy that DJ-1 possesses no mitochondria-targeting sequence. Therefore, how Res induces DJ-1 mitochondrial translocation is an important and interesting question for further exploration. Glucose regulated protein 75 (Grp75), whose N-terminus contains a 51-amino acid long mitochondrial-targeting signal peptide, is a cytoprotective chaperone that partakes in mitochondrial import of several proteins. Here, the contribution of Grp75 to mitochondrial import of DJ-1 by Res was investigated in a cellular model of H/R. Our results showed that Res upregulated the expression of DJ-1 protein, enhanced the interaction of DJ-1 and Grp75, and promoted DJ-1 translocation to mitochondria from cytosol in H9c2 cardiomyocytes undergoing H/R. Importantly, knockdown of Grp75 markedly reduced the interaction of DJ-1 with Grp75 and subsequent DJ-1 mitochondrial translocation induced by Res. Furthermore, Res pretreatment promoted the association of DJ-1 with ND1 and NDUFA4 subunits of complex I, preserved the activity of complex I, decreased mitochondria-derived reactive oxygen species production, and eventually ameliorated H/R-caused oxidative stress damage. Intriguingly, these effects were largely prevented also by siRNA targeting Grp75. Overall, these results suggested that Grp75 interacts with DJ-1 to facilitate its translocation from cytosol to mitochondria, which is required for Res-mediated preservation of mitochondria complex I and cardioprotection from H/R-caused oxidative stress injury.

4.
Artigo em Inglês | MEDLINE | ID: mdl-31939867

RESUMO

BACKGROUND: Hirschsprung's disease (HSCR) is the most common congenital gut motility disorder, involving a severe anomaly of the enteric nervous system (ENS), and is characterized by functional intestinal obstruction due to lack of intrinsic innervation (aganglionosis) in the distal bowel. OBJECTIVE: The aim of this study was to examine the distribution patterns of collagens I (Col I), III (Col III) and IV (Col IV) in the ENS of HSCR patients, in order to determine whether or not collagen levels are altered in the aganglionic bowel. METHODS: We measured the expression levels of Col I, Col III and Col IV in colonic muscle from 129 children with HSCR. The localizations of the three collagens and myenteric ganglia were assessed morphologically by immunofluorescence staining. Western blots (WB) and real-time fluorescence quantitative PCR (qRT-PCR) were performed to examine the relative levels of these collagens in aganglionic, transitional, and ganglionic colon segments. RESULTS: Immunoreactivities of Col I and Col III were high around and within myenteric ganglia in the ganglionic segment, moderate in the transitional segment, and weak in the aganglionic segment. Col IV immunoreactivity showed the opposite pattern, being lowest in the ganglionic segment and highest in the aganglionic segment. CONCLUSION: Col I and Col III are decreased and Col IV is increased in the distal colon of HSCR patients.

5.
Ecotoxicol Environ Saf ; 188: 109895, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-31706238

RESUMO

Seventeen soil samples collected in an industrial park located in Ningxia Province, Northwestern China were analyzed for polychlorinated naphthalenes (PCNs), polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), and polychlorinated biphenyls (PCBs). The PCN, PCDD/F, and PCB concentration ranges were 183-3340, 7.00-215, and 45.1-355 pg/g, respectively. Positive matrix factorization showed that secondary ferrous metal smelters and cement kilns contributed more than 70% of the total PCN concentration. Historical use of Halowax 1051 also affected the PCN concentrations in soil. Principal component analysis indicated that the PCDD/F concentrations in soil in the study area were mainly affected by thermal processes in secondary ferrous metal smelters. CB-209 was an important contributor to total PCBs in the study area, and likely originated from the phthalocyanine-type pigments used in a local recycled paper mill. Samples S10, S1, S17, and S6 had high ∑TEQ (PCDD/Fs + PCNs + PCBs) concentrations, and the carcinogenic risks of PCDD/Fs, PCNs, and PCBs for workers from these samples were 0.487 × 10-6, 0.234 × 10-6, 0.230 × 10-6, and 0.210 × 10-6, respectively. According to our results, the health risks of PCDD/Fs, PCNs, and PCBs for workers in this area should be given more attention.

6.
J Am Chem Soc ; 142(3): 1614-1620, 2020 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-31887253

RESUMO

Understanding the pathological process of biological systems can greatly improve the prevention and treatment of diseases. The study of pathological processes has now reached the molecular level, and molecular fluorescent probes have become a powerful tool. Chromene, also known as benzo-pyran molecule, is a structural element of natural products with good biological compatibility and was developed as a fluorescent probe. The thiol-chromene "click" nucleophilic pyran ring-opening reaction allows the quick detection of thiol. In this work, the chromene alcohol can function as an efficient self-immolative spacer, which covalently links NIR fluorophore via a carbonyl ester. Due to its favorable characteristics and superior applicability, the self-immolative amplifier NIR-HMPC achieves the specific, rapid, sensitive, NIR fluorescent detection of thiols. Furthermore, the indoles iodized salt in the system can specifically target thiols in mitochondria. Thus, this probe was used to visualize the fluctuations of thiols during oxidative stress and cell apoptosis, cerebral ischemia reperfusion, demonstrating that it is valuable for elucidating pathophysiology process in living organism. This discovery provides an effective means for studying the pathological process of thiol related diseases.

7.
PLoS One ; 14(12): e0226100, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31805153

RESUMO

Sophora alopecuroides (Faboideae) is an endemic species, mainly distributed in northwest China. However, the limited molecular markers range for this species hinders breeding and genetic studies. A total of 20,324 simple sequence repeat (SSR) markers were identified from 118,197 assembled transcripts and 18 highly polymorphic SSR markers were used to explore the genetic diversity and population structure of S. alopecuroides from 23 different geographical populations. A relatively low genetic diversity was found in S. alopecuroides based on mean values of the number of effective alleles (Ne = 1.81), expected heterozygosity (He = 0.39) and observed heterozygosity (Ho = 0.55). The results of AMOVA indicated higher levels of variation within populations than between populations. Bayesian-based cluster analysis, principal coordinates analysis and Neighbor-Joining phylogeny analysis roughly divided all genotypes into four major groups with some admixtures. Meanwhile, geographic barriers would have restricted gene flow between the northern and southern regions (separated by Tianshan Mountains), wherein the two relatively ancestral and independent clusters of S. alopecuroides occur. History trade and migration along the Silk Road would together have promoted the spread of S. alopecuroides from the western to the eastern regions of the northwest plateau in China, resulting in the current genetic diversity and population structure. The transcriptomic SSR markers provide a valuable resource for understanding the genetic diversity and population structure of S. alopecuroides, and will assist effective conservation management.

8.
Molecules ; 25(1)2019 Dec 24.
Artigo em Inglês | MEDLINE | ID: mdl-31878239

RESUMO

DJ-1 was recently reported to be involved in the cardioprotection of hypoxic preconditioning (HPC) against hypoxia/reoxygenation (H/R)-induced oxidative stress damage, by preserving mitochondrial complex I activity and, subsequently, inhibiting mitochondrial reactive oxygen species (ROS) generation. However, the molecular mechanism by which HPC enables mitochondrial translocation of DJ-1, which has no mitochondria-targeting sequence, to preserve mitochondrial complex I, is largely unknown. In this study, co-immunoprecipitation data showed that DJ-1 was associated with glucose-regulated protein 75 (Grp75), and this association was significantly enhanced after HPC. Immunofluorescence imaging and Western blot analysis showed that HPC substantially enhanced the translocation of DJ-1 from cytosol to mitochondria in H9c2 cells subjected to H/R, which was mimicked by DJ-1 overexpression induced by pFlag-DJ-1 transfection. Importantly, knockdown of Grp75 markedly reduced the mitochondrial translocation of DJ-1 induced by HPC and pFlag-DJ-1 transfection. Moreover, HPC promoted the association of DJ-1 with mitochondrial complex I subunits ND1 and NDUFA4, improved complex I activity, and inhibited mitochondria-derived ROS production and subsequent oxidative stress damage after H/R, which was also mimicked by pFlag-DJ-1 transfection. Intriguingly, these effects of HPC and pFlag-DJ-1 transfection were also prevented by Grp75 knockdown. In conclusion, these results indicated that HPC promotes the translocation of DJ-1 from cytosol to mitochondria in a Grp75-dependent manner and Grp75 is required for DJ-1-mediated protection of HPC on H/R-induced mitochondrial complex I defect and subsequent oxidative stress damage.

9.
Artigo em Inglês | MEDLINE | ID: mdl-31759882

RESUMO

Hydrazine is extremely harmful to the human body. The leakage of hydrazine is liable to cause potential safety hazards. Here, we reported a new fluorescence probe based on the tandem reaction. The hydrazine-triggered hydrazinolysis-cyclization resulted in the formation of the iminocoumarin. The fluorescence intensity at 522 nm of the probe increased after the reaction with hydrazine. There was a linear relationship between the fluorescence intensity and the concentration of hydrazine (0.14-120.00 µM). The LOD of the probe to N2H4 was 1.36 ppb. Notably, the probe could detect hydrazine in BT474 cells and tap water.

10.
Cell Mol Neurobiol ; 2019 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-31760535

RESUMO

Although approximately 50% of cases have a known genetic defect, the precise pathogenesis of Hirschsprung disease (HSCR) is still unclear. We recently reported that expression of fibronectin (FN), which is involved in the migration, colonization, and differentiation of enteric neural crest cells (ENCCs), is increased in aganglionic colonic segments obtained from patients. We hypothesized that abnormally high levels of FN might play a role in the etiology of HSCR. Here, to test this hypothesis, we investigated aganglionic, transitional, and ganglionic colon segments from 63 children with HSCR and distal colon from thirty healthy Wistar rats at embryonic day 20, in addition to in vitro studies with PC12 Adh neural crest cells. We measured the protein and mRNA expression levels of FN, together with a panel of excitatory (VGLUT1, GluA1, GluN1, PSD-95, and NL-1) and inhibitory (GAD67, GABA AR-α1, NL-2, and SLC32) synaptic markers. Expression of all these synaptic markers was significantly decreased in aganglionic colon, compared to ganglionic colon, whereas expression of FN was significantly increased. In a neural crest cell line, PC12 Adh, knockdown of FN with small-interfering RNA increased the expression of synaptic markers. Co-culture of colons from embryonic day 20 rats with RGD recombinant protein, which contains the RGD motif of FN, reduced the expression of excitatory and inhibitory synaptic markers. These results are consistent with the idea that the etiology of HSCR involves aberrant overexpression of FN, which may impair synaptic function and enteric nervous system development, leading to motor dysfunction of intestinal muscles.

11.
BMC Infect Dis ; 19(1): 940, 2019 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-31699037

RESUMO

BACKGROUND: Bacillus anthracis causes a highly lethal infectious disease primarily due to toxin-mediated injury. Antibiotics are no longer effective to treat the accumulation of anthrax toxin, thereby new strategies of antibody treatment are essential. Two anti- anthrax protective antigen (PA) antibodies, hmPA6 and PA21, have been reported by our lab previously. METHODS: The mechanisms of the two antibodies were elucidated by Electrophoresis, Competitive Enzyme-linked immune sorbent assay, Western blot analysis and immunoprecipitation test, and in vitro, in vivo (F344 rats) treatment test. The epitopes of the two antibodies were proved by Western blot and Enzyme-linked immune sorbent assay with different domains of PA. RESULTS: In this study, we compared affinity and neutralization of these two antibodies. PA21 was better in protecting cells and rats, whereas hmPA6 had higher affinity. Furthermore, the neutralization mechanisms of the two antibodies and their recognition domains of PA were studied. The results showed that hmPA6 recognized domain IV, thus PA could not bind to cell receptors. Conversely, PA21 recognized domain II, thereby limiting heptamer oligomerization of PA63 in cells. CONCLUSIONS: Our studies elucidated the mechanisms and epitopes of hmPA6 and PA21. The present investigation can advance future use of the two antibodies in anthrax treatment or prophylaxis, and potentially as a combination treatment as the antibodies target different epitopes.


Assuntos
Anticorpos Antibacterianos/metabolismo , Anticorpos Neutralizantes/metabolismo , Bacillus anthracis/imunologia , Animais , Antraz/imunologia , Anticorpos Antibacterianos/imunologia , Anticorpos Antibacterianos/farmacologia , Anticorpos Neutralizantes/imunologia , Anticorpos Neutralizantes/farmacologia , Antígenos de Bactérias/toxicidade , Toxinas Bacterianas/toxicidade , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Eletroforese , Epitopos/análise , Epitopos/imunologia , Imunoensaio , Ratos , Ratos Endogâmicos F344
12.
Exp Mol Med ; 51(11): 132, 2019 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-31704909

RESUMO

FoxM1 is involved in the regeneration of several organs after injury and expressed in the intestinal mucosa. The intrinsic mechanism of FoxM1 activity in the mucosa after intestinal ischemia/reperfusion (I/R) injury has not been reported. Therefore, we investigated the role of FoxM1 in mediating intestinal mucosa regeneration after I/R injury. Expression of FoxM1 and the proliferation of intestinal mucosa epithelial cells were examined in rats with intestinal I/R injury and an IEC-6 cell hypoxia/reperfusion (H/R) model. The effects of FoxM1 inhibition or activation on intestinal epithelial cell proliferation were measured. FoxM1 expression was consistent with the proliferation of intestinal epithelial cells in the intestinal mucosa after I/R injury. Inhibition of FoxM1 expression led to the downregulation of Ki-67 expression mediated by the inhibited expression of Nurr1, and FoxM1 overexpression promoted IEC-6 cell proliferation after H/R injury through activating Nurr1 expression. Furthermore, FoxM1 directly promoted the transcription of Nurr1 by directly binding the promoter of Nurr1. Further investigation showed low expression levels of FoxM1, Nurr1, and Ki-67 in the intestinal epithelium of patients with intestinal ischemic injury. FoxM1 acts as a critical regulator of intestinal regeneration after I/R injury by directly promoting the transcription of Nurr1. The FoxM1/Nurr1 signaling pathway represents a promising therapeutic target for intestinal I/R injury and related clinical diseases.

13.
Artigo em Inglês | MEDLINE | ID: mdl-31706823

RESUMO

BACKGROUND/PURPOSE: SraP is a serine-rich repeat protein (SRRP) from Staphylococcus aureus that binds to sialylated receptors to promote bacterial adhesion to and invasion into host epithelial cells, mediated by the l-lectin module of its ligand-binding region. METHODS: The sequence encoding the L-lectin module of SraP was inserted into pET28a plasmid, and the recombinant protein was purified by His label affinity chromatography. A monoclonal antibody (mAb) against the l-lectin module was obtained and confirmed by enzyme-linked immunosorbent assay and western blotting. The effect of the mAb on S. aureus adhesion and invasion was assessed in A549 cells and mice subjected to S. aureus challenge. RESULTS: We successfully obtained a mAb against the l-lectin module of SraP. Pre-incubation with the mAb dramatically inhibited the bacteria's ability to adhere to and invade A549 cells. Moreover, mice administered mAb through tail vein injection had significantly fewer bacteria in the blood. CONCLUSION: The anti-SraPL-Lectin mAb significantly reduced the adherence and invasion of S. aureus to host cells. This study lays the foundation for the future development of the l-lectin module of SraP as a target for the prevention and treatment of S. aureus infection. Our findings suggest that specific subdomains of SRRPs may represent potential antibacterial drug targets for intervention.

14.
Support Care Cancer ; 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31720804

RESUMO

PURPOSE: Both off-topic discussions and exchanges of social support are important to the success of online health support groups. Analyzing their relationship could enhance our understanding of the nature of helpful interactions in online cancer support groups and ways promoting their success. METHODS: A total of 15,284 messages were collected and analyzed from an online support group for rectal cancer. Two coders coded and categorized the messages into 211 threads using directed content analysis and a social support classification system. The relationship between off-topic discussions and social support was explored using the quadratic assignment procedure. RESULTS: There are 91 threads of off-topic discussions, 83 threads of informational support, 22 threads of emotional support, seven threads of tangible support, five threads of network support, and three threads of esteem support. More of the off-topic discussions are associated with more emotional and tangible support. Both off-topic discussions and informational support are mutually influenced by the mediating role of emotional support. In addition, off-topic discussions and network support are mutually influenced by the mediating role of emotional and tangible support, and off-topic discussions and esteem support are mutually influenced by the mediating role of tangible support. CONCLUSIONS: Off-topic discussions directly or indirectly promote different types of social support in an online rectal cancer support group.

15.
J Chem Phys ; 151(15): 154111, 2019 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-31640352

RESUMO

Molecular dynamics simulations require accurate force fields (FFs) to describe the physical and chemical properties of complex materials and systems. FF parameters for valence interactions can be determined from high-quality Quantum Mechanical (QM) calculations. However, it has been challenging to extract long-range nonbonded interaction potentials from QM calculations since there is no unambiguous method to separate the total QM energy into electrostatics (polarization), van der Waals (vdW), and other components. Here, we propose to use density functional theory with dispersion corrections to obtain the equation of state for single element solid systems (of H, C, N, O, F, Cl, Br, I, P, He, Ne, Ar, Kr, Xe, and Rn) from which we obtain the pure 2-body vdW nonbonded potentials. Recently, we developed the polarizable charge equilibration (PQEq) model based on QM polarization energy of electric probe dipoles with no contributions from vdW. Together, the vdW and PQEq interactions form the nonbonded potential of our new transferrable reactive FF (RexPoN). They may also be useful to replace the nonbonded parts of standard FFs, such as OPLS, Amber, UFF, and CHARMM. We find that the individual 2-body vdW potential curves can be scaled to a universal vdW potential using just three specific atomic parameters. This simplifies extension to the rest of the periodic table for atoms that do not exhibit molecular packing. We validate the accuracy of these nonbonded interactions for liquid water, energetic, and biological systems. In all cases, we find that our new nonbonded potentials provide good agreement with QM and experimental data.

16.
J Thorac Dis ; 11(9): 3721-3731, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31656644

RESUMO

Background: Esophageal cancer is a lethal disease of global scope. Radiotherapy is the main method to treat esophageal cancer; however, it concurrently leads to malnutrition. Intensity-modulated radiotherapy (IMRT) is superior to three-dimensional conformal radiation therapy (3D-CRT) in dosimetry and clinical outcomes. In this cohort study, we aimed to compare the effect of 3D-CRT and IMRT on malnutrition status. Methods: We retrospectively included 79 esophageal cancer patients (IMRT: n=27, 3D-CRT: n=52) who received radiotherapy. We collected nutrition indexes at the beginning, the second week, and the end of radiotherapy. Paired-T test was used to evaluate the nutrition status during radiotherapy in each group. Chi-square test and independent-sample T-test were applied to compare the dynamic changes of indexes between IMRT and 3D-CRT groups. Results: The baselines of the two groups are comparable. Nutrition Risk Screening 2002 (NRS-2002) score, body weight, BMI, hemoglobin, lymphocyte, total protein, and albumin values were significantly reduced during radiotherapy in both groups. The dynamic changes of nutrition indexes during radiotherapy were not significantly different between the IMRT and 3D-CRT groups. Besides, no difference was found for radiation esophagitis or treatment completion between the two groups. Conclusions: Malnutrition occurs in esophageal cancer patients during radiotherapy. IMRT did not significantly decrease the risk of malnutrition compared to 3D-CRT.

17.
Transl Lung Cancer Res ; 8(4): 367-379, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31555512

RESUMO

Background: Bone is one of the common metastatic sites of lung cancer, and its prognosis is not optimistic. We performed a study to evaluate the incidence, survival, and prognostic factors of lung cancer with bone metastasis (LCBM) at initial diagnosis, and to develop a nomogram to predict its outcomes. Methods: We conducted a retrospective study choosing 13,541 patients with LCBM from the Surveillance, Epidemiology, and End Results (SEER) 18 registry database. An X-tile analysis provided the optimal age cutoff point. The incidence, overall survival, and prognosis of bone metastasis were evaluated according to the patient information, characteristics of the tumor, and therapy. We also used multivariable Cox regression to estimate mortality hazard ratios (HRs) among patients with LCBM, while a visual nomogram was established to judge the prognosis. Results: The incidence of disease increased with age, but survival rates show the opposite trend. The median survival time was about 4 months. In addition, although the differences for patient race is not significant (P=0.445), White patients are prone to have bone metastases from lung cancer according to the incidence analysis. The difference for laterality is also not significant (P=0.534), while the factors of age, gender, the total number of sites, histological types, grade, tumor size, and treatment are significantly related to the outcome of patients with LCBM. Furthermore, our nomogram could predict the probability of surviving to the median survival time of the population with a c-index of 0.72. Conclusions: Age, characteristics of the tumor, and therapy should be considered for prediction of prognosis for patients with lung cancer bone metastasis. Putatively, the younger patients and the patients with chemotherapy and surgery may indicate improved survival.

18.
Front Microbiol ; 10: 2030, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31551967

RESUMO

Acquisition of the bla NDM- 1 gene by Proteus mirabilis is a concern because it already has intrinsic resistance to polymyxin E and tigecycline antibiotics. Here, we describe a P. mirabilis isolate that carries a pPrY2001-like plasmid (pHFK418-NDM) containing a bla NDM- 1 gene. The pPrY2001-like plasmid, pHFK418-NDM, was first reported in China. The pHFK418-NDM plasmid was sequenced using a hybrid approach based on Illumina and MinION platforms. The sequence of pHFK418-NDM was compared with those of the six other pPrY2001-like plasmids deposited in GenBank. We found that the multidrug-resistance encoding region of pHFK418-NDM contains ΔTn10 and a novel transposon Tn6625. Tn6625 consists of ΔTn1696, Tn6260, In251, ΔTn125 (carrying bla NDM- 1), ΔTn2670, and a novel mph(E)-harboring transposon Tn6624. In251 was first identified in a clinical isolate, suggesting that it has been transferred efficiently from environmental organisms to clinical isolates. Genomic comparisons of all these pPrY2001-like plasmids showed that their relatively conserved backbones could integrate the numerous and various accessory modules carrying multifarious antibiotic resistance genes. Our results provide a greater depth of insight into the horizontal transfer of resistance genes and add interpretive value to the genomic diversity and evolution of pPrY2001-like plasmids.

19.
J Colloid Interface Sci ; 557: 673-682, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31563058

RESUMO

Rational design of tin dioxide (SnO2) nanomaterials with superior architectures and outstanding physicochemical capabilities is highly desirable for gas sensors. Here, three SnO2 nanostructures with different morphologies, particles, core-shell spheres and facet-exposed crystals, are developed and further applied to track amounts of volatile organic compounds (VOCs). Porous SnO2 core-in-hollow-shell sphere-based sensors exhibited enhanced sensing properties, especially a higher sensitivity than SnO2 particles. The monocrystalline SnO2 single-crystal-based sensor, which has dominant exposed (1 1 0) and (2 2 1) facets, also showed a superior sensing performance, especially faster response/recovery speed than the SnO2 particle-based sensor. The enhanced gas-sensing properties are mainly ascribed to the structural sensitization, and these results further confirm that the SnO2 core-shell structure and exposed single crystal exposed with high energy can provide more numerous active sites for gas molecule adsorption than that of SnO2 particles.

20.
Clin Exp Pharmacol Physiol ; 46(11): 1053-1060, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31407376

RESUMO

Endothelial dysfunction is a precursor of cardiovascular disease, and oxidized low-density lipoprotein (ox-LDL) has been implicated in the development of atherosclerosis by directly targeting endothelial cells. Morin, a natural flavonol, has been shown to protect endothelial cells from dysfunction. The present study was designed to evaluate the effect of morin on ox-LDL-induced injury and to investigate the underlying molecular mechanisms in human umbilical vein endothelial cells (HUVECs). The results showed that morin alleviated ox-LDL-induced endothelial injury and promoted the viability of HUVECs exposed to ox-LDL. Morin significantly inhibited the oxidative stress induced by ox-LDL by inhibiting the production of reactive oxygen species and malondialdehyde, and downregulating the level of superoxide dismutase. Moreover, morin markedly attenuated the overexpressed mRNA levels of the inflammatory factors interleukin (IL)-1ß, IL-6, and the adhesion molecules ICAM-1 and VCAM-1 induced by exposure to ox-LDL. We found that morin attenuated ox-LDL-induced injury in HUVECs by inducing autophagy. The protective effects of morin against ox-LDL-induced injury were dramatically reversed by chloroquine phosphate (CQ) treatment. Furthermore, morin up-regulated the expression of p-AMPK and down-regulated the level of p-mTOR in HUVECs exposed to ox-LDL, and this was significantly reversed by the AMPK inhibitor Compound C (CC). Taken together, our results demonstrated that morin attenuates ox-LDL-mediated injury by inducing autophagy via activating AMPK signalling in HUVECs.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA