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1.
Methods Mol Biol ; 2597: 1-9, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36374409

RESUMO

Hindlimb suspension is a well-established rodent model of disuse-induced atrophy and is commonly used to simulate the effects of bed rest and space flight on humans. Over the decades, this method has undergone many changes to reduce the stress response on the animals and improve the reliability of the data. Here, we detail our method of performing hindlimb suspension in mice that minimizes stress, maximizes the replicability of the data, and uses space efficiently.


Assuntos
Elevação dos Membros Posteriores , Músculo Esquelético , Humanos , Camundongos , Animais , Elevação dos Membros Posteriores/efeitos adversos , Elevação dos Membros Posteriores/métodos , Reprodutibilidade dos Testes , Músculo Esquelético/patologia , Modelos Animais de Doenças , Roedores , Atrofia , Membro Posterior
2.
Vet Res ; 53(1): 93, 2022 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-36397147

RESUMO

An alphaherpesvirus carries dozens of viral proteins in the envelope, tegument and capsid structure, and each protein plays an indispensable role in virus adsorption, invasion, uncoating and release. After infecting the host, a virus eliminates unfavourable factors via multiple mechanisms to escape or suppress the attack of the host immune system. Post-translational modification of proteins, especially phosphorylation, regulates changes in protein conformation and biological activity through a series of complex mechanisms. Many viruses have evolved mechanisms to leverage host phosphorylation systems to regulate viral protein activity and establish a suitable cellular environment for efficient viral replication and virulence. In this paper, viral protein kinases and the regulation of viral protein function mediated via the phosphorylation of alphaherpesvirus proteins are described. In addition, this paper provides new ideas for further research into the role played by the post-translational modification of viral proteins in the virus life cycle, which will be helpful for understanding the mechanisms of viral infection of a host and may lead to new directions of antiviral treatment.


Assuntos
Alphaherpesvirinae , Animais , Fosforilação , Processamento de Proteína Pós-Traducional , Proteínas Virais/metabolismo , Replicação Viral
3.
Plant Cell Environ ; 2022 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-36368774

RESUMO

Intracellular immune receptor nucleotide-binding leucine-rich repeats (NLRs) are highly regulated transcriptionally and post-transcriptionally for balanced plant defence and growth. NLR genes often exist in gene clusters and are usually co-expressed under various conditions. Despite of intensive studies of regulation of NLR proteins, cis-acting elements for NLR gene induction, repression or co-expression are largely unknown due to a larger than usual cis-region for their expression regulation. Here we used the CRISPR/Cas9 genome editing technology to generate a series of in situ deletions at the endogenous location of a NLR gene SNC1 residing in the RPP5 gene cluster. These deletions that made in the wild type and the SNC1 constitutive expressing autoimmune mutant bon1 revealed both positive and negative cis-acting elements for SNC1 expression. Two transcription factors that could bind to these elements were found to have an impact on the expression of SNC1. In addition, co-expression of two genes with SNC1 in the same cluster is found to be mostly dependent on the SNC1 function. Therefore, SNC1 expression is under complex local regulation involving multiple cis elements and SNC1 itself is a critical regulator of gene expression of other NLR genes in the same gene cluster.

4.
J Agric Food Chem ; 2022 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-36444759

RESUMO

The intervention of cysteine (Cys) on the formation of 2,3-butanedione and pyrazines was evaluated during the thermal processing of the alanine-xylose Amadori compound (AX-ARP). With the involvement of Cys, the competitive formation of 2,3-butanedione and pyrazines was induced. The formation of 2,3-butanedione in the AX-ARP/Cys model was suppressed due to the inhibitory effect of the precursors of 2,3-butanedione like deoxypentosones, while the added Cys in the AX-ARP/Cys model competed with the recovered alanine (Ala) to capture glyoxal and methylglyoxal to make up for the absence of pyrazines in the AX-ARP model at an initial pH value of 7. The content of pyrazines increased from 0 up to 16.48 µg/L (120 °C, 120 min). Exogenous Cys itself showed lower reactivity with 2,3-butanedione through the Strecker degradation reaction; while the pH was increased to 8, the degradative products of Cys were facilitated to consume the residual 2,3-butanedione giving rise to the formation of 2,4,5-trimethylthiazole at 120 °C. It was the degradative products of Cys that accelerated the reaction for consumption of 2,3-butanedione rather than Cys itself. Additionally, the inhibitory effect of Cys on 2,3-butanedione formation was weakened under a basic condition, while the promotional effect on the formation of pyrazines was further boosted. With more Cys participating in the process of AX-ARP thermal degradation, the formation of 2,3-butanedione was further inhibited, while the yields of pyrazines were increased.

5.
Front Bioeng Biotechnol ; 10: 1068699, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36425650

RESUMO

Biological macromolecules have been widely used as biomedical carriers in treating non-small cell lung cancer (NSCLC) due to their biocompatibility, targeting, biodegradability, and antitumor efficacy. Nanotechnology has been used in clinics to treat many diseases, including cancer. Nanoparticles (NPs) can accumulate drugs into tumors because of their enhanced permeability and retention (EPR) effects. However, the lack of active targeting ligands affects NPs drug delivery. Arginine-glycine-aspartic (RGD), as a targeting ligand, has distinct advantages in targeting and safety. In the present study, an RGD peptide-modified nanogel called RGD-polyethylene glycol-poly (L-phenylalanine-co-L-cystine) (RGD-PEG-P (LP-co-LC-P (LP-co-LC) was investigated to deliver vincristine (VCR) as NSCLC therapy. The VCR-loaded targeted nanoparticle (RGD-NP/VCR) demonstrated excellent antitumor efficacy compared to the free drug (VCR) and untargeted nanoparticle (NP/VCR) without any significant side effects. RGD-NP/VCR has better tumor inhibition and fewer side effects, indicating its potential benefit in NSCLC treatment.

6.
EBioMedicine ; 86: 104340, 2022 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-36356476

RESUMO

BACKGROUND: Early detection of gastric cancer (GC) remains challenging. We aimed to examine urine proteomic signatures and identify protein biomarkers that predict the progression of gastric lesions and risk of GC. METHODS: A case-control study was initially designed, covering subjects with GC and gastric lesions of different stages. Subjects were aged 40-69 years, without prior diagnosis of renal or urological diseases. We enrolled a total of 255 subjects, with 123 in the discovery stage from Linqu, China, a high-risk area for GC and 132 in the validation stage from Linqu and Beijing. A prospective study was further designed for a subset of 60 subjects with gastric lesions, which were followed for 297-857 days. FINDINGS: We identified 43 differentially expressed urine proteins in subjects with GC vs. mild or advanced gastric lesions. Baseline urinary levels of ANXA11, CDC42, NAPA and SLC25A4 were further positively associated with risk of gastric lesion progression. Three of them, except for SLC25A4, also had higher expression in GC than non-GC tissues. Integrating these four proteins showed outstanding performance in predicting the progression of gastric lesions (AUC (95% CI): 0.92 (0.83-1.00)) and risk of GC (AUC (95% CI): 0.81 (0.73-0.89) and 0.84 (0.77-0.92) for GC vs. mild or advanced gastric lesions respectively). INTERPRETATION: This study revealed distinct urine proteomic profiles and a panel of proteins that may predict the progression of gastric lesions and risk of GC. These biomarkers in a non-invasive approach may have translational significance for defining high-risk populations of GC and its early detection. FUNDING: Funders are listed in the Acknowledgement.

7.
Immunol Lett ; 251-252: 29-37, 2022 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-36191776

RESUMO

BACKGROUND: Psoriasis is a chronic inflammatory disease. Mast cells are significantly increased and activated in the lesions of patients with psoriasis, contributing to psoriatic inflammation. Dermcidin (DCD) is a natural antibacterial peptide secreted by sweat glands and is usually transported to the epidermal surface by sweat. Whether DCD is involved in mast cell activation remains unclear and the mechanisms by which DCD is involved in skin inflammatory reactions require further investigation. METHODS: We investigated whether dermcidin-derived polypeptides DCD(86-103) activate mast cells and induce skin inflammatory reactions that contribute to psoriasis. Wild-type mice were treated with DCD(86-103) to observe the inflammatory reactions in the skin and cytokine release in vivo. The release of inflammatory mediators by mouse primary mast cells and LAD2 cells was measured in vitro. Molecular docking analysis, molecular dynamics simulation, and siRNA transfection were used to identify DCD(86-103). RESULTS: DCD(86-103) caused a skin inflammatory reaction in wild-type mice via cytokine release. Moreover, DCD(86-103) directly activated mast cells and induced cytokine release in vitro. ST2 may be a key receptor that mediates the activation effect of DCD(86-103) on mast cells leading to cytokine release. CONCLUSION: DCD(86-103) may have induced an inflammatory reaction and participated in the occurrence and development of psoriasis.

8.
Environ Sci Technol ; 56(22): 16024-16032, 2022 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-36239620

RESUMO

Endosulfan is an extensively used organochlorine pesticide around the world, which was classified as a persistent organic pollutant (POP) in 2009. Although previous studies have documented the reproductive toxicity of endosulfan in a variety of organisms, little is known about the influence of endosulfan on the genome stability of germ cells and nonexposed progeny. Here we applied whole-genome sequencing to explore the germ cell mutagenicity of α-endosulfan in Caenorhabditis elegans (C. elegans). We found that, although low doses of α-endosulfan exhibited a minor effect on the reproductive capacity of C. elegans, chronic exposure to 1 µM α-endosulfan significantly increased the mutation frequencies of nonexposed progeny. Further analysis of genome-wide mutation spectra demonstrated that α-endosulfan preferentially elicited A:T → G:C substitutions and clustered mutations. By using worms deficient in DNA damage response genes, our results suggest the involvement of translesion synthesis polymerase η in modulating α-endosulfan-induced mutations in germ cells. Together, these observations reveal the germ cell mutagenicity of α-endosulfan in C. elegans and the possible underlying mechanism. In addition, our findings implicate that germ cell mutagenicity might be a necessary consideration for the health risk assessment of environmental chemicals such as POPs.


Assuntos
Endossulfano , Praguicidas , Animais , Endossulfano/farmacologia , Endossulfano/toxicidade , Caenorhabditis elegans/genética , Mutagênicos/toxicidade , Células Germinativas , Praguicidas/toxicidade
9.
Environ Pollut ; 314: 120327, 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36195194

RESUMO

Cadmium (Cd) isotope fractionation patterns within soil profiles and the underlying mechanisms remain unclear and poorly documented. Here, Cd concentrations and isotope compositions of metal ore, surface soils and soil profile samples around a lead-zinc mine in southwest China were determined, and the relationships between soil properties and Cd isotope fractionation within the soil profiles were investigated. Cadmium concentrations of eleven surface soil samples were 0.49-66.1 mg kg-1 and the samples with high Cd concentrations had Cd isotope compositions similar to the metal ore (δ114/110Cd = 0.02‰), indicating that mining activity was the main Cd source at the study areas. Within three soil profiles with different Cd pollution levels the δ114/110Cd values gradually increased with increasing depth from 0 to 40 cm (Δ114/110Cd = 0.08-0.18‰), reaching a maximum at 30-40 cm depth, and then remained fairly constant or decreased with increasing soil depth below 40 cm. Soil δ114/110Cd values were negatively correlated with free iron and manganese oxides contents, which decreased at 0-40 cm depth then increased below 40 cm. This indicates that light Cd isotopes within 0-40 cm depth preferentially migrated downward with free iron and manganese oxides, leaving the soils at a depth of 0-40 cm enriched in heavy Cd isotopes. At 40-90 cm depth the preferential retention of heavy Cd isotopes by hydroxides may be responsible for the gradual decrease in δ114/110Cd values with increasing soil depth. These observations demonstrate that the vertical migration of Cd can induce detectable isotope fractionation within soil profiles and alter the δ114/110Cd values including those of the surface soils. Our study highlights the need to consider Cd mobilization and transport in soil profiles when tracing metal sources using isotope techniques.


Assuntos
Poluentes do Solo , Solo , Cádmio/análise , Manganês , Isótopos/análise , Poluentes do Solo/análise , Zinco/análise , Ferro , Óxidos , China , Monitoramento Ambiental/métodos
10.
Development ; 149(21)2022 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-36227586

RESUMO

High-resolution ribosome fractionation and low-input ribosome profiling of bovine oocytes and preimplantation embryos has enabled us to define the translational landscapes of early embryo development at an unprecedented level. We analyzed the transcriptome and the polysome- and non-polysome-bound RNA profiles of bovine oocytes (germinal vesicle and metaphase II stages) and early embryos at the two-cell, eight-cell, morula and blastocyst stages, and revealed four modes of translational selectivity: (1) selective translation of non-abundant mRNAs; (2) active, but modest translation of a selection of highly expressed mRNAs; (3) translationally suppressed abundant to moderately abundant mRNAs; and (4) mRNAs associated specifically with monosomes. A strong translational selection of low-abundance transcripts involved in metabolic pathways and lysosomes was found throughout bovine embryonic development. Notably, genes involved in mitochondrial function were prioritized for translation. We found that translation largely reflected transcription in oocytes and two-cell embryos, but observed a marked shift in the translational control in eight-cell embryos that was associated with the main phase of embryonic genome activation. Subsequently, transcription and translation become more synchronized in morulae and blastocysts. Taken together, these data reveal a unique spatiotemporal translational regulation that accompanies bovine preimplantation development.


Assuntos
Blastocisto , Desenvolvimento Embrionário , Gravidez , Feminino , Bovinos , Animais , Desenvolvimento Embrionário/genética , Mórula/metabolismo , Blastocisto/metabolismo , Oócitos/metabolismo , Ribossomos/genética , Regulação da Expressão Gênica no Desenvolvimento
11.
Bioresour Technol ; 365: 128091, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36257522

RESUMO

Although mainstream partial nitrification-anammox (PN-A) is a highly efficient and sustainable wastewater treatment process, it is difficult to achieve and stabilize due to the competition among functional bacteria. In this study, achieving one-stage mainstream anammox via regulating bacteria community structure was studied in a lab-scale biological aerated filter (BAF). The results showed that high free ammonia with 89.57 mg/L, nitrite nitrogen (NO2--N) competition between anammox bacteria (AnAOB) and nitrite oxidizing bacteria (NOB), and backwash regulated the bacteria community structure. After backwash, Candidatus Kuenenia became the dominant bacteria and the relative abundance increased to 5.56 %. In BAF, one-stage mainstream anammox with total nitrogen (TN) being lower than 15 mg/L in the effluent was achieved using lag-time of bacteria activity recovery caused by alternating operation of high and low ammonia nitrogen (NH4+-N), which have great potential applied in municipal wastewater treatment plants (MWWTPs).


Assuntos
Desnitrificação , Nitritos , Reatores Biológicos/microbiologia , Amônia , Oxidação Anaeróbia da Amônia , Nitrogênio/química , Bactérias , Oxirredução , Águas Residuárias/química , Esgotos/microbiologia
12.
Ann Palliat Med ; 11(9): 3014-3019, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-36217629

RESUMO

BACKGROUND: Arteriovenous fistula is the lifeline of maintenance for patients requiring hemodialysis, with thrombosis being a common complication of this procedure. Traditionally, thrombi have been removed via thrombectomy. In recent years, it has been reported that newly occurring thrombi can be treated by urokinase thrombolysis. However, the thrombus shedding in the process should be valued, which may cause the distal limb ischemia syndrome. The complication of thrombolysis is rare but serious. Patients will experience pain and numbness, and possibly even extremity necrosis may occur without diagnosed or treated timely. There are not any reports about the occurrence or treatment of distal limb ischemia syndrome caused by thrombus shedding during thrombolysis. CASE DESCRIPTION: Considering the thrombosis volume and texture of this case, we attempted to use urokinase thrombolysis to resolve the thrombus in fistula. During thrombolysis, thrombus shedding occurred in the distal limb. The patient's fingers of the limbs on the side of the internal fistula were pale, numb, and painful in the case. Fortunately, we solved the problem ultimately by continuously pumping urokinase. The heparin, urokinase, infusion pumps, ultrasound, and infrared therapy devices were obtained from the Affiliated Hospital of Chuanbei Medical College. Their usage and dosage are described in the relevant literature and China's 2020 blood purification standard operating procedures. CONCLUSIONS: In the process of thrombolysis of arteriovenous fistula, attention should be paid to thrombus shedding. Distal limb ischemia syndrome is a rare but serious complication of thrombus shedding. Continued pumping of urokinase may be effective for this complication.


Assuntos
Fístula Arteriovenosa , Trombose , Fístula Arteriovenosa/terapia , Heparina/uso terapêutico , Humanos , Isquemia/etiologia , Isquemia/terapia , Terapia Trombolítica/métodos , Trombose/tratamento farmacológico , Trombose/etiologia , Resultado do Tratamento , Ativador de Plasminogênio Tipo Uroquinase/uso terapêutico
13.
Front Aging Neurosci ; 14: 985406, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36247990

RESUMO

Objective: Preoperative levels of cognition-related biomarkers and intraoperative cerebral ischemia and hypoxia might cause postoperative neurocognitive dysfunction (PND). The aim of this study was to evaluate the predictive ability of preoperative plasma biomarkers along with cerebral oxygen saturation (SctO2) for the incidence of PND in elderly patients with mild cognitive impairment (MCI). Methods: A total of 210 patients aged 65-80 years undergoing spinal surgery were randomly assigned to three groups (n = 70 each): propofol, sevoflurane, and propofol/sevoflurane as anesthesia maintenance protocols. Propofol was administrated target-controlled infusion of 4 µg/ml (group P), the minimum alveolar concentration (MAC) of inhalation anesthetic sevoflurane was 1.3 (group S), and propofol was injected with a target-controlled plasma concentration of 1.2 µg/ml, accompanied by sevoflurane inhalation 0.7 MAC (group PS). Cognitive function was evaluated 1 day preoperatively and on the 7th day postoperatively. Preoperative levels of amyloidß-40 (Aß-40), Aß-42, total tau protein (T-tau), phosphorylated tau protein (P-tau), and triggering receptors on myeloid cells-2 (TREM2) were investigated. SctO2 was monitored intraoperatively. Results: Aß-42 had the strongest significant correlation with preoperative MoCA score. The value of Aß-42 associated with a high risk of PND was 28.34 pg/ml, and the area under the curve (AUC) was predicted to be 0.711. When the preoperative level of Aß-42 was 28.34 pg/ml, SctO2max% was 9.92%. The AUC was predicted to be 0.872, and the sensitivity and specificity were 0.833 and 0.841, respectively. Conclusion: Under the conditions of preoperative Aß-42 less than 28.34 pg/ml, the intraoperative fluctuation range of cerebral oxygen saturation should be maintained within 9.92% to reduce the occurrence of PND in geriatric patients with MCI.

14.
Int J Mol Sci ; 23(19)2022 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-36233293

RESUMO

Thyroid cancer remains the most common endocrine malignancy worldwide, and its incidence has steadily increased over the past four years. Papillary Thyroid Cancer (PTC) is the most common differentiated thyroid cancer, accounting for 80-85% of all thyroid cancers. Mitochondrial proteins (MRPs) are an important part of the structural and functional integrity of the mitochondrial ribosomal complex. It has been reported that MRPL9 is highly expressed in liver cancer and promotes cell proliferation and migration, but it has not been reported in PTC. In the present study we found that MRPL9 was highly expressed in PTC tissues and cell lines, and lentivirus-mediated overexpression of MRPL9 promoted the proliferation and migration ability of PTC cells, whereas knockdown of MRPL9 had the opposite effect. The interaction between MRPL9 and GGCT (γ-glutamylcyclotransferase) was found by immunofluorescence and co-immunoprecipitation experiments (Co-IP). In addition, GGCT is highly expressed in PTC tissues and cell lines, and knockdown of GGCT/MRPL9 in vivo inhibited the growth of subcutaneous xenografts in nude mice and inhibited the formation of lung metastases. Mechanistically, we found that knockdown of GGCT/MRPL9 inhibited the MAPK/ERK signaling pathway. In conclusion, our study found that the interaction of GGCT and MRPL9 modulates the MAPK/ERK pathway, affecting the proliferation and migration of PTC cells. Therefore, GGCT/MRPL9 may serve as a potential biomarker for PTC monitoring and PTC treatment.


Assuntos
Sistema de Sinalização das MAP Quinases , Neoplasias da Glândula Tireoide , gama-Glutamilciclotransferase , Animais , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células/genética , Regulação Neoplásica da Expressão Gênica , Humanos , Camundongos , Camundongos Nus , Proteínas Mitocondriais/genética , Proteínas Mitocondriais/metabolismo , Câncer Papilífero da Tireoide/patologia , Neoplasias da Glândula Tireoide/patologia , gama-Glutamilciclotransferase/genética
15.
Acta Biomater ; 2022 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-36210045

RESUMO

Hypoxic nonhealing wounds are a common complication in chronic patients, and chronic hypoxia is the main reason for delayed wound healing, so local wound oxygenation may be an effective way to address this problem. Here, we proposed a system consisting of oxygen-releasing microsphere (GC) and self-healing hydrogel (QGO). QGO/GC hydrogel could promote survival, migration and tube formation of human umbilical vein endothelial cells under hypoxic conditions. Moreover, QGO/GC hydrogels exhibited biocompatibility in vitro and in vivo. The hypoxic mouse burn model further confirmed that QGO/GC hydrogel could promote tissue repair by reducing inflammation (TNF-α and IL-1ß), increasing angiogenesis (CD31, VEGF and α-SMA) and collagen deposition. This study provided an effective oxygen-releasing hydrogel that could offer a simple and effective method for the clinical treatment of chronic hypoxic wounds. STATEMENT OF SIGNIFICANCE: Burn injury is caused by various exogenous factors such as friction, cold, radiations, electricity, chemicals, hot surfaces or liquids. Severe burn can damage the entire skin layer, and the healing process is delayed due to an unbalanced inflammatory response, excessive reactive oxygen species, lack of angiogenesis (insufficient nutrient and oxygen availability), and susceptibility to infection. In the present study, we proposed an oxygen-releasing hydrogel (QGO/GC). QGO/GC hydrogel could promote survival, migration, and tube formation of human umbilical vein endothelial cells under hypoxic conditions. And QGO/GC hydrogels could promote tissue repair by reducing inflammation, increasing angiogenesis and collagen deposition. This work provided an effective oxygen-releasing hydrogel for the clinical management of chronic hypoxic wounds.

16.
J Environ Manage ; 324: 116336, 2022 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-36162317

RESUMO

Particulate cadmium (Cd) and zinc (Zn) are ubiquitous in agricultural soils of Pb-Zn mining regions. Water management serves as an important agronomic measure altering the bioavailability of Zn and Cd in soils, but how this affects particulate Cd and Zn and the underlying mechanisms remain largely unknown. Microcosm soil incubation combined with spectroscopic and microscopic characterization was conducted. During a two-year-long incubation period we observed that the concentrations of soil CaCl2-extractable Zn and Cd increased 3-10 times in sphalerite-spiked soils and 1-2 times in smithsonite-spiked soils under periodic flooding conditions due to the long-term dissolution of sphalerite (SP) and smithsonite (SM). However, the increase in the concentration of CaCl2-extractable metals (Zn: from 0.607 mg kg-1 to 1.051 mg kg-1 and Cd: from 0.047 mg kg-1 to 0.119 mg kg-1) was found only in SP-treatment under continuous flooding conditions, indicating the mobilization of metals. Ultrafiltration analysis shows that the nanoparticulate fraction of Zn and Cd in soil pore water increased 5 and 7 times in SP-treatments under continuous flooding conditions, suggesting the increment of metal pools in soil pore water. HRTEM-EDX-SAED further reveals that these nanoparticles were mainly crystalline ZnS and Zn-bearing sulfate nanoparticles in the SP-treatment and amorphous ZnCO3 and ZnS nanoparticles in the SM-treatment. Therefore, the formation of the stable crystalline Zn-bearing nanoparticles in the SP-treatment may explain the elevation of the concentration of soil CaCl2-extractable Zn and Cd under continuous flooding. The potential mobility of particulate metals should therefore be expected in scenarios of continuous flooding such as paddy soils and wetland systems.


Assuntos
Metais Pesados , Poluentes do Solo , Cádmio/química , Solo/química , Zinco/química , Poluentes do Solo/análise , Água/análise , Cloreto de Cálcio , Ácidos , Abastecimento de Água , Metais Pesados/análise
17.
Chemosphere ; 308(Pt 3): 136589, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36162513

RESUMO

Chemical weathering of carbonate-hosted Pb-Zn mines via acid-promoted or oxidative dissolution generates metal-bearing colloids at neutral mine drainage sites. However, the mobility and bioavailability of the colloids associated with metals in nearby soils are unknown. Here, we monitored the mobility of metal(loid)s in soils affected by aeolian deposition and river transport in the vicinity of a carbonate-hosted Pb-Zn mine. Using chemical extraction, ultrafiltration, and microscopic and spectroscopic analysis of metals we find that contamination levels of the soil metals cadmium (Cd), lead (Pb) and zinc (Zn) were negatively correlated with metal extractability. However, nano-scale characterization indicates that colloid-metal(loid) interactions induced potential mobilization and increased risk from metal(loid)s. Dynamic light scattering (DLS) and HRTEM-EDX-SAED analysis further indicate that organic matter (OM)-rich nano-colloids associated with calcium (Ca), silicon (Si) and iron (Fe) precipitates accounted for the majority of the dissolved metal fractions in carbonate-hosted Pb-Zn mine soils. More stable nano-crystals (ZnS, ZnCO3, Zn-bearing sulfates, hematite and Al-Si-Fe compounds) were present in the pore water of aeolian-impacted upland soils rather than in river water-impacted soils. Our results suggest that future work should consider the possibility that potential mobilization of metal(loid)s induced by the weathering and transformation of these metal-bearing nano-crystals to metal-bearing amorphous colloids, potentially elevating metal mobility and/or bioavailability in river water-impacted agricultural soils.


Assuntos
Metais Pesados , Poluentes do Solo , Cádmio/análise , Cálcio/análise , Carbonatos/análise , Monitoramento Ambiental/métodos , Ferro/análise , Chumbo/análise , Metais Pesados/análise , Silício/análise , Solo , Poluentes do Solo/análise , Sulfatos/análise , Água/análise , Zinco/análise
18.
J Affect Disord ; 319: 252-259, 2022 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-36155231

RESUMO

BACKGROUND: The adverse impact of depressive symptoms on adolescents' physical and psychological health is becoming increasingly prominent. This study aims to examine the association between sleep duration, cognitive function, and depressive symptoms among Chinese adolescents. METHODS: This study used data on a sample of 3724 adolescents collected from the China Family Panel Studies (CFPS). The multivariate logistic regression was conducted to estimate the association between sleep duration, cognitive function (measured by mathematics and vocabulary), and depressive symptoms among Chinese adolescents; the mediating effects of cognitive function were calculated by mediating model. RESULTS: The study found that approximately 6.49 % of Chinese adolescents had depressive symptoms, with an average CES-D8 score of 4.27 (SD: 3.11). Adolescents who reported a sleep duration of <6 h/night (OR = 2.34; 95 % CI = 1.30, 4.24) were at a higher risk of depressive symptoms, and the adolescents who had higher mathematics scores (>75 %) were more likely to be at a lower risk of depressive symptoms. Besides, the cognitive function competitively mediated the effect of sleep duration on depressive symptom (indirect effect = 0.043; 95 % CI = 0.023, 0.064). LIMITATIONS: This is a cross-sectional study; all questions were self-reported; the sleep variable only included the sleep duration. CONCLUSIONS: Our study highlights that short sleep duration might increase the risk of depressive symptoms, and increasing cognitive function might reduce the occurrence of depressive symptoms in Chinese adolescents. The results displayed that sleep duration had a negatively direct effect on depressive symptoms, but the indirect effects of sleep duration on depressive symptoms via the cognitive function (i.e., mathematics and vocabulary tests) were significantly positive, indicating that the relationship between sleep duration and depressive symptoms was competitively mediated by cognitive function.


Assuntos
Depressão , Sono , Adolescente , Humanos , Depressão/epidemiologia , Depressão/psicologia , Estudos Transversais , China/epidemiologia , Cognição
19.
Acta Biomater ; 153: 585-595, 2022 11.
Artigo em Inglês | MEDLINE | ID: mdl-36167235

RESUMO

As an alternative strategy to achieve the desired bone augmentation, tenting screw technology (TST) has considerably broadened the indications for implant treatment. Titanium tenting screws are typically used in TST to maintain the space for bone regeneration. However, a high degree of osteogenic integration complicate titanium tenting screw removal and impact the bone healing micro-environment. Previous efforts have been focused on modifying titanium surfaces to enhance osseointegration while ignoring the opposite process. Due to the vital role of bone marrow mesenchymal stem cells (BMSCs) in bone regeneration, it might be feasible to reduce osseointegration around titanium tenting screws by resisting the adhesion of BMSCs. Herein, poly(ethylene glycol)methyl ether methacrylate (poly(PEGMA)) with an optimal length of PEG chain was incorporated with a Ti surface in terms of surface-initiated activators regenerated by electron transfer atom transfer radical polymerization (SI-ARGET ATRP). The cell apoptosis analysis showed that the new surface would not induce the apoptosis of BMSCs. Then, the adhesive and proliferative behaviors of BMSCs on the surface were analyzed which indicated that the poly(PEGMA) surface could inhibit the proliferation of BMSCs through resisting the adhesion process. Furthermore, in vivo experiments revealed the presence of the poly(PEGMA) on the surface resulted in a lower bone formation and osseointegration compared with the Ti group. Collectively, this dense poly(PEGMA) surface of Ti may serve as a promising material for clinical applications in the future. STATEMENT OF SIGNIFICANCE: The poly(ethylene glycol)methyl ether methacrylate (poly(PEGMA)) with an optimal length of PEG chain was grafted onto a Ti surface by surface-initiated activators regenerated by electron transfer atom transfer radical polymerization (SI-ARGET ATRP). The PEGMA surface could reduce the osteogenic integration by preventing the adhesion of cells, resulting in a lower pullout force of the modified implant and thereby desirable and feasible applications in dental surgery.


Assuntos
Incrustação Biológica , Células-Tronco Mesenquimais , Éteres Metílicos , Osseointegração , Titânio/farmacologia , Incrustação Biológica/prevenção & controle , Metacrilatos/farmacologia , Metacrilatos/metabolismo , Polietilenoglicóis/farmacologia , Polietilenoglicóis/metabolismo , Éteres Metílicos/metabolismo , Propriedades de Superfície , Células da Medula Óssea/metabolismo
20.
Front Public Health ; 10: 980465, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36062100

RESUMO

Objective: Disability and social support can impact depressive symptoms of the elderly. Yet, studies infrequently discuss the moderating role of social support when evaluating the association between disability and depressive symptoms. The purpose of this study was to explore the association between disability, social support, and depressive symptoms among the Chinese elderly, and further examine the moderating effect of social support. Materials and methods: Using the 2018 Chinese Longitudinal Healthy Longevity Survey (CLHLS) data set, we finally selected 9,231 Chinese elderly after screening. The Center for Epidemiologic Studies Depression Scale (CESD-10) was used to evaluate depressive symptoms in the elderly. Disability was measured by basic activities of daily living (B-ADL) and instrumental activities of daily living (I-ADL). Social support included contact with family and friends, sick care, and money received, measured by five self-reported questions. We used multiple linear regression and moderating model to explore the association between disability, social support, and depressive symptoms. Results: A total of 9,231 patients were included in this study, and approximately 26.75% of the elderly had depressive symptoms. Study found that depressive symptoms were associated with social support (ß B-ADL = -0.108, 95% CI: -0.168- -0.047; ß I-ADL = -0.098, 95% CI: -0.156- -0.039), ß B-ADL (ß = 0.296, 95% CI: 0.248-0.343) and I-ADL (ß = 0.174, 95% CI: 0.152-0.195). Moreover, the result also showed that social support moderated the effects of B-ADL ( ß B - A D L * s o c i a l s u p p o r t = 0.034, 95% CI: 0.014-0.053, F = 11.57, p = 0.001) and I-ADL ( ß I - A D L * s o c i a l s u p p o r t = 0.025, 95% CI: 0.017-0.033) on depressive symptoms. Conclusions: The study suggests that disability and social support can affect depressive symptoms, and social support moderates the effect of disability on depressive symptoms. Therefore, taking effective measures to reduce the elderly disability rate of disability and increase their social support are necessary condition for realizing mental health.


Assuntos
Atividades Cotidianas , Pessoas com Deficiência , Atividades Cotidianas/psicologia , Idoso , China/epidemiologia , Depressão/epidemiologia , Depressão/psicologia , Pessoas com Deficiência/psicologia , Humanos , Apoio Social
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