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1.
Z Gerontol Geriatr ; 2019 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-31435788

RESUMO

Previous studies have demonstrated the damaging effect of alcohol (ALH) consumption on bone tissue and bone metabolism. Resveratrol (RES) promotes osteoblast proliferation and inhibits osteoclast proliferation and positively affects bone regeneration; however, reports about effects of RES on osseointegration in aged female rats with ALH consumption are limited. This study was designed to investigate the impact of treatment with RES on osseointegration for aged female rats with ALH consumption. This study included 30 female Sprague-Dawley rats (22 months old), weighing approximately 520 g. All animals were randomly divided into 3 groups of 10: a control group (CON) receiving saline, a group receiving ALH and a group receiving ALH + RES until death after 12 weeks. The results of enhanced osseointegration in senile female rats with RES consumption were evaluated by histology, microcomputerized tomography (micro-CT), gene expression analysis and a biomechanical test. The results of this study indicated that ALH + RES showed stronger effects on the improvement of osseointegration in senile female rats with ALH consumption. The ALH + RES produced stronger effects on bone volume per total volume (BV/TV), mean trabecular thickness (Tb.Th), mean trabecular number (Tb.N) and mean trabecular separation (Tb.Sp), connective tissue density (Conn.D) and maximum push-out force for implants, and regulation of osteogenesis and bone resorption-related gene expression. These results seem to indicate that RES intervention reverses the negative effect of alcohol on hydroxyapatite-coated implant osseointegration in senile female rats with ALH consumption.

2.
J Bone Miner Metab ; 2019 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-31076895

RESUMO

Composite materials ß-tricalcium phosphate (ß-TCP) and poly-lactic-co-glycolic acid (PLGA) have achieved stable bone regeneration without cell transplantation in previous studies. Recent research shows that aspirin (ASP) has great potential in promoting bone regeneration. The objective of the present study was to incorporate PLGA into ß-TCP combined with a lower single-dose local administration of ASP to enhance its in vivo biodegradation and bone tissue growth. After the creation of a rodent critical-sized femoral metaphyseal bone defect, PLGA -modified ß-TCP (TP) was prepared by mixing sieved granules of ß-TCP and PLGA (50:50, v/v) for medical use, then TP with dripped 50 µg/0.1 ml and 100 µg/0.1 ml aspirin solution was implanted into the defect of OVX rats until death at 8 weeks. The defected area in distal femurs of rats was harvested for evaluation by histology, micro-CT, biomechanics and real time RT-PCR. The results of our study show that a single-dose local administration of ASP combined with the local usage of TP can increase the healing of defects in OVX rats. Single-dose local administration of aspirin can improve the transcription of genes involved in the regulation of bone formation and vascularization in the defect area, and inhibits osteoclast activity. Furthermore, treatments with a higher single-dose local administration of ASP and TP showed a stronger effect on accelerating the local bone formation than while using a lower dose of ASP. The results from our study demonstrate that the combination of a single-dose local administration of ASP and ß-TCP/PLGA had an additive effect on local bone formation in osteoporosis rats, and bone regeneration by PLGA/ß-TCP/ASP occured in a dose-dependent manner.

3.
J Bone Miner Metab ; 2018 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-29392472

RESUMO

Parathyroid hormone (1-34, PTH) combined ß-tricalcium phosphate (ß-TCP) achieves stable bone regeneration without cell transplantation in previous studies. Recently, with the development of tissue engineering slow release technology, PTH used locally to promote bone defect healing become possible. This study by virtue of collagen with a combination of drugs and has a slow release properties, and investigated bone regeneration by ß-TCP/collagen (ß-TCP/COL) with the single local administration of PTH. After the creation of a rodent critical-sized femoral metaphyseal bone defect, ß-TCP/COL was prepared by mixing sieved granules of ß-TCP and atelocollagen for medical use, then ß-TCP/COL with dripped PTH solution (1.0 µg) was implanted into the defect of OVX rats until death at 4 and 8 weeks. The defected area in distal femurs of rats was harvested for evaluation by histology, micro-CT, and biomechanics. The results of our study show that single-dose local administration of PTH combined local usage of ß-TCP/COL can increase the healing of defects in OVX rats. Furthermore, treatments with single-dose local administration of PTH and ß-TCP/COL showed a stronger effect on accelerating the local bone formation than ß-TCP/COL used alone. The results from our study demonstrate that combination of single-dose local administration of PTH and ß-TCP/COL had an additive effect on local bone formation in osteoporosis rats.

4.
Z Gerontol Geriatr ; 2018 Feb 23.
Artigo em Inglês | MEDLINE | ID: mdl-29476205

RESUMO

Recently, the use of the pharmacological agents strontium ranelate (SR), parathyroid hormone (1-34, PTH) and zoledronic acid (ZA) has come to prominence for the treatment of osteoporosis due to their ability to prevent bone loss in osteoporotic patients. Although much emphasis has been placed on using pharmacological agents for the prevention of disease, much less attention has been placed on which one is more effective. There is still no direct comparative study on these three drugs. The aim of the present study was to investigate the effect of SR, PTH, ZA on preventing ovariectomy-induced osteoporosis in rats. After bilateral ovariectomy the rats randomly received vehicle, SR (500 mg/kg body weight/day, orally), PTH (20 µg/kg/day, subcutaneously) or a single injection of ZA (0.1 mg/kg, i.v.) until death at 12 weeks. The distal femurs were harvested for evaluation of bone metabolism. The rats treated with ZA demonstrated the highest levels of new bone formation as assessed by microcomputed tomography (CT), biomechanical strength, histological analysis and bone metabolism. Furthermore, PTH and SR showed a stronger effect on improving trabecular bone mass at 12 weeks. The results from the present study demonstrate that systemic administration of PTH, SR and ZA could prevent bone loss, while a single dose of ZA has a better effect on preventing ovariectomy-induced osteoporosis than either PTH or SR.

5.
Mater Sci Eng C Mater Biol Appl ; 62: 226-32, 2016 May.
Artigo em Inglês | MEDLINE | ID: mdl-26952418

RESUMO

Surface modification techniques have been applied to generate titanium implant surfaces that promote osseointegration for the implants in cementless arthroplasty. However, its effect is not sufficient for osteoporotic bone. Zinc (Zn), magnesium (Mg), and strontium (Sr) present a beneficial effect on bone growth, and positively affect bone regeneration. The aim of this study was to confirm the different effects of the fixation strength of Zn, Mg, Sr-substituted hydroxyapatite-coated (Zn-HA-coated, Mg-HA-coated, Sr-HA-coated) titanium implants via electrochemical deposition in the osteoporotic condition. Female Sprague-Dawley rats were used for this study. Twelve weeks after bilateral ovariectomy, all animals were randomly divided into four groups: group HA; group Zn-HA; group Mg-HA and group Sr-HA. Afterwards, all rats from groups HA, Zn-HA, Mg-HA and Sr-HA received implants with hydroxyapatite containing 0%, 10% Zn ions, 10% Mg ions, and 10% Sr ions. Implants were inserted bilaterally in all animals until death at 12 weeks. The bilateral femurs of rats were harvested for evaluation. All treatment groups increased new bone formation around the surface of titanium rods and push-out force; group Sr-HA showed the strongest effects on new bone formation and biomechanical strength. Additionally, there are significant differences in bone formation and push-out force was observed between groups Zn-HA and Mg-HA. This finding suggests that Zn, Mg, Sr-substituted hydroxyapatite coatings can improve implant osseointegration, and the 10% Sr coating exhibited the best properties for implant osseointegration among the tested coatings in osteoporosis rats.


Assuntos
Materiais Revestidos Biocompatíveis/química , Durapatita/química , Magnésio/química , Estrôncio/química , Titânio/química , Zinco/química , Animais , Materiais Revestidos Biocompatíveis/toxicidade , Feminino , Fêmur/diagnóstico por imagem , Fêmur/patologia , Teste de Materiais , Microscopia Eletrônica de Varredura , Osseointegração/efeitos dos fármacos , Osteoporose/metabolismo , Osteoporose/patologia , Próteses e Implantes , Ratos , Ratos Sprague-Dawley , Espectrofotometria Atômica , Propriedades de Superfície , Microtomografia por Raio-X
6.
J Mater Sci Mater Med ; 27(3): 43, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26758890

RESUMO

The effect of human parathyroid hormone 1-34 (PTH) and simvastatin (SIM) alone could promote bone healing in osteoporotic implant fixation, but there are no reports about the combined use of PTH and SIM for promotion of bone healing around implant in osteoporotic settings. This study aims to investigate effects of PTH + SIM on implant stabilization in osteopenic rats. Fourteen weeks after chronically fed a low protein diet, osteopenic rats randomly received implants. Subsequently, the animals were randomly divided into four groups: Control, SIM, PTH and PTH + SIM. Then all rats from groups PTH, SIM and PTH + SIM received PTH (40 µg/kg, three times a week), SIM (25 mg/kg, daily), or both for 12 weeks. The results of our study indicated that all treatments promoted bone healing around implant compared to Control, but PTH + SIM treatment showed significantly stronger effects than PTH or SIM alone in histological, micro-CT, and biomechanical tests. The results indicated additive effects of PTH and SIM on implant fixation in osteoporotic rats.


Assuntos
Artroplastia de Quadril/instrumentação , Doenças Ósseas Metabólicas/patologia , Materiais Revestidos Biocompatíveis/farmacologia , Hormônio Paratireóideo/farmacologia , Sinvastatina/farmacologia , Titânio , Animais , Conservadores da Densidade Óssea/farmacologia , Regeneração Óssea/efeitos dos fármacos , Proteínas na Dieta/administração & dosagem , Feminino , Fêmur/patologia , Prótese de Quadril , Inibidores de Hidroximetilglutaril-CoA Redutases/administração & dosagem , Inibidores de Hidroximetilglutaril-CoA Redutases/farmacologia , Implantes Experimentais , Osseointegração/efeitos dos fármacos , Hormônio Paratireóideo/administração & dosagem , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Sinvastatina/administração & dosagem
7.
J Biomater Appl ; 30(7): 952-60, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26482573

RESUMO

Previous studies have demonstrated the effect of human parathyroid hormone (1-34) (PTH) or strontium-doped hydroxyapatite coating (Sr-HA) on osteoporotic bone implantation. However, reports about effects of PTH plus Sr-HA on bone osseointegration of titanium implants in a state of osteoporosis were limited. This study was designed to investigate the effects of intermittent administration of human parathyroid hormone (1-34) on strontium-doped hydroxyapatite coating (Sr-HA) implant fixation in ovariectomized (OVX) rats. Twelve weeks after bilateral ovariectomy, all animals were randomly divided into four groups including control group, Sr group, PTH group and PTH+Sr group. Forty OVX rats accepted implant insertion in the distal femurs, control group, and PTH group with HA implants and the Sr group and PTH+Sr group with Sr-HA implants. Animals from PTH group and PTH+Sr group then randomly received PTH (60 µg/kg, 3 times a week) until death at 12 weeks. After 12-week healing period, implants from group PTH+Sr revealed improved osseointegration compared with other treatment groups, which is manifested by the exceeding increase of bone area ratio and bone-to-implant contact, the trabecular microarchitecture and the maximal push-out force displayed by tests like histomorphometry, micro-CT, and biomechanics evaluation. These results demonstrated that PTH+ Sr-HA coatings could enhance implant osseointegration in OVX rats, and suggested the feasibility of using this method to improve implant fixation in osteoporotic bone.


Assuntos
Substitutos Ósseos/química , Durapatita/química , Fêmur/patologia , Hidroxiapatitas/química , Hormônio Paratireóideo/administração & dosagem , Estrôncio/química , Titânio/química , Animais , Fenômenos Biomecânicos , Conservadores da Densidade Óssea/química , Osso e Ossos/patologia , Materiais Revestidos Biocompatíveis/química , Eletroquímica , Feminino , Humanos , Teste de Materiais , Osseointegração , Osteoporose/tratamento farmacológico , Osteoporose/fisiopatologia , Hormônio Paratireóideo/metabolismo , Ratos , Ratos Sprague-Dawley , Estresse Mecânico , Microtomografia por Raio-X
8.
J Craniomaxillofac Surg ; 43(10): 2136-43, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26507646

RESUMO

The objective of this study was to evaluate the effect of following combined treatment with parathyroid hormone (1-34) (PTH) and ß-tricalcium phosphate (ß-TCP) on local bone formation in a rat 3-mm critical-sized defect at the distal femur. Fourteen weeks were allowed to pass before defect surgery for the establishment of osteopenic animal models chronically fed a low-protein diet. All animals were randomly divided into four groups: group PTH; group ß-TCP, group PTH + ß-TCP, and a control group. All rats then underwent a surgical procedure to create bone defects in the bilateral distal femurs, and ß-TCP was implanted into critical-sized defects for the groups designated as ß-TCP and group PTH + ß-TCP. After the defect operation, all animals from group PTH and group PTH + ß-TCP received following subcutaneous injections with PTH (60 µg/kg, three times per week) until euthanasia at 4 and 8 weeks. The distal femurs and blood were collected for evaluation. The results of study showed the strongest effect on accelerating the local bone formation with treatment ß-TCP and PTH at 4 weeks and 8 weeks. The results from our study demonstrate that a combination of PTH and ß-TCP had an additive effect on local bone formation in osteopenic rats chronically fed a low-protein diet.


Assuntos
Fosfatos de Cálcio/farmacologia , Fêmur/crescimento & desenvolvimento , Osteogênese/efeitos dos fármacos , Hormônio Paratireóideo/farmacologia , Animais , Ratos
9.
Injury ; 46(11): 2164-9, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26404665

RESUMO

The effect of human parathyroid hormone 1-34 (PTH) and simvastatin (SIM) alone could promote bone healing in osteoporotic osseous integration of the implant, but there are no reports about the combined use of PTH and SIM for promotion of bone healing around implant in osteoporotic settings still limited. This study aims to investigate effects of PTH+SIM on osseous integration of the implant in OVX rats. Female Sprague-Dawley rats were used for this study. Twelve weeks after bilateral ovariectomy, all animals were randomly divided into four groups: group control; group SIM; group PTH and group PTH+SIM. Afterwards, all OVX rats received hydroxyapatite (HA)-coated titanium rods (external diameter and length are 1.5mm and 20mm) in the femoral medullary canal. Subsequently, the animals from group SIM, group PTH and group PTH+SIM received human parathyroid hormone 1-34 (60µg/kg, three times a week), SIM (5mg/kg daily), or both for 12 weeks. Implants were inserted bilaterally in all animals until death at 12 weeks. The bilateral femurs of rats were harvested for evaluation. All groups increased new bone formation around the surface of titanium rods and push-out force; group PTH+SIM showed the strongest effects on new bone formation and biomechanical strength. Additionally, these are significant difference observed in bone formation and push-out force between groups SIM and PTH. This finding suggests that intermittent administration of PTH or SIM alone has an effect to increase new bone formation on the surface of HA-coated implants in the osteoporotic condition, and the additive effects of combination PTH and SIM on osseous integration of the implant in OVX rats.


Assuntos
Artroplastia de Quadril/instrumentação , Conservadores da Densidade Óssea/farmacologia , Materiais Revestidos Biocompatíveis/farmacologia , Fêmur/patologia , Osseointegração/efeitos dos fármacos , Osteoporose/patologia , Hormônio Paratireóideo/farmacologia , Sinvastatina/farmacologia , Animais , Regeneração Óssea , Modelos Animais de Doenças , Quimioterapia Combinada , Durapatita/farmacologia , Feminino , Implantes Experimentais , Ovariectomia , Ratos , Ratos Sprague-Dawley , Titânio/farmacologia
10.
Injury ; 46(11): 2134-41, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26306803

RESUMO

In this study, we tested the effect of Teriparatide (PTH) in combination with ß-tricalcium phosphate (ß-TCP) as a bone void filler in an ovariectomised rat distal femoral metaphysis model.ß-TCP is a completely resorbable synthetic calcium phosphate and the Teriparatide is a drug that can promote bone formation in the condition of osteoporosis. A critical size defect of 3mm in diameter, a through-hole bone defect, was drilled into each distal femur of the ovariectomised rats. The hole was filled with ß-TCP and the rat was injected PTH Teriparatide (30µg/kg) in peritoneum 5 times per week. After 4and 8 weeks the animals were killed and the degree of bone healing analysed. In total, 60 animals were investigated. When the ß-TCP and PTH were used, histological, biochemistry and histomor-phometric evaluations revealed significantly better bone healing in terms of quantity and quality of the newly formed bone. The Ovariectomised rats which suffer from femur metaphysis defect are cured by embedding ß-tricalcuim phosphate and intermittently cured by parathyroid hormone (PTH).


Assuntos
Conservadores da Densidade Óssea/farmacologia , Regeneração Óssea/efeitos dos fármacos , Fosfatos de Cálcio/farmacologia , Fêmur/patologia , Fraturas por Osteoporose/patologia , Teriparatida/farmacologia , Animais , Modelos Animais de Doenças , Feminino , Osteoporose , Fraturas por Osteoporose/tratamento farmacológico , Hormônio Paratireóideo , Ratos , Ratos Sprague-Dawley
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