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1.
ISA Trans ; 2022 Apr 27.
Artigo em Inglês | MEDLINE | ID: mdl-35551818

RESUMO

This correspondence analyses the issue of asynchronous filtering for discrete-time Markov switched repeat scalar nonlinear systems under the deception attacks. Aiming to economize on the finite network bandwidth, a novel event-triggered try-once-discard protocol is presented. At the same time, the hidden Markov model with partial information is considered to describe the asynchronization between Markov switched repeat scalar nonlinear systems and filter. By establishing a mode-dependent diagonally dominant Lyapunov function, sufficient criteria are attained such that the augmented filtering error system is stochastically stable when this system meets an expected H∞ performance level, and the desired asynchronous filter is designed. The correctness and validity of the proposed design method are verified by a numerical example.

2.
Math Biosci Eng ; 19(5): 4719-4736, 2022 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-35430837

RESUMO

Immune checkpoint genes (ICGs) have recently been proven to perform instrumental functions in the maintenance of immune homeostasis and represent a promising therapeutic strategy; however, their expression patterns and prognostic values are not fully elucidated in hepatocellular carcinoma (HCC). In this investigation, we focused on establishing and validating a prognostic gene signature to facilitate decision-making in clinical practice. Clinical information, as well as transcriptome data, was obtained from the Cancer Genome Atlas (TCGA) and International Cancer Genome Consortium (ICGC) database. Univariate Cox regression and least absolute shrinkage and selection operator (LASSO) Cox method were employed to build a multi-gene signature in the TCGA database, while the ICGC database was used for validation. Subsequently, utilizing the six-gene signature, we were able to categorize patients into high- and low-risk groups. In two cohorts, survival analysis findings revealed a dismal outlook for the high-risk group. The receiver operating characteristic curves were utilized to estimate the gene signature's prediction ability. Moreover, correlation analysis showed high-risk group was linked to advanced pathological stage, infiltration of immune cells and therapeutic response. In summary, this unique gene profile might serve not only as a useful prognostic indicator but also as a marker of therapy responsiveness in HCC.


Assuntos
Carcinoma Hepatocelular , Neoplasias Hepáticas , Carcinoma Hepatocelular/genética , Carcinoma Hepatocelular/terapia , Humanos , Neoplasias Hepáticas/genética , Neoplasias Hepáticas/terapia , Curva ROC , Transcriptoma
3.
Mol Biol Evol ; 2022 Apr 21.
Artigo em Inglês | MEDLINE | ID: mdl-35446958

RESUMO

Because errors at DNA level power pathogen evolution, a systematic understanding of the rate and molecular spectrum of mutations could guide the avoidance and treatment of infectious diseases. We thus accumulated tens of thousands of spontaneous mutations in 768 repeatedly bottlenecked lineages of 18 strains from various geographical sites, temporal spread, and genetic backgrounds. Entailing over ∼1.36 million generations, the resultant data yield an average mutation rate of ∼0.0005 per genome per generation, with significant within-species variation. This is one of the lowest bacterial mutation rates reported, giving direct support for a high genome stability in this pathogen resulting from high DNA-mismatch-repair efficiency and replication-machinery fidelity. Pathogenicity genes do not exhibit an accelerated mutation rate, and thus elevated mutation rates may not be the major determinant for the diversification of toxin and secretion systems. Intriguingly, a low error rate at the transcript level is not observed, suggesting distinct fidelity of the replication and transcription machineries. This work urges more attention on the most basic evolutionary processes of even the best-known human pathogens, and deepens the understanding of their genome evolution.

4.
Int J Mol Sci ; 23(7)2022 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-35409186

RESUMO

Catalases (CATs) are important self-originating enzymes and are involved in many of the biological functions of plants. Multiple forms of CATs suggest their versatile role in lesion mimic mutants (LMMs), H2O2 homeostasis and abiotic and biotic stress tolerance. In the current study, we identified a large lesion mimic mutant9428 (llm9428) from Ethyl-methane-sulfonate (EMS) mutagenized population. The llm9428 showed a typical phenotype of LMMs including decreased agronomic yield traits. The histochemical assays showed decreased cell viability and increased reactive oxygen species (ROS) in the leaves of llm9428 compared to its wild type (WT). The llm9428 showed enhanced blast disease resistance and increased relative expression of pathogenesis-related (PR) genes. Studies of the sub-cellular structure of the leaf and quantification of starch contents revealed a significant decrease in starch granule formation in llm9428. Genetic analysis revealed a single nucleotide change (C > T) that altered an amino acid (Ala > Val) in the candidate gene (Os03g0131200) encoding a CATALASE C in llm9428. CRISPR-Cas9 targetted knockout lines of LLM9428/OsCATC showed the phenotype of LMMs and reduced starch metabolism. Taken together, the current study results revealed a novel role of OsCATC in starch metabolism in addition to validating previously studied functions of CATs.


Assuntos
Oryza , Resistência à Doença/genética , Regulação da Expressão Gênica de Plantas , Peróxido de Hidrogênio/metabolismo , Mutação , Oryza/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Amido/metabolismo
5.
Food Chem ; 384: 132622, 2022 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-35413777

RESUMO

Current work proposes an innovative substitute cultivation strategy for Auricularia auricula that connects efficient lignocellulose biodegradation of corn stalk by pretreatment with mycelium growth of Auricularia auricula under solid state fermentation. The growth promotion effect of mycelium was as follows: alkali pretreatment > alkali combined with ozone pretreatment > ozone pretreatment > high-temperature water pretreatment (control). The highest cellulose degradation (35.38%) was observed in alkali pretreatment group (P < 0.05), while the maximum lignin loss of 28.10% was reached in the ozone pretreatment group. Correlation analysis showed that both cellulose and lignin could be used as carbon sources to promote mycelium growth, but the effect of cellulose was better. High activities of ß-glucosidase (2.92 IU/g) and filter paper enzyme (2.92 IU/g) were observed in alkali pretreatment group on Day 6 and 8, respectively, which were significantly correlated with the change of cellulose degradation rate (P < 0.01).


Assuntos
Lignina , Ozônio , Álcalis/química , Auricularia , Biomassa , Celulose/química , Fermentação , Hidrólise , Lignina/química , Ozônio/química , Polissacarídeos/química , Zea mays/química
6.
Biosens Bioelectron ; 210: 114257, 2022 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-35447395

RESUMO

The simultaneous, precise, and quantitative detection of multi-components inflammatory markers (IMs) in sepsis serum by surface-enhanced Raman scattering (SERS) remains a challenging problem. A novel, multifunctional biosensor with dual enrichment and enhancement was designed for the ultrasensitive and quantitative analysis of multi-components IMs. The biosensor contains SERS tags-unique urchin core/porous shell (CPS) structure modified with Raman reporters (RaRs), magnetic assist-Ag coated Fe3O4 magnetic nanoparticles (Ag MNPs) modified with internal standard (IS), and then aptamer (Apt) modification to form the sandwich structure (Ag MNPs/IMs/CPS). This multifunctional sensor used for IMS detection has the following innovations: The intensity ratio IRaRs/IIS with Lg CIMs present a good and wide linear relationship to achieve the simultaneous, precise, and quantitative detection of IMS in serum; The detection results display ultrasensitivity, and the limit of detection (LOD) for CRP, IL-6, and PCT is 100 fg/mL, 0.1 fg/mL, and 1.0 fg/mL, which is lower than other detection techniques; The calculated data of clinical blood samples of sepsis by this SERS method is consistent with the hospital results, and can provide more compositional data of IMs. Thus, this combined approach developed a sensing platform for rapid screening, accurate evaluation, early warning, and diagnosis of sepsis.


Assuntos
Técnicas Biossensoriais , Nanopartículas Metálicas , Sepse , Técnicas Biossensoriais/métodos , Ouro/química , Humanos , Limite de Detecção , Fenômenos Magnéticos , Nanopartículas Metálicas/química , Porosidade , Sepse/diagnóstico , Análise Espectral Raman/métodos
7.
Nanomaterials (Basel) ; 12(7)2022 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-35407262

RESUMO

A long-range surface plasmon polariton (SPP) waveguide, composed of double-layer graphene, can be pivotal in transferring and handling mid-infrared electromagnetic waves. However, one of the key challenges for this type of waveguide is how to excite the SPP modes through an incident light beam. In this study, our proposed design of a novel grating, consisting of a graphene-based cylindrical long-range SPP waveguide array, successfully addresses this issue using finite-difference time-domain simulations. The results show that two types of symmetric coupling modes (SCMs) are excited through a normal incident light. The transmission characteristics of the two SCMs can be manipulated by changing the interaction of the double-layer gratings of graphene as well as by varying various parameters of the device. Similarly, four SCMs can be excited and controlled by an oblique incident light because this light source is equivalent to two orthogonal beams of light. Furthermore, this grating can be utilized in the fabrication of mid-infrared optical devices, such as filters and refractive index sensors. This grating, with double-layer graphene arrays, has the potential to excite and manipulate the mid-infrared electromagnetic waves in future photonic integrated circuits.

8.
Anal Chem ; 94(15): 5785-5796, 2022 04 19.
Artigo em Inglês | MEDLINE | ID: mdl-35343684

RESUMO

New strategies combining sensitive pathogenic bacterial detection and high antimicrobial efficacy are urgently desirable. Here, we report smart triple-functional Au-Ag-stuffed nanopancakes (AAS-NPs) exhibiting (1) controllably oxidative Ag-etching thickness for simultaneously obtaining the best surface-enhanced Raman scattering (SERS) enhancement and high Ag-loading antibacterial drug delivery, (2) expressive Ag+-accelerated releasing capability under neutral phosphate-buffered saline (PBS) (pH ∼ 7.4) stimulus and robust antibacterial effectiveness involving sustainable Ag+ release, and (3) three-in-one features combining specific discrimination, sensitive detection, and inactivation of different pathogenic bacteria. Originally, AAS-NPs were synthesized by particle growth of the selective Ag-etched Au@Ag nanoparticles with K3[Fe(CN)6], followed by the formation of an unstable Prussian blue analogue for specifically binding with bacteria through the cyano group. Using specific bacterial "fingerprints" resulting from the introduction of dual-function 4-mercaptophenylboronic acid (4-MPBA, serving as both the SERS tag and internal standard) and a SERS sandwich nanostructure that was made of bacteria/SERS tags/AAS-NPs, three bacteria (E. coli, S. aureus, and P. aeruginosa) were highly sensitively discriminated and detected, with a limit of detection of 7 CFU mL-1. Meanwhile, AAS-NPs killed 99% of 1 × 105 CFU mL-1 bacteria within 60 min under PBS (pH ∼ 7.4) pretreatment. Antibacterial activities of PBS-stimulated AAS-NPs against S. aureus, E. coli, and P. aeruginosa were extraordinarily increased by 64-fold, 72-fold, and 72-fold versus PBS-untreated AAS-NPs, respectively. The multiple functions of PBS-stimulated AAS-NPs were validated by bacterial sensing, inactivation in human blood samples, and bacterial biofilm disruption. Our work exhibits an effective strategy for simultaneous bacterial sensing and inactivation.


Assuntos
Nanopartículas Metálicas , Prata , Antibacterianos/química , Antibacterianos/farmacologia , Bactérias , Escherichia coli , Ouro/química , Humanos , Nanopartículas Metálicas/química , Antígenos O , Pseudomonas aeruginosa , Prata/química , Prata/farmacologia , Análise Espectral Raman/métodos , Staphylococcus aureus
9.
Transbound Emerg Dis ; 2022 Mar 03.
Artigo em Inglês | MEDLINE | ID: mdl-35243794

RESUMO

From 2003 onwards, three pandemics have been caused by coronaviruses: severe acute respiratory syndrome coronavirus (SARS-CoV); middle east respiratory syndrome coronavirus (MERS-CoV); and, most recently, SARS-CoV-2. Notably, all three were transmitted from animals to humans. This would suggest that animals are potential sources of epidemics for humans. The emerging porcine delta-coronavirus was reported to infect children. This is a red flag that marks the ability of PDCoV to break barriers of cross-species transmission to humans. Therefore, we conducted molecular genetic analysis of global clade PDCoV to characterize spatiotemporal patterns of viral diffusion and genetic diversity. PDCoV was classified into three major lineages, according to distribution and phylogenetic analysis of PDCoV. It can be inferred based on the analysis results of the currently known PDCoV strains that PDCoV might originate in Asia. We also selected six special spike amino acid sequences to align and analyze to find seven significant mutation sites. The accumulation of these mutations may enhance dynamic movements, accelerating spike protein membrane fusion events and transmission. Altogether, our study offers a novel insight into the diversification, evolution, and interspecies transmission and origin of PDCoV and emphasizes the need to study the zoonotic potential of the PDCoV and comprehensive surveillance and enhanced biosecurity precautions for PDCoV.

10.
BMC Ophthalmol ; 22(1): 139, 2022 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-35346124

RESUMO

PURPOSE: To develop a deep learning-based framework to improve the image quality of optical coherence tomography (OCT) and evaluate its image enhancement effect with the traditional image averaging method from a clinical perspective. METHODS: 359 normal eyes and 456 eyes with various retinal conditions were included. A deep learning framework with high-resolution representation was developed to achieve image quality enhancement for OCT images. The quantitative comparisons, including expert subjective scores from ophthalmologists and three objective metrics of image quality (structural similarity index measure (SSIM), peak signal-to-noise ratio (PSNR) and contrast-to-noise ratio (CNR)), were performed between deep learning method and traditional image averaging. RESULTS: With the increase of frame count from 1 to 20, our deep learning method always obtained higher SSIM and PSNR values than the image averaging method while importing the same number of frames. When we selected 5 frames as inputs, the local objective assessment with CNR illustrated that the deep learning method had more obvious tissue contrast enhancement than averaging method. The subjective scores of image quality were all highest in our deep learning method, both for normal retinal structure and various retinal lesions. All the objective and subjective indicators had significant statistical differences (P < 0.05). CONCLUSION: Compared to traditional image averaging methods, our proposed deep learning enhancement framework can achieve a reasonable trade-off between image quality and scanning times, reducing the number of repeated scans.


Assuntos
Aprendizado Profundo , Doenças Retinianas , Humanos , Aumento da Imagem/métodos , Doenças Retinianas/diagnóstico por imagem , Tomografia de Coerência Óptica/métodos
11.
Cancer Cell Int ; 22(1): 97, 2022 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-35193632

RESUMO

BACKGROUND: Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide. Costimulatory molecules have been proven to be the foundation of immunotherapy. However, the potential roles of costimulatory molecule genes (CMGs) in HCC remain unclear. Our study is aimed to develop a costimulatory molecule-related gene signature that could evaluate the prognosis of HCC patients. METHODS: Based on The Cancer Gene Atlas (TCGA) database, univariate Cox regression analysis was applied in CMGs to identify prognosis-related CMGs. Consensus clustering analysis was performed to stratify HCC patients into different subtypes and compared them in OS. Subsequently, the LASSO Cox regression analysis was performed to construct the CMGs-related prognostic signature and Kaplan-Meier survival curves as well as ROC curve were used to validate the predictive capability. Then we explored the correlations of the risk signature with tumor-infiltrating immune cells, tumor mutation burden (TMB) and response to immunotherapy. The expression levels of prognosis-related CMGs were validated based on qRT-PCR and Human Protein Atlas (HPA) databases. RESULTS: All HCC patients were classified into two clusters based on 11 CMGs with prognosis values and cluster 2 correlated with a poorer prognosis. Next, a prognostic signature of six CMGs was constructed, which was an independent risk factor for HCC patients. Patients with low-risk score were associated with better prognosis. The correlation analysis showed that the risk signature could predict the infiltration of immune cells and immune status of the immune microenvironment in HCC. The qRT-PCR and immunohistochemical results indicated six CMGs with differential expression in HCC tissues and normal tissues. CONCLUSION: In conclusion, our CMGs-related risk signature could be used as a prediction tool in survival assessment and immunotherapy for HCC patients.

12.
Tissue Eng Part C Methods ; 28(3): 127-135, 2022 03.
Artigo em Inglês | MEDLINE | ID: mdl-35172637

RESUMO

Alveolar cleft repair is a key step in multiple disciplinary treatment for patients with cleft lip/and palate. Although autologous bone grafting has been used worldwide over the past half century, alternative advanced techniques, such as the use of bone substitutes and guided tissue regeneration, have shown their great potentials and have been recommended by a growing number of physicians and surgeons. The employment of new therapeutic approaches and devices in clinical routine requires tremendous experimental efforts and appropriate animal models with similar sizes and sites of deformity to that of human both anatomically and physiologically. The aim of this study is to develop a juvenile porcine model with surgically created alveolar clefts imitating congenital alveolar cleft in the cleft lip and palate. Alveolar defects between second incisor and canine were surgically created in two miniature pigs (unilateral cleft in P1 and P2); bilateral alveolar defects were surgically created between first and third incisor in one miniature pig (P3) using piezo surgery. Pigs were sacrificed (P1 at 1 month after the surgery and P2 at 3 months postoperatively) and the evaluation of defects were performed by assessing result from the computed tomography (CT) scan and histopathological examination. Postoperative CT scan results showed that the size of the defect remained the same, whereas the edge of the defect became irregular 3 months after the surgery. In all pig subjects, histopathological examination found no sign of osteogenesis in the area of defect, indicating that our surgical procedure was successful in establishing porcine models for alveolar cleft in congenital cleft lip and palate. In conclusion, we developed alveolar cleft in porcine models to mimic the size, site, and environment of congenital alveolar cleft in cleft lip and palate. The novel animal model can be employed in pilot studies for the purpose of optimizing the current surgical treatment techniques as well as developing new treatment procedures and test the bone substitute materials. The bilateral model can be applied in further control studies. Impact statement Cancellous iliac bone graft was the most popular surgical technique as well as the gold standard to reconstruct alveolar cleft. Nevertheless, several disadvantages exist regarding the additional surgical field of donor side and delayed age of alveolar bone grafting. Bone tissue-engineered strategy offers a promising alternative to address the gap in the current limitation of autologous bone to treat the growing craniofacial skeleton. Among different species of laboratory animals, porcine is suitable for oral and maxillofacial bone and implant-related research, where alveolar defect can be surgically developed simulating the size and site of alveolar cleft occurring together with cleft lip and palate. In this proposal, a reproducible porcine model of alveolar bone defect imitating congenital alveolar cleft during craniofacial growing stage is successfully constructed that will show great potential application in the field of tissue engineering and regenerative medicine. The model for bilateral alveolar cleft can be potentially applied in a controlled study in future.


Assuntos
Fenda Labial , Fissura Palatina , Animais , Transplante Ósseo/métodos , Fenda Labial/cirurgia , Fissura Palatina/patologia , Fissura Palatina/cirurgia , Humanos , Comportamento Imitativo , Suínos
13.
J Colloid Interface Sci ; 614: 629-641, 2022 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-35123215

RESUMO

It's known that the application of bismaleimide resins (BMI) is limited due to its brittleness and poor flame retardancy. A novel type of BMI monomer (MADQ) based on the typical phosphorus series flame retardant DOPO is designed to improve the fire safety of BMI. Besides, aliphatic long chain structure is introduced in MADQ, which is supposed to be conducive to reducing the rigidity of the BMI cross-linked network and thus to improve the toughness of BMI. It's seen that with the incorporation of 5.24 wt% MADQ, the peak of heat release rate (PHRR) and total heat release (THR) of resultant BMI/MADQ-5 is reduced by 37.7% and 33.9%, respectively. Meanwhile, with modification of 1.07 wt% MADQ, BMI/MADQ-1 possesses UL-94V-0 rating. The relevant mechanism analysis reveals that the phosphaphenanthrene group in MADQ can exert flame retardancy effect both in condensed and gas phase. Besides, the impact strength of the BMI/MADQ is maximally increased by nearly 90.1%. Furthermore, the BMI/MADQ still maintains high tensile strength and thermal stability, which indicates the modification of MADQ did not deteriorate other properties of BMI. An innovative research idea and research basis for the preparation of intrinsic flame-retardant and toughened BMI is provided in this work.


Assuntos
Retardadores de Chama , Temperatura Alta , Fósforo
14.
J Craniofac Surg ; 2022 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-35067530

RESUMO

Submucous cleft palate, presenting as varying degrees of palatal bony defect, can be difficult to detect in its early stage. The connection between submucous cleft palate and cleft lip has been noticed by clinicians but are rarely reported in literature. AIMS: To investigate the correlation between the degree of deformity of palatal bony defect and that of cleft lip. PATIENTS AND METHODS: Thirty-four patients with unilateral (n = 23) or bilateral (n = 11) cleft lip presenting with submucous cleft palate were included. Patients were divided into 3 groups according to the degree of malformation of cleft lip (microform, incomplete, and complete). The length and width of palatal bony defect was then measured from the palatal computer tomography. RESULTS: In patients with unilateral cleft lip, the proportions of microform cleft lip, incomplete cleft lip, and complete cleft lip were 17.4%, 60.9%, and 21.7%, respectively. In patients with bilateral cleft lip, there were 3 cases with microform and 1 case with incomplete cleft lip on both sides. No correlation was found between the length or relative width of palatal bony defect with the side (P length = 1.000; P relative width = 0.262) or the severity (P length = 0.605; P relative width = 0.254) of cleft lip. CONCLUSIONS: The form of cleft lip presenting with submucous cleft palate varies, and there was no correlation with the length or relative width of palatal bony defect. Advanced imaging techniques for children with cleft lip may assist the early diagnosis of submucous cleft palate.

15.
Mol Biol Rep ; 49(3): 1945-1954, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-34843038

RESUMO

AIM: To investigate the association of serum exosomes miR-574-5p with liver fibrosis, and explore the effect and mechanism of serum exosomes on HSC activation. MATERIALS AND METHODS: Using serum samples collected from healthy adults and patients with liver cirrhosis, we extracted human serum exosomes via ultra-high-speed centrifugation, and co-cultured them with hepatic stellate cells (HSCs) line LX2. LX-2-mediated intake of human serum exosomes was examined by confocal microscopy. To induce liver fibrosis, we administered 20% CCl4 to mice intraperitoneally and adopted an exoEasy MIDI kit to extract serum exosomes.Liver fibrosis-related molecules were determined via qRT-PCR, Western blot, Masson staining, and Immunohistochemical staining. RESULTS: Significantly high miR-574-5p levels were expressed in serum exosomes and were positively correlated with the expression of miR-574-5p, collagen deposition, and α-SMA expression in liver tissues of mice during liver fibrosis. Compared to healthy subjects, serum exosomes from cirrhosis patients were associated with higher expression of miR-574-5p. MiR-574-5p mimic promoted the expression of α-SMA and COL1A1 mRNA and protein in LX-2, whereas miR-574-5p inhibitor exerted no effect. CONCLUSION: This article demonstrates that miR-574-5p expression in serum exosomes is positively correlated with collagen deposition and HSC activation in liver tissues during liver fibrosis.Serum exosomes potentially activate HSC through the transfer of miR-574-5p to HSC during liver fibrosis.


Assuntos
Exossomos , MicroRNAs , Animais , Linhagem Celular , Proliferação de Células , Exossomos/metabolismo , Células Estreladas do Fígado/metabolismo , Humanos , Cirrose Hepática/patologia , Camundongos , MicroRNAs/metabolismo
16.
J Colloid Interface Sci ; 606(Pt 2): 1395-1409, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34492475

RESUMO

As an antioxidant, hindered phenol scavenges free radicals. Due to the oxidative degradation of black phosphorus (BP) in the presence of water and oxygen, free radical quenching of hindered phenol antioxidants can solve this issue and improve the environmental stability and flame retardant efficiency of BP. Herein, hydroxyl-modified BP (BP-OH) with active groups on the surface was obtained by hydroxylation, and then the hindered phenol antioxidant was grafted onto the surface of BP-OH through an isophorone diisocyanate bridging covalent reaction to obtain hindered phenol-modified BP (BP-HPL). The fire hazard of thermoplastic polyurethane (TPU) can be significantly reduced by introducing BP-HPL into TPU. Adding 2 wt% BP-HPL can reduce the heat release rate and total heat release values of TPU by 49.9% and 49.0%, respectively. In addition, the reductions in smoke volume and carbon monoxide production were also significant. Compared with BP-OH, the environmental stability of BP-HPL is significantly improved. This work provides a reference for the application of BP in the field of fire safety and simultaneously achieves the improvement of the environmental stability and flame retardant performance of BP.


Assuntos
Antioxidantes , Fósforo , Radicais Livres , Fenóis , Poliuretanos
17.
Physiol Rep ; 10(1): e15160, 2022 01.
Artigo em Inglês | MEDLINE | ID: mdl-35005850

RESUMO

Soluble lectin-like oxidized low-density lipoprotein receptor-1 (sLOX-1) and lipoprotein-associated phospholipase A2 (Lp-PLA2) plays an important role in acute cerebral infarction (ACI), whereas its clinical value in predicting the prognosis is unclear. Thus, this study aimed to explore this issue. A total of 127 ACI patients were included in this prospective observational study. The concentrations of sLOX-1 and Lp-PLA2 in serum were measured and their relationship with a poor prognosis 90 days after the onset of ACI was analyzed. We found that patients with poor prognosis had higher mean serum levels of sLOX-1 and Lp-PLA2. The level of sLOX-1 and Lp-PLA2 could predict the functional outcome of ACI. At the optimal cut off value of sLOX-1 level (1257.92 ng/ml), the sensitivity and specificity for the poor functional outcome were 0.69 and 0.753, respectively, and the area under ROC curve (AUC) was 0.727. Similarly, the optimal value for Lp-PLA2 level was 160.9 ng/ml, at which the sensitivity and specificity were 0.643 and 0.835, respectively; and the AUC was 0.758. When the two biomarkers were used in combination, the AUC was 0.855, and the sensitivity and specificity were 0.643 and 0.976, respectively, indicating a significant improvement of the diagnostic specificity. The level of sLOX-1 or Lp-PLA2 could thus serve as useful biomarkers to predict the functional outcome of ACI. Combined use of both indicators is better than the use of either single indicator, and provides the highest specificity in predicting poor prognosis.


Assuntos
1-Alquil-2-acetilglicerofosfocolina Esterase , Isquemia Encefálica , Doença Aguda , Biomarcadores , Infarto Cerebral/diagnóstico , Humanos , Prognóstico , Fatores de Risco
18.
World J Clin Cases ; 9(31): 9440-9451, 2021 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-34877279

RESUMO

BACKGROUND: The identification of risk factors for recurrence in patients with minor ischemic stroke (MIS) is a critical medical need. AIM: To develop a nomogram for individualized prediction of in-hospital recurrence in MIS patients. METHODS: Based on retrospective collection, a single-center study was conducted at the First Affiliated Hospital of Anhui Medical University from January 2014 to December 2019. Univariate and multivariate logistic regression analyses were used to determine the risk factors associated with MIS recurrence. The least absolute shrinkage and selection operator regression was performed for preliminary identification of potential risk factors. Uric acid, systolic blood pressure, serum total bilirubin (STBL), and ferritin were integrated for nomogram construction. The predictive accuracy and calibration of the nomogram model were assessed by the area under the receiver operating characteristic curve (AUC-ROC) and Hosmer-Lemeshow test, respectively. RESULTS: A total of 2216 MIS patients were screened. Among them, 155 were excluded for intravascular therapy, 146 for unknown National Institutes of Health Stroke Scale score, 195 for intracranial hemorrhage, and 247 for progressive stroke. Finally, 1244 patients were subjected to further analysis and divided into a training set (n = 796) and a validation set (n = 448). Multivariate logistic regression analysis revealed that uric acid [odds ratio (OR): 0.997, 95% confidence interval (CI): 0.993-0.999], ferritin (OR: 1.004, 95%CI: 1.002-1.006), and STBL (OR: 0.973, 95%CI: 0.956-0.990) were independently associated with in-hospital recurrence in MIS patients. Our model showed good discrimination; the AUC-ROC value was 0.725 (95%CI: 0.646-0.804) in the training set and 0.717 (95%CI: 0.580-0.785) in the validation set. Moreover, the calibration between nomogram prediction and the actual observation showed good consistency. Hosmer-Lemeshow test results confirmed that the nomogram was well-calibrated (P = 0.850). CONCLUSION: Our present findings suggest that the nomogram may provide individualized prediction of recurrence in MIS patients.

19.
Insects ; 12(12)2021 Dec 17.
Artigo em Inglês | MEDLINE | ID: mdl-34940220

RESUMO

This study aimed to clarify the functional response and control potential of O. sauteri in relation to tea thrips. The functional response, interference response, and control potential of O. sauteri on adult tea thrips, in different insect stages and environment temperatures, were studied. The results showed that the predation of O. sauteri against tea thrips was positively correlated with prey density, while the effects of searching for O. sauteri on the adult tea thrips were negatively correlated with prey density. The predation effects of O. sauteri on tea thrips were also influenced by prey density, which indicated that there was an intra-specific interference response from predators to tea thrips. The population density of tea thrips was significantly decreased, and O. sauteri showed a remarkable ability to control them when the benefit-to-harm ratio was 3:100.

20.
Front Genet ; 12: 790426, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34956331

RESUMO

Atrial septal defects (ASDs) are the most common types of cardiac septal defects in congenital heart defects. In addition to traditional therapy, interventional closure has become the main treatment method. However, the molecular events and mechanisms underlying the repair progress by occlusion device remain unknown. In this study, we aimed to characterize differentially expressed genes (DEGs) in the blood of patients treated with occlusion devices (metal or poly-L-lactic acid devices) using RNA-sequencing, and further validated them by qRT-PCR analysis to finally determine the expression of key mediating genes after closure of ASD treatment. The result showed that total 1,045 genes and 1,523 genes were expressed differently with significance in metal and poly-L-lactic acid devices treatment, respectively. The 115 overlap genes from the different sub-analyses are illustrated. The similarities and differences in gene expression reflect that the body response process involved after interventional therapy for ASDs has both different parts that do not overlap and the same part that crosses. The same portion of body response regulatory genes are key regulatory genes expressed in the blood of patients with ASDs treated with closure devices. The gene ontology enrichment analysis showed that biological processes affected in metal device therapy are immune response with CXCR4 genes and poly-L-lactic acid device treatment, and the key pathways are nuclear-transcribed mRNA catabolic process and proteins targeting endoplasmic reticulum process with ribosomal proteins (such as RPS26). We confirmed that CXCR4, TOB1, and DDIT4 gene expression are significantly downregulated toward the pre-therapy level after the post-treatment in both therapy groups by qRT-PCR. Our study suggests that the potential role of CXCR4, DDIT4, and TOB1 may be key regulatory genes in the process of endothelialization in the repair progress of ASDs, providing molecular insights into this progress for future studies.

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