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Ying Yong Sheng Tai Xue Bao ; 25(5): 1513-7, 2014 May.
Artigo em Chinês | MEDLINE | ID: mdl-25129956


To evaluate the influence of different host plants including apple, wild jujube, jujube, pear and hawthorn on the cold-tolerance substances in overwintering larvae of the peach fruit moth Carposina sasakii Matsumura, we measured the larvae super-cooling capacity, the water content (W), total fat content (TFC), total protein content (TPC) and total glycogen content (TGC) in the body. Results showed that the mean super-cooling point (SCPs) and freezing point (FPs) of overwintering larvae from the 5 host plant fruits differed significantly, ranging from -15.53 to -8.50 degrees C and -11.31 to -4.04 degrees C, respectively. The overwintering larvae fed on hawthorn owned the highest SCP, FP, TGC and the lowest W, while those fed on apple had the lowest SCP, FP, TFC and TGC but the highest W and TPC. The fresh mass (FM) of the overwintering larvae fed on pear was the highest, while those fed on jujube was very low. Those fed on jujube accumulated the highest TFC but the lowest TPC.

Temperatura Baixa , Mariposas/fisiologia , Animais , Congelamento , Frutas , Glicogênio , Proteínas de Insetos , Larva , Malus , Prunus , Estações do Ano , Ziziphus
Biol Chem ; 394(1): 43-54, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-23096575


Chemosensory proteins (CSPs) are small soluble proteins often associated with chemosensory organs in insects but include members involved in other functions, such as pheromone delivery and development. Although the CSPs of the sensory organs have been extensively studied, little is known on their functions in other parts of the body. A first screening of the available databases has identified 70 sequences encoding CSPs in the oriental locust Locusta migratoria manilensis. Applying proteomic analysis, we have identified 17 of them abundantly expressed in the female reproductive organs, but only one (CSP91) in male organs. Bacterially expressed CSP91 binds fatty acids with a specificity for oleic and linoleic acid, as well as medium-length alcohols and esters. The same acids have been detected as the main gas chromatographic peaks in the dichloromethane extracts of reproductive organs of both sexes. The abundance and the number of CSPs in female reproductive organs indicates important roles for these proteins. We cannot exclude that different functions can be associated with each of the 17 CSPs, including delivery of semiochemicals, solubilization of hormones, direct control of development, or other unknown tasks.

Regulação da Expressão Gênica , Genitália/metabolismo , Proteínas de Insetos/genética , Proteínas de Insetos/metabolismo , Locusta migratoria/anatomia & histologia , Locusta migratoria/metabolismo , Caracteres Sexuais , Animais , Feminino , Regulação da Expressão Gênica/genética , Proteínas de Insetos/análise , Masculino , Proteômica
Ying Yong Sheng Tai Xue Bao ; 23(7): 1933-9, 2012 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-23173470


To understand the resistance risks of Frankliniella occidentalis Pergande against phoxim, this paper studied the resistance mechanisms of phoxim-resistant F. occidentalis population against phoxim and the cross-resistance of the population against other insecticides. The phoxim-resistant population had medium level cross-resistance to chlorpyrifos, lambda-cyhalothrin, and methomyl, low level cross-resistance to chlorfenapyr, imidacloprid, emamectin-benzoate, and spinosad, but no cross-resistance to acetamiprid and abamectin. The synergists piperonyl butoxide (PBO), s, s, s-tributyl phosphorotrithioate (DEF), and triphenyl phosphate (TPP) had significant synergism (P < 0.05) on the toxicity of phoxim to the resistant (XK), field (BJ), and susceptible (S) populations, while diethyl maleate (DEM) had no significant synergism to XK and S populations but had significant synergism to BJ population. As compared with S population, the XK and BJ populations had significantly increased activities of mixed-functional oxidases P450 (2.79-fold and 1.48-fold), b, (2.88-fold and 1.88-fold), O-demethylase (2.60-fold and 1.68-fold), and carboxylesterase (2.02-fold and 1.61-fold, respectively), and XK population had a significantly increased acetylcholine esterase activity (3.10-fold). Both XK and BJ population had an increased activity of glutathione S-transferases (1.11-fold and 1.20-fold, respectively), but the increment was not significant. The increased detoxification enzymes activities in F. occidentalis could play an important role in the resistance of the plant against phoxim.

Resistência a Inseticidas , Inseticidas/farmacologia , Compostos Organotiofosforados/farmacologia , Tisanópteros/efeitos dos fármacos , Tisanópteros/crescimento & desenvolvimento , Acetilcolinesterase/metabolismo , Animais , Clorpirifos/farmacologia , Metomil/farmacologia , Nitrilos/farmacologia , Piretrinas/farmacologia , Tisanópteros/enzimologia